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Your search keyword '"DNA Footprinting methods"' showing total 359 results
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359 results on '"DNA Footprinting methods"'

351. Isolation and assay of mitochondrial transcription termination factor from human cells.

Author

Micol V, Fernández-Silva P, and Attardi G

Subjects
Base Sequence, Chromatography, Affinity methods, DNA Footprinting methods, Deoxyribonuclease I, Electrophoresis, Polyacrylamide Gel methods, HeLa Cells, Humans, Immunoblotting methods, Indicators and Reagents, Molecular Sequence Data, Oligodeoxyribonucleotides, Restriction Mapping, DNA, Mitochondrial metabolism, DNA-Binding Proteins isolation & purification, DNA-Binding Proteins metabolism, Mitochondria metabolism, Transcription, Genetic
Published
1996
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352. In vitro analysis of transcription factor binding to nucleosomes and nucleosome disruption/displacement.

Author

Utley RT, Owen-Hughes TA, Juan LJ, Côté J, Adams CC, and Workman JL

Subjects
Binding, Competitive, Cell Fractionation methods, Cell Nucleus metabolism, Cell Nucleus ultrastructure, Centrifugation, Density Gradient methods, DNA Footprinting methods, DNA Probes, Deoxyribonuclease I, Electrophoresis, Polyacrylamide Gel methods, HeLa Cells, Histones isolation & purification, Histones metabolism, Humans, Indicators and Reagents, NF-kappa B metabolism, Nuclear Proteins metabolism, Nucleoplasmins, Nucleosomes ultrastructure, Protein Binding, Transcription Factors isolation & purification, Upstream Stimulatory Factors, DNA-Binding Proteins, Nucleosomes metabolism, Phosphoproteins, Transcription Factors metabolism
Published
1996
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353. In vitro assembly of enhancer complexes.

Author

Thanos D and Maniatis T

Subjects
Activating Transcription Factor 2, Chromatography, Affinity methods, Cloning, Molecular methods, Cyclic AMP Response Element-Binding Protein biosynthesis, DNA Footprinting methods, DNA Probes, Deoxyribonuclease I, Electrophoresis, Polyacrylamide Gel methods, Escherichia coli metabolism, HMGA1a Protein, High Mobility Group Proteins isolation & purification, Indicators and Reagents, Leucine Zippers, NF-kappa B biosynthesis, Nickel, Proto-Oncogene Proteins c-jun biosynthesis, Recombinant Proteins biosynthesis, Recombinant Proteins isolation & purification, Sequence Tagged Sites, Cyclic AMP Response Element-Binding Protein isolation & purification, Enhancer Elements, Genetic, High Mobility Group Proteins biosynthesis, NF-kappa B isolation & purification, Proto-Oncogene Proteins c-jun isolation & purification, Transcription Factors biosynthesis, Transcription Factors isolation & purification
Published
1996
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354. Genomic footprinting of mitochondrial DNA: I. In organello analysis of protein-mitochondrial DNA interactions in bovine mitochondria.

Author

Madsen CS, Ghivizzani SC, Ammini CV, Nelen MR, and Hauswirth WW

Subjects
Animals, Base Sequence, Cattle, Cloning, Molecular, DNA Probes, DNA, Mitochondrial isolation & purification, Genome, Indicators and Reagents, Methylation, Molecular Sequence Data, Piperidines, Regulatory Sequences, Nucleic Acid, Restriction Mapping, Sulfuric Acid Esters, DNA Footprinting methods, DNA, Mitochondrial chemistry, DNA, Mitochondrial metabolism, DNA-Binding Proteins metabolism, Mitochondria metabolism
Published
1996
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355. Purification of GAGA factor of Drosophila and its role in nucleosome disruption.

Author

Tsukiyama T and Wu C

Subjects
Adenosine Triphosphate metabolism, Animals, Chromatin isolation & purification, Chromatography, Affinity methods, Cloning, Molecular, DNA Footprinting methods, Deoxyribonuclease I, Drosophila melanogaster, Electrophoresis, Polyacrylamide Gel methods, Escherichia coli, HSP70 Heat-Shock Proteins biosynthesis, HSP70 Heat-Shock Proteins genetics, Indicators and Reagents, Micrococcal Nuclease, Promoter Regions, Genetic, Recombinant Proteins isolation & purification, Recombinant Proteins metabolism, Templates, Genetic, Chromatin metabolism, DNA-Binding Proteins, Drosophila Proteins, Homeodomain Proteins isolation & purification, Homeodomain Proteins metabolism, Nucleosomes physiology, Nucleosomes ultrastructure, Transcription Factors isolation & purification, Transcription Factors metabolism
Published
1996
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356. Reconstruction of transcriptionally active and silent chromatin.

Author

Ura K and Wolffe AP

Subjects
Animals, Cell Fractionation methods, Centrifugation, Density Gradient methods, Chickens, DNA metabolism, DNA Footprinting methods, Deoxyribonuclease I, Dialysis methods, Electrophoresis, Agar Gel methods, Erythrocytes metabolism, Erythrocytes ultrastructure, HeLa Cells, Humans, Indicators and Reagents, Kinetics, Nucleosomes metabolism, Nucleosomes ultrastructure, Templates, Genetic, Xenopus, Chromatin isolation & purification, Chromatin metabolism, DNA isolation & purification, Histones isolation & purification, Transcription, Genetic
Published
1996
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357. Quantitative kinetics footprinting of protein-DNA association reactions.

Author

Hsieh M and Brenowitz M

Subjects
DNA Footprinting instrumentation, DNA Footprinting methods, Deoxyribonuclease I, Indicators and Reagents, Kinetics, Models, Chemical, Phosphorus Radioisotopes, Protein Binding, Radioisotope Dilution Technique, DNA chemistry, DNA metabolism, DNA-Binding Proteins chemistry, DNA-Binding Proteins metabolism
Published
1996
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358. Structure of open promoter complexes with Escherichia coli RNA polymerase as revealed by the DNase I footprinting technique: compilation analysis.

Author

Ozoline ON and Tsyganov MA

Subjects
Alternative Splicing, Base Sequence, DNA Footprinting methods, DNA, Bacterial genetics, Databases, Factual, Escherichia coli genetics, Molecular Sequence Data, Sequence Homology, Nucleic Acid, Software, DNA, Bacterial metabolism, DNA-Directed RNA Polymerases metabolism, Deoxyribonuclease I, Escherichia coli enzymology, Promoter Regions, Genetic
Abstract

Footprinting data for 33 open promoter complexes with Escherichia coli RNA polymerase, as well as 17 ternary complexes with different regulators, have been compiled using a computer program FUTPR. The typical and individual properties of their structural organization are analyzed. Promoters are subgrouped according to the extent of the polymerase contact area. A set of alternative sequence elements that could be responsible for RNA polymerase attachment in different promoter groups is suggested on the basis of their sequence homology near the hyperreactive sites. The model of alternative pathways used for promoter activation is discussed.

Published
1995
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359. In vivo footprinting of protein-DNA interactions.

Author

Paul AL and Ferl RJ

Subjects
Alcohol Dehydrogenase genetics, Mutagens pharmacology, Sulfuric Acid Esters pharmacology, DNA Footprinting methods, DNA, Plant metabolism, DNA-Binding Proteins metabolism
Published
1995

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