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Mesenchymal stem cells (MSCs) have potential role in organ regeneration therapy. Previous work indicating that MSCs confer protection against liver disease. Here, we aimed to determine the potential application in liver regeneration of human placenta-derived MSCs extracellular vesicles (hPMSCs-EVs) via experimental hepatectomy. hPMSCs-EVs were administered intravenously 24 h before 70% partial hepatectomy, the specific composition of hPMSCs-EVs was identified by sequencing and validated by the quantitative polymerase chain reaction, including circ-RBM23. The role of circ-RBM23 in L02 cell was evaluated and it was found that circ-RBM23 knockdown inhibited L02 cell proliferation both in vitro and in vivo. The competing endogenous RNA function of circ-RBM23 was evaluated by the RNA immunoprecipitation assay and found that circ-RBM23 shares miRNA response elements with RRM2. Overexpressed circ-RBM23 bound competitively to miR-139-5p, preventing the miRNA-mediated degradation of RRM2, activating the expression of eIF4G and AKT/mTOR, and facilitating liver regeneration. These results indicate that hPMSCs-EVs prevent hepatic dysfunction and improve liver regeneration in vivo and hepatocytes proliferation in vitro, potentially via circ-RBM23 delivery.

Maize grain size is the main factor determining grain yield. Dissecting the genetic basis of maize grain size may help reveal the regulatory mechanism of maize seed development and yield formation. In this study, two associated populations were used for genome-wide association analysis of kernel length, kernel width, kernel thickness, and hundred-kernel weight from multiple locations in AM122 and AM180, respectively. Then, genome-wide association mapping was performed based on the maize 6H90K SNP chip. A total of 139 loci were identified as associated with the four traits with p < 1 × 10−4 using two models (FarmCPU and MLM). The transcriptome data showed that 15 of them were expressed differentially in two maize-inbred lines KB182 (small kernel) and KB020 (big kernel) during kernel development. These candidate genes were enriched in regulating peroxidase activity, oxidoreductase, and leaf senescence. The molecular function was major in binding and catalytic activity. This study provided important reference information for exploring maize kernel development mechanisms and applying molecular markers in high-yield breeding.

The effects of heat shock protein 70 (HSP70)/HSP70-peptide complexes (HSP70-PCs) on the invasion and metastasis ability of hepatocellular carcinoma (HCC) Huh-7 cells were investigated. Wound healing assay revealed that cells treated with HSP70/HSP70-PCs healed faster than negative control cells. HSP70/HSP70-PCs-treated cells also exhibited better migration ability and higher invasion ability than control cells. HSP70/HSP70-PCs treatment did not alter the messenger RNA (mRNA) or protein levels of matrix metalloproteinase-9; the opposite was true for Ras homolog family member A (RhoA) mRNA and protein levels. RNA interference of RhoA attenuated the migration of HSP70/HSP70-PCs-treated cells. The present findings indicate that regulation of HCC cell migration by HSP70/HSP70-PCs occurs via regulation of RhoA expression. [ABSTRACT FROM AUTHOR]

Acceleration is of great importance in motion control for unmanned aerial vehicles (UAVs), especially during the takeoff and landing stages. However, the measured acceleration is inevitably polluted by severe noise. Therefore, a proper noise suppression procedure is required. This paper presents a novel method to reduce the noise in the measured vertical acceleration for a thrust-vectored tail-sitter vertical takeoff and landing (VTOL) UAV. In the new procedure, a Kalman filter is first applied to estimate the UAV mass by using the information in the vertical thrust and measured acceleration. The UAV mass is then used to compute an estimate of UAV vertical acceleration. The estimated acceleration is finally fused with the measured acceleration to obtain the minimum variance estimate of vertical acceleration. By doing this, the new approach incorporates the thrust information into the acceleration estimate. The method is applied to the data measured in a VTOL UAV takeoff experiment. Two other denoising approaches developed by former researchers are also tested for comparison. The results demonstrate that the new method is able to suppress the acceleration noise substantially. It also maintains the real-time performance in the final estimated acceleration, which is not seen in the former denoising approaches. The acceleration treated with the new method can be readily used in the motion control applications for UAVs to achieve improved accuracy. [ABSTRACT FROM AUTHOR]

