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The 76 amino acid N-terminal proB-type natriuretic peptide (NT-proBNP) is proposed for evaluating and monitoring heart pathologies characterized by myocardial wall stress. Strenuous exercise might generate transitory ischemia, myocardial stress, and diastolic left ventricular dysfunction, possibly inducing an increase of some biochemical parameter concentrations. An alert has been claimed owing to biochemical and instrumental signs of heart dysfunction in recreational athletes during marathon races. We studied the behaviour of NT-proBNP in 15 mountain marathoners before and after a race. The concentrations of the parameter were lower than that observed in controls at rest and were similar to that observed in professional soccer and rugby players. The concentrations significantly increased after the race. NT-proBNP is low at rest in professional athletes, and the increase after physical exercise is physiological. The marathoners, even when performing races in a high-altitude environment, show NT-proBNP concentrations similar to those of athletes from other sports disciplines, characterized by low levels of effort and by a mix of aerobic and anaerobic metabolism. The increase of NT-proBNP is linked to strenuous physical exercise and to heavy heart effort, testified also by an increase of troponin I. However, the role of the NT-proBNP could be important to screen recreational and professional marathoners to avoid possible heart problems and sudden cardiac death in subjects with occult heart disease. The results of the present study are relevant to the design and evaluation of training programs for improving strength and function of professional marathoners.

Protein expression changes induced in thioglycolate-elicited peritoneal murine macrophages (M Phi) by infection with type III Group B Streptococcus (GBS) are described. Proteins from control M Phi and M Phi incubated 2 h with live or heat-inactivated GBS were separated by 2-DE. Proteins whose expression was significantly different in infected M Phi, as compared with control cells, were identified by MS/MS analysis. Changes in the expression level of proteins involved in both positive and negative modulation of phagocytic functions, stress response and cell death were induced in M Phi by GBS infection. In particular, expression of enzymes playing a key role in production of reactive oxygen species was lowered in GBS-infected M Phi. Significant alterations in the expression of some metabolic enzymes were also observed, most of the glycolytic and of the pentose-cycle enzymes being down-regulated in M Phi infected with live GBS. Finally, evidence was obtained that GBS infection affects the expression of enzymes or enzyme subunits involved in ATP synthesis and in adenine nucleotides interconversion processes.

Background Recent evidence indicates that various types of interactions between nervous and immune system are important in pathogenesis of depression. These findings show that a significant role in developing depression play pro-inflammatory cytokines that may mediate its psychological, and neurobiological manifestations. Great importance among these cytokine molecules plays interleukin-6 (IL-6). There is growing evidence that this inflammatory process related to depression may be influenced by psychological stress as well as organic inflammatory conditions. These findings suggest that specific influences related to traumatic stress and dissociation could be found in close relationship to increased level of cytokine IL-6. Methods In the present study we have performed psychometric measurement of depression (BDI-II), traumatic stress symptoms (TSC-40) and dissociation (DES, SDQ-20), and immunochemical measure of serum IL-6 in 40 inpatients with unipolar depression (mean age 42.3 ± 6.8). Results The results show that IL-6 is significantly correlated to BDI-II (Spearman R = 0.47, p R = 0.32, p R = 0.34, p R = 0.25, p = 0.11). Conclusion The findings of the present study indicate that increased level of IL-6 in depression could be directly related to symptoms of traumatic stress and somatoform dissociation.

Cytauxzoonosis, caused by the protozoan parasite, Cytauxzoon felis, is a tick-borne disease of domestic cats causing high mortality. The reservoir is wild felids. In this study, 7 archived cases of the disease were examined through in situ hybridization for localization of the parasite and by immunohistochemistry for various cell markers to characterize infected cells. The riboprobe used was specific for the 16S-like rRNA subunit of Babesia microti, which shares 91% identity with the same gene for C. felis. In situ hybridization highlighted the presence of the organism in several tissues, most prominently lung and spleen, and, in general, there were 2 to 10 times more infected cells seen with in situ hybridization than with HE. Parasite-laden cells were usually found within vessels. These cells were often tightly packed and frequently formed parasitic thrombi. Immunohistochemistry with an antilysozyme antibody confirmed the macrophage origin of the infected cells. Using an antibody specific for calprotectin (Mac387), parasitized cells were markedly devoid of this protein, which may explain the lack of diapedesis and vascular crowding of parasitized cells, providing more circulating parasites for the tick vector. Immunohistochemical labeling for 2 proliferation markers, proliferating cell nuclear antigen (PCNA) and p53, indicated that parasitized cells have a heightened replicative ability, which is probably an additional parasite-driven modification to facilitate survival and transmission.

