232 results on '"Mulas, J."'
Search Results
202. Tamoxifen induces gonadotropin-releasing hormone self-priming through an estrogen-dependent progesterone receptor expression in the gonadotrope of the rat.
- Author
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Bellido C, Martín de las Mulas J, Tena-Sempere M, Aguilar R, Alonso R, and Sánchez-Criado JE
- Subjects
- Animals, Female, Immunohistochemistry, Luteinizing Hormone metabolism, Progesterone metabolism, Prolactin metabolism, Radioimmunoassay, Rats, Rats, Wistar, Reverse Transcriptase Polymerase Chain Reaction, Estrogens metabolism, Gonadotropin-Releasing Hormone metabolism, Pituitary Gland drug effects, Pituitary Gland metabolism, Receptors, Progesterone drug effects, Receptors, Progesterone metabolism, Selective Estrogen Receptor Modulators pharmacology, Tamoxifen pharmacology
- Abstract
Tamoxifen (TX) is an antiestrogen with varying levels of antagonist/agonist activity on the reproductive axis of the rat. It has been reported that TX, in contrast to other selective estrogen receptor modulators (SERMs), increases the content of cytosolic estrogen receptors (ER) in the gonadotrope and induces gonadotropin releasing hormone (GnRH) self-priming in the absence of E. GnRH priming is believed to be a consequence of E-dependent progesterone receptor (PR) activation. The purpose of this study was to determine whether TX induces PR expression in the gonadotrope in an E-dependent manner, and whether the blockade of PR activation affects TX-dependent GnRH self-priming in ovariectomized (OVX) rats. Chronic OVX rats were injected (sc) over 3 days with 25 microg estradiol benzoate (EB), 3 mg TX, 0.5 mg RU58668, a 'pure' anti-E (aE), 2 mg RU38486, an anti-P at the receptor (aP), TX+aE and TX+aP. Controls were given 0.2 ml oil. While EB and TX increased mRNA for both PR A+B and PR B expression and the number and intensity of nuclei immunoreactive (IR) for PR in the gonadotrope, the aE and aP given alone had no effect on either PR mRNA levels or nuclear PR-IR. The aE reduced the effect of TX on PR expression (mRNA and nuclear IR) while the aP slightly reduced nuclear PR-IR only. In addition, pituitaries from each of the seven groups were incubated with: 10(-8)M E(2), 10(-7)M TX, 10(-8)M aE, 10(-8)M aP, TX+aE, TX+aP or medium alone, respectively. Pituitaries were tested for GnRH self-priming (two pulses of 15 min 1 h apart) and the secretion of LH and PRL determined by specific RIAs. Pituitaries from rats treated with EB and incubated with E(2) had increased basal and GnRH-stimulated luteinizing hormone (LH) and prolactin (PRL) secretion and GnRH self-priming. TX reduced basal and stimulated LH secretion, increased PRL secretion and induced a robust GnRH self-priming. All these effects of TX were blocked by the aE, while the aP blocked GnRH self-priming only. In conclusion, tamoxifen induced PR expression (mRNA and nuclear IR) in the gonadotrope in an E-dependent manner, while activation of these PR through intracellular signaling of GnRH induced GnRH self-priming., (Copyright 2003 S. Karger AG, Basel)
- Published
- 2003
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203. Progesterone receptors in normal, dysplastic and tumourous feline mammary glands. Comparison with oestrogen receptors status.
- Author
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Martín de las Mulas J, Van Niel M, Millán Y, Ordás J, Blankenstein MA, Van Mil F, and Misdorp W
- Subjects
- Adenocarcinoma chemistry, Adenocarcinoma pathology, Animals, Cats, Female, Fibrocystic Breast Disease chemistry, Fibrocystic Breast Disease pathology, Male, Neoplasms chemistry, Neoplasms pathology, Sensitivity and Specificity, Immunohistochemistry methods, Mammary Neoplasms, Animal chemistry, Mammary Neoplasms, Animal pathology, Receptors, Estrogen analysis, Receptors, Progesterone analysis
- Abstract
The aims of the study were to standardise an immunohistochemical (IHC) method for the detection of progesterone receptors (PR) on formalin-fixed, paraffin wax-embedded tissue sections of feline mammary gland tumours and dysplasias, comparing the results with those obtained using the radiolabelled ligand dextran coated charcoal (DCC) assay applied to frozen tissue samples from the same cases. Also, to define the immunohistochemical distribution of PR in the different cellular compartments of the lesions and to compare the oestrogen receptor (ER) and PR status of the feline mammary lesions. Proliferative mammary lesions collected from 34 cats were studied; 25 malignant tumours and 9 benign tumours and dysplasias. PR protein was present at a concentration of 5 fmol mg(-1) (positivity threshold) in 37.5 per cent of malignant tumours and 66.7 per cent of benign tumours and dysplasias while immunoreative products to PR antibody were found in the nuclei of tumour cells in 38.5 per cent and 66.7 per cent of the cases, respectively. Concordance between DCC-PR and IHC-PR was 88.5 per cent (P<0.001). The specificity (true negatives) and sensitivity (true positives) of the IHC method were 89.4 per cent and 87.5 per cent respectively. The presence of PR was linked to the absence of ovariectomy (P<0.02). Oestrogen receptors, detected by either method, were also detected in half the cases in which PR had been detected. In malignant tumours, the most prevalent groups were the ER + PR + and ER-PR + groups., (Copyright 2002 Elsevier Science Ltd. All rights reserved.)
- Published
- 2002
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204. Pathological and immunohistochemical study of the abomasum and abomasal lymph nodes in goats experimentally infected with Haemonchus contortus.
