343 results on '"BUFFA R"'
Search Results
302. Identification of the endocrine cells detected by the monoclonal antibody HISL-19 in human tissues.
- Author
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Buffa R, Pelagi M, Siccardi AG, Curzio M, Yu JY, and Bordi C
- Subjects
- Adrenal Medulla cytology, Adrenal Medulla immunology, Chromogranin A, Chromogranins immunology, Digestive System cytology, Digestive System immunology, Endocrine Glands immunology, Epitopes immunology, Humans, Immunohistochemistry methods, Islets of Langerhans cytology, Islets of Langerhans immunology, Pituitary Gland cytology, Pituitary Gland immunology, Thyroid Gland cytology, Thyroid Gland immunology, Antibodies, Monoclonal immunology, Endocrine Glands cytology
- Abstract
The human endocrine cells reacting with the monoclonal antibody HISL-19 were identified with hormone antisera of proven specificity using a double immunostaining procedure. The epitope for HISL-19 was found in all types of pituitary cells except ACTH cells, in thyroid C cells, in all types of adrenal medullary and pancreatic islet cells and in somatostatin and pancreatic polypeptide cells of the gastrointestinal mucosa. No staining was found in parathyroid cells and in most gastrointestinal endocrine cells. Either paranuclear focal accumulation or diffuse cytoplasmic distribution of immunoreactive material were found. The spectrum of HISL-19 immunoreactive cells was found to be only in part complementary to that of cells immunoreactive for chromogranin A. Thus, it is concluded that the monoclonal antibody HISL-19 is a useful addition to other immunohistochemical markers for the detection of cells showing neuroendocrine features.
- Published
- 1990
303. Immunohistochemical detection of 28KDa calbindin in human tissues.
- Author
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Buffa R, Mare' P, Salvadore M, and Gini A
- Subjects
- Calbindins, Humans, Immunoenzyme Techniques, Organ Specificity, S100 Calcium Binding Protein G analysis
- Published
- 1990
- Full Text
- View/download PDF
304. Glucagon-, glicentin-, and pancreatic polypeptide-like immunoreativities in rectal carcinoids and related colorectal cells.
- Author
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Fiocca R, Capella C, Buffa R, Fontana R, Solcia E, Hage E, Chance RE, and Moody AJ
- Subjects
- Adult, Antigen-Antibody Reactions, Colon, Sigmoid cytology, Colon, Sigmoid immunology, Colon, Sigmoid ultrastructure, Humans, Intestinal Mucosa ultrastructure, Male, Middle Aged, Polyps immunology, Rectal Neoplasms immunology, Rectum cytology, Rectum ultrastructure, Glucagon immunology, Pancreatic Polypeptide immunology, Polyps ultrastructure, Rectal Neoplasms ultrastructure
- Abstract
Three nonargentaffin rectal carcinoids have been investigated immunohistochemically. In one case most tumor cells reacted with antiglucagon sera as well as with antiglicentin, antibovine pancreatic polypeptide (BPP), and antihuman pancreatic polypeptide (HPP) sera; they were identified ultrastructurally as L cells. Another case showed glucagon-, glicentin-, and BPP-immunoreactive cells but lacked HPP immunoreactivity. In the third case glucagon- and glicentin-immunoreactive cells were well represented, while PP immunoreactivities were scarce. Parallel investigations of human rectal and sigmoid mucosa showed numerous cells reacting with glucagon, glicentin, and BPP antisera, most of which lacked HPP immunoreactivity. Cells reacting with glucagon and glicentin antisera, while lacking PP immunoreactivities, were also found. Thus, both tumor and nontumor cells produce glucagonlike immunoreactive (GLI) peptides--one of which may be glicentin or a related molecule--as well as PP-related sequences, although differing histochemically and ultrastructurally from glucagon or PP cells of the human pancreas. It is concluded that nonargentaffin rectal carcinoids are histogenetically linked to nonargentaffin endocrine cells of the human rectum.
- Published
- 1980
305. Parafollicular cells of rabbit thryoid store both calcitonin and somatostatin and resemble gut D cells ultrastructurally.
- Author
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Buffa R, Chayvialle JA, Fontana P, Usellini L, Capella C, and Solcia E
- Subjects
- Animals, Dogs, Guinea Pigs, Histocytochemistry, Humans, Immunochemistry, Organ Specificity, Rabbits, Species Specificity, Thyroid Gland cytology, Thyroid Gland metabolism, Calcitonin metabolism, Pylorus ultrastructure, Somatostatin metabolism, Thyroid Gland ultrastructure
- Abstract
Both calcitonin and somatostatin have been detected immunohistochemically in rabbit parafollicular cells; only calcitonin has been found in the same cells of the dog, guinea-pig and man. Large amounts of a peptide radioimmunochemically identical with synthetic somatostatin have been detected in extracts of rabbit thyroid. The ultrastructural and staining features of rabbit parafollicular cells differ from those of parafollicular cells in other species, while resembling in part those of somatostatin D cells scattered in the rabbit stomach.
- Published
- 1979
- Full Text
- View/download PDF
306. Antigenic markers of neuroendocrine tumors: their diagnostic and prognostic value.
- Author
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Solcia E, Capella C, Buffa R, Tenti P, Rindi G, and Cornaggia M
- Subjects
- Endocrine System Diseases pathology, Humans, Neoplasms pathology, Prognosis, Antigens, Neoplasm analysis, Endocrine System Diseases diagnosis, Neoplasms diagnosis
- Published
- 1986
307. Formaldehyde-ozone-induced fluorescence in gastrin-producing tumours.
- Author
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Larsson LI, Sundler F, Grimelius L, Håkanson R, Buffa R, and Solcia E
- Subjects
- Adult, Aged, Duodenal Neoplasms metabolism, Duodenal Neoplasms pathology, Female, Fluorescence, Formaldehyde, Gastrins metabolism, Glucagon metabolism, Humans, Insulin metabolism, Insulin Secretion, Male, Middle Aged, Ozone, Pancreatic Neoplasms metabolism, Stomach Neoplasms metabolism, Stomach Neoplasms pathology, Tryptophan, Zollinger-Ellison Syndrome metabolism, Zollinger-Ellison Syndrome pathology, Gastrointestinal Neoplasms pathology, Pancreatic Neoplasms pathology
- Abstract
Material from eight peptide hormone-secreting tumours, extirpated from the pancreas or from the antrum-duodenum region, was examined. Four of the patients had the clinical manifestations of the Zollinger-Ellison syndrome, two showed the features of an insulin-secreting tumour and one had a glucagonoma. Gastrin-producing cells, identified by immunohistochemistry, were found in five of the tumours. These cells displayed a varying degree of formaldehyde-ozone-induced fluorescence. This agrees with previous observations on the gastrin cell of human antral and duodenal mucosa. From model experiments, formaldehyde-ozone-induced fluorescence is thought to reflect the presence of peptides having tryptophan in the NH2-terminal position. The nature of this peptide in gastrin-producing cells is unknown.