Recent findings suggest that specific deficits in neural synchrony and binding may underlie cognitive disturbances in schizophrenia and that key aspects of schizophrenia pathology involve discoordination and disconnection of distributed processes in multiple cortical areas associated with cognitive deficits. In the present study we aimed to investigate the underlying cortical mechanism of disturbed frontal-temporal-central-parietal connectivity in schizophrenia by examination of the synchronization patterns using wavelet phase synchronization index and coherence between all defined couples of 8 EEG signals recorded at different cortical sites in its relationship to positive and negative symptoms of schizophrenia. 31 adult schizophrenic outpatients with diagnosis of paranoid schizophrenia (mean age 27.4) were assessed in the study. The obtained results present the first quantitative evidence indicating direct relationship between wavelet phase synchronization and coherence in pairs of EEG signals recorded from frontal, temporal, central and parietal brain areas and positive and negative symptoms of schizophrenia. The performed analysis demonstrates that the level of phase synchronization and coherence in some pairs of EEG signals is inversely related to positive symptoms, negative symptoms and general psychopathology in temporal scales (frequency ranges) given by wavelet frequencies (WFs) equal to or higher than 7.56 Hz, and positively related to negative symptoms in wavelet frequencies equal to or lower than 5.35 Hz. This finding suggests that higher and lower frequencies may play a specific role in binding and connectivity and may be related to decreased or increased synchrony with specific manifestation in cognitive deficits of schizophrenia.

The thermal dehydration process of fibroferrite, FeOH(SO4)·5H2O, a secondary iron-bearing hydrous sulfate, was investigated by in situ high-temperature synchrotron X-ray powder diffraction (HT-XRPD), in situ high-temperature Fourier transform infrared spectroscopy (HT-FTIR) and thermal analysis (TGA-DTA) combined with evolved gas mass spectrometry. The data analysis allowed the determination of the stability fields and the reaction paths for this mineral as well as characterization of its high-temperature products. Five main endothermic peaks are observed in the DTA curve collected from room T up to 800 °C. Mass spectrometry of gases evolved during thermogravimetric analysis confirms that the first four mass loss steps are due to water emission, while the fifth is due to a dehydroxylation process; the final step is due to the decomposition of the remaining sulfate ion. The temperature behavior of the different phases occurring during the heating process was analyzed, and the induced structural changes are discussed. In particular, the crystal structure of a new phase, FeOH(SO4)·4H2O, appearing at about 80 °C due to release of one interstitial H2O molecule, was solved by ab initio real-space and reciprocal-space methods. This study contributes to further understanding of the dehydration mechanism and thermal stability of secondary sulfate minerals.

BRAF, a serine/threonine kinase of the RAF family, is a downstream transducer of the RAS-regulated MAPK pathway. V600E mutation of BRAF protein is the most common genetic alteration occurring in papillary thyroid carcinomas and is prognostic of poor clinicopathological outcomes. Protein expression in the subclass of PTC bearing the BRAF(V600E) mutation was investigated by using 2-DE and MS/MS techniques and compared to that of matched normal thyroid tissues from seven patients. 2-D gel image analysis revealed that the expression of eight polypeptide spots, corresponding to five proteins, were significantly underexpressed in PTC bearing BRAF(V600E) mutation whereas 25 polypeptides, representing 19 distinct proteins, were significantly upregulated in tumour tissue, as compared to normal thyroid. Among the differentially expressed polypeptides, mitochondrial proteins, ROS-scavenger enzymes, apoptosis-related proteins as well as proteins involved in tumour cell proliferation were identified. Although dissimilarities between the present results and those previously reported can be ascribed to the use of different 2-DE techniques, the possibility that BRAF(V600E) mutation is responsible for changes in protein expression distinct from those induced by other oncogenes cannot be ruled out.

