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该文探讨了该校护理伦理学线下一流本科课程的建设情况。课程从教学理念、教学团队、教学 目标、教学设计、课程内容整合、课程思政及课程建设的组织与实施、课程建设成效评定等方面全方位进行改 革,提升了课程质量,丰富了教学内容,德育渗透作用较强,有助于提高学生的职业综合素养,总体效果满意。 课程组自我评价认为案例讨论和情景表演活动这些多元教学方法的组织有待进一步加强,需持续建设并不断 完善 [ABSTRACT FROM AUTHOR]

In this study, CMC-modified nano-Fe /Ni bimetallic materials were prepared to investigate the influence factors of CMC-nano-Ni-Fe bimetallic materials on the removal of p-nitrophenol from water. The results showed that the PNP removal efficiency of the material first increased and then decreased with the increase of nickel-iron ratio, and the optimal nickel-iron ratio was 0.025.With the increase of the initial concentration of PNP, the concentration first increased and then decreased, and the optimal concentration was 30 mg/L. The reaction is greatly affected by pH, and the removal rate of PNP is acidic ＞ neutral ＞ basic. When the pH is 5, the removal rate of PNP can reach 100% after 120 minutes of reaction. The infrared spectrum of CMC-Fe/Ni composite shows that the vibration absorption peak of CMC functional group decreases with the increase of Ni/Fe ratio, but the overall structure is relatively stable. [ABSTRACT FROM AUTHOR]

目的探讨结直肠癌根治术后癌胚抗原(CEA)的动态变化趋势与结直肠癌复发、预后的关系。方法165例结直肠癌根治术后患者,术后根据CEA变化趋势分为快速上升型45例(快速组)和缓慢上升型120例(缓慢组),分析CEA变化趋势与直肠癌临床病理特征及患者预后的关系。结果快速组淋巴结转移率明显高于、TNM分期为Ⅲ期者所占比例、术前CEA>5μg/L者所占比例明显高于缓慢组,P均<0.05。快速组复发转移率(86.7%)明显高于缓慢组(25%);多病灶转移率(76.9%)明显高于缓慢组(60.0%),P均<0.05。快速组Ⅲ期者复发转移率和病灶转移率更高,可再次行R0切除术的几率极小。快速组五年生存率低于、中位生存时间短于、中位无进展生存期短于缓慢组,P均<0.01。结论 CEA的动态变化可提示结直肠癌患者预后。 [ABSTRACT FROM AUTHOR]

The experiment was to evaluate the antioxidant capacity of total flavonoids of stem bark of Acanthopanax senticosus. The in vitro antioxidant assay and the establishment of a chicken embryonic fibroblast (CEF) oxidative damage model were used to evaluate the antioxidant capacity of total flavonoids from stem bark of Acanthopanax senticosus. The results showed that total flavonoids of Acanthopanax stoloniferum had strong ability to scavenge oxidative free radicals in vitro, and had protective effect on oxidatively damaged CEF cells, regulating the changes of T-AOC, SOD, GSH-Px activity and MDA content caused by oxidative damage. The study indicates that the total flavonoids of stem bark of Acanthopanax senticosus has strong antioxidant activity. [ABSTRACT FROM AUTHOR]

Feather is a sort of commonly used noninvasive material that can provide DNA and hormones for the research， monitoring and management of birds. However， feather vane is a fully keratinized tissue with very low content of DNA and hormones that often challenges the extraction. In the study， we developed a novel method to co-extract DNA and corticoste⁃ roid from feather vane， named DH-CoEx. The extraction efficiency and usability of DH-CoEx were evaluated by using feath⁃ ers from large feathers represented by the red crowned crane (Grus japonensis) and green peafowl (Pavo muticus) ， and small feathers represented by the chestnut bunting (Emberiza rutila) and little bunting (E. pusilla). The results showed that DH-CoEx could co-extracted total DNA and corticosteroid (cortisol) from feather vanes. The copy number of mitochondrial DNA (mtDNA) extracted from 5 mg feather reached 104 ， that could support amplification of 1，350 bp fragments. The nuclear DNA (nuDNA) could support genotyping of microsatellites with a success rate of 98. 11% - 99. 60% success rate for alleles less than 200 bp. The yield of cortisol co-extracted reach the level of normal single extraction approaches and supportive for physiological analysis. These results suggest that DH-CoEx is a simple and effective method to co-extract DNA and corticosteroids from a fully keratinized feather to support analysis of genetic markers and stress hormone in a broad range of application. [ABSTRACT FROM AUTHOR]

