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The aim of this study was to compare quadriceps concentric and eccentric strength and muscle architecture in chronic obstructive pulmonary disease (COPD) patients vs healthy men. Thirty-five COPD patients (age = 65 ± 4 yrs, forced expiratory volume (FEV1) = 63 ± 10%; FEV1/forced vital capacity (FVC)=57 ± 13% of predicted) and 25 age-matched healthy men (age = 65 ± 4 yrs, FEV1=114 ± 17%; FEV1/FVC = 101 ± 6% of predicted) (CON) participated in the present cross-sectional study. Concentric and eccentric isokinetic peak-torque was measured at low and high angular-velocity. Vastus lateralis pennation angle, fascicle length and muscle thickness were recorded using ultrasound device. Similar eccentric peak-torque was found in COPD and CON at low (2.57 ± 0.55 and 2.80 ± 0.60 N⋅m⋅kg-1, p = 0.128 respectively) and high (2.44 ± 0.51 and 2.58 ± 0.46 N⋅m⋅kg-1, p = 0.259) angular-velocity. Lower concentric peak-torque was found in COPD than in CON (p < 0.05). Smaller pennation angle (13.8 ± 3.4 vs 16.2 ± 3.9°) and muscle thickness (17.1 ± 2.8 vs 20.3 ± 3.0 mm) were found in COPD patients vs healthy men, with no difference in fascicle length. In COPD patients only, FEV1 and FEV1/FVC were negatively correlated with the eccentric-to-concentric peak-torque ratio (r = -0.465 and r = -0.414, respectively); irrespective of the testing-modality, FEV1 and FEV1/FVC were moderately correlated with peak-torque (p < 0.05). The preserved eccentric strength in COPD patients could be accounted for both mechanical and neural adaptations caused by the disease severity.

Spastic paresis is often understood as a neurologic disorder of the motor command that includes agonist paresis and antagonist overactivity. However, a second disorder, involving the muscle and named spastic myopathy, appears rapidly during the acute phase after the lesion. Hypo-mobilization in shortened position of some muscles of the paretic limbs, in the context of paresis of their antagonists, will reduce their longitudinal tension, which acts as the first event of a cascade of transformations involving genetic, structural, biomechanical, and then physiological mechanisms, leading to loss of muscle extensibility and length. At the subacute and chronic stages of this syndrome, the neurological and the muscular disorders coexist, seemingly feeding on each other.From a pathophysiological point of view, this research work started by exploring the entanglements between the neurological and muscular disorders, and the potential responsibility of spastic myopathy in the impairment of active function. The first study demonstrated that the loss of muscle extensibility in antagonists, when above a certain threshold, correlates with the degree of disturbance of the motor command directed to the agonist. The chronology of events established in the literature, with histological muscle abnormalities emerging always before the first expressions of motoneuronal overactivity, suggests a causal role of the muscle disorder in a part of the descending command disorder. The crucial role of the muscle disorder in the syndrome of spastic paresis should encourage clinicians to direct treatment towards techniques to stimulate muscle plasticity.Despite the classic use of muscle stretching in daily practice, its potential to increase muscle extensibility remains a subject of controversy, even though its long-term effects, i.e. over six months of daily implementation, have not been investigated. The second part of this work was thus therapeutic. We retrospectively explored the long-term effects of a daily, high load self-stretching programme within the Guided Self-rehabilitation Contract method, for at least a year. This work showed that this programme, applied over three years in paretic subjects, was increasingly associated with major gains in clinical muscle extensibility. A randomized controlled trial against conventional therapy, which used ultrasound exploration of structural parameters in the stretched muscles, demonstrated that this programme, applied over one year in subjects with stroke-induced chronic hemiparesis, enabled greater increase in plantar flexor fascicle length and active function improvement than conventional therapy.Taken together, these results will contribute to enhancing the knowledge about the evolving structural and mechanical muscle changes in spastic paresis, as a pathologic entity, spastic myopathy. Spastic myopathy needs to be specifically addressed, as it interacts with the neurological disorder and worsens functional impairment. Daily postures of high load self-stretch, guided by the therapist and self-monitored on a diary, should be prescribed and practiced over the long term, in order to treat spastic myopathy in subjects with spastic paresis., La parésie spastique est souvent envisagée comme une atteinte de la commande motrice, comportant une parésie de l’agoniste et une hyperactivité de l’antagoniste. Cependant, une seconde affection d’ordre musculaire - la myopathie spastique, apparait rapidement, pendant la phase aigüe après la lésion. L’hypomobilisation en position courte de certains muscles dans le contexte d’une parésie des muscles opposés s’accompagne d’une perte de leur tension longitudinale, première étape d’une cascade de transformations génétiques, structurelles, biomécaniques puis physiologiques des muscles hypomobilisés, incluant entre autres une perte de leur extensibilité et de leur longueur. Aux stades subaigu puis chronique du syndrome, les affections neurologique et musculaire coexistent, et semblent s’entretenir mutuellement.Sur le plan physiopathologique, ce travail de recherche s’est d’abord intéressé à ces intrications entre les affections neurologique et musculaire, et à la part de responsabilité potentielle de la myopathie spastique dans la fonction active. Ce premier travail a montré que le degré d’hypoextensibilité musculaire d’un antagoniste, au-delà d’un certain seuil, est corrélé au degré de perturbation de la commande motrice dirigée sur l’agoniste. La chronologie des différents événements établie dans la littérature, avec des anomalies histologiques musculaires qui apparaissent toujours avant les premières manifestions d’hyperactivité motoneuronale, amène à suggérer une responsabilité causale de la maladie du muscle dans une partie des perturbations ultérieures de la commande neuronale descendante. Ce rôle probablement essentiel de la maladie musculaire au sein de la parésie spastique doit inciter le clinicien à orienter une part significative de son traitement vers une stimulation optimale de la plasticité musculaire pour tenter de prévenir ou d’inverser le processus de myopathie spastique.Si l’étirement est depuis très longtemps une technique courante, sa capacité à augmenter durablement l’extensibilité musculaire est aujourd’hui controversée, même si ses effets à long terme, i.e. au-delà de six mois d’application quotidienne, n’ont pas été explorés.La deuxième partie, thérapeutique, de ce travail a donc été l’étude des effets à long terme d’un programme quotidien d’auto-étirements de haute intensité au sein de la méthode des Contrats d’Autorééducation Guidée, pendant un an ou plus. Il a d’abord été rétrospectivement montré que ce programme avait permis, sur trois années de suivi de sujets parétiques, une amélioration progressive importante de l’extensibilité musculaire mesurée cliniquement. Un essai randomisé contrôlé contre la kinésithérapie conventionnelle avec mesures échographiques de paramètres structuraux des muscles étirés a ensuite établi que l’application de ce programme durant un an chez des sujets atteints d’hémiparésie chronique sur AVC générait une augmentation de la longueur fasciculaire des muscles fléchisseurs plantaires, parallèlement à une amélioration de la fonction active, de façon nettement plus importante que la thérapie conventionnelle.L’ensemble de ces résultats doit participer à une meilleure prise en compte des altérations passives structurelles évolutives du muscle comme une entité nosologique, la myopathie spastique, méritant un traitement spécifique au sein de la parésie spastique, afin de limiter ses interactions avec la maladie neurologique et de réduire les déficits fonctionnels. La pratique quotidienne de postures d’auto-étirement à haute intensité, guidée par un thérapeute et auto-documentée par le patient sur un registre, peut être prescrite et réalisée sur le long terme, afin de traiter la myopathie spastique.