- Published
- 1975
- Full Text
- View/download PDF
308. Multiple endocrine cell types in thyroid medullary carcinoma. Evidence for calcitonin, somatostatin, ACTH, 5HT and small granule cells.
- Author
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Capella C, Bordi C, Monga G, Buffa R, Fontana P, Bonfanti S, Bussolati G, and Solcia E
- Subjects
- Adult, Carcinoma analysis, Carcinoma ultrastructure, Child, Female, Humans, Male, Middle Aged, Thyroid Neoplasms analysis, Thyroid Neoplasms ultrastructure, Adrenocorticotropic Hormone analysis, Calcitonin analysis, Carcinoma pathology, Serotonin analysis, Somatostatin analysis, Thyroid Neoplasms pathology
- Abstract
10 cases of thyroid medullary carcinoma (TMC) have been studied ultrastructurally and histochemically. Well differentiated calcitonin-producing C cells were present in all tumours, being prevalent in 9 cases. 5-Hydroxytryptamine (5HT) storing cells were found in two cases, somatostatin immunoreactive cells in at least 5 cases and ACTH-immunoreactive cells in 4 cases. Ultrastructurally, at least 3 types of apparently non-C cells were observed. Type 1 cells with large, poorly osmiophilic granules resembling those of gastroenteropancreatic D cells, were present in 6 cases; they appeared to correlate well with somatostatin immunoreactive cells. Type 2 cells with large osmiophilic granules were found in 5 cases; they resembled ACTH-MSH cells of the human pituitary and may correspond to the ACTH-immunoreactive cells of light microscopy. Type 3 cells with small granules and an unknown function were found in 6 cases, always in scarce number. It is concluded that TMC, although mainly made up of C cells, usually contains large proportions of other endocrine cell types.
- Published
- 1978
- Full Text
- View/download PDF
309. Types of endocrine cells in the human colon and rectum.
- Author
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Buffa R, Capella C, Fontana P, Usellini L, and Solcia E
- Subjects
- Cytoplasmic Granules ultrastructure, Enterochromaffin Cells ultrastructure, Glucagon metabolism, Humans, Pancreatic Polypeptide metabolism, Serotonin metabolism, Somatostatin metabolism, Chromaffin System analysis, Colon cytology, Endocrine Glands cytology, Enterochromaffin Cells analysis, Rectum cytology
- Abstract
At least four types of endocrine-like cells have been detected histochemically in the mucosa of the human colon and rectum, i.e. argentaffin cells storing 5-hydroxytryptamine (5HT) and non-argentaffin cells reacting with glucagon, somatostatin and bovine pancreatic peptide (BPP) antibodies. Ultrastructurally, four main types and three rare types of endocrine-like cells have been identified. Among the former cells were: (1) argentaffin EC1 cells, known to store 5HT and substance P, (2) poorly argyrophil L cells, corresponding to the glucagon-immunoreactive cells storing enteroglucagon or glucagon-like immunoreactivity (GLl), (3) inconstantly argyrophil F-like cells, possibly corresponding to BPP-immunoreactive cells, and (4) fairly argyrophil H cells of unknown function. Rare D cells, corresponding to somatostatin cells, N cells, corresponding to neurotensin cells, and P cells, of unknown function, have been also found.
- Published
- 1978
- Full Text
- View/download PDF
310. The contribution of immunohistochemistry to the diagnosis of neuroendocrine tumors.
- Author
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Solcia E, Capella C, Buffa R, Usellini L, Fiocca R, Sessa F, and Tortora O
- Subjects
- Gastrointestinal Neoplasms analysis, Humans, Immunohistochemistry, Lung Neoplasms analysis, Neoplasms, Nerve Tissue analysis, Pancreatic Neoplasms analysis, Urogenital Neoplasms analysis, Neoplasms analysis, Neurosecretory Systems analysis
- Abstract
The use of specific secretory products, hormones, and neuroregulators as diagnostic tools for neuroendocrine tumors is illustrated. Results of extensive immunohistochemical and cytologic investigations are discussed in the light of other pathologic and clinical findings to serve as a basis for tumor classification and as a help in prognostic evaluation. Endocrine tumors of the pancreas, gut, lung and urogenital tract are dealt in some detail.
- Published
- 1984
311. The endocrine cells of the pancreas and related tumours. Ultrastructural study and classification.
- Author
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Capella C, Solcia E, Frigerio B, Buffa R, Usellini L, and Fontana P
- Subjects
- Animals, Cats, Cell Differentiation, Dogs, Gastrins, Glucagon, Guinea Pigs, Humans, Insulin, Islets of Langerhans ultrastructure, Microscopy, Electron, Pancreatic Neoplasms classification, Serotonin, Somatostatin, Swine, Adenoma, Islet Cell pathology, Pancreatic Neoplasms pathology
- Abstract
Up to seven endocrine cell types have been identified ultrastructurally in the pancreas, including glucagon A cells, insulin B cells, somatostatin D cells, pancreatic peptide F cells and 5-hydroxytryptamine EC cells. In addition, D1 cells, which have been proposed as the cell type producing VIP and possible P cells of unknown function are seen. Various patterns of endocrine cell differentiation have been found in 20 endocrine pancreatic tumours. Well and poorly differentiated B cells have been identified in 6 insulinomas, diagnostic G cells in 3 out of 7 gastrinomas, D1 and/or F cells in 7 diarrheogenic tumours. Moreover, cells apparently unrelated to the prevalent clinical syndrome have been noted in 8 of the 20 tumours. Granular non diagnostic cells (poorly diagnostic gastrin cells? D1 cells?) were particularly frequent in gastrinomas; agranular or poorly granular cells, either by "active" or "Stem cell" type, were present in nearly all tumours, particularly in diarrheogenic tumours, gastrinomas and malignant insulinomas. A cytological classification of pancreatic endocrine tumours is proposed.