Narcolepsy with cataplexy (NC) and narcolepsy without cataplexy (NwoC) are lifelong neurological disorders characterized primarily by excessive daytime sleepiness. Emotional events such as laughter are a trigger of cataplexy in NC.We compared the volumes of key limbic structures, the amygdala and hippocampus, in 53 NC, 23 NwoC and 37 control subjects. MRI volumetry was performed in FreeSurfer (FS) and by manual delineation.We found no differences in amygdalar volume in the three groups, however, hippocampal volume was significantly smaller in the NC group than in other groups. Amygdalar and hippocampal volumes assessed by FS were significantly greater, but strong positive correlation between manual and FS results were observed. Thus, both methods are suitable for amygdalar and hippocampal volumetry.

Reported brain abnormalities in anatomy and function in psychiatric and neurological patients led to a project based on qualitative electroencephalography examination and analysis in an attempt to find specific brain derived pattern--or sequence of brain locations involved in processing various stimuli--both visual and auditory.Specialized software called Brain Activation Sequences was built according to our team member specifications (M.S.). The software utilizes event related potentials recorded during cognitive/emotion processing in participants (healthy controls, neurological patients and psychiatric patients) to calculate the sequence of brain areas using nonlinear and linear algorithms.Results show significant differences in activation patterns between patients and healthy controls as well as significant similarities within the groups of patients and controls in both performed testing experiments.

Strains of Newcastle disease virus (NDV) have different abilities to elicit neurologic signs. To determine the capacity of different NDV strains to replicate and cause lesions in the brain, independently of their peripheral replication, 1-day-old chickens were inoculated in the subdural space with 7 NDV strains of different virulence (4 velogenic, 2 mesogenic, 1 lentogenic). Velogenic strains induced severe necrotizing and heterophilic ventriculitis and meningitis, as well as edema of the neuroparenchyma, and replicated extensively in the nervous tissue by day 2 postinfection, as demonstrated by immunohistochemistry, when all infected birds died. Clinical signs, microscopic lesions, and viral replication were delayed (days 3 and 4 postinfection) with mesogenic strains. Velogenic and mesogenic NDV strains replicated mainly in neurons, and immunolabeling was first detected in surface-oriented areas (periventricular and submeningeal), possibly as a reflection of the inoculation route. The lentogenic NDV strain did not cause death of infected birds; replication was confined to the epithelium of the ependyma and choroid plexuses; and lesions consisted of lymphoid aggregates limited to the choroid plexuses. Results show that extensive NDV replication in the brain is typical of velogenic and mesogenic, but not lentogenic, NDV strains. In addition, this study suggests that differences in the rate of NDV replication in nervous tissue, not differences in neurotropism, differentiate velogenic from mesogenic NDV strains. This study indicates that intracerebral inoculation might be used as an effective method to study the mechanisms of NDV neuropathogenesis.