Skeletal muscle structural assembly (and its remodeling in response to loading-unloading states) can be investigated macroscopically by assessing muscle architecture, described as fascicle geometric disposition within the muscle. Over recent decades, various medical imaging techniques have been developed to facilitate the in vivo assessment of muscle architecture. However, the main advantages and limitations of these methodologies have been fragmentally discussed. In the present article, the main techniques used for the evaluation of muscle architecture are presented: conventional B-mode ultrasonography, extended-field-of-view ultrasound, 3-D ultrasound and magnetic resonance imaging-based diffusion tensor imaging. By critically discussing potentials and shortcomings of each methodology, we aim to provide readers with an overview of both established and new techniques for the in vivo assessment of muscle architecture. This review may serve as decision guidance facilitating selection of the appropriate technique to be applied in biomedical research or clinical routine.

Resistance training may differentially affect morphological adaptations along the length of uni-articular and bi-articular muscles. The purpose of this study was to compare changes in muscle morphology along the length of the rectus femoris (RF) and vastus lateralis (VL) in response to resistance training. Following a 2-wk preparatory phase, 15 resistance-trained men (24.0 ± 3.0 y, 90.0 ± 13.8 kg, 174.9 ± 20.7 cm) completed pre-training (PRE) assessments of muscle thickness (MT), pennation angle (PA), cross-sectional area (CSA), and echo-intensity in the RF and VL at 30, 50, and 70% of each muscle's length; fascicle length (FL) was estimated from respective measurements of MT and PA within each muscle and region. Participants then began a high intensity, low volume (4 x 3-5 repetitions, 3min rest) lower-body resistance training program, and repeated all PRE-assessments after 8 weeks (2 d ∙ wk-1) of training (POST). Although three-way (muscle [RF, VL] x region [30, 50, 70%] x time [PRE, POST]) repeated measures analysis of variance did not reveal significant interactions for any assessment of morphology, significant simple (muscle x time) effects were observed for CSA (p = 0.002) and FL (p = 0.016). Specifically, average CSA changes favored the VL (2.96 ± 0.69 cm2, p < 0.001) over the RF (0.59 ± 0.20 cm2, p = 0.011), while significant decreases in average FL were noted for the RF (-1.03 ± 0.30 cm, p = 0.004) but not the VL (-0.05 ± 0.36 cm, p = 0.901). No other significant differences were observed. The findings of this study demonstrate the occurrence of non-homogenous adaptations in RF and VL muscle size and architecture following 8 weeks of high-intensity resistance training in resistance-trained men. However, training does not appear to influence region-specific adaptations in either muscle.

It has been recurrently observed that, for compound muscle action potentials (M wave) recorded over the innervation zone of the vastus lateralis, the descending portion of the first phase generally shows an 'inflection' or 'shoulder'. We sought to clarify the electrical origin of this shoulder-like feature and examine its implications. M waves evoked by maximal single shocks to the femoral nerve were recorded in monopolar and bipolar configurations from 126 individuals using classical (10-mm recording diameter, 20-mm inter-electrode distance) electrodes and from eight individuals using small electrodes arranged in a linear array. The changes of the M-wave waveform at different positions along the muscle fibers' direction were examined. The shoulder was identified more frequently in monopolar (97%) than in bipolar (46%) M waves. The shoulder of M waves recorded at different distances from the innervation zone had the same latency. Furthermore, the shoulder of the M wave recorded over the innervation zone coincided in latency with the positive peak of that recorded beyond the muscle. The positive phase of the M wave detected 20 mm away from the innervation zone was essentially composed of non-propagating components. The shoulder-like feature in monopolar and bipolar M waves results from the termination of action potentials at the superficial aponeurosis of the vastus lateralis. We conclude that, only the amplitude of the first phase, and not the second, of M waves recorded monopolarly and/or bipolarly in close proximity to the innervation zone can be used reliably to monitor possible changes in muscle membrane excitability. This work has been supported by the Spanish Ministerio de Economía y Competitividad (MINECO), under the TEC2014-58947-R project.

