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The Geostationary Lightning Mapper (GLM) on the Geostationary Operational Environmental Satellite 16 (GOES-16) detects total lightning continuously, with a high spatial resolution and detection efficiency. Coincident data from the GLM and the Advanced Baseline Imager (ABI) are used to explore the correlation between the cloud top properties and flash activity across the continental United States (CONUS) sector from May to September 2020. A large number of collocated infrared (IR) brightness temperature (TBB), cloud top height (CTH) and lightning data provides robust statistics. Overall, the likelihood of lightning occurrence and high flash density is higher if the TBB is colder than 225 K. The higher CTH is observed to be correlated with a larger flash rate, a smaller flash size, stronger updraft, and larger optical energy. Furthermore, the cloud top updraft velocity (w) is estimated based on the decreasing rate of TBB, but it is smaller than the updraft velocity of the convective core. As a result, the relationship between CTH and lightning flash rate is investigated independently of w over the continental, oceanic and coastal regimes in the tropics and mid-latitudes. When the CTH is higher than 12 km, the flash rates of oceanic lightning are 38% smaller than those of both coastal and continental lightning. In addition, it should be noted that more studies are necessary to examine why the oceanic lightning with low clouds (CTH < 8 km) has higher flash rates than lightning over land and coast. Finally, the exponents of derived power relationship between CTH and lightning flash rate are smaller than four, which is underestimated due to the GLM detection efficiency and the difference between IR CTH and 20 dBZ CTH. The results from combining the ABI and GLM products suggest that merging multiple satellite datasets could benefit both lightning activity and parameterization studies, although the parallax corrections should be considered.

Neuroinflammation is a pathological event associated with many neurological disorders, including dementia and stroke. The choroid plexus (ChP) is a key structure in the ventricles of the brain that secretes cerebrospinal fluid (CSF), forms a blood-CSF barrier, and responds to disease conditions by recruiting immune cells and maintaining an immune microenvironment in the brain. Despite these critical roles, the exact structural and functional changes to the ChP over post-stroke time remain to be elucidated. We induced ischemic stroke in C57BL/6J mice via transient middle cerebral artery occlusion which led to reduction of cerebral blood flow and infarct stroke. At 1-7 days post-stroke, we detected time-dependent increase in the ChP blood-CSF barrier permeability to albumin, tight-junction damage, and dynamic changes of SPAK-NKCC1 protein complex, a key ion transport regulatory system for CSF production and clearance. A transient loss of SPAK protein complex but increased phosphorylation of the SPAK-NKCC1 complex was observed in both lateral ventricle ChPs. Most interestingly, stroke also triggered elevation of proinflammatory Lcn2 mRNA and its protein as well as infiltration of anti-inflammatory myeloid cells in ChP at day 5 post-stroke. These findings demonstrate that ischemic strokes cause significant damage to the ChP blood-CSF barrier, contributing to neuroinflammation in the subacute stage. [ABSTRACT FROM AUTHOR]

In age-related macular degeneration (AMD), hydroquinone (HQ)-induced oxidative damage in retinal pigment epithelium (RPE) is believed to be an early event contributing to dysregulation of inflammatory cytokines and vascular endothelial growth factor (VEGF) homeostasis. However, the roles of antioxidant mechanisms, such as autophagy and the ubiquitin-proteasome system, in modulating HQ-induced oxidative damage in RPE is not well-understood. This study utilized an in-vitro AMD model involving the incubation of human RPE cells (ARPE-19) with HQ. In comparison to hydrogen peroxide (H2O2), HQ induced fewer reactive oxygen species (ROS) but more oxidative damage as characterized by protein carbonyl levels, mitochondrial dysfunction, and the loss of cell viability. HQ blocked the autophagy flux and increased proteasome activity, whereas H2O2 did the opposite. Moreover, the lysosomal membrane-stabilizing protein LAMP2 and cathepsin D levels declined with HQ exposure, suggesting loss of lysosomal membrane integrity and function. Accordingly, HQ induced lysosomal alkalization, thereby compromising the acidic pH needed for optimal lysosomal degradation. Pretreatment with MG132, a proteasome inhibitor and lysosomal stabilizer, upregulated LAMP2 and autophagy and prevented HQ-induced oxidative damage in wildtype RPE cells but not cells transfected with shRNA against ATG5. This study demonstrated that lysosomal dysfunction underlies autophagy defects and oxidative damage induced by HQ in human RPE cells and supports lysosomal stabilization with the proteasome inhibitor MG132 as a potential remedy for oxidative damage in RPE and AMD.