- Published
- 1977
- Full Text
- View/download PDF
312. Immunoreactivity of hormonally-characterized human endocrine cells against three novel anti-human chromogranin B(B11 and B13) and chromogranin A (A11) monoclonal antibodies.
- Author
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Buffa R, Gini A, Pelagi M, Siccardi AG, Bisiani C, Zanini A, and Solcia E
- Subjects
- Chromogranin A, Chromogranin B, Chromogranins metabolism, Endocrine Glands metabolism, Humans, Immunohistochemistry, Antibodies, Monoclonal immunology, Chromogranins immunology, Endocrine Glands cytology, Nerve Tissue Proteins immunology
- Abstract
Two novel monoclonal antibodies, called B11 and B13, directed exclusively against human chromogranin B (CgB) and another antibody, A11, specific for human chromogranin A (CgA), were obtained by immunization of mice with chromaffin granules, the fusion of their splenocytes, the screening of hybridomas supernatants by ELISA and immunohistochemistry, and characterization of the antibodies by two-dimensional immunoblotting. The antibodies were used in immunohistochemical tests to investigate the distribution of CgA and CgB in hormonally-identified cells of the human endocrine system. The A11 antibody confirmed the occurrence of CgA in gut EC, ECL, gastrin, secretin and neurotensin cells, pancreatic A and PP cells, parathyroid chief cells, pituitary TSH and gonadotrope cells and adrenal medullary cells. Only a fraction of CgA-immunoreactive cells in the human gut and pancreas showed C-terminus arginine-glycinamide immunoreactivity, suggesting pancreastatin storage. Both CgB antibodies showed immunoreactivity in gastrin cells, intestinal (but not gastric) EC cells, pancreatic A and PP cells, pituitary TSH and gonadotrope cells and adrenal medullary cells. In addition, the B11 antibody stained thyroid C cells and the B13 antibody stained the Golgi area of pituitary GH cells. It is concluded that most CgB is stored in the same cells showing CgA, although some CgA-rich cells, like gastric EC and ECL cells. lacked B11 and B13 immunoreactivities and some CgA-poor cells, like human thyroid C cells, showed intense B11 immunostaining.
- Published
- 1989
- Full Text
- View/download PDF
313. Letter: Azo coupling of indole-reactive enterochromaffin cells.
- Author
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Solcia E and Buffa R
- Subjects
- Animals, Azo Compounds, Chromaffin System analysis, Colon cytology, Evaluation Studies as Topic, Glutaral, Guinea Pigs, Histocytochemistry, Humans, Indoles, Intestinal Mucosa cytology, Intestine, Large cytology, Leukocytes cytology, Methods, Mice, Organ Specificity, Rabbits, Serotonin analysis, Species Specificity, Staining and Labeling, Stomach cytology, Chromaffin System cytology
- Published
- 1974
- Full Text
- View/download PDF
314. Synaptophysin immunoreactivity and small clear vesicles in neuroendocrine cells and related tumours.
- Author
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Buffa R, Rindi G, Sessa F, Gini A, Capella C, Jahn R, Navone F, De Camilli P, and Solcia E
- Subjects
- Chromogranin A, Chromogranins analysis, Chromogranins immunology, Humans, Immunohistochemistry, Infant, Membrane Proteins immunology, Microscopy, Electron, Neoplasms ultrastructure, Neurosecretory Systems ultrastructure, Pancreas analysis, Pituitary Gland analysis, Synaptophysin, Cytoplasmic Granules analysis, Membrane Proteins analysis, Neoplasms analysis, Neurosecretory Systems analysis, Pituitary Gland ultrastructure
- Abstract
Synaptophysin (protein p38) immunoreactivity has been detected immunohistochemically in neuroendocrine cells of the human adrenal medulla, carotid body, skin, pituitary, thyroid, lung, pancreas and gastrointestinal mucosa as well as in 87 out of 93 neuroendocrine tumours investigated, including pheochromocytomas, chromaffin and non-chromaffin paragangliomas, ganglioneuromas, pituitary adenomas, thyroid medullary carcinomas, parathyroid adenomas, lung carcinoids and neuroendocrine carcinomas, pancreatic and gut endocrine tumours and cutaneous merkelomas. Parallel ultrastructural investigation of synaptophysin-reactive cells and tumours revealed the presence, in addition to dense-cored, secretory granules, of a population of pleomorphic, small, clear vesicles resembling synaptic vesicles of nerve terminals as well as the synaptophysin immunoreactive vesicles already described in rat adrenal medullary and pituitary cells. Synaptophysin immunoreactivity showed several differences in its distribution among tumour and non-tumour endocrine cells when compared to chromogranin A immunoreactivity, a well known marker of the core of endocrine granules. Synaptophysin represents a reliable general marker of neuroendocrine cells and tumours, which may be useful in diagnostic histopathology.
- Published
- 1987
- Full Text
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315. Chromogranins A and B and secretogranin II in hormonally identified endocrine cells of the gut and the pancreas.
- Author
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Buffa R, Marè P, Gini A, and Salvadore M
- Subjects
- Animals, Cats, Chromogranin A, Colon analysis, Dogs, Gastric Mucosa analysis, Guinea Pigs, Immunohistochemistry, Intestinal Mucosa analysis, Intestine, Small analysis, Tissue Distribution, Chromogranins analysis, Digestive System analysis, Endocrine Glands analysis, Nerve Tissue Proteins analysis, Pancreas analysis, Proteins analysis
- Abstract
Chromogranins A and B and secretogranin II have been localized in a wide spectrum of gastroenteropancreatic endocrine/paracrine cells. Chromogranin A immunoreactivity showed the widest distribution and was displayed by glucagon-, PP-, gastrin-, gastrin-CCK-, secretin-immunoreactive cells, the most intense stainings being peculiar of enterochromaffin cells. Chromogranin B immunoreactivity was detected in gastrin- and glucagon cells and in some enterochromaffin cells containing also chromogranin A. Secretogranin II was paired to chromogranin A in glucagon cells of pancreatic islets or occurred alone in glycentin/PP cells of colonic mucosa. Neither of the chromogranins nor secretogranin II have been so far detected in somatostatin-, GIP-, or motilin-immunoreactive cells. Chromogranin A but not chromogranin B or secretogranin II has been detected in the gastric argyrophilic ECL cells.
