236 results on '"Pearce H"'
Search Results
202. Emergency department shift co-ordinator's report.
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Pearce H
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- 2009
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203. ChemInform Abstract: Synthesis of 4-Amino-8-(2,2-difluoro-2-deoxy-β-D-ribofuranosyl amino)pyrimido(5,4-d)pyrimidine (DFARPP). Stability and Cellular Cytotoxicity.
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MABRY, T. E., JONES, C. D., CHOU, T. S., COLACINO, J. M., GRINDEY, G. B., WORZALLA, J. F., and PEARCE, H. L.
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- 1995
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204. Pathological changes in the lunS of a horse which had a history of intermittent nasal bleeding after exercise
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Badcoe, L. M., Thompson, K. G., and Pearce, H. E.
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A 4-year-old thoroughbred-cross gelding was presented with a history of intermittent nasal bleeding after exercise for the past 2 years.
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- 1992
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205. Modern Drafting
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Pearce, H. O.
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- 1945
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206. HENNEPIN, III., June 17, 1858.
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PEARCE, H. C.
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- 1858
207. Vertical Versus Shared Leadership as Predictors of the Effectiveness of Change Management Teams: An Examination of Aversive, Directive, Transactional, Transformational, and Empowering Leader Behaviors.
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Pearce, Craig L. and Sims Jr., Henry P.
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- *
LEADERSHIP , *CHANGE management , *TEAMS in the workplace , *ORGANIZATIONAL effectiveness , *MANAGEMENT , *ORGANIZATIONAL sociology - Abstract
This study investigated vertical versus shared leadership as predictors of the effectiveness of 71 change management teams. Vertical leadership stems from an appointed or formal leader of a team, whereas shared leadership (C. U Pearce, 1997; C. L. Pearce & J. A. Conger, ha press; C. L. Pearce & H. P. Sims, 2000) is a group process in which leadership is distributed among, and stems from, team members. Team effectiveness was measured approximately 6 months after the assessment of leadership and was also measured from the viewpoints of managers, internal customers, and team members. Using multiple regression, the authors found both vertical and shared leadership to be significantly related to team effectiveness (p < .05), although shared leadership appears to be a more useful predictor of team effectiveness than vertical leadership. [ABSTRACT FROM AUTHOR]
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- 2002
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208. Designing Neural Networks for Real-Time Systems
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Pearce, Hammond, Yang, Xin, Roop, Partha S., Katzef, Marc, Strøm, Tórur Biskopstø, Pearce, Hammond, Yang, Xin, Roop, Partha S., Katzef, Marc, and Strøm, Tórur Biskopstø
- Abstract
Artificial Neural Networks (ANNs) are increasingly being used within safety-critical Cyber-Physical Systems (CPSs). It is important to validate both the timing and functional correctness of these systems. However, most approaches in the literature consider guaranteeing only the functionality of ANN based controllers. This issue stems largely from the implementation strategies used within common neural network frameworks — their underlying source code is often simply unsuitable for formal techniques such as static timing analysis. As a result, developers of safety-critical CPS must rely on informal techniques such as measurement based approaches to prove correctness, techniques that provide weak guarantees at best. In this work we address this challenge. We propose a design pipeline whereby neural networks trained using the popular deep learning framework Keras are compiled to functionally equivalent C code. This C code is restricted to simple constructs that may be analysed by existing static timing analysis tools. As a result, if compiled to a suitable time-predictable platform all execution bounds may be statically derived. To demonstrate the benefits of our approach we execute an ANN trained to drive an autonomous vehicle around a race track. We compile the ANN to the Patmos time-predictable controller, and show that we can derive worst case execution timings.
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- 2021
209. Faster function blocks for precision timed industrial automation
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Pearce, Hammond, Roop, Partha, Biglari-Abhari, Morteza, Schoeberl, Martin, Pearce, Hammond, Roop, Partha, Biglari-Abhari, Morteza, and Schoeberl, Martin
- Abstract
In industrial automation, safety-critical control systems need robust timing guarantees in addition to functional correctness. Unfortunately, devices that are typically used in this domain, such as Programmable Logic Controllers, often feature architectures that are not amenable to static timing analysis, for instance relying on general purpose microprocessors or embedded operating systems. As a result, designers often rely on timing values gained from simple measurement of running applications, an approach that only provides very weak guarantees at best. The synchronous approach for IEC 61499 Function Blocks, in contrast, has been demonstrated to be time predictable when run on appropriate hardware, such as simple microprocessors. However, simple microprocessors are often not fast or powerful enough for modern automation requirements. In this paper, we examine how the performance of synchronous IEC 61499 can be improved through the usage of the multi-core T-CREST architecture, data scratchpads, and an optimised compiler. Overall, our improvements resulted in 60% shorter worst-case execution times.
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- 2018
210. Students' Representative Council (60th), University of Melbourne, 1965-1966.
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Group photograph mounted on white cardboard on which are printed title and names of individuals. (Absent: P. Mulqueen, D. Muirden, T. Rees, I. Robinson, A. McGregor, G. Devries (P.R.O.)) Present: W. Pearce, H. Reese, R. Bond, R. Guy, K. Romuld, H. Schroor, R. Hinkley, Miss J. Harry, A.Christie, A. Marsland, A. Finn, R. Menzies, B. McGowen, Miss D. Nash, D. Morawetz, A. Williams, G. Simon, Miss B. Peter, N. Ackman, Mrs. Nancy Hosie, R. Kemp (Treas.), Previous Control Number: UMA/I/1689 Previous Control Number: OSBA/483, 201673 Item: [2017.0071.00747] "Students' Representative Council (60th), University of Melbourne, 1965-1966."
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- 1965
211. A Collection of songs and madrigals by English composers of the close of the fifteenth century. Prepared for the members of the Plainsong and Mediaeval Music Society.
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Pearce, Charles William, Pearce, Charles William, 1856-1928., Plainsong and Mediaeval Music Society (Great Britain), Pearce, Charles William, Pearce, Charles William, 1856-1928., and Plainsong and Mediaeval Music Society (Great Britain)
- Abstract
3 p. l., xviii p., 2 l., 10 p., 1 l., 31 p. 38 cm., Half-title and binder's title: Songs and madrigals of the fifteenth century., Accompanied by 8 sheets of music (ancient notation) in portfolio., "300 copies printed, of which this is no. 151.", "The accompaniments to the songs have been written specially for this work by Dr. C. W. Pierce.", Sections of introduction are variously signed "C. W. P." (i.e. C. W. Pearce) "H. B. B." (i.e. H. B. Briggs) and "A. H.-H." (i.e. A. Hughes-Hughes), Copy 2: Preservation photocopy includes the accompanying 8 sheets of music in 7 volumes in pocket inside back cover., (dlps) ADY9226.0001.001, (lccallno) M 2 .C7, http://quod.lib.umich.edu/t/text/accesspolicy.html
- Published
- 1891
212. Various models for company files, Southern California, 1936
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Dick Whittington Studio and Dick Whittington Studio
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- 1936, United States., California., California, Southern., Californie., Californie (Sud), California., Southern California., United States.
