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Summary Die von H.E. Wichmann 1954 bei Augsburg auf Hippophae rhamnoides L. (Fam. Elaeagnaceae) gefundene Lachnus wichmanni n. sp. wird beschrieben. Neben Bemerkungen zur taxonomischen Charakterisierung der Gattung Lachnus werden die bis jetzt von Elaeagnaceen beschriebenen Lachniden kurz erwahnt.

The radioprotective effects of Hippophae rhamnoides (common name, sea buckthorn) leaf extract, designated SBL-1, were investigated in Swiss Albino strain ‘A’ mice. Against 100% mortality in whole-body irradiated (60Co-gamma-rays, 10 Gy) controls, a single dose of the SBL-1 rendered >90% survivors when administered 30 min before irradiation and 90% to 80% survivors when administered 1 to 4 h before irradiation. SBL-1 activated proliferation of hemopoietic stem cells countered a radiation-induced decrease in total thiols and an increase in free radicals in plasma and liver; inhibited lipid peroxidation, and normalized the liver alkaline phosphatase activity. This study demonstrated high radioprotective potential of H. rhamnoides leaves.

The potential role of host plant species in the selection of symbiotic, nitrogen-fixing Frankia strains belonging to the Elaeagnus host infection group was assessed in bioassays with two Morella, three Elaeagnus, and one Shepherdia species as capture plants, inoculated with soil slurries made with soil collected from a mixed pine/grassland area in central Wisconsin, USA. Comparative sequence analysis of nifH gene fragments amplified from homogenates of at least 20 individual lobes of root nodules harvested from capture plants of each species confirmed the more promiscuous character of Morella cerifera and Morella pensylvanica that formed nodules with frankiae of the Alnus and the Elaeagnus host infection groups, while frankiae in nodules formed on Elaeagnus umbellata, Elaeagnus angustifolia, Elaeagnus commutata, and Shepherdia argentea generally belonged to the Elaeagnus host infection group. Diversity of frankiae of the Elaeagnus host infection groups was larger in nodules on both Morella species than in nodules formed on the other plant species. None of the plants, however, captured the entire diversity of nodule-forming frankiae. The distribution of clusters of Frankia populations and their abundance in nodules was unique for each of the plant species, with only one cluster being ubiquitous and most abundant while the remaining clusters were only present in nodules of one (six clusters) or two (two clusters) host plant species. These results demonstrate large effects of the host plant species in the selection of Frankia strains from soil for potential nodule formation and thus the significant effect of the choice of capture plant species in bioassays on diversity estimates in soil.

Extensive degradation of irrigated croplands, due to increasing soil salinity and depletion of soil nutrient stocks, is a major problem in Central Asia (CA), one of the largest irrigated areas in the world. To assess the potential for improving the productive capacity of degraded lands by afforestation, we examined N(2) fixation of Elaeagnus angustifolia L. in mixed plantations with non-fixing Populus euphratica Oliv. and Ulmus pumila L. Fixation of N(2) was quantified by the (15)N natural abundance technique based on both foliar and whole-plant sampling during five consecutive growing seasons. Despite elevated root-zone soil salinity (6-10 dS m(-1)) and deficiency in plant-available P (4-15 mg kg(-1)), N(2) fixation (%Ndfa) increased from an initial value of 20% to almost 100% over 5 years. Within each growing season, %Ndfa steadily increased and peaked in the fall. Annual N(2) fixation, determined using foliar delta(15)N, initially averaged 0.02 Mg ha(-1), peaked at 0.5 Mg ha(-1) during the next 2 years and thereafter stabilized at 0.3 Mg ha(-1). Estimates based on whole-plant delta(15)N were