- Published
- 1988
316. Identification of the intestinal cell storing gastric inhibitory peptide.
- Author
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Buffa R, Polak JM, Pearse AG, Solcia E, Grimelius L, and Capella C
- Subjects
- Animals, Dogs, Duodenum analysis, Enterochromaffin Cells, Fluorescent Antibody Technique, Histocytochemistry, Humans, Intestinal Mucosa analysis, Intestinal Mucosa cytology, Intestine, Small cytology, Peptides analysis, Swine, Gastrointestinal Hormones analysis, Intestine, Small analysis
- Abstract
Small intestinal mucosal samples from man, pig and dog, were subjected to sequential or correlative silver impregnation techniques, applied to immunocytochemical preparations and at the ultrastructural level. The cell reacting with anti-GIP sera was identified as the ultrastructurally classified K cell and we propose that the term GIP cell be used in place of the latter. This cell can thus be recognized by its strong reactivity with the Sevier-Munger staining procedure, provided that the equally strongly reacting EC cell is excluded by virtue of its argentaffinity with the Masson technique.
- Published
- 1975
- Full Text
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317. The endocrine cells of the chicken proventriculus.
- Author
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Usellini L, Tenti P, Fiocca R, Capella C, Buffa R, Terenghi C, Polak JM, and Solcia E
- Subjects
- Animals, Bombesin metabolism, Chickens anatomy & histology, Enterochromaffin Cells cytology, Glucagon metabolism, Histocytochemistry, Proventriculus physiology, Proventriculus ultrastructure, Serotonin metabolism, Somatostatin metabolism, Gastrointestinal Hormones metabolism, Proventriculus cytology
- Abstract
The endocrine cells of the chicken proventriculus were investigated by selective staining techniques, immunohistochemistry and electron microscopy. The following endocrine cell types were identified: 1) Argyrophilic ECL-cells, of unknown function, were very numerous in the 21-day-old chick, but less numerous in the newborn chick; 2) somatostatin-producing D-cells; 3) GLI-cells producing glucagon-related peptides; 4) X-cells of unknown function; 5) BN-cells producing bombesin; and 6) relatively few 5-hydroxytryptamine-producing EC-cells. Each of these cell types show a distinct morphology, distribution and histochemical reactivity. With the exception of BN-cells, they resemble rather closely the corresponding endocrine cell types previously described in the oxyntic mucosa (EGL, D, X and EC cells) or in the intestinal mucosa (L-cells) of the mammalian gut.
- Published
- 1983
318. Argyrophil pituitary tumors showing TSH cells or small granule cells.
- Author
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Capella C, Usellini L, Frigerio B, Buffa R, Fontana P, and Solcia E
- Subjects
- Adenoma, Chromophobe ultrastructure, Adult, Aged, Female, Humans, Male, Microscopy, Electron, Middle Aged, Pituitary Neoplasms analysis, Pituitary Neoplasms ultrastructure, Pituitary Neoplasms pathology, Thyrotropin analysis
- Abstract
Among 74 histochemically and ultrastructurally studied pituitary adenomas, 12 apparently chromophobe tumors were characterized by the presence of numerous argyrophil cells. All these argyrophil adenomas failed to reveal presence of GH, prolactin or ACTH cells. Two tumors were found to consist of well granulated cells reacting intensely with anti-TSH antibodies and resembling TSH cells of the normal pituitary. The remaining argyrophil adenomas did not show TSH immunostaining and, with one exception, failed to react with an anti-HCG serum staining gonadotroph cells of human pituitary. They were composed of small, closely apposed cells with small compact or vesicular granules. These tumor cells seem to correspond to some small argyrophil cells found in non-neoplastic pituitary, which differ from TSH cells and from all other types of functionally identified adenohypophyseal cells.
- Published
- 1979
- Full Text
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319. Immunohistochemical localization of polypeptides in peripheral autonomic nerves using whole mount preparations.
- Author
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Costa M, Buffa R, Furness JB, and Solcia E
- Subjects
- Animals, Fixatives, Fluorescent Antibody Technique, Formaldehyde, Guinea Pigs, Histocytochemistry, Immunoenzyme Techniques, Neurons analysis, Picrates, Time Factors, Tissue Distribution, Peptides analysis, Peripheral Nerves analysis
- Abstract
A method is described for the immunohistochemical localization of peptides in whole-mount preparations. Tissue is fixed as laminae with a picric acid/formaldehyde mixture and then dehydrated, cleared and rehydrated before exposure to antibodies. This procedure ensures adequate penetration of the antibody molecules without the need to freeze and thaw the tissue or to use detergents, preserves antigenicity and lowers non-specific background staining. The laminae are incubated with the primary antisera for 16 h at room temperature and, after washing, with a second, fluorescent tagged, antiserum. This can be followed by a peroxidase-anti-peroxidase localization of the second antiserum, which acts as a bridge. The method gives a precise and reproducible localization of immunoreactive peptides, with good penetration and low background even in thick preparation. Large areas can be scanned and neuroeffector relationships studied more easily than in sections.
- Published
- 1980
- Full Text
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320. [Bombesin and monoamine-containing cells of the rat stomach].
- Author
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Solov'eva IA, Buffa R, Marshavina LS, Kocherginskiĭ EB, and Kazakov VK
- Subjects
- Animals, Fluorescent Antibody Technique, Gastric Acid metabolism, Gastrin-Releasing Peptide, Histamine metabolism, Immunoenzyme Techniques, Peptides pharmacology, Rats, Serotonin metabolism, Biogenic Amines metabolism, Bombesin pharmacology, Chromaffin System metabolism, Enterochromaffin Cells metabolism, Gastric Mucosa metabolism
- Abstract
Revealing of enterochromaffine and enterochromaffine-like cells of the rat stomach was undertaken under the effect of bombesin and alpha- and beta-adrenoblocking agents. Fibers containing gastrin-releasing-peptide were immunohistochemically revealed in neural plexus of the rat digestive tract. Spontaneous rhythmic release of the stomach monoamine cells' secret was shown. The effect of bombesin on the enterochromaffine-like cells of the rat stomach fundus containing histamine, was shown to be actualized through beta-adrenoreceptors. Serotonin excretion from the enterochromaffine cells of the stomach antrum depends neither on bombesin nor on the adrenergic effects.
