351. Roles of Triolein and Lipolytic Protein in the Pathogenesis and Survival of Mycobacterium tuberculosis: a Novel Therapeutic Approach.
- Author
-
Monu and Meena LS
- Subjects
- Amino Acid Sequence, Butyrates metabolism, Carboxylic Ester Hydrolases chemistry, Carboxylic Ester Hydrolases metabolism, Carboxylic Ester Hydrolases therapeutic use, Fusarium enzymology, Humans, Hydrolysis, Lipolysis, Membrane Lipids chemistry, Membrane Lipids metabolism, Mycobacterium tuberculosis genetics, Mycobacterium tuberculosis pathogenicity, Sequence Homology, Amino Acid, Triolein metabolism, Tuberculosis enzymology, Tuberculosis therapy, Carboxylic Ester Hydrolases genetics, Fusarium genetics, Mycobacterium tuberculosis enzymology, Triolein chemistry, Tuberculosis genetics
- Abstract
Discovery of novel secreted enzymes and proteins in Mycobacterium tuberculosis (M. tuberculosis) are imperative to understanding the pathogenic system for pathogenesis requires attention. Till date, the groups of these secreted enzymes are not meaningfully characterized in terms of M. tuberculosis. In this way, cutinase, a small lipolytic protein, exists in both bacteria and fungi as well which have a potential catalytic activity. During our search, we have found a few genes of M. tuberculosis demonstrating a same significant lipase action as fungi Fusarium solani cutinase contain. Genome sequencing of M. tuberculosis uncover a lot of proteins, wherein (Rv1758, Rv1984c, Rv2301, Rv3451, Rv3452, Rv3724A, Rv3724B, and Rv3802c) genes have been noted which are exhibiting a cutinase-like activity and closely homologous to that of F. solani cutinase and having the ability to hydrolyze model substrates including p-nitrophenyl butyrate (p-PNB), cutin, triacylglycerols (TAGs), and triolein (TO), yet their biological significance in pathogenesis stays subtle and uncharacterized. In a basic perspective, the measure of cutinase expressed by M. tuberculosis and part of these small lipolytic enzymes in the pathologic discipline require thorough characterization. So, through focusing on cutinase-encoding genes in M. tuberculosis and their active catalytic motif could help to build up a novel therapeutic approach.
- Published
- 2016
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