400 results on '"Olivier Soubrane"'
Search Results
352. Efficient suicide gene therapy of transduced and distant untransduced ovary tumors is correlated with significant increase of intratumoral T and NK cells
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Olivier Soubrane, Didier Houssin, Monique Fabre, Yves Panis, Helena Nagy, Carsten Engelmann, and David Klatzmann
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Ganciclovir ,medicine.medical_specialty ,Genetic enhancement ,T-Lymphocytes ,Ovary ,Herpesvirus 1, Human ,Biology ,Thymidine Kinase ,Natural killer cell ,Internal medicine ,medicine ,Bystander effect ,Tumor Cells, Cultured ,Animals ,Lymphocyte Count ,Rats, Wistar ,Pharmacology ,Ovarian Neoplasms ,General Medicine ,Genetic Therapy ,Suicide gene ,Immunohistochemistry ,Rats ,Killer Cells, Natural ,medicine.anatomical_structure ,Endocrinology ,Thymidine kinase ,Cancer research ,Female ,medicine.drug - Abstract
Gene therapy using herpes simplex type 1 thymidine kinase gene (HSV1-TK) transfer followed by ganciclovir (GCV) treatment has revealed an important intratumoral and regional bystander effect that is at least partly immune-mediated. The aim of this work was to study the modifications of T lymphocyte subpopulations in a model of distant bystander effect occurring between ovary tumors. Bilateral ovarian tumors were generated in 21 WKY rats by injection in the ovarian pouch of either parental or HSV1-TK-expressing DWA-OC-1 ovarian cancer cells. After 14 days, rats were treated for two weeks with GCV (75 mg/kg x 2/d) or saline. All rats were killed at day 29 for pathological examination. The tumor-infiltrating mononuclear cells were analyzed by semi-quantitative immunohistochemistry. As compared to rats receiving saline, GCV-treated animals exhibited a complete disappearance of the HSV1-TK+ tumors with residual fibrotic scars (ovary weights: 0.46 +/- 0.4 g vs 10.11 +/- 1.5 g, P0.001). Interestingly, the contralateral HSV1-TK negative tumor showed a significant regression (12.39 +/- 1.93 g vs 22.24 +/- 237 g, P0.014). Furthermore, a lower incidence of tumoral ascitis was found in the GCV-receiving group (20% vs 90% P0.02). Within both TK- and TK+ tumors, there was a significant increase of CD4+, CD8+ and NK cells in the GCV-treated group compared to the saline-treated group. This study thus indicates that a distant bystander effect not only acts between close tumors within a given organ such as the liver, but also between more distant tumors in the peritoneal cavity. This effect is associated with significant infiltration of the tumor by immune system cells, supporting the notion that the distant bystander effect is immune-mediated.
- Published
- 2000
353. A preclinical model of hepatocyte gene transfer: the in vivo, in situ perfused rat liver
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Majid Mehtali, Monique Fabre, D Houssin, Olivier Soubrane, Claudia Mitchell, R Malafosse, and J L De Godoy
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Male ,viruses ,Transgene ,Genetic Vectors ,Gene Expression ,Biology ,Polymerase Chain Reaction ,Statistics, Nonparametric ,Metabolic Diseases ,In vivo ,Genetics ,medicine ,Animals ,Molecular Biology ,Splanchnic Circulation ,Genetic transfer ,Gene Transfer Techniques ,Genetic Therapy ,Provirus ,beta-Galactosidase ,Molecular biology ,Liver Regeneration ,Rats ,Perfusion ,medicine.anatomical_structure ,Retroviridae ,Cell culture ,Rats, Inbred Lew ,Hepatocyte ,Immunology ,Models, Animal ,Hepatocytes ,Molecular Medicine - Abstract
Delivering retroviruses targeted to hepatocytes in vivo involves the injection of retroviruses directly into the portal vein. The aim of this work was to establish a clinically relevant system for retrovirus-mediated gene transfer in a new model of in vivo, in situ perfused rat liver and to study the transgene expression. At 24 h after partial hepatectomy, the liver was completely excluded from the splanchnic circulation using an extracorporeal shunt. Two independent normothermal, oxygenated perfusion systems were used. First, liver perfusion was carried out with a recirculating system (1 h). Culture supernatant containing retroviruses (1.5 x 10(8) ffu/ml, beta-galactosidase gene) was used as perfusate. Then the liver perfusion was maintained for more 30 min in a single liver passage system using culture medium without retroviruses as perfusate. High hepatocyte transduction rates (up to 34.4%) were obtained. PCR analysis showed no provirus in extrahepatic organs. Viral titrations performed simultaneously (inflow and outflow liver lines) showed that after 1 h of perfusion (up to 30 successive liver passages) retroviruses were still detected in the liver outflow perfusate (up to 2.0 x 10(7) ffu/ml). Washing the liver for 30 min dramatically decreased the leakage of retroviruses in the outflow. In order to be of clinical use, the injection of retroviruses targeted to hepatocytes in vivo should be done while the liver is completely excluded from the splanchnic circulation to avoid any extrahepatic retrovirus diffusion.
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- 2000
354. In vivo hepatocyte retrovirus-mediated gene transfer through the rat biliary tract
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Jose Luiz De Godoy, Olivier Soubrane, Didier Houssin, Majid Mehtali, Monique Fabre, and Robert Malafosse
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DNA Replication ,Male ,Pathology ,medicine.medical_specialty ,viruses ,Portal vein ,Gene transfer ,Polymerase Chain Reaction ,Cell Line ,Retrovirus ,In vivo ,Genetics ,medicine ,Animals ,Hepatectomy ,Transgenes ,Biliary Tract ,Molecular Biology ,DNA Primers ,biology ,Base Sequence ,Gene Transfer Techniques ,biology.organism_classification ,beta-Galactosidase ,Liver Regeneration ,Rats ,medicine.anatomical_structure ,Retroviridae ,Liver ,Biliary tract ,Rats, Inbred Lew ,Hepatocyte ,Molecular Medicine ,Blood stream - Abstract
Delivering retroviruses targeted to hepatocytes in vivo involves the injection of retroviruses directly into the blood stream of the portal vein. The aim of this work was to delineate the conditions for delivering retroviruses in vivo by perfusing in situ the bile duct of the regenerating rat liver, and to study the hepatocyte transgene expression. At 24 hr after partial hepatectomy, during the S phase of the cell cycle, regenerating livers were perfused for 2.8+/-0.5 hr through the bile duct with 36.2+/-6.8 ml (0.3+/-01 ml/min) of fresh culture supernatant containing amphotropic recombinant retroviruses encoding the beta-galactosidase gene. The virus total titer was 1.5 x 10(8) ffu (group I) or 6.5 x 10(8) ffu (groups II and III). The hepatic artery blood flow was either maintained (groups I and II) or interrupted (group III) during bile duct perfusion. Liver biopsies taken 7 days later showed that 31.4+/-24.2% (group I), 58.7+/-23.6% (group II), and 45.1+/-21.4% (group III) of hepatocytes expressed beta-galactosidase activity, predominantly in the periportal and mediolobular zones. This study demonstrates that hepatocytes of regenerating rat livers that have entered the S phase of the cell cycle as a result of partial hepatectomy can be transduced in vivo by retroviral vectors delivered in situ by bile duct perfusion. Furthermore, the number of transduced hepatocytes closely correlated with the viral total titer and was diminished by hepatic artery blood flow occlusion during perfusion.