- Published
- 1986
321. Localization of bombesin and GRP (gastrin releasing peptide) sequences in gut nerves or endocrine cells.
- Author
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Buffa R, Solovieva I, Fiocca R, Giorgino S, Rindi G, Solcia E, Mochizuchi T, Yanaihara C, and Yanaihara N
- Subjects
- Animals, Chickens, Gastrin-Releasing Peptide, Guinea Pigs, Histocytochemistry, Immunochemistry, Rats, Species Specificity, Swine immunology, Bombesin analysis, Digestive System innervation, Endocrine Glands analysis, Peptides analysis
- Abstract
Five anti-gastrin releasing peptide (GRP) sera have been characterized against GRP, bombesin and related polypeptides spotted on cellulose acetate discs. Antibodies reacting with the C-terminal G-14 sequence of bombesin and the 19-27 sequence of GRP, were detected in all sera. Antibodies directed exclusively against the bombesin unrelated 1-17 sequence of GRP were found only in one serum (R-6902). With parallel immunohistochemical tests only the C-terminal immunoreactivity was detected in endocrine-paracrine cells of the chicken proventriculus, while both immunoreactivities were present in nerve fibres and a few nerve cell bodies of the mammalian gut. The distribution of GRP- and bombesin-like immunoreactive nerves in the gastric mucosa of both pyloric and oxyntic type the submucosal and myenteric plexus along the whole gastrointestinal wall and at sphincter regions is detailed.
- Published
- 1982
- Full Text
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322. Immunohistochemical demonstration of a dermorphin-like peptide in the rat brain.
- Author
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Buffa R, Solcia E, Magnoni E, Rindi G, Negri L, and Melchiorri P
- Subjects
- Animals, Gonadotropin-Releasing Hormone metabolism, Histocytochemistry, Male, Oligopeptides immunology, Oligopeptides physiology, Opioid Peptides, Rabbits, Rats, Brain Chemistry, Oligopeptides analysis
- Published
- 1982
- Full Text
- View/download PDF
323. [Effect of exogenous peptides on the endocrine cells of the antral portion of the rat stomach].
- Author
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Solov'eva IA, Kotel'nikova VI, Buffa R, and Giorgino S
- Subjects
- Animals, Enkephalin, Leucine, Enkephalins pharmacology, Gastrins immunology, Gastrins pharmacology, Immune Sera pharmacology, Rats, Secretin pharmacology, Chromaffin System drug effects, Enterochromaffin Cells drug effects, Peptides pharmacology, Pyloric Antrum drug effects
- Published
- 1982
324. Chromogranin A, B and C immunoreactivities of mammalian endocrine cells. Distribution, distinction from costored hormones/prohormones and relationship with the argyrophil component of secretory granules.
- Author
-
Rindi G, Buffa R, Sessa F, Tortora O, and Solcia E
- Subjects
- Adrenal Medulla cytology, Animals, Chromogranin A, Humans, Immune Sera, Intestines cytology, Islets of Langerhans cytology, Organ Specificity, Species Specificity, Thyroid Gland cytology, Chromogranins analysis, Cytoplasmic Granules ultrastructure, Endocrine Glands cytology, Nerve Tissue Proteins analysis, Proteins
- Abstract
Antibodies specific for chromogranin A, B or C have been used to detect immunohistochemically these three anionic proteins. Pancreatic A, B and PP cells, gut argentaffin EC, argyrophil ECL and gastrin G cells, thyroid C cells, parathyroid cells, adrenal medullary cells, pituitary TSH, FSH and LH cells as well as some axons of visceral nerves have been found to react with chromogranin A antibodies. Pancreatic A, gut EC and G, adrenal medullary and pituitary cells as well as some gut nerve fibers showed chromogranin B immunoreactivity. Chromogranin C immunoreactivity has been detected in pancreatic A, pyloric D1, intestinal L, thyroid C, adrenal medullary and pituitary cells, as well as in some gut neurons and nerve fibers. No crossreactivity has been found in immunohistochemical tests between chromogranins A, B or C and costored monoamines or peptide hormones/prohormones, from which chromogranins can be separated by selective extraction during fixation. On both morphological and chemical grounds a relationship seems to exist between chromogranin A and Grimelius' argyrophilia. Sialooligosaccharide chains of chromogranin A and, possibly, chromogranins' phosphoserine/phosphothreonine groups, seem to interact with guanidyl, amino, and/or imidazole groups of non-chromogranin components to form silver complexing sites accounting for granules' argyrophilia, which can be removed or blocked without affecting chromogranin immunoreactivities. The abundant anionic groups of the three proteins should contribute substantially to granules' basophilia, the partly "masked" pattern of which supports the existence of a close interaction of such groups with other components of secretory granules, including monoamines and peptide hormones or prohormones. Chromogranins could play a rôle in hormone postranslational biosynthesis and intragranular packaging.
- Published
- 1986
- Full Text
- View/download PDF
325. Immunohistochemical identification of the cholecystokinin cell in the intestinal mucosa.
- Author
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Buffa R, Solcia E, and Go VL
- Subjects
- Animals, Cross Reactions, Dogs, Duodenum analysis, Epithelial Cells, Epithelium analysis, Fluorescent Antibody Technique, Immune Sera isolation & purification, Intestinal Mucosa analysis, Iodine Radioisotopes, Jejunum analysis, Methods, Pylorus analysis, Pylorus cytology, Rabbits, Duodenum cytology, Intestinal Mucosa cytology, Jejunum cytology
- Abstract
In indirect immunofluorescence tests, antibodies against pure porcine cholecystokinin (CCK) have detected specific CCK cells in the duodenal and jejunal mucosa of the dog and man. The CCK cells were scattered in the epithelium of the crypts, although some were in the villi. No CCK cells were found in the stomach, pancreas, terminal ileum, or colon. Some pyloric G cells also showed some reactivity with CCK antiserum, but absorption of CCK antiserum with gastrin C terminal pentapeptide prevented the staining of pyloric cells and provided specific staining of intestinal CCK cells. Anti-human gastrin I serum stained some intestinal cells too. Most of such cells did not react when gastrin antiserum was absorbed with pure CCK (a treatment that did not prevent the staining of pyloric gastrin cells); they were interpreted as cross-reacting CCK cells rather than as intestinal gastrin cells.
