372 results on '"Wen-wen Zhang"'
Search Results
352. Enhanced expression of Cx43 and gap junction communication in vascular smooth muscle cells of spontaneously hypertensive rats.
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LI-JIE WANG, WEI-DONG LIU, LIANG ZHANG, KE-TAO MA, LEI ZHAO, WEN-YAN SHI, WEN-WEN ZHANG, YING-ZI WANG, LI LI, and JUN-QIANG SI
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GAP junctions (Cell biology) ,VASCULAR smooth muscle ,MUSCLE cells ,CONNEXINS ,MESENTERIC artery ,REVERSE transcriptase polymerase chain reaction ,THERAPEUTICS ,HYPERTENSION - Abstract
Niflumic acid (NFA) is a novel gap junction (GJ) inhibitor. The aim of the present study was to investigate the effect of NFA on GJ communication and the expression of connexin (Cx) in vascular smooth muscle cells (VSMCs) of mesenteric arterioles of spontaneously hypertensive rats (SHR). Whole-cell patch clamp recording demonstrated that NFA at 1x10-4 M significantly inhibited the inward current and its effect was reversible. The time for charging and discharging of cell membrane capacitance (C
input ) reduced from 9.73 to 0.48 ms (P<0.05; n=6). Pressure myograph measurement showed that NFA at 3x10-4 M fully neutralized the contraction caused by phenylephrine. The relaxation responses of normotensive control Wistar Kyoto (WKY) rats were significantly higher, compared with those of t he SHRs ( P<0.05; n=6). Western blot and reverse transcription-quantitative polymerase chain reaction analyses showed that the mRNA and protein expression levels of Cx43 of the third-level branch of mesenteric arterioles of the SHRs and WKY rats were higher, compared with those of the first-level branch. The mRNA and protein expression levels of Cx43 of the primary and third-level branches of the mesenteric arterioles in the SHRs were higher, compared with those in the WKY rats (P<0.05; n=6). The mRNA levels of Cx43 in the mesenteric arterioles were significantly downregulated by NFA in a concentration-dependent manner (P<0.01; n=6). The protein levels of Cx43 in primary cultured VSMCs isolated from the mesenteric arterioles were also significantly downregulated by NFA in a concentration-dependent manner (P<0.01; n=6). These results showed that the vasorelaxatory effects of GJ inhibitors were reduced in the SHRs, which was associated with a higher protein expression level of Cx43 in the mesenteric arterioles of the SHRs. NFA also relaxed the mesenteric arterioles by reducing the expression of Cx43, which decreased blood pressure. Therefore, regulation of the expression of GJs may be a therapeutic target for the treatment of hypertension. [ABSTRACT FROM AUTHOR]- Published
- 2016
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353. A New Convenient Reduction of Aralkyl Ketones to Alcohols Using Raney Nickel-Ammonium Formate
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Wen-Wen Zhang, Fen-Er Chem, Heng Zhang, and Wei Yuan
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Reduction (complexity) ,chemistry.chemical_compound ,Chemistry ,Yield (chemistry) ,Organic Chemistry ,Ammonium formate ,Organic chemistry ,Raney nickel - Abstract
Various aralkyl ketones have been reduced to their corresponding alcohols in high yield using Raney Ni-HCO2NH4
- Published
- 1991
354. Caragana Aboveground Biomass and Area Relationship in Semiarid Loess Plateau Region
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Bai, Dong-Mei, primary, Guo, Zhong-Sheng, additional, ., Man-Cai Guo, additional, ., Ting Ning, additional, and ., Wen-Wen Zhang, additional
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- 2013
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355. PD-L1 is upregulated by EBV-driven LMP1 through NF-κB pathway and correlates with poor prognosis in natural killer/T-cell lymphoma.
