318 results on '"Le Roy, Pascale"'
Search Results
302. Selection for reduced muscle glycolytic potential in Large White pigs I. Direct responses
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Le Roy, Pascale, Larzul, Catherine, Gogué, Jean, Talmant, André, Monin, Gabriel, and Sellier, Pierre
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- 1998
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303. Nest acceptance, clutch, and oviposition traits are promising selection criteria to improve egg production in cage-free system.
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Becot, Lorry, Bedere, Nicolas, Burlot, Thierry, Coton, Jenna, and Le Roy, Pascale
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AGRICULTURAL egg production , *OVIPARITY , *EGGS , *GENETIC correlations , *MAXIMUM likelihood statistics , *GENETIC determinism - Abstract
In cage-free systems, laying hens must lay their eggs in the nests. Selecting layers based on nesting behavior would be a good strategy for improving egg production in these breeding systems. However, little is known about the genetic determinism of nest-related traits. Laying rate in the nests (LRN), clutch number (CN), oviposition traits (OT), and nest acceptance for laying (NAL) of 1,430 Rhode Island Red (RIR) hens and 1,008 White Leghorn (WL) hens were recorded in floor pens provided with individual electronic nests. Heritability and genetic and phenotypic correlations of all traits were estimated over two recording periods–the peak (24–43 weeks of age) and the middle (44–64 weeks of age) of production–by applying the restricted maximum likelihood method to an animal model. The mean oviposition time (MOT) ranged from 2 h 5 min to 3 h and from 3 h 35 min to 3 h 44 min after turning on the lights for RIR and WL hens, respectively. The mean oviposition interval ranged from 24 h 3 min to 24 h 16 min. All heritability and correlation estimates were similar for RIR and WL. Low to moderate heritability coefficients were estimated for LRN (0.04–0.25) and moderate to high heritability coefficients for CN and OT (0.27–0.68). CN and OT were negatively genetically correlated with LRN (-0.92 to -0.39) except during peak production for RIR (-0.30 to +0.43). NAL was weakly to moderately heritable (0.13–0.26). Genetic correlations between NAL and other traits were low to moderate (-0.41 to +0.44). In conclusion, CN and OT are promising selection criteria to improve egg production in cage-free systems. NAL can be also used to reduce the number of eggs laid off-nest in these breeding systems. However, variability in MOT must be maintained to limit competition for the nests. [ABSTRACT FROM AUTHOR]
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- 2021
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304. Interest of using imputation for genomic evaluation in layer chicken.
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Herry, Florian, Druet, David Picard, Hérault, Frédéric, Varenne, Amandine, Burlot, Thierry, Le Roy, Pascale, and Allais, Sophie
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SINGLE nucleotide polymorphisms , *LINKAGE disequilibrium , *BROILER chickens , *CHICKENS , *RANK correlation (Statistics) - Abstract
With the availability of the 600K Affymetrix Axiom high-density (HD) single nucleotide polymorphism (SNP) chip, genomic selection has been implemented in broiler and layer chicken. However, the cost of this SNP chip is too high to genotype all selection candidates. A solution is to develop a low-density SNP chip, at a lower price, and to impute all missing markers. But to routinely implement this solution, the impact of imputation on genomic evaluation accuracy must be studied. It is also interesting to study the consequences of the use of low-density SNP chips in genomic evaluation accuracy. In this perspective, the interest of using imputation in genomic selection was studied in a pure layer line. Two low-density SNP chip designs were compared: an equidistant methodology and a methodology based on linkage disequilibrium. Egg weight, egg shell color, egg shell strength, and albumen height were evaluated with single-step genomic best linear unbiased prediction methodology. The impact of imputation errors or the absence of imputation on the ranking of the male selection candidates was assessed with a genomic evaluation based on ancestry. Thus, genomic estimated breeding values (GEBV) obtained with imputed HD genotypes or low-density genotypes were compared with GEBV obtained with the HD SNP chip. The relative accuracy of GEBV was also investigated by considering as reference GEBV estimated on the offspring. A limited reordering of the breeders, selected on a multitrait index, was observed. Spearman correlations between GEBV on HD genotypes and GEBV on low-density genotypes (with or without imputation) were always higher than 0.94 with more than 3K SNP. For the genetically closer, top 150 individuals for a specific trait, with imputation, the reordering was reduced with correlation higher than 0.94 with more than 3K SNP. Without imputation, the correlations remained lower than 0.85 with less than 3K and 16K SNP for equidistant and linkage disequilibrium methodology, respectively. The differences in GEBV correlations between both methodologies were never significant. The conclusions were the same for all studied traits. [ABSTRACT FROM AUTHOR]
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- 2020
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305. Reliability of genomic evaluation for egg quality traits in layers.