- Published
- 1976
326. Endocrine cells producing regulatory peptides.
- Author
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Solcia E, Usellini L, Buffa R, Rindi G, Villani L, Zampatti C, and Silini E
- Subjects
- Animals, Endocrine Glands metabolism, Histocytochemistry methods, Humans, Immunoassay methods, Neuropeptides analysis, Endocrine Glands cytology, Neuropeptides biosynthesis
- Abstract
Recent data on the immunolocalization of regulatory peptides and related propeptide sequences in endocrine cells and tumors of the gastrointestinal tract, pancreas, lung, thyroid, pituitary (ACTH and opioids), adrenals and paraganglia have been revised and discussed. Gastrin, xenopsin, cholecystokinin (CCK), somatostatin, motilin, secretin, GIP (gastric inhibitory polypeptide), neurotensin, glicentin/glucagon-37 and PYY (peptide tyrosine tyrosine) are the main products of gastrointestinal endocrine cells; glucagon, CRF (corticotropin releasing factor), somatostatin, PP (pancreatic polypeptide) and GRF (growth hormone releasing factor), in addition to insulin, are produced in pancreatic islet cells; bombesin-related peptides are the main markers of pulmonary endocrine cells; calcitonin and CGRP (calcitonin gene-related peptide) occur in thyroid and extrathyroid C cells; ACTH and endorphins in anterior and intermediate lobe pituitary cells, alpha-MSH and CLIP (corticotropin-like intermediate lobe peptide) in intermediate lobe cells; met- and leu-enkephalins and related peptides in adrenal medullary and paraganglionic cells as well as in some gut (enterochromaffin) cells; NPY (neuropeptide Y) in adrenaline-type adrenal medullary cells, etc.. Both tissue-appropriate and tissue-inappropriate regulatory peptides are produced by endocrine tumours, with inappropriate peptides mostly produced by malignant tumours.
- Published
- 1987
- Full Text
- View/download PDF
327. Immunohistochemistry of brain 5-hydroxytryptamine.
- Author
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Buffa R, Crivelli O, Lavarini C, Sessa F, Verme G, and Solcia E
- Subjects
- Animals, Corpus Callosum analysis, Corpus Striatum analysis, Cross Reactions, Fluorescent Antibody Technique, Male, Neurons analysis, Occipital Lobe analysis, Radioimmunoassay, Rats, Telencephalon analysis, Brain Chemistry, Serotonin analysis
- Abstract
An immunohistochemical assay for 5-hydroxytryptamine (5HT) has been developed, validated by parallel radioimmunoassay and a series of tests with monoamines or related molecules, and applied to the detection of 5HT in rat brain sections. The procedure seems to be more sensitive and specific than the classical Falck-Hillarp technique. Among amines and related compounds tested, only 5-methoxytryptamine has been found to cross-react. 5HT-immunoreactive neurons and/or fibres have been observed in the spinal cord, brain stem, hypothalamic nuclei, epiphysis and subcommissural organ, thalamus, striatum, corpus callosum, amygdala, hippocampus, olfactory tubercle, and cerebral cortex.
- Published
- 1980
- Full Text
- View/download PDF
328. Bombesin-related peptides in the diffuse neuroendocrine system.
- Author
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Solcia E, Buffa R, Gini A, Capella C, Rindi G, and Polak JM
- Subjects
- Animals, Bombesin analysis, Gastrin-Releasing Peptide, Humans, Neurosecretory Systems cytology, Peptides analysis, Bombesin physiology, Neurosecretory Systems physiology, Peptides physiology
- Published
- 1988
- Full Text
- View/download PDF
329. Cytochemical and ultrastructural differentiation of enteroglucagon and pancreatic-type glucagon cells of the gastrointestinal tract.
- Author
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Grimelius L, Capella C, Buffa R, Polak JM, Pearse AG, and Solcia E
- Subjects
- Animals, Cats, Colon ultrastructure, Digestive System analysis, Dogs, Duodenum ultrastructure, Humans, Ileum ultrastructure, Intestinal Mucosa analysis, Intestinal Mucosa ultrastructure, Jejunum ultrastructure, Pancreas ultrastructure, Rats, Stomach ultrastructure, Digestive System ultrastructure, Glucagon analysis
- Abstract
Coordinated studies have been carried out on the glucagon immunoreactive cells of the mammalian gastrointestinal tract (man, dog, rat), using electron microscopy, silver staining and immunocytochemistry. Parallel ultrastructural and immunocytochemical studies have been made with the semithin-thin serial section technique. The results indicate that while the glucagon cells of the oxyntic portion of the stomach are virtually indistinguishable from those of the pancreatic islets (A cells) those of the intestine (EG cells) are completely different. Proper identification of glucagon immunoreactive cells requires the application of morphological and silver staining techniques, at the ultrastructural level.
- Published
- 1976
- Full Text
- View/download PDF
330. Identification, ultrastructure and classification of gut endocrine cells and related growths.
- Author
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Solcia E, Capella C, Buffa R, Fiocca R, Frigerio B, and Usellini L
- Subjects
- Animals, Carcinoid Tumor pathology, Cytoplasmic Granules ultrastructure, Digestive System innervation, Endocrine System Diseases pathology, Gastric Mucosa pathology, Humans, Intestinal Mucosa pathology, Microscopy, Electron, Endocrine Glands cytology, Gastric Mucosa cytology, Gastrointestinal Hormones metabolism, Gastrointestinal Neoplasms pathology, Intestinal Mucosa cytology
- Published
- 1980
331. Far-wing study of laser-induced collisional energy transfer.
- Author
-
Matera M, Mazzoni M, Buffa R, Cavalieri S, and Arimondo E
- Published
- 1987
- Full Text
- View/download PDF
332. Ultrastructural and histological study of 11 bronchial carcinoids. Evidence for different types.
- Author
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Capella C, Gabrielli M, Polak JM, Buffa R, Solcia E, and Bordi C
- Subjects
- Adult, Aged, Bronchial Neoplasms metabolism, Carcinoma, Adenoid Cystic metabolism, Female, Humans, Male, Microscopy, Electron, Middle Aged, Serotonin metabolism, Bronchial Neoplasms ultrastructure, Carcinoma, Adenoid Cystic ultrastructure
- Abstract
Seven of eleven bronchial carcinoids investigated showed cells with small granules resembling P cells which have already been described in human fetal and adult lung; two of these P cell tumours showed distinctive paraganglioid features. One tumour showed peculiar ultrastructural findings resembling closely those previously reported by Black (1969) in a so called "pulmonary oncocytoma". Three remaining cases showed large secretory granules resembling those of type 3 cells already described by Hage (1973b) in bronchial carcinoids; one of these tumours produced large amounts of 5-hydroxytryptamine (5HT). It is concluded that, on cytological grounds, at least two types of tumours can be distinguished among bronchial carcinoids, i.e. P cell and type 3 cell tumours. Moreover, two varieties of P cell carcinoids have been recognized, showing either the less frequent and more distinctive paraganglioid structure or the more common trabecular structure.