The climatic conditions and soil characteristics of the Loess Plateau in China make it have a unique freeze–thaw process and the distribution of soil unfrozen water. In order to elucidate the spatio-temporal variation of soil unfrozen water during freeze–thaw period and its influencing factors under different ecological construction measures, The spatial and temporal variation of soil unfrozen moisture and its main influencing factors in five different ecological plots in the Loess Plateau during freeze–thaw period were studied by combining field monitoring and indoor calculation. The results showed that: (1) the spatiotemporal changes of unfrozen water content in soil were different under different vegetation restoration methods. The variation of unfrozen water content in fallow land was the largest at 20cm depth, while the variation in surface 10cm was the largest in the other four plots. (2) The average unfrozen water content of soil in the representative soil layer can be estimated more accurately, and the best representative soil layer in the five sample plots is concentrated in 20–30 cm. (3) During seasonal freeze–thaw period, the main controlling environmental factors of unfrozen water content are air temperature and surface temperature. The results can provide reference for soil protection and water resources management in northwest China, especially in the Loess Plateau.

It is becoming increasingly clear that environment factors during early life play a pivotal role in the development of allergic asthma. Among these, a traditional farm is one of the strongest protective environments, and the protective effects have been, at least in part, attributed to the high-level exposure to lipopolysaccharide (LPS) on farms. However, the underlying mechanisms remain elusive, especially in ovalbumin (OVA)-induced neonatal allergic asthma model. Here, we used the OVA-induced asthma model in two age groups, neonatal and adult, when mice were first sensitized with peritoneal OVA/alum as neonates and adults, respectively. LPS was injected in the peritoneal cavity before OVA/alum sensitization. The effects of LPS treatment on allergic airway inflammation in the lung and the immune milieu in the peritoneal cavity were determined and compared between these two age groups. We found that LPS treatment abrogated the development of Th2 allergic airway responses in the neonatal group. In the adult group, the ameliorated Th2 allergic responses were accompanied with Th17 responses and neutrophil infiltration upon LPS treatment. We further investigated the immune milieu in the peritoneal cavity to elucidate the underlying mechanisms of this age-dependent difference. Our data show that in neonatal mice, LPS treatment significantly reduced the number of inflammatory monocytes in the peritoneal cavity. In the adult group, LPS treatment shifted the function of these cells which associated with Th1 and Th17 polarization. Our results provide more evidence that immunity in early life is distinct from that in adults, especially in the peritoneal cavity, and emphasize the importance of timing for the intervention of allergic asthma. Our results suggest that LPS treatment during early life is protective for the development of Th2 allergic responses. On the other hand, it might lead to a more severe phenotype of asthma when dampening the Th2 responses in adult mice.

Compositional flow simulation, which is required for modeling enhanced-oil-recovery (EOR) operations, can be very expensive computationally, particularly when the geological model is highly resolved. It is therefore difficult to apply computational procedures that require large numbers of flow simulations, such as optimization, for EOR processes. In this paper, we develop an accurate and robust upscaling procedure for oil/gas compositional flow simulation. The method requires a global fine-scale compositional simulation, from which we compute the required upscaled parameters and functions associated with each coarse-scale interface or well block. These include coarse-scale transmissibilities, upscaled relative permeability functions, and so-called α-factors, which act to capture component flow rates in the oil and gas phases. Specialized near-well treatments for both injection and production wells are introduced. An iterative procedure for optimizing the α-factors is incorporated to further improve coarsemodel accuracy. The upscaling methodology is applied to two example cases, a 2D model with eight components and a 3D model with four components, with flow in both cases driven by wells arranged in a five-spot pattern. Numerical results demonstrate that the global compositional upscaling procedure consistently provides very accurate coarse results for both phase and component production rates, at both the field and well level. The robustness of the compositionally upscaled models is assessed by simulating cases with time-varying well bottomhole pressures that are significantly different from those used when the coarse model was constructed. The coarse models are shown to provide accurate predictions in these tests, indicating that the upscaled model is robust with respect to well settings. This suggests that one can use upscaled models generated from our procedure to mitigate computational demands in important applications such as well-control optimization. [ABSTRACT FROM AUTHOR]