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Picard Druet, David, Varenne, Amandine, Herry, Florian, Hérault, Frédéric, Allais, Sophie, Burlot, Thierry, and Le Roy, Pascale
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EGG quality , *BIRD eggs , *POULTRY breeding , *COLLATERAL circulation , *DAIRY cattle breeding , *FISH breeding , *BIRD breeding , *GENETIC markers - Abstract
Background: Genomic evaluation, based on the use of thousands of genetic markers in addition to pedigree and phenotype information, has become the standard evaluation methodology in dairy cattle breeding programmes over the past several years. Despite the many differences between dairy cattle breeding and poultry breeding, genomic selection seems very promising for the avian sector, and studies are currently being conducted to optimize avian selection schemes. In this optimization perspective, one of the key parameters is to properly predict the accuracy of genomic evaluation in pure line layers. Results: It was observed that genomic evaluation, whether performed on males or females, always proved more accurate than genetic evaluation. The gain was higher when phenotypic information was narrowed, and an augmentation of the size of the reference population led to an increase in accuracy prediction with regard to genomic evaluation. By taking into account the increase of selection intensity and the decrease of the generation interval induced by genomic selection, the expected annual genetic gain would be higher with ancestry-based genomic evaluation of male candidates than with genetic evaluation based on collaterals. This advantage of genomic selection over genetic selection requires more detailed further study for female candidates. Conclusions: In conclusion, in the population studied, the genomic evaluation of egg quality traits of breeding birds at birth seems to be a promising strategy, at least for the selection of males. [ABSTRACT FROM AUTHOR]
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- 2020
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306. Combined GWAS and LDLA approaches to improve genome-wide quantitative trait loci detection affecting carcass and meat quality traits in pig.
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Hérault, Frédéric, Damon, Marie, Cherel, Pierre, and Le Roy, Pascale
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MEAT quality , *SWINE carcasses , *BIOINFORMATICS , *MUSCLE growth , *COMPOSITION of pork - Abstract
Many QTL affecting meat quality and carcass traits have been reported. However, in most of the cases these QTL have been detected in non-commercial populations. Therefore, a family structured population of 457 F2 pigs issued from an inter-cross between 2 commercial sire lines was used to detect QTL affecting meat quality and carcass traits. All animals were genotyped using the Illumina PorcineSNP60 BeadChip platform. Genome-wide association studies were used in combination with linkage disequilibrium-linkage analysis to identify QTL. A total of 32 QTL were detected. Nine of these QTL exceeded the genome-wide 5% significance threshold. We detected 18 QTL affecting carcass composition traits and 16 QTL affecting meat quality traits. Using post-QTL bioinformatics analysis we highlighted 26 functional candidate genes related to fatness, muscle development, meat color and meat pH. Finally, our results shed light on the advantage of using different QTL detection methodologies to get a global overview of the QTL present in the studied population. [ABSTRACT FROM AUTHOR]
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- 2018
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307. Quantitative Trait Locus Analysis in Crosses Between Outbred Lines With Dominance and Inbreeding.
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Perez-Enciso, Miguel, Fernando, Rohan L., Bidanel, Jean-Pierre, and Le Roy, Pascale
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SWINE breeding , *INBREEDING - Abstract
Describes a theoretical framework for quantitative trait locus (QTL) analysis of a crossed pig population where parental lines may be outbred and dominance and inbreeding are allowed for. Assumption of a biallelic QTL; Possibility of differences in QTL allele frequencies in each breed; Expression of genetic covariance; Determination of the probabilities of each identity mode.