The structure and evolution of the atmospheric planetary boundary layer (PBL) plays an important role in the physical and chemical processes of cloud–radiation interaction, vertical mixing and pollutant transport in the atmosphere. The PBL parameterization scheme describes the vertical transport of atmospheric momentum, heat, water vapor and other physical quantities in the boundary layer. The accuracy of wind field simulation and prediction is one of the most significant parameters in the field of atmospheric science and wind energy. Limited by the observation data, there are few studies on wind energy development. A 3D Doppler wind LiDAR (DWL) providing the high-vertical-resolution wind data over the urban complex underlying surface in February 2018 was employed to systematically evaluate the accuracy of vertical wind field simulation for the first time. 11 PBL schemes of the Weather Research and Forecasting Model (WRF) were employed in simulation. The model results were evaluated in groups separated by weather (sunny days, hazy days and windy days), observation height layers of wind field, and various observation wind speeds. Among these factors, the simulation accuracy is most closely related to the observation height layers of wind field. The simulation is fairly accurate at a height of 1000–2000 m, as most of the relative mean biases for wind speed and wind direction are less than 20% and 6% respectively. Below 1000 m, the wind speed and direction biases are about 30–150% m·s−1 and 6–30%, respectively. Moreover, when the observed wind speed was lower than 5 m·s−1, the biases were usually large, and the wind speed relative mean bias reaches up to 50–300%. In addition, the accuracy of the simulated wind profile is better in the range of 10–15 m·s−1 than other speed ranges, and is better above 1000 m than below 1000 m in the boundary layer. We see that the WRF boundary layer schemes have different applicabilities to different weather conditions. The WRF boundary layer schemes have significant differences in wind field simulations, with larger error under the complex topographies. A PBL scheme is not likely to maintain its advantages in the long term under different conditions including altitude and weather conditions.

This paper determines the cost-benefit levels of yellow croaker farming households by comparing and analysing responses to questionnaires distributed to 405 households located in nine breeding regions of three counties (cities) in the Chinese provinces of Fujian and Zhejiang. A transcendental logarithmic stochastic frontier production function model is used to perform a quantitative analysis on the large yellow croaker production technique efficiency of different forms of industry organisation, with the farming household as the basic production unit. The findings indicate that the average production technology efficiency of the farming households is 84.33% and room for improvement remains; and there are significant differences between the production technique efficiency of farming households from different regions and under different forms of organisation. An analysis of the factors that affect farming household production technique efficiency found that capital input has a greater effect than labour input. Family participation and the number of individuals involved in the rearing of large yellow croakers, participation in technical training, and joining a closely-knit industry organisation all have a significant positive effect on increasing the production technique efficiency. These empirical results provide a basis and practical reference for optimising the development of the large yellow croaker farming industry, enhancing the degree of closeness of the organisations in the industry, and improving aquaculture performance.

Abstract Background To provide an overview of phantom limb pain (PLP) in China. This includes the prevalence of PLP and possible risk factors. Methods In a retrospective study, telephone interviews were conducted with 391 amputation patients who underwent extremity amputations at a tertiary hospital in China. Results PLP was found in 29% of the amputees. Pre-amputation pain (OR = 10.4, P = 0.002) and postoperative analgesia (OR = 4.9, P = 0.008) were identified as high-risk factors for PLP. 82.1% of PLP patients experienced pre-amputation pain. The average pain intensity of PLP was 5.1 ± 2.2, with 31.9% having severe intensity. The effects of PLP on the quality of the PLP patients were as follows: 7.8% of the patients had to limit their daily life and 29.0% of the patients had to limit their social activities. 17.3 and 25.7% of patients experienced depression and sleeping disorder respectively, while 18.9% had loss of interest and even 16.1% of PLP patients had attempted suicide. No effective treatments were found in 78.9% of these patients. Conclusions PLP has markedly affected the lives of patients. Pre-amputation pain and postoperative epidural analgesia might be risk factors for the phantom limb pain after amputation. Prevention of pre-amputation pain and sudden post-amputation deafferentation should be recommended to the amputees.