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Xi-wen Bi, Hua Wang, Wen-wen Zhang, Jing-hua Wang, Wen-jian Liu, Zhong-jun Xia, Hui-qiang Huang, Wen-qi Jiang, Yu-jing Zhang, and Liang Wang
- Subjects
T-cell lymphoma ,CYTOTOXIC T cells ,LYMPHOMAS ,DIAGNOSIS ,PROGNOSIS - Abstract
Background: Natural killer/T-cell lymphoma (NKTCL) is an Epstein-Barr virus (EBV)-associated, highly aggressive lymphoma. Treatment outcome remains sub-optimal, especially for advanced-stage or relapsed diseases. Programmed cell death receptor 1 (PD-1) and PD ligand 1 (PD-L1) have become promising therapeutic targets for various malignancies, but their role in the pathogenesis and their interactions with EBV in NKTCL remains to be investigated. Methods: Expression of PD-L1 was measured in NK-92 (EBV-negative) and SNK-6 (EBV-positive) cells by western blot, quantitative real-time PCR and enzyme-linked immunosorbent assay, and flow cytometry, respectively. Latent membrane protein 1 (LMP1)-harboring lentiviral vectors were transfected into NK-92 cells to examine the correlation between LMP1 and PD-L1 expression. Proteins in the downstream pathways of LMP1 signaling were measured in NK-92 cells transfected with LMP1-harboring or negative control vectors as well as in SNK-6 cells. PD-L1 expression on tumor specimens and serum concentration of soluble PD-L1 were collected in a retrospective cohort of patients with Ann Arbor stage I~II NKTCL, and their prognostic significance were analyzed. Results: Expression of PD-L1 was significantly higher in SNK-6 cells than in NK-92 cells, at both protein andmRNA levels. Expression of PD-L1 was remarkably upregulated in NK-92 cells transfected with LMP1-harboring lentiviral vectors compared with those transfected with negative control vectors. Proteins in the MAPK/NF-κB pathway were upregulated in LMP1-expressing NK-92 cells compared with the negative control. Selective inhibitors of those proteins induced significant downregulation of PD-L1 expression in LMP1-expressing NK-92 cells as well as in SNK-6 cells. Patients with a high concentration of serum soluble PD-L1 (≥3.4 ng/ml) or with a high percentage of PD-L1 expression in tumor specimens (≥38 %) exhibited significantly lower response rate to treatment and remarkably worse survival, compared with their counterparts. A high concentration of serum soluble PD-L1 and a high percentage of PD-L1 expression in tumor specimens were independent adverse prognostic factors among patients with stage I~II NKTCL. Conclusions: PD-L1 expression positively correlated LMP1 expression in NKTCL, which was probably mediated by the MAPK/NF-κB pathway. PD-L1 expression in serum and tumor tissues has significant prognostic value for early-stage NKTCL. [ABSTRACT FROM AUTHOR]
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- 2016
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356. Simultaneous Determination of Amitraz and its Metabolites in Blood by Support Liquid Extraction Using UPHLC-QTOF.
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Xue Gao, Hao Guo, Wen-wen Zhang, and Chaokang Gu
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ACARICIDES ,METABOLITES ,LIQUID chromatography-mass spectrometry ,BLOOD testing ,POISONING ,TANDEM mass spectrometry ,LIQUID-liquid extraction ,FORENSIC toxicology - Abstract
This paper describes the use of UPHLC-QTOF for the analysis of pesticide amitraz and its metabolites in human blood. UPHLC-QTOF working in full scan mode simultaneously with a single MS/MS scan at 10 eV was employed to analyze in solid-support liquid-liquid extracts (SLE) of the human blood. Detection and quantification were carried out using full scan data (protonated molecules [M+H]+) while MS/MS data were used for fragment identification only. MS/MS scans were not found to have any negative influence on quantitation under the applied conditions. Verification studies were accomplished at low concentrations (1 ng/mL), and accuracy errors lower than 5 ppm were achieved. The human blood extracts spiked at LOQ and three QC fortification levels produced average recoveries in the range of 79.3-92.5% with relative standard deviations smaller than 10% for all analytes. Limits of detection (LODs) were between 0.1 and 0.5 ng/mL. Finally, the proposed method was successfully applied to a case of human poisoning. [ABSTRACT FROM AUTHOR]
- Published
- 2016
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357. 4-Tosyl-1-oxa-4-azaspiro[4.5]deca-6,9-dien-8-one
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Ping Yin, Hai-Bin Yin, Ling He, Wen-Wen Zhang, and Wencai Huang
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chemistry.chemical_compound ,Crystallography ,Tosyl ,chemistry ,Perpendicular ,General Materials Science ,General Chemistry ,Crystal structure ,Dihedral angle ,Condensed Matter Physics ,Ring (chemistry) ,Organic Papers ,Deca - Abstract
In the mol-ecule of the title compound, C(15)H(15)NO(4)S, the two six-membered rings are almost parallel to each other [dihedral angle = 1.87 (9)°] and perpendicular to the mean plane through the five-membered ring [dihedral angles of 89.98 (10) and 89.04 (10)°]. The crystal structure is stabilized by inter-molecular C-H⋯O hydrogen-bonding inter-actions.