In this study, pesticide registration on kiwifruit was collated by querying the China Pesticide Information Network, and the maximum residue limit standard of pesticides for kiwifruit was collated according to China’s mandatory national standards GB 2763-2021 and GB 2763. 1-2022. The differences between China and CAC, the European Union, the United States, New Zealand, Japan and South Korea in the current kiwifruit limit standards were systematically analyzed, and suggestions were put forward on the revision of pesticide residue limit standards, safe drug use, quality control and reducing the risk of technical barriers, so as to provide reference for the high-quality development of kiwifruit industry in China. [ABSTRACT FROM AUTHOR]

Electrocatalytic water splitting exhibits outstanding advantages for solving the critical problems of clean energy shortage and environmental pollution. Electric driven water splitting is a promising strategy for producing clean and renewable hydrogen. Metal-organic frameworks (MOFs) and their derivatives are considered as ideal porous materials for electrocatalytic H2 production, resulting from their tailorable structure, ultra-large surface area, and design flexibility. This review starts with the reaction principle of electrocatalytic water splitting, relevant factors that determine the electrocatalytic activity, and then summarizes and exemplifies the recent progress in the development of MOFs based- and derived-electrocatalysts for water splitting. Finally, we highlighted the many challenges exist in the water splitting field, and proposed some perspectives of MOFs based- and derived-electrocatalysts for H2 production from water splitting. [ABSTRACT FROM AUTHOR]

AIM: To investigate the anti-fibrotic effect of Panax notoginseng saponins (PNS) in arecoline (ANE)-induced oral submucous fibrosis, and to analyze the effect of PNS on nuclear factor E2-related factor 2(Nrf2)/glutamate-cysteine ligase catalytic subunit (GCLC) signaling pathway. METHODS: CCK-8 assay was used to evaluate the effects of different concentrations of PNS and arecoline on the survival rate of human immortalized keratinocyte cell line HaCaT. The results of CCK-8 were used to select 75 mg/L arecoline, and 25，50 and 100 mg/L PNS as subsequent experimental concentrations. The cells were set as blank control group, model group, and low, medium and high doses (25，50 and 100 mg/L) of PNS groups. The protein and mRNA expressions of collagen type I (COL-I), E-cadherin, Nrf2, GCLC and glutathione reductase (GR) in each group were detected by Western blot and RT-qPCR. Immunofluorescence method was used to detect the entry of Nrf2 into the nucleus. Biochemical kits were used to detect the content of glutathione (GSH), nicotinamide adenine dinucleotide phosphate (NADPH) and malondialdehyde (MDA), and superoxide dismutase (SOD) activity in each group of cells. DCFH-DA fluorescent probe was used to detect the content of intracellular reactive oxygen species (ROS). RESULTS: Compared with the blank control group, the protein and mRNA expression of COL-I in the model group was up-regulated, and the protein and mRNA levels of E-cadherin, Nrf2, GCLC, nuclear Nrf2 and GR were down-regulated. The content of NADPH, MDA and ROS in the cells increased, and the content of GSH and the activity of SOD was significantly reduced. Compared with the model group, the protein and mRNA expression of COL-I was down-regulated, and the protein and mRNA expression of E-cadherin, Nrf2, GCLC, nuclear Nrf2 and GR were up-regulated in PNS 50 and 100 mg/L groups. Compared with the model group，the content of NADPH, MDA and ROS in cells decreased, and the content of GSH and the activity of SOD was significantly enhanced (P<0. 05 or P<0. 01). CONCLUSION:Panax notoginseng saponins have anti-fibrosis effects in HaCaT cells, and their mechanism may be related to the activation of Nrf2/GCLC signaling pathway, thereby resisting oxidative stress and improving oral submucosal fibrosis. [ABSTRACT FROM AUTHOR]

