Author: "M Glas" - Searchworks@Jio Institute Digital Library Search Results

Author: Willems H, Thiele D, Glas-Adollah-Baik Kashi M, and Krauss H
Subjects: Animals, Antigens, Bacterial immunology, Blotting, Western, Cattle, Coxiella burnetii immunology, Densitometry, Electrophoresis, Polyacrylamide Gel, Humans, Mice, Q Fever immunology, Antibodies, Bacterial analysis, Immunoblotting methods, Q Fever diagnosis
Abstract: Partially purified but unfixed C. burnetii cells were submitted to sodium dodecylsulfate polyacrylamide gel electrophoresis, Western blotted and used as antigens for evaluation of antibody responses against the agent in mice, cattle and humans. Increased specificity was achieved by using glutaraldehyde as additional blocking agent. Gels as well as membranes were subjected to laser densitometry and data analysed with an IBM personal computer. This technique offers the possibility to objectively quantify and document the results of immunoblotting.
Published: 1992
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Author: Thiele D, Willems H, and Glas M
Subjects: Animals, Antibodies, Bacterial immunology, Densitometry, Enzyme-Linked Immunosorbent Assay, Immunoblotting, Immunoglobulin G immunology, Lasers, Mice, Mice, Inbred BALB C, Q Fever microbiology, Antibodies, Bacterial biosynthesis, Coxiella burnetii immunology, Immunoglobulin G biosynthesis, Q Fever immunology
Abstract: Balb/c mice were inoculated with live or inactivated organisms of Coxiella burnetii, strain Nine Mile, phase I. Sera collected after different time intervals were subjected to immunoblot analysis and results compared with ELISA values. Immunoblots were performed with different horseradish peroxidase labelled conjugates (goat anti-mouse IgG1, IgG2a, IgG2b, IgG3) and results monitored and analysed by laser densitometry. ELISA analysis was performed using the same peroxidase labelled goat anti-mouse subclass antibody conjugates. In addition, goat anti-mouse IgG(H+L), IgG(H), IgM, and IgA conjugates were used for ELISA tests.
Published: 1992

Author: De Marzo C, De Palma M, Favuzzi C, Maggi G, Nappi E, Posa F, Ranieri A, Selvaggi G, Spinelli P, Bamberger A, Fuchs M, Heck W, Loos C, Marx R, Runge K, Skodzek E, Weber C, Wülker M, Zetsche F, Artemiev V V, Galaktionov Y, Gordeev A, Gorodkov Y, Kamyshkov Y, Plyaskin V V, Pojidaev V V, Shevchenko V V, Shumilov E, Tchudakov V V, Bunn J, Fent J, Freund P, Gebauer J, Glas M, Polakos P, Pretzl K, Schouten T, Seyboth P, Seyerlein J, and Vesztergombi G
Published: 1990
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Author: De Marzo C, De Palma M, Favuzzi C, Maggi G, Nappi E, Posa F, Ranieri A, Selvaggi G, Spinelli P, Bamberger A, Fuchs M, Heck W, Loos C, Marx R, Runge K, Skodzek E, Weber C, Wülker M, Zetsche F, Artemiev V V, Galaktionov Y, Gordeev A, Gorodkov Y, Kamyshkov Y, Kossov M, Plyaskin V V, Pojidaev V V, Shevchenko V V, Shumilov E, Tchudakov V V, Bunn J, Fent J, Freund P, Gebauer J, Glas M, Polakos P, Pretzl K, Schouten T, Seyboth P, Seyerlein J, and Vesztergombi G
Published: 1987
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Author: De Marzo C, De Palma M, Favuzzi C, Maggi G, Nappi E, Posa F, Ranieri A, Selvaggi G, Spinelli P, Bamberger A, Fuchs M, Heck W, Loos C, Marx R, Runge K, Skodzek E, Weber C, Wülker M, Zetsche F, Artemiev V V, Galaktionov Y, Gordeev A, Gorodkov Y, Kamyshkov Y, Plyaskin V V, Pojidaev V V, Shevchenko V V, Shumilov E, Tchudakov V V, Bunn J, Fent J, Freund P, Gebauer J, Glas M, Polakos P, Pretzl K, Schouten T, Seyboth P, Seyerlein J, and Vesztergombi G
Published: 1987
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