- Published
- 1999
355. Rationale and technical constraints of a tertiary liver transplantation
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Pierre-Philippe Massault, Yves Ozier, Olivier Bernard, Joël Pitre, Denis Devictor, Bertrand Dousset, Didier Houssin, and Olivier Soubrane
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Adult ,medicine.medical_specialty ,Vena cava ,medicine.medical_treatment ,Portal vein ,Economic shortage ,Liver transplantation ,Resection ,Hepatolenticular Degeneration ,Biliary Atresia ,medicine.artery ,Medicine ,Humans ,Aorta ,Hepatology ,business.industry ,Infant ,Surgery ,Liver Transplantation ,Hepatitis, Autoimmune ,medicine.anatomical_structure ,Child, Preschool ,Severe morbidity ,Emergencies ,business ,Artery - Abstract
Because of the current shortage of donor organs, the routine performance of tertiary liver transplantation (LT) may be questioned. In this study, the indications of tertiary LT are discussed, paying particular attention to intraoperative technique. Of 501 LTs performed from 1986 to 1995, eight (1.6%) were tertiary LTs. Three patients underwent an emergent third LT because of associated hepatic artery and portal vein thromboses (n = 2) or hyperacute rejection (n = 1). Five patients had an elective third LT for ischemic cholangitis (n = 4) or chronic rejection (n = 1). The 3 patients who underwent retransplantation emergently died early from multiple-organ failure. Because of previous surgery and subsequent technical difficulties, the third LT in the remaining 5 patients required unroutine surgical procedures including the following: intrapericardial control of the suprahepatic vena cava (n = 1), "en bloc" clamping of both the infrahepatic vena cava and the hepatic pedicle (n = 1), arterial reconstruction onto the aorta via an aortoiliac conduit (n = 5), and aortic resection with aortoaortic prosthetic reconstruction (n = 1). Of these 5 patients, 4 required reoperation because of bowel perforation (n = 5) or intraperitoneal bleeding (n = 1). The 5 patients (62%) who were regrafted electively are alive and well after a median follow-up of 45 months. A third LT can be reasonably offered to selected young recipients if performed electively. Tertiary LT may require unroutine surgical procedures and may lead to severe morbidity. (Liver Transpl Surg 1997 Nov;3(6):624-7)
- Published
- 1997
356. Common bile duct stenosis caused by chronic pancreatitis after liver transplantation for alcoholic cirrhosis
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J Pitre, Pierre-Philippe Massault, Didier Houssin, Olivier Soubrane, Bertrand Dousset, Yvon Calmus, and S Benoist
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Adult ,Male ,Alcoholic liver disease ,medicine.medical_specialty ,Cirrhosis ,Pancreatic disease ,Time Factors ,medicine.medical_treatment ,Common Bile Duct Diseases ,Temperance ,Constriction, Pathologic ,Liver transplantation ,Gastroenterology ,Liver Cirrhosis, Alcoholic ,Internal medicine ,medicine ,Prevalence ,Humans ,Transplantation ,Common bile duct ,business.industry ,Middle Aged ,medicine.disease ,Liver Transplantation ,Stenosis ,medicine.anatomical_structure ,Pancreatitis ,Chronic Disease ,business - Abstract
Background The prevalence of chronic pancreatitis in patients with alcoholic cirrhosis ranges from 7% to 11% and is not considered a contraindication for liver transplantation. Methods Among 59 liver transplant recipients grafted for alcoholic cirrhosis, we report two observations of common bile duct stenosis due to chronic pancreatitis. Results In both cases, pretransplant work-up disclosed no clinical or radiological evidence of chronic pancreatitis. The diagnosis of common bile duct stricture was made 6 and 60 months after liver transplantation. One patient was reoperated upon, and his choledochocholedochostomy was converted into a Rouxen-Y choledochojejunostomy. The second patient experienced metastatic laryngeal carcinoma and died before reoperation. Conclusions These observations suggest that common bile duct stricture caused by chronic pancreatitis may occur after liver transplantation for alcoholic cirrhosis, even after a long-standing history of abstinence.
- Published
- 1997
357. Protein-losing enteropathy secondary to hepatic venous outflow obstruction after liver transplantation
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Didier Houssin, Olivier Soubrane, Daniel Couturier, Paul Legmann, Stanislas Chaussade, Bertrand Dousset, and Yvon Calmus
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Adult ,Male ,medicine.medical_specialty ,medicine.medical_treatment ,Protein-Losing Enteropathies ,Constriction, Pathologic ,Liver transplantation ,Budd-Chiari Syndrome ,Hepatic Veins ,Gastroenterology ,Primary sclerosing cholangitis ,Angioplasty ,Internal medicine ,medicine ,Humans ,Enteropathy ,Hepatology ,business.industry ,Protein losing enteropathy ,medicine.disease ,Surgery ,Liver Transplantation ,Transplantation ,Radiography ,Stenosis ,Budd–Chiari syndrome ,Venae Cavae ,business ,Angioplasty, Balloon - Abstract
A 42-year-old man with a history of repeated abdominal surgery and lymph node tuberculosis underwent orthotopic liver transplantation for primary sclerosing cholangitis. Two years after transplantation, this patient developed a severe protein-losing enteropathy with no evidence of cardiac disease or lymphoproliferative disorder. Imaging work-up revealed hemodynamically significant stenosis of the supra-hepatic caval anastomosis, which was treated by percutaneous balloon angioplasty. All clinical and biochemical disorders resolved within 1 month after percutaneous dilatation, but relapsed simultaneously with recurrent anastomotic stenosis 15 months later. Repeat caval angioplasty resulted in rapid recovery, which strongly suggests that hepatic venous outflow obstruction was responsible for the protein-losing enteropathy in this patient.
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- 1997
358. Polyamines inhibit lipopolysaccharide-induced nitric oxide synthase activity in rat liver cytosol
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François Blachier, Olivier Soubrane, Alexandre Mignon, Laboratoire de nutrition et sécurité alimentaire, Institut National de la Recherche Agronomique (INRA), and ProdInra, Migration
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Lipopolysaccharides ,Cancer Research ,Arginine ,Physiology ,[SDV]Life Sciences [q-bio] ,Clinical Biochemistry ,Spermine ,Nitric Oxide Synthase Type II ,Biochemistry ,Nitric oxide ,chemistry.chemical_compound ,Cytosol ,Polyamines ,Animals ,Enzyme inducer ,biology ,Chemistry ,Molecular biology ,Rats ,[SDV] Life Sciences [q-bio] ,Nitric oxide synthase ,Spermidine ,Liver ,Enzyme Induction ,biology.protein ,Putrescine ,Nitric Oxide Synthase - Abstract
Liver cells can produce nitric oxide from L-arginine through either constitutive NO synthase or inducible NO synthase (NOS) detected after in vivo or in vitro treatment with cytokines and/or lipopolysaccharide (LPS). The effects of NO on liver cells are associated with protein synthesis and mitochondrial electron transfer inhibition. L-Arginine is also the precursor of L-ornithine and polyamines. The latter are considered to be protective in the liver in several experimental models. The aim of the present work was to test the effects of polyamines on LPS-inducible NOS activity in rat liver cytosol using the test of radioactive L-citrulline synthesis from L-[guanido-14C]arginine. The three polyamines inhibited inducible NO synthase activity with the following hierarchy: spermine > spermidine approximately equal to putrescine. The 0.5 mM spermine was found to inhibit 50% of inducible NO synthase activity. The present data suggest an inhibitory interrelationship in the liver between two metabolites derived from the common precursor L-arginine.
- Published
- 1997
359. Metastatic endocrine tumors: medical treatment, surgical resection, or liver transplantation
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Olivier Soubrane, Bertrand Dousset, Olivier Saint-Marc, J Pitre, Didier Houssin, and Yves Chapuis
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Adult ,Male ,medicine.medical_specialty ,Antimetabolites, Antineoplastic ,Palliative care ,Antineoplastic Agents, Hormonal ,medicine.medical_treatment ,Carcinoid Tumor ,Liver transplantation ,Disease-Free Survival ,Streptozocin ,Actuarial Analysis ,Antineoplastic Combined Chemotherapy Protocols ,medicine ,Hepatectomy ,Humans ,Chemoembolization, Therapeutic ,Survival rate ,Aged ,Chemotherapy ,Antibiotics, Antineoplastic ,business.industry ,Contraindications ,Liver Neoplasms ,Palliative Care ,Age Factors ,Vascular surgery ,Middle Aged ,Surgery ,Liver Transplantation ,Survival Rate ,Neuroendocrine Tumors ,Cardiothoracic surgery ,Female ,Fluorouracil ,Neoplasm Recurrence, Local ,business ,Abdominal surgery ,Follow-Up Studies - Abstract
We reviewed our experience with 34 patients with metastatic endocrine tumors (METs) who were treated by different modalities. Eight patients were treated by chemotherapy or chemoembolization because of stable disease or surgical contraindications. Seventeen patients underwent curative or cytoreductive surgical resection. Nine patients received grafts based on the following criteria: no extrahepatic spread on imaging workup and nonresectable symptomatic metastatic disease. Of the eight medically treated patients, the five patients with initial stable clinical condition are alive 32 to 56 months after referral. Of the 17 patients treated by liver resection, 13 are alive 6 to 108 months after surgery, and 7 are disease-free. After curative resection, the 5-year actuarial survival and disease-free survival rates were 62% and 52%, respectively. Of the nine grafted patients, three patients grafted for carcinoid tumor are alive at 15, 24, and 62 months, one of whom has a late recurrence. Our results indicate that therapeutic indications for METs should be based on age, clinical symptoms, histologic type, and tumor extension: Patients with stable MET may benefit from surgical restraint; liver resection in patients with aggressive MET may provide good long-term palliation and possibly cure one-third of the patients; liver transplantation should be restricted to young patients with nonresectable carcinoid MET but remains a high-risk operation because of previous surgery and chemoembolization.