Objective To evaluate the service effect of health education institutions in China, identify the existing problems, and put fonvard feasible solutions. Methods The Technique for Order Preference by Similarity to an Ideal Solution (TOPSIS) and Rank-sum ratio (RSR) were used to evaluate the service effect of health education institutions in 31 provinces (autonomous regions and municipalities directly under the central government). Results In total 14 indicators for evaluating the sendee effectiveness of health education institutions were selected in the study. Among them, the top two index with the largest weight were the number of people covered by short messages and the number of audio and video products, while the last two were the number of host websites and the number of programs by co-operating with TV stations. The evaluation of entropy weight TOPSIS method showed that the Ci value of each region was between 0.022 and 0.665. The evaluation results of RSR method showed that the number of areas where the service effect of health education institutions was poor, average, good, and excellent were 2, 13, 13, and 3, respectively. The general trend of the two results was basically consistent with that of the fuzzy joint model. Conclusion The fuzzy combination of entropy weight TOPSIS method and RSR method can better e-valuate the sendee effect of health education institutions in China. There is an imbalance between the content and the region in the service of health education institutions. We should increase the total investment in health education publicity and human resources, and put forward measures according to the differences of different regions, so as to improve the service effect of health education institutions and enhance residentsJ well-being and satisfaction. [ABSTRACT FROM AUTHOR]

[Objective]To explore the involvement of SWEET(sugars will eventually be exported transporters)in sugar metabolism and response to abiotic stress in Camellia oleifera.[Method]Bioinformatics methods were used to analyze the gene structure, protein motif, chromosome localization, collinearity, cis-acting elements of promoter region and upstream regulators in CoSWEETs family, and RT-qPCR was used to analyze the expression pattern of CoSWEETs in different periods, tissues and stress responses.[Result]Total 14 CoSWEET genes were identified in Camellia oleifera for the first time, they were localized on 10 chromosomes, and there were differences in intron-exon numbers between different members. With 1-2 MtN3 conserved domain, the 14 CoSWEETs were divided into four subgroups by phylogenetic tree, and there were similar gene structures and motif in the same subgroup. There were differences in number of introns and exons among different members. Based on the analysis of promoter cis-acting elements and upstream transcription factor predictions, multiple cis-elements related to development, plant hormones and environmental stresses were found in the promoter region, and their expressions were regulated by transcription factors, like ERF, DOF, BBR-BPC and MYB. RT-qPCR results showed that CoSWEETs highly expressed in the fruit and root, and the expression in the seeds was related to the developmental stages. Moreover, several genes responding to low-temperature, drought or salt stress, such as CoSWEET1, CoSWEET2 and CoSWEET17 were mined based the expression profile of CoSWEETs members under abiotic stresses of low temperature, drought and high salt.[Conclusion]The expression of CoSWEETs is regulated by various hormones and transcription factors, and plays a crucial role in the development of seeds and the response to stress in Camellia oleifera. [ABSTRACT FROM AUTHOR]

Objective To analyze the influencing factors of survival of patients with airway stenosis requiring clinical interventions after lung transplantation. Methods Clinical data of 66 patients with airway stenosis requiring clinical interventions after lung transplantation were retrospectively analyzed. Univariate and multivariate Cox’s regression models were adopted to analyze the influencing factors of survival of all patients with airway stenosis and those with early airway stenosis. Kaplan-Meier method was used to calculate the overall survival and delineate the survival curve. Results For 66 patients with airway stenosis, the median airway stenosis-free time was 72 (52,102) d, 27% (18/66) for central airway stenosis and 73% (48/66) for distal airway stenosis. Postoperative mechanical ventilation time [hazard ratio (HR) 1.037, 95% confidence interval (CI) 1.005-1.070, P=0.024] and type of surgery (HR 0.400, 95%CI 0.177-0.903, P=0.027) were correlated with the survival of patients with airway stenosis after lung transplantation. The longer the postoperative mechanical ventilation time, the higher the risk of mortality of the recipients. The overall survival of airway stenosis recipients undergoing bilateral lung transplantation was better than that of their counterparts after single lung transplantation. Subgroup analysis showed that grade 3 primary graft dysfunction (PGD) (HR 4.577, 95%CI 1.439-14.555, P=0.010) and immunosuppressive drugs (HR 0.079, 95%CI 0.022-0.287, P<0.001) were associated with the survival of patients with early airway stenosis after lung transplantation. The overall survival of patients with early airway stenosis after lung transplantation without grade 3 PGD was better compared with that of those with grade 3 PGD. The overall survival of patients with early airway stenosis after lung transplantation treated with tacrolimus was superior to that of their counterparts treated with cyclosporine. Conclusions Long postoperative mechanical ventilation time, single lung transplantation, grade 3 PGD and use of cyclosporine may affect the survival of patients with airway stenosis after lung transplantation. [ABSTRACT FROM AUTHOR]