- Published
- 2009
358. JNK mediates the effects of oxytocin microinjected into the paraventricular nucleus on gastric ischemia-reperfusion in rats
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Wen-Wen Zhang, Yong-Mei Zhang, and Jian-Fu Zhang
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medicine.medical_specialty ,medicine.anatomical_structure ,Endocrinology ,Oxytocin ,business.industry ,Internal medicine ,Ischemia ,medicine ,medicine.disease ,business ,Nucleus ,medicine.drug - Published
- 2009
359. Novel Soluble Dietary Fiber-Tannin Self-Assembled Film: A Promising Protein Protective Material.
- Author
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Guo-Bin Song, Juan Xu, Hua Zheng, Ying Feng, Wen-Wen Zhang, Kun Li, Shuang-shuang Ge, Kai Li, and Hong Zhang
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- 2015
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360. Lower irritation microemulsion-based rotigotine gel: formulation optimization and in vitro and in vivo studies.
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Zheng Wang, Hong-Jie Mu, Xue-Mei Zhang, Peng-Kai Ma, Sheng-Nan Lian, Feng-Pu Zhang, Sheng-Ying chu, Wen-Wen Zhang, Ai-Ping Wang, Wen-Yan Wang, and Kao-Xiang sun
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- 2015
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361. Formation control for multiple mobile robots based on the Spiking Neural Network.
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Xu Wang, Zhi-Qiang Cao, Wen-Wen Zhang, Min Tan, Zeng-Guang Hou, and Xiu-Qing Wang
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- 2010
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362. [Untitled]
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Eszter Somogyi, Brendan J. Frey, Mark D. Robinson, Benjamin J. Blencowe, Richard Chang, Ofer Shai, Eric Sat, Eftekhar Eftekharpour, Janet Rossant, Benoit G. Bruneau, Wen-Wen Zhang, Nicholas Mitsakakis, Nancy Laurin, Qun Pan, Quaid Morris, Malina A. Bakowski, Jane E. Aubin, Jörg Grigull, Derek van der Kooy, Ralph A Zirngibl, Michael G. Fehlings, Nevan J. Krogan, Wen-Tao Peng, Naveed Mohammad, Timothy P. Hughes, and Jack Greenblatt
- Subjects
Genetics ,Regulation of gene expression ,0303 health sciences ,Pair-rule gene ,Genomics ,Biology ,General Biochemistry, Genetics and Molecular Biology ,Gene expression profiling ,03 medical and health sciences ,0302 clinical medicine ,Gene cluster ,Gene expression ,General Agricultural and Biological Sciences ,Gene ,Functional genomics ,030217 neurology & neurosurgery ,030304 developmental biology - Abstract
Large-scale quantitative analysis of transcriptional co-expression has been used to dissect regulatory networks and to predict the functions of new genes discovered by genome sequencing in model organisms such as yeast. Although the idea that tissue-specific expression is indicative of gene function in mammals is widely accepted, it has not been objectively tested nor compared with the related but distinct strategy of correlating gene co-expression as a means to predict gene function. We generated microarray expression data for nearly 40,000 known and predicted mRNAs in 55 mouse tissues, using custom-built oligonucleotide arrays. We show that quantitative transcriptional co-expression is a powerful predictor of gene function. Hundreds of functional categories, as defined by Gene Ontology 'Biological Processes', are associated with characteristic expression patterns across all tissues, including categories that bear no overt relationship to the tissue of origin. In contrast, simple tissue-specific restriction of expression is a poor predictor of which genes are in which functional categories. As an example, the highly conserved mouse gene PWP1 is widely expressed across different tissues but is co-expressed with many RNA-processing genes; we show that the uncharacterized yeast homolog of PWP1 is required for rRNA biogenesis. We conclude that 'functional genomics' strategies based on quantitative transcriptional co-expression will be as fruitful in mammals as they have been in simpler organisms, and that transcriptional control of mammalian physiology is more modular than is generally appreciated. Our data and analyses provide a public resource for mammalian functional genomics.
