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In this study, polyethylene glycol (PEG) with hydroxyapatite (HA), with the incorporation of physical gold nanoparticles (AuNPs), was created and equipped through a surface coating technique in order to form PEG-HA-AuNP nanocomposites. The surface morphology and chemical composition were characterized using scanning electron microscopy (SEM), atomic force microscopy (AFM), UV–Vis spectroscopy (UV–Vis), Fourier transform infrared spectroscopy (FTIR), X-ray photoelectron spectroscopy (XPS), and contact angle assessment. The effects of PEG-HA-AuNP nanocomposites on the biocompatibility and biological activity of MC3T3-E1 osteoblast cells, endothelial cells (EC), macrophages (RAW 264.7), and human mesenchymal stem cells (MSCs), as well as the guiding of osteogenic differentiation, were estimated through the use of an in vitro assay. Moreover, the anti-inflammatory, biocompatibility, and endothelialization capacities were further assessed through in vivo evaluation. The PEG-HA-AuNP nanocomposites showed superior biological properties and biocompatibility capacity for cell behavior in both MC3T3-E1 cells and MSCs. These biological events surrounding the cells could be associated with the activation of adhesion, proliferation, migration, and differentiation processes on the PEG-HA-AuNP nanocomposites. Indeed, the induction of the osteogenic differentiation of MSCs by PEG-HA-AuNP nanocomposites and enhanced mineralization activity were also evidenced in this study. Moreover, from the in vivo assay, we further found that PEG-HA-AuNP nanocomposites not only facilitate the anti-immune response, as well as reducing CD86 expression, but also facilitate the endothelialization ability, as well as promoting CD31 expression, when implanted into rats subcutaneously for a period of 1 month. The current research illustrates the potential of PEG-HA-AuNP nanocomposites when used in combination with MSCs for the regeneration of bone tissue, with their nanotopography being employed as an applicable surface modification approach for the fabrication of biomaterials.

A TiCnp /Mg–2Zn-0.8Sr-0.2Ca magnesium matrix composite was processed by the combination of multidirectional forging (MDF) with extrusion (EX). The composite exhibited ultrahigh mechanical properties (yield strength of ~500 MPa and ultimate tensile strength of ~544 MPa) after 6 passes of MDF at 300 °C, followed by EX at 0.1 mm/s and 200 °C. The grain boundary strengthening effect caused by grain refinement and high recrystallization rate, dislocation strengthening effect originated from the severe plastic deformation , as well as the Orowan strengthening effect inspired by the dynamically precipitated MgZn 2 phases and the added TiCnp were considered as the main contributors to the substantial increase in strength.

The engineering of vascular regeneration still involves barriers that need to be conquered. In the current study, a novel nanocomposite comprising of fibronectin (denoted as FN) and a small amount of silver nanoparticles (AgNP, ~15.1, ~30.2 or ~75.5 ppm) was developed and its biological function and biocompatibility in Wharton’s jelly-derived mesenchymal stem cells (MSCs) and rat models was investigated. The surface morphology as well as chemical composition for pure FN and the FN-AgNP nanocomposites incorporating various amounts of AgNP were firstly characterized by atomic force microscopy (AFM), UV-Visible spectroscopy (UV-Vis), and Fourier-transform infrared spectroscopy (FTIR). Among the nanocomposites, FN-AgNP with 30.2 ppm silver nanoparticles demonstrated the best biocompatibility as assessed through intracellular ROS production, proliferation of MSCs, and monocytes activation. The expression levels of pro-inflammatory cytokines, TNF-α, IL-1β, and IL-6, were also examined. FN-AgNP 30.2 ppm significantly inhibited pro-inflammatory cytokine expression compared to other materials, indicating superior performance of anti-immune response. Mechanistically, FN-AgNP 30.2 ppm significantly induced greater expression of vascular endothelial growth factor (VEGF) and stromal-cell derived factor-1 alpha (SDF-1α) and promoted the migration of MSCs through matrix metalloproteinase (MMP) signaling pathway. Besides, in vitro and in vivo studies indicated that FN-AgNP 30.2 ppm stimulated greater protein expressions of CD31 and von Willebrand Factor (vWF) as well as facilitated better endothelialization capacity than other materials. Furthermore, the histological tissue examination revealed the lowest capsule formation and collagen deposition in rat subcutaneous implantation of FN-AgNP 30.2 ppm. In conclusion, FN-AgNP nanocomposites may facilitate the migration and proliferation of MSCs, induce endothelial cell differentiation, and attenuate immune response. These finding also suggests that FN-AgNP may be a potential anti-inflammatory surface modification strategy for vascular biomaterials.