In response to the weak sense of professional mission and values among students caused by the insufficient integration of ideological and political elements with professional knowledge in chemistry experiment teaching, the Analytical Chemistry Experiment course organically incorporates ideological and political elements into the experimental content. It formulates the “Trinity” educational goals of integrating knowledge, skills, and qualities, establishes a “six-focus” ideological and political case library, implements the “seven-integration” curriculum ideological and political teaching measures, innovates the “five-interaction” ideological and political education design, and creates challenging classrooms with depth and warmth. It engages in dialogues with students on knowledge, thinking, and emotions. [ABSTRACT FROM AUTHOR]

In order to efficiently and accurately predict traffic conflicts between motorized and non-motorized vehicles at unsignalized roundabouts, a combined prediction model (SVR-GA-BP) based on genetic algorithm optimized BP neural network (GA-BP) and support vector machine regression (SVR) was proposed. The high-precision mixed traffic flow trajectory data was collected using drone video at an unsignalized roundabout. The video recognition software Tracker was used to extract the trajectory data of motorized and non-motorized vehicles conflicts. The time to collision (TTC) parameter was chosen as the discriminant index to determine the severity of motorized and non-motorized vehicles conflicts. Based on partial correlation analysis, the traffic volume, average speed and percentage of heavy vehicles were determined as the significant influencing factors for conflicts. Five evaluation metrics, such as root mean square error (RMSE) and mean absolute error (MAE), were selected to analyze the accuracy of the predicted values of SVR model, BP neural network and SVR-GA-BP model. The results show that the accuracy of the combined model in minor conflict prediction is 97. 1%, which is 6. 9% and 2. 5% higher than that of SVR and BP model respectively. The accuracy of the combined model in serious conflicts prediction is 96. 1%, which is 7. 3% and 5. 1% higher than that of SVR and BP model respectively. It can be seen that the SVR-GA-BP combined model can effectively predict the motorized and non-motorized vehicles traffic conflict of unsignalized roundabout with the highest accuracy, which can provide reference for the safety evaluation of the same type of intersections. [ABSTRACT FROM AUTHOR]

A new type of high-efficiency paraffin remover YH-11 which can be continuously added on site was developed by using heptane, light oil and xylene as the best compound organic solvent in the mass ratio of 1:2:1, and LHG・034 paraffin remover, ethylene glycol monobutyl ether mutual solvent and JFC-2 penetrant in the mass ratio of 6 : 5 : 0. 6・ The wax dissolving rate of the wax remover and inhibitor is 0. 062 g/min, the wax control rate is 75.3%, and the viscosity reduction rate is 48. 0% ・ The comprehensive application effects of wax removal, wax control, viscosity reduction and yield increase are effectively realized. [ABSTRACT FROM AUTHOR]

The experiment was to study the effect of Echinops latifolius Tausch flavonoids on immune and antioxidant indexes of mice. A total of 80 Kunming mice were randomly divided into four groups, with 20 replicates in each group and one mouse in each replicate. The mice in low dose group, middle dose group, and high dose group were gavaged with 100, 200 and 400 mg/kg Echinops latifolius Tausch flavonoids, respectively, and the mice in the blank control group were treated with the same amount of distilled water. The experiment lasted for 30 d. The results showed that compared with control group, the thymus and spleen indexes of the mice in each dose group were increased (P>0.05), and the thymus index of high-dose group was significantly higher than that of control group (P<0.05). The serum levels of IgG and IgA in high-dose group were significantly higher than those in control group (P<0.05), and the serum level of IgM was slightly higher than that in control group (P>0.05). The serum IgA level in medium-dose group was significantly higher than that in control group (P<0.05). Compared with control group, the serum levels of IL-1β in high-dose group and medium-dose group were extremely decreased (P<0.01), and the serum levels of IL-6 and TNF-α in high-dose group were significantly decreased (P<0.05). The serum IL-6 of mice in medium dose group was significantly rdecreased (P<0.05). The activity of SOD in serum of mice in high, medium and low dose groups were significantly higher than those in control group (P<0.05), and GSH-Px activity was extremely higher than control group (P<0.01). The serum T-AOC of mice in high and low dose group was extremely higher than that in control group (P<0.01), and the serum MDA content of mice in high dose group was extremely lower than that in control group (P<0.01). The study indicates that the Echinops latifolius Tausch flavonoids can promote the development of immune organs in mice, enhance the antioxidant capacity of mice, and improve the immune function of mice, and adding 400 mg/kg flavonoid solution has the best effect. [ABSTRACT FROM AUTHOR]