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- 1996
360. How valid is emergency liver transplantation for acute liver necrosis in patients with multiple-organ failure?
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Yves Chapuis, Joël Pitre, Olivier Soubrane, Denis Devictor, Bertrand Dousset, François Baudin, Yves Ozier, Didier Houssin, and Olivier Bernard
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Adult ,Male ,medicine.medical_specialty ,Adolescent ,medicine.medical_treatment ,Multiple Organ Failure ,Apache II score ,Context (language use) ,Liver transplantation ,law.invention ,Liver necrosis ,Necrosis ,law ,Medicine ,Humans ,In patient ,Primary graft failure ,Child ,Aged ,Retrospective Studies ,Hepatology ,business.industry ,Infant ,Middle Aged ,Intensive care unit ,Surgery ,Liver Transplantation ,Treatment Outcome ,Liver ,Health evaluation ,Child, Preschool ,Acute Disease ,Female ,Emergencies ,business - Abstract
Multiple-organ failure (MOF), defined as the failure of initially uninvolved organs, is the final step of definitive and massive liver necrosis. Emergency liver transplantation (ELT) has radically modified the outcome of acute liver failure and early primary graft failure, but the results of ELT in cases of MOF are unknown. From May 1988 to June 1993, 243 patients underwent a liver transplantation (LT). Thirty-seven patients (15.2%) who had an acute liver necrosis complicated by a MOF underwent an ELT. Twenty-one patients were children. An emergency retransplantation was performed in 16 patients. Three or 4 organ-system failures (OSF) were present in 13 patients. Before ELT, the mean Acute Physiology and Chronic Health Evaluation (APACHE) II score was 26.3 ± 5.1. Six-month and 1-year survival rates were 37.8% and 25.9%, respectively, after ELT complicated by MOF, and 78% and 73.5%, respectively, in other cases of LT. Twenty-six patients had surgical complications (70%), whereas thirty-one patients had medical complications (84%). Twentytwo patients died during the postoperative period (60%). Before ELT, infection (P < .05), cardiovascular failure (P < .03), and more than two OSF (P < .05) were more frequent in patients who died after intervention. The APACHE II score (P < .05) and the length of stay in the intensive care unit before ELT (P < .05) were lower among survivors. In the context of liver allograft shortage, our results suggest that an ELT should not be performed in patients with cardiac failure, more than two OSF, or an APACHE II score higher than 30. Copyright © 1996 by the American Association for the Study of Liver Diseases.
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- 1996
361. SOS (Syndrome d’Obstruction des Sinusoïdes) après chimiothérapie pour métastases de cancers colo-rectaux : poursuite d’un coupable
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P. Sartoretti, M Le Charpentier, Stanislas Chaussade, C. Brezault, Olivier Soubrane, Arnaud Roth, Gilles Mentha, Virginie Audard, Benoit Terris, Laura Rubbia-Brandt, Philippe Morel, and Bertrand Dousset
- Subjects
Pathology and Forensic Medicine - Abstract
Buts du travail Avec environ 34 000 nouveaux cas par an, le cancer colorectal (CCR) est le cancer digestif le plus frequent dans la population francaise. Le risque de survenue de metastase est eleve avec au premier rang les metastases hepatiques. La chimiotherapie systemique neo-adjuvante represente un des traitements les plus prometteurs pour augmenter le succes de la chirurgie des metastases hepatiques des CCR. Si cliniquement, l’efficacite de nouveaux cytotoxiques comme l’oxaliplatine ou l’irinotecan dans le traitement des metastases hepatiques de CCR a ete demontree, une eventuelle toxicite de ces derniers n’a pas ete analyse au niveau du foie non tumoral. Methodes Nous avons etudie retrospectivement l’aspect morphologique du foie non tumoral de 153 resections de metastases hepatiques de CCR effectuees entre Janvier 1994 et Decembre 2002 a l’hopital Cochin et a l’hopital de Geneve. Quatre vingt sept malades avaient ete prealablement traites par chimiotherapie avant la resection chirurgicale. Differents criteres ont ete evalues comme l’architecture hepatique, la steatose et la presence de lesions vasculaires. Une etude en microscopie electronique du foie non tumoral a egalement ete effectuee dans 3 cas. Resultats Des dilatations sinusoidales centro-lobulaires, d’intensite parfois marquee, etaient observees chez 44 des 83 malades (51 %) traites prealablement par chimiotherapie. Ces anomalies etaient associees dans 50 % des cas a une fibrose sinusoidale et des veines centro-lobulaires. Dans 7 cas, des lesions d’hyperplasie nodulaire regenerative etaient observees. L’etude en microscopie electronique confirmait l’existence d’une atteinte de la barriere sinusoidale. Ces lesions etaient significativement correlees a l’utilisation de l’oxaliplatine avec 34 des 43 malades (78 %) presentant cette atteinte vasculaire (p Conclusion La chimiotherapie systemique pre-operatoire des metastases de CCR induit de frequentes alterations vasculaires hepatiques similaires a celles observees au cours de la maladie veino-occlusive ou Syndrome d’Obstruction des Sinusoides (SOS). Ces lesions devraient etre incluses dans la liste des effets secondaires de la chimiotherapie, en particulier en relation avec l’utilisation de l’oxaliplatine. Les consequences cliniques de ces alterations vasculaires sont a evaluer d’autant que certains preconisent l’utilisation de l’oxaliplatine en chimiotherapie adjuvante des CCR au stade 3.
- Published
- 2004
362. Combined lung and liver transplantation in patients with cystic fibrosis. A 4 1/2-year experience
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Olivier Soubrane, Bertrand Dousset, Jean Paul Couetil, Antoine Achkar, Didier Houssin, Alain Carpentier, Michael Tolan, Didier F. Loulmet, Alain Guinvarch, Catherine Amrein, Romain Guillemain, P. Chevalier, and Peter L. Birnbaum
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Pulmonary and Respiratory Medicine ,Adult ,Liver Cirrhosis ,Male ,Reoperation ,medicine.medical_specialty ,Cirrhosis ,Adolescent ,Cystic Fibrosis ,Heart-Lung Transplantation ,medicine.medical_treatment ,Vital Capacity ,Liver transplantation ,Postoperative Complications ,Forced Expiratory Volume ,Ascites ,medicine ,Lung transplantation ,Aspergillosis ,Humans ,Pseudomonas Infections ,Child ,Immunosuppression Therapy ,Postoperative Care ,business.industry ,Respiratory disease ,Immunosuppression ,medicine.disease ,Tissue Donors ,Surgery ,Liver Transplantation ,Transplantation ,Female ,medicine.symptom ,business ,Cardiology and Cardiovascular Medicine ,Respiratory Insufficiency ,Lung Transplantation - Abstract
Patients with cystic fibrosis who have end-stage respiratory failure and associated liver cirrhosis have been considered poor candidates for lung transplantation because of high morbidity and mortality resulting from hepatic insufficiency after the operation. Since April 1989, our policy has been to combine heart-lung or lung and liver transplantation in this group of patients. Between June 1990 and March 1995, among 25 patients accepted in the program for combined transplantation, nine died awaiting transplantation and 10 underwent one of the following procedures: heart-lung-liver transplantation ( n = 5), en bloc double lung–liver transplantation ( n = 1), sequential double lung–liver transplantation ( n = 3), and bilateral lobar lung transplantation from a split left lung and reduced liver transplantation ( n = 1). There were 5 male and 5 female patients. The ages of the recipients ranged from 10 to 24 years. Mean forced expiratory volume in 1 second was 29% and mean forced vital capacity was 35% of predicted values. All patients were infected with resistant Pseudomonas, three with Pseudomonas cepaceia, and two patients had Aspergillus species in addition. All patients had severe cirrhosis with portal hypertension. Four patients had a history of esophageal variceal bleeding and two had had previous portosystemic shunts. The operation was performed as a two-stage procedure, the intrathoracic operation being completed before the abdominal stage was begun. Cardiopulmonary bypass was used in all patients because of poor clinical condition. Immunosuppression consisted of azathioprine, cyclosporine, and prednisone, as for isolated lung transplantation. There were two perioperative deaths, one caused by primary liver failure and the second by early lung dysfunction. For the first 3 months after transplantation pulmonary infection was the most common cause of morbidity. Other complications included tracheal stenosis ( n = 1), bronchial stenosis ( n = 1), biliary stricture ( n = 2), and severe ascites ( n = 3). All were successfully treated. Obliterative bronchiolitis developed in three patients. This was stabilized with FK 506 in two patients; the other patient underwent retransplantation at 38 months but eventually died of bleeding. Actuarial survival was 70% at 1 year and remained unchanged at 3 years. Significant functional improvement was observed in all survivors. For patients who have chronic respiratory failure with advanced cirrhosis, lung transplantation combined with liver transplantation can be performed with a satisfactory outcome. (J THORAC CARDIOVASC SURG 1995;110:1415-23)
- Published
- 1995
363. Short-term cardiovascular effects of somatostatin in patients with cirrhosis
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Olivier Soubrane, Christophe Gaudin, Bruno Champigneulle, Richard Moreau, Didier Lebrec, and Gerhard Kleber
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Adult ,Liver Cirrhosis ,Male ,medicine.medical_specialty ,Cardiac output ,Cirrhosis ,Time Factors ,Octreotide ,Hemodynamics ,Blood Pressure ,Bolus (medicine) ,Heart Rate ,medicine.artery ,Internal medicine ,medicine ,Humans ,Infusions, Intravenous ,Hepatology ,business.industry ,Middle Aged ,medicine.disease ,Endocrinology ,Blood pressure ,Somatostatin ,Anesthesia ,Pulmonary artery ,Injections, Intravenous ,Female ,business ,medicine.drug - Abstract