- Author
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Pérez J, Garcia PM, Hernandez S, Martinez-Moreno A, Martin de las Mulas J, and Camara S
- Subjects
- Abomasum immunology, Abomasum parasitology, Animals, Antibodies, Protozoan blood, B-Lymphocytes pathology, Cell Count veterinary, Gastric Mucosa immunology, Gastric Mucosa pathology, Goat Diseases immunology, Goat Diseases parasitology, Goats, Haemonchiasis immunology, Haemonchiasis pathology, Haemonchus immunology, Immunoglobulin G analysis, Immunohistochemistry veterinary, Lymph Nodes immunology, Lymph Nodes parasitology, Male, T-Lymphocytes pathology, Abomasum pathology, Goat Diseases pathology, Haemonchiasis veterinary, Lymph Nodes pathology
- Abstract
Histopathological changes and the distributions of T and B lymphocytes and IgG producing plasma cells were recorded in the abomasum and abomasal lymph nodes of goats 3, 7 and 21 weeks post-infection (wpi) after an experimental infection with H. contortus. The low rate of worm recovery by 3 wpi (5.6%) might have been due to larvae death as suggested by the presence of granulomas in the abomasal mucosa at 3 and 7 wpi, or simply due to a poor larval establishment. Marked increase in the secretion of mucus by mucous cells together with an abundant infiltration of eosinophils, mast cells, CD3+ T lymphocytes, CD79a+ B cells, IgG+ plasma cells and globule leukocytes were recorded in the abomasal mucosa, especially at 7 wpi. Except for the globule leukocytes, this reaction decreased substantially by week 21, suggesting this cell type may have been involved in rejection of adult nematodes. The abomasal lymph nodes showed marked hyperplasia, particularly of CD79a+ B cells and IgG+ plasma cells in all infected goats. These reactions may have been responsible for the reduction in the number of worms found in the abomasum between 3 and 7 wpi.
- Published
- 2001
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205. Immunohistopathologic characterization of a dermal melanocytoma-acanthoma in a German Shepherd Dog.
- Author
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Espinosa de los Monteros A, Martín de las Mulas J, Fernández A, Orós J, and Rodríguez F
- Subjects
- Animals, Carcinoma, Squamous Cell pathology, Carcinoma, Squamous Cell veterinary, Dogs, Female, Keratins analysis, Neoplasms, Glandular and Epithelial pathology, Nevus, Blue pathology, Skin Neoplasms pathology, Dog Diseases pathology, Neoplasms, Glandular and Epithelial veterinary, Nevus, Blue veterinary, Skin Neoplasms veterinary
- Abstract
A cutaneous melanocytoma-acanthoma in a 2-year-old female German Shepherd Dog was characterized by the presence of two populations of neoplastic cells: epithelial and melanocytic. The epithelial component consisted of nests of well-differentiated stratified squamous epithelium closely associated with neoplastic melanocytes. The epithelial cells immunoreacted with both monoclonal and polyclonal anti-cytokeratin antibodies, and immunoreaction to S-100 protein and vimentin was observed in the melanocytic cells. This rare pigmented skin neoplasm of the dog apparently has a benign behavior.
- Published
- 2000
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206. Brain and ocular metastases from a transmissible venereal tumour in a dog.
- Author
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Ferreira AJ, Jaggy A, Varejão AP, Ferreira ML, Correia JM, Mulas JM, Almeida O, Oliveira P, and Prada J
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- Animals, Antineoplastic Combined Chemotherapy Protocols therapeutic use, Brain Neoplasms drug therapy, Brain Neoplasms pathology, Brain Neoplasms secondary, Dog Diseases drug therapy, Dogs, Eye Neoplasms drug therapy, Eye Neoplasms pathology, Eye Neoplasms secondary, Male, Seizures veterinary, Venereal Tumors, Veterinary drug therapy, Brain Neoplasms veterinary, Dog Diseases pathology, Eye Neoplasms veterinary, Venereal Tumors, Veterinary pathology
- Abstract
A five-year-old crossbred dog was referred with rapidly growing masses over its penis and right popliteal lymph node. The dog had severe blepharospasm, congestion of episcleral vessels and rubeosis iridis of the left eye. A presumptive diagnosis of transmissible venereal tumour (TVT) and iridocyclitis was made based on the results of fine needle aspiration. Chemotherapy with vincristine and prednisolone was initiated and after four months the dog made a complete recovery. However, the dog subsequently relapsed, showing miosis, blepharospasm and a well defined mass within the anterior chamber of the left eye. In addition, the dog exhibited generalised 'grand mal' type seizures. Computed tomographic (CT) examination of the brain revealed two distinct masses in the left frontal lobe. Because of the poor prognosis, the owners elected to have the dog euthanased. On histopathology, metastases of TVT in the left eye and left cerebral hemisphere were found, showing no specific staining for CD3, immunoglobulin (Ig) G, IgM and lambda light chains. It was therefore concluded that the tumour growth was progressive, and that there was an absence of local humoral immune response against TVT in this case.
- Published
- 2000
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207. Immunohistochemical analysis of estrogen receptors in feline mammary gland benign and malignant lesions: comparison with biochemical assay.
- Author
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de las Mulas JM, van Niel M, Millán Y, Blankenstein MA, van Mil F, and Misdorp W
- Subjects
- Adenocarcinoma diagnosis, Adenocarcinoma secondary, Adenocarcinoma veterinary, Animals, Antibodies, Monoclonal, Carcinoma, Papillary diagnosis, Carcinoma, Papillary secondary, Carcinoma, Papillary veterinary, Cats, Dilatation, Pathologic diagnosis, Dilatation, Pathologic veterinary, Female, Hyperplasia diagnosis, Hyperplasia veterinary, Immunohistochemistry, Lung Neoplasms secondary, Lymphatic Metastasis, Mammary Glands, Animal chemistry, Mammary Neoplasms, Animal chemistry, Mammary Neoplasms, Animal pathology, Radioligand Assay veterinary, Receptors, Estrogen chemistry, Sensitivity and Specificity, Cat Diseases diagnosis, Mammary Neoplasms, Animal diagnosis, Receptors, Estrogen analysis
- Abstract
Estrogen receptors (ER) were determined by both the biochemical dextran-coated charcoal (DCC-ER) and the immunohistochemical Avidin biotin-peroxidase complex (IHC-ER) methods in proliferative mammary lesions collected from 37 cats: 20 malignant tumors without metastasis at first presentation, seven malignant tumors with lung and/or lymph node metastases and 10 benign tumors and dysplasias. Total number of samples analyzed by both methods was 44. The DCC-ER method was applied to frozen tissue samples and the IHC-ER method was applied to neutral buffered formalin-fixed, paraffin wax-embedded tissue samples by using the NCL-6F11 monoclonal antibody. Biochemically, 21 (47.7%) cases had equal or more than 5 fmol/mg of protein (standard positivity threshold). Immunohistochemically, 11 (25%) cases were scored positive, the percentage of positive nuclei being statistically linked to the intensity of immunostaining. Normal mammary gland tissue (13 cases) and/or dysplastic areas (5 cases) found in the surroundings of the main lesion were IHC-ER positive in 76.9% and 40% of the cases, respectively. Concordance between DCC-ER and IHC-DCC was 72.7% and the results of the DCC and the IHC-ER methods were significantly correlated (P < 0.05) by the chi2 test. Specificity (true negatives) and sensitivity (true positives) of the ICH-ER method were 95.6% and 47.6%, respectively. One out of eleven DCC-ER positive and IHC-ER negative discordant cases (9.09%) was a DCC-ER false positive, because the surrounding normal mammary gland tissue was IHC-ER positive. The remaining 10 cases had ER content values equal or lower than 23 fmol/mg of protein, a figure that could represent the sensitivity threshold of the immunohistochemical method employed.