Solar greenhouses have been the main type of facility for tomato production in China. Low light in the facility is one of the most important restricting factors on tomato production, even leading to the decline of fruit quality and flavor. Phase supplementation can be expected to select the growth stage with strong sensitivity to low light. The low light intervention can be one of the economically feasible ways, in order to reduce the high cost of artificial light supplementation during the whole growth stage of tomato. This study aims to investigate the low light sensitivity of tomatoes at different growth stages. Tomato "Harvest 74-560RZ F1" was selected in a fully artificial light-based plant factory, where 1 000 μmol/(m² ·s) light was the control during the whole growth stage. Four treatments of low light were carried out at a certain period of time during the flowering, expanding, color turning, and harvesting stages. The low light intensity was 160 μmol/(m2 ·s). The light intensity of other growth periods remained at 1 000 μmol/(m2 ·s) in the same treatment. The sensitivity of tomato quality formation to low light at different growth stages was clarified to analyze the accumulation of secondary metabolites that related to the tomato flavor and quality. Experimental conditions were all the same except for the light. The results showed as follows: 1) All lowlight treatments decreased the contents of polyphenols, total flavonoids and lycopene. Among them, the total flavonoids and polyphenols contents of tomato were the lowest under low light treatment at the flowering stage among the treatments, which were significantly reduced by 35% and 29%, respectively, compared with the control (P<0.05). The lycopene content was lower under low light treatments at flowering and color-turning stages, which was significantly reduced by 45% and 60%, respectively, compared with the control (P<0.05). Lycopene was significantly and positively correlated with the total flavonoids and polyphenols content in tomato fruits under all treatments. 2) All low-light treatments significantly reduced the total amount and total content of volatile secondary metabolites, such as aldehydes, ketones, esters, hydrocarbons, and alcohols in tomato fruits (P<0.05). The highest and lowest contents were Aldehydes and alcohols under all treatments, respectively. The low light treatment at the expanding stage had the lowest total number of volatile compounds (40 species, 9% less than the control), while the lowest total content of volatile compounds was 28% less than the control at the flowering stage. Among them, the contents of aldehydes, esters and other volatile compounds were the lowest at flowering treatment, which was significantly reduced by 31.7%, 64.1% and 56.8%, respectively, compared with the control. The content of alcohols in tomato fruits was lower than that of other treatments under low light treatments at flowering and expanding stages, which was significantly reduced by 25.3% and 28.2%, respectively, compared with the control (P<0.05). Therefore, the low light stress at the flowering stage presented the greatest adverse effect on the accumulation of secondary metabolites related to fruit quality and flavor in tomatoes. The low light-sensitive stage of tomato flowering is required to be supplemented with artificial light sources. The intensity and quality of supplemental light can also be further investigated at the flowering stage, according to the expected performance and cost of supplemental light. [ABSTRACT FROM AUTHOR]