Background In mammals, interleukin 2 (IL-2) has been shown to decrease replication or attenuate pathogenicity of numerous viral pathogens (herpes simplex virus, vaccinia virus, human respiratory syncytial virus, human immunodeficiency virus) by activating natural killer cells (NK), cytotoxic T lymphocytes and expanding subsets of memory cells. In chickens, IL-2 has been shown to activate T cells, and as such it might have the potential to affect replication and pathogenesis of Newcastle disease virus (NDV). Methods To assess the effect of IL-2 during NDV infection in chickens, we produced a recombinant virulent NDV strain expressing chicken IL-2 (rZJ1-IL2). The effects of IL-2 expression were investigated in vivo using the intracerebral pathogenicity index (ICPI) in day-old chicks and pathogenesis experiments in 4-week-old chickens. In these studies, rZJ1-IL2 was compared to a control virus expressing the green fluorescent protein (rZJ1-GFP). Assessed parameters included survival curves, detailed histological and immunohistochemical grading of lesions in multiple organs, and virus isolation in blood, spleen and mucosal secretions of infected birds. Results At the site of infection (eyelid), expression of IL-2 was demonstrated in areas of rZJ-IL2 replication, confirming IL-2 production in vivo. Compared to rZJ1-GFP strain, rZJ1-IL2 caused milder lesions and displayed decreased viral load in blood, spleen and mucosal secretions of infected birds. In the rZJ1-IL2-infected group, virus level in the blood peaked at day 4 post-infection (pi) (103.46 EID50 /0.1 ml) and drastically decreased at day 5 pi (100.9 EID50/0.1 ml), while in the rZJ1-GFP-infected group virus levels in the blood reached 105.35 EID50/0.1 ml at day 5. However, rZJ1-IL2-infected groups presented survival curves similar to control birds infected with rZJ1-GFP, with comparable clinical signs and 100 % mortality. Further, expression of IL-2 did not significantly affect the ICPI scores, compared to rZJ1-GFP strain. Conclusions Increased expression of chicken IL-2 during virulent NDV replication in naïve chickens decreased viral titers in blood, spleens, oral and cloacal secretions on day 4–5 post infection. This is consistent with the previously described role of IL-2 in enhancing the clearance of viruses in mammals, such as human respiratory syncytial virus. Electronic supplementary material The online version of this article (doi:10.1186/s12985-015-0353-x) contains supplementary material, which is available to authorized users.

A closed form design of narrowest possible bandpass finite impulse response filters is presented. The underlaying generating polynomial has equiripple behaviour in two separate intervals. A novel degree equation is introduced. One example demonstrates the functionality of the design procedure.

Cardiopulmonary exercise testing is a non-invasive method widely used to monitor various physiological signals, describing the cardiac and respiratory response of the patient to increasing workload. Since this method is physically very demanding, innovative data analysis techniques are needed to predict patient response thus lowering body stress and avoiding cardiopulmonary overload. This paper proposes the Cardiopulmonary Response Prediction (CRP) framework for early predicting the physiological signal values that can be reached during an incremental exercise test. The learning phase creates different models tailored to specific conditions (i.e., single-test and multiple-test models). Each model can be exploited in the real-time stream prediction phase to periodically predict, during the test execution, signal values achievable by the patient. Experimental results on a real dataset showed that CRP prediction is performed with a limited and acceptable error.

Prostasomes are vesicles secreted by prostate epithelial cells and are found in abundance in the semen. Here we characterized two different prostasome populations isolated from human seminal fluid. Prostasomes were isolated using differential centrifugation, while dynamic light scattering (DLS) was used to characterize their size and size distribution. Their protein content was analyzed using two-dimensional electrophoresis and mass spectrometry. DLS showed two distinct prostasome subpopulations in centrifuged seminal plasma, with an average hydrodynamic radius of 80 and 300 nm. The larger population was isolated after centrifugation at 20,000 × g (P20), while the smaller one was recovered at 100,000 × g (P100). The two fractions had a similar lipid composition, showing an elevated content of sphingomyelin and cholesterol. The P100 vesicles showed a significant over-expression of proteins involved in the endosomal sorting complexes required for transport (ESCRT) machinery such as Alix, TSG101, and syntenin-1. Some proteins possibly involved in prostate cancer were present only in one specific population (TMPRSS2 in P100 and VCP in P20). The different size and protein profile in the two subpopulations of prostasomes might support differential roles of the semen vesicles toward the target cells, and/or different secretion pathways from the organ of origin.

An innovative six-meter long prototype of the magnetic levitation suspended cryogen transfer line has design, constructed and tested to demo potentials of extending many missions by saving cryogens, or reducing the overall launch mass to accomplish a given mission. The use of magnetic levitation by permanent magnets (PM) and high temperature superconductors (HTS) provides support without mechanical contact and thus, the conduction part of the heat leak can be reduced to zero, which is ultimately the most serious heat leak after thermal radiation has been minimized. The configurations of HTS & PM, the levitation force in different arrangements, the mechanical design of the transfer line, the support/release structures, and the cryogenic testing results are also discussed.