- Published
- 1979
- Full Text
- View/download PDF
333. Vasoactive intestinal peptide (VIP) cells in the pancrease and gastro-intestinal mucosa. An immunohistochemical and ultrastructural study.
- Author
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Buffa R, Capella C, Solcia E, Frigerio B, and Said SI
- Subjects
- Animals, Cytoplasmic Granules ultrastructure, Dogs, Fluorescent Antibody Technique, Gastric Mucosa ultrastructure, Guinea Pigs, Humans, Immunoenzyme Techniques, Intestinal Mucosa ultrastructure, Islets of Langerhans analysis, Islets of Langerhans ultrastructure, Microscopy, Electron, Pancreas ultrastructure, Gastric Mucosa analysis, Gastrointestinal Hormones analysis, Intestinal Mucosa analysis, Pancreas analysis, Vasoactive Intestinal Peptide analysis
- Abstract
Using antibodies against pure porcine VIP in immunoperoxidase and immunofluorescence tests, VIP-immunoreactive cells have been detected in the pancreas-especially in the islets-and gastrointestinal mucosa of the dog, guinea-pig and man. VIP immunoreactive cells were widely distributed in these tissues, never being numerous at any site. Some parallelism has been noted between such cells and ultrastructurally identified D1 cells fo the pancreas and gastrointestinal mucosa. The presence of VIP cells in normal pancreas may help explain the occurrence of pancreatic endocrine tumors producing VIP.
- Published
- 1977
- Full Text
- View/download PDF
334. Alpha and beta subunits of glycoprotein hormones in argyrophil pituitary tumors with small granule cells.
- Author
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Capella C, Buffa R, Usellini L, Frigerio B, Jehenson P, Sessa F, and Solcia E
- Subjects
- Adenoma analysis, Adolescent, Adrenocorticotropic Hormone metabolism, Adult, Aged, Child, Cytoplasmic Granules ultrastructure, Female, Growth Hormone metabolism, Histocytochemistry, Humans, Male, Middle Aged, Pituitary Neoplasms analysis, Pituitary Neoplasms pathology, Prolactin metabolism, Adenoma ultrastructure, Chorionic Gonadotropin analysis, Follicle Stimulating Hormone analysis, Luteinizing Hormone analysis, Pituitary Neoplasms ultrastructure, Thyrotropin analysis
- Abstract
A group of 33 functionless pituitary tumors with small argyrophil groups (SAG) were collected from a series of 200 pituitary adenomas (16.5% of all adenomas). Histologically, the tumors showed an unusually high frequency of trabecular patterns, perivascular pseudo-rosettes, and oncocytoid transformation. Immunoreactivity for glycoprotein hormone alpha-chain was found in more or less numerous cells of 20 cases (64.5% of SAG tumors). Thirteen of these cases also showed specific beta-chain immunoreactivity, especially for follicle-stimulating hormone (FSH) beta-chain, which was present in 11 tumors. Various admixtures of immature, oncocytic, sparsely granulated, and densely granulated cells were observed ultrastructurally, with prevalence of the latter cell variants in tumors showing immunoreactive cells and prevalence of the former cell variants in tumors lacking immunoreactive cells. It is suggested that some relationship may exist between SAG cell (glycoprotein hormone precursor cells?) tumors--or at least part of them--and glycoprotein hormone cell lines. Anyway, whatever their origin and interpretation, SAG cell tumors seem to represent a distinct clinicopathologic entity.
- Published
- 1983
- Full Text
- View/download PDF
335. Endocrine cells of the gastrointestinal tract: general aspects, ultrastructure and tumor pathology.
- Author
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Solcia E, Capella C, Buffa R, Usellini L, Fontana P, and Frigerio B
- Subjects
- Carcinoid Tumor classification, Carcinoid Tumor metabolism, Gastric Mucosa pathology, Gastrointestinal Neoplasms classification, Gastrointestinal Neoplasms metabolism, Humans, Intestinal Mucosa pathology, Paraneoplastic Endocrine Syndromes pathology, Peptides metabolism, Carcinoid Tumor ultrastructure, Gastric Mucosa cytology, Gastrointestinal Hormones metabolism, Gastrointestinal Neoplasms ultrastructure, Intestinal Mucosa cytology
- Published
- 1978
- Full Text
- View/download PDF
336. The diffuse endocrine-paracrine system of the gut in health and disease: ultrastructural features.
- Author
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Solcia E, Capella C, Buffa R, Usellini L, Fiocca R, Frigerio B, Tenti P, and Sessa F
- Subjects
- Animals, Autonomic Nervous System anatomy & histology, Cytoplasmic Granules ultrastructure, Digestive System innervation, Gastrointestinal Neoplasms ultrastructure, Humans, Microscopy, Electron, Pancreatic Neoplasms ultrastructure, Gastric Mucosa ultrastructure, Gastrointestinal Hormones metabolism, Intestinal Mucosa ultrastructure, Neurotransmitter Agents metabolism
- Abstract
At least 16 types of endocrine-paracrine cells have been identified ultrastructurally in the gastrointestinal mucosa. The production of hormones and local messengers such as 5-hydroxytryptamine, gastrin, cholecystokinin, somatostatin, secretin, gastric inhibitory peptide (GIP), enteroglucagon (glicentin, GLI), motilin, neurotensin, substance P and the enkephalins, by these cells, has been established. Progress has also been made in cytological studies of gut and pancreatic endocrine tumours. Argentaffin EC cell carcinoids, gastrinomas (of several ultrastructurally different varieties of gastrin cells), L-cell tumours and D-cell tumours are among those cytologically and functionally defined in the gut. Functionally undefined tumours include the so-called non-argentaffin carcinoids arising in various parts of the gut, some of which have been characterised cytologically as gastric ECL cell tumours and gastroduodenal P-D1-cell tumours. Gastrinomas, vipomas and rare argentaffin carcinoids are among gut-related pancreatic endocrine tumours. Non-functional paragangliomas, usually with some neuromatous component, occur in the duodenal wall. Extrapancreatic vipomas display ultrastructural features of ganglioneuroblastomas with peptidergic granules.
- Published
- 1981
337. [Tumors of the adenohypophysis: morphological diagnosis and prognosis].