Somatostatin is used to treat variceal hemorrhage in patients with cirrhosis and portal hypertension. Its systemic hemodynamic effects, however, are not yet well defined. Since cardiomyopathy or pulmonary artery hypertension may occur in patients with cirrhosis, definition of the systemic hemodynamic effects of somatostatin or its analogue octreotide is of clinical importance. The aim of this study was to evaluate the effects of somatostatin, at different doses and under different conditions of administration, on the systemic hemodynamics in 17 patients with cirrhosis. Two sets of experiments were performed. In the first, eight patients received two different bolus doses (100 and 250 micrograms) of somatostatin. The second set of experiments was designed to study the hemodynamic effects of the combination of a bolus and an infusion of somatostatin. Nine other patients received one bolus of 250 micrograms of somatostatin, followed by a 250 micrograms/h infusion for 65 min. A second bolus of 250 micrograms of somatostatin was injected in these patients after 35 min of infusion. Before and for 30 min after each bolus, systemic hemodynamics were measured. Following a bolus of somatostatin, a dose-dependent decrease in heart rate (from 77 +/- 3 to 73 +/- 5 beats/min with 100 micrograms, and from 78 +/- 4 to 68 +/- 5 beats/min with 250 micrograms, p < 0.05) and increases in systemic and pulmonary artery pressures were observed. The combination of an infusion and a bolus of somatostatin significantly reduced the increases in systemic and pulmonary artery pressures.(ABSTRACT TRUNCATED AT 250 WORDS)
- Published
- 1995
364. Evidence for the detrimental role of proteolysis during liver preservation in humans
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Luc Cynober, Bertrand Dousset, Yvon Calmus, Didier Houssin, Filomena Conti, Olivier Soubrane, Jacqueline Giboudeau, and Soo Kyung Lim
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Adult ,Male ,medicine.medical_specialty ,Adenosine ,Allopurinol ,Organ Preservation Solutions ,Cold storage ,Biology ,chemistry.chemical_compound ,Raffinose ,Predictive Value of Tests ,Internal medicine ,medicine ,Humans ,Insulin ,Aspartate Aminotransferases ,Prospective Studies ,Amino Acids ,Liver preservation ,Alanine ,chemistry.chemical_classification ,Methionine ,Hepatology ,Graft Survival ,Gastroenterology ,Proteins ,Alanine Transaminase ,Organ Preservation ,Ornithine ,Glutathione ,Amino acid ,Liver Transplantation ,Transplantation ,Cold Temperature ,Endocrinology ,Treatment Outcome ,chemistry ,Biochemistry ,Liver ,Female ,Leucine - Abstract
Background/Aims Proteolysis may persist in the liver aliograft during cold storage. The aim of this study was to determine the significance of proteolysis within liver allografts stored at 4°C in University of Wisconsin preservation fluid. Methods Thirty recipients of 32 liver allografts were studied prospectively. Amino acid content of the preservation fluid was analyzed at the end of cold storage and was correlated to graft and patient outcome after transplantation. Results Analysis of the preservation fluid showed the presence of free amino acids, the profile of which was different from that of stored liver parenchyma. Concentrations of amino acids (alanine, cysteine, leucine, isoleucine, methionine, lysine, ornithine, and threonine) and transaminases (alanine aminotransferase and aspartate aminotransferase) in the preservation fluid correlated with the duration of cold ischemia. Indexes of graft dysfunction (serum alanine aminotransferase and aspartate aminotransferase peaks and prothrombin rate) correlated with concentrations of cysteine, alanine, isoleucine, leucine, methionine, lysine, ornithine, and threonine, whereas enzyme concentrations in the fluid were not predictive of graft dysfunction. Conclusions These data suggest that liver proteolysis occurs during cold storage and may have a detrimental effect on the outcome after transplantation. The measurement of the amino acids in the preservation fluid at the end of the cold storage period could help to identify the most severely damaged organs.
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- 1995
365. The 'right place' for the left liver…
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Olivier Soubrane and Olivier Scatton
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medicine.medical_specialty ,Hepatology ,business.industry ,Gastroenterology ,medicine ,Left liver ,business ,Surgery - Published
- 2012
366. Liver transplantation for end-stage liver disease associated with alpha-1-antitrypsin deficiency in children: pretransplant natural history, timing and results of transplantation
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Franco Filipponi, Olivier Soubrane, Jacques Valayer, Olivier Bernard, Denis Devictor, Didier Houssin, and Françoise Labrousse
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Male ,medicine.medical_specialty ,Cirrhosis ,Time Factors ,medicine.medical_treatment ,Hepatosplenomegaly ,Liver transplantation ,Gastroenterology ,Liver disease ,Internal medicine ,alpha 1-Antitrypsin Deficiency ,medicine ,Humans ,Child ,Retrospective Studies ,Alpha 1-antitrypsin deficiency ,Hepatology ,business.industry ,Jaundice ,medicine.disease ,Surgery ,Liver Transplantation ,Transplantation ,Phenotype ,Treatment Outcome ,Female ,Liver function ,medicine.symptom ,business ,Liver Failure ,Metabolism, Inborn Errors ,Follow-Up Studies - Abstract
Alpha-1-antitrypsin deficiency is an inborn metabolism error which can cause emphysema and liver disease. As regards the pathophysiology of liver disease, this deficiency is poorly understood, and it is also not known why only a small proportion of Pi ZZ individuals progress towards cirrhosis and liver failure. Since there is no specific therapy for end-stage liver disease associated with alpha-1-antitrypsin deficiency, patients are considered candidates for liver transplantation. In this paper, the natural history of 16 children who underwent liver transplantation is reviewed. Fourteen patients had neonatal cholestasis as a first symptom of the disease and hepatosplenomegaly was present in all children by the age of 12 months. In 11 children, jaundice recurred, always with liver function deterioration. Two patients had a histological paucity of interlobular bile ducts and required early transplantation due to rapid progression of liver failure. At the time of pretransplant assessment, all the patients in this study had portal hypertension and seven of them had experienced at least one episode of gastrointestinal bleeding. One child had moderate intrapulmonary shunts with hypoxemia, but the others had normal spirometry and blood gases. There was no other extrahepatic complication of alpha-1-antitrypsin deficiency. Eighteen orthotopic liver transplantations were performed in 16 patients. One patient died 8 days after retransplantation due to graft necrosis. Fifteen patients (94%) were alive after a median follow-up of 22 months with an excellent quality of life, normal serum alpha-1-antitrypsin levels and without evidence of liver disease recurrence or pulmonary complications. Liver disease related to alpha-1-antitrypsin deficiency should be diagnosed early; infants with jaundice persisting after the age of 6 months and older patients, as well as patients who have experienced recurrent jaundice, prolonged prothrombin time and/or development of portal hypertension, should be referred to a transplant center for assessment. In most cases, liver disease evolves slowly, but liver transplantation should be performed before the onset of possible extrahepatic complications.
- Published
- 1994
367. Controlled liver splitting for transplantation in two recipients: technique, results and perspectives
- Author
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Yves Chapuis, Olivier Boillot, Olivier Bernard, Y Ozier, Didier Houssin, C Couinaud, Olivier Soubrane, Denis Devictor, and J Pitre
- Subjects
Adult ,Graft Rejection ,Male ,medicine.medical_specialty ,Adolescent ,medicine.medical_treatment ,Blood Loss, Surgical ,Liver transplantation ,Revascularization ,Cholangiography ,Hematoma ,Postoperative Complications ,medicine ,Humans ,Child ,Survival rate ,Vascular Patency ,medicine.diagnostic_test ,business.industry ,Infant ,Middle Aged ,medicine.disease ,Surgery ,Portal vein thrombosis ,Liver Transplantation ,Transplantation ,surgical procedures, operative ,medicine.anatomical_structure ,Liver ,Child, Preschool ,Female ,Radiology ,business ,Artery - Abstract
A technique of controlled liver splitting for transplantation in two recipients is proposed, based on a full anatomical assessment of the graft including arteriography and cholangiography on the back-table. Using eight livers, 16 patients received a graft: right liver (eight patients), left lobe (four) or left liver (four). Twelve patients required urgent or very urgent transplantation. Anatomical assessment of the graft demonstrated a portal bifurcation in all cases, a common trunk of the left and middle hepatic veins in five, a right biliary duplication in three and duplication of the left branch of the middle hepatic artery in one. After revascularization of the graft, bleeding was greater in patients with a right graft, particularly if the middle hepatic vein had been ligated. The main postoperative complications were hepatic artery thrombosis (four cases), biliary complications (four), portal vein thrombosis (two), haematoma (two) and abscess (two). No primary non-function of the graft was observed. The postoperative survival rate was 75 per cent. The four patients in whom transplantation was not considered urgent are still alive. The immediate survival rate of the grafts was 69 per cent. These results compare favourably with those in the literature. In spite of the technical, logistical and ethical problems raised by this technique, the results suggest that controlled liver splitting for transplantation in two recipients may in the future significantly improve the feasibility of liver transplantation.
