Structure solution skills in x-ray crystallography are critical capabilities for students and postdoctoral trainees in biophysics, biochemistry and structural biology. While many institutions have incorporated classes on theoretical crystallography into their curricula, practical structure solution skills are rarely imparted through coursework.We thus developed an enabling, hands-on crystallography sub-curriculum, which can be added to existing biophysics curricula that focus on the theoretical foundations. Taking place in an encouraging, collaborative learning environment, our educational intervention sets out to provide learners from diverse backgrounds with sustainable skills to tackle real-world, protein structure solution problems independently.Our curriculum is designed in a multimedia-enhanced, blended classroom format: Reading resources as well as short, narrated and pre-recorded slide presentations provide course participants ahead of class with the necessary background to work on problem sets during face-to-face sessions. Part of an evolving case study, the problem sets contain real-world diffraction datasets in various degrees of difficulty. Throughout the curriculum, learners are sequentially guided through all essential structure solution steps, from remote diffraction data collection and processing, various structure solution strategies, to structure quality evaluation. By the end of the curriculum, every student shares their experiences in an audio-supported slide-presentation over the web, while classmates provide feedback.We evaluate learners' confidence, academic performance and sustained skill level through a multi-facetted set of assessments, including quizzes, problem set solutions, final presentations, peer feedback, pre- and post course surveys, as well as a comprehensive structure solution set to be solved in a longterm post-test. Participants showed good problem solving- and very good crystallographic communication and feedback skills. Their confidence level applying x-ray crystallography techniques increased during the course. Most importantly, three months after the course, >80% of participants were able to solve and refine unknown diffraction datasets to publication quality level.