351. Real-time cancellation of temperature induced resonance shifts in SOI wire waveguide ring resonator label-free biosensor arrays
- Author
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Martin Vachon, Jean Lapointe, S. Messaoudene, Rubin Ma, E. Post, Dan-Xia Xu, Jean-Marc Fedeli, Jens H. Schmid, Adam Densmore, André Delâge, Pavel Cheben, and Siegfried Janz
- Subjects
Silicon ,Time Factors ,Materials science ,chemistry.chemical_element ,Silicon on insulator ,Biosensing Techniques ,Resonator ,Optics ,Electricity ,Animals ,Staining and Labeling ,business.industry ,Dynamic range ,Spectrum Analysis ,Temperature ,Resonance ,Silicon Dioxide ,Cladding (fiber optics) ,Atomic and Molecular Physics, and Optics ,chemistry ,Immunoglobulin G ,Rabbits ,business ,Refractive index ,Biosensor - Abstract
A comprehensive investigation of real-time temperature-induced resonance shift cancellation for silicon wire based biosensor arrays is reported for the first time. A reference resonator, protected by either a SU8 or SiO(2) cladding layer, is used to track temperature changes. The temperature dependence of resonators in aqueous solutions, pertinent to biosensing applications, is measured under steady-state conditions and the operating parameters influencing these properties are discussed. Real-time measurements show that the reference resonator resonances reflect the temperature changes without noticeable time delay, enabling effective cancellation of temperature-induced shifts. Binding between complementary IgG protein pairs is monitored over 4 orders of magnitude dynamic range down to a concentration of 20 pM, demonstrating a resolvable mass of 40 attograms. Reactions are measured over time periods as long as 3 hours with high stability, showing a scatter corresponding to a fluid refractive index fluctuation of ± 4 × 10(-6) in the baseline data. Sensor arrays with a SU8 protective cladding are easy to fabricate, while oxide cladding is found to provide superior stability for measurements involving long time scales.
- Published
- 2010