301. Down-regulation of miRNA-204 by LMP-1 enhances CDC42 activity and facilitates invasion of EBV-associated nasopharyngeal carcinoma cells
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Minhua Wu, Lei Ma, Xubin Deng, Gong Zhang, and Jianqing Huang
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Male ,Epstein-Barr Virus Infections ,Herpesvirus 4, Human ,Kaplan-Meier Estimate ,CDC42 ,medicine.disease_cause ,Biochemistry ,Metastasis ,Epstein–Barr virus ,Mice ,Structural Biology ,hemic and lymphatic diseases ,Latent membrane protein 1 ,Cdc42 ,cdc42 GTP-Binding Protein ,Mice, Inbred BALB C ,miR-204 ,Middle Aged ,Prognosis ,Tumor Burden ,Gene Expression Regulation, Neoplastic ,Disease Progression ,Female ,RNA Interference ,Signal Transduction ,STAT3 Transcription Factor ,Biophysics ,Down-Regulation ,Mice, Nude ,Biology ,Malignancy ,Viral Matrix Proteins ,Downregulation and upregulation ,Cell Line, Tumor ,microRNA ,Genetics ,medicine ,Carcinoma ,otorhinolaryngologic diseases ,Nasopharyngeal carcinoma ,Animals ,Humans ,Neoplasm Invasiveness ,Molecular Biology ,Binding Sites ,Base Sequence ,Nasopharyngeal Neoplasms ,Cell Biology ,Stat-3 ,medicine.disease ,MicroRNAs ,stomatognathic diseases ,Cancer research ,Neoplasm Transplantation - Abstract
Nasopharayngeal carcinoma (NPC) is an Epstein–Barr virus (EBV)-associated malignancy. It is known that microRNAs are implicated in the progression of NPC. However, the role of miR-204 in NPC is poorly understood. In this study, we found that miR-204 was down-regulated in NPC cells and tissues. Low-level expression of miR-204 was significantly associated with a more aggressive and poor prognostic phenotype of NPC. We further found that EBV-encoded latent membrane protein 1 (LMP-1) suppressed miR-204 expression by activating Stat-3. Cdc42 was identified as a direct target of miR-204. Mir-204 inhibited EBV positive C666-1 cell invasion and metastasis partly through targeting cdc42.
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