- Published
- 2000
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208. A new epitope on sheep CD45R molecule detected by a monoclonal antibody.
- Author
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Mateo A, Garrido JJ, Pérez de la Lastra J, Martín de las Mulas J, Moreno A, Pintado CO, and Llanes D
- Subjects
- Animals, Blotting, Western veterinary, Electrophoresis, Polyacrylamide Gel veterinary, Enzyme-Linked Immunosorbent Assay veterinary, Female, Flow Cytometry veterinary, Immunoenzyme Techniques veterinary, Immunohistochemistry, Leukocyte Common Antigens analysis, Leukocyte Common Antigens chemistry, Lymph Nodes cytology, Lymph Nodes immunology, Mice, Mice, Inbred BALB C, Molecular Weight, Spleen cytology, Spleen immunology, Thymus Gland cytology, Thymus Gland immunology, Antibodies, Monoclonal immunology, Epitopes analysis, Leukocyte Common Antigens immunology, Leukocytes, Mononuclear immunology, Sheep immunology
- Abstract
This paper describes the production and characterization of a monoclonal antibody (mAb), Co-46D5, which recognizes a new epitope on the isoform of the homologous sheep leukocyte common antigen (LCA) or CD45. This nmAb was submitted to the 3rd workshop on ruminant leukocyte antigens and was assigned to a cluster reactive with B- and T-cells subsets. Co-46D recognizes a 220 kDa molecule on peripheral blood mononuclear cells (PBMC) and spleen cells but not on thymocytes. Flow cytometry (FCM) analysis shows that Co-46D5 reacted with 30% of PBMC and 50% of spleen cells and more than 95% of cells freshly isolated from lymphoid follicles of the ileal Peyer's patches (IPP) of young lambs. By immunohistochemistry, the antigen was detected mainly on B-cell areas of lymph nodes and spleen. It was also found on a subpopulation of medullar thymocytes. Based on these results, we assume that Co-46D5 recognizes a new epitope on the largest isoform of the sheep CD45 receptor, probably on the homologous to the human CD45RA isoform.
- Published
- 1999
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209. Pathological and immunohistochemical study of the liver and hepatic lymph nodes in goats infected with one or more doses of Fasciola hepatica.
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Pérez J, Martín de las Mulas J, Carrasco L, Gutierrez PN, Martínez-Cruz MS, and Martínez-Moreno A
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- Animals, Fasciola hepatica ultrastructure, Fascioliasis pathology, Gallbladder parasitology, Gallbladder pathology, Goat Diseases pathology, Goats, Liver parasitology, Liver pathology, Lymph Nodes parasitology, Lymph Nodes pathology, T-Lymphocytes cytology, Time Factors, Fascioliasis veterinary, Goat Diseases parasitology
- Abstract
Lesions produced by Fasciola hepatica in the liver, gall-bladder and hepatic lymph nodes (HLNs) of four groups of five goats are described; in addition, the distribution of CD3+ T lymphocytes and IgG-lambda light chain-bearing cells was analysed in the hepatic lesions and HLNs. One group of goats received a single oral dose of metacercariae, but the other four groups received four or five doses at different intervals over a period of 11 weeks. Animals that survived were killed 53-55 weeks after the first infective dose. Goats were more susceptible to multiple doses than to a single dose, even when the total number of metacercariae was the same. This greater susceptibility was manifested by the occurrence of deaths and the severity of hepatic lesions. CD3+ lymphocytes were sparse in the infiltrate surrounding the acute migratory tracts, suggesting inhibition of the local cell-mediated immune response. Goats with numerous hepatic calcareous granulomas showed the most severe hepatic damage, including marked cirrhosis, with a striking infiltrate of CD3+ T lymphocytes and lambda IgG- plasma cells, replacing extensive areas of hepatic parenchyma, in which hypertrophy of the smooth endoplasmic reticulum of hepatocytes was evident. These findings were observed mainly in the goats given more than one infective dose.
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- 1999
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210. Immunohistochemical detection of Brucella abortus antigens in tissues from aborted bovine fetuses using a commercially available polyclonal antibody.
