351. Reactive oxygen species-quenching and anti-apoptotic effect of polaprezinc on indomethacin-induced small intestinal epithelial cell injury.
- Author
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Omatsu T, Naito Y, Handa O, Mizushima K, Hayashi N, Qin Y, Harusato A, Hirata I, Kishimoto E, Okada H, Uchiyama K, Ishikawa T, Takagi T, Yagi N, Kokura S, Ichikawa H, and Yoshikawa T
- Subjects
- Animals, Anti-Inflammatory Agents, Non-Steroidal toxicity, Apoptosis drug effects, Carnosine pharmacology, Cell Line, Epithelial Cells drug effects, Epithelial Cells metabolism, Intestinal Mucosa drug effects, Intestinal Mucosa pathology, Oxidation-Reduction drug effects, Oxidative Stress drug effects, Protein S drug effects, Protein S metabolism, Rats, Zinc Compounds pharmacology, Anti-Ulcer Agents pharmacology, Carnosine analogs & derivatives, Indomethacin toxicity, Organometallic Compounds pharmacology, Reactive Oxygen Species metabolism
- Abstract
Background: To protect the small intestine from mucosal injury induced by nonsteroidal anti-inflammatory drugs is one of the critical issues in the field of gastroenterology. Polaprezinc (PZ), a gastric muco-protecting agent, has been widely used for the treatment of gastric ulcer and gastritis for its unique effects, such as its strong reactive oxygen species (ROS)-quenching effect. The aim of this study was to clarify the mechanism by which indomethacin-induced small intestinal mucosal injury occurs, by using a rat intestinal epithelial cell line (RIE-1). In addition, the protective role of PZ and the possible mechanism of its effect on indomethacin-induced small intestinal injury were investigated., Methods: Cell death was evaluated by methyl thiazolyl tetrazolium (MTT) assay and a double-staining method with Hoechst33342 dye and propidium iodide. Indomethacin-induced ROS production was evaluated by detecting the oxidation of a redox-sensitive fluorogenic probe, RedoxSensor, and the oxidation of cysteine residues of proteins (protein S oxidation). The activation of cytochrome c, smac/DIABLO, and caspase-3 was assessed by western blotting. In some experiments, PZ or its components, L: -carnosine and zinc, were used., Results: We found that indomethacin caused apoptosis in RIE-1 cells in a dose- and time-dependent manner. Indomethacin also induced ROS production and an increase in the protein S oxidation of RIE-1. Pretreatment of RIE-1 with PZ or zinc sulfate, but not L: -carnosine, significantly reduced the indomethacin-induced apoptosis. PZ prevented ROS production and the increase in protein S-oxidation. PZ inhibited indomethacin-induced cytochrome c and smac/DIABLO release and subsequent caspase-3 activation., Conclusions: The protective effect of PZ on indomethacin-induced small intestinal injury may be dependent on its ROS-quenching effect. more...
- Published
- 2010
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