Rapeseed (Brassica napus L.) is the largest oilseed crop in China and accounts for about 20% of world production. For the last 10 years, the production, planting area, and yield of rapeseed have been stable, with improvement of seed quality and especially seed oil content. China is among the leading countries in rapeseed genomic research internationally, having jointly with other countries accomplished the whole genome sequencing of rapeseed and its two parental species, Brassica oleracea and Brassica rapa. Progress on functional genomics including the identification of QTL governing important agronomic traits such as yield, seed oil content, fertility regulation, disease and insect resistance, abiotic stress, nutrition use efficiency, and pod shattering resistance has been achieved. As a consequence, molecular markers have been developed and used in breeding programs. During 2005–2014, 215 rapeseed varieties were registered nationally, including 210 winter- and 5 spring-type varieties. Mechanization across the whole process of rapeseed production was investigated and operating instructions for all relevant techniques were published. Modern techniques for rapeseed field management such as high-density planting, controlled-release fertilizer, and biocontrol of disease and pests combined with precision tools such as drones have been developed and are being adopted in China. With the application of advanced breeding and production technologies, in the near future, the oil yield and quality of rapeseed varieties will be greatly increased, and more varieties with desirable traits, especially early maturation, high yield, high resistance to biotic and abiotic stress, and suitability for mechanized harvesting will be developed. Application of modern technologies on the mechanized management of rapeseed will greatly increase grower profit.

Abstract Background Conventional moxibustion is a representative non-drug intervention in traditional Chinese medicine, and it has been reported to produce encouraging results and benefits in relieving symptoms and improving the quality of life for patients with knee osteoarthritis (KOA) in previous clinical trials and systematic reviews. Given that increasing concerns on the safety of generated smoke from conventional moxibustion have received much attention, smoke-free moxibustion is regarded as a potential alternative. However, whether smoke-free moxibustion would display a similar efficacy to that of conventional moxibustion still remains unclear. Therefore, this randomized controlled trial attempts to investigate the difference of efficacy between conventional moxibustion and smoke-free moxibustion in patients with KOA. Methods/design This is a multicenter, randomized, single-blinded, parallel-group clinical trial. A total of 138 eligible participants with KOA will be randomly allocated to two groups (conventional moxibustion group and smoke-free moxibustion group) in seven hospitals in China. Participants will receive 12 sessions of moxibustion treatment at three acupoints (EX-LE4, ST35, and ST36) over a period of 4 weeks (3 sessions per week). A smoke-removing device is placed at the top of the moxibustion device for the smoke-free moxibustion group (n = 69), while the conventional moxibustion group (n = 69) is treated with traditional moxibustion. The primary outcome measure will be the change of the global scale of the Western Ontario and McMaster Universities Osteoarthritis Index (WOMAC) from the baseline to 4 weeks. Secondary outcomes include the visual analog scale VASand Patient Global Assessment scores. Follow-up measurements will be performed on the 8th and 12th weeks after random allocation. Discussion This study will contribute to providing a solid foundation for the selection of moxibustion in clinical application as well as future research in moxibustion therapy. Trial registration ClinicalTrials.gov, NCT02772055 . Registered on 12 May 2016.

As a typical material of the third-generation semiconductor with wide band gap, high thermal conductivity and high critical breakdown electric field, silicon carbide(SiC) has a huge potential in the applications of photoelectric devices that can work in some extreme conditions, such as in a high temperature or intense radiation environment. Here, the research status of luminescence properties and applications of SiC was summarized. The preparation methods and characteristic luminescence of monocrystal, nanocrystalline and thin film of SiC were presented. Besides, the progress and prospect of SiC luminescence control was also discussed in this paper. Utilizing the emerging technologies, we will be able to modify SiC's properties like luminescence spectrum and efficiency.

The trackless rubber tyred vehicle (TRTV) is an important transporting equipment of underground coal mine auxiliary transportation system in the world. Presently, most TRTVs are driven by flameproof diesel engine. However, few researches were focused on their dynamic performance in the past. In this paper, the characterizing parameters and influencing factors of the dynamic performance of a flameproof diesel engine driven TRTV were summarized and discussed firstly. Then the basic dynamic equations of the TRTV were established. Finally, the dynamic performances of the WCQ-3B type TRTV were simulated by ADVISOR. And some important dynamic performance parameters such as maximum speed, acceleration time and maximum gradability were discussed by simulation. What is more, the fuel economy and emission performance of the vehicle were also analysed. It is shown that the dynamic performance of WCQ-3B type TRTV can satisfy the design and usage requirements of underground coal mine.