- Author
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Pérez J, Quezada M, López J, Casquet O, Sierra MA, and Martín de las Mulas J
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- Animals, Antibodies, Brucellosis, Bovine embryology, Cattle, Female, Fetus microbiology, Immunohistochemistry, Lung microbiology, Lung pathology, Macrophages pathology, Pregnancy, Retrospective Studies, Abortion, Veterinary microbiology, Antigens, Bacterial analysis, Brucella abortus isolation & purification, Brucellosis, Bovine diagnosis
- Abstract
A commercially available polyclonal antibody and an avidin-biotin-peroxidase immunohistochemical technique were used to detect Brucella abortus antigens in formalin-fixed, paraffin-embedded tissues of lung and liver from 20 aborted bovine fetuses. Thirteen fetuses were obtained from farms with a previous history of brucellosis, and 7 were collected from farms without a history of brucellosis. Among the 13 aborted bovine fetuses obtained from farms with a history of brucellosis, immunoreactivity to B. abortus was detected in lung (9 fetuses) and in liver (1 fetus), whereas Brucella was cultured from abomasal contents in 9 fetuses (8 were immunohistochemically positive). In addition, 11 dams of these 13 aborted bovine fetuses had antibodies to Brucella. Brucella abortus was not detected by immunohistochemistry in the 7 aborted bovine fetuses collected from farms without a history of brucellosis. Bacteriologic culture and serologic tests were also negative for Brucella. The results of this study revealed that the immunohistochemical technique was sufficiently sensitive for detecting B. abortus antigens in formalin-fixed lung tissues from naturally aborted bovine fetuses. Although additional studies are necessary to rule out cross-reaction of the polyclonal antibody with other microorganisms that cause bovine abortion, this immunohistochemical technique could be a complementary tool to serology and bacteriology for the diagnosis of brucellosis.
- Published
- 1998
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211. Development of microscopic lesions in splenic cords of pigs infected with African swine fever virus.
- Author
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Carrasco L, Bautista MJ, Gómez-Villamandos JC, Martin de las Mulas J, Chacón-M de Lara F, Wilkinson PJ, and Sierra MA
- Subjects
- African Swine Fever Virus isolation & purification, African Swine Fever Virus physiology, Animals, Cytopathogenic Effect, Viral, Erythrocytes pathology, Erythrocytes virology, Female, Fibrin metabolism, Macrophages pathology, Macrophages virology, Male, Muscle, Smooth pathology, Muscle, Smooth virology, Spleen virology, Splenomegaly veterinary, Swine, Virion isolation & purification, Virion physiology, Virus Replication, African Swine Fever pathology, Spleen pathology
- Abstract
Acute forms of African swine fever are characterized by hemorrhagic lesions in the lymphoid organs. This paper reports the evolution of lesions in the splenic cords of pigs inoculated with African swine fever (ASF) virus (strain Malawi'83). Ultrastructural examination of the splenic cords of the infected pigs revealed numerous macrophages attached to the muscle cells harboring virus replication center and cytopathic effects at 3 dpi (days post-infection). From 5 dpi, the splenic cords contained a large number of erythrocytes associated with abundant fibrin deposits, mainly arranged around the muscle cells, from which macrophages had disappeared. It is likely that the ASF virus replication, and consequent cytopathic effects, observed in the fixed macrophages of splenic cords, may be responsible for the fibrin deposition.
- Published
- 1997
212. Immunohistochemical detection of hog cholera viral glycoprotein 55 in paraffin-embedded tissues.
- Author
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de las Mulas JM, Ruiz-Villamor E, Donoso S, Quezada M, Lecocq C, and Sierra MA
- Subjects
- Animals, Brain pathology, Brain virology, Cattle, Diagnosis, Differential, Digestive System pathology, Digestive System virology, Immunohistochemistry methods, Lymphoid Tissue pathology, Lymphoid Tissue virology, Reference Values, Respiratory System pathology, Respiratory System virology, Skin pathology, Skin virology, Swine, Urinary Tract pathology, Urinary Tract virology, Classical Swine Fever pathology, Classical Swine Fever Virus isolation & purification, Viral Envelope Proteins analysis
- Abstract
Formalin-fixed, paraffin embedded tissues obtained from 40 pigs inoculated with a field isolate of hog cholera virus were examined for the presence of Gp55, a major structural protein of the virus envelope, using a monoclonal antibody-based immunohistochemical test with the avidin-biotin-peroxidase complex method. Immunoreactivity was detected in hog cholera virus-infected tissues but not in control pigs tissues, African swine fever virus-infected tissues, or bovine viral diarrhea virus-infected porcine or bovine tissues. The first positive reactions were seen in lymphatic tissues, digestive tract and skin on postinoculation day (pid) 4, respiratory and urinary tissues on pid 5, nervous tissues on pid 6, and endocrine tissues on pid 7. These staining reactions persisted until the last observation on pid 18. Hog cholera virus antigen was not detected in heart tissue at any time. The highest levels of antigen detection were found in tonsils, spleen, and pancreas, although the esophageal mucosa and skin epithelial cells were also intensely and widely stained. The cellular staining pattern of Gp55 had a ubiquitous distribution. It was found in epithelial cells, macrophages and circulating monocytes, endothelial cells, lymphoid cells, and glial cells. The results showed a high specificity and high sensitivity for detecting hog cholera Gp55 in formalin-fixed, paraffin embedded tissue samples. This method allows precise association of Gp55 with specific cells, tissues, and histologic lesions, making the technique suitable for use in routine diagnosis of hog cholera.
- Published
- 1997
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213. Fibrillary astrocytoma in a goat: pathologic, immunohistochemical, and ultrastructural study.
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Martín de las Mulas J, Bautista MJ, de Lara FC, and Carrasco L
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- Animals, Astrocytoma pathology, Astrocytoma ultrastructure, Blindness, Brain pathology, Brain Neoplasms pathology, Brain Neoplasms ultrastructure, Glial Fibrillary Acidic Protein analysis, Goats, Immunohistochemistry, Keratins analysis, Motor Activity, Nerve Tissue Proteins analysis, Neurofilament Proteins analysis, Vimentin analysis, Astrocytoma veterinary, Brain Neoplasms veterinary, Goat Diseases
- Published
- 1996
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214. Intestinal smooth muscle hyperplasia in a goat.
- Author
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de Lara FC, Hervás J, Bautista MJ, Pérez J, Gómez-Villamandos JC, Martín de las Mulas J, and Carrasco L
- Subjects
- Animals, Female, Goats, Hyperplasia, Inflammation pathology, Inflammation veterinary, Goat Diseases, Intestine, Small pathology, Muscle, Smooth pathology
- Published
- 1996
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215. A monoclonal antibody to ruminant fibrinogen produced through immunization with ovine peripheral blood leukocytes.
