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Introduction: Palatal expansion has been a popular and proven technique for transverse discrepancies used in orthodontics for decades. The short-term effectiveness of the technique is understood, yet questions remain regarding the long-term stability, with much debate surrounding the optimum treatment timing to initiate expansion for the most beneficial and stable results, especially with regard to mixed dentition treatment. The purpose of this study was to determine the long-term stability of palatal expansion performed in the mixed dentition vs the permanent dentition., Methods: Fifty-four subjects were grouped as follows: 24 in the mixed dentition and 30 in the permanent dentition at the start of treatment. All patients had been treated with the Haas type of palatal expansion appliance followed by nonextraction fixed edgewise mechanics. Digitized dental casts were evaluated at 3 times: before treatment (T1), after treatment (T2), and at the long-term retention (T3). Intermolar widths were computed by subtracting measurements between time points, and comparisons between the groups were made to determine expansion and stability differences., Results: Significant intermolar width increases were observed from T1 to T2 with significant relapses from T2 to T3, and an overall net gain remained at T3 in each group. No significant differences were found for relapse between the mixed and permanent dentitions., Conclusions: There are no differences for the long-term intermolar width stability in patients treated with palatal expansion in the mixed dentition vs the permanent dentition., (Copyright © 2016 American Association of Orthodontists. Published by Elsevier Inc. All rights reserved.)

Introduction: Esthetic improvement is a primary reason that people seek orthodontic treatment. The maxillary canine is considered by many to have great importance for both function and esthetics. Limited information is available about the position of the maxillary canine in relation to skeletal landmarks and whether the position can influence esthetic perceptions. The purposes of this study were to evaluate the normal maxillary canine position in relation to skeletal landmarks, to determine posttreatment 3-dimensional maxillary canine position with cone-beam computed tomography images, and to see whether maxillary canine position influences esthetic perceptions., Methods: The Bolton standard template was used as the control sample, and the maxillary canine position was determined by implementing a Cartesian coordinate system. The right and left maxillary canines of 96 treated patients (48 boys, 48 girls; 15 years old) were analyzed by digitization of the cone-beam computed tomography volumes. The subjects' posttreatment smile photographs were ranked and quantified by 9 orthodontic residents who completed a Q-sort. Correlations were determined between canine positions and esthetic outcomes., Results: The only difference between the right and left canine positions was the anteroposterior position of the root apex. Statistically significant sex differences were found for the superoinferior position of the right and left canine cusp tips, the mediolateral right and left canine root apices, and the mediolateral left canine cusp tips. No correlation was determined between the maxillary canine position and the esthetic perception., Conclusions: The maxillary canine position in relation to skeletal landmarks was determined and does not appear to significantly impact the esthetic perception, according to this study., (Copyright © 2016 American Association of Orthodontists. Published by Elsevier Inc. All rights reserved.)

Background: The aim of this study was to better understand how surgical repositioning and stabilization of anteriorly displaced articular discs using the Mitek mini-anchor technique affects condylar growth in growing patients with adolescent internal condylar resorption (AICR)., Methods: Twenty-two adolescent patients diagnosed with AICR and anterior temporomandibular disc displacement were compared to untreated control subjects without AICR matched for age, sex, and Angle classification. Pre-surgical (T1 and T2) and post-surgical (T3 and T4) mandibular tracings were superimposed on natural stable structures to evaluate the horizontal, vertical, and total changes in the position of condylion., Results: The treated group showed an overall decrease in condylar height pre-surgically and statistically significant changes in condylar growth direction between the pre- and post-surgical observation periods. Pre-surgically, the treated group showed significantly more posterior condylar growth than the control group; they also showed inferior condylar growth, while the controls showed superior growth. Controls and patients in the treated group showed no significant differences in condylar growth post-surgically., Conclusions: Adolescent patients diagnosed with AICR and anterior disc displacement treated with mandibular ramus and maxillary osteotomies, along with Mitek anchors to reposition internally deranged discs, showed post-surgical normalization of condylar growth.