PurposeLiquid biopsy is attracting attention as a method of real-time monitoring of patients with tumors. It can be used to understand the temporal and spatial heterogeneity of tumors and has good clinical application prospects. We explored a new type of circulating tumor cell (CTC) enrichment technology combined with next-generation sequencing (NGS) to analyze the correlation between genomic alterations in circulating tumor cells of hepatocellular carcinoma and the counts of mesenchymal CTCs and CTC-associated white blood cell (CTC-WBC) clusters.MethodsWe collected peripheral blood samples from 29 patients with hepatocellular carcinoma from January 2016 to December 2019. We then used the CanPatrol™ system to capture and analyze mesenchymal CTCs and CTC-WBC clusters for all the patients. A customized Illumina panel was used for DNA sequencing and the Mann–Whitney U test was used to test the correlation between mesenchymal CTCs, CTC-WBC cluster counts, and specific genomic changes.ResultsAt least one somatic hotspot mutation was detected in each of the 29 sequenced patients. A total of 42 somatic hot spot mutations were detected in tumor tissue DNA, and 39 mutations were detected in CTC-DNA, all of which included common changes in PTEN, MET, EGFR, RET, and FGFR3. The number of mesenchymal CTCs was positively correlated with the somatic genomic alterations in the PTEN and MET genes (PTEN, P = 0.021; MET, P = 0.008, Mann–Whitney U test) and negatively correlated with the somatic genomic alterations in the EGFR gene (P = 0.006, Mann–Whitney U test). The number of CTC-WBC clusters was positively correlated with the somatic genomic alterations in RET genes (P = 0.01, Mann–Whitney U test) and negatively correlated with the somatic genomic alterations in FGFR3 (P = 0.039, Mann–Whitney U test).ConclusionsWe report a novel method of a CTC enrichment platform combined with NGS technology to analyze genetic variation, which further demonstrates the potential clinical application of this method for spatiotemporal heterogeneity monitoring of hepatocellular carcinoma. We found that the number of peripheral blood mesenchymal CTCs and CTC-WBC clusters in patients with hepatocellular carcinoma was related to a specific genome profile.

Abdominal aortic aneurysms (AAAs) are typically asymptomatic, and there is a high mortality rate associated with aneurysm rupture. AAA pathogenesis involves extracellular matrix degradation, vascular smooth muscle cell phenotype switching, inflammation, and oxidative stress. There is increasing evidence of excessive adipocyte accumulation in ruptured AAA walls. These excessive numbers of adipocytes in the vascular wall have been closely linked with AAA progression. Perivascular adipose tissue (PVAT), a unique type of adipose tissue, can be involved in adipocyte accumulation in the AAA wall. PVAT produces various chemokines and adipocytokines around vessels to maintain vascular homeostasis through paracrine and autocrine mechanisms in normal physiological conditions. Nevertheless, PVAT loses its normal function and promotes the progression of vascular diseases in pathological conditions. There is evidence of significantly reduced AAA diameter in vessel walls of removed PVAT. There is a need to highlight the critical roles of cytokines, cells, and microRNA derived from PVAT in the regulation of AAA development. PVAT may constitute an important therapeutic target for the prevention and treatment of AAAs. In this review, we discuss the relationship between PVAT and AAA development; we also highlight the potential for PVAT-derived factors to serve as a therapeutic target in the treatment of AAAs.

Abstract Background Circulating tumour cells (CTCs), especially mesenchymal CTCs, are important determinants of metastasis, which leads to most recurrence and mortality in hepatocellular carcinoma (HCC). However, little is known about the underlying mechanisms of CTC colonisation in pre-metastatic niches. Methods Detection and classification of CTCs in patients were performed using the CanPatrol™ system. A lentiviral vector expressing Prrx1-targeting shRNA was constructed to generate a stable HCC cell line with low expression of Prrx1. The effect of Prrx1 knockdown on stemness, migration, and drug resistance of the cell line was assessed, including involvement of SDF-1/CXCR4 signalling. Promising clinical applications of an inhibitor of STAT3 tyrosine phosphorylation, C188–9, and specific blockade with CXCR4 antibody were explored. Results The number of mesenchymal CTCs in blood was closely associated with tumour recurrence or metastasis. Pre-metastatic niche-derived SDF-1 could downregulate Prrx1, which induced the stemness, drug resistance, and increased expression of CXCR4 in HCC cells through the STAT3 pathway in vitro. In vivo, mice bearing tumours of Prrx1 low-expressing cells had significantly shorter survival. In xenograft tumours and clinical samples, loss of Prrx1 was negatively correlated with increased expression of CXCR4 in lung metastatic sites compared with that in the primary foci. Conclusions These findings demonstrate that decreased expression of Prrx1 stimulates SDF-1/CXCR4 signalling and contributes to organ colonisation with blood CTCs in HCC. STAT3 inhibition and specific blockade of CXCR4 have clinical potential as therapeutics for eliminating organ metastasis in advanced HCC.