This paper deals with the fabrication of an inductorless wideband low-noise amplifier (LNA). The LNA includes two branches in parallel: a common-source (CS) path and a common-gate (CG) path. The CS path is responsible for providing enough power gain, while the CG path is used to achieve the input impedance matching. To eliminate the noise contribution of the CG path, the noise cancellation technique is applied. Therefore, the overall noise figure (NF) is improved. The phase mismatch between the two paths is also quantitatively analyzed to investigate its effect on gain and NF. The analytical results agree well with the simulation results. The LNA has been fabricated by a commercial 0.18-μm CMOS process. The measurement results show that the LNA has achieved a maximum gain of 14.5 dB with 1.7-GHz 3-dB gain bandwidth and a minimum NF of 3 dB. The tested input 1-dB gain compression point (IP1 dB) is -10.4 dBm at 1 GHz and the input third-order intercept point is 0.25 dBm. With 1.8-V supply, the LNA draws only 6-mA dc current.

Spermatozoa are one of the few mammalian cell types that cannot be fully derived in vitro, severely limiting the application of modern genomic techniques to study germ cell biology. The current gold standard approach of characterizing single-gene knockout mice is slow as generation of each mutant line can take 6–9 months. Here, we describe an in vivo approach to rapid functional screening of germline genes based on a new nonsurgical, nonviral in vivo transfection method to deliver nucleic acids into testicular germ cells. By coupling multiplex transfection of short hairpin RNA (shRNA) constructs with pooled amplicon sequencing as a readout, we were able to screen many genes for spermatogenesis function in a quick and inexpensive experiment. We transfected nine mouse testes with a pilot pool of RNA interference (RNAi) against well-characterized genes to show that this system is highly reproducible and accurate. With a false negative rate of 18% and a false positive rate of 12%, this method has similar performance as other RNAi screens in the well-described Drosophila model system. In a separate experiment, we screened 26 uncharacterized genes computationally predicted to be essential for spermatogenesis and found numerous candidates for follow-up studies. Finally, as a control experiment, we performed a long-term selection screen in neuronal N2a cells, sampling shRNA frequencies at five sequential time points. By characterizing the effect of both libraries on N2a cells, we show that our screening results from testis are tissue-specific. Our calculations indicate that the current implementation of this approach could be used to screen thousands of protein-coding genes simultaneously in a single mouse testis. The experimental protocols and analysis scripts provided will enable other groups to use this procedure to study diverse aspects of germ cell biology ranging from epigenetics to cell physiology. This approach also has great promise as an applied tool for validating diagnoses made from medical genome sequencing, or designing synthetic biological sequences that can act as potent and highly specific male contraceptives.

High power lasers can produce electron-positron pairs at GeV energies, but doing so through laser–laser collisions would require exceedingly high intensities. Here the authors present an all-optical scheme for pair production by irradiating near-critical-density plasmas with two counter-propagating lasers.

Millions every day purchase their raw meat in wet markets around the globe, especially in Hong Kong city, where modern and a traditional way of living is made possible. While food hygiene standards in Hong Kong have more recently focused on the safety of meat sold in these wet markets, the hygienic surface level of wooden cutting boards used for processing meats is seldom observed. This original study performed microbial community profiling, as well as isolating and identifying various strains multiple wooden cutting boards from nine wet markets located on Hong Kong Island. Our study also investigated the efficiency of scraping the surface of cutting boards as a traditional cleaning technique in Hong Kong. Results indicate that these hygienic practices are inefficient for guarantying proper surface hygiene as some most tested cutting boards were found to harbor microbial species typically associated with hospital nosocomial infections, such as Klebsiella pneumoniae. Further analysis also led to discovering the presence of antibiotic-resistant genes (ARGs) among isolated strains. Our results showcase the significance and effects of cross-contamination in Hong Kong wet markets, especially with regards to the potential spreading of clinically-relevant strains and ARGs on food processing surfaces. This study should, therefore, serve as a basis to review current hygienic practices in Hong Kong’s wet market on a larger scale, thereby improving food safety and ultimately, public health.