- Published
- 2004
363. Bismuthoxyiodide Nanoflakes/Titania Nanotubes Arrayed p-n Heterojunction and Its Application for Photoelectrochemical Bioanalysis.
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Wei-Wei Zhao, Zhao Liu, Shu Shan, Wen-Wen Zhang, Jing Wang, Zheng-Yuan Ma, Jing-Juan Xu, and Hong-Yuan Chen
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TITANIUM dioxide ,TITANIUM oxide nanotubes ,HETEROJUNCTIONS ,PHOTOELECTROCHEMICAL cells ,CANCER diagnosis ,VASCULAR endothelial growth factors ,PHOTOELECTROCHEMISTRY - Abstract
We have developed sensitive detection of cancer biomarker vascular endothelial growth factor (VEGF) using the p-n heterojunction comprised of p-type BiOI nanoflakes (NFs) array and n-type TiO
2 nanotubes (NTs) array. Due to the unique arrayed structure and the synergy effect of photoelectrochemistry in the formed p-n junction, the synthesized configuration has superior excitation efficiency and thus excellent photoresponsibility. Then, the fabricated p-n heterojunction was integrated with an exquisite bioassay protocol for addressing VEGF using a sandwich immunoassay with glucosedehydrogenase (GDH) as the enzyme tags. Due to the excellent performance of BiOI NFs array/TiO2 NTs array and the ingenious signaling mechanism, the proposed system could achieve the sensitive and specific VEGF detection. This work not only presents a simple BiOI NFs array/TiO2 NTs array p-n heterojunction for general applications in the broad photochemistry areas, but also opens a different horizon for current development of advanced PEC biomolecular detection. [ABSTRACT FROM AUTHOR]- Published
- 2014
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364. Preparation of novel mcl-PHA by green synthesis: influence of active small molecule via esterification effect on side chain.
- Author
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Kun Li, Wen-Wen Zhang, Xiao-Juan Shi, Lan-Xiang Liu, Kai Li, Juan Xu, Jin-Ju Ma, and Hong Zhang
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- 2019
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365. PD-L1 is upregulated by EBV-driven LMP1 through NF-κB pathway and correlates with poor prognosis in natural killer/T-cell lymphoma
- Author
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Liang Wang, Wen Jian Liu, Wen Qi Jiang, Zhong Jun Xia, Wen Wen Zhang, Yu Jing Zhang, Hua Wang, Jing Hua Wang, Xi Wen Bi, and Hui Qiang Huang
- Subjects
0301 basic medicine ,Male ,Herpesvirus 4, Human ,Cancer Research ,Biopsy ,medicine.disease_cause ,Transfection ,lcsh:RC254-282 ,B7-H1 Antigen ,Flow cytometry ,Programmed cell death receptor 1 ,Epstein–Barr virus ,Viral Matrix Proteins ,03 medical and health sciences ,0302 clinical medicine ,Downregulation and upregulation ,Western blot ,PD-L1 ,Latent membrane protein 1 ,medicine ,Humans ,Molecular Biology ,Cells, Cultured ,medicine.diagnostic_test ,biology ,lcsh:RC633-647.5 ,Research ,Natural killer/T-cell lymphoma ,NF-kappa B ,lcsh:Diseases of the blood and blood-forming organs ,Hematology ,Middle Aged ,lcsh:Neoplasms. Tumors. Oncology. Including cancer and carcinogens ,medicine.disease ,Natural killer T cell ,Prognosis ,Lymphoma ,Gene Expression Regulation, Neoplastic ,Lymphoma, Extranodal NK-T-Cell ,030104 developmental biology ,Oncology ,030220 oncology & carcinogenesis ,Host-Pathogen Interactions ,biology.protein ,Cancer research ,Female - Abstract