- Published
- 1993
368. Hepatitis E–associated subacute liver failure: a rare indication for liver transplantation
- Author
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João Eduardo Nicoluzzi, Stanislas Chaussade, Yvon Calmus, Olivier Soubrane, Philippe Sogni, Jorge Cardoso, and Didier Mennecier
- Subjects
Pathology ,medicine.medical_specialty ,Hepatology ,business.industry ,medicine.medical_treatment ,Gastroenterology ,medicine ,Liver failure ,Liver transplantation ,business ,Hepatitis E ,medicine.disease - Published
- 2001
369. Hemodynamic, neurohumoral, and metabolic responses to amino acid infusion in patients with cirrhosis
- Author
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Didier Lebrec, Kahlil Nahoul, Richard Moreau, Philippe Sogni, Olivier Soubrane, Christophe Gaudin, Han Chieh Lin, Antoine Hadengue, and Eric Pussard
- Subjects
Liver Cirrhosis ,Male ,medicine.medical_specialty ,Cirrhosis ,medicine.medical_treatment ,Hemodynamics ,urologic and male genital diseases ,Kidney ,Oxygen Consumption ,Internal medicine ,medicine ,Humans ,Mannitol ,Amino Acids ,chemistry.chemical_classification ,Chemotherapy ,Hepatology ,business.industry ,Gastroenterology ,Metabolism ,Middle Aged ,medicine.disease ,Amino acid ,Endocrinology ,medicine.anatomical_structure ,chemistry ,Renal blood flow ,Vascular resistance ,Female ,business ,Atrial Natriuretic Factor ,Hormone - Abstract
In patients with cirrhosis the renal response to amino acid infusion is controversial. In addition, the renal and systemic metabolic effects of amino acids are unknown. Therefore, the present study examined the effects of amino acids on renal hemodynamics, renal and systemic oxygen (O2) consumption, and hormones in patients with cirrhosis. Twelve patients received an 8% amino acid solution for 30 minutes at a rate providing 250 mg of amino acids/kg body wt. Renal blood flow increased by 45% (P less than 0.05) and the glomerular filtration rate by only 9% (P greater than 0.05). Renal vascular resistance decreased by 23% (P less than 0.05), and renal perfusion pressure did not change significantly. Renal and systemic O2 consumption and pulmonary artery plasma glucagon level significantly increased. There were no significant changes in plasma osmolality, plasma volume, and plasma atrial natriuretic peptide concentrations. In conclusion, the results show that amino acid-induced renal vasodilation caused hyperperfusion but not renal hyperfiltration in patients with cirrhosis. In addition, renal hyperemia was associated with renal and systemic hypermetabolism.
- Published
- 1992
370. Antitumoral effect of the bisphosphonate zoledronic acid against visceral metastases in an adrenocortical cancer patient
- Author
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Jérôme Alexandre, Xavier Bertagna, Olivier Soubrane, F. Goldwasser, and Pascaline Boudou-Rouquette
- Subjects
Oncology ,medicine.medical_specialty ,business.industry ,medicine.medical_treatment ,Treatment outcome ,Hematology ,Bisphosphonate ,Zoledronic acid ,Internal medicine ,medicine ,business ,Adrenocortical cancer ,medicine.drug - Published
- 2009
371. Endoscopic Therapy of Biliary Fistulas After Complex Liver Resections
- Author
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Olivier Scatton, Bourrier Anne, Stanislas Chaussade, Ariane Chryssostalis, Bertrand Dousset, Frédéric Prat, Olivier Soubrane, Pierre Philippe Massault, Said Farhat, and Marianne Gaudric
- Subjects
medicine.medical_specialty ,business.industry ,General surgery ,Gastroenterology ,Medicine ,Radiology, Nuclear Medicine and imaging ,Liver resections ,business - Published
- 2009
372. Relationships between plasma atrial natriuretic peptide concentrations and hemodynamics and hematocrit in patients with cirrhosis
- Author
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Antoine Hadengue, Eric Pussard, Richard Moreau, Olivier Soubrane, Philippe Sogni, Christophe Gaudin, and Didier Lebrec
- Subjects
Liver Cirrhosis ,medicine.medical_specialty ,Cirrhosis ,Hemodynamics ,Hematocrit ,Pulmonary Artery ,Atrial natriuretic peptide ,Internal medicine ,medicine.artery ,medicine ,Humans ,Splanchnic Circulation ,Hepatology ,medicine.diagnostic_test ,business.industry ,Osmolar Concentration ,Arteries ,medicine.disease ,Pulmonary hypertension ,Oxygen tension ,Oxygen ,Pulmonary artery ,Cardiology ,Portal hypertension ,Regression Analysis ,business ,Atrial Natriuretic Factor - Abstract
We studied the relationships in 29 patients with cirrhosis between pulmonary arterial atrial natriuretic peptide concentrations and the following: systemic and splanchnic hemodynamics, the hematocrit, arterial oxyhemoglobin saturation, oxygen tension and the severity of cirrhosis. Plasma atrial natriuretic peptide concentrations ranged from 21 to 208 pg/ml and averaged 78 ± 8 pg/ml (mean ± S.E.M.). Simple regression analysis showed significant correlations between plasma atrial natriuretic peptide concentration and the following: hematocrit, mean pulmonary arterial pressure, wedged hepatic venous pressure, free hepatic venous pressure, pulmonary wedged pressure and serum bilirubin concentrations. No significant correlations were found between plasma atrial natriuretic peptide concentrations and all other hemodynamic values, arterial oxyhemoglobin saturation and oxygen tension. Multiple stepwise regression analysis showed that the hematocrit, mean pulmonary arterial pressure and wedged hepatic venous pressure were significant and independent predictors of pulmonary artery plasma atrial natriuretic peptide concentrations (R2 = 0.69). Partial regression coefficients were −0.74 (p
- Published
- 1991
373. Early chronic administration of propranolol reduces the severity of portal hypertension and portal-systemic shunts in conscious portal vein stenosed rats
- Author
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Han-Chieh Lin, Olivier Soubrane, Didier Lebrec, and Stéphane Cailmail
- Subjects
Male ,Cardiac output ,Time Factors ,Portal venous pressure ,Hemodynamics ,Collateral Circulation ,Propranolol ,Constriction, Pathologic ,Severity of Illness Index ,Mesenteric Veins ,Hypertension, Portal ,medicine ,Animals ,Splanchnic Circulation ,Hepatology ,business.industry ,Portal Vein ,Rats, Inbred Strains ,medicine.disease ,Rats ,Stenosis ,medicine.anatomical_structure ,Anesthesia ,Portal hypertension ,Splanchnic ,business ,Artery ,medicine.drug - Abstract
We investigated the effects of early coronic administration of propranolol on systemic and splanchnic hemodynamic changes, and the development of portal-systemic shunts in conscious, unrestrained, portal vein stenosed rats. Compared to rats receiving placebo, early chronic propranolol (75 mg kg −1 day −1 ) administration to rats begun 3 days before portal vein stenosis and then continued for 10 consecutive days, resulted in a significant decrease in both portal pressure (11.8 ± 1.5 mmHg) and portal-systemic shunts (48 ± 18%) which were measured 2 to 3 h after the final dose of propranolol (15.2 ± 1.5 mmHg and 84 ± 5%, respectively). These beneficial effects were also observed 18 to 24 h after the final dose of chronic propranolol. In rats given propranolol continuously for 5 days starting 5 days after portal vein stenosis, portal pressure (11.8 ± 1.2 mmHg) was significantly lower than in the placebo group but portal-systemic shunts (76 ± 14%) were not significantly different. In rats receiving a single dose of propranolol (75 mg/kg) 10 days after portal vein stenosis and measured 2 to 3 h after propranolol administration, portal pressure (12.8 ±1.0 mmHg) was significantly lower than in the placebo group. Portal-systemic shunts (72 ± 17%), however, showed no significant difference from the placebo group. Similar values in portal pressure (13.3 ± 1.2 mmHg) and portal-systemic shunts (83 ± 21%) were also observed in rats 18 to 24 h after a single dose of propranolol. In addition, although propranolol administration did not alter hepatic artery blood flow, the fraction of cardiac output reaching the liver increased significantly after chronic propranolol administration. We also demonstrated that chronic propranolol administration started before portal vein stenosis limited the initial rise in portal pressure which follows portal vein stenosis. We concluded that early chronic propranolol administration reduces the severity of portal hypertension and portal-systemic shunts in portal vein stenosed rats. These beneficial effects are probably a result of a limited initial increase in portal pressure immediately following portal vein stenosis.
- Published
- 1991
374. Treatment of Stage IVA Hepatocellular Carcinoma—Reply
- Author
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Olivier Scatton, Mircea Chirica, and Olivier Soubrane
- Subjects
Oncology ,medicine.medical_specialty ,business.industry ,Internal medicine ,medicine ,Carcinoma ,Stage IVA Hepatocellular Carcinoma ,Surgery ,business ,medicine.disease - Published
- 2008
375. Transplantation hépatique et hépatopathie d’origine alcoolique : opinions des médecins de France
- Author
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Gwenaëlle Vidal-Trecan, Olivier Soubrane, F. Conti, Philippe Podevin, Y. Calmus, and V. Perut
- Subjects
Epidemiology ,Public Health, Environmental and Occupational Health - Published
- 2008
376. Orthotopic liver transplantation for Byler disease
- Author
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Jacques Valayer, Denis Devictor, Didier Houssin, Frédéric Gauthier, Olivier Soubrane, Olivier Bernard, and Y Chapuis
- Subjects
Male ,medicine.medical_specialty ,Adolescent ,medicine.medical_treatment ,Disease ,Cholestasis, Intrahepatic ,Liver transplantation ,Postoperative Complications ,Cholestasis ,Quality of life ,medicine ,Humans ,Child ,Retrospective Studies ,Transplantation ,business.industry ,Infant ,Retrospective cohort study ,medicine.disease ,Thrombosis ,Surgery ,Liver Transplantation ,El Niño ,Child, Preschool ,Female ,BYLER DISEASE ,business - Abstract
Byler disease is a rare form of familial intrahepatic cholestasis that is fatal before puberty. This retrospective study reviewed the results of orthotopic liver transplantation in 14 children with Byler disease using 12 whole-liver grafts and 2 reduced-size grafts. One post-operative death occurred after retransplantation for arterial thrombosis. In the other patients, infectious problems and rejection episodes were the most frequent complications during the postoperative period. In the 13 patients alive, graft function, growth, and quality of life were good after an average follow-up of 17 months without any sign of disease recurrence.