As the primary ecological construction measure on the Loess Plateau, check dams play an essential role in developing agricultural production, improving people’s production and life, and replenishing groundwater. Soil water infiltration is the most important way to replenish groundwater in the dam land. In order to investigate the water infiltration process of check dams, an empirical model suitable for the simulation of the dam land infiltration process was selected. The soil water infiltration process of the check dam was studied by a field test and a model simulation. The results showed that there were few macropores in the dam, and the water mainly moved downwards in the form of matrix flow. Moreover, the stable infiltration rate of the dam site was low, and its infiltration process could be divided into three stages: rapid infiltration, fluctuating infiltration, and stable infiltration. In addition, the infiltration rate of a non-silted dense layer was 2.4~5 times that of a silted dense layer. The Horton model had a good fitting effect on the water infiltration process of the check dam and thus was suitable for the simulation and prediction of the water infiltration process of the dam. The results can provide a theoretical basis for efficient soil water utilization and infiltration simulation of check dam land.

Based on the core, cast thin section, whole rock analysis, conventional physical properties and high pressure mercury intrusion test, the sedimentary diagenesis characteristics of rudist shoal in Cretaceous Mishrif Formation of H Oilfield, Iraq and its control on the reservoir were studied. The rudist shoal of the Mishrif Formation develops in the high-stand systems tract and is distributed in the high places of paleogeomorphology on the edge of platform with strong hydrodynamic force. According to the relative sea level changes, lithologic evolution and sedimentary structure characteristics of the rudist shoal, the single rudist shoal is divided into four lithologic sections: A, B, C and D, that is, low-angle cross-bedding pelletoids-rudist packstone, low-angle cross-bedding and parallel bedding arene-rudist grainstone, parallel bedding rudist gravel limestone, and horizontal bedding carbonaceous mudstone. The complete sedimentary sequence of a single rudist shoal is often disrupted. Several rudist shoals superimpose to form thick rudist shoal sediment. The single rudist shoal thickness and lithologic sections assemblage change regularly in vertical direction. The rudist shoal has the characteristics of “strong dissolution, weak cementation and strong compaction”, forming pore-type reservoir with intergranular pores, intergranular dissolved pores, mold pores, and dissolved pores. With mainly coarse pore throats larger than 5 μm, the reservoir is of medium-high porosity and high permeability. There is lithological reverse cycles inside single shoals and between single shoals, with content of mud crystals decreasing from the bottom to the top, dissolution increasing, cementation decreasing in strength, pore throats getting larger, and physical properties turning better. The rudist shoal of MB2-1 at the top of the high-stand systems tract has the largest thickness, moreover, subject to the strongest atmospheric freshwater leaching, this layer has the most significant dissolution and the largest pore throat, so it is the best reservoir of the Mishrif Formation. Key words: Iraq, Cretaceous, rudist shoal, sedimentary process, diagenetic evolution, reservoir characteristics