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13 photographs of various women's modeling shots, Southern California, 1936. "Subject: Girls, Various models for our files, #1- Marjorie Stanley, #2- Dorothy Sanders, #3 Geraine Greer, #4 Marjorie Timm, #5 Evelyn Steele, #6 Dara Seberger, #7 Nancy Page, #8 Miss McDonald, #9 Harley Wood, #10 Maxine Sturges, #11 Miss Clow, #12 Jean Bunt, #13 Nancy Did[ilg], #14 Adele Pearce, H. women models; Client: Whittington; Job: 62-21-157; Year: 1936; Original print order: 1 ea[ch]; Size: 5x7, contact; Finish: gl[ossy]; Mount: D.W.; Re-order: 1 ea[ch] #3, 8; Size: 8x10; Finish: dull; Mount: B" -- on envelope front. "Photographer: Howar[d]" -- on envelope back.
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- 1936
213. Students' Representative Council (60th), University of Melbourne, 1965-1966.
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University Of Melbourne Archives and University Of Melbourne Archives
- Abstract
Group photograph mounted on white cardboard on which are printed title and names of individuals. (Absent: P. Mulqueen, D. Muirden, T. Rees, I. Robinson, A. McGregor, G. Devries (P.R.O.)) Present: W. Pearce, H. Reese, R. Bond, R. Guy, K. Romuld, H. Schroor, R. Hinkley, Miss J. Harry, A.Christie, A. Marsland, A. Finn, R. Menzies, B. McGowen, Miss D. Nash, D. Morawetz, A. Williams, G. Simon, Miss B. Peter, N. Ackman, Mrs. Nancy Hosie, R. Kemp (Treas.), 201673 Item: [2017.0071.00747] "Students' Representative Council (60th), University of Melbourne, 1965-1966."
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- 1965
214. A Collection of songs and madrigals by English composers of the close of the fifteenth century. Prepared for the members of the Plainsong and Mediaeval Music Society.
- Author
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Pearce, Charles William, 1856-1928., Pearce, Charles William, 1856-1928., Plainsong and Mediaeval Music Society (Great Britain), Pearce, Charles William, 1856-1928., Pearce, Charles William, 1856-1928., and Plainsong and Mediaeval Music Society (Great Britain)
- Abstract
3 p. l., xviii p., 2 l., 10 p., 1 l., 31 p. 38 cm., Half-title and binder's title: Songs and madrigals of the fifteenth century., Accompanied by 8 sheets of music (ancient notation) in portfolio., "300 copies printed, of which this is no. 151.", "The accompaniments to the songs have been written specially for this work by Dr. C. W. Pierce.", Sections of introduction are variously signed "C. W. P." (i.e. C. W. Pearce) "H. B. B." (i.e. H. B. Briggs) and "A. H.-H." (i.e. A. Hughes-Hughes), Copy 2: Preservation photocopy includes the accompanying 8 sheets of music in 7 volumes in pocket inside back cover., (dlps) ADY9226.0001.001, (lccallno) M 2 .C7, http://quod.lib.umich.edu/t/text/accesspolicy.html
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- 1891
215. name game.
- Abstract
BOX 1: In 2018, Adele (pictured) officiated at the wedding of which comedian? Robert Webb (G) Russell Brand (M) Alan Carr (K) BOX 2: Foolish Loving Spaces is the latest album from which British indie band? Blossoms (E) Buds (C) Blooms (Y) BOX 3: Who won this year's Oscar for Best Actress? Saoirse Ronan (S) Renee Zellweger (L) Charlize Theron (I) BOX 4: Who wrote children's book Charlotte's Web in 1952? Philippa Pearce (H) Mary Norton (W) E. B. White (A) BOX 5: How old was footballer Pele when he made his debut for Brazil? Sixteen (I) Seventeen (F) Eighteen (T) BOX 6: In what year did Ceylon become Sri Lanka? 1952 (G) 1962 (T) 1972 (M) BOX 7: Who wrote the novel upon which the Netflix series The Stranger is based? Harlan Coben (A) Lee Child (L) Michael Connelly (S) BOX 8: Bruce Forsyth had a role in which children's film? Chitty Chitty Bang Bang (D) Bedknobs And Broomsticks (U) Annie (X) BOX 9: Micheline Roquebrune is the wife of which actor? David Jason (N) Richard Gere (P) Sean Connery (E) BOX 10: Where is the El Yunque National Forest? Puerto Rico (T) Ecuador (H) Brazil (I) Yesterday's answer: ADAM HILLS (1A, 2L, 3S, 4L, 5H, 6I, 7M, 8D, 9A) [ABSTRACT FROM PUBLISHER]
- Published
- 2020
216. name game.
- Abstract
BOX 1: What was the name of the character Lynda Bellingham (right) played in sitcom Second Thoughts? Faye Bagshott (M) Faith Greyshott (T) Fern Walcott (L) BOX 2: Who is the director of new epic war film 1917? Roland Emmerich (N) Steven Spielberg (W) Sam Mendes (A) BOX 3: How many Olympic gold medals has Jamaican former sprinter Usain Bolt won? Four (E) Six (Y) Eight (R) BOX 4: Which Welsh actor had a daughter with Swedish actress Anna Lundberg in September? Michael Sheen (N) Ioan Gruffudd (L) Rhys Ifans (U) BOX 5: Which former Neighbours star played Scrooge in a recent BBC adaptation of A Christmas Carol? Alan Dale (N) Guy Pearce (H) Jason Donovan (C) BOX 6: Cathryn Wigglesworth is the real name of which Coronation Street actress? Beverley Callard (T) Kate Ford (F) Helen Worth (O) BOX 7: Which science fiction author wrote the trilogy known as The Fionavar Tapestry? Iain Banks (T) Guy Gavriel Key (Y) Philip K. Dick (A) BOX 8: What is a polemic? A divisive work of fiction (K) A strong attack or defence of a particular opinion (T) A political strategist (U) Thursday's answer: EVA GREEN (1E, 2R, 3E, 4G, 5A, 6V, 7N, 8E). [ABSTRACT FROM PUBLISHER]
- Published
- 2020
217. Prefrontal cortex and hippocampus in behavioural flexibility and posttraumatic functional recovery:Reversal learning and set-shifting in rats
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Rytter, Hana Malá, Andersen, Lykke Grønbech, Christensen, Rie Friis, Felbinger, Anita, Hagstrøm, Julie, Meder, David, Pearce, Hadley, Mogensen, Jesper, Rytter, Hana Malá, Andersen, Lykke Grønbech, Christensen, Rie Friis, Felbinger, Anita, Hagstrøm, Julie, Meder, David, Pearce, Hadley, and Mogensen, Jesper
- Published
- 2015
218. Cognitive flexibility and functional recovery regarding intradimensional and extradimensional shifts after prefrontal and/or hippocampal brain injury
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Rytter, Hana Malá, Andersen, Lykke Grønbech, Christensen, Rie Friis, Felbinger, Anita, Hagstrøm, Julie, Meder, David, Pearce, Hadley, Mogensen, Jesper, Rytter, Hana Malá, Andersen, Lykke Grønbech, Christensen, Rie Friis, Felbinger, Anita, Hagstrøm, Julie, Meder, David, Pearce, Hadley, and Mogensen, Jesper
- Published
- 2014
219. Rhinusa linariae
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Caldara, Roberto and To��evski, Ivo
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Coleoptera ,Curculionidae ,Insecta ,Arthropoda ,Animalia ,Rhinusa linariae ,Biodiversity ,Rhinusa ,Taxonomy - Abstract