- Published
- 1990
377. Reversible tumor metastases growth acceleration following bevacizumab interruption and surgical resection of metastases
- Author
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François Goldwasser, Olivier Soubrane, Wulfran Cacheux, O. Vignaux, T. Boisserie, S. Chaussade, and Bertrand Dousset
- Subjects
Oncology ,Surgical resection ,Cancer Research ,medicine.medical_specialty ,Bevacizumab ,medicine.drug_class ,business.industry ,Colorectal cancer ,Cytotoxic chemotherapy ,Monoclonal antibody ,medicine.disease ,Internal medicine ,medicine ,business ,medicine.drug - Abstract
14119 Background: The addition of bevacizumab (BV), an anti-VEGF monoclonal antibody with antiangiogenic activity, to cytotoxic chemotherapy (CH) improves survival in metastatic colorectal cancer patients (pts). Reversal of VEGF inhibition is associated with rapid vascular regrowth in tumors in vivo (M.R. Mancuso et al, J Clin Invest; 116(10): 2610–2621, 2006). In the clinical setting, the consequences of BV interruption are poorly documented, especially in interactions with the surgical resection of liver metastases. Methods: We describe the kinetics of tumor growth prior to, during, and after interruption of BV and under BV reintroduction, in consecutive colorectal cancer pts experiencing objective response under CH+BV, followed by surgical resection of metastases. We measured either clinically, of using CT-scan, the diameter of target lesions according to RECIST criteria, and the doubling time. Results: 7 pts (3 F, 4M), with median age of 54 years (41–70), were treated with 5-FU based CH (+oxaliplatin: 5; + irinotecan: 1 pt) and BV for metastatic colo- (6 pts) rectal (1 pt) cancer received a median number of 6 (4–18) cycles. Median time between BV interruption and surgery: 8 weeks (3–12). Under BV, all pts experienced objective response, with a median tumor reduction of 13%/month (extr: 6–15%). After BV interruption, under CH alone, 2/7 pts experienced disease progression, with a tumor growth of the residual disease of +7% and +200%/month, respectively. In pts who underwent surgery, during the off therapy period, tumor growth occurred in all 7 pts with a kinetics of +17 to +400%/month. Tumor growth doubling time after BV interruption ranged between 2 and 5 weeks. Reintroduction of BV re-induced a tumor response in 5/7 pts with a median time to response of 8 weeks (6–12) and a reduction of 10%/month. Conclusions: We show reproducible evidence of tumor growth acceleration following BV interruption. Surgery might transiently modify the balance between angiogenic and anti-angiogenic factors. We suggest to reduce the duration of BV interruption and to restart BV as soon as possible after surgery. These observations justify a large prospective analysis of the variations of tumor growth in this setting. No significant financial relationships to disclose.
- Published
- 2007
378. Efficiency of the Endoscopic Therapy of Biliary Fistulas Secondary to Complex Liver Resections
- Author
-
Anne Bourrier, Ariane Chryssostalis, Marianne Gaudric, Olivier Scatton, Pierre-Philippe Massault, Olivier Soubrane, Stanislas Chaussade, and Frederic Prat
- Subjects
medicine.medical_specialty ,business.industry ,General surgery ,Gastroenterology ,medicine ,Radiology, Nuclear Medicine and imaging ,Liver resections ,business - Published
- 2007
379. CA 27-Effet de l’interleukine-4 sur la production de collagène par les fibroblastes intrahépatiques humains
- Author
-
Lynda Aoudjehane, Philippe Podevin, Sandrine Chouzenoux, Filomena Conti, Yvon Calmus, Pierre-Philippe Massault, Olivier Scatton, Olivier Soubrane, and Bertrand Dousset
- Subjects
business.industry ,Gastroenterology ,Medicine ,General Medicine ,business ,Molecular biology - Published
- 2006
380. CA46 - Mise au point d’une technique permettant d’optimiser la détection de la réplication du virus de l’hépatite C
- Author
-
Philippe Podevin, Olivier Soubrane, Philippe Sogni, Yvon Calmus, L. Grira, Matthieu Carrière, Stanislas Chaussade, M. Andrieu, P. Jaffray, A.R. Rosenberg, V. Pène, A. Breiman, Sandrine Chouzenoux, and Filomena Conti
- Subjects
business.industry ,Gastroenterology ,Medicine ,General Medicine ,business ,Molecular biology - Published
- 2005
381. CO19 - La protéine HIP/PAP, facteur mitogéne et anti-apoptotique : un traitement pour L’insuffisance hépatocellulaire ?
- Author
-
H.T. Lieu, L. Christa, A. Pauloin, Flora Zavala, Bernard Weill, Carole Nicco, J.G. Tralhao, Frédéric Batteux, M.T. Simon, A. Cortes, Olivier Soubrane, and Christian Bréchot
- Subjects
Gynecology ,medicine.medical_specialty ,business.industry ,Gastroenterology ,medicine ,General Medicine ,business - Published
- 2004
382. 1122 Severe hepatic sinusoidal obstruction associated with oxaliplatin-based chemotherapy for metastatic colorectal cancer
- Author
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Olivier Soubrane, Catherine Brezault, Philippe Morel, V. Audard, Bertrand Dousset, Laura Rubbia-Brandt, M Lecharpentier, P. Sartoretti, Roth Ad, and Stanislas Chaussade
- Subjects
Oncology ,medicine.medical_specialty ,Chemotherapy ,Hepatology ,Colorectal cancer ,business.industry ,medicine.medical_treatment ,Internal medicine ,medicine ,medicine.disease ,business ,Oxaliplatin ,medicine.drug - Published
- 2003
383. PITFALLS OF DOMINO TRANSPLANT
- Author
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Pierre-Philippe Massault, Didier Houssin, Moussa Matmar, João Eduardo Nicoluzzi, Olivier Soubrane, Yvon Calmus, and Bertrand Dousset
- Subjects
Transplantation ,medicine.medical_specialty ,business.industry ,medicine ,Intensive care medicine ,business ,Domino - Published
- 2001
384. Is acute rejection deleterious to long-term liver allograft function?
- Author
-
Filomena Conti, Didier Houssin, Brigitte Cherruau, Albert Louvel, Olivier Soubrane, Yvon Calmus, and Bertrand Dousset
- Subjects
Adult ,Graft Rejection ,Male ,medicine.medical_specialty ,Adolescent ,medicine.medical_treatment ,Liver transplantation ,Gastroenterology ,chemistry.chemical_compound ,Immunopathology ,Internal medicine ,medicine ,Humans ,Transplantation, Homologous ,Prospective Studies ,Prospective cohort study ,Immunosuppression Therapy ,medicine.diagnostic_test ,Hepatology ,business.industry ,Immunosuppression ,Middle Aged ,Hepatitis C ,Surgery ,Liver Transplantation ,Term (time) ,Transplantation ,chemistry ,Liver ,Cardiology ,Female ,Complication ,Liver function tests ,business ,Indocyanine green ,Function (biology) - Abstract
The decreasing incidence of chronic rejection after liver transplantation emphasizes the need for an alternative end-point to assess the long-term consequences of acute rejection. The purpose of this study was to determine the effects of resolved episodes of acute rejection on late liver allograft function.Parameters of hepatic function (liver biochemistry, indocyanine green and sulfobromophthalein clearances, histology) were analyzed in 170 consecutive adult recipients, who were followed prospectively on the basis of repeat annual work-up. Mean follow-up was 3.7+/-0.2 years.The rates of acute and chronic rejection were 51% and 4.1%, respectively. At the last follow-up, there was no significant difference in graft function between patients with a single episode of acute rejection (n=56) and those without rejection (n=84). Among patients treated for a single episode of acute rejection, late hepatic function was not influenced by the severity of acute rejection and the response to corticosteroids. In contrast, patients with recurrent acute rejection (n=30) had significant impairment of liver function tests (aspartate aminotransferase, p0.05; alanine aminotransferase, p0.01; alkaline phosphatase, p0.01; gamma-glutamyl transpeptidase, p0.001), lower dye clearances (indocyanine green, p0.01; sulfobromophthalein, p0.01) and more severe histologic damage (p0.001).Single episodes of acute rejection do not impair the long-term hepatic function, whereas recurrent episodes leave sequellar damage to the liver allograft. These results provide a rationale for converting patients with rejection to a heavier immunosuppressive regimen, while leaving nearly half the recipients on a lifelong light immunosuppressive regimen.