- Author
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Solcia E, Capella C, Buffa R, Frigerio B, Fontana P, and Usellini L
- Subjects
- Humans, Pituitary Gland, Anterior pathology, Pituitary Gland, Anterior ultrastructure, Pituitary Neoplasms classification, Pituitary Neoplasms pathology, Pituitary Neoplasms ultrastructure, Prognosis, Pituitary Neoplasms diagnosis
- Published
- 1977
338. [Histochemistry and ultrastructure of endocrine cells of the digestive system].
- Author
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Solcia E, Capella C, Buffa R, Bettini R, and Vassallo G
- Subjects
- Animals, Chromaffin System ultrastructure, Dogs, Enterochromaffin Cells metabolism, Gastric Mucosa ultrastructure, Gastrins metabolism, Gastrointestinal Hormones metabolism, Glucagon metabolism, Histocytochemistry, Humans, Microscopy, Electron, Rabbits, Digestive System ultrastructure, Endocrine Glands ultrastructure
- Published
- 1974
339. Endocrine cells of the gastrointestinal tract and related tumors.
- Author
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Solcia E, Capella C, Buffa R, Usellini L, Frigerio B, and Fontana P
- Subjects
- Animals, Cytoplasmic Granules ultrastructure, Endocrine Glands physiology, Enterochromaffin Cells cytology, Gastrointestinal Hormones metabolism, Gastrointestinal Motility, Humans, Hyperplasia, Pancreatic Neoplasms pathology, Carcinoid Tumor pathology, Digestive System cytology, Endocrine Glands cytology, Endocrine System Diseases pathology, Gastrointestinal Neoplasms pathology
- Published
- 1979
340. Histochemical and ultrastructural identification of neurotensin cells in the dog ileum.
- Author
-
Frigerio B, Ravazola M, Ito S, Buffa R, Capella C, Solcia E, and Orci L
- Subjects
- Animals, Antigens, Dogs, Enterochromaffin Cells cytology, Glucagon immunology, Immunoenzyme Techniques, Intestine, Small cytology, Pancreatic Hormones immunology, Peptides immunology, Staining and Labeling, Ileum cytology, Neurotensin immunology
- Abstract
In the dog ileum, neurotensin cells stained with immunofluorescence or immunoperoxidase proved distinct from argentaffin (EC) cells, glucagon immunoreactive (GLI) cells and pancreatic peptide immunoreactive (PP) cells. Neurotensin cells showed various degrees of reactivity with Grimelius' silver. With electron microscopy, besides EC cells, large granule cells with a thin peripheral rim of Grimelius-reactivity (L cells) and large granule cells with variable Grimelius-reactivity of the core (N cells) were found. On distributive grounds, L cells were identified with GLI cells and N cells were interpreted as neurotensin cells.
- Published
- 1977
- Full Text
- View/download PDF
341. Endocrine cells of the gastric mucosa.
- Author
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Solcia E, Capella C, Vassallo G, and Buffa R
- Subjects
- Animals, Chromaffin System metabolism, Cytoplasmic Granules ultrastructure, Duodenum cytology, Enterochromaffin Cells cytology, Enterochromaffin Cells ultrastructure, Gastric Mucosa metabolism, Gastric Mucosa ultrastructure, Gastrins metabolism, Glucagon metabolism, Histamine Release, Histocytochemistry, Humans, Intestinal Mucosa cytology, Pancreas cytology, Pylorus cytology, Serotonin analysis, Species Specificity, Staining and Labeling, Chromaffin System cytology, Gastric Mucosa cytology
- Published
- 1975
- Full Text
- View/download PDF
342. Calbindin 28 kDa in endocrine cells of known or putative calcium-regulating function. Thyro-parathyroid C cells, gastric ECL cells, intestinal secretin and enteroglucagon cells, pancreatic glucagon, insulin and PP cells, adrenal medullary NA cells and some pituitary (TSH?) cells.
- Author
-
Buffa R, Mare P, Salvadore M, Solcia E, Furness JB, and Lawson DE
- Subjects
- Animals, Calbindins, Calcium metabolism, Cats, Chickens, Dogs, Ducks, Endocrine Glands cytology, Gastric Mucosa metabolism, Guinea Pigs, Humans, Immunohistochemistry, Intestinal Mucosa metabolism, Quail, Rats, Endocrine Glands metabolism, S100 Calcium Binding Protein G metabolism
- Abstract
The distribution of calbindin in some endocrine glands (thyroid, parathyroid, ultimobranchial body, pituitary and adrenals) and in the diffuse endocrine cells of the gut and pancreas has been investigated immunohistochemically using an antiserum raised against the 28 kDa calbindin from chicken duodenum. The identity of calbindin-immunoreactive cells in a number of avian and mammalian species was ascertained by comparison with hormone-reactive cells in consecutive sections or by double immunostaining of the same section with both calbindin and hormone antibodies. Calcitonin-producing C cells of the mammalian and avian thyroid, parathyroid or ultimobranchial body, PP, glucagon and insulin cells of the mammalian and avian pancreas, enteroglucagon cells of the avian intestine, secretin cells of the mammalian duodenum, histamine-producing ECL cells of the mammalian stomach, as well as noradrenaline-producing cells of the adrenal medulla and some (TSH?) cells of the adenohypophysis were among the calbindin-immunoreactive cells. Although some species variability has been observed in the intensity and distribution of the immunoreactivity, especially in the pancreas and the gut, a role for calbindin in the mechanisms of calcium-mediated endocrine cell stimulation or of intracellular and extracellular calcium homeostasis is suggested.
- Published
- 1989
- Full Text
- View/download PDF
343. Complement-mediated unspecific binding of immunoglobulins to some endocrine cells.
- Author
-
Buffa R, Crivelli O, Fiocca R, Fontana P, and Solcia E
- Subjects
- Animals, Cricetinae, Guinea Pigs, Humans, Immunologic Techniques, Protein Binding, Rabbits, Swine, Complement System Proteins metabolism, Gastric Mucosa metabolism, Immunoglobulins metabolism, Islets of Langerhans metabolism
- Abstract
Unspecific binding of immunoglobulins to gastrin G cells, glucagon A cells and somatostatin D cells of the gastric mucosa or pancreas, as well as to the calcitonin-somatostatin cells of rabbit thyroid has been found to occur through a non antigen-antibody mechanism mediated at least in part by the C1q fraction of complement. The phenomenon represents a major drawback in hormone immunohistochemistry, which can be prevented by incubating the specific anti-hormone sera with anti-C1q antibodies or with complement-fixing immunocomplexes.
- Published
- 1979
- Full Text
- View/download PDF
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