Introduction: Long-term soft tissue response to extraction orthodontic treatment has been a subject of interest for years. The purposes of this study were to investigate long-term soft tissue profile changes in an extraction sample and to compare them with profile changes in an untreated sample., Methods: A premolar extraction-treated sample (n = 47) and an untreated control sample (n = 57) were studied. Descriptive statistics were collected, and individual t tests were used for comparison and contrast of the treated and untreated samples., Results: We found that the untreated soft tissue profile changed in the downward and forward direction. The treated soft tissue profile change was similar, but with more of a forward component than in the untreated sample. Most noteworthy was the finding that the soft tissue profiles of both the untreated and the treated samples were similar at the end point., Conclusions: The following conclusions were derived from the study. (1) There was no substantive difference in the soft tissue profiles of the samples, but there were some differences in the directional changes between them. (2) The changes for the untreated sample were the greatest for the lips and the chin, with the change occurring in the downward and forward direction. (3) The soft tissue profiles of the extraction sample also had the greatest measurable changes in the lips and the chin, but the changes had more of a forward component than they did in the untreated sample. (4) Extraction treatment does not adversely impact soft tissue profile changes over time., (Copyright © 2015 American Association of Orthodontists. Published by Elsevier Inc. All rights reserved.)

Objective: To describe maxillary growth and maturation during infancy and early childhood., Materials and Methods: Serial cephalograms (N=210) of 30 subjects (15 females and 15 males) from the Bolton-Brush Growth Study were analyzed. Each subject had a series of six consecutive cephalograms taken between birth and 5 years of age, as well as one adult cephalogram. Twelve maxillary measurements (eight linear and four angular) and seven landmarks were used to characterize maxillary growth. Maturation of the linear measures was described as a percentage of adult status., Results: Maxillary and anterior cranial base size increased in both sexes between 0.4 and 5 years of age. The linear anteroposterior (AP) measures (S-SE, SE-N, ANS-PNS) increased almost as much as the vertical measures (S-PNS, SE-PNS, N-A, N-ANS) over the first 5 years. After 5 years of age there was significantly more vertical than AP growth. The size and shape changes that occurred were greatest between 0.4 and 1 years; yearly velocities decelerated regularly thereafter. Overall linear growth changes that occurred between 0.5 and 5 years of age (a span of 4.5 years) were generally greater than the changes in maxillary growth that occurred between 5 and 16 years (a span of 11 years). The linear measures showed a gradient of maturation, with the AP measures being more mature than the vertical measures. Male maxillae were less mature than female maxillae at every age., Conclusions: The maxilla undergoes its greatest postnatal growth change during infancy and early childhood, when relative AP growth and maturation are emphasized.

Problem: How does pilot hole size and bone density affect the primary stability of miniscrew implants (MSIs)?, Methods: Using 120 MSIs divided equally into six groups, this 2 × 3 factorial design evaluated the effects of synthetic bone density (0.64 g/cc vs 0.8 g/cc cortices) and pilot hole size (no pilot hole, 1.0 mm pilot hole, and 1.4 mm pilot hole) on maximum insertion torque and pullout strength. The maximum placement torque was measured as the last thread of the MSIs was inserted. The pullout strength test applied a vertical force at 10 mm/min until failure., Results: The insertion torque and pullout strength values were significantly (p ≤ .05) greater for the MSIs placed in high-density than in low-density cortical bone. The insertion torque and pullout strength decreased as pilot hole size increased, with significant (p ≤ .05) differences between all three subgroups. Insertion torque and pullout strength were significantly intercorrelated for all subgroupings, with stronger correlations in denser bone having smaller or no pilot holes., Conclusion: Depending on bone density, pilot holes of limited size can be used to optimize primary stability by decreasing insertion torque while maintaining the pullout strength of bone., (© 2010 Wiley Periodicals, Inc.)

The two major components of the Eubacteria Sec-dependent protein translocation system are the heterotrimeric channel-forming component SecYEG and its binding partner, the SecA ATPase nanomotor. Once bound to SecYEG, the preprotein substrate, and ATP, SecA undergoes ATP-hydrolytic cycles that drive the stepwise translocation of proteins. Although a previous site-directed in vivo photocross-linking study (Mori, H., and Ito, K. (2006) Proc. Natl. Acad. Sci. U.S.A. 103, 16159-16164) elucidated residues of SecY needed for interaction with SecA, no reciprocal study for SecA protein has been reported to date. In the present study we mapped residues of SecA that interact with SecY or SecG utilizing this approach. Our results show that distinct domains of SecA on two halves of the molecule interact with two corresponding SecY partners as well as with the central cytoplasmic domain of SecG. Our data support the in vivo relevance of the Thermotoga maritima SecA·SecYEG crystal structure that visualized SecYEG interaction for only one-half of SecA as well as previous studies indicating that SecA normally binds two molecules of SecYEG.