Rhinusa linariae (Panzer, 1795) Figs 1, 10, 11, 24, 27, 33, 39 Curculio linariae Panzer, 1795: 18. Caldara 2008: 126 (nomen protectum). Cionus linariae (Panzer). Germar 1821: 314. Miarus linariae (Panzer). Stephens 1831: 16. Gymnetron linariae (Panzer). Rosenschoeld 1838: 769. H. Brisout de Barneville 1863: 651. Desbrochers des Loges 1893: 35. Reitter 1907: 43; 1916: 231. Hustache 1932: 412, 429. Hoffmann 1958: 1281, 1311. Smreczyński 1976: 35. Lohse & Tischler 1983: 267. Mecinus linariae (Panzer). Bedel 1885: 146; 1887: 307. Sainte-Claire Deville 1924: 69. Rhinusa linariae (Panzer). Caldara 2001: 185; 2008: 126. Caldara et al. 2010: 52. Alonso-Zarazaga et al. 2017: 203. Curculio curvirostris Rossi, 1790: 117. Hellwig 1795: 125. H. Brisout de Barneville 1863: 652. Caldara 2008: 126 (nomen oblitum). Type locality. Eberswalde (Brandeburg, Germany). Type series. The neotype was designated by Caldara (2008). Synonyms. Curculio curvirostris was described by Rossi (1790) from specimens collected in Tuscany (Italy). The neotype of this species (type locality: Italy, Lazio, Roma, Formello) was recently designated and its synonymy with R. linariae discussed by Caldara (2008). The junior name linariae can be maintained, since Curculio curvirostris Rossi is a junior homonym of Curculio curvirostris Fabricius, 1781, an Indonesian baridine, and also of Curculio curvirostris Herbst, 1784, a probable synonym of Exapion fuscirostre fuscirostre (Fabricius, 1775) (Alonso- Zarazaga et al. 2017). Redescription. Male. Body: oval, stout (Fig. 1). Rostrum: black, short (Rl/Pl 0.75); in lateral view strongly curved, very stout in basal third, further slightly narrowed to apex (Fig. 10); in dorsal view with subparallel sides, with moderately visible scrobes, striate-punctate in basal two thirds, smoother and shining along midline and in apical third, with sparse recumbent short (l/w 2���3) whitish scales in basal third. Head: between eyes as wide as rostrum at base, without fovea. Eyes almost flat. Antennae: dark brown, inserted at middle of rostrum; scape 2.5�� longer than wide, funicle distinctly longer than scape, with segment 1 twice longer than wide, slightly stouter than and about as long as segment 2, which is 2.5�� longer than wide, segments 3���5 gradually becoming more transverse; club oval, with segment 1 pubescent similarly to others. Pronotum: black, densely and rather regularly punctate, intervals between punctures narrow, smooth and shining, well visible between sparse recumbent moderately long (l/ w 4���6) subelliptical whitish scales; transverse (Pw/Pl 1.35), with distinctly rounded sides, widest between basal and middle thirds, distinctly constricted at apex, almost flat. Elytra: black, 1.28�� as long as wide, subelliptical, 1.32�� as wide as pronotum, with basal margin transverse until interstria 5, further beveled anteriorly, with moderately rounded sides, widest at middle, weakly convex on disc; interstriae well visible between sparse recumbent, elliptical to sublanceolate whitish scales, 0.50���0.75�� as long as width of interstria (l/w 4���7) and arranged in 2���3 irregular rows; striae well visible, half as wide as interstriae, with a row of scales slightly narrower than those on interstriae. Legs: stout, with sparse recumbent to suberect whitish scales distinctly shorter than width of tibia; femora black, subclavate, without tooth; tibiae black, stout, distinctly sinuous at middle, with outer margin moderately carinate; unci black, thin, all of same size; tarsi brown, with tarsomere 1 1.7�� longer than wide, tarsomere 2 1.2�� longer than wide, tarsomere 3 bilobed and distinctly wider than tarsomere 2, onychium as long as tarsomeres 1���3 taken together; claws dark brown, equal in length. Venter: metasternum black, with sparse subrecumbent long setiform whitish scales. Mesepimera, mes- and metepisterna with sparse long setiform whitish scales. Abdomen black, with dense and somewhat regular punctures, well visible between sparse recumbent to subrecumbent setiform whitish scales; length ventrites 1+2/3+4 1.95 Penis (Fig. 27): body of penis very long (l/ w 6.0), nearly parallel-sided, with broad apex; apodemes distinctly shorter than body, in lateral view forming an angle of 60 degrees in relation to body. Female. Rostrum slightly longer (Rl/Pl 0.81) (Fig. 11), in dorsal view smooth and shining from antennal insertion to apex, antennae inserted between basal and middle thirds of rostrum. Spiculum ventrale: arms moderately spaced each other, apodeme slightly longer than arms (Fig. 33). Spermatheca: ramus very short, collum well-developed (Fig. 39). Variability. Length 2.6���3.1 mm. Pronotum and elytra vary slightly both in curvature of sides and convexity. The rostrum varies a little in length in both sexes (Rl/Pl male 0.72���0.78; female 0.78���0.85). Remarks and comparative notes. The three species of this group are very similar to each other and have very similar shape of the genitalia. Rhinusa linariae differs from the other two species by the completely black integument. In addition, it differs from R. brisouti by the less broad pronotum, the more elongate elytra and the protibiae being less distinctly carinate and with the outer margin less directed outward apically, and from R. kumatschevi by the protibiae being more deeply sinuate in the middle. Biological notes. Rhinusa linariae is a univoltine root galling weevil. Gall induction and larval development are mainly recorded on Linaria vulgaris Mill. and rarely on L. genistifolia (L.) P. Mill. in Southeastern Europe. Adults emerge in early spring, feed and copulate on the top of the young toadflax shoots. Females lay eggs singly or in small groups on toadflax roots or below root crowns. Shortly after, oviposition triggers gall formation, and larvae feed on galled root tissue through three instars. Pupation occurs in galls, while new adults emerge in mid- to late summer or rarely stay inside galls during winter, overwintering in soil or in plant litter close to their host plant. Twenty-five years ago, Rhinusa linariae was introduced as a biological control agent for invasive toadflaxes in Canada and USA, where it was recently confirmed as established only in British Columbia; however, the current populations are still too low to have a significant biological impact (Sing et al. 2015). Distribution. This species is widely distributed in the whole Europe, Turkey, Kazakhstan and Western Siberia. Introduced in North America. Non-type material examined. France: Paris (3, BMNH). Italy: Piemonte, Asti, Nizza Monferrato, Frazione Barca, 28.VII.1978, leg. Diotti (1, LDCC); Lombardia, Monza Parco, IV.1948, leg. Loro (1, MSNM); Lombardia, Lecco, Monte Barro, 15.VI.1990, leg. Leonardi (14, MSNM); Veneto, Verona, Fumane, 17.V.1981, leg. Osella (1, GOCV); Veneto, Vicenza, Alta Val d'Astico, 21.VI.1997, leg. Diotti (1, LDCC); Veneto, Vicenza, Monti Berici, Lago di Fimon, 26.IV.2010, leg. Diotti (1, LDCC); Veneto, Vicenza, Monti Berici, Soghe, 19.IV.2011, leg. Diotti (1, LDCC); Friuli-Venezia Giulia, Gorizia, Grado, Belvedere, 12.VI.1912, leg. Springer (1, MSNM); Friuli-Venezia Giulia, Trieste, Prosecco, 10.V.1925, leg. Springer (1, MSNM); Friuli-Venezia Giulia, Trieste, Zaule, 10.V.1923, leg. Springer (1, MSNM); Liguria, Genova, Madonna della Guardia, 4.VII.2002, leg. Diotti (1, LDCC); Liguria, Genova, Gattorna, 1���10.VII.1979, leg. Caldara (3, RCCM); Liguria, La Spezia, 6.VI.1971, leg. Caldara (1, RCCM); Liguria, La Spezia, Castelnuovo Magra, 12.V.1980, leg. Caldara (1, RCCM); Liguria, Savona, Celle Ligure, 10.V.1969, leg. Ragozzino (1, RCCM); Emilia Romagna, Piacenza, Bettola, 28.V.1970, leg. Caldara (1, RCCM); Toscana, Firenze, V.1942, leg. Lombardi (2, MSNM); Toscana, Firenze, Palazzuolo, 14.V.2000, leg. Mingazzini (1, LDCC); Toscana, Livorno, Piombino, 8.V.1971, leg. Caldara (1, RCCM); Lazio, Roma, Palestrina, Frazione Carchitti, 20.V.1973 (1, RCCM); Lazio, Roma, Rocca di Papa, Monte Cavo, 900 m, 30.V.2005, leg. Colonnelli (1, ECCR); Abruzzo, L'Aquila, Raiano, 10.V.2002, leg. Osella (1, GOCV); Abruzzo, Pescara, Popoli, 19.V.1996, leg. Osella (2, GOCV); Abruzzo, Pescara, Popoli, Sorgenti fiume Pescara, 11.V.2002, leg. Osella (3, GOCV); Abruzzo, Teramo, Campo Battaglia, 5.V.2002, leg. Osella (2, GOCV); Italy (Sicily): Messina, Monti Nebrodi, Alcara Li Fusi, 27.IV.2016, leg. Casalini (RCCR); Messina, Monte Etna, Piano Provenzana, 1800-2200 m, on Linaria purpurea, 22.VI.2012, leg. Baviera (CBCM). Germany: Baden-W��rttemberg, Leimen near Heidelberg, 400 m, 27.V.1990, leg. Ko��ť��l (1, MKCH). Hungary: Mecsek Mts., Hossz��het��ny Zeng�� Hill, 400 m, 25.IV.1987, leg. Ko��ť��l (1, MKCH). Slovenia: Istria, Podgorje, 28.X.1937, leg. Springer (1, MSNM); Nanos Mount, 750 m, 18.V.1994, leg. Ko��ť��l (1, MKCH). Romania: B��zi��s, leg. Zoppa (1, MSNM); C��mpia Crişurilor, Rovine near Arad, 21.IV.1983, leg. Ko��ť��l (1, MKCH). Turkey: Kirşehir, 4 km S of Mucur, 10.V.1997, leg. Sama (1, FTCM). Russia: Volgogradskaya Oblast���, 8 km E of Perekopskij, VI.2005, leg. Tronci (1, ECCR). Kazakhstan: Aktjubinsk, Karaholda River, Novoalekseevka, 26.V.2000 (1, RCCM)., Published as part of Caldara, Roberto & To��evski, Ivo, 2019, Rhinusa Stephens: a taxonomic revision of the species belonging to the R. linariae R. herbarum, R. melas, and R. mauritii groups (Coleoptera Curculionidae), pp. 318-340 in Zootaxa 4679 (2) on pages 320-322, DOI: 10.11646/zootaxa.4679.2.6, http://zenodo.org/record/3772583, {"references":["Panzer G. W. F. (1795) Deutschlands Insectenfaune oder entomologisches Taschenbuch fur das Iahr 1795. Entomologica Germanica exhibens insecta per Germaniam indigena secundum classes, ordines, genre, species adiectis synonymis, locis, observationibus. I. Eleuterata. Cum Tabulis Aeneis. Apud Felseckeri haeredes, Norimbergae, [8 unn. pp, Vorbericht] + 12 pl. + [24 unn. pp.] + 372 pp. https: // doi. org / 10.5962 / bhl. title. 11756","Germar, E. F. (1821) Genera quaedam Curculionitum proposita, et speciebus observatis illustrata. Magazin der Entomologie, 4, 291 - 345.","Stephens, J. F. (1831) Illustrations of British entomology; or, a synopsis of indigenous insects: containing their generic and specific distinctions; with an account of their metamorphoses, times of appearance, localities, food, and economy, as far as practicable. Mandibulata. Vol. IV. Signature C (pp. 15 - 30, pl. XX). Baldwin, Cradock, London, 413 + [1] pp., pls. XX- XXIII.","Brisout de Barneville, H. (1863) Monographie du genre Gymnetron. [Cont.]. Annales de la Societe Entomologique de France, Series 4, 2 (4), 637 - 668. [1862] https: // doi. org / 10.1080 / 00379271.1863.11755420","Desbrochers des Loges, J. (1893) Revision des especes de curculionides appartenant a la tribu des Gymnetridae d'Europe et circa. Le Frelon, 3, 1 - 68.","Reitter, E. (1907) Bestimmung-Tabellen der europaischen Coleopteren. LIX Heft. Curculionidae. 13 Theil: Mecinini (Gymnetrini). Verhandlungen des naturforschenden Vereines in Brunn, 1907, 1 - 50.","Reitter, E. (1916) Fauna Germanica. Die Kafer des Deutsches Reiches. Nach der analytische Methode bearbeitet. V. Band. K. G. Lutz, Stuttgart, 343 pp., pls. 153 - 168.","Hustache, A. (1932) Curculionidae gallo-rhenans. Annales de la Societe Entomologique de France, 100, 153 - 470. [1931]","Hoffmann, A. (1958) Coleopteres Curculionides. Troisieme partie. Faune de France, 62, 1209 - 1839.","Smreczynski, S. (1976) Klucze do oznaczania owadow polski. XIX (98 f). Coleoptera, Curculionidae, Curculioninae. Polski Towarzystwo Entomologiczene, Wroclaw, 115 pp.","Lohse, G. A. & Tischler, T. (1983) Mecininae. In: Freude, H., Harde, K. W. & Lohse, G. A. (Eds.), Die Kafer Mitteleuropas. Band 11. Goecke & Evers, Krefeld, pp. 259 - 283.","Bedel, L. (1885) Faunes des Coleopteres du Bassin de la Seine. Vol. VI. Rhynchophora (Cont.). Annales de la Societe entomologique de France, Hors Serie, Series 6, 5, 145 - 200.","Bedel, L. (1887) Faunes des Coleopteres du Bassin de la Seine. Vol. VI. Rhynchophora (Cont.). Annales de la Societe entomologique de France, Hors Serie, Series 6, 7 (3), 361 - 384.","Sainte-Claire Deville, J. (1924) Faune d. es Coleopteres du Bassin de la Seine par Louis Bedel. Tome VI bis (Supplement aux Rhynchophora) redige d'apres les notes de L. Bedel. Publications Hors Serie. Societe Entomologique de France, Paris, 159 pp.","Caldara, R. (2001) Phylogenetic analysis and higher classification of the tribe Mecinini (Coleoptera: Curculionidae, Curculioninae). Koleopterologische Rundschau, 71, 171 - 203.","Caldara, R., Sassi, D. & Tosevski, I. (2010) Phylogeny of the weevil genus Rhinusa Stephens based on adult morphological characters and host plant information (Coleoptera: Curculionidae). Zootaxa, 2627 (1), 39 - 56. https: // doi. org / 10.11646 / zootaxa. 2627.1.3","Alonso-Zarazaga, M. A., Barrios, H., Borovec, R., Bouchard, P., Caldara, R., Colonnelli, E., Gultekin, L., Hlavac, P., Korotyaev, B., Lyal, C. H. C., Machado, A., Meregalli, M., Pierotti, H., Ren, L., Sanchez-Ruiz, M., Sforzi, A., Silfverberg, H., Skuhrovec, J., Tryzna, M., Velazquez de Castro, A. J. & Yunakov, N. N. (2017) Cooperative Catalogue of Palaearctic Coleoptera Curculionoidea. Monografias electronicas de la Sociedad Entomologica Aragonesa, 8, 1 - 729.","Rossi, P. (1790) Fauna etrusca sistens insecta quae in provinciis florentina et pisana praesertim collegit Petrus Rossius in Regio Pisano Athenaeo. Tomus primus. Typis Thomae Masi, Sociorum, Liburni, xxii + 272 pp, 1 pl. https: // doi. org / 10.5962 / bhl. title. 15771","Hellwig, J. C. L. (1795) [new taxa]. In: Rossi, P. Fauna Etrusca sistens insecta quae in provinciis Florentina et Pisana praesertim collegit Petrus Rossius in regio pisano athenaeo. Tomus primus cum XI Tab. Mantissae priore parte adiecta iterum edita et annotatis perpetuis aucta a D. Ioh. Christ. Lud. Hellwig. C. G. Fleckeisen, Helmstadii, pp. i-xxviii + 1 - 457 pp., 12 pls.","Sing, S. E., De Clerck-Floate, R., Hansen, R. W., Pearce, H., Randall, C. B., Tosevski, I. & Ward, S. M. (2015) Biology and biological control of Dalmatian and Yellow Toadflax. USDA Forest Service, Forest Health Technology Enterprise Team, Morgantown, West Virginia, 154 pp. [FHTET- 2015 - 03] https: // doi. org / 10.5962 / bhl. title. 149716"]}