AIM: To investigate whether Panax notoginseng saponins (PNS) attenuate arecoline (ANE)-induced human oral submucous fibrosis (OSF) by regulating ferroptosis, and to study its mechanism. METHODS: The OSF model was established by treating human immortalized keratinocyte cell line HaCaT with ANE. There were 6 groups created: blank group, model group(50 mg/L ANE), low-, medium- and high-dose (12. 5, 25 and 50 mg/L)PNS groups, and liproxstatin-1 (ferroptosis inhibitor) group. The morphological changes of the cells were observed under a light microscope. Cell viabilty was detected by CCK-8 assay. Collagen type I (Col-l), solute carrier family 7 member 11(SLC7A11) and glutathione peroxidase 4(GPX4)protein expression levels were detected by Western blot. The morphological changes of mitochondria were observed by transmission electron microscopy. The Fe2+ level was detected by FerroOrange fluorescent probe. The intracellular reactive oxygen species (ROS) content was detected by DCFH-DA fluorescent probe. The content of glutathione (GSH)was determined by colorimetry. RESULTS: Compared with blank group, the majority of the cells in model group displayed long spindle shape, fibrotic appearance, unstable adhesion, and obvious morphological changes. Under electron microscope, mitochondrial double membrane density increased, and cristae decreased significantly, presenting typical ferroptosis. The expression of Col-I increased, GPX4 and SLC7A11 protein expression decreased, Fe2+ and ROS levels increased, and GSH content decreased (all/><0. 05). Compared with model group, most of the cells in low-, medium- and high-dose PNS groups were round and epit he lo id, with decreased mitochondrial membrane density, increased ridge number and normal morphology, decreased Col-I expression and ROS level, and increased GSH content (all P<0. 05). Compared with model group, the protein expression levels of GPX4 and SLC7A11 in medium- and high-dose PNS groups were significantly increased, while the content of Fe2+ was significantly decreased (all P<0. 05). There were no significant differences in cell and mitochondrial morphology and indicator levels between liproxstatin-1 group and high-dose PNS group (all 05). CONCLUSION: Panax notoginseng saponins can alleviate OSF by preventing the ferroptosis of HaCaT cells induced by ANE. [ABSTRACT FROM AUTHOR]

AIM: To investigate the expression of syntaxin binding protein 4 (STXBP4) in oral squamous cell carcinoma (OSCC) and its effects on the malignant biological behavior of OSCC cells. METHODS: (1) The TIMER, GEPIA2, UALCAN, ENCORI, GEO and HPA databases were used to comprehensively study the expression of STXBP4 in head and neck squamous cell carcinoma (HNSCC) tissues at mRNA and protein levels. The mRNA and protein expres⁃ sion levels of STXBP4 in OSCC cell lines were detected by qPCR and Western blot, respectively. (2) The expression of STXBP4 in SCC-9 cells was down-regulated by small interfering RNA (siRNA), and knockdown (si-STXBP4-1, si-STXBP4-2 and si-STXBP4-3) groups and negative control (si-NC) group were established. Overexpression of SCAL-27 cells was achieved by lentivirus transfection, and overexpression (LV-STXBP4) group and negative control (LV-NC) group were established. The viability, colony formation and migration of OSCC cells were examined by CCK-8, plate colony formation, and Transwell assays, respectively. Western blot was used to detect the expression of epithelial-mesen⁃ chymal transition (EMT)-related proteins. (3) By establishing a subcutaneous xenograft tumor model in nude mice, we in⁃ vestigated the effect of STXBP4 on the proliferation of OSCC cells in vivo. RESULTS: (1) Analysis of the TIMER, GE⁃ PIA2, UALCAN, ENCORI and GEO databases revealed that HNSCC tissues had higher STXBP4 mRNA levels than nor⁃ mal tissues. Immunohistochemical staining from HPA database showed that STXBP4 protein in HNSCC tissues was higher than that in normal oral mucosa tissues. In contrast to normal oral mucosa cells, STXBP4 was highly expressed in 5 OSCC cell lines (P<0. 05). (2) The relative expression of STXBP4 in SCC-9 cells transfected with si-STXBP4-1, si-STXBP4-2 and si-STXBP4-3 was significantly lower than that in si-NC group (P<0. 01). Compared with si-NC group, knockdown of STXBP4 resulted in decreased proliferation and migration of SCC-9 cells (P<0. 05). The CAL-27 cells transfected with LV-STXBP4 had considerably higher STXBP4 level than the cells in LV-NC group (P<0. 01), and had markedly better proliferation and migration abilities than those in LV-NC group (P<0. 01). The protein expression of N-cadherin decreased and that of E-cadherin increased after STXBP4 expression was knocked down, while STXBP4 overexpression exerted the opposite effects (P<0. 05). (3)In vivo, overexpression of STXBP4 increased the volume and weight of xenograft tumors in nude mice (P<0. 05). CONCLUSION: High expression of STXBP4 promotes the proliferation and migration of OSCC cells, and its effect on the migration behavior of OSCC cells may be related to EMT. [ABSTRACT FROM AUTHOR]