Background Natural killer/T-cell lymphoma (NKTCL) is an Epstein–Barr virus (EBV)-associated, highly aggressive lymphoma. Treatment outcome remains sub-optimal, especially for advanced-stage or relapsed diseases. Programmed cell death receptor 1 (PD-1) and PD ligand 1 (PD-L1) have become promising therapeutic targets for various malignancies, but their role in the pathogenesis and their interactions with EBV in NKTCL remains to be investigated. Methods Expression of PD-L1 was measured in NK-92 (EBV-negative) and SNK-6 (EBV-positive) cells by western blot, quantitative real-time PCR and enzyme-linked immunosorbent assay, and flow cytometry, respectively. Latent membrane protein 1 (LMP1)-harboring lentiviral vectors were transfected into NK-92 cells to examine the correlation between LMP1 and PD-L1 expression. Proteins in the downstream pathways of LMP1 signaling were measured in NK-92 cells transfected with LMP1-harboring or negative control vectors as well as in SNK-6 cells. PD-L1 expression on tumor specimens and serum concentration of soluble PD-L1 were collected in a retrospective cohort of patients with Ann Arbor stage I~II NKTCL, and their prognostic significance were analyzed. Results Expression of PD-L1 was significantly higher in SNK-6 cells than in NK-92 cells, at both protein and mRNA levels. Expression of PD-L1 was remarkably upregulated in NK-92 cells transfected with LMP1-harboring lentiviral vectors compared with those transfected with negative control vectors. Proteins in the MAPK/NF-κB pathway were upregulated in LMP1-expressing NK-92 cells compared with the negative control. Selective inhibitors of those proteins induced significant downregulation of PD-L1 expression in LMP1-expressing NK-92 cells as well as in SNK-6 cells. Patients with a high concentration of serum soluble PD-L1 (≥3.4 ng/ml) or with a high percentage of PD-L1 expression in tumor specimens (≥38 %) exhibited significantly lower response rate to treatment and remarkably worse survival, compared with their counterparts. A high concentration of serum soluble PD-L1 and a high percentage of PD-L1 expression in tumor specimens were independent adverse prognostic factors among patients with stage I~II NKTCL. Conclusions PD-L1 expression positively correlated LMP1 expression in NKTCL, which was probably mediated by the MAPK/NF-κB pathway. PD-L1 expression in serum and tumor tissues has significant prognostic value for early-stage NKTCL. Electronic supplementary material The online version of this article (doi:10.1186/s13045-016-0341-7) contains supplementary material, which is available to authorized users.
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366. Prediction of 5-year overall survival of diffuse large B-cell lymphoma on the pola-R-CHP regimen based on 2-year event-free survival and progression-free survival.
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Wan-Ru Zhang, Xin Liu, Qiu-Zi Zhong, Tao Wu, Yong Yang, Bo Chen, Hao Jing, Yuan Tang, Jing Jin, Yue-Ping Liu, Yong-Wen Song, Hui Fang, Ning-Ning Lu, Ning Li, Yi-Rui Zhai, Wen-Wen Zhang, Shu-Lian Wang, Fan Chen, Lin Yin, and Shu-Nan Qi
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DIFFUSE large B-cell lymphomas , *PROGRESSION-free survival , *OVERALL survival , *REGRESSION analysis , *STATISTICAL correlation - Abstract
This study aimed to predict the 5-year overall survival (OS) benefit of pola-R-CHP versus R-CHOP in the POLARIX trial based on the 2-year event-free survival (EFS) and progression-free survival (PFS) rates in diffuse large B-cell lymphoma (DLBCL). We identified randomized controlled trials (RCT) published before 31 May 2023. The correlation between the logarithmic (log) hazard ratio (HR) for EFS (HREFS) or PFS (HRPFS) and the HR for OS (HROS) was estimated at the triallevel. Correlation analysis was performed between 2-year PFS or EFS and 5-year OS rates at the treatment arm-level. Linear regression models were used to calculate the 5-year OS of pola-R-CHP and R-CHOP. In the included 20 RCTs, a linear correlation between HREFS (r=0.765) or HRPFS (r=0.534) and HROS was observed at the trial- level. Two-year EFS (r=0.918) or 2-year PFS (r=0.865) correlated linearly with 5-year OS. Linear regression analysis between 2-year EFS/PFS and 5-year OS gave estimated 5-year OS rates between pola-R-CHP and R-CHOP of 6.4% and 6.3%, respectively. Two-year EFS and PFS are feasible early endpoints in patients with DLBCL treated primarily with immunochemotherapy. The polaR-CHP regimen is expected to improve 5-year OS. [ABSTRACT FROM AUTHOR]