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- 2001
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308. Numéro spécial 'Amélioration génétique'. Avant-propos
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Mulsant, Philippe, Bodin, Loys, Coudurier, Bernard, Deretz, Severine, Le Roy, Pascale, Quillet, Edwige, and Perez, Jean-Marc
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amélioration génétique ,sélection génique ,filière avicole ,ruminant ,évaluation génétique ,élevage ,filière porcine ,séquençage du génome ,bovin laitier ,sélection animale ,phénotypage ,sélection génomique ,programme de sélection ,filière agroalimentaire ,sélection assistée par marqueurs - Published
- 2011
309. Multidimensionnalité pour la détection de gènes influençant des caractères quantitatifs. Application à l'espèce porcine
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Gilbert, Hélène, Station de Génétique Quantitative et Appliquée (SGQA), Institut National de la Recherche Agronomique (INRA), Institut National Agronomique, Inconnu Inconnu, INAPG (AgroParisTech), and Le Roy Pascale
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maximum de vraisemblance ,QTL ,multilocus ,QTL DETECTION ,MAXIMUM LIKELIHOOD ,[SDV]Life Sciences [q-bio] ,multicaractère ,[SDV.BDLR]Life Sciences [q-bio]/Reproductive Biology ,PIGS ,SIB FAMILIES ,familles ,FRATRIES ,SIMULATIONS - Abstract
Ce travail a pour but de développer des méthodes de détection de locus affectant les caractères quantitatifs, appelés QTL, à partir de l'information disponible sur des caractères corrélés et/ou des positions liées, chez les animaux d'élevage.Les méthodologies ont été dans un premier temps caractérisées pour leurs puissances et leurs précisions d'estimation des paramètres (positions et effets des QTL) à partir de données simulées. Nous avons développé d'une part des méthodes multivariées, extrapolées de techniques décrites pour l'analyse de données issues de croisements entre populations supposées génétiquement fixées, et d'autre part des méthodes synthétiques univariées, développées à l'occasion de ce travail. Ces dernières méthodes permettent de synthétiser l'information due à la présence du (des) QTL déterminant plusieurs caractères dans une unique variable, combinaison linéaire des caractères. Le nombre de paramètres à estimer est ainsi indépendant du nombre de caractères étudiés, permettant de réduire fortement les temps de calcul par rapport aux méthodes multivariées. La stratégie retenue repose sur des techniques d'analyse discriminante. Pour chaque vecteur de positions testé, des groupes de descendants sont créés en fonction de la probabilité que les individus aient reçu l'un ou l'autre haplotype de leur père. Les matrices de (co)variance génétique et résiduelle spécifiques de la présence du (des) QTL peuvent alors être estimées. La transformation linéaire permet de maximiser le rapport de ces deux variabilités.Les méthodes basées sur l'analyse de variables synthétiques permettent en général d'obtenir des résultats équivalents, voire meilleurs, que les stratégies multivariées. Seule l'estimation des effets des QTL et de la corrélation résiduelle entre les caractères reste inaccessible par ces méthodes. Une stratégie itérative basée sur l'analyse de variables synthétiques pour la sélection des caractères et des régions chromosomiques à analyser par les méthodes multivariées est proposée. Par ailleurs, nous avons quantité les apports des méthodologies multidimensionnelles pour la cartographie des QTL par rapport aux méthodes unidimensionnelles. Dans la majorité des cas, la puissance et la précision d'estimation des paramètres sont nettement améliorées. De plus, nous avons pu montrer qu'un QTL pléiotrope peut être discriminé de deux QTL liés, s'ils sont relativement distants.Ces méthodologies ont été appliquées à la détection de QTL déterminant cinq caractères de composition corporelle chez le porc sur le chromosome 7. Deux groupes de QTL déterminant des types de gras différents, le gras interne et le gras externe, ont ainsi été discriminés. Pour chacun de ces groupes, les analyses multiQTL ont permis d'identifier au moins deux régions chromosomiques distinctes déterminant les caractères.