Introduction: We compared the stability of 3- and 6-mm long miniscrew implants (MSIs) loaded with orthopedic force levels., Methods: Using a split-mouth experimental design, we placed MSIs into the jaws of 5 mature beagle dogs and immediately loaded them for 6 weeks. Continuous forces were applied by reciprocally loading pairs of MSIs with nickel-titanium coil springs. The mandibles had the 3-mm MSIs randomly loaded with forces of 600 or 900 g. In the maxilla, the 3- and 6-mm MSIs were randomly assigned and loaded with 600 g of force. An unloaded, control MSI was placed in each quadrant. Overall success was defined as MSIs that remained intact; net success rates excluded MSIs that had sheared off and all implants placed in a dog that frequently chewed his run bars and food bowl., Results: The overall success rates of the loaded and the control 6-mm MSIs were 100%. Overall and net success rates for the 3-mm experimental MSIs were 66.7% and 95.2%, respectively. Similarly, the overall and net success rates of the 3-mm control MSIs were 66.7% and 81.8%, respectively. The overall success rates of the 3-mm mandibular MSIs loaded with 900 and 600 g of force were both 60%; their net success rates were 100% with 900 g and 85.7% with 600 g. Overall success rates of the 3-mm experimental MSIs in the maxilla and the mandible were 80% and 60%, repectively. The net success rates were 100% (maxilla) and 85.7% (mandible). There were no significant (P > 0.05) differences in stability associated with force or location. The loaded 3- and 6-mm MSI pairs demonstrated significant decreases in interimplant distance, averaging 2.2 and 1.8 mm, respectively; the 3-mm MSIs loaded with 900 g showed significantly more displacement than those loaded with 600 g., Conclusions: Success rates of immediately loaded 3-mm MSIs were significantly lower than those of immediately loaded 6-mm MSIs. Neither force nor location explained differences in the success rates. The linear displacements of the MSIs were associated with load amount rather than implant length.

Introduction: The purpose of this study was to evaluate the short-term changes in posterior occlusion produced by perfector/spring aligner and Hawley retainers., Methods: In this prospective clinical study, we randomly assigned 50 patients who had completed full orthodontic treatment to a Hawley retainer group or a perfector/spring aligner retainer group. Objective and subjective measures were collected when the retainers were delivered (T1) and approximately 2 months later (T2). Blu Mousse (Parkell Bio-Materials, Farmingdale, NY) was used to quantify posterior areas of contact (<50 microm) and near contact (50-350 microm). The patient's perception of occlusion was assessed by using a 7-item questionnaire., Results: Areas of contact and near contact (ACNC) in the Hawley group increased significantly (P <0.05) from 6.71 mm(2) at T1 to 10.97 mm(2) at T2; ACNC in the perfector/spring aligner group increased from 8.44 mm(2) at T1 to 12.95 mm(2) at T2. There were no significant (P <0.05) differences in the increases of ACNC between the Hawley group (4.50 mm(2)) and the perfector/spring aligner group (3.26 mm(2)). Spearman correlations showed that ACNC at T1 were positively related with ACNC at T2 and negatively related with T2 to T1 ACNC changes. In comparison with the Hawley group, the patients wearing perfector/spring aligner retainers reported significantly greater improvements in how well their back teeth fit together, how well they could chew tough meats, and how much pain they felt when they bit down., Conclusions: ACNC increased substantially and similarly in patients wearing Hawley and perfector/spring aligner retainers. Changes over time were perceived to a greater extent by patients wearing perfector/spring aligner retainers.

SecA facilitates protein transport across the eubacterial plasma membrane by its association with cargo proteins and the SecYEG translocon, followed by ATP-driven conformational changes that promote protein translocation in a stepwise manner. Whether SecA functions as a monomer or a dimer during this process has been the subject of considerable controversy. Here we utilize cysteine-directed mutagenesis along with the crystal structure of the SecA dimer to create a cross-linked dimer at its subunit interface, which was normally active for in vitro protein translocation.

SecA is an essential ATP-driven motor protein that binds to presecretory or membrane proteins and the translocon and promotes the translocation or membrane integration of these proteins. secA is subject to a protein secretion-specific form of regulation, whereby its translation is elevated during secretion-limiting conditions. A novel mechanism that promotes this regulation involves translational pausing within the gene upstream of secA, secM. The secM translational pause prevents formation of an RNA helix that normally blocks secA translational initiation. The duration of this pause is controlled by the rate of secretion of nascent SecM, which in turn depends on its signal peptide and a functional translocon. We characterized the atypical secM signal peptide and found that mutations within the amino-terminal region specifically affect the secM translational pause and secA regulation, while mutations in the hydrophobic core region affect SecM secretion as well as translational pausing and secA regulation. In addition, mutational analysis of the 3' end of secM allowed us to identify a conserved region that is required to promote the translational pause that appears to be operative at the peptide level. Together, our results provide direct support for the secM translational pause model of secA regulation, and they pinpoint key sequences within secM that promote this important regulatory system.