- Published
- 2024
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367. Correlation of Metabolic Indexes as Predictors with Obstructive Sleep Apnea
- Author
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WEN Wen, ZHANG Kainan, CHEN Yulan, LI Yu, ZHANG Xiangyang
- Subjects
sleep apnea, obstructive ,dyslipidemia ,metabolic indices ,chinese visceral adiposity index ,forecasting ,case-control studies ,Medicine - Abstract
Background Obstructive sleep apnea (OSA) has a high prevalence, and it has been shown to be an independent risk factor for various diseases. Therefore, it is important to strengthen screening for population at highrisk of OSA. OSA patients are prone to combine with lipid metabolism disorders, but it remains unclear whether the atherogenic index of plasma (AIP), visceral adiposity index (VAI), lipid accumulation product (LAP), cardiometabolic index (CMI), and Chinese visceral adiposity index (CVAI), which are used asmetabolic indexes, can be used to predict OSA. Objective To analyze the correlation between metabolic indexes and OSA, and evaluate the predictive efficacy of each metabolic index through a case-control study. Methods A total of 2 968 inpatients with suspected OSA and aged ≥18 years who completed polysomnography (PSG) in the First Affiliated Hospital of Xinjiang Medical University from March 2017 to June 2022 were selected, with 2 850 patients finally included based on the inclusion and exclusion criteria and divided into the OSA group 〔apnea-hypopnea index (AHI) ≥5 times/h, n=2 193〕 and non-OSA group (AHI0.05), but AIP and CVAI were associated with OSA (P
- Published
- 2023
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368. Material Selection and Evaluation on Internal Coating of Associated Gas Pipeline in H2S-CO2-O2 Coexistence System in Tahe Oilfield
- Author
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XIAO Wen-wen, ZHANG Wen-bo, LIN De-yun, GE Peng-li, XU Yan-yan, LIAO Ke-xi, YANG-Na
- Subjects
h2s-co2-o2 coexistence system ,associated gas pipeline ,pipeline material selection ,internal coating evaluation ,Materials of engineering and construction. Mechanics of materials ,TA401-492 ,Technology - Abstract
To study the corrosion behavior of three commonly used steels in associated gas pipelines in the three-gas coexistence system of H2S-CO2-O2 can provide guidance for the corrosion prevention and control of associated gas pipelines in Tahe oilfield.Based on the typical corrosion working conditions in Tahe oilfield and taking 20 steel,L245NS steel and 3Cr steel as the research objects,the corrosion rates and corrosion products of the three steels under typical working conditions in H2S - CO2- O2 coexistence system were studied through high -temperature and high-pressure reactor experiment.Results showed that the corrosion rate of 20# steel was the highest,followed by L245NS steel,and the corrosion rate of 3Cr steel was the lowest.The corrosion products of the three pipes were mainly Fe,FeS,FeCO3 and Fe2O3.The corrosion resistance behaviors of four typical internal anti-corrosion coatings in Tahe oilfield were studied through an adhesion test and the weight gain method that involved the measuring of the weight gain before and after corrosion,and the results indicated that coating 2 possessed the optimal corrosion resistance and adhesion.Considering the cost performance,“L245NS+coating 2”was finally selected as the anti-corrosion measure for the associated gas pipelines in Tahe Oilfield.