- Published
- 2003
310. Nest preference and laying duration traits to select against floor eggs in laying hens.
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Bécot L, Bédère N, Coton J, Burlot T, and Le Roy P
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- Animals, Female, Housing, Animal, Oviposition genetics, Eggs, Chickens genetics, Animal Husbandry methods
- Abstract
Background: Floor eggs, which are defined as eggs that hens lay off-nest, are a major issue in cage-free layer poultry systems. They create additional work for farmers because they must be collected by hand. They are also usually soiled or broken, which results in economic losses. Nonetheless, knowledge about the genetics of nesting behavior is limited. The aim of this study was to estimate genetic parameters for traits related to nest preference for laying and to time spent in the nests used for laying (laying duration)., Methods: Two pure lines of laying hens were studied: 927 Rhode Island Red and 980 White Leghorn. Electronic nests were used to record the nesting behavior of these hens in floor pens from 24 to 64 weeks of age. Nest preference was studied based on the mean distance between nests used for laying and the percentage of nests used for laying. Laying duration was studied based on mean laying duration, mean duration in the nest before laying, and mean duration in the nest after laying. Genetic parameters were estimated for each line using a restricted maximum-likelihood method applied to a pedigree-based multi-trait animal model., Results: Estimates of genetic parameters were similar for the two lines. Estimates of heritability ranged from 0.18 to 0.37 for nest preference traits and from 0.54 to 0.70 for laying duration traits. Estimates of genetic correlations of these traits with clutch number or mean oviposition time were favorable. Positive genetic correlations were estimated between nest preference and laying rate in the nests or nest acceptance for laying (+ 0.06 to + 0.37)., Conclusions: These results show that genetics influences traits related to nest preference and laying duration. Selecting hens that have no preference for particular nests and spend little time laying in the nests could help optimize nest use, reduce their occupation rate, and thus decrease the incidence of floor eggs in cage-free systems. Genetic correlations of these traits with other traits of interest related to hen welfare and egg quality have yet to be estimated., (© 2023. The Author(s).)
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- 2023
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311. GWAS analyses reveal QTL in egg layers that differ in response to diet differences.
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Romé H, Varenne A, Hérault F, Chapuis H, Alleno C, Dehais P, Vignal A, Burlot T, and Le Roy P
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- Animals, Chickens genetics, Chromosome Mapping, Diet, Female, Gene-Environment Interaction, Genome-Wide Association Study, Polymorphism, Single Nucleotide, Chickens physiology, Oviparity, Quantitative Trait Loci
- Abstract
Background: The genetic architecture of egg production and egg quality traits, i.e. the quantitative trait loci (QTL) that influence these traits, is still poorly known. To date, 33 studies have focused on the detection of QTL for laying traits in chickens, but less than 10 genes have been identified. The availability of a high-density SNP (single nucleotide polymorphism) chicken array developed by Affymetrix, i.e. the 600K Affymetrix(®) Axiom(®) HD genotyping array offers the possibility to narrow down the localization of previously detected QTL and to detect new QTL. This high-density array is also anticipated to take research beyond the classical hypothesis of additivity of QTL effects or of QTL and environmental effects. The aim of our study was to search for QTL that influence laying traits using the 600K SNP chip and to investigate whether the effects of these QTL differed between diets and age at egg collection., Results: One hundred and thirty-one QTL were detected for 16 laying traits and were spread across all marked chromosomes, except chromosomes 16 and 25. The percentage of variance explained by a QTL varied from 2 to 10 % for the various traits, depending on diet and age at egg collection. Chromosomes 3, 9, 10 and Z were overrepresented, with more than eight QTL on each one. Among the 131 QTL, 60 had a significantly different effect, depending on diet or age at egg collection. For egg production traits, when the QTL × environment interaction was significant, numerous inversions of sign of the SNP effects were observed, whereas for egg quality traits, the QTL × environment interaction was mostly due to a difference of magnitude of the SNP effects., Conclusions: Our results show that numerous QTL influence egg production and egg quality traits and that the genomic regions, which are involved in shaping the ability of layer chickens to adapt to their environment for egg production, vary depending on the environmental conditions. The next question will be to address what the impact of these genotype × environment interactions is on selection.