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- 2019
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220. Association analysis of phenotypic traits in selected and propagated lines derived from different winter wheat CCPs
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Dennenmoser, Dominic, Schmidt, Jan Henrik, and Backes, Gunter
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Winter wheat ,GAPIT-related GWAS ,Composite cross population (CCP) ,Network-based GWAS ,Triticum aestivum ,Self-regulating systems ,SNP ,Biodiversity ,Genome-wide association studies (GWAS) ,Plant breeding - Abstract
Background Biodiversity at all levels from microbial populations up to inter- and intra-specific diversity of plants is a major component in self-regulating systems. Aims and methods for breeding of crops adapted to self-regulating systems, such as heterogeneous populations, are different from most current breeding programs in several aspects. This study attempts to use wheat lines selected from composite cross populations (CCPs) in order to test the applicability as diverse, but still restricted population for genome-wide association studies (GWAS). Material and Methods Out of 200 CCP lines, derived from different winter wheat CCPs, DNA was isolated from 184, selected for contrasting phenotypes and homozygosity and genotyped using a 20K (15K+5K) SNP micro array chip. The genotyping data, together with the phenotypic data are being used for the GWAS to link allelic changes to trait expressions. GAPIT-related GWAS: GLM, MLM, MLMM, and FarmCPU included K and Q matrix. Covariate (Q matrix) was calculated using principal component analysis (PCA) based on 8961 selected SNPs. Network-based GWAS: using Gaussian copula graphical model for reconstructing conditional independence networks. Results Preliminary GWAS results were obtained from up to ten phenotypic traits at the organically and conventional managed trial farms of the University of Kassel (KU) and the Technical University of Munich (TUM; Germany) for the season 2017/18. GAPIT-related GWAS resulted in up to 13 candidate for up 6 of the 10 traits and 31--65 potential candidates for all traits. In contrast, the network-based GWAS resulted in 50--52 candidates for 9 of the 10 traits. Conclusions Further investigations and comparison with different environments and methods are needed for a more complex understanding of the phenotypic traits in the development of diverse populations and mixtures in organic plant breeding., {"references":["Behrouzi, P., Arends, D., & Wit, E. C. (2018). Netgwas: An R package for network-based genome-wide association studies. arXiv:1710.01236v4 [stat.AP].","Behrouzi, P., & Wit, E. C. (2019). Detecting epistatic selection with partially observed genotype data by using copula graphical models. Journal of the Royal Statistical Society: Series C (Applied Statistics), 68(1):141–160.","Döring, T. F., Annicchiarico, P., Clarke, S., Haigh, Z., Jones, H. E., Pearce, H., Snape, J., Zhan, J., & Wolfe, M. S. (2015). Comparative analysis of performance and stability among composite cross populations, variety mixtures and pure lines of winter wheat in organic and conventional cropping systems. Field Crops Research, 183:235–245.","Döring, T. F., Pautasso, M., Finckh, M. R., & Wolfe, M. S. (2011). Concepts of plant health—reviewing and challenging the foundations of plant protection. Plant Pathology, 61(1):1–15.","Lipka, A. E., Tian, F., Wang, Q., Peiffer, J., Li, M., Bradbury, P. J., Gore, M. A., Buckler, E. S., & Zhang, Z. (2012). Gapit: Genome association and prediction integrated tool. Bioinformatics, 28(18):2397–2399.","Loreau, M. (2010). Linking biodiversity and ecosystems: Towards a unifying ecological theory. Philosophical transactions of the Royal Society of London. Series B, Biological sciences, 365(1537):49–60.","Tang, Y., Liu, X., Wang, J., Li, M., Wang, Q., Tian, F., Su, Z., Pan, Y., Liu, D., Lipka, A. E., Buckler, E. S., & Zhang, Z. (2016). Gapit version 2: An enhanced integrated tool for genomic association and prediction. The Plant Genome Journal, 9(2):1–9.","Wang, J., & Zhang, Z. (2019). GAPIT version 3: An interactive analytical tool for genomic association and prediction. Retrieved February 19, 2019, from https://github.com/jiabowang/GAPIT3."]}
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- 2019
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221. Delayed intensive acquisition training alleviates the lesion-induced place learning deficits after fimbria-fornix transection in the rat
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Rytter, Hana Malá, Rodriguez Castro, Maria, Pearce, Hadley Leighton, Kingod, Siff Camilla, Nedergaard, Signe Kjær, Scharff, Zakaryiah, Zandersen, Maja, Mogensen, Jesper, Rytter, Hana Malá, Rodriguez Castro, Maria, Pearce, Hadley Leighton, Kingod, Siff Camilla, Nedergaard, Signe Kjær, Scharff, Zakaryiah, Zandersen, Maja, and Mogensen, Jesper
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- 2012
222. Comparison of changes in bone density and turnover with abacavir-lamivudine versus tenofovir-emtricitabine in HIV-infected adults: 48-week results from the ASSERT study
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Stellbrink, Hans-Jürgen, Orkin, Chloe, Arribas, Jose Ramon, Compston, Juliet, Gerstoft, Jan, Van Wijngaerden, Eric, Lazzarin, Adriano, Rizzardini, Giuliano, Sprenger, Herman G, Lambert, John D. C., Sture, Gunta, Leather, David, Hughes, Sara, Zucchi, Patrizia, Pearce, Helen, Stellbrink, Hans-Jürgen, Orkin, Chloe, Arribas, Jose Ramon, Compston, Juliet, Gerstoft, Jan, Van Wijngaerden, Eric, Lazzarin, Adriano, Rizzardini, Giuliano, Sprenger, Herman G, Lambert, John D. C., Sture, Gunta, Leather, David, Hughes, Sara, Zucchi, Patrizia, and Pearce, Helen
- Abstract
Abacavir-lamivudine and tenofovir DF-emtricitabine fixed-dose combinations are commonly used as first-line antiretroviral therapies. However, few studies have comprehensively compared their relative safety profiles.