- Author
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Pintado CO, Moreno A, Perez de la Lastra J, Martin de las Mulas J, and Llanes D
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- Animals, Antibodies, Monoclonal chemistry, Cattle, Female, Flow Cytometry, Goats, Horses, Immunization, Passive, Immunohistochemistry, Mice, Mice, Inbred BALB C, Species Specificity, Swine, Antibodies, Monoclonal biosynthesis, Fibrinogen immunology, Granulocytes immunology, Monocytes immunology, Sheep immunology
- Abstract
By immunizing BALB/c mice with peripheral blood leukocytes (PBL) we have obtained a monoclonal antibody (MAb) named Co.1C9D10, specific for the gamma chain of ruminant fibrinogen. This MAb immunoprecipitates a molecule of apparent molecular weight of 55/65 kDa under reducing conditions and of around 300 kDa under nonreducing conditions. Co.1C9D10 recognized the gamma chain of fibrinogen by immunoblotting techniques and blood vessel plasma from paraffin embedded tissues by immunocytological techniques. Flow cytometry analysis showed that fibrinogen appears adhered to monocytes and granulocytes being eliminated through EDTA treatment of the cells. This phenomenon explains why PBL used as immunogen was able to induce an MAb against fibrinogen.
- Published
- 1996
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216. In vivo replication of African swine fever virus (Malawi '83) in neutrophils.
- Author
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Carrasco L, Gómez-Villamandos JC, Bautista MJ, Martín de las Mulas J, Villeda CJ, Wilkinson PJ, and Sierra MA
- Subjects
- African Swine Fever Virus isolation & purification, African Swine Fever Virus ultrastructure, Animals, Antibodies, Viral biosynthesis, Bone Marrow pathology, Bone Marrow ultrastructure, Bone Marrow virology, Endoplasmic Reticulum pathology, Endoplasmic Reticulum ultrastructure, Endoplasmic Reticulum virology, Immunohistochemistry, Liver pathology, Liver ultrastructure, Malawi, Microscopy, Electron, Neutrophils physiology, Neutrophils ultrastructure, Swine, African Swine Fever pathology, African Swine Fever Virus physiology, Neutrophils virology, Virus Replication
- Abstract
The presence of virus replication centers in neutrophils from pigs inoculated with a highly virulent strain of African swine fever virus is described for the first time in vivo. Virus antigens were observed from 3 days post-inoculation (dpi) onwards by means of an immunohistochemical technique. At this time (3 dpi), transmission electron microscopy studies revealed the presence of large amounts of neutrophils in the vascular lumens. At 5 and 7 dpi, neutrophils with phagosomes frequently contained virus particles. In addition, within the cytoplasm of some mature and immature neutrophils, both viral particles and virus replication centers were observed at 5 and 7 dpi.
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- 1996
217. Virus association with lymphocytes in acute African swine fever.
- Author
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Carrasco L, de Lara FC, Martín de las Mulas J, Gómez-Villamandos JC, Hervás J, Wilkinson PJ, and Sierra MA
- Subjects
- African Swine Fever pathology, African Swine Fever virology, Animals, Female, Lymph Nodes pathology, Lymph Nodes ultrastructure, Lymph Nodes virology, Macrophages virology, Malawi, Male, Swine, Time Factors, African Swine Fever immunology, African Swine Fever Virus isolation & purification, African Swine Fever Virus physiology, Lymphocytes virology, Macrophages immunology, Virus Replication
- Abstract
This paper reports the presence of mature viral particles within the lymphocytes of samples taken from pigs inoculated with a highly virulent African swine fever (ASF) virus isolate (Malawi 83), and the adhesion of the lymphocytes to macrophages containing the virus replication sites. Virus replication in lymph-node medullar tissue macrophages was observed from 3 days post inoculation (pi). At 3 days pi, transmission electron microscopy revealed hemadsorption in some infected macrophages. At 5 and 7 days pi, a number of macrophages with virus replication were surrounded by a ring of lymphocytes. In such cases, mature viral particles were observed in membrane evaginations of the infected cell that corresponded to invaginations of the lymphocyte membrane. Also at 5 and 7 days pi, mature virions were observed within the cytoplasm of some lymphocytes. However, incomplete virions and African swine fever virus replication sites were not observed within the lymphocytes.
- Published
- 1996
218. Immunohistochemical distribution pattern of intermediate filament proteins in 50 feline neoplasms.
- Author
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Martín de las Mulas J, Espinosa de los Monteros A, Carrasco L, van Niel M, and Fernández A
- Subjects
- Animals, Cat Diseases pathology, Cats, Desmin analysis, Desmin metabolism, Glial Fibrillary Acidic Protein analysis, Glial Fibrillary Acidic Protein metabolism, Immunohistochemistry, Intermediate Filament Proteins metabolism, Keratins analysis, Keratins metabolism, Kidney chemistry, Kidney pathology, Kidney Neoplasms chemistry, Kidney Neoplasms pathology, Kidney Neoplasms veterinary, Lung Neoplasms chemistry, Lung Neoplasms pathology, Lung Neoplasms veterinary, Neoplasms chemistry, Neoplasms, Glandular and Epithelial chemistry, Neoplasms, Glandular and Epithelial pathology, Neurofilament Proteins analysis, Neurofilament Proteins metabolism, Skin Neoplasms chemistry, Skin Neoplasms pathology, Skin Neoplasms veterinary, Thyroid Gland chemistry, Thyroid Gland pathology, Thyroid Neoplasms chemistry, Thyroid Neoplasms pathology, Thyroid Neoplasms veterinary, Vimentin analysis, Vimentin metabolism, Cat Diseases metabolism, Intermediate Filament Proteins analysis, Neoplasms veterinary, Neoplasms, Glandular and Epithelial veterinary
- Abstract
Twenty-eight epithelial and 22 nonepithelial feline tumors were studied immunohistochemically. Epithelial tumors were 10 squamous cell carcinomas, two basal cell tumors, two sebaceous gland carcinomas, three apocrine gland carcinomas, three thyroid papillary carcinomas, one thyroid solid carcinoma, one renal clear cell carcinoma, one renal papillary carcinoma, one endometrial carcinoma, and four lung bronchioloalveolar carcinomas. Nonepithelial tumors were 10 fibrosarcomas, one liposarcoma, one leiomyosarcoma, one rhabdomyosarcoma, one hemangiosarcoma, two mast cell tumors, one osteosarcoma, three melanomas, and two lymphomas. Commercially available antibodies directed against high- and low-molecular-weight keratins (keratin, RCK-102, NCL-5D3), vimentin, desmin, glial fibrillary acidic protein (GFAP), and neurofilament intermediate filament (IF) proteins were used in the avidin-biotin-peroxidase complex technique on formalin-fixed, paraffin-embedded tumor tissue samples. All epithelial tumors except the endometrial carcinoma expressed some type of keratin protein. Squamous cell carcinomas expressed high-molecular-weight keratins exclusively. Coexpression of high- and low-molecular-weight keratins was observed in one basal cell tumor, sebaceous and apocrine adenocarcinomas, and thyroid, renal, and lung carcinomas. In addition to keratins, vimentin immunoreactivity was found in all basal cell tumors, all sebaceous gland, thyroid papillary, renal, and lung adenocarcinomas, and one of the apocrine gland adenocarcinomas. Immunoreactivity with GFAP antibody was found in one basal cell tumor and one sebaceous gland adenocarcinoma. The endometrial carcinoma did not react with any of the antibodies applied. Nonepithelial tumors analyzed expressed either vimentin (fibrosarcomas, liposarcoma, haemangiosarcoma, mast cell tumors, osteosarcomas, melanomas) or vimentin and desmin (leiomyosarcoma, rhabdomyosarcoma, one fibrosarcoma) IF proteins exclusively. Lymphomas did not react with any of the antibodies employed. These findings indicate that IF proteins antibodies can be included in diagnostic panels of antibodies for immunocharacterization of feline tumors. In addition, they can be used as a basis for the diagnoses of poorly differentiated or undifferentiated feline neoplasms.