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- 2015
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312. The Longissimus and Semimembranosus muscles display marked differences in their gene expression profiles in pig.
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Herault F, Vincent A, Dameron O, Le Roy P, Cherel P, and Damon M
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- Animals, Gene Expression Profiling, Meat, Sus scrofa metabolism, Swine, Tissue Array Analysis, Transcriptome, Muscle, Skeletal metabolism, Sus scrofa genetics
- Abstract
Background: Meat quality depends on skeletal muscle structure and metabolic properties. While most studies carried on pigs focus on the Longissimus muscle (LM) for fresh meat consumption, Semimembranosus (SM) is also of interest because of its importance for cooked ham production. Even if both muscles are classified as glycolytic muscles, they exhibit dissimilar myofiber composition and metabolic characteristics. The comparison of LM and SM transcriptome profiles undertaken in this study may thus clarify the biological events underlying their phenotypic differences which might influence several meat quality traits., Methodology/principal Findings: Muscular transcriptome analyses were performed using a custom pig muscle microarray: the 15 K Genmascqchip. A total of 3823 genes were differentially expressed between the two muscles (Benjamini-Hochberg adjusted P value ≤0.05), out of which 1690 and 2133 were overrepresented in LM and SM respectively. The microarray data were validated using the expression level of seven differentially expressed genes quantified by real-time RT-PCR. A set of 1047 differentially expressed genes with a muscle fold change ratio above 1.5 was used for functional characterization. Functional annotation emphasized five main clusters associated to transcriptome muscle differences. These five clusters were related to energy metabolism, cell cycle, gene expression, anatomical structure development and signal transduction/immune response., Conclusions/significance: This study revealed strong transcriptome differences between LM and SM. These results suggest that skeletal muscle discrepancies might arise essentially from different post-natal myogenic activities.
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- 2014
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313. QTL detection for coccidiosis (Eimeria tenella) resistance in a Fayoumi × Leghorn F₂ cross, using a medium-density SNP panel.
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Bacciu N, Bed'Hom B, Filangi O, Romé H, Gourichon D, Répérant JM, Le Roy P, Pinard-van der Laan MH, and Demeure O
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- Animals, Coccidiosis genetics, Crosses, Genetic, Genetic Variation, Genotype, Polymorphism, Single Nucleotide, Quantitative Trait Loci, Chickens genetics, Chickens parasitology, Coccidiosis veterinary, Eimeria tenella isolation & purification, Poultry Diseases genetics, Poultry Diseases parasitology
- Abstract
Background: Coccidiosis is a major parasitic disease that causes huge economic losses to the poultry industry. Its pathogenicity leads to depression of body weight gain, lesions and, in the most serious cases, death in affected animals. Genetic variability for resistance to coccidiosis in the chicken has been demonstrated and if this natural resistance could be exploited, it would reduce the costs of the disease. Previously, a design to characterize the genetic regulation of Eimeria tenella resistance was set up in a Fayoumi × Leghorn F2 cross. The 860 F2 animals of this design were phenotyped for weight gain, plasma coloration, hematocrit level, intestinal lesion score and body temperature. In the work reported here, the 860 animals were genotyped for a panel of 1393 (157 microsatellites and 1236 single nucleotide polymorphism (SNP) markers that cover the sequenced genome (i.e. the 28 first autosomes and the Z chromosome). In addition, with the aim of finding an index capable of explaining a large amount of the variance associated with resistance to coccidiosis, a composite factor was derived by combining the variables of all these traits in a single variable. QTL detection was performed by linkage analysis using GridQTL and QTLMap. Single and multi-QTL models were applied., Results: Thirty-one QTL were identified i.e. 27 with the single-QTL model and four with the multi-QTL model and the average confidence interval was 5.9 cM. Only a few QTL were common with the previous study that used the same design but focused on the 260 more extreme animals that were genotyped with the 157 microsatellites only. Major differences were also found between results obtained with QTLMap and GridQTL., Conclusions: The medium-density SNP panel made it possible to genotype new regions of the chicken genome (including micro-chromosomes) that were involved in the genetic control of the traits investigated. This study also highlights the strong variations in QTL detection between different models and marker densities.