Primate studies indicate that the pyramidal tract (PyT) could originate from Brodmann area (BA) 6. However, in humans, the accurate origin of PyT from BA 6 is still uncertain owing to difficulties in visualizing anatomical features such as the fanning shape at the corona radiata and multiple crossings at the semioval centrum. High angular-resolution diffusion imaging (HARDI) could reliably replicate these anatomical features. We explored the origin of the human PyT from BA 6 using HARDI. With HARDI data of 30 adults from the Massachusetts General Hospital-Human Connectome Project (MGH-HCP) database and the HCP 1021 template (average of 1021 HCP diffusion data), we visualized the PyT at the 30-averaged group level and the 1021 large-sample level and validated the observations in each of the individuals. Endpoints of the fibers within each subregion were quantified. PyT fibers originating from the BA 6 were consistently visualized in all images. Specifically, the bilateral supplementary motor area (SMA) and dorsal premotor area (dPMA) were consistently found to contribute to the PyT. PyT fibers from BA 6 and those from BA 4 exhibited a twisting topology. The PyT contains fibers originating from the SMA and dPMA in BA 6. Infarction of these regions or aging would result in incomplete provision of information to the PyT and concomitant decreases in motor planning and coordination abilities.

Induction of oxidative damage by the activation of histone deacetylase (HDAC) is an integral event that causes major membrane damage of ocular tissues and leads to the pathogenesis of cataract. It is elucidated that nuclear factor-κB is a mediator in the process of cataract development. However, studies on the role played by epigenetic proteins in regulating cataract pathogenesis are limited. Hence, in the current investigation, ARPE-19 human retinal epithelial cells were used as an experimental model to elucidate the role of HDAC inhibition and its mechanism behind the cataract pathogenesis. ARPE-19 cells were exposed to H 2 O 2 , with and without Trichostatin A (TSA), a pan-HDAC inhibitor, and maintained along with control cells without any treatment. On exposure to H 2 O 2 , cells were susceptible to oxidative stress as it is evident from the reduced expression levels of superoxide dismutase (SOD), catalase, and GSH levels. Simultaneously, H 2 O 2 -exposed cells showed the nuclear translocation of NF-κB with the activation of inflammatory cytokines such as CXCL1 and IL-6. In addition, the mRNA expression analysis revealed that the GADD45α, COX-2, MCP-1, and ICAM-1 expressions were increased in H 2 O 2 group. Moreover, the activity of HDAC was increased to 2-fold with a significant reduction in the histone acetyltransferase (HAT) activity in cells that were maintained under oxidative conditions. However, TSA was able to inhibit the critical cytokines’ expression with attenuated HDAC activity and limited NF-κB translocation. Furthermore, pre-treatment of TSA significantly suppressed the transcript levels of up-regulated inflammatory markers in cells. Together, these findings offer new insight into the role of HDACs in regulating cellular processes involved in the pathogenesis of cataract as well as the potential use of HDAC inhibitors as therapeutics for controlling the disease progression.

Pulmonary endometriosis is a rare form of thoracic endometriosis. We herein describe a 29-year-old woman with recurrent hemoptysis associated with her menstrual cycle. The patient had a 4-month history of catamenial hemoptysis without thoracic pain, respiratory embarrassment, cough, fever, night sweating, or loss of appetite. Chest computed tomography revealed exudation shadows in the right lower pulmonary lobe and small fiber lesions in the right middle lobe and left lung. Thoracoscopic wedge resection of the right lower pulmonary lobe was performed, and the pathological result was pulmonary endometriosis. No evidence of hemoptysis during menstruation was found following the operation.

An improved multifilamentary conduction model for the reset process in resistive random access memory (RRAM) is constructed by considering the stochastic distribution of oxygen vacancies (Vo). In this context, conduction filaments (CFs) have different Vo densities and diffusion barriers. Fully coupled multiphysics simulations of RRAM with three CFs are performed using the time domain finite difference method to self-consistently solve the current transport, heat conduction, and Vo transport equations. The simulated I-V characteristics agree well with experiment. Since the three CFs have different thermal diffusion barriers, as in previous studies, they rupture at different applied voltages. Evolution of each CF in the reset process is investigated: from when its electrical conductivity is linearly dependent to when it is exponentially dependent on Vo density.