301. ABP1 Mediates Auxin Inhibition of Clathrin-Dependent Endocytosis in Arabidopsis
- Author
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Stéphanie Robert, Eva Zažímalová, Elke Barbez, Michael Sauer, Pankaj Dhonukshe, Jiri Friml, Milada Čovanová, Zhenbiao Yang, Steffen Vanneste, Fernando Aniento, Jing Zhang, Alan M. Jones, Tomasz Paciorek, Sebastian Y. Bednarek, Christian Luschnig, Pawel Radoslaw Baster, Jürgen Kleine-Vehn, Sibu Simon, and Ken-ichiro Hayashi
- Subjects
0106 biological sciences ,Endosome ,media_common.quotation_subject ,Arabidopsis ,Receptors, Cell Surface ,Endocytosis ,01 natural sciences ,Clathrin ,General Biochemistry, Genetics and Molecular Biology ,Article ,03 medical and health sciences ,Auxin ,heterocyclic compounds ,PIN proteins ,Internalization ,030304 developmental biology ,media_common ,Plant Proteins ,chemistry.chemical_classification ,Auxin binding ,0303 health sciences ,biology ,Indoleacetic Acids ,Biochemistry, Genetics and Molecular Biology(all) ,Arabidopsis Proteins ,Cell Membrane ,fungi ,Membrane Transport Proteins ,food and beverages ,Receptor-mediated endocytosis ,Cell biology ,chemistry ,biology.protein ,010606 plant biology & botany - Abstract
SummarySpatial distribution of the plant hormone auxin regulates multiple aspects of plant development. These self-regulating auxin gradients are established by the action of PIN auxin transporters, whose activity is regulated by their constitutive cycling between the plasma membrane and endosomes. Here, we show that auxin signaling by the auxin receptor AUXIN-BINDING PROTEIN 1 (ABP1) inhibits the clathrin-mediated internalization of PIN proteins. ABP1 acts as a positive factor in clathrin recruitment to the plasma membrane, thereby promoting endocytosis. Auxin binding to ABP1 interferes with this action and leads to the inhibition of clathrin-mediated endocytosis. Our study demonstrates that ABP1 mediates a nontranscriptional auxin signaling that regulates the evolutionarily conserved process of clathrin-mediated endocytosis and suggests that this signaling may be essential for the developmentally important feedback of auxin on its own transport.
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