- Published
- 2023
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369. TXNIP reduces pro-proliferation effect of GLP-1 on mouse islet β cells
- Author
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ZHANG Wen-ting, WANG Jin, YUE Ji-ping, JIN Wen-wen, ZHANG Zhi-nan, JIAO Xiang-ying
- Subjects
thioredoxin interacting protein ,glp-1 ,glp-1 receptor ,cell proliferation ,Medicine - Abstract
Objective To observe the role of thioredoxin interacting protein (TXNIP) in the proliferation of islet β cells promoted by Glucagon-like peptide 1 (GLP-1). Methods The mice were divided into db/m group and db/db group; TXNIP over-expression (Ad-TXNIP-GFP) MIN6 cells were constructed by stably transfected with lentivirus, which were divided into control group, Ad-GFP group and Ad-TXNIP-GFP group. Western blot was used to detect the expression of TXNIP and proliferating cell nuclear antigen (PCNA) in pancreatic tissues of db/m and db/db mice. The morphology of the islets, the number of β cells, GLP-1R and Ki67 were observed by HE and immunohistochemistry method. The expression of GLP-1 in serum was detected by ELISA. The level of GLP-1R protein in pancreatic tissue was detected by immunohistochemistry. Results Islet structure was destructed, the number of β cells and PCNA expression decreased in pancreatic tissue of db/db mice (P<0.05). The level of GLP-1 in serum reduced (P<0.05), and the level of GLP-1 receptor in pancreatic tissue and islet was reduced (P<0.05). The expression of TXNIP in pancreatic tissues of db/db mice increased (P<0.05). The TXNIP overexpression cell model was successfully constructed (P<0.05), and the level of GLP-1 receptor decreased in islet β cells (P<0.05). The overexpression of TXNIP reduced the proliferative effect of islet β cells to GLP-1 (P<0.05). Conclusions TXNIP acts on the GLP-1 receptor to reduce the proliferation of islet β cells promoted by GLP-1.
- Published
- 2020
370. CXCL13 drives spinal astrocyte activation and neuropathic pain via CXCR5.
- Author
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Bao-Chun Jiang, De-Li Cao, Xin Zhang, Zhi-Jun Zhang, Li-Na He, Chun-Hua Li, Wen-Wen Zhang, Xiao-Bo Wu, Berta, Temugin, Ru-Rong Ji, Yong-Jing Cao, Jiang, Bao-Chun, Cao, De-Li, Zhang, Xin, Zhang, Zhi-Jun, He, Li-Na, Li, Chun-Hua, Zhang, Wen-Wen, Wu, Xiao-Bo, and Ji, Ru-Rong
- Subjects
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ASTROCYTES , *SPINE physiology , *JOINT pain , *CHEMOKINES , *B cells , *PHYSIOLOGY , *CELL metabolism , *RNA metabolism , *ANIMAL experimentation , *CELL receptors , *CELLS , *CELLULAR signal transduction , *CYTOKINES , *MICE , *NEURALGIA , *RESEARCH funding , *RNA , *SPINAL cord - Abstract
Recent studies have implicated chemokines in microglial activation and pathogenesis of neuropathic pain. C-X-C motif chemokine 13 (CXCL13) is a B lymphocyte chemoattractant that activates CXCR5. Using the spinal nerve ligation (SNL) model of neuropathic pain, we found that CXCL13 was persistently upregulated in spinal cord neurons after SNL, resulting in spinal astrocyte activation via CXCR5 in mice. shRNA-mediated inhibition of CXCL13 in the spinal cord persistently attenuated SNL-induced neuropathic pain. Interestingly, CXCL13 expression was suppressed by miR-186-5p, a microRNA that colocalized with CXCL13 and was downregulated after SNL. Spinal overexpression of miR-186-5p decreased CXCL13 expression, alleviating neuropathic pain. Furthermore, SNL induced CXCR5 expression in spinal astrocytes, and neuropathic pain was abrogated in Cxcr5-/- mice. CXCR5 expression induced by SNL was required for the SNL-induced activation of spinal astrocytes and microglia. Intrathecal injection of CXCL13 was sufficient to induce pain hypersensitivity and astrocyte activation via CXCR5 and ERK. Finally, intrathecal injection of CXCL13-activated astrocytes induced mechanical allodynia in naive mice. Collectively, our findings reveal a neuronal/astrocytic interaction in the spinal cord by which neuronally produced CXCL13 activates astrocytes via CXCR5 to facilitate neuropathic pain. Thus, miR-186-5p and CXCL13/CXCR5-mediated astrocyte signaling may be suitable therapeutic targets for neuropathic pain. [ABSTRACT FROM AUTHOR]
- Published
- 2016
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371. CX3CL1-mediated macrophage activation contributed to paclitaxel-induced DRG neuronal apoptosis and painful peripheral neuropathy.