Inspired by the intriguing structures and bioactivities of dimeric alkaloids, 11 new thalifaberine-type aporphine-benzylisoquinoline alkaloids, thalicultratines A-K, a tetrahydroprotoberberine-aporphine alkaloid, thalicultratine L, and five known ones were isolated from the roots of Thalictrum cultratum. Their structures were defined on the basis of NMR and HRESIMS data. The antiproliferative activities of compounds 1-17 were evaluated against human leukemia HL-60 and prostate cancer PC-3 cells. Most alkaloids showed potent cytotoxicity against selected cancer cells. Preliminary SARs are discussed. The most active new compound (3), with an IC50 value of 1.06 μM against HL-60 cells, was selected for mechanism of action studies. The results revealed that compound 3 induced apoptosis and arrested the HL-60 cell cycle at the S phase with the loss of mitochondria membrane potential. The nuclear morphological Hoechst 33258 staining assay was also carried out, and the results confirmed apoptosis.

The Niujuan breccia-type silver deposit forms part of the North Hebei metallogenic belt along the northern margin of the North China Craton. The Hercynian Baiyingou coarse-grained granite and the Yanshanian Er’daogou fine-grained granite are the major Mesozoic intrusions exposed in this region. Here we investigate the salient characteristics of the mineralization and evaluate its genesis through zircon U-Pb and fluorite Sm-Nd age data, and Pb, S, O, H, He and Ar isotope data. The orebodies of the Niujuan silver deposit are hosted in breccias, which contain angular fragments of the Baiyingou and Er’daogou granitoids. The δ34S values of pyrite from the silver mineralized veins range from 2.4‰ to 5.3‰. The 206Pb/204Pb, 207Pb/204Pb and 208Pb/204Pb ratios of the sulfide minerals show ranges of 16.837–16.932, 15.420–15.501 and 37.599–37.950, respectively. The 3He/4He and 40Ar/36Ar ratios of the fluids trapped in pyrite are 0.921–4.81Ra and 299.34–303.84, respectively. The δ18O and δ18Dw values of the ore-forming fluids range from 0.6‰ to −4.15‰ and from −119.4‰ to −98.7‰, respectively. Our isotopic data suggest that the ore-forming fluids were originally derived from the subvolcanic plutons and evolved into a mixture of magmatic and meteoric water during the main hydrothermal stage. The ore-forming materials were primarily derived from the lower crust with limited incorporation of mantle materials. The emplacement time of the Er’daogou granite is constrained by LA-ICP-MS zircon U-Pb geochronology at 145.5 ± 2.1 Ma. Five fluorite samples from the last hydrothermal stage yielded a Sm-Nd isochron age of 139.2 ± 3.8 Ma, indicating the upper age limit for the silver mineralization. These ages correlate with the formation of the Niujuan deposit in an extensional setting associated with the closure of the Mongol-Okhotsk Ocean and the subduction of the Paleo-Pacific oceanic plate beneath the North China Craton.

The Mg-4Zn-0.5Ca alloy was processed by the combination of forging, homogenization and extrusion in present study. The results showed that homogenization treatment between forging and extrusion process can release stored energy and diminish precipitated MgZn 2 phase, both of which result in the finer size and low volume fraction of dynamic recrystallized (DRXed) grains, as well as strong basal plane texture in the as-extruded alloy (FD-H-ED). As compared with the as-extruded alloy without homogenization treatment (FD-ED), the FD-H-ED alloy exhibited high yield strength which is thought to originate from the dislocation strengthening and texture strengthening. Only stage III and stage IV appeared in the θ vs. ( σ − σ 0.2 ) curves of FD-ED and FD-H-ED alloys. Large amount of fine DRXed grains in FD-ED alloy led to the high dynamic recovery rate at stage III. However, the unDRXed regions in FD-H-ED alloy resulted in its unobvious starting point and promote the decrease of θ value at Stage Ⅳ.