Previous studies have established that disturbed lymphocytes are involved in the pathogenesis of Vogt-Koyanagi-Harada (VKH) syndrome. Accordingly, glucocorticoids (GCs), with their well-recognized immune-suppressive function, have been widely used for treatment of VKH patients with acute relapses. However, the systemic response of diverse immune cells to GC therapy in VKH is poorly characterized. To address this issue, we analyzed immune cell subpopulations and their phenotype, as well as cytokine profiles in peripheral blood from VKH patients (n=25) and health controls (HCs, n=21) by flow cytometry and luminex technique, respectively. For 16 patients underwent GC therapy (methylprednisolone, MP), the aforementioned measurements as well as the transcriptome data from patients before and after one-week’s GC therapy were also compared to interrogate the systemic immune response to GC therapy. Lymphocyte composition in the blood was different in VKH patients and HCs. VKH patients had significantly higher numbers of T cells with more activated, polarized and differentiated phenotype, more unswitched memory B cells and monocytes, as compared to HCs. MP treatment resulted in decreased frequencies of T cells and NK cells, inhibited NK cell activation and T cell differentiation, and more profoundly, a marked shift in the distribution of monocyte subsets. Collectively, our findings suggest that advanced activation and differentiation, as well as dysregulated numbers of peripheral lymphocytes are the major immunological features of VKH, and GC therapy with MP not only inhibits T cell activation directly, but also affects monocyte subsets, which might combinatorically result in the inhibition of the pathogenic immune response.

The article focuses on a study related to simulation of in-situ conversion process (ICP). It mentions that robustness of the multiscale method has been verified over a number of physical-parameter ranges with a limited-scope sensitivity and the multiscale method consistently improves the simulation results and matches the fine-scale reference results closely.

In this study, a 3D multicomponent multiphase simulator with a new fracture characterization technique is developed to simulate the enhanced recovery of coalbed methane. In this new model, the diffusion source from the matrix is calculated using the traditional dual-continuum approach, while in the Darcy flow scale, the Discrete Fracture Model (DFM) is introduced to explicitly represent the flow interaction between cleats and large-scale fractures. For this purpose, a general formulation is proposed to model the multicomponent multiphase flow through the fractured coal media. The S&D model and a revised P&M model are incorporated to represent the geomechanical effects. Then a finite volume based discretization and solution strategies are constructed to solve the general ECBM equations. The prismatic meshing algorism is used to construct the grids for 3D reservoirs with complex fracture geometry. The simulator is validated with a benchmark case in which the results show close agreement with GEM. Finally, simulation of a synthetic heterogeneous 3D coal reservoir modified from a published literature is performed to evaluate the production performance and the effects of injected gas composition, well pattern and gas buoyancy. [ABSTRACT FROM AUTHOR]

Background: Ganoderma lucidum polysaccharides (GLPS) have been used as traditional Chinese medicine for their properties of cancer prevention and immunomodulation. However, it is unclear whether GLPS has therapeutic effect on anti-hepatocellular carcinoma (HCC) in vivo. In this study, the effect of GLPS and their impact on the balance of regulatory T cell (Treg) and effector T cell (Teff) was measured in a model of hepatoma-bearing mice. Methods: The effect of GLPS and their impact on the balance of regulatory T cell (Treg) and effector T cell (Teff) were measured in a model of hepatoma-bearing mice. Real-time PCR detected the levels of MicroRNAs (miRNAs) and mRNA. The effects of Tregs on Teff proliferation were determined via suppression assay. The mircroRNA-125b (miR-125b) inhibitor was used to down-regulate miR-125b expression. Results: GLPS significantly suppressed tumor growth in hepatoma-bearing mice associated with an increase of the ratio of Teffs to Tregs. Moreover, GLPS eliminate Treg suppression of Teff proliferation with an increase in IL-2 secretion. Addition of GLPS to treat T cells inhibited Notch1 and FoxP3 expression through increase of miR-125b expression. In hepatoma-bearing mice, miR-125b inhibitor obviously abolished the effect of GLPS on tumor growth. Conclusions: This finding provides the novel evidence for GLPS on inhibition of HCC through miR-125b inhibiting Tregs accumulation and function. [ABSTRACT FROM AUTHOR]