- Author
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Zhen-Zhen Huang, Dai Li, Cui-Cui Liu, Yu Cui, He-Quan Zhu, Wen-Wen Zhang, Yong-Yong Li, and Wen-Jun Xin
- Subjects
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CHEMOKINES , *MACROPHAGE activation , *APOPTOSIS , *PACLITAXEL , *DRUG side effects , *TREATMENT of peripheral neuropathy , *CANCER chemotherapy , *CANCER patients - Abstract
Painful peripheral neuropathy is a dose-limiting side effect of paclitaxel therapy, which hampers the optimal clinical management of chemotherapy in cancer patients. Currently the underlying mechanisms remain largely unknown. Here we showed that the clinically relevant dose of paclitaxel (3×8mg/kg, cumulative dose 24mg/kg) induced significant upregulation of the chemokine CX3CL1 in the A-fiber primary sensory neurons in vivo and in vitro and infiltration of macrophages into the dorsal root ganglion (DRG) in rats. Paclitaxel treatment also increased cleaved caspase-3 expression, induced the loss of primary afferent terminal fibers and decreased sciatic-evoked A-fiber responses in the spinal dorsal horn, indicating DRG neuronal apoptosis induced by paclitaxel. In addition, the paclitaxel-induced DRG neuronal apoptosis occurred exclusively in the presence of macrophage in vitro study. Intrathecal or systemic injection of CX3CL1 neutralizing antibody blocked paclitaxel-induced macrophage recruitment and neuronal apoptosis in the DRG, and also attenuated paclitaxel-induced allodynia. Furthermore, depletion of macrophage by systemic administration of clodronate inhibited paclitaxel-induced allodynia. Blocking CX3CL1 decreased activation of p38 MAPK in the macrophage, and inhibition of p38 MAPK activity blocked the neuronal apoptosis and development of mechanical allodynia induced by paclitaxel. These findings provide novel evidence that CX3CL1-recruited macrophage contributed to paclitaxel-induced DRG neuronal apoptosis and painful peripheral neuropathy. [ABSTRACT FROM AUTHOR]
- Published
- 2014
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372. Influential Factors on Activated Sludge Deterioration in Anoxic-Oxic (A/O) Biological Treatment of Coking Wastewater.
- Author
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Chun-hui Zhang, Hai-dong Hu, Jun Chen, Wen-wen Zhang, Yuan-jie Guo, and Ke Ning
- Subjects
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ACTIVATED sludge process , *ENVIRONMENTAL degradation , *BIOLOGICAL treatment of water , *WASTEWATER treatment , *PH effect , *BIOLOGICAL reagents , *SODIUM hydroxide , *HIGH temperatures - Abstract
The control measures for deterioration of aerobic activated sludge were experimented. Wastewater samples with different pH, temperature, SVI, and MLSS were investigated to detect the cause of sludge deterioration. The results show that after being operated at high S2O32- (which could lead to a pH decrease) loading in an A/O biological reactor treated for coking wastewater, though the pH was controlled at about 7.0 by the addition of NaOH, the COD removal efficiency was decreased deeply. The MLSS decreased from 3,800-4,300 mg/L to 2,020 mg/L after loading of S2O32- and NaOH in the oxic unit of A/O biological reactor for 12 days. The COD removal efficiencies and MLSS concentrations were decreased sharply when the wastewater's temperature was over 30ºC, which indicates the bulking of activated sludge in the oxic unit of A/O biological reactor. The results suggest that it cannot inhibit the occurrence of sludge deterioration only by controlling the pH in the oxic unit of an A/O biological reactor. Although the addition of NaOH could inhibit sludge bulking, the decreased MLSS concentration would significantly reduce the COD removal efficiency. The decline of COD removal efficiency may be due to the high temperature leading to sludge death. [ABSTRACT FROM AUTHOR]
- Published
- 2013
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