- Published
- 1995
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219. Description of a new population of fixed macrophages in the splenic cords of pigs.
- Author
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Carrasco L, Bautista MJ, Martin de las Mulas J, Gómez-Villamandos JC, Espinosa de los Monteros A, and Sierra MA
- Subjects
- Actins analysis, Animals, Cell Adhesion, Cell Membrane ultrastructure, Cytoplasm ultrastructure, Immunohistochemistry, Intercellular Junctions ultrastructure, Macrophages physiology, Microscopy, Electron, Microscopy, Electron, Scanning, Muscle, Smooth chemistry, Muscle, Smooth ultrastructure, Perfusion, Spleen chemistry, Macrophages ultrastructure, Spleen ultrastructure, Swine immunology
- Abstract
The ultrastructure of porcine splenic cords was analysed by light and electron microscopy after both perfusion and immersion fixation. The external aspect of the splenic cords was found to be composed of a network of smooth muscle cells arranged between the trabeculae and the venous sinus walls. Macrophages frequently attached to smooth muscle cells surrounded the whole of their surfaces and were attached by slender cytoplasmic projections. Intercellular junctions in the form of electron-dense areas 200-350 nm in length and 45-55 nm in thickness were observed in the macrophage membrane close to the muscle cells. In the splenic cords of nonperfused animals, in addition to the macrophage population adhering to smooth muscle cells, numerous macrophages without cell junctions were also seen and identified as free macrophages.
- Published
- 1995
220. Detection of African swine fever virus protein VP73 in tissues of experimentally and naturally infected pigs.
- Author
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Pérez J, Rodríguez F, Fernández A, Martín de las Mulas J, Gómez-Villamandos JC, and Sierra MA
- Subjects
- African Swine Fever immunology, African Swine Fever pathology, Animals, Antigens, Viral isolation & purification, Swine, African Swine Fever virology, African Swine Fever Virus immunology, Swine, Miniature, Viral Proteins isolation & purification
- Published
- 1994
- Full Text
- View/download PDF
221. Immunohistochemical distribution of keratin proteins in feline tissues.
- Author
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de las Mulas J, Espinosa de los Monteros A, Gomez-Villamandos JC, Fernandez A, and Vos JH
- Subjects
- Animals, Digestive System metabolism, Female, Genitalia metabolism, Immunohistochemistry, Male, Mammary Glands, Animal metabolism, Respiratory System metabolism, Skin metabolism, Tissue Distribution, Urinary Tract metabolism, Cats metabolism, Keratins metabolism
- Abstract
The immunohistochemical distribution pattern of some keratin intermediate filament proteins has been analysed in a wide range of formalin fixed, paraffin embedded feline tissues using one polyclonal and two monoclonal antibodies raised against human keratins by means of the avidin-biotin-peroxidase complex technique. Only the epithelial and mesothelial cells were stained by the three antibodies, but differences in their corresponding staining pattern were noticed. The staining reaction of the polyclonal antibody raised against human skin keratin was found in both stratified and complex epithelia, while that of the monoclonal antibody which recognizes human keratins 8 + 18 + 19 of the Moll catalogue (NCL-5D3) was restricted to some simple epithelia. The staining reaction of the monoclonal antibody which reacts with human keratins 5 + 8 of the Moll catalogue (RCK-102) covered the widest spectrum of feline epithelial tissues analysed, including stratified, complex and simple epithelia. These staining patterns of feline tissues are basically similar with respect to those of corresponding tissues in other mammalian species, although some differences were also noticed and some obvious epithelial tissues were not stained. This study confirms the broad interspecies cross-reactivity of keratin proteins antibodies and demonstrates their capability to differentiate between various types of feline epithelia and some epithelial compartments.
- Published
- 1994
- Full Text
- View/download PDF
222. Immunohistochemical distribution of vimentin, desmin, glial fibrillary acidic protein and neurofilament proteins in feline tissues.