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- 2014
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314. Statistical properties of interval mapping methods on quantitative trait loci location: impact on QTL/eQTL analyses.
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Wang X, Gilbert H, Moreno C, Filangi O, Elsen JM, and Le Roy P
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- Algorithms, Computer Simulation, Genetic Linkage, Genetics, Population methods, Humans, Models, Genetic, Phenotype, Regression Analysis, Software, Chromosome Mapping, Quantitative Trait Loci genetics, Transcriptome genetics
- Abstract
Background: Quantitative trait loci (QTL) detection on a huge amount of phenotypes, like eQTL detection on transcriptomic data, can be dramatically impaired by the statistical properties of interval mapping methods. One of these major outcomes is the high number of QTL detected at marker locations. The present study aims at identifying and specifying the sources of this bias, in particular in the case of analysis of data issued from outbred populations. Analytical developments were carried out in a backcross situation in order to specify the bias and to propose an algorithm to control it. The outbred population context was studied through simulated data sets in a wide range of situations.The likelihood ratio test was firstly analyzed under the "one QTL" hypothesis in a backcross population. Designs of sib families were then simulated and analyzed using the QTL Map software. On the basis of the theoretical results in backcross, parameters such as the population size, the density of the genetic map, the QTL effect and the true location of the QTL, were taken into account under the "no QTL" and the "one QTL" hypotheses. A combination of two non parametric tests - the Kolmogorov-Smirnov test and the Mann-Whitney-Wilcoxon test - was used in order to identify the parameters that affected the bias and to specify how much they influenced the estimation of QTL location., Results: A theoretical expression of the bias of the estimated QTL location was obtained for a backcross type population. We demonstrated a common source of bias under the "no QTL" and the "one QTL" hypotheses and qualified the possible influence of several parameters. Simulation studies confirmed that the bias exists in outbred populations under both the hypotheses of "no QTL" and "one QTL" on a linkage group. The QTL location was systematically closer to marker locations than expected, particularly in the case of low QTL effect, small population size or low density of markers, i.e. designs with low power. Practical recommendations for experimental designs for QTL detection in outbred populations are given on the basis of this bias quantification. Furthermore, an original algorithm is proposed to adjust the location of a QTL, obtained with interval mapping, which co located with a marker., Conclusions: Therefore, one should be attentive when one QTL is mapped at the location of one marker, especially under low power conditions.
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- 2012
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315. Complex trait subtypes identification using transcriptome profiling reveals an interaction between two QTL affecting adiposity in chicken.
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Blum Y, Le Mignon G, Causeur D, Filangi O, Désert C, Demeure O, Le Roy P, and Lagarrigue S
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- Animals, Male, Adiposity genetics, Chickens genetics, Gene Expression Profiling, Quantitative Trait Loci, Transcriptome
- Abstract
Background: Integrative genomics approaches that combine genotyping and transcriptome profiling in segregating populations have been developed to dissect complex traits. The most common approach is to identify genes whose eQTL colocalize with QTL of interest, providing new functional hypothesis about the causative mutation. Another approach includes defining subtypes for a complex trait using transcriptome profiles and then performing QTL mapping using some of these subtypes. This approach can refine some QTL and reveal new ones.In this paper we introduce Factor Analysis for Multiple Testing (FAMT) to define subtypes more accurately and reveal interaction between QTL affecting the same trait. The data used concern hepatic transcriptome profiles for 45 half sib male chicken of a sire known to be heterozygous for a QTL affecting abdominal fatness (AF) on chromosome 5 distal region around 168 cM., Results: Using this methodology which accounts for hidden dependence structure among phenotypes, we identified 688 genes that are significantly correlated to the AF trait and we distinguished 5 subtypes for AF trait, which are not observed with gene lists obtained by classical approaches. After exclusion of one of the two lean bird subtypes, linkage analysis revealed a previously undetected QTL on chromosome 5 around 100 cM. Interestingly, the animals of this subtype presented the same q paternal haplotype at the 168 cM QTL. This result strongly suggests that the two QTL are in interaction. In other words, the "q configuration" at the 168 cM QTL could hide the QTL existence in the proximal region at 100 cM. We further show that the proximal QTL interacts with the previous one detected on the chromosome 5 distal region., Conclusion: Our results demonstrate that stratifying genetic population by molecular phenotypes followed by QTL analysis on various subtypes can lead to identification of novel and interacting QTL.