- Published
- 1998
385. R093 Place de l'apoptose au cours de l'ischemie-reperfusion hepatique: Interet d'une strategie anti-apoptotique
- Author
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Alexandre Mignon, Anne Wernet, Olivier Soubrane, JF Dhainaut, A Kahn, A de la Coste, Monique Fabre, and J.L. De Godoy
- Subjects
Anesthesiology and Pain Medicine ,General Medicine - Published
- 1998
386. R483 Résection caecale et lavage dans un modèle murin de choc septique: modulation de la réponse inflammatoire
- Author
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JF Dhainaut, Alexandre Mignon, Olivier Soubrane, J.L. De Godoy, and B Bouffandeau
- Subjects
Anesthesiology and Pain Medicine ,General Medicine - Published
- 1998
387. Des médicaments pour traiter les hépatites fulminantes?
- Author
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Alexandre Mignon, A Kahn, Christine Perret, A La Coste, JF Dhainaut, Olivier Soubrane, and Monique Fabre
- Subjects
Anesthesiology and Pain Medicine ,General Medicine - Published
- 1997
388. Intraoperative Blood Loss in Pediatric Liver Transplantation
- Author
-
YVES M. OZIER, BRIGITTE LE CAM, GILLES CHATELLIER, DANIEL EYRAUD, OLIVIER SOUBRANE, DIDIER HOUSSIN, CHRISTIAN CONSEILLER, and Charlotte Bell
- Published
- 1996
389. Recurrent liver abscess secondary to ingested fish bone migration: Report of a case.
- Author
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Frédéric Clarençon, Olivier Scatton, Eric Bruguière, Stéphane Silvera, Guillaume Afanou, Olivier Soubrane, Olivier Vignaux, and Paul Legmann
- Subjects
ABSCESSES ,LIVER abscesses ,ABDOMINAL pain ,BILIOUS diseases & biliousness ,GASTROINTESTINAL diseases - Abstract
Abstract  Pyogenic liver abscess is an unusual cause of fever and abdominal pain, but it is potentially fatal. It is rarely caused by a local event, but rather by hematogenous dissemination or biliary tract disease. We report an uncommon case of liver abscess caused by the migration of a fish bone through the gastrointestinal wall. [ABSTRACT FROM AUTHOR]
- Published
- 2008
390. A novel, sensitive, and specific RT‐PCR technique for quantitation of hepatitis C virus replication.
- Author
-
Matthieu Carrière, Véronique Pène, Adrien Breiman, Filoména Conti, Sandrine Chouzenoux, Eliane Meurs, Muriel Andrieu, Patrick Jaffray, Lilia Grira, Olivier Soubrane, Philippe Sogni, Yvon Calmus, Stanislas Chaussade, Arielle R. Rosenberg, and Philippe Podevin
- Subjects
HEPATITIS C ,VIRAL hepatitis ,VIRAL replication ,LIVER diseases - Abstract
The detection of negative‐strand hepatitis C virus (HCV) RNA is a hallmark of replication. A highly sensitive and specific method is required to quantify the very low level of replication inherent to in vitro infection systems. Based on reverse transcription with a tagged primer in the 5′ non‐coding region of the HCV genome, followed by a nested PCR with a second round of real‐time PCR, a novel method is described with improved sensitivity for negative‐strand HCV RNA quantification. The lower detection level was 25 copies per reaction of negative‐strand HCV RNA, even in the presence of 1 × 105 copies of positive‐strand HCV RNA. This protocol was applied to the detection of negative HCV strand RNA in the liver of HCV‐infected patients as well as in primary human hepatocytes infected in vitro. In both models, and particularly in each of three, independent in vitro infection experiments, this assay permitted the quantitation of HCV replication. J. Med. Virol. 79:155–160, 2007. © 2006 Wiley‐Liss, Inc. [ABSTRACT FROM AUTHOR]
- Published
- 2007
391. Laparoscopic Left Lateral Sectionectomy in Living Donors: Safety and Reproducibility of the Technique in a Single Center.
- Author
-
Olivier Soubrane
- Abstract
BACKGROUND DATA AND OBJECTIVE:: Left lateral sectionectomy for liver transplantation in children performed through laparoscopy is an innovative procedure that was developed by considering our acquired experience in both laparoscopic liver resection and graft harvesting in living donors. The main goal was to minimize donor morbidity while preserving the abdominal wall. Herein, we report the technical feasibility and reproducibility, and compared it with open liver resection (OLR).METHODS:: Sixteen successive donors underwent a laparoscopic liver resection (LLR) from 2001 to 2005. They were compared with 14 other donors who underwent a standard open liver resection (OLR) during a first period (1998–2004). First, this report describes the technical features of laparoscopic resection. Second, perioperative morbidity and graft characteristics were compared according to the use or not of the laparoscopic approach.RESULTS:: Laparoscopic harvesting was successfully performed in 15 of 16 cases in an intention-to-treat basis. One conversion was required to ensure the quality of the laparoscopic repair of a left portal vein injury occurring during the pedicle dissection. No specific complication related to laparoscopy was observed. As compared with OLR, the operation was longer (320 ± 67 vs. 244 ± 55 minutes, P < 0.005). The blood loss was significantly lower in the LLR group (18.7 ± 44.2 vs. 199.2 ± 185.4 mL, P < 0.005). The morbidity rate was similar in both groups (18.7% in LLR vs. 35.7% in OLR). One donor in the LLR group experienced a bile leak treated by redo laparoscopy. Grafts were anatomically similar irrespective of the use of laparoscopy. The duration of hospital stay and use of self-infused morphine pump was not different between the 2 groups.CONCLUSION:: Left lateral section harvesting by laparoscopy is a safe and reproducible procedure, allowing to obtain similar grafts as compared with laparotomy and can therefore be recommended to transplant centers that have previous experience in laparoscopic liver resection. [ABSTRACT FROM AUTHOR]
- Published
- 2006
- Full Text
- View/download PDF
392. Relationships between plasma atrial natriuretic peptide (ANP) and hematocrit, pulmonary artery and portal pressures in patients with cirrhosis
- Author
-
Didier Lebrec, Olivier Soubrane, Christophe Gaudin, Antoine Hadengue, Eric Pussard, Richard Moreau, and Philippe Sogni
- Subjects
medicine.medical_specialty ,Cirrhosis ,Hepatology ,medicine.diagnostic_test ,business.industry ,Hematocrit ,medicine.disease ,Atrial natriuretic peptide ,Internal medicine ,medicine.artery ,Pulmonary artery ,medicine ,Cardiology ,In patient ,business - Published
- 1991
393. Amino acids (AA) infusion induces renal hyperemia but not hyperfiltration in patients with cirrhosis
- Author
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Richard Moreau, Olivier Soubrane, Christophe Gaudin, Philippe Sogni, Didier Lebrec, Antoine Hadengue, and Eric Pussard
- Subjects
chemistry.chemical_classification ,medicine.medical_specialty ,Cirrhosis ,Endocrinology ,Hepatology ,chemistry ,business.industry ,Internal medicine ,Medicine ,In patient ,business ,medicine.disease ,Amino acid - Published
- 1991
394. Renal oxygen consumption in patients with cirrhosis
- Author
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Antoine Hadengue, Olivier Soubrane, Christophe Gaudin, Didier Lebrec, Richard Moreau, and Philippe Sogni
- Subjects
Consumption (economics) ,medicine.medical_specialty ,Cirrhosis ,Hepatology ,business.industry ,chemistry.chemical_element ,medicine.disease ,Gastroenterology ,Oxygen ,chemistry ,Internal medicine ,Medicine ,In patient ,business - Published
- 1991
395. Pancreatic fistula after pancreaticoduodenectomy: the conservative treatment of choice
- Author
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Olivier Scatton, Wellington Andraus, Olivier Soubrane, Bruto Randone, Pierre-Philippe Massault, Luciana Bertocco de Paiva Haddad, and Bertrand Dousset
- Subjects
Pancreatic duct ,medicine.medical_specialty ,Adenoma ,Hepatology ,pancreatic resection ,business.industry ,medicine.medical_treatment ,Incidence (epidemiology) ,Gastroenterology ,Original Articles ,medicine.disease ,Pancreaticoduodenectomy ,Surgery ,medicine.anatomical_structure ,pancreatic fistula ,Pancreatic fistula ,Pancreatectomy ,Carcinoma ,postoperative complications ,Medicine ,pancreaticoduodenectomy ,business ,Complication - Abstract
BackgroundA pancreatic fistula (PF) is the most common complication after pancreaticoduodenectomy (PD), and its reported incidence varies from 2% to 28%. The aim of the present study was to analyse the treatment of a complicated PF comparing the surgical approach with conservative techniques.MethodsFrom January 2000 through to August 2006, 121 patients were submitted for PD. The study consisted of 70 men and 47 women, with a median age of 60 years (SD ± 12). The main indications for PD were pancreatic duct carcinoma in 52 patients (44.5%), ampullary carcinoma or adenoma in 18 (15.4%) and islet cell tumour in 11 (9.4%). Reconstruction by pancreatogastrostomy was performed in 65 patients (55.6%), and pancreatojejunostomy in 52 patients (44%).ResultsThirty-five patients (30%) developed a PF. Amongst these, 20 were managed conservatively and 14 were reoperated. These two groups of patients were compared with patients without a PF for analysis. There was no significant difference in the mean age, the gender ratio, American Society of Anesthesiologists (ASA) classification, surgical time and blood replacement, number of associated procedures, vascular resection and type of reconstruction between the three groups. There were five post-operative deaths (4.2%), three patients (21.4%) in the surgical treatment group (P < 0.01). Mean total number of complications (P= 0.02) and mean length of hospital stay (P < 0.001) were greater in the surgical group. The medium delay between the pancreatic resection and reoperation was 10 days (range, 3–32 days). Completion splenopancreatectomy was required in five patients whereas conservative treatment including debridement and drainage was applied in nine patients.ConclusionThe surgical approach for a PF is associated with a higher mortality and morbidity. There is no advantage in performing completion pancreatectomy (CP) instead of extensive drainage as a result of the same mortality and morbidity rates and the risk of endocrine insufficiency. In cases of complicated PF, radiological or surgical conservative treatment is recommended.