Aim: Circulating tumor cells (CTC) are a precursor to metastasis in several types of cancer and are occasionally found in the bloodstream in association with immune cells, such as white blood cells (WBCs). CTC-associated WBC (CTC-WBC) clusters can promote CTC appreciation and metastasis, suggesting that patients with CTC-WBC clusters found in the peripheral blood may have a worse prognosis. However, it is unclear whether CTC-WBC clusters are present in the peripheral blood of patients with hepatocellular carcinoma (HCC) and suggest a poor prognosis for HCC.Methods: We collected peripheral blood from 214 patients with HCC from January 2014 to December 2016. CanPatrol™ CTC analysis technology was used to isolate and count CTCs and CTC-WBC clusters in the patients' peripheral blood. Chi-squared analysis was used to calculate the correlation between the CTC-WBC clusters and clinicopathological characteristics. Kaplan–Meier survival analysis and Cox regression analysis were used to assess patient prognosis.Results: We used CanPatrol™ CTC analysis technology to count different types of CTCs and CTC-WBC clusters. The results showed that CTC-WBC clusters and tumor size (P = 0.001), tumor number (P = 0.005), portal vein tumor thrombus (P = 0.026), BCLC stage (P < 0.001), AFP level (P = 0.002), and total number of CTCs (P < 0.001) were statistically related. Cox regression analysis revealed that CTC-WBC clusters are an independent prognostic indicator of DFS (HR = 1.951, 95%CI:1.348–2.824, P < 0.001) and OS (HR = 3.026, 95%CI:1.906–4.802, P < 0.001) in HCC patients. Using Kaplan–Meier analysis, we found that positive CTC-WBC cluster patients had significantly shorter DFS and OS than patients with negative CTC-WBC (P < 0.001 and P < 0.001, respectively).Conclusions: CTC-WBC clusters in the peripheral blood are an independent predictor of DFS and OS, and their presence indicates poor prognosis in patients with HCC.

Abstract Daytime radiative cooling serving as a method to pump heat from objects on Earth to cold outer space is an attractive cooling option that does not require any energy input. Among radiative cooler structures, the multilayer‐ or photonic‐structured radiative cooler, composed of inorganic materials, remains one of the most complicated structures to fabricate. In this study, transparent sapphire‐substrate‐based radiative coolers comprising a simple structure and selective emitter‐like optical characteristics are proposed. Utilizing the intrinsic optical properties of the sapphire substrate and adopting additional IR emissive layers, such as those composed of silicon nitride thin film or aluminum oxide nanoparticles, high‐performance radiative coolers can be fabricated with a low mean absorptivity (3–4%) at 0.3–2.5 µm and a high mean emissivity of over 90% at 8–13 µm. Experiments show that the fabricated radiative coolers reach temperature drops of ≈10 °C in the daytime. From the theoretical calculations of radiative cooling performance, the sapphire‐substrate‐based radiative coolers demonstrate a net cooling power as high as 100 Wm−2.

This study addresses the task of supervised cross-domain image generation, which aims to translate an image from the source domain to the target domain, guided by a reference image from the latter. The key difference between the authors setting and the recently proposed domain guided photogeneration is that their image generation is bidirectional, i.e. images are generated in both domains. Thus, they call it bidirectional cross-domain image generation. This novel task poses new challenges as it requires the model learning to decouple personalised and shared semantics of the two domains. For this purpose, they propose a framework to learn a feature space, which breaks into three parts, namely, two personalised semantic subspaces, which encode patterns that are unique for each domain, and a shared semantic subspace that captures the common patterns. The three subspaces are automatically decoupled through end-to-end training. Extensive experiments on the modified shoe and handbag datasets show that their framework can generate high-quality images in both domains.

Electroencephalogram (EEG) as biomedical signal is widely applied in the medical field such as the detection of Alzheimer’s disease, Parkinson’s disease, etc. Moreover, by analyzing the EEG-based emotions, the mental status of individual can be revealed for further analysis on the psychological causes of some diseases such as cancer, which is considered as a vital factor on the induction of certain diseases. Therefore, once the emotional status can be correctly analyzed based on EEG signal, more healthcare-oriented applications can be furtherly carried out. Currently, in order to achieve efficiency and accuracy, diverse amounts of EEG-based emotions recognition methods generally extract features by analyzing the overall characteristics of signal, along with optimization strategy of channel selection to minimize the information redundancy. Those methods have been proved their effectiveness, however, there still remains a big challenge when applied with single channel information for emotion recognition task. Therefore, in order to recognize multidimensional emotions based on single channel information, an emotion quantification analysis (EQA) method is proposed to objectively analyze the semantically similarity between emotions in valence-arousal domains, and a multidimensional emotion recognition (EMER) model is proposed on recognizing multidimensional emotions according to the partial fluctuation pattern (PFP) features based on single channel information, and result shows that even though semantically similar emotions are proved to have similar change patterns in EEG signals, each single channel of 4 frequency bands can efficiently recognize 20 different emotions with an average accuracy above 93% separately.

