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Objective: To investigate the effects of Angelica sinensis polysaccharide (AP) and its three fractions (AP-1, AP-2 and AP-3) on lymphocyte proliferation induction of IL-2 and IFN-gamma., Methods: Effects of AP on proliferation of murine splenocyte, macrophage, mixed lymphocyte reaction were tested by MTT assay. The production of IL-2 and IFN-gamma in supernatant were determined by ELISA., Results: At the range of 30 - 300 microg/ml, AP and its three fractions directly stimulated proliferation of murine splenocyte; AP-3 significantly enhanced the proliferation of macrophage and mixed lymphocyte, the treatment of AP-3 augmented the production of IL-2 and IFN-gamma in cell culture supernatant., Conclusion: AP could active macrophage and T lymphocyte, also regulate the immune response through upregulating IL-2, IFN-gamma expression.

Objective: To observe the effects of oral administration of guanxin II decoction (GX II) on cardiovascular function, especially on the dynamics of coronary blood flow in healthy males., Methods: Changes of heart rate, diastolic pressure, systolic pressure, left ventricular ejection fraction (LVEF), E peak, A peak, E/A value of mitral flow, diastolic peak velocity (Vmax) and diastolic flow velocity time integrals (VTI) of left anterior descending coronary artery (LAD) in 11 healthy male subjects were measured before and after oral administration of GX II, using non-invasive echocardiogram., Results: Compared with those before GX II administration, the changes after administration in heart rate, systolic pressure, diastolic pressure, LVEF, E peak, A peak and E/A value, were insignificantly different (P>0.05), but the Vmax and VTI significantly increased at 30 min, 60 min, 90 min and 120 min after GX II administration (P<0.05)., Conclusion: To increase the coronary blood flow is possibly one of the mechanisms of GX II in treating coronary heart disease and angina pectoris.

Objective: To investigate the effects of angelica polysaccharide (AP) on blood coagulation and platelet aggregation., Methods: Infrared turbidimetric method was used to estimate platelet aggregation, active partial thromboplastin time (APTT), prothrombin time (PT) and thrombin time (TT); bleeding time (BT) was measured by cutting the mouse's tail and coagulate time (CT) was measured by dropping the blood on carry sheet glass., Results: AP prominently enhanced the platelet aggregation at 5 min, while showed less effects on the maximum platelet aggregation. It also markedly prolonged CT but shortened BT. AP significantly prolonged TT and APTT in dosage of 3 mg/kg and 10 mg/kg, while showed no obvious effect on PT., Conclusion: These results suggest that AP has potent anticoagulant and haemostasis effects. The haemostasis effect is related to promoting platelet aggregation.