The objective of this study is to determine the relationships between muscle architectures and jumping abilities. Thirty recreationally active men (mean age = 22.21 ± 1.59 years old) were recruited as study participants and underwent testing for muscle architecture and jumping abilities. Ultrasonography method was used to measure fascicle length, pennation angle and muscle thickness of biceps femoris (BF) and vastus lateralis (VL) for muscle architecture variables. Vertical jump and standing long jump were tested for jumping abilities. Pearson Correlation was used to determine the relationship between all muscle architectures and the jumping abilities tested. Results showed that the muscle thickness and pennation angle of BF and the muscle thickness of VL were shown to be positively correlated while the fascicle length of VL was shown to be negatively correlated with vertical jump performance. Besides that, muscle thickness of BF and the pennation angle of VL and BF were shown to be positively correlated with standing long jump performance. Results demonstrated the advantages of having thicker and more pennated muscle fascicles in enhancing jumping abilities.

Skeletal muscle bulges when it contracts. These three-dimensional (3D) dynamic shape changes play an important role in muscle performance by altering the range of fascicle velocities over which a muscle operates. However traditional muscle models are one-dimensional (1D) and cannot fully explain in vivo shape changes. In this study we compared medial gastrocnemius behaviour during human cycling (fascicle length changes and rotations) predicted by a traditional 1D Hill-type model and by models that incorporate two-dimensional (2D) and 3D geometric constraints to in vivo measurements from B-mode ultrasound during a range of mechanical conditions ranging from 14 to 44 N m and 80 to 140 r.p.m. We found that a 1D model predicted fascicle lengths and pennation angles similar to a 2D model that allowed the aponeurosis to stretch, and to a 3D model that allowed for aponeurosis stretch and variable shape changes to occur. This suggests that if the intent of a model is to predict fascicle behaviour alone, then the traditional 1D Hill-type model may be sufficient. Yet, we also caution that 1D models are limited in their ability to infer the mechanisms by which shape changes influence muscle mechanics. To elucidate the mechanisms governing muscle shape change, future efforts should aim to develop imaging techniques able to characterize whole muscle 3D geometry in vivo during active contractions.

New Findings What is the central question of this study? Does contraction influence the fascicle length, pennation angle and effective physiological cross-sectional area (effPCSA) of the quadriceps femoris muscle? Is there a stronger relationship between effPCSA and maximal strength if effPCSA is measured during maximal contraction rather than at rest? What is the main finding and its importance? Fascicle length decreased, pennation angle increased and effPCSA increased in a non-linear manner with isometric torque. The effPCSA during maximal contraction and rest were correlated in a similar manner to maximal strength. The effPCSA at rest is sufficient to characterize the muscle size–strength relationship. The primary purpose of this study was to document the influence of muscle contraction on quadriceps femoris (QF) muscle architecture [fascicle length (Lf) and pennation angle (θp)] and effective physiological cross-sectional area (effPCSA). Secondarily, we aimed to determine whether effPCSA measured during maximal voluntary contraction (MVC) had a stronger relationship to maximal strength than effPCSA at rest. Fifteen young men performed a series of voluntary knee-extension isometric ramp contractions. Isometric maximal voluntary torque (MVT) was recorded during separate MVCs. Measurements of architecture and effPCSA of each constituent muscle of the QF and, subsequently, the whole QF were made at rest, during 20% increments of maximal voluntary torque and during an MVC. The QF muscle architecture and morphology changed in a curvilinear manner with relative torque (%MVT), with significant differences being observed between incremental torque levels for Lf, θp and effPCSA. Specifically, from rest to MVC, QF Lf decreased (–23.5 ± 3.3%), whereas θp increased (+39.7 ± 6.6%). The QF effPCSA was +26.5 ± 5.7% greater during MVC than at rest. Similar moderate correlations existed for MVT and effPCSA at rest (r = 0.519, P = 0.047) and for MVT and effPCSA during MVC (r = 0.530, P = 0.042). Substantial changes in QF architecture (Lf, θp) and effPCSA occur in a curvilinear manner with relative torque production. The effPCSA during MVC was no more strongly associated with MVT than effPCSA measured at rest, which implies that resting measurements of muscle size are suitable for characterizing the muscle size–strength relationship.

Purpose: This study aimed (i) to determine the reliability of two-dimensional ultrasonography for the assessment of biceps femoris long head (BFlh) architectural characteristics and (ii) to determine whether limbs with a history of strain injury in the BFlh display different architecture and eccentric strength compared to uninjured limbs. Methods: This case–control study (control [n = 20], injured group [n = 16], males) assessed the BFlh architecture at rest and during graded isometric contractions using two-dimensional ultrasonography. The control group were assessed three times (>24 h apart) to determine reliability. Previously injured individuals were evaluated once. Results: The assessment of BFlh architecture was highly reliable (intraclass correlations >0.90). Fascicle length (P < 0.001; d range = 0.67–1.34) and fascicle length relative to muscle thickness (P < 0.001; d range = 0.58–0.85) of the previously injured BFlh were significantly less than the contralateral uninjured BFlh at all intensities. Pennation angle of the previously injured BFlh was significantly greater (P < 0.001; d range = 0.62–0.88) than the contralateral uninjured BFlh at all intensities. Eccentric strength in the previously injured limb was significantly lower than that in the contralateral limb (−15.4%; −52.5 N, 95% confidence interval = −76.2 to −28.4; P < 0.001, d = 0.56). Conclusions: These data indicate that two-dimensional ultrasonography is reliable for assessing BFlh architecture at rest and during graded isometric contractions. Fascicle length, fascicle length relative to muscle thickness, and pennation angle are significantly different in previously injured BFlh compared to an uninjured contralateral BFlh. Eccentric strength of the previously injured limb is also significantly lower than that of the uninjured contralateral limb. These findings have implications for rehabilitation and injury prevention practices, which should consider altered architectural characteristics.