Seabuckthorn (Hippophae rhamnoides L.) is an underutilized plant, except in China and some European countries where it is comparatively popular. Seabuckthorn is a small fruit tree in the family Elaeagnaceae that originated in Central Asia. This species offers many advantages in terms of nutrition, health and environmental sustainability due to its vitamin-rich fruit and its use of symbiotic nitrogen fixation. It also offers advantages in terms of food security and income generation because it is stress-tolerant and can be used in processed products. We compared the nutritional composition of the fruit produced by two subspecies of seabuckthorn, H. rhamnoides ssp. mongolica and ssp. rhamnoides. The mature fruit of both subspecies contained high levels of beta-carotene (provitamin A) and alpha-tocopherol (vitamin E); however, the amounts of beta-carotene and alpha-tocopherol were greater in the fruit of ssp. mongolica than in that of ssp. rhamnoides. Seabuckthorn fruit also contained high levels of L-ascorbic acid (vitamin C). The levels of sugars and acids known to be important in fruit flavor were also measured in both subspecies. We also analyzed symbiotic nitrogen fixation and stress tolerance in the plants, which may facilitate extensive sustainable cultivation. Seabuckthorn is useful for the greening of non-arable land and deserts. Seabuckthorn root nodules showed high levels of nitrogen-fixation activity, similar to that seen in Rhizobium-legume root nodules. Even under nitrogen-free conditions, seabuckthorn plants with root nodules grew normally. The morphology and 16S ribosomal DNA sequence of the root nodules indicated that the nodules were formed by Frankia, which belongs to the Elaeagnus group. Finally, seabuckthorn exhibited tolerance to drought and high temperature stress. Our results indicate that small actinorhizal fruit trees like seabuckthorn may be used to ensure food security and sustainable production. Thus, further characterization and the introduction of appropriate small actinorhizal fruit trees should be pursued.

Aqueous and 70% ethanol extracts of seabuckthorn (Hippophae rhamnoides L., Elaeagnaceae) dry leaves were examined in rats for their dose dependent effect on active avoidance learning, if any. Avoidance learning was studied during endurance performance in multiple stressful environments consisting of light, noise and electric shock (10 mV) by using Runimex, a circular runway animal model. Neither of the evaluated extracts showed activity in rats to enhance cognitive functions with reference to avoidance learning during exposure to stressful conditions of multiple stressors. But both extracts were found to possess physical performance enhancing activity during the studied stressful conditions.

Processing effects on the mineral content were investigated during juice production from sea buckthorn (Hippophaë rhamnoides L. ssp. rhamnoides, Elaeagnaceae) using berries from 2 different growing areas. The major and trace elements of sea buckthorn berries and juices were determined by atomic absorption spectroscopy (AAS)--(calcium, iron, magnesium, potassium, sodium) and inductively coupled plasma-mass spectrometry (ICP-MS)--(arsenic, boron, chromium, copper, manganese, molybdenum, nickel, selenium, zinc). Potassium is the most abundant major element in sea buckthorn berries and juices. The production process increased the potassium content in the juice by about 20%. Moreover, the processing of juice increased the value of manganese up to 32% compared to the content in berries. During industrial juice production, the technological steps caused a loss of about 53% to 77% of the chromium concentration, 50% of the copper content, 64% to 75% of the molybdenum amount, and up to 45% of the iron concentration in the final juice product. Consumption of sea buckthorn juice represents a beneficial source of chromium, copper, manganese, molybdenum, iron, and potassium for the achievement of the respective dietary requirements.

Fructus Lycii is a traditional Chinese medicinal herb. The objective of this paper was to apply two-dimensional (2D) near-infrared (NIR) correlation spectroscopy to the discrimination of Fructus Lycii of four different geographic regions. Generalized 2D-NIR correlation spectroscopy was able to enhance spectral resolution, simplify the spectrum with overlapped bands, and provide information about temperature-induced spectral intensity variations that was hard to obtain from one-dimensional NIR spectroscopy. The 2D synchronous and asynchronous spectra showed remarkable differences within the range of 4950-5700cm(-1) between samples of different geographic regions. Using NIR instead of IR made the 2D approach more convenient and fast, and it can be applied to more area like process control. This approach can also be applied analogously to the discrimination of other Chinese herbal medicine of different geographic regions.