Because of the problems of easy caking of fermented manure during long-term stacking and the difficulty of fertilizer crushing and striping, a solid organic fertilizer crushing and striping method combined with propeller blades and differential roller was proposed to solve the problems such as fertilizer caking and overhead fertilizer boxes, difficulty in strip application, and uneven fertilization amount during the operation of traditional chain-type fertilizer transport devices. Hertz-Mindlin with bonding and Hertz-Mindlin with JKR models were combined to establish the discrete element model of solid organic fertilizer composed of caked and bulk fertilizers. The process of fertilizer transport and fertilizer crushing of the paddle was studied with the quality change of fertilizer particles, the torque of propeller blades, the force of organic fertilizer particles, and the average movement speed as evaluation indicators. The stress distribution of the blade and the fertilizer box during transporting and crushing fertilizer was investigated by force analysis, the working process of the trenching fertilizer shovel and the compaction wheel was analyzed, and the influence of the change of the shovel tip angle and the compaction angle on the soil resistance during the trenching and compaction process was clarified. The discrete element simulation analysis results shown that the average torque of the crushing stage in fertilizer crushing was 52.05 and 58.75 N·m, the average force of particles was 343.25 and 374.38 N,wich were much higher than the average torque of transport fertilizer stage in fertilizer transportation(20.42 N·m and 224.22 N). The movement speed of organic fertilizer particles was stable at about 0.6m/s in the process of fertilizer transportation and fertilizer crushing, and the particle mass was stable without apparent fluctuation. The area with significant force position was mainly concentrated at the bottom and side wall of the blade and fertilizer box. Based on the above analysis results, a solid organic fertilizer crushing and striping machine was developed, and field experiments were conducted. The results showed that the average coefficient of variation of fertilization amount under 9 sets operation parameter combinations was 21.5%. The optimal working parameter combination of the strip applicator was the compaction inclination angle of 120 °, the forward velocity of 5 km/h, fertilization depth of 150 mm, and the obtained coefficient of variation of fertilization amount was 15.2%, and the fertilization process was stable and reliable. The research results could provide equipment support for protecting arable land and applying solid organic fertilizer crushing in the black soil area of Northeast China. [ABSTRACT FROM AUTHOR]

【Objective】The study aimed to explore the molecular characteristics and the expression regulation pattern of STR gene（CsSTR） in tea plant（Camellia sinensis）, so as to lay the foundation for further functional analysis of CsSTR gene and for mining the medicinal value of tea. 【Method】By employing the RACE technology, CsSTR1 gene was cloned. The physicochemical properties, protein structure, subcellular localization and phylogenetic relationship of CsSTR1 protein were studied by the methods of bioinformatics. Expression pattern of CsSTR1 gene was analyzed by qPCR. 【Result】The full length of CsSTR1 cDNA was 1382 bp, containing 92 bp 5′ UTR, 159 bp 3′ UTR, 1131 bp CDS. The CsSTR1 protein had 376 aa, with the molecular weight being 41.5 kD, the theoretical isoelectric point being 6.36, the instability index being 29.74, the aliphatic index being 88.94, and the grand average of hydropathicity being-0.077. Subcelluar analysis revealed that the CsSTR1 protein was localized in vacuole. The proportions of random coil, extended strand, α-helix and β-turn in the secondary structure of CsSTR1 protein were 42.82%, 29.79%, 16.76%, and 10.64%, respectively. The three-dimensional structure of CsSTR1 protein displayed as six-bladed β-propeller. The phylogenetic analysis revealed a close genetic relationship between CsSTR1 and rice OsSTRL2 protein. qPCR results showed that CsSTR1 gene was expressed in various organs of tea plant, with higher expression levels in buds, the first leaf, the second leaf and the third leaf, with lower expression levels in flowers and fruits. Up-regulation of CsSTR1 gene was seen under low temperature, PEG simulated drought stress, MeJA and SA treatments; Expression of CsSTR1 gene was down-regulated by ABA after 24-48 hours of the treatment. 【Conclusion】The results suggest that CsSTR1 has an important role in both the buds and leaves development of tea plant and defense response to adversity stress, MeJA, SA and ABA can regulate the CsSTR1 gene expression. [ABSTRACT FROM AUTHOR]