Bulletin

International audience; PurposeWe investigated the effects of plantarflexor static stretching of different intensities on the medial gastrocnemius (GAS) shear elastic modulus, GAS fascicle length and ankle passive torque–angle responses during and after stretching.MethodsParticipants performed three stretching sessions of different intensities: 40 % (R40) of maximal dorsiflexion range of motion (ROM), 60 % (R60) of ROM, and 80 % (R80) of ROM. Each stretching lasted 10 min. The GAS architecture, GAS shear elastic modulus, ankle passive torque–angle, and muscle activity were assessed before, during, and after the stretching.ResultsThe absolute and relative (i.e., normalized to the static stretching start value) GAS shear elastic modulus relaxation varied across stretching intensities. The absolute passive torque relaxation varied across intensities (p < 0.05) but not when normalized to the stretching start value. No significant changes were observed in GAS fascicle length during the stretching (p = 0.93). After stretching, passive torque at a given angle was significantly decreased for R60 [−0.99 ± 0.59 Nm (−6.5 ± 3.8 %), p < 0.001] and R80 [−1.05 ± 1.12 Nm (−6.8 ± 6.3 %), p = 0.004], and GAS shear elastic modulus decreased only for the R80 [−9.3 ± 7.2 kPa (−14.1 %), p = 0.003]. No significant correlations were found between the magnitude of relaxation during stretching and post-stretching effect in the GAS shear elastic modulus or ankle passive torque variables. No significant relation was found between the shear elastic modulus and the ankle passive torque responses during and after stretching.ConclusionThe effects of stretching on joint passive torque do not reflect changes in the medial gastrocnemius shear elastic modulus, and these responses to stretching depend on its intensity.

BACKGROUND: Muscle mass, strength, and power are known determinants of mobility in older adults but there is limited knowledge on the influence of muscle architecture or tendon properties on mobility. The purpose of this study was to examine the relationship between mobility and plantarflexor muscle-tendon properties in healthy older adults.METHODS: A total of 52 subjects (age 70-81 years) were measured for 6-minute walk test (6MWT), timed "up and go"-test (TUG), isometric plantarflexion strength, Achilles tendon stiffness, triceps surae muscle architecture, lower extremity lean mass, isometric leg extension strength, and leg extension power. Partial correlations and multivariate regression models adjusted for sex, age, body mass, and height were used to examine the relationship between mobility (6MWT and TUG) and lower limb muscle-tendon properties.RESULTS: Multivariate regression models revealed that Achilles tendon stiffness (p = .020), plantarflexion strength (p = .022), and medial gastrocnemius fascicle length (p = .046) were independently associated with 6MWT. Plantarflexion strength (p = .037) and soleus fascicle length (p = .031) were independently associated with TUG.CONCLUSIONS: Plantarflexor muscle-tendon properties were associated with mobility in older adults independent of lower extremity lean mass, leg extension strength, or power. Plantarflexion strength was a stronger predictor of mobility than leg extension strength or power. The novel finding of this study was that muscle architecture and tendon properties explained interindividual differences in mobility. This study highlights the importance of the plantarflexors for mobility in older adults and provides understanding of possible mechanisms of age-related decline in mobility.

Purpose: To investigate differences in neuromuscular factors between elite and nonelite players and to establish which factors underpin direction-specific unilateral jump performance. Methods: Elite (n = 23; age, 18.1 [1.0] y; body mass index, 23.1 [1.8] kg·m−2) and nonelite (n = 20; age, 22.3 [2.7] y; body mass index, 23.8 [1.8] kg·m−2) soccer players performed 3 unilateral countermovement jumps (CMJs) on a force platform in the vertical, horizontal-forward, and medial directions. Knee extension isometric maximum voluntary contraction torque was assessed using isokinetic dynamometry. Vastus lateralis fascicle length, angle of pennation, quadriceps femoris muscle volume (Mvol), and physiological cross-sectional area (PCSA) were assessed using ultrasonography. Vastus lateralis activation was assessed using electromyography. Results: Elite soccer players presented greater knee extensor isometric maximum voluntary contraction torque (365.7 [66.6] vs 320.1 [62.6] N·m; P = .045), Mvol (2853 [508] vs 2429 [232] cm3; P = .001), and PCSA (227 [42] vs 193 [25] cm2; P = .003) than nonelite. In both cohorts, unilateral vertical and unilateral medial CMJ performance correlated with Mvol and PCSA (r ≥ .310, P ≤ .043). In elite soccer players, unilateral vertical and unilateral medial CMJ performance correlated with upward phase vastus lateralis activation and angle of pennation (r ≥ .478, P ≤ .028). Unilateral horizontal-forward CMJ peak vertical power did not correlate with any measure of muscle size or activation but correlated inversely with angle of pennation (r = −.413, P = .037). Conclusions: While larger and stronger quadriceps differentiated elite from nonelite players, relationships between neuromuscular factors and unilateral jump performance were shown to be direction-specific. These findings support a notion that improving direction-specific muscular power in soccer requires improving a distinct neuromuscular profile.

The developmental goal of 3D ultrasound imaging (3DUS) is to engineer a modality to perform 3D morphological ultrasound analysis of human muscles. 3DUS images are constructed from calibrated freehand 2D B-mode ultrasound images, which are positioned into a voxel array. Ultrasound (US) imaging allows quantification of muscle size, fascicle length, and angle of pennation. These morphological variables are important determinants of muscle force and length range of force exertion. The presented protocol describes an approach to determine volume and fascicle length of m. vastus lateralis and m. gastrocnemius medialis. 3DUS facilitates standardization using 3D anatomical references. This approach provides a fast and cost-effective approach for quantifying 3D morphology in skeletal muscles. In healthcare and sports, information on the morphometry of muscles is very valuable in diagnostics and/or follow-up evaluations after treatment or training.