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- 2011
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316. Genetic variability of transcript abundance in pig peri-mortem skeletal muscle: eQTL localized genes involved in stress response, cell death, muscle disorders and metabolism.
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Liaubet L, Lobjois V, Faraut T, Tircazes A, Benne F, Iannuccelli N, Pires J, Glénisson J, Robic A, Le Roy P, Sancristobal M, and Cherel P
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- Animals, Cell Death genetics, Chromosome Mapping, Cluster Analysis, Female, Gene Expression Regulation, Genetic Variation, Male, Molecular Sequence Annotation, Oligonucleotide Array Sequence Analysis, Phenotype, Stress, Physiological genetics, Swine metabolism, Transcription, Genetic, Muscle, Skeletal metabolism, Quantitative Trait Loci, Swine genetics, Transcriptome
- Abstract
Background: The genetics of transcript-level variation is an exciting field that has recently given rise to many studies. Genetical genomics studies have mainly focused on cell lines, blood cells or adipose tissues, from human clinical samples or mice inbred lines. Few eQTL studies have focused on animal tissues sampled from outbred populations to reflect natural genetic variation of gene expression levels in animals. In this work, we analyzed gene expression in a whole tissue, pig skeletal muscle sampled from individuals from a half sib F2 family shortly after slaughtering., Results: QTL detection on transcriptome measurements was performed on a family structured population. The analysis identified 335 eQTLs affecting the expression of 272 transcripts. The ontologic annotation of these eQTLs revealed an over-representation of genes encoding proteins involved in processes that are expected to be induced during muscle development and metabolism, cell morphology, assembly and organization and also in stress response and apoptosis. A gene functional network approach was used to evidence existing biological relationships between all the genes whose expression levels are influenced by eQTLs. eQTLs localization revealed a significant clustered organization of about half the genes located on segments of chromosome 1, 2, 10, 13, 16, and 18. Finally, the combined expression and genetic approaches pointed to putative cis-drivers of gene expression programs in skeletal muscle as COQ4 (SSC1), LOC100513192 (SSC18) where both the gene transcription unit and the eQTL affecting its expression level were shown to be localized in the same genomic region. This suggests cis-causing genetic polymorphims affecting gene expression levels, with (e.g. COQ4) or without (e.g. LOC100513192) potential pleiotropic effects that affect the expression of other genes (cluster of trans-eQTLs)., Conclusion: Genetic analysis of transcription levels revealed dependence among molecular phenotypes as being affected by variation at the same loci. We observed the genetic variation of molecular phenotypes in a specific situation of cellular stress thus contributing to a better description of muscle physiologic response. In turn, this suggests that large amounts of genetic variation, mediated through transcriptional networks, can drive transient cell response phenotypes and contribute to organismal adaptative potential.
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- 2011
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317. Identification of QTL with effects on intramuscular fat content and fatty acid composition in a Duroc x Large White cross.