Mg–3Al–Zn alloy with the addition of Al and Si as a eutectic alloy was subjected to conventional hot rolling. The corresponding mechanical properties, microstructure evolution, and dynamic recrystallization mechanism were investigated by optical microscope, scanning electron microscope, electron backscattered diffraction (EBSD), and tensile tests. The experimental results indicated that the Mg–3(Al–Si)–Zn alloy had a microstructure refinement, thus rendering an enhanced mechanical properties in comparison with the Mg–3Al–Zn alloy. The refined Mg2Si particles could act as potential nucleation sites for recrystallization in as-rolled Mg–3(Al–Si)–Zn alloy sheets, which resulted in more completely recrystallized regions through particle stimulated nucleation and a weakened basal texture compared to Mg–3Al–Zn alloy. The improvement in the tensile strength of the as-rolled Mg–3(Al–Si)–Zn alloy can be attributed to grain refinement and second phase strengthening caused by the refined Mg2Si particles.

DC power relay is a key electromechanical component widely used in electrical vehicles, photovoltaics, as well as more-electric aircrafts for energy managing, power conversion, and distribution. As the voltage and current increase due to relay switches, arc discharge becomes an unavoidable and significant issue during the operation of the dc high-power relay. Bridge-type contact and external permanent magnets are spontaneously employed in its arc chamber to accelerate the quenching of arc. In this paper, the complicated arc discharge plasma in a dc power relay has been investigated by using both experimental and numerical approaches. Initially, arc dwell and arc restriking characteristics during the arc motion phase are presented by analyzing the voltage and current waveforms and arc images acquired in carefully designed breaking experiments. The arc driven by a transverse magnetic field with a variety of flux densities and the influence of flux densities on arc voltage and dwell are preliminarily discussed based on the experimental results. Then, to reveal the arc physics and to determine the influence of transverse magnetic field on arc motion characteristics, a simplified model based on the magnetohydrodynamic (MHD) theory is built and three cases with different magnetic field are calculated. The simulated arc behaviors including distributions of the temperature and current density are presented. The mechanism underlying the influence of transverse magnetic field on the arc motion is analyzed according to arc root transfer and arc voltage variation. In addition, to understand the formation of the arc restriking, the critical breakdown electric-field strength inside the arc gap is also calculated based on streamer breakdown theory. The parameters in the space occupied by earlier arcs, as predicted by the MHD simulations, are used in the breakdown calculation. Results show that the critical breakdown voltage reduces remarkably along with growth of the operating voltage and breaking current, and the arc dwell time can be reduced by strengthening the external magnetic field within a reasonable extent.

SiC nanoparticles reinforced magnesium matrix composite was fabricated by ultrasonic vibration assisted squeeze casting. Since ultrasonic device could meet the use requirements according to theoretic calculation, uniform dispersion of SiC nanoparticles was expected to achieve. The grains of the composite were refined compared with the AZ91 alloy, which was related to the increase of nucleation sites during solidification and Zenner pinning effect caused by SiC nanoparticles. With increasing the ultrasonic power, grain size of the composite changed no obviously while the morphology of β-Mg17Al12 phase was significantly affected. The ultimate tensile strength, yield strength, and elongation to fracture of the composites fabricated under different ultrasonic powers were simultaneously improved compared with the AZ91 alloy. The increase of yield strength could be attributed to Hall–Petch strengthening and Orowan strengthening for the present composites. Theoretical value of the yield strength obtained by the square root method was close to the experimental value.

In this study, low volume fraction SiCp/AZ91 magnesium matrix nanocomposites billets intended for structural applications were synthesized using semisolid stirring assisted ultrasonic vibration, leading to the dispersion of SiC nanoparticles. Both the AZ91 alloy and nanocomposite billets were then subjected to isothermal multidirectional forging (IMDF). Micrographic observations illustrated that the mean grain size of the developed nanocomposite showed an initial increase after 3 IMDF passes, followed by a decrease after 6 IMDF passes compared to the AZ91 alloy. This indicated that the effect of dispersed SiC nanoparticles on the inhibition of the dynamic recrystallization grains growth was impaired due to the present high IMDF temperature. The improved yield strength of the nanocomposite could be attributed to Orowan strengthening effect related to the dispersed SiC nanoparticles as well as the second phases precipitated far from the dynamic recrystallization grains.