The purpose of the present study was to evaluate active muscle stiffness with the stretch reflex according to changes (in 110-ms period after stretching) in torque and fascicle length during slower angular velocity (peak angular velocity of 100 deg·s−1) in comparison with active muscle stiffness without the stretch reflex (in 60-ms period after stretching) during slower and faster (peak angular velocity of 250 deg·s−1) angular velocities. Active muscle stiffness in the medial gastrocnemius muscle was calculated according to changes in estimated muscle force and fascicle length with slower and faster stretching during submaximal isometric contractions (10–90% maximal voluntary contractions). Active muscle stiffness significantly increased for both angular velocities and analyzed periods as torque levels exerted became higher. The effects of angular velocities and the interaction between angular velocities and torque levels were not significantly different between 250 deg·s−1 (in 60-ms period after stretching) and 100 deg·s−1 (in 110-ms period after stretching) conditions. The effects of the analyzed periods and the interaction between analyzed periods and torque levels were not significantly different between the analyzed periods (60-ms and 110-ms periods after stretching) for the 100 deg·s−1 condition. Furthermore, active muscle stiffness measured during the same angular velocity had significant correlations between those calculated in the different analyzed periods, whereas those under 250 deg·s−1 (60-ms period after stretching) did not correlate with those under 100 deg·s−1 (110-ms period after stretching). These results suggest that active muscle stiffness is not influenced by the stretch reflex.

International audience; While muscle contraction in voluntary efforts has been widely investigated, little is known about contraction during neuromuscular electrical stimulation (NMES). The aim of this study was to quantify in vivo muscle architecture of agonist and antagonist muscles at the ankle joint during NMES. Muscle fascicle lengths and pennation angles of the tibialis anterior (TA) and lateral gastrocnemius muscles were assessed via ultrasonography in 8 healthy young males. Measures were obtained during maximal NMES and torque-matched voluntary dorsiflexion contractions. In the TA, NMES induced a shorter fascicle length (67.2 ± 8.1 mm vs 74.6 ± 11.4 mm; p = 0.04) and a greater pennation angle (11.0 ± 2.4° vs 9.3 ± 2.5°; p = 0.03) compared with voluntary torque-matched dorsiflexion contractions. Architectural responses in the antagonist lateral gastrocnemius muscle did not significantly differ from rest or between voluntary and electrically induced contractions (p > 0.05). Contraction of the antagonist muscle was not a contributing factor to a greater fascicle shortening and increased pennation angle in the TA during NMES. TA architectural response during NMES likely arose from the contribution of muscle synergists during voluntary contractions coupled with a potentially localized contractile activity under the stimulation electrodes during NMES induced contractions.

The purpose of this study was to quantify the relative changes in fascicle (FL) and muscle-tendon unit (LMTU) length of the long head of the biceps femoris (BFlh) at different combinations of hip and knee joint positions. Fourteen participants performed passive knee extension trials from 0°, 45° and 90° of hip flexion. FL, LMTU, pennation angle (PA) and effective FL (FL multiplied by the cosine of the PA) of the BFlh were quantified using ultrasonography (US). Three-way analysis of variance designs indicated that at each hip angle, FL and LMTU increased and PA decreased from 90° to 0° of knee flexion. Increasing hip flexion angle from 0° to 90° led to a higher FL and LMTU and a lower PA (p .05). The average lengthening of the LMTU and effective FL was 28.00 ± 1.82% and 85.88 ± 21.92%, respectively. The average effective FL change accounted for 51.36 ± 7.39% of LMTU change. The relationship between effective FL and LMTU was almost linear with a slope equal to 0.49 ± 0.06 (r

Though the plasticity of human tendons is well explored in adults, it is still unknown how superimposed mechanical loading by means of athletic training affects the properties of tendons during maturation. Due to the increased responsiveness of muscle to mechanical loading, adolescence is an important phase to investigate the effects of training on the mechanical properties of tendons. Hence, in the present study we compared vastus lateralis (VL) architecture, muscle strength of the knee extensor muscles and patellar tendon mechanical properties of male and female adolescent elite athletes to untrained boys and girls. Twenty-one adolescent volleyball athletes (A; 16.7 ± 1 years; 12 boys, 9 girls) and 24 similar-aged controls (C; 16.7 ± 1 years; 12 boys and girls, respectively) performed maximum isometric contractions on a dynamometer for the assessment of muscle strength and, by integrating ultrasound imaging, patellar tendon mechanical properties. Respective joint moments were calculated using an inverse dynamics approach and an electromyography-based estimation of antagonistic contribution. Additionally, the VL pennation angle, fascicle length and muscle-thickness were determined in the inactive state by means of ultrasound. Adolescent athletes produced significantly greater knee extension moments (normalized to body mass) compared to controls (A: 4.23 ± 0.80 Nm/kg, C: 3.57 ± 0.67 Nm/kg; p = 0.004), and showed greater VL thickness and pennation angle (+38% and +27%; p < 0.001). Tendon stiffness (normalized to rest length) was also significantly higher in athletes (A: 86.0 ± 27.1 kN/strain, C: 70.2 ± 18.8 kN/strain; p = 0.04), yet less pronounced compared to tendon force (A: 5785 ± 1146 N, C: 4335 ± 1015 N; p < 0.001), which resulted in higher levels of tendon strain during maximum contractions in athletes (A: 8.0 ± 1.9%, C: 6.4 ± 1.8%; p = 0.008). We conclude that athletic volleyball training provides a more efficient stimulus for muscle compared to tendon adaptation, which results in an increased demand placed upon the tendon by the working muscle in adolescent volleyball athletes. Besides implications for sport performance, these findings might have important consequences for the risk of tendon overuse injury.