- Author
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Martín de las Mulas J, Espinosa de los Monteros A, Carrasco L, Sierra MA, and Vos JH
- Subjects
- Animals, Female, Immunohistochemistry, Male, Cats metabolism, Desmin analysis, Glial Fibrillary Acidic Protein analysis, Neurofilament Proteins analysis, Vimentin analysis
- Abstract
The immunohistochemical distribution pattern of vimentin, desmin, glial fibrillary acidic protein and neurofilaments intermediate filament proteins has been analyzed in a wide range of formalin fixed, paraffin embedded tissues using polyclonal and monoclonal antibodies raised against non-feline antigens. The vimentin antibody reacted with mesenchymal cells, the desmin antibody with striated and smooth muscle fibres, the glial fibrillary acidic protein antibody with glial cells in the central and peripheral nervous system, and the neurofilament proteins antibody with neuronal cell bodies and processes. In addition, some epithelial cells were vimentin positive, perisinusoidal liver cells were desmin positive, and basal/myoepithelial cells of the mammary gland, and luteinic cells were glial fibrillary acidic protein positive. These staining patterns of feline tissues are basically similar with respect to that of corresponding tissues in other mammalian species for each of the four intermediate filament proteins studied, but some differences have been also noticed. This study confirms the broad interspecies cross-reactivity of intermediate filament proteins antisera and demonstrates their capability to differentiate particular types of feline cells and tissues.
- Published
- 1994
- Full Text
- View/download PDF
223. Multiple cutaneous capillary haemangioma in a red partridge (Alectoris rufa).
- Author
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Mulas JM, Gomez-Villamandos JC, Perez J, Carrasco L, and Mendez A
- Abstract
A 6-year-old healthy decoy red partridge (Alectoris rufa) developed rapidly growing multiple cutaneous nodules in the dorsal aspect of the neck. Histologically, they were distinctly exophytic growths composed of variably-sized blood vessels often surrounded by solid masses of endothelial-like cells set in an oedematous stroma with abundant inflammatory cells. They were diagnosed as multiple cutaneous capillary haemangiomas. These tumours have been described in chickens in association with viral infections and in old budgerigars, but not in partridges.
- Published
- 1993
- Full Text
- View/download PDF
224. Desmin and vimentin immunocharacterization of feline muscle tumors.
- Author
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Martín de las Mulas J, Vos JH, and Van Mil FN
- Subjects
- Animals, Cats, Glial Fibrillary Acidic Protein analysis, Immunohistochemistry, Neurofilament Proteins analysis, Rhabdomyosarcoma chemistry, Soft Tissue Neoplasms chemistry, Cat Diseases pathology, Desmin analysis, Muscles chemistry, Rhabdomyosarcoma veterinary, Soft Tissue Neoplasms veterinary, Vimentin analysis
- Published
- 1992
- Full Text
- View/download PDF
225. Immunohistological diagnosis of rabbit haemorrhagic disease in experimentally infected rabbits in Spain.
- Author
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Carrasco L, Rodriguez F, Martin de las Mulas J, Wohlsein P, and Fernandez A
- Subjects
- Animals, Cross Reactions, Hemorrhage diagnosis, Immunohistochemistry, Parvoviridae Infections diagnosis, Hemorrhage veterinary, Parvoviridae immunology, Parvoviridae Infections veterinary, Rabbits
- Abstract
The immunoreactivity of routinely processed liver and lung tissue samples obtained from rabbits inoculated with tissue explants from naturally infected animals when antisera directed against parvovirus from different species (canine, feline and porcine) as well as a RHD virus antiserum were employed has been tested by different immunoperoxidase methods. Cross-reactivity between RHD-virus antigens and parvovirus antigens was present. Best results were obtained with RHD and canine parvovirus antisera with the ABC method. The immunoreactivity in the liver was found in hepatocytes, Kupffer and bile duct cells. In the lung, it was exclusively observed in intravascular macrophages.
- Published
- 1991
- Full Text
- View/download PDF
226. Caprine peripheral neuroblastomas: structural and ultrastructural features, intermediate filaments profile and neuro-endocrine characterization.
- Author
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Martin de las Mulas J, Vos JH, Fernández A, Carrasco L, Mozos E, and Sierra MA
- Subjects
- Animals, Cytoplasmic Granules chemistry, Cytoplasmic Granules ultrastructure, Goat Diseases metabolism, Goats, Neoplasms, Multiple Primary chemistry, Neoplasms, Multiple Primary pathology, Neuroblastoma chemistry, Neuroblastoma pathology, Phosphopyruvate Hydratase analysis, Retroperitoneal Neoplasms chemistry, Retroperitoneal Neoplasms pathology, Synaptophysin analysis, Vimentin analysis, Biomarkers, Tumor analysis, Goat Diseases pathology, Intermediate Filament Proteins analysis, Neoplasm Proteins analysis, Neoplasms, Multiple Primary veterinary, Neuroblastoma veterinary, Neurofilament Proteins analysis, Retroperitoneal Neoplasms veterinary
- Abstract
In this report the morphological and immunohistochemical features with respect to intermediate filament proteins and the neuroendocrine nature of bilateral neuroblastomas possibly originating from the adrenal medulla in a goat kid are presented. Histologically, the tumours were composed of small, round, blue cells organized in highly cellular nests and sheets separated by fibrovascular septa. Isolated cells mimicking the morphological features of neurones were observed in both tumours. Ultrastructurally, dense-core neurosecretory granules, about 100 nm in diameter, and irregularly organized neurotubular networks were seen. The small tumour cells were only labelled by vimentin, while the neurone-like cells were labelled by both neurone-specific enolase and synaptophysin. The lack of staining of the tumour cells by the neurofilament proteins antiserum, which was also observed in a human neuroblastoma (used as positive control) has been previously reported and is probably the result of the tissue processing and/or the poor differentiation stage of the tumours. The vimentin labelling of tumour cells could be explained by the poor differentiation stage of the tumours, since vimentin is the only intermediate filament protein in presumptive neuroblasts, being replaced by neurofilament proteins in later stages of neurogenesis.
- Published
- 1991
- Full Text
- View/download PDF
227. Intracystic carcinoma of the breast. A clinicopathologic entity.
- Author
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Morales-Jimenez C, Reymundo C, Lopez-Beltran A, Martin de las Mulas J, and Toro-Rojas M
- Subjects
- Aged, Aged, 80 and over, Breast Neoplasms surgery, Carcinoma, Papillary surgery, Female, Humans, Immunohistochemistry, Lymph Node Excision, Mastectomy, Breast Neoplasms pathology, Carcinoma, Papillary pathology
- Abstract
Two intracystic breast carcinomas with papillary pattern, one of which was argyrophilic, are reported, and their peculiar clinical features emphasized. The confusing terminology used in literature is also discussed. The fainly well-defined clinico-pathological features of these tumors allow them to be classed as a specific clinico-pathological variety of ductal breast carcinoma. The immunohistochemical study, which was negative to ALA, ACTH, GH, PRL and NSE, led us to consider the potential mucoid nature of the argyrophilic granules.
