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4,193 results on '"Yi C."'

353. Lipid Nanoparticle and Liposome Reference Materials: Assessment of Size Homogeneity and Long-Term −70 °C and 4 °C Storage Stability

Author

Jakubek, Zygmunt J., Chen, Sam, Zaifman, Josh, Tam, Yuen Yi C., and Zou, Shan

Abstract

With recent advances and anticipated proliferation of lipid nanoparticle (LNP)-delivered vaccines and therapeutics, there is a need for the availability of internationally recognized reference materials of LNP systems. Accordingly, we developed six LNP and liposome (anionic, neutral, and cationic each) candidate reference material formulations and thoroughly characterized by dynamic light scattering their particle hydrodynamic size (Z-avr) and polydispersity. We also evaluated the particle size homogeneity and long-term −70 °C and 4 °C storage stability using multiple large sets of randomly selected vials for each formulation. The formulations stored at −70 °C remained stable and homogeneous for a minimum of 9 months. The Z-avr relative combined uncertainty and the long-term variability were both <1.3% for liposome formulations and anionic LNPs, (3.9% and 1.7%) for neutral LNPs, and (6.7% and 4.4%) for cationic LNPs. An inadvertent few-hour-long storage temperature increase to −35 °C due to a freezer malfunction resulted in a small change of the size and size distribution of anionic liposomes and LNPs but, unexpectedly, a larger size increase of the neutral and cationic liposomes (≤5%) and LNPs (≤25%). The mean Z-avr values of the LNPs stored at 4 °C appeared to slowly increase with t1/3, where tis the storage time, and the Z-avr between-vial heterogeneity and mean polydispersity index values appeared to decrease; no change was observed for liposomes. The size and size distribution evolution of LNPs stored at 4 °C was attributed to an incomplete equilibration of the formulations following the addition of sucrose prior to the initial freezing. Such a process of size increase and size distribution narrowing has not been previously discussed nor observed in the context of LNPs.

Published
2023
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354. Exosomes as Targeted Delivery Drug System: Advances in Exosome Loading, Surface Functionalization and Potential for Clinical Application

Author

Guo, Zun Y., Tang, Yue, and Cheng, Yi C.

Abstract

Exosomes are subtypes of vesicles secreted by almost all cells and can play an important role in intercellular communication. They contain various proteins, lipids, nucleic acids and other natural substances from their metrocytes. Exosomes are expected to be a new generation of drug delivery systems due to their low immunogenicity, high potential to transfer bioactive substances and biocompatibility. However, exosomes themselves are not highly targeted, it is necessary to develop new surface modification techniques and targeted drug delivery strategies, which are the focus of drug delivery research. In this review, we introduced the biogenesis of exosomes and their role in intercellular communication. We listed various advanced exosome drug-loading techniques. Emphatically, we summarized different exosome surface modification techniques and targeted drug delivery strategies. In addition, we discussed the application of exosomes in vaccines and briefly introduced milk exosomes. Finally, we clarified the clinical application prospects and shortcomings of exosomes.

Published
2023
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355. YHR150w and YDR479c encode peroxisomal integral membrane proteins involved in the regulation of peroxisome number, size, and distribution in Saccharomyces cerevisiae

Author

Vizeacoumar, Franco J., Torres-Guzman, Juan C., Tam, Yuen Yi C., Aitchison, John D., and Rachubinski, Richard A.

Subjects
Brewer's yeast -- Research, Cytology -- Research, Biological sciences
Abstract

The peroxin Pex24p of the yeast Yarrowia lipolytica exhibits high sequence similarity to two hypothetical proteins, Yhr150p and Ydr479p, encoded by the Saccharomyces cerevisiae genome. Like Y/Pex24p, both Yhr150p and Ydr479p have been shown to be integral to the peroxisomal membrane, but unlike Y/Pex24p, their levels of synthesis are not increased upon a shift of cells from glucose- to oleic acid-containing medium. Peroxisomes of cells deleted for either or both of the YHR150w and YDR479c genes are increased in number, exhibit extensive clustering, are smaller in area than peroxisomes of wild-type cells, and often exhibit membrane thickening between adjacent peroxisomes in a cluster. Peroxisomes isolated from cells deleted for both genes have a decreased buoyant density compared with peroxisomes isolated from wild-type cells and still exhibit clustering and peroxisomal membrane thickening. Overexpression of the genes PEX25 or VPS1, but not the gene PEX11, restored the wild-type phenotype to cells deleted for one or both of the YHR150w and YDR479c genes. Together, our data suggest a role for Yhr150p and Ydr479p, together with Pex25p and Vps1p, in regulating peroxisome number, size, and distribution in S. cerevisiae. Because of their role in peroxisome dynamics, YHR150w and YDR479c have been designated as PEX28 and PEX29, respectively, and their encoded peroxins as Pex28p and Pex29p.

Published
2003

374. LC/MS-Based Global Metabolomic Identification of Serum Biomarkers Differentiating Hepatocellular Carcinoma from Chronic Hepatitis B and Liver Cirrhosis

Author

Pan, Hong Y., primary, Wu, Qing Q., additional, Yin, Qiao Q., additional, Dai, Yi N., additional, Huang, Yi C., additional, Zheng, Wei, additional, Hui, Tian C., additional, Chen, Mei J., additional, Wang, Ming S., additional, Zhang, Jia J., additional, Huang, Hai J., additional, and Tong, Yong X., additional

Published
2021
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381. A deep convolutional neural network enabled pelvic ultrasound imaging algorithm for early and accurate diagnosis of ovarian cancer

Author

Gao, Y., primary, Zeng, S., additional, Xiong, X., additional, Cai, G., additional, Wang, Z., additional, Xu, X., additional, Chi, J., additional, Jiao, X., additional, Liu, J., additional, Li, R., additional, Yao, S., additional, Li, X., additional, Song, K., additional, Tang, J., additional, Xing, H., additional, Yu, Z., additional, Zhang, Q., additional, Yi, C., additional, Kong, B., additional, Xie, X., additional, Ma, D., additional, and Gao, Q., additional

Published
2020
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395. Set-theoretic estimation based on A Priori knowledge of the noise distribution

Author

Kuo, Chjng J., Deller, J.R. Jr., Lin, Chen Y., and Tsai, Yi C.

Subjects
Algorithms -- Usage, Noise -- Research, Signal processing -- Research, Business, Computers, Electronics, Electronics and electrical industries
Abstract

A new algorithm to estimate linear in parameters models is presented.

Published
2000

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