Purpose: To assess in-season alterations of biceps femoris long head (BFlh) fascicle length in elite Australian footballers with and without a history of unilateral hamstring strain injury (HSI) in the past 12 months. Methods: Thirty elite Australian football players were recruited. Twelve had a history of unilateral HSI. Eighteen had no HSI history. All had their BFlh architecture assessed at approximately monthly intervals, six times across a competitive season. Results: The previously injured limb's BFlh fascicles increased from the start of the season and peaked at week 5. Fascicle length gradually decreased until the end of the season, where they were shortest. The contralateral uninjured limb's fascicles were the longest when assessed at week 5 and showed a reduction in-season where weeks 17 and 23 were shorter than week 1. Control group fascicles were longest at week 5 and reduced in-season. The previously injured limb's BFlh fascicles were shorter than the control group at all weeks and the contralateral uninjured limb at week 5. Compared with the control group, the contralateral uninjured limb had shorter fascicles from weeks 9 to 23. Conclusions: Athletes with a history of HSI end the season with shorter fascicles than they start. Limbs without a history of HSI display similar BFlh fascicle lengths at the end of the season as they begin with. All athletes increase fascicle length at the beginning of the season; however, the extent of the increase differed based on history of HSI. These findings show that a HSI history may influence structural adaptation of the BFlh in-season.

Compared to pure isometric contractions, isometric muscle force at a given length is larger when the eccentric contraction is conducted before the isometric contraction. This phenomenon is widely known as residual force enhancement, and has been confirmed consistently in isolated muscle experiments. The purpose of this study was to confirm whether residual force enhancement also occurs in human plantar flexors and to examine its joint angle dependence. Eleven men participated in this study. Isometric joint torque was measured in a Control trial (pure isometric contraction) and Residual force enhancement (RFE) trial (isometric contraction after eccentric contraction) at plantar flexion 0° (Short condition) and dorsiflexion 15° (Long condition). Fascicle length and pennation angle of the medial gastrocnemius were measured simultaneously to evaluate the influence of architectural parameters on isometric joint torque. Isometric joint torque observed in the Short condition was not significantly different between the Control and RFE trials (Control: 42.9 ± 8.0 Nm, RFE: 45.1 ± 8.4 Nm) (p = 0.200). In contrast, significant differences in isometric joint torque were observed in the Long condition between Control and RFE trials (Control: 40.5 ± 9.3 Nm, RFE: 47.1 ± 10.5 Nm) (p = 0.001). Fascicle length and pennation angle were not different between Control and RFE trials in the Short and Long conditions. Isometric joint torque was larger when eccentric contraction was conducted before isometric contraction while architectural differences were not observed, indicating that residual force enhancement occurs in human plantar flexors. However, the influence of residual force enhancement may be limited in dorsiflexed positions because the magnitude of residual force enhancement is considered to be prominent in the descending limb (long muscle length condition) and small in the ascending limb (short muscle length condition) where human plantar flexors operate in plantar flexed positions.

Summary Materials suitable for anatomical research of raptorial birds are rare. Bird-eating raptors show distinct inter-sexual differences in body size and parental roles. The large females catch larger prey and prepare small morsels to feed their young using their hooked beaks. Here, we investigated the architectural properties of different jaw muscles of the Japanese sparrowhawk (Accipiter gularis) and examined whether there is sexual dimorphism in their architectural design. The results showed that musculus depressor mandibulae, the opener of the lower jaw, was characterized by relatively long fascicle length, whereas musculus pterygoideus was characterized by its larger mass and physiological cross-sectional area (PCSA) in both sexes. Females have the potential capacity to produce rapid and strong bites by their significantly longer fascicle length of M. depressor mandibulae and larger mass and PCSA of M. pterygoideus. For body size-matched gender, jaw muscles of males had fibres of relatively longer length than females, enabling greater velocity and excursion. Architectural characteristics of jaw muscles, together with the absolute dimorphism (the fascicle length of M. depressor mandibulae, the muscle mass and PCSA of M. pterygoideus) and relative dimorphism in the muscle mass of M. pterygoideus, reflect dietary difference and asymmetric parental roles between the sexes.

Isometric muscle force attained during isometric contractions decreases after active shortening compared to that attained during purely isometric contractions. This phenomenon is called residual force depression. The aim of this study was to examine whether residual force depression occurs in human plantar flexors in both plantar flexed and dorsiflexed region. In addition, the magnitude of fascicle shortening was evaluated because not only muscle force but also fascicle shortening during active shortening are considered to affect force depression. Eleven male subjects were recruited. All muscle contractions were evoked by muscle belly-electrical stimulation. In the reference trials, isometric plantar flexion (PF) was performed at 0° and 15° of PF. In the residual force depression trials, the following two contractions were conducted: 1) muscles were activated isometrically at 15° of dorsiflexion, then actively shortened to 0° of PF (long condition) and 2) muscles were activated isometrically at 0° of PF, then actively shortened to 15° of PF (short condition). Isometric joint torque obtained 4.9 seconds after the onset of contraction was compared between the reference and residual force enhancement trials at the same joint angle to calculate the magnitude of residual force depression. At the same time point, fascicle length and pennation angle were obtained from ultrasonographic images to examine whether the muscle architecture affected residual force depression. As a result, residual force depression was confirmed in both the long and short length conditions (long: 87.1 ± 9.1%, short: 92.1 ± 7.8%) while the magnitude was not different (p = 0.182). The fascicle length and pennation angle were not different between the reference and residual force depression trials (p = 0.291–0.906). These results indicate that residual force depression occurs in the physiological range of motion in the human plantar flexors, and this phenomenon is not related to muscle architecture. In addition, joint angle dependence of the residual force depression was not observed between long and short muscle length conditions.