The SiCp/Mg-4Zn-0.5Ca composite was fabricated by semi-solid stirring assisted ultrasonic vibration and followed by ultra-slow-speed (0.01 mm s −1 ) extrusion at 280 °C. The results show that the as-extruded composite exhibits a typical bimodal microstructure consisted of ∼96.2% ultrafine dynamic recrystallization (DRXed) grains (∼0.86 μm) and relatively coarse unDRXed regions in a shape of elongated strips. Large amount of nanoscale MgZn 2 dynamically precipitated during ultra-slow-speed extrusion. The DRXed regions are thought propitious to the nucleation and growth of precipitate as compared with that of unDRXed regions. Compared with previous works on particle reinforced Mg matrix composites, the as-extruded SiCp/Mg-4Zn-0.5Ca composite not only possess high yield strength (322.7 MPa) and ultimate tensile strength (409.1 MPa), but also exhibit excellent ductility (10.1%). The ultrafine DRXed grains and large amount of dynamic precipitated nanoscale MgZn 2 are thought responsible for the superior strength of present composite. A typical linear hardening stage appears in the work hardening rate curve of as-extruded composite. Compared with the hinder effect of SiCp and dynamic precipitated nanoscale MgZn 2 on the movement of dislocations, the ultrafine DRXed grains play a main role on aggravating the dynamic recovery rate in stage Ⅲ.

Investigation of the alkaloids from Peganum harmala seeds yielded two pairs of unique racemic pyrroloindole alkaloids, (±)-peganines A–B (1–2); two rare thiazole derivatives, peganumals A–B (3–4); six new β-carboline alkaloids, pegaharmines F–K (5–10); and 12 known analogues. Their structures, including stereochemistry, were elucidated through spectroscopic analyses, quantum chemistry calculations, and single-crystal X-ray diffraction. Notably, the incorporation of pyrrole and indole moieties in peganines A–B, thiazole fragments in peganumals A–B, and a C-1 α,β-unsaturated ester motif in pegaharmine F (5) are all rare, and their presence in the genus Peganum were demonstrated for the first time. All isolates were tested for antiproliferative activities against the HL-60, PC-3, and SGC-7901 cancer cell lines, and compounds 9, 11, 12, and 13 exhibited moderate cytotoxicity against HL-60 cancer cell lines with IC50 values in the range of 4.36–9.25 μM.

This article reviews recent advances in the shape-controlled synthesis routes of hierarchical Ag dendritic nanostructures. Various fabricated techniques, with or without templates or surfactants, electrochemical method and sonochemical, microwave or photochemical assisted synthesis method, can be amazingly effective in the dendritic Ag nanostructures synthesis, if synthetic parameters are carefully selected. The involved synthesis mechanism are discussed in order to control the limited factors along the fabricated progress efficiently and then to find new synthetic means. Also, the blemish of the above synthesis routes that hinders the practical application is pointed out to inspire the future research.

The microstructure evolution of Mg100-2xYxZnx (x=2, 2.5, 3, 3.5) alloys was investigated. Results show that the Mg100-2xYxZnx alloys are composed of α-Mg, long period stacking ordered (LPSO) phase and eutectic structure phase (W phase), and the Mg95Y2.5Zn2.5 alloy has the best comprehensive mechanical properties. Subsequently, the microstructure evolution of the optimized alloy Mg95Y2.5Zn2.5 during solidification and heat treatment processes was analyzed and discussed by means of OM, SEM, TEM, XRD and DTA. After heat treatment, the lamellar phase 14H-LPSO precipitated in α-Mg and W phase transforms into particle phase (MgYZn2). Due to the compound reinforcement effect of the particle phase and LPSO phase (18R+14H), the mechanical properties of the alloy are enhanced. The tensile strength and elongation of the Mg95Y2.5Zn2.5 alloy is improved by 9.1% and 31.3% to 215 MPa and 10.5%, respectively, after solid-solution treatment.

Neobraclactones A–C (1–3), featuring an unprecedented further rearranged prenylxanthone skeleton with a unique octahydro-2H-1,3-dioxacyclopenta[c,d]inden-2-one scaffold, along with their biosynthesis-related known compound neobractatin (4), were isolated from the leaves of Garcinia bracteata. Their structures with absolute configurations were determined by extensive analyses of spectroscopic data and ECD calculations. Compounds 1 and 2 showed significant growth inhibition activities against the human leukaemia HL-60 and K562 cell lines with GI50 values from 0.40 to 0.86 μM.