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- 2010
223. Vertical versus shared leadership as predictors of the effectiveness of change management teams: An examination of aversive, directive, transactional, transformational, and empowering leader behaviors
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Craig L. Pearce and Henry P. Sims
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Team composition ,Social Psychology ,media_common.quotation_subject ,Team effectiveness ,Psychological safety ,Shared leadership ,Leadership ,Situational leadership theory ,Transactional leadership ,Leadership style ,Psychology ,Social psychology ,Applied Psychology ,media_common - Abstract
This study investigated vertical versus shared leadership as predictors of the effectiveness of 71 change management teams. Vertical leadership stems from an appointed or formal leader of a team, whereas shared leadership (C. L. Pearce, 1997; C. L. Pearce & J. A. Conger, in press; C. L. Pearce & H. P. Sims, 2000) is a group process in which leadership is distributed among, and stems from, team members. Team effectiveness was measured approximately 6 months after the assessment of leadership and was also measured from the viewpoints of managers, internal customers, and team members. Using multiple regression, the authors found both vertical and shared leadership to be significantly related to team effectiveness ( p .05), although shared leadership appears to be a more useful predictor of team effectiveness than vertical leadership. The increasing use of empowered teams and concomitant flattening of organizational structures (Mohrman, Cohen, & Mohrman, 1995) brings into question the more traditional models of leadership. What kind of leadership is more appropriate for this new team-based environment? Pearce (1997), Yukl (1998), Pearce and Sims (2000), and Pearce and Conger (in press) have suggested that shared leadership—leadership that emanates from the members of teams, and not simply from the appointed team leader— may provide the answer to this question. Thus, we investigated this issue within the context of change management teams (CMTs). The CMTs in this study are teams that, while not fully self-managing, have a very high degree of decision-making latitude for improving the operations in their respective areas of responsibility. Thus, our results are most applicable to highautonomy teams that engage in complex tasks, and they may not generalize to traditional work groups. The teams in this study are also all drawn from one organization, which helps to control for situational variables that might influence team effectiveness (e.g., organizational culture) but may limit the generalizability to alternate organizational contexts. More specifically, we explored how the behavior of the appointed team leaders (vertical leadership) versus distributed influence from within the team (shared leadership) accounted for the effectiveness of the CMTs. In this article, we briefly review the behavioral model of leadership that forms the basis of this study and then review literature related to the shared leadership process. We offer several hypotheses regarding the potential role of team leadership in team effectiveness. Subsequently, we describe our methods, present our results, and offer a discussion and conclusion.
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- 2002
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224. A genetic association study of maternal and fetal candidate genes that predispose to preterm prelabor rupture of membranes (PROM)
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Genetic research -- Physiological aspects ,Genomics -- Physiological aspects ,Public health -- Physiological aspects ,DNA -- Physiological aspects ,Health - Abstract
To link to full-text access for this article, visit this link: http://dx.doi.org/10.1016/j.ajog.2010.05.026 Byline: Roberto Romero (a)(b)(c), Lara A. Friel (a)(c), Digna R. Velez Edwards (e), Juan Pedro Kusanovic (a)(c), Sonia S. Hassan (a)(c), Shali Mazaki-Tovi (a)(c), Edi Vaisbuch (a)(c), Chong Jai Kim (a)(d), Offer Erez (a)(c), Tinnakorn Chaiworapongsa (a)(c), Brad D. Pearce (h), Jacquelaine Bartlett (f), Benjamin A. Salisbury (i), Madan Kumar Anant (i), Gerald F. Vovis (j), Min Seob Lee (k), Ricardo Gomez (l), Ernesto Behnke (l), Enrique Oyarzun (m), Gerard Tromp (b), Scott M. Williams (f)(g), Ramkumar Menon (h) Keywords: chorioamnionitis; DNA variants; extracellular matrix; genetic association study; genomics; genotype; haplotype; high dimensional biology; matrix metalloproteinase; parturition; prematurity; preterm prelabor rupture of membranes; single-nucleotide polymorphism Abstract: We sought to determine whether maternal/fetal single-nucleotide polymorphisms (SNPs) in candidate genes are associated with preterm prelabor rupture of membranes (pPROM). Author Affiliation: (a) Perinatology Research Branch, Eunice Kennedy Shriver National Institute of Child Health and Human Development/National Institutes of Health/Department of Health and Human Services, Bethesda, MD, and Detroit, MI (b) Center for Molecular Medicine and Genetics, Wayne State University, Detroit, MI (c) Department of Obstetrics and Gynecology, Wayne State University, Detroit, MI (d) Department of Pathology, Wayne State University, Detroit, MI (e) Vanderbilt Epidemiology Center, Institute of Medicine and Public Health, and the Department of Obstetrics and Gynecology, Vanderbilt University, Nashville, TN (f) Center for Human Genetics Research, Vanderbilt University, Nashville, TN (g) Department of Molecular Physiology and Biophysics, Vanderbilt University, Nashville, TN (h) Department of Epidemiology, Rollins School of Public Health, Emory University, Atlanta, GA (i) PGxHealth LLC, New Haven, CT (j) Helix Therapeutics LLC, New Haven, CT (k) Genaissance Pharmaceuticals Inc, New Haven, CT (l) Department of Obstetrics and Gynecology, Center for Perinatal Diagnosis and Research, Sotero del Rio Hospital, Santiago, Chile (m) Department of Obstetrics and Gynecology, Pontificia Universidad Catolica de Chile, Santiago, Chile Article History: Received 8 March 2010; Revised 10 April 2010; Accepted 18 May 2010 Article Note: (footnote) This research was supported, in part, by the Perinatology Research Branch, Division of Intramural Research, Eunice Kennedy Shriver National Institute of Child Health and Human Development, National Institutes of Health, Department of Health and Human Services., Drs Anant, Lee, Salisbury, and Vovis were formerly of Genaissance Pharmaceuticals Inc, New Haven, CT, where contributions to this work were initiated., Cite this article as: Romero R, Friel LA, Velez Edwards DR, et al. A genetic association study of maternal and fetal candidate genes that predispose to preterm prelabor rupture of membranes (PROM). Am J Obstet Gynecol 2010;203:361.e1-30.