- Published
- 1991
228. [A retrospective study of infection in patients with malignant haemopathies and neutropenia (author's transl)].
- Author
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Díaz Mediavilla J, Espinos Pérez D, Rodríguez Vázquez M, Martín de las Mulas J, Peñalver Díaz M, Marin Ferrer D, and De la Torre F
- Subjects
- Adolescent, Adult, Age Factors, Bacterial Infections epidemiology, Child, Child, Preschool, Female, Hospitalization, Humans, Infant, Male, Middle Aged, Time Factors, Agranulocytosis complications, Bacterial Infections etiology, Leukemia complications, Neutropenia complications
- Published
- 1979
229. Galactorrhea associated with lymphocytic adenohypophysitis. Case report.
- Author
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Cebelin MS, Velasco ME, de las Mulas JM, and Druet RL
- Subjects
- Adult, Female, Humans, Inflammation complications, Inflammation pathology, Lymphocytes pathology, Pituitary Diseases pathology, Pituitary Gland, Anterior pathology, Pregnancy, Prolactin, Galactorrhea etiology, Lactation Disorders etiology, Pituitary Diseases complications
- Abstract
A young woman, 14 months post partum, committed suicide when she noticed milk discharging from her nipples and thought she was pregnant. At autopsy the uterus was found to be non-gravid and the pituitary gland enlarged. Histological examination revealed lymphocytic adenohypophysitis, a rarely identified condition usually associated with hypopituitarism. Immunoperoxidase studies of the pituitary demonstrated prolactin cell hyperplasia. None of the other known causes of galactorrhea was present suggesting that this may be a new addition to the differential diagnosis of galactorrhea. To this date lymphocytic adenohypophysitis has been reported only in women, the majority within 14 months postpartum, and is usually identified only at autopsy.
- Published
- 1981
- Full Text
- View/download PDF
230. Immunohistological identification of both infectious bursal and Marek virus antigens in the bursa of Fabricius.
- Author
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Fernández A, Martín de las Mulas J, Sierra MA, Carranza J, and Jover A
- Subjects
- Animals, Marek Disease complications, Poultry Diseases microbiology, Reoviridae Infections complications, Reoviridae Infections veterinary, Antigens, Viral analysis, Bursa of Fabricius microbiology, Chickens, Herpesvirus 2, Gallid immunology, Infectious bursal disease virus immunology, Reoviridae immunology
- Abstract
Indirect Immunoperoxidase (IIP) and Avidin Biotin-Peroxidase Complex (ABC) techniques were used for the detection of Infectious Bursal Virus (IBV) and Marek Disease Virus (MDV) antigens in alcohol and formalin-fixed, paraffin-embedded lymphoid tissues from broilers. Both techniques appeared potentially useful for the diagnosis of both viral antigens in alcohol-fixed tissues, and allowed the observation of dual infection in the bursa of Fabricius of the studied animals in a natural infection.
- Published
- 1989
231. Immunohistochemical localization of prolactin in functioning and regressing corpus luteum of pituitary autotransplanted rats.
- Author
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Martín de las Mulas J, Aguilar E, and Sánchez-Criado JE
- Subjects
- Animals, Bromocriptine administration & dosage, Corpus Luteum ultrastructure, Female, Immunohistochemistry, Luteolysis drug effects, Organ Size, Ovary physiology, Pituitary Gland chemistry, Progesterone analysis, Progesterone blood, Prolactin analysis, Prolactin blood, Rats, Rats, Inbred Strains, Corpus Luteum physiology, Luteal Cells chemistry, Luteolysis physiology, Pituitary Gland physiology, Prolactin physiology
- Abstract
In an attempt to shed light on the intimate mechanism by which prolactin (PRL) switches from supporting corpus luteum (CL) progesterone secretion (P) to promote structural regression of the CL, day 2 (metestrous) autopituitary transplanted (APTr) rats were used. In APTr rats the CL is under the only control of PRL since an almost complete absence of LH and FSH exist. The experimental group was given bromocriptine (CB-154: 0.4 mg/day) on days 12, 13 and 14 of the cycle and 0.25 ml of ethanol from day 15 to day 21. The control group was given CB-154 from day 12 to day 21. Rats were hemiovariectomized on day 12 to assess the morphological characteristics of the active CL. PRL and P were determined by RIA on days 12, 15 and 22. On day 12, both PRL and P levels were higher than 80 ng/ml (luteotrophic action of PRL). On day 15, due to treatment with CB-154, the levels of both hormones had fallen below 7 ng/ml (functional luteolysis). On day 22, PRL levels were again high (greater than 50 ng/ml) in the shortly CB-154-treated rats and low (less than 5 ng/ml) in the controls; the P levels were lower than 5 ng/ml in both groups. PRL-induced structural luteolysis in the experimental group (hyperprolactinemic) was assessed by the structural characteristics and by the CL weight loss on day 22 in comparison with that exhibited by control rats. The immunohistochemical staining of both endogenous and total PRL in the lutein cells showed that the internalization of PRL is not modified by the functional state of the CL, nevertheless the intracellular redistribution of the internalized hormone varied in relation with the PRL action on the CL (luteotrophic, day 12 vs luteolytic, day 22). These results seem to indicate that intracellular mechanisms rather than receptor content determine CL response to PRL.
- Published
- 1986
232. [Coexistence of multilocular cyst and adenocarcinoma in the same kidney].
- Author
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Morales Jiménez C, Martín de las Mulas J, García Calvo J, López Beltrán A, and Reymundo García C
- Subjects
- Aged, Humans, Male, Adenocarcinoma pathology, Cysts pathology, Kidney Neoplasms pathology, Neoplasms, Multiple Primary
- Published
- 1987
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