BACKGROUND: Radiotherapy is one of the main methods for the treatment of brain tumors, which significantly improves the clinical efficacy. However, its side effects on normal brain tissue seriously affect the quality of life of patients. Therefore, it is particularly important to find a way to effectively prevent the damage of normal brain tissue and to study its mechanism. OBJECTIVE: To explore the effect and mechanism of Laminaria japonica polysaccharide intervention on radiation-induced brain injury in mice. METHODS: Ninety-six male Kunming mice, SPF grade, were randomly divided into four groups (n=24 per group): a control group, an irradiation group, a Laminaria japonica polysaccharide (LJP) group, and an irradiation+LJP group. All mice were given 7-day continuous administration before radiation, and then 60Go γ-rays (30 Gy) were used to establish a radiation brain injury model in mice. Immunofluorescence method was used to detect the expression of fibrinogen and activation and deposition of astrocytes in the brain tissue. The ultrastructural changes of the cerebrovascular system were investigated under transmission electron microscope. The motion trails of the mice in the Morris water maze were observed before and after treatment. RESULTS AND CONCLUSION: After radiotherapy, the expression of fibrinogen in brain tissue was significantly increased, and astrocytes were obviously activated, while the radiation plus LJP intervention significantly relieved the deposition of fibrinogen in mice. Under the electron microscope, the brain vascular endothelial cells swelled, the basement membrane was broken and shed, and the blood-brain barrier structure changed after radiation. However, the ultrastructural morphology of the mouse blood-brain barrier in the radiation+LJP group was similar to that of the control group. After radiation, the number of times and activity time of the mice passing through the platform area was significantly reduced in the Morris water maze test (P < 0.05), while the activities of the radiation mice in the platform area showed an increasing trend after the LJP intervention. These results suggest that the protective effect of LJP on radiation-induced brain injury may be mainly achieved by maintaining the stability of the blood-brain barrier structure and regulating its permeability. [ABSTRACT FROM AUTHOR]

Drug-induced liver injury (DILI) is one of the common adverse drug reactions and is the main cause of withdrawal of drugs after marketing, which has attracted more and more attention of the public, and herb-induced liver injury (HILI) is a special type of DILI. In recent years, the frequent occurrence of HILI not only seriously endangers the health of patients, but also causes the controversy over the safety of traditional Chinese medicine. Therefore, this article reviews the potential risk factors for HILI from the three aspects of "patient", "drug", and "use", so as to provide a basis for the objective identification, prevention, and control of HILI and a reference for the construction of traditional Chinese medicine pharmacovigilance system represented by liver injury. [ABSTRACT FROM AUTHOR]

Inflammasomes play an important role in the innate immunity of the liver; however, the excessive activation of inflammasomes can lead to liver inflammation and injury. The mechanism of nucleotide - binding oligomerization domain - like receptor protein 3 ( NLRP3) inflammasome - mediated liver injury has been extensively studied. Related studies have shown that the development of various liver diseases may be associated with the excessive activation of inflammasomes, especially NLRP3 inflammasome. This article reviews inflammasomes, the activation mechanism of NLRP3 inflammasome, and the role of NLRP3 inflammasome in different liver diseases, so as to provide a reference for the treatment targets of liver diseases from the perspective of NLRP3 inflammasome. [ABSTRACT FROM AUTHOR]

In order to clarify the influence of temperature and CO2 partial pressure on corrosion of N80 steel pipe, and to optimize and match appropriate anti-corrosion measures in combination with corrosion environment by weight loss method, the corrosion rate of materials was obtained by simulating CO2 flooding conditions with high temperature autoclave, and the types of corrosion products were determined by scanning electron microscope, EDS analysis and XRD analysis.The results show that under simulated CO2 flooding conditions, when CO2 partial pressure is 0～10 MPa, the corrosion rate of both gas and liquid phases exceed the standard requirements of 0.076 mm/a, and the main possible components of corrosion products are FeCO3 and with the increase of pressure, the average corrosion rate of N80 pipe increases at first and then decreases. With the increase of temperature, the corrosion maximum of N80 pipe appears at 80 ℃,and the corrosion product film is dense and the corrosion rate decreases at 100 ℃.Therefore, it is necessary to adopt matched corrosion inhibitor or other anti-corrosion measures to improve its safe use effect. [ABSTRACT FROM AUTHOR]