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- 2010
225. Parasites Make It Hard to Fight Viruses
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Kristen L. Mueller
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Multidisciplinary ,Cell Biology ,Biology ,medicine.disease ,Biochemistry ,Virology ,Virus ,Antiviral immunity ,Immune system ,Immunology ,Coinfection ,medicine ,Helminths ,Molecular Biology - Abstract
Microbial coinfections challenge the immune system—different pathogens often require different flavors of immune responses for their elimination or containment (see the Perspective by Maizels and Gause). Two teams studied what happens when parasitic worms and viruses infect mice at the same time. Reese et al. found that parasite coinfection woke up a dormant virus. Osborne et al. found that mice already infected with parasitic worms were worse at fighting off viruses. In both cases, worms skewed the immune response so that the immune cells and the molecules they secreted created an environment favorable for the worm at the expense of antiviral immunity. T. A. Reese, B. S. Wakeman, H. S. Choi, M. M. Hufford, S. C. Huang, X. Zhang, M. D. Buck, A. Jezewski, A. Kambal, C. Y. Liu, G. Goel, P. J. Murray, R. J. Xavier, M. H. Kaplan, R. Renne, S. H. Speck, M. N. Artyomov, E. J. Pearce, H. W. Virgin, Helminth infection reactivates latent γ-herpesvirus via cytokine competition at a viral promoter. Science 345, 573–577 (2014). [Abstract][Full Text] L. C. Osborne, L. A. Monticelli, T. J. Nice, T. E. Sutherland, M. C. Siracusa, M. R. Hepworth, V. T. Tomov, D. Kobuley, S. V. Tran, K. Bittinger, A. G. Bailey, A. L. Laughlin, J.-L. Boucher, E. J. Wherry, F. D. Bushman, J. E. Allen, H. W. Virgin, D. Artis, Virus-helminth coinfection reveals a microbiota-independent mechanism of immunomodulation. Science 345, 578–582 (2014). [Abstract][Full Text] R. M. Maizels, W. C. Gause, How helminths go viral. Science 345, 517–518 (2014). [Abstract][Full Text]
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- 2014
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226. Women working at the state headquarters of the Alabama Equal Suffrage Association in downtown Birmingham, Alabama, during the 'Birmingham Ratification Campaign.'
- Abstract
Left to right: Mrs. A. J. Bowron, Mrs. C. B. Spencer (back), Mrs. H. E. Pearce, Mrs. Solon Jacobs (standing), Mrs. McMahon (back), Mrs. T. D. Strock, Mrs. J. R. Hornady, Mrs. W. D. Nesbitt, and Mrs. C. M. Spencer.
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- 1919
227. Women working at the state headquarters of the Alabama Equal Suffrage Association in downtown Birmingham, Alabama, during the 'Birmingham Ratification Campaign.'
- Abstract
Left to right: Mrs. A. J. Bowron, Mrs. C. B. Spencer (back), Mrs. H. E. Pearce, Mrs. Solon Jacobs (standing), Mrs. McMahon (back), Mrs. T. D. Strock, Mrs. J. R. Hornady, Mrs. W. D. Nesbitt, and Mrs. C. M. Spencer.
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- 1919
228. Studio portrait of H. Pearce, choirboy
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Dease Studios. and Dease Studios.
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Title devised by cataloguer., Taken by Dease Studios, Barrack Street, Perth., Written on back of negative: H. Pearce, name of sitter or customer, and order instructions to photographer. Found in negative box 1827 dated to 1916.
229. Finite element analyses of a wave loaded pile : deterministic and probabilistic
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Grant, C L and Pearce, H T
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GeneralLiterature_REFERENCE(e.g.,dictionaries,encyclopedias,glossaries) ,Civil Engineering - Abstract
Includes bibliography., The problem of pile stick-up, where the pile has been stabbed into the pile guide of the platform to be fixed to the sea-bed and is loaded by the current and waves of the ocean, was recently of concern when the fixation of the first local offshore production platform took place. A previous investigation considered the resonant behaviour of the undriven pile subjected to various predicted sea states and various methods were examined to limit the large displacements anticipated. In the present work, a refined model of the problem is developed using the ABAQUS finite element program. Of particular interest is the applicability of the , probabilistic finite element method (PFEM). It is important to appreciate that a mere deterministic dynamic analysis - however accurate analytically - may be of limited value when applied to structures in the ocean. The response achieved thus will either be unelegantly conservative or very unreliable due to the many uncertain parameters that pervade the field of structural dynamics in the ocean. Traditionally, statistical methods are reverted to with uncertainty analyses, but they seldom have much appeal because of the computational effort involved. The PFEM was found quite attractive with a formulation where the uncertainty of the drag coefficient and the inertia coefficient were investigated. It was concluded that the uncertainty in these coefficients should be limited to a coefficient of variation of approximately 20% and that the simplified model on which the formulation was based should be refined if acceptable results for the purposes of design were required.
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- 1991
230. Numerical analysis of cables in the offshore environment
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Davies, Graham John and Pearce, H T
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Mechanical Engineering ,InformationSystems_INFORMATIONSTORAGEANDRETRIEVAL ,ComputingMethodologies_DOCUMENTANDTEXTPROCESSING ,GeneralLiterature_REFERENCE(e.g.,dictionaries,encyclopedias,glossaries) - Abstract
Includes bibliographical references., The extraction of mineral resources from deep ocean waters has been made possible by the development of large compliant offshore structures. Mooring cables are crucial components in these offshore facilities and form the basis of this study. The aims of this thesis are: to provide a comprehensive review on all aspects of cables, to determine criteria for numerical modelling, and to ascertain the capabilities of the finite element method for cable analyses using the F.E. package ABAQUS. Difficulties associated with large sag cables arise as a result of their inherent flexibility which causes ill-conditioning of the stiffness matrices. Furthermore, the cable winding configuration causes a nonlinear stress-strain relationship, it's sagged geometry results in nonlinear strain-displacement relations, and the immersion in water leads to nonlinear fluid loadings arising from Morison's Equation as well as uncertainties in the fluid parameters. Various models, starting with the developed. Convergence difficulties basic catenary, have been at start-up, caused by a lack of stiffness in the transverse direction, are avoided by supporting the cable when applying loads. It is further established that numerical analyses of flexible structures are most stable in dynamic analyses and when under tension. In general both displacement based isoparametric and hybrid beam elements were found to be more reliable and applicable than truss elements. Cable whip, ocean floor contact and harmonic motions of cables were analysed. Finally a cable/tower interaction was modelled and subjected to a Stokes's wave. Conclusions and guidelines are presented based on the numerical experiments carried out in this study.