Homoploid hybrid plant species are rare, and the mechanisms of their speciation are largely unknown, especially for homoploid hybrid tree species. Two contrasting hypotheses have been proposed to explain the origin of Hippophae goniocarpa: (1) it is a diploid hybrid originating from H. rhamnoides ssp. sinensis x H. neurocarpa ssp. neurocarpa, and (2) it originated via marginal differentiation from H. rhamnoides ssp. sinensis. Regardless of which of these hypotheses is true (if either), previous studies have suggested that H. rhamnoides ssp. sinensis is the only maternal donor for this hybrid species. In this study, we aim to elucidate the maternal composition of H. goniocarpa and to test the two hypotheses. For this purpose, we sequenced the maternal chloroplast DNA trnL-F region of 75 individuals representing H. goniocarpa, H. rhamnoides ssp. sinensis, and H. neurocarpa ssp. neurocarpa in two co-occurring sites of the taxa. Seven haplotypes were identified from three taxonomic units, and their phylogenetic relationships were further constructed by means of maximum parsimony, maximum likelihood, and network analyses. These seven haplotypes clustered into two distinct, highly divergent lineages. Two haplotypes from one lineage were found in H. rhamnoides ssp. sinensis, and five (representing the other lineage) in H. neurocarpa ssp. neurocarpa. Hippophae goniocarpa shared four common haplotypes from both lineages, but the haplotypes detected from the two populations differed to some extent, and in each case were identical to local haplotypes of the putative parental species. Thus, both H. rhamnoides ssp. sinensis and H. neurocarpa ssp. neurocarpa appear to have together contributed to the maternal establishment of H. goniocarpa. These results clearly demonstrate that the marginal origin hypothesis should be rejected, and support the hybrid origin hypothesis. Hippophae goniocarpa exhibits a sympatric distribution with its two parent species, without occupying new niches or displaying complete ecological isolation. However, this species has effectively developed reproductive isolation from its sympatric parent species. Our preliminary results suggest that H. goniocarpa may provide a useful model system for studying diploid hybrid speciation in trees. (c) 2008 The Linnean Society of London.

There is a lack of studies on the occurrence and diversity of Frankia in African soils, including those in northern African regions. The present study on Tunisian soils is an attempt to address this issue using AInus glutinosa, Elaeagnus angustifolia and Casuarina glauca in a plant capturing bioassay on 30 soil samples, followed by amplified 16S ribosomal DNA restriction pattern analysis (ARDRA). A total of seven ARDRA haplotypes of Frankia have been detected in root actinorhizas that have been affiliated to theoretical ARDRA haplotypes upon in silico digestion of selected 16S ribosomal RNA (rRNA) gene sequences retrieved from GeneBank and confirmed by their partial 16S rRNA gene sequencing. F/aeagnus-compatible Frankia isolates were widespread and form four ARDRA haplotypes affiliated to Frankia, colonizing Elaeagnaceae and Rhamnaceae in two different phylogenetic subgroups. Alnus-compatible strains occurring in northern subhumid area were closely related to AInus-Morella-compatible strains and clustered in two ARDRA haplotypes. Casuarina-compatible strains lack variability in several northern arboreta. The relatively wide diversity of Tunisian Frankia strains opens the perspective that African soil could be an interesting reservoir for the isolation of new actinorhizal strains that could be used as potential biofertilizers to counteract the progressive soil desertification which indeed is a crucial environmental problem in Northern Africa.

Diversity of Frankia microsymbionts of non-native Elaeagnus angustifolia L. plants spontaneously growing in a Tunisian desertic retreat area, the date-palm oasis of Tozeur, was investigated by polymerase chain reaction – restriction fragment length polymorphism (PCR–RFLP) and PCR-sequencing techniques targeting the nifD-K intergenic spacer. Three PCR–RFLP haplotypes (I, II, and III) were detected among collected nodules. Haplotype I was detected at all five sampling sites and dominated the other haplotypes present at these sites. This haplotype was also exhibited by strain BMG5.10, which was isolated by a plant-capturing assay in 1998 from soil collected in the same locality, qualifying it to be the most competitive haplotype in the edapho-climatic condition of the studied desertic date-palm oasis. nifD-K sequences of the three haplotypes formed a closely related phylogenetic subgroup. These results suggest that Frankia variability is constrained by severe edapho-climatic conditions of retreated desert in Tunisian area.

In the present study, we analyzed the antioxidant, antiplatelet aggregation, anti-inflammatory, and tyrosinase inhibitory activities of a variety of solvent extracts of Elaeagnus multiflora Thunb. (Family Elaeagnaceae). Among the solvent extracts of E. multiflora, the ethyl acetate extract (EE) exhibited the highest 1,1-diphenyl-2-picrylhydrazyl radical and xanthine oxidase inhibition activity, as well as the greatest tyrosinase inhibition activity. Only the chloroform extract (CE) inhibited platelet aggregation, and that was a weak effect with 19.29% inhibition at 250 microg/mL, as compared to controls. The CE was also the most potent inhibitor of nitric oxide production among the tested fractions, with almost 100% inhibition at 500 microg/mL. We also detected 2,4-bis(1,1-dimethylethyl)phenol in the CE and EE, via a gas chromatography-mass spectrometry assay. In conclusion, we found that E. multiflora Thunb. has antioxidant and antiplatelet aggregation effects to some extent, and its CE and EE possess potent inhibitory effects on nitric oxide production and tyrosinase activity.