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Sanchez MP, Iannuccelli N, Basso B, Bidanel JP, Billon Y, Gandemer G, Gilbert H, Larzul C, Legault C, Riquet J, Milan D, and Le Roy P
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- Animals, Crosses, Genetic, Female, Genetic Markers, Male, Dietary Fats analysis, Fatty Acids analysis, Meat analysis, Muscles chemistry, Quantitative Trait Loci, Sus scrofa genetics
- Abstract
Background: Improving pork quality can be done by increasing intramuscular fat (IMF) content. This trait is influenced by quantitative trait loci (QTL) sought out in different pig populations. Considering the high IMF content observed in the Duroc pig, it was appealing to determine whether favourable alleles at a major gene or QTL could be found. The detection was performed in an experimental F2 Duroc x Large White population first by segregation analysis, then by QTL mapping using additional molecular information., Results: Segregation analysis provided evidence for a major gene, with a recessive Duroc allele increasing IMF by 1.8% in Duroc homozygous pigs. However, results depended on whether data were normalised or not. After Box-Cox transformation, likelihood ratio was indeed 12 times lower and no longer significant. The QTL detection results were partly consistent with the segregation analysis. Three QTL significant at the chromosome wide level were evidenced. Two QTL, located on chromosomes 13 and 15, showed a high IMF Duroc recessive allele with an overall effect slightly lower than that expected from segregation analysis (+0.4 g/100 g muscle). The third QTL was located on chromosome 1, with a dominant Large White allele inducing high IMF content (+0.5 g/100 g muscle). Additional QTL were detected for muscular fatty acid composition., Conclusion: The study presented results from two complementary approaches, a segregation analysis and a QTL detection, to seek out genes involved in the higher IMF content observed in the Duroc population. Discrepancies between both methods might be partially explained by the existence of at least two QTL with similar characteristics located on two different chromosomes for which different boars were heterozygous. The favourable and dominant allele detected in the Large White population was unexpected. Obviously, in both populations, the favourable alleles inducing high IMF content were not fixed and improving IMF by fixing favourable alleles using markers can then be applied both in Duroc and LW populations. With QTL affecting fatty acid composition, combining an increase of IMF content enhancing monounsaturated fatty acid percentage would be of great interest.
- Published
- 2007
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318. Detection of novel quantitative trait loci for cutaneous melanoma by genome-wide scan in the MeLiM swine model.
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Du ZQ, Vincent-Naulleau S, Gilbert H, Vignoles F, Créchet F, Shimogiri T, Yasue H, Leplat JJ, Bouet S, Gruand J, Horak V, Milan D, Le Roy P, and Geffrotin C
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- Alleles, Animals, Chromosome Mapping, Female, Genetic Predisposition to Disease, Humans, Male, Phylogeny, Receptor, Melanocortin, Type 1 genetics, Swine classification, Swine, Miniature classification, Disease Models, Animal, Melanoma genetics, Quantitative Trait Loci, Skin Neoplasms genetics, Swine genetics, Swine, Miniature genetics
- Abstract
Human cutaneous melanoma is a complex trait inherited in about 10% of cases. Although 2 high-risk genes, CDKN2A and CDK4, and 1 low risk gene, MC1R, have been identified, susceptibility genes remain to be discovered. Here, we attempted to determine new genomic regions linked to melanoma using the pig MeLiM strain, which develops hereditary cutaneous melanomas. We applied quantitative trait loci (QTL) mapping method to a significant genome-wide scan performed on 331 backcross pigs derived from this strain. QTLs were detected at chromosome-wide level for a melanoma synthetic trait corresponding to the development of melanoma. The peak positions on Sus scrofa chromosomes (SSC) were at 49.4 and 88.0 cM (SSC1), 56.0 cM (SSC13), 86.5 cM (SSC15) and 39.8 cM (SSC17), and, on SSC2, at 16.9 cM, in families derived from F1 males only (p < 0.05, except for SSC13, p < 0.01). Analysis of 7 precise specific traits revealed highly significant QTLs on SSC10 (ulceration), on SSC12 (presence of melanoma at birth), on SSC13 (lesion type), and on SSC16 and SSC17 (number of aggressive melanomas) at the respective positions 42.0, 95.6, 81.0, 45.3 and 44.8 cM (p < 0.001 and p < 0.05 respectively at the chromosome- and genome-wide levels). We also showed that MeLiM MC1R*2 allele, which determines black coat colour in pigs, predisposes significantly to melanoma. Interactions were observed between MC1R and markers located on SSC1 (p < 0.05). Taken together, these results indicate that MeLiM swine is a model for human multigenic diseases. Comparative mapping revealed human regions of interest to search for new melanoma susceptibility candidates., ((c) 2006 Wiley-Liss, Inc.)
- Published
- 2007
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