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396. Adjuvant therapies in advanced hepatocellular carcinoma: moving forward from the STORM
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Sandrine Faivre, Mohamed Bouattour, Armand de Gramont, and Olivier Soubrane
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Niacinamide ,0301 basic medicine ,Sorafenib ,Oncology ,medicine.medical_specialty ,Pathology ,Letter ,Carcinoma, Hepatocellular ,Time Factors ,Local ablation ,medicine.medical_treatment ,Medicine (miscellaneous) ,Angiogenesis Inhibitors ,Disease-Free Survival ,03 medical and health sciences ,Clinical trials ,0302 clinical medicine ,Internal medicine ,medicine ,Hepatectomy ,Humans ,Pharmacology (medical) ,Randomized Controlled Trials as Topic ,Antiangiogenic agents ,business.industry ,Phenylurea Compounds ,Liver Neoplasms ,medicine.disease ,Clinical Practice ,Clinical trial ,Treatment Outcome ,030104 developmental biology ,Chemotherapy, Adjuvant ,030220 oncology & carcinogenesis ,Hepatocellular carcinoma ,Catheter Ablation ,Disease Progression ,Neoplasm Recurrence, Local ,business ,Adjuvant ,Antiangiogenic drug ,medicine.drug - Abstract
Like other previous treatments and approaches, sorafenib, an antiangiogenic drug, failed to show any benefit in the adjuvant setting for hepatocellular carcinoma in a large clinical trial. We discuss reasons and implications of these negative results and the implications for clinical practice and future research. ClinicalTrials.gov: NCT00692770 . Registered 5 June 2008. This study has been completed.
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397. Locoregional treatments before liver transplantation for hepatocellular carcinoma: A cohort study from the European Liver Transplant Registry
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Hans-Christian, Pommergaard, Andreas Arendtsen Rostved, René, Adam, Lau Caspar Thygesen, Mauro, Salizzoni, Miguel Angel Gómez Bravo, Daniel, Cherqui, DE SIMONE, Paolo, Karim, Boudjema, Vincenzo, Mazzaferro, Olivier, Soubrane, Juan Carlos García-Valdecasas, Joan Fabregat Prous, Pinna, Antonio D., John, O’Grady, Vincent, Karam, Christophe, Duvoux, and Allan, Rasmussen
398. Interest of preoperative immunonutrition in liver resection for cancer: study protocol of the PROPILS trial, a multicenter randomized controlled phase IV trial
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Olivier Scatton, Astrid Schielke, Gabriella Pittau, O. Ciacio, Perrine Goyer, Stéphane Benoist, Emmanuel Boleslawski, Jean-Christophe Vaillant, Denis Castaing, Daniel Azoulay, Eric Vibert, Hélène Agostini, René Adam, Maïté Lewin, Chady Salloum, Thibault Voron, Olivier Soubrane, Antonio Sa Cunha, Didier Samuel, Laurent Hannoun, and Daniel Cherqui
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Sarcopenia ,medicine.medical_specialty ,Cancer Research ,medicine.medical_treatment ,Nutritional Status ,Chronic liver disease ,Preoperative care ,law.invention ,Immunonutrition ,Cholangiocarcinoma ,Eating ,Study Protocol ,Liver metastases ,Enteral Nutrition ,Double-Blind Method ,Randomized controlled trial ,law ,Preoperative Care ,medicine ,Genetics ,Hepatectomy ,Humans ,Surgical Wound Infection ,Prospective Studies ,HCC ,Intention-to-treat analysis ,Liver resection ,business.industry ,Liver Neoplasms ,Perioperative ,Length of Stay ,medicine.disease ,Anti-Bacterial Agents ,Intention to Treat Analysis ,Liver Regeneration ,Surgery ,Clinical trial ,Parenteral nutrition ,Oncology ,Research Design ,Dietary Supplements ,Patient Compliance ,business ,Immunocompetence - Abstract
Malnutrition is an independent risk factor of postoperative morbidity and mortality and it’s observed in 20 to 50% of surgical patients. Preoperative interventions to optimize the nutritional status, reduce postoperative complications and enteral nutrition has proven to be superior to the parenteral one. Moreover, regardless of the nutritional status of the patient, surgery impairs the immunological response, thus increasing the risk of postoperative sepsis. Immunonutrition has been developed to improve the immunometabolic host response in perioperative period and it has been proven to reduce significantly postoperative infectious complications and length of hospital stay in patients undergoing elective gastrointestinal surgery for tumors. We hypothesize that a preoperative oral immunonutrition (ORAL IMPACT®) can reduce postoperative morbidity in liver resection for cancer. Prospective multicenter randomized placebo-controlled double-blind phase IV trial with two parallel treatment groups receiving either study product (ORAL IMPACT®) or control supplement (isocaloric isonitrogenous supplement - IMPACT CONTROL®) for 7 days before liver resection for cancer. A total of 400 patients will be enrolled. Patients will be stratified according to the type of hepatectomy, the presence of chronic liver disease and the investigator center. The main end-point is to evaluate in intention-to-treat analysis the overall 30-day morbidity. Secondary end-points are to assess the 30-day infectious and non-infectious morbidity, length of antibiotic treatment and hospital stay, modifications on total food intake, compliance to treatment, side-effects of immunonutrition, impact on liver regeneration and sarcopenia, and to perform a medico-economic analysis. The overall morbidity rate after liver resection is 22% to 42%. Infectious post-operative complications (12% to 23%) increase the length of hospital stay and costs and are responsible for a quarter of 30-day mortality. Various methods have been advocated to decrease the rate of postoperative complications but there is no evidence to support or refute the use of any treatment and further trials are required. The effects of preoperative oral immunonutrition in non-cirrhotic patients undergoing liver resection for cancer are unknown. The present trial is designed to evaluate whether the administration of a short-term preoperative oral immunonutrition can reduce postoperative morbidity in non-cirrhotic patients undergoing liver resection for cancer. Clinicaltrial.gov: NCT02041871 .
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399. 168* Combined lung and liver transplantation in cystic fibrosis
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R. Kanaan, D. Hubert, Daniel Dusser, Jean-Noël Fabiani, V. Boussaud, Romain Guillemain, Olivier Soubrane, Isabelle Sermet, Redha Souilamas, and Gérard Lenoir
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Pulmonary and Respiratory Medicine ,Pathology ,medicine.medical_specialty ,Lung ,business.industry ,medicine.medical_treatment ,Liver transplantation ,medicine.disease ,Cystic fibrosis ,medicine.anatomical_structure ,Pediatrics, Perinatology and Child Health ,medicine ,Pediatrics, Perinatology, and Child Health ,business - Full Text
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400. Laparoscopic liver resection for living donation: where do we stand?
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Cauchy F, Schwarz L, Scatton O, and Soubrane O
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- Age Factors, Hepatectomy adverse effects, Humans, Laparoscopy adverse effects, Liver Transplantation adverse effects, Risk Assessment, Risk Factors, Treatment Outcome, Hepatectomy methods, Laparoscopy methods, Liver Transplantation methods, Living Donors
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In Western countries, living donor liver transplantation (LDLT) may represent a valuable alternative to deceased donor liver transplantation. Yet, after an initial peak of enthusiasm, reports of high rates of complications and of fatalities have led to a certain degree of reluctance towards this procedure especially in Western countries. As for living donor kidney transplantation, the laparoscopic approach could improve patient's tolerance in order to rehabilitate this strategy and reverse the current trend. In this setting however, initial concerns regarding patient's safety and graft integrity, need for acquiring surgical expertise in both laparoscopic liver surgery and living donor transplantation and lack of evidence supporting the benefits of laparoscopy have delayed the development of this approach. Similarly to what is performed in classical resectional liver surgery, initial experiences of laparoscopy have therefore begun with left lateral sectionectomy, which is performed for adult to child living donation. In this setting, the laparoscopic technique is now well standardized, is associated with decreased donor blood loss and hospital stays and provides graft of similar quality compared to the open approach. On the other hand laparoscopic major right or left hepatectomies for adult-adult LDLT currently lack standardization and various techniques such as the full laparoscopic approach, the hand assisted approach and the hybrid approach have been reported. Hence, even-though several reports highlight the feasibility of these procedures, the true benefits of laparoscopy over laparotomy remain to be fully assessed. This could be achieved through standardization of the procedures and creation of international registries especially in Eastern countries where LDLT keeps on flourishing.
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- 2014
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