The Rare Isotope Accelerator uses 805 MHz superconducting rf cavities in the linac. Power is transmitted capacitively into the cavities via a high power input coupler. The coupler was designed for greater than 10 kW cw with a VSWR less than 1.05. The design load to the 2 K liquid helium is less than 2 W. The external Q of the coupler is about 2/spl times/10/sup 7/. The couplers were conditioned off-line to over 200 kW pulsed before installation into a prototype cryomodule. The cryomodule was tested at 2 K to full accelerating gradients. Multipacting barriers in the coupler were quickly conditioned, and no arcs or discharges were observed during testing. Details of the power couplers performance will be presented.

Study design: randomized controlled before-and-after and in follow-up trial. Forty-six CAD patients volunteered to take part in the study: a group of 27 patients undertook an Intermittent Hypoxia (O2 at 10%)-Hyperoxia (O2 at 30%) Training (IHHT), while a control group (CTRL) of 19 patients was allocated to sham IHHT treatment (breathing via face mask by room air, O2 at 21%). Exercise performance, blood and metabolic profile, quality of life (MOS SF-36, Seattle Angina Questionnaire, SAQ) were measured before and after IHHT/sham IHHT in both groups; the intervention group was also assessed one month after completing the IHHT.

Overtraining syndrome (OTS) is a major concern among endurance athletes and is a leading cause in preventing them to perform for long periods. Intermittent exposure to hypoxia has been shown to be an effective way of improving performance without exercising. Aim of this pilot study was to evaluate intermittent hypoxia-hyperoxia training combined with light exercise as an intervention to facilitate athletes with OTS to restore their usual performance level. Thirty-four track and field athletes were recruited: 15 athletes with OTS volunteered to participate and undertook a conditioning programme consisting of repeated exposures to hypoxia (O2 at 10%) and hyperoxia (O2 at 30%) (6-8 cycles, total time 45 min-1 h), three times a week, delivered 1·5-2 h after a low-intensity exercise session (2 bouts of 30 min, running at 50% of VO2max with 10 min rest between bouts) over 4 weeks. Nineteen healthy track and field athletes volunteered to participate as a control group and followed their usual training schedule. Measurements before and after the intervention included exercise capacity, analysis of heart rate variability and hematological parameters. In athletes with OTS, a 4-week light exercise combined with intermittent hypoxia-hyperoxia training improved exercise performance (191·9 ± 26·9 W versus 170·8 ± 44·8 W in exercise capacity test, P = 0·01). Heart rate variability analysis revealed an improved sympatho-parasympathetic index (low frequency/high frequency ratio, 8·01 ± 7·51 before and 1·45 ± 1·71 after, P = 0·007). Hematological parameters were unchanged. Our pilot study showed that intermittent hypoxia-hyperoxia training and low-intensity exercise can facilitate functional recovery among athletes with OTS in a relatively short time.

Wakefulness of adequately long duration and quality is in every respect essential for good quality of life. Inadequately low wakefulness named excessive daytime sleepiness (EDS) is defined as a reduced ability to maintain continuous wakefulness during the day. EDS may take the form of lapses into sleep or as periods of somnolence leading to sleep onset in favorable circumstances and longer total duration of sleep within 24 h. EDS lowers the quality of life and complicates or disallows many regular activities.

Each year millions of chickens die from Newcastle disease virus (NDV) worldwide leading to severe economic and food losses. Current vaccination campaigns have limitations especially in developing countries, due to elevated costs, need of trained personnel for effective vaccine administration, and functional cold chain network to maintain vaccine viability. These problems have led to heightened interest in producing new antiviral strategies, such as RNA interference (RNAi). RNAi methodology is capable of substantially decreasing viral replication at a cellular level, both in vitro and in vivo. In this study, we utilize microRNA (miRNA)-expressing constructs (a type of RNA interference) in an attempt to target and knockdown five NDV structural RNAs for nucleoprotein (NP), phosphoprotein (P), matrix (M), fusion (F), and large (L) protein genes. Immortalized chicken embryo fibroblast cells (DF-1) that transiently expressed miRNA targeting NP mRNA, showed increased resistance to NDV-induced cytopathic effects, as determined by cell count, relative to the same cells expressing miRNA against alternative NDV proteins. Upon infection with NDV, DF-1 cells constitutively expressing the NP miRNA construct had improved cell survival up to 48 h post infection (h.p.i) and decreased viral yield up to 24 h.p.i. These results suggest that overexpression of the NP miRNA in cells and perhaps live animal may provide resistance to NDV.