An amendment to this paper has been published and can be accessed via the original article.

In recent years, the discovery of two gas fields in the fourth member of the Leikoupo Formation in the Western Sichuan Basin of SW China confirmed the exploration potential of microbial carbonates. The aim of the present study is to clarify the formation mechanism of the microbial reservoirs in the Leikoupo Formation. For this purpose, lithofacies, depositional environments, and diagenesis analyses were performed in samples collected from cores of 12 wells. The climate of study area was arid during Anisian time, and the water body was restricted. In such a climate, an evaporitic environment was developed, where ten types of lithofacies, dominated by microbial carbonates and gypsum rocks, were recognized. Thrombolites and stromatolites are the main high-quality reservoirs rock types in the fourth member of the Leikoupo Formation in the Western Sichuan Basin of SW China, which developed as microbial mounds, with reservoir space of microbial inter-clot pores, intra-clot pores, fenestral pores, inter-crystalline pores, and cracks. The microbial inter-clot pores are the main reservoir space, formed by trapping and binding of marls by benthic microbial communities. These pores were partially filled with evaporites because of the arid climate, which were subsequently dissolved (mainly gypsum) in the syn-depositional period, thus greatly improving the quality of reservoirs. Although some pores were occluded by multi-stage cements during the burial stage, major pores were well preserved own to the early dolomitization, rapid burial of the Leikoupo Formation, and early charging of hydrocarbon. The early dolomitization enhanced the anti-compaction ability of microbial carbonates during the burial stage. Rapid burial of the Leikoupo succession slowed down early cementation, and it also accelerated the maturation and expulsion process source rock to promote early charging of hydrocarbon in pores, which created a closed system, inhibiting strong burial cementation.

Mental disorder has been affecting numerous individuals; however, mental health care is in a passive state where only a minority of individuals actively seek professional help. Due to the rapid development of social networks, individuals accustomed to expressing their raw feelings on social media include patients who are suffering great pain from mental disorders. To distinguish individuals who merely feel sad and others who have mental disorders, the symptoms of mental disorder are taken into consideration. These symptoms constantly arise as a regular pattern like shifting of emotions or repeating of one representative emotion during a certain time. We proposed a Mental Disorder Identification Model (MDI-Model) to identify the four most commonly occurring mental disorders in the world: anxiety disorder, bipolar disorder, depressive disorder, and obsessive-compulsive disorder (OCD). The MDI-Model compares the sequential emotion pattern from users to identify mental disorders to detect those who are in a high risk. Tweets of diagnosed mental disorder users were analyzed to evaluate the accuracy of the MDI-Model, furthermore, the tweets of users from six different occupations were analyzed to verify the precision and predict the tendency of mental disorder among the different occupations. Results show that the MDI-Model can efficiently diagnose users with high precision in different mental statuses as severe, moderate, and mild stage, or tendency of mental disorder and mentally healthy status.

The article presents the Programmable light-driven swimming actuators via wavelength signal switching. Topics discussed include mechanical measurement system is established to quantitatively measure the driving force of the motion directly; and the accessible wavelength-selective strategy presented here can inspire further explorations of simple and practical light-driven materials and systems.

To clarify the effect of copper powder morphology on the microstructure and properties of copper matrix bulk composites reinforced with Ni-doped graphene, spherical and dendritic copper powders were selected to fabricate the Ni-doped graphene reinforced copper matrix bulk composites. The ni-doped graphene were synthesized by hydrothermal reduction method, followed by mixing with copper powders, and then consolidated by spark plasma sintering. It is found that the Ni-doped graphene are well bonded with the dendritic copper powder, whereas Ni-doped graphene are relatively independent on the spherical copper powder. The copper base bulk composite prepared by the dendritic copper powder has better properties than that prepared by spherical copper powder. At 0.5wt.% ni-doped graphene, the dendritic copper base bulk composite has a good combination of hardness, electrical conductivity and yield strength, which are 81.62 HV, 87.93% iaCS and 164 MPa, respectively. [ABSTRACT FROM AUTHOR]