Background: The architectural and morphological adaptations of the hamstrings in response to training with different exercises have not been explored. Purpose: To evaluate changes in biceps femoris long head ( BFLH ) fascicle length and hamstring muscle size following 10-weeks of Nordic hamstring exercise ( NHE ) or hip extension ( HE ) training. Methods: 30 recreationally active male athletes ( age, 22.0±3.6 years; height, 180.4±7 cm; weight, 80.8±11.1 kg ) were allocated to 1 of 3 groups: ( 1 ) HE training ( n=10 ), NHE training ( n=10 ), or no training ( control, CON ) ( n=10 ). BFLH fascicle length was assessed before, during ( Week 5 ) and after the intervention with a two-dimensional ultrasound. Hamstring muscle size was determined before and after training via MRI. Results: Compared with baseline, BFLH fascicles were lengthened in the NHE and HE groups at mid-training ( d=1.12–1.39, p < 0.001 ) and post-training ( d=1.77–2.17, p < 0.001 ) and these changes did not differ significantly between exercises ( d=0.49–0.80, p=0.279–0.976 ). BFLHvolume increased more for the HE than the NHE ( d=1.03, p=0.037 ) and CON ( d=2.24, p < 0.001 ) groups. Compared with the CON group, both exercises induced significant increases in semitendinosus volume ( d=2.16–2.50, ≤0.002 ) and these increases were not significantly different ( d=0.69, p=0.239 ). Conclusion: NHE and HE training both stimulate significant increases in BFLH fascicle length; however, HE training may be more effective for promoting hypertrophy in the BFLH.

Skeletal muscles are essential to the gender-specific characteristics of human movements. Sonomyography, a new signal for quantifying muscle activation, is of great benefit to understand muscle function through monitoring the real-time muscle architectural changes. The purpose of this pilot study was to investigate gender differences in the architectural changes of gastronomies muscle and tendon by using sonomyography during performing two-legged calf raising exercises. A motion analysis system was developed to extract sonomyography from ultrasound images together with kinematic and kinetic measurements. Tiny fascicle length changes among seven male subjects were observed at the initial part of calf raising, whereas the fascicle of seven female subjects shortened immediately. This result suggested that men would generate higher mechanical power output of plantar flexors to regulate their heavier body mass. In addition, the larger regression coefficient between the fascicle length and muscle force for the male subjects implied that higher muscle stiffness for the men was required in demand of maintaining their heavier body economically. The findings from the current study suggested that the body mass might play a factor in the gender difference in structural changes of muscle and tendon during motion. The sonomyography may provide valuable information in the understanding of the gender difference in human movements.

Introduction A precise quantitative measurement of skeletal muscle mass is fundamental for diagnosing sarcopenia in older individuals. The current techniques of assessment, including dual-energy x-ray absorptiometry (DXA), bioimpedance analysis (BIA), and magnetic resonance imaging (MRI) are either difficult to perform in everyday clinical practice or biased by concurrent clinical confounders. B-mode muscle ultrasound can be helpful in assessing muscle mass and architecture, and thus possibly useful for diagnosing or screening sarcopenia. Methods A literature search of published articles on muscle ultrasound and sarcopenia in older individuals as of July 31, 2016, was made on PubMed and Scopus. Manual search and cross-referencing from reviews and original articles was also performed. Results Most of the existing studies were carried out on healthy well-fit subjects, with a low prevalence of sarcopenia. The main parameters that can be assessed through muscle ultrasound are muscle thickness, cross-sectional area, echo intensity, and, for pennate muscles, fascicle length and pennation angle. In older subjects, all these parameters show some degree of alteration compared to young adults, particularly in lower limb muscles with antigravitary function, such as the quadriceps femoris and gastrocnemius medialis. Each of these parameters may be theoretically useful for detecting the loss of muscle mass and functionality in geriatric patients. They are also poorly influenced by the presence of acute and chronic diseases and fluid balance, unlike DXA and BIA, but a high degree of standardization in ultrasound protocols is necessary. Frontier applications of ultrasound in the assessment of sarcopenia may include contrast-enhanced and diaphragm ultrasound. Conclusions The current literature does not allow to make conclusive recommendations about the use of muscle ultrasound in geriatric practice. However, this technique is very promising, and further studies should validate its applications in the context of sarcopenia assessment.

PurposeVarious strategies for improving reliability of fascicle identification on ultrasound images are used in practice, yet these strategies are untested for effectiveness. Studies suggest that the largest part of differences between fascicle lengths on one image are attributed to the error on the initial image. In this study, we compared reliability results between different strategies.MethodsStatic single-image recordings and image sequence recordings during passive ankle rotations of the medial gastrocnemius were collected. Images were tracked by three different raters. We compared results from uninformed fascicle identification (UFI) and results with information from dynamic length changes, or data-informed tracking (DIT). A second test compared tracking of image sequences of either fascicle shortening (initial-long condition) or fascicle lengthening (initial-short condition).ResultsIntra-class correlations (ICC) were higher for the DIT compared to the UFI, yet yielded similar standard error of measurement (SEM) values. Between the initial-long and initial-short conditions, similar ICC values, coefficients of multiple determination, mean squared errors, offset-corrected mean squared errors and fascicle length change values were found for the DIT, yet with higher SEM values and greater absolute fascicle length differences between raters on the first image in the initial-long condition and on the final image in the initial-short condition.ConclusionsDIT improves reliability of fascicle length measurements, without lower SEM values. Fascicle length on the initial image has no effect on subsequent tracking results. Fascicles on ultrasound images should be identified by a single rater and care should be taken when comparing absolute fascicle lengths between studies.

