201. Characterization of the microbial community, metabolome and biotransformation of phenolic compounds of sainfoin (Onobrychis viciifolia) silage ensiled with or without inoculation of Lactobacillus plantarum.
- Author
-
Xu, Dongmei, Ding, Zitong, Wang, Museng, Bai, Jie, Ke, Wencan, Zhang, Yixin, and Guo, Xusheng
- Subjects
- *
PHENOLS , *LACTOBACILLUS plantarum , *ISOFLAVONES , *SILAGE , *ANTHOCYANINS , *FLAVONES - Abstract
• Lactobacillus acetotolerans was the dominant bacterium in control sainfoin silage. • Lactobacillus buchneri was the dominant bacterium in L. plantarum treated-silage. • Ensiling increased flavones, flavonols, flavonoids, flavanones, and isoflavones. • Ensiling decreased concentrations of 32 flavones and 11 anthocyanins in sainfoin. • Inoculant L. plantarum decreased concentrations of 10 flavones in sainfoin silage. The present study investigated the bacterial community, metabolome and biotransformation of phenolic compounds in sainfoin silage inoculated either with or without Lactobacillus plantarum. Our results revealed that the epiphytic microbiota of wilted sainfoin mainly comprised Acinetobacter , Spingomonas , Pseudomonas , Lactobacillus , Delftia and Aquabacterium. After ensiling, L. acetotolerans , L. buchneri , L. plantarum , L. pentosus and Clostridium tyrobutricum were the dominant species. Compared to the control, the inoculant L. plantarum increased the relative abundance of L. buchneri while decreased that of the other dominant species. The ensiling process increased the concentrations of 21 flavones, 16 flavonols, 16 flavonoids, 14 flavanones and 9 isoflavones while decreased other 32 flavones and 11 anthocyanins, which mainly modulated by the "isoflavonoid biosynthesis" and "flavonoid biosynthesis" pathways. The inoculant L. plantarum mainly reduced the concentrations of 10 flavones. Compared with the control, the inoculant L. plantarum mainly affected the metabolism pathways related to carbohydrates and nitrogen. [ABSTRACT FROM AUTHOR] more...
- Published
- 2020
- Full Text
- View/download PDF