Históricamente la universidad ha desarrollado la producción del conocimiento, la transmisión del saber y la investigación. Actualmente se ha constituido como una instancia de control, producción y legitimación de saberes, insertada en un contexto de tensión permanente entre lo que requieren la sociedad, el estado y el mercado. En la propuesta académica, el Plan de Estudios constituye un recorte de los campos de conocimiento seleccionados y de la estructura curricular, y los profesores determinan, mediante definiciones y acciones, cómo se transmiten, comparten y construyen conocimientos del campo disciplinar en el ámbito universitario. La enseñanza al servicio de la educación implica un particular cuidado en el proceso formativo de los estudiantes puesto que se los está preparando para ser ciudadanos críticos. En el complejo proceso de enseñanza, los conocimientos son cruzados por variables y dimensiones de orden epistemológica, psicológica, pedagógica y didáctica, cultural y social. (Fernández Sívori 2013) y del tratamiento que se haga del conocimiento se derivarán formas de implementación curricular distintas, pues de lo contrario habría una ruptura epistemológica que llevaría a la inconsistencia práctica, dando lugar a formas arbitrarias de evaluación. (Susta, Machado 2010), relación triangular conocimiento-educación-currículum en los procesos educativos que ha sido desarrollada por Álvarez Méndes (2001:31-33) Se toma el concepto de calidad educativa desarrollado por Santos Guerra (1998:90-93) y por Pruzzo (1999:9) observando que es afectada por las resoluciones que se toman acerca del currículo, los factores que inciden en el aprendizaje de los estudiantes, y el rol de los docentes, así como los aspectos organizativos y los recursos disponibles. La enseñanza de calidad facilita al estudiante la adquisición integrada de las tres dimensiones de la educación: conocimiento, sentimientos y responsabilidad moral, y capacidad de hacer. (Susta, Machado 2010) El proceso de enseñanza universitaria es complejo y hace necesaria la formación y la capacitación del docente universitario. Un caso particular son las asignaturas proyectuales en las carreras de diseño por su forma de trabajo en el aula o taller, donde se desarrollan propuestas personales del estudiante sobre un tema determinado, requiriendo del docente respuestas personalizadas correspondientes a cada caso demandando su conocimiento y pericia. (Susta 2013). Es en estas situaciones donde quedan expuestas las dificultades de los novatos (ayudantes alumnos y adscriptos) para cumplir con su función docente debido a la carencia de herramientas didácticas que faciliten la tarea de auxiliar al estudiante en su personal proceso de aprendizaje. Los procesos de transformación que se producen, negocian, evalúan y reflexionan en el aula entre estudiantes y docentes, constituyen la buena enseñanza y la visión que cada docente tiene de su labor y su objetivo de docente determina en gran medida el modo en que estructura su enseñanza, utilizando para ello las mejores habilidades y técnicas que tiene disponibles. (Susta, Machado 2010) La enseñanza universitaria, quizás por su cercanía con los campos de producción de conocimiento y por el desarrollo cognitivo de sus estudiantes, históricamente ha recurrido muy poco al conocimiento pedagógico. En la actualidad, la idea de universidad ha cambiado debido al aumento incesante del conocimiento, la creciente especialización, la masificación de la enseñanza y el aumento de los requerimientos de perfiles formativos. Estos cambios, según la visión de Feldman y Palamidessi (2001:13-14), conllevan la necesidad de un tratamiento pedagógico especializado de los problemas del curriculum y de la enseñanza universitaria y en el mismo sentido Perkins (1992:119-127) declara que la enseñanza de las asignaturas implica mucho más que aprender su contenido, dado que los estudiantes necesitan imágenes que engloben su estructura y les permitan observar cómo se ensamblan sus partes en un todo coherente. Se entiende la transferencia como la situación que permite aprender algo en un contexto determinado y luego aplicarlo en otro diferente, pero esto no se produce libremente, debe ser guiada por el docente estableciendo condiciones de aprendizaje que la propicien. Se debe verificar la transferencia de los aprendizajes y en este aspecto Pruzzo (1999:31) establece que a la evaluación “se la considera como un proceso en el que a través de medios específicos se obtiene información procedente de numerosas fuentes que permiten la interpretación de las situaciones a partir de la cual se emiten juicios y se toman las decisiones pedagógicas pertinentes”. Al plantear la delimitación conceptual de lo que se entiende por evaluación Santos Guerra (2001:9) explica que resulta deseable un modelo de evaluación y un enfoque que se centren en la comprensión y en la mejora, considerando la evaluación como un proceso de reflexión que exige a todos el compromiso con el conocimiento y con la mejora, no como ejercicio de control del evaluado. El desarrollo del proceso de observación y reflexión sobre las situaciones cotidianas del aula y la recuperación de las propias experiencias como estudiantes y su aplicación a la enseñanza tienen profunda significación pedagógica y didáctica en las que es posible reconocer las explicaciones de los docentes, las ayudas que utilizan para favorecer la comprensión, las maneras en que identifican los aprendizajes adquiridos por sus estudiantes y los modos como impacta en los docentes mismos la reflexión acerca de sus propias prácticas. (Litwin 1997, 2009) Se piensa el diseño de diversos instrumentos como aporte a la formación de novatos sin capacitación en docencia pero con sólidos conocimientos de la disciplina a enseñar, reconociendo que tanto los instrumentos propuestos como las prácticas, son abiertos, perfectibles y sujetos a cambios orientados hacia la mejora, tomando como base para el diseño de los instrumentos, fundamentos teóricos, datos aportados por investigaciones previas con docentes y estudiantes, y otros obtenidos a partir de la observación de la dinámica particular del dictado de asignaturas proyectuales. Fil: Susta, Carolina. Universidad Nacional de Mar del Plata. Facultad de Arquitectura, Urbanismo y Diseño; Argentina Fil: Machado Susseret, Néstor. Universidad Nacional de Mar del Plata. Facultad de Arquitectura, Urbanismo y Diseño; Argentina