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- 1988
231. The finite element analysis of convection heat transfer
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Burness, Bruce Peter and Pearce, H T
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Physics::Fluid Dynamics ,Finite element method ,Heat - Transmission ,Mechanical Engineering ,Heat - Convection - Abstract
This thesis reviews the development and current methods of numerical convection heat transfer from available literature, encompassing an analysis of the various finite element formulations available for investigating convection. It further describes the finite element formulation for the primitive variable convection heat transfer equations via a Galerkin weighted residual scheme and using mixed interpolation, and it demonstrates the capability of this method by means of five practical examples, namely natural convection in a thermally driven square cavity, a thermally driven vertical slot, a thermally driven triangular cavity, and a liquid convective diode, and forced convection in a cooling pond. This study also provides the background and framework for the problem of transient convection heat transfer, and for further steady-state studies using parameters outside those considered herein.
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- 1988
232. 18F Site-Specific Labelling of a Single-Chain Antibody against Activated Platelets for the Detection of Acute Thrombosis in Positron Emission Tomography
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Ardipradja, KS, Wichmann, CW, Hickson, K, Rigopoulos, A, Alt, KM, Pearce, HA, Wang, X, O'Keefe, G, Scott, AM, Peter, K, Hagemeyer, CE, Ackermann, U, Ardipradja, KS, Wichmann, CW, Hickson, K, Rigopoulos, A, Alt, KM, Pearce, HA, Wang, X, O'Keefe, G, Scott, AM, Peter, K, Hagemeyer, CE, and Ackermann, U
- Abstract
Positron emission tomography is the imaging modality of choice when it comes to the high sensitivity detection of key markers of thrombosis and inflammation, such as activated platelets. We, previously, generated a fluorine-18 labelled single-chain antibody (scFv) against ligand-induced binding sites (LIBS) on activated platelets, binding it to the highly abundant platelet glycoprotein integrin receptor IIb/IIIa. We used a non-site-specific bio conjugation approach with N-succinimidyl-4-[18F]fluorobenzoate (S[18F]FB), leading to a mixture of products with reduced antigen binding. In the present study, we have developed and characterised a novel fluorine-18 PET radiotracer, based on this antibody, using site-specific bio conjugation to engineer cysteine residues with N-[2-(4-[18F]fluorobenzamido)ethyl]maleimide ([18F]FBEM). ScFvanti-LIBS and control antibody mut-scFv, with engineered C-terminal cysteine, were reduced, and then, they reacted with N-[2-(4-[18F]fluorobenzamido)ethyl]maleimide ([18F]FBEM). Radiolabelled scFv was injected into mice with FeCl3-induced thrombus in the left carotid artery. Clots were imaged in a PET MR imaging system, and the amount of radioactivity in major organs was measured using an ionisation chamber and image analysis. Assessment of vessel injury, as well as the biodistribution of the radiolabelled scFv, was studied. In the in vivo experiments, we found uptake of the targeted tracer in the injured vessel, compared with the non-injured vessel, as well as a high uptake of both tracers in the kidney, lung, and muscle. As expected, both tracers cleared rapidly via the kidney. Surprisingly, a large quantity of both tracers was taken up by organs with a high glutathione content, such as the muscle and lung, due to the instability of the maleimide cysteine bond in vivo, which warrants further investigations. This limits the ability of the novel antibody radiotracer 18F-scFvanti-LIBS to bind to the target in vivo and, therefore, as a useful ag
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- 2022
233. Site-Specific Glycation and Chemo-enzymatic Antibody Sortagging for the Retargeting of rAAV6 to Inflamed Endothelium
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Pearce, HA, Qian, H, Connell, TU, Huang, D, Gottstein, C, Donnelly, PS, Peter, K, Gregorevic, P, Hagemeyer, CE, Pearce, HA, Qian, H, Connell, TU, Huang, D, Gottstein, C, Donnelly, PS, Peter, K, Gregorevic, P, and Hagemeyer, CE
- Abstract
Gene therapy holds great potential for conditions such as cardiovascular disease, including atherosclerosis and also vascular cancers, yet available vectors such as the adeno-associated virus (rAAV) transduce the vasculature poorly. To enable retargeting, a single-chain antibody (scFv) that binds to the vascular cell-adhesion molecule (VCAM-1) overexpressed at areas of endothelial inflammation was site specifically and covalently conjugated to the exterior of rAAV6. To achieve conjugation, the scFv was functionalized with an orthogonal click chemistry group. This conjugation utilized site-specific sortase A methodology, thus preserving scFv binding capacity to VCAM-1. The AAV6 was separately functionalized with 4-azidophenyl glyoxal (APGO) via covalent adducts to arginine residues in the capsid's heparin co-receptor binding region. APGO functionalization removed native tropism, greatly reducing rAAV6-GFP transduction into all cells tested, and the effect was similar to the inhibition seen in the presence of heparin. Utilizing the incorporated functionalizations, the scFv was then covalently conjugated to the exterior of rAAV6 via strain-promoted azide-alkyne cycloaddition (SPAAC). With both the removal of native heparin tropism and the addition of VCAM-1 targeting, rAAV6 transduction of endothelial cells was greatly enhanced compared to control cells. Thus, this novel and modular targeting system could have further application in re-directing AAV6 toward inflamed endothelium for therapeutic use.
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- 2019
234. Low-Fouling and Biodegradable Protein-Based Particles for Thrombus Imaging
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Bonnard, T, Jayapadman, A, Putri, JA, Cui, J, Ju, Y, Carmichael, C, Angelovich, TA, Cody, SH, French, S, Pascaud, K, Pearce, HA, Jagdale, S, Caruso, F, Hagemeyer, CE, Bonnard, T, Jayapadman, A, Putri, JA, Cui, J, Ju, Y, Carmichael, C, Angelovich, TA, Cody, SH, French, S, Pascaud, K, Pearce, HA, Jagdale, S, Caruso, F, and Hagemeyer, CE
- Abstract
Nanomedicine holds great promise for vascular disease diagnosis and specific therapy, yet rapid sequestration by the mononuclear phagocytic system limits the efficacy of particle-based agents. The use of low-fouling polymers, such as poly(ethylene glycol), efficiently reduces this immune recognition, but these nondegradable polymers can accumulate in the human body and may cause adverse effects after prolonged use. Thus, new particle formulations combining stealth, low immunogenicity and biocompatible features are required to enable clinical use. Here, a low-fouling particle platform is described using exclusively protein material. A recombinant protein with superior hydrophilic characteristics provided by the amino acid repeat proline, alanine, and serine (PAS) is designed and cross-linked into particles with lysine (K) and polyglutamic acid (E) using mesoporous silica templating. The obtained PASKE particles have low-fouling behavior, have a prolonged circulation time compared to albumin-based particles, and are rapidly degraded in the cell's lysosomal compartment. When labeled with near-infrared fluorescent molecules and functionalized with an anti-glycoprotein IIb/IIIa single-chain antibody targeting activated platelets, the particles show potential as a noninvasive molecular imaging tool in a mouse model of carotid artery thrombosis. The PASKE particles constitute a promising biodegradable and versatile platform for molecular imaging of vascular diseases.
- Published
- 2018
235. BTS Recommendations to Respiratory Physicians for Organising the Care of Patients with Lung Cancer
- Published
- 1998
236. Helicobacter pylori antibody titres in serum, plasma and successively thawed specimens: implications for epidemiological and clinical studies
- Author
-
Pearce, D C, Peach, H G, and Farish, S J
- Published
- 1996
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