ADAM J. WELLS, MS, DAVID H. FUKUDA, PhD, JAY R. HOFFMAN, PhD, ADAM M. GONZALEZ, MEd,ADAM R. JAJTNER, MS, JEREMY R. TOWNSEND, MS, GERALD T. MANGINE, MEd, MAREN S. FRAGALA, PhD, andJEFFREY R. STOUT, PhDInstitute of Exercise Physiology and Wellness, University of Central Florida (UCF), 4000 Central Florida Blvd Orlando, Florida, 32816,USAAccepted 21 February 2014ABSTRACT: Introduction: Variations in transverse point of mea-sure on the vastus lateralis (VL) may significantly affect therelationship between structure and function. The purpose of thisstudy was to compare changes in muscle architecture at 2commonly used points of measure (VL0 and VL5). Methods:Maximal strength (1-repetition maximum [1RM] barbell squat)and muscle architecture were assessed PRE and POST 15weeks of periodized resistance training. VL0 was 50% of thestraight line distance between the greater trochanter and lateralepicondyle of the femur. VL5 was 5cm medial to VL0. Results:Increases in 1RM strength (3.762.4 kg; P50.004) wereobserved. Changes in muscle thickness (MT) at VL5 were sig-nificantly greater than at VL0 (P50.006). Changes in strengthcorrelated with changes in muscle architecture at VL0 only (MT:r50.561; fascicle length: r50.503). Conclusions: Changes inmuscle architecture appear to occur in a non-homogeneousmanner.

The purpose of this study was to investigate the relationship between 25-m sprint front crawl swimming performance and muscle fascicle length in young male swimmers. 23 swimmers were selected and divided into two groups according to their best records of 25-m sprint performance: 14.6-15.7 sec (S1, n = 11) and 15.8-17 sec (S2, n = 12). Muscle thickness and pennation angle of Biceps Brachii (BB; only muscle thickness), Triceps Brachii (TB), Vastus Lateralis (VL), Gastrocnemius Medialis (GM) and Lateralis (GL) muscles were measured by B-mode ultrasonography, and fascicle length was estimated. Although, there was no significant differences between groups in anthropometrical parameter as standing height, body mass, arm length, thigh length and leg length (p < 0.001), however, S1 significantly had a greater muscle thickness in VL, GL, and TB muscles (p < 0.05). Pennation angle only in TB was significantly smaller in S1 (p < 0.05). S1 in VL, GL, and TB muscles significantly had greater absolute fascicle length and in VL and TB muscles had relatively (relative to limb length) greater fascicle length (p < 0.05). Moreover, there was a significant relationship between sprint swimming time and absolute and relative fascicle length in VL (absolute: r = -0.49 and relative: r = -0.43, both p < 0.05) and GL (absolute: r = -0.47 and relative: r = -0.42, both p < 0.05). Potentially, it seems that fascicle geometry developed in muscles of faster young swimmers to help them to perform their high speed movement.

In this study we investigated the relationships between muscle-tendon parameters and average/peak values of velocity, force and power in sprint running focusing on the acceleration phase. Eighteen male sprinters (100 m PB: 10.66±0.51 s) participated to the study. Instantaneous values of horizontal velocity (v) were recorded by means of a radar and instantaneous values of force (F) and power (P) were calculated based on these data. Muscle thickness, fascicle length and pennation angle of knee extensors and plantar flexors, as well as Achilles tendon length and CSA, were measured by means of ultrasonography. In the first 20 m of the sprint average and peak speed were 6.31±0.59 and 8.88±0.98 m·s-1, respectively; force was highest at the start of the sprint (Fpeak = 10.02±1.43 N·kg-1) and power peaked about 1 s after the start (26.64±5.99 W·kg-1). Muscle-tendon parameters showed stronger correlations with peak values of power (R range: 0.81-0.92), force (R range: 0.56-0.84) and speed (R range: 0.53-0.85) than with average values of velocity over the 20 m distance (R range: 0.41-0.61) (R 0.71 = P < .001). These data underline that the influence of muscle tendon parameters on sprint performance could be better appreciated when peak values of power can be calculated rather than by considering the simple measure of average velocity (e.g. distance/time).

Objective: The purpose of this study was to investigate the effect of Nordic hamstring exercise group withNordic hamstring exercise group that was doing resistance movements together on the hamstring muscle bycomparing the measurement of fascicle length(FL), muscle thickness(MT), of hamstring muscle and the sizeof the pennation angle(PA) after a certain period of intervention.Method: This study was conducted by recruiting 20 physically healthy adult males and 21 females whoagreed to participate in this study after the preliminary study. The training group(TG) that did the Nordichamstring exercise and resistance movement together(RNHE) and the controlled group(CG) that did Nordichamstring exercise(NHE) were measured for hamstring muscle 6 weeks after exercise intervention accordingto the predetermined protocol for 5 weeks and after 3 weeks of tracing observation, they were measuredto compare the prognosis after 9 weeks. All measurements in the experiment were conducted in repeatedmeasured ANOVA, and t-test was used to compare the two groups.Findings: The result of the experiment showed statistically significant differences in the FL, MT, ofhamstring muscle and the size of the PA in both groups. In addition, the FL of hamstring muscle and the sizeof the PA showed significant differences in 6 weeks and 9 weeks.Improvements: In conclusion, it was confirmed that applying RNHE has effect in the MT and FL ofhamstring muscle and the size of the PA, and this can be an even more effective exercise intervention thatcan lower the risk of hamstring injury when applied to athletes.