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An outbreak of Newcastle disease (ND) in poultry was reported in Belize in 2008. The characteristics of three virulent Newcastle disease virus (NDV) isolates from this outbreak (NDV-Belize-3/08, NDV-Belize-4/08, and NDV-Belize-12/08) were assessed by genomic analysis and by clinicopathological characterization in specific-pathogen-free (SPF) chickens. The results showed that all three strains belong to NDV genotype V and are virulent, as assessed by the intracerebral pathogenicity index and the polybasic amino acid sequence at the fusion protein cleavage site. In 4-week-old SPF chickens, NDV-Belize-3/08 behaved as a typical velogenic viscerotropic NDV strain, causing severe necrohemorrhagic lesions in the lymphoid organs, with systemic virus distribution. Phylogenetic analysis of multiple NDV genotype V representatives revealed that genotype V can be divided into three subgenotypes, namely, Va, Vb, and Vc, and that all tested Belizean isolates belong to subgenotype Vb. Furthermore, these isolates are nearly identical to a 2007 isolate from Honduras and appear to have evolved separately from other contemporary viruses circulating in Mexico, clustering into a new clade within NDV subgenotype Vb.

Sleep disorders are frequent and disturbing, mostly chronic conditions. Despite the fact practice of sleep medicine is a young field. Sleep disorders were reduced to insomnia and even insomnia was not well studied until 1960ies. This article maps amazing development of sleep disorders treatment from the beginning till current date. The text covers both scientific and clinical perspectives on most frequent sleep related disorders including insomnias, hypersomnias, parasomnias, sleep related movement and breathing disorders, restless legs syndrome and circadian rhythm sleep disorders.