Seabuckthorn (Hippophae rhamnoides L.) is a small fruit tree belonging to the family Elaeagnaceae. Because seabuckthorn fruit is rich in unsaturated fatty acids and vitamins, this plant has potential as a food and medicinal crop. Here, we focused on symbiotic nitrogen fixation that could aid in the cultivation of this species. Microscopic observations showed that seabuckthorn root nodules have a standard morphology characteristic of Frankia-actinorhizal root nodules. Under nitrogen-free conditions, seabuckthorn seedlings inoculated with a homogenate of root nodules grew normally, and the fresh weight of root nodules was positively correlated with plant growth. In the field, nitrogenase activity in root nodules was high from May to September, when air temperatures were high and photosynthesis was active. Inhibition of nitrogen fixation by nitrate has been well documented in the root nodules of legumes. Therefore, we investigated the effect of nitrate on nitrogenase activity in seabuckthorn root nodules. Nitrogenase activity in seabuckthorn root nodules was not inhibited by the addition of high concentrations (up to 30 mM) of nitrate over a short term (5 days), but was apparently inhibited by long-term (20–30 days) treatments with 5 and 10 mM of nitrate.

Elaeagnus compatible Frankia isolates from Tunisian soil have been previously clustered with Frankia, colonizing Elaeagnaceae and Rhamnaceae in two different phylogenetic subgroups, while strain BMG5.6 was described as a new lineage closely related to Frankia and Micromonospora genera. In this study we further assess the diversity of captured Frankia and the relationship with BMG5.6-like actinobacteria, by using nifH gene sequences. Using PCR-RFLP screening on DNA extracted from lobe nodules, additional microsymbionts sharing BMG5.6 features have been detected proving a widespread occurrence of these actinobacteria in Elaeagnus root nodules. Neighbour-Joining trees of Frankia nifH sequences were consistent with previously published 16S rRNA and GlnII phylogenetic trees. Although four main clades could be discerned, actinobacterial strain BMG5.6 was clustered with Frankia strains isolated from Elaeagnus. The present study underscored the emanation of new diazotrophic taxon isolated from actinorhizal nodules occupying intermediate taxonomic position between Frankia and Micromonospora. Moreover, its aberrant position in nifH phylogeny should open network investigations on the natural history of nitrogen-fixing gene among actinobacteria.

The physicochemical properties, fatty acid profiles, content of tocopherol and sterol of the oils extracted from the nuts of Elaeagnus mollis Diels grown in different regions of China were studied in this work. The results indicated that the Elaeagnus mollis Diels nut oils contained about 0.2% sterols and the tocopherol contents were in the range of 119.6-128.6mg/100g. The nut oils were all rich in unsaturated fatty acids, especially oleic acid and linoleic acid. Furthermore, the main triacylglycerols species of the nut oils were all dilinoleoyl-monoolein (LOL), dioleoyl-monolinoleoyl (OLO) and trilinoleate (LLL). This work might be useful for developing applications for Elaeagnus mollis Diels nut oil.

To study and identify the three species of dry medicinal plant leaves trom Elaeagnus genus (E. pungens, E. lanceolata and E. henryi) by Infrared Spectroscopy(IR).Fourier transform infrared spectroscopy and second derivative infrared spectroscopy were used to study and compare the characteristics of leaves of three Elaeagnus medicinal plants.The IR spectra and second derivative infrared spectra of the three Elaeagnus plants leaves were similar on the whole, the intensity or ratio of intensity of some absorption peaks still had certain distinctions, and the differences of the second derivative infrared spectra were more obvious. There were only slight differences between large and small leaf type of samples of Elaeagnus lanceolata; the differences of the plant leaves of one species collected in different harvest periods were far smaller than those of others belonging to the same genus.IR can be relatively reliably used for identification of the three Elaeagnus leaves.

Sequences of the internal transcribed spacer (ITS) was compared in three seabuckthrons (Hippophae rhamnoides subsp. sinensis, H. tibetana and H. neurocarpa) distributed in Qinghai Province, then the systematic positions of 15 seabuckthron samples were analyzed with Elaeagnaceae angustifolia being outgroup. The results showed that ITS sequences in three seabuckthron species varied in length from 600 to 605 bp. ITS-1, 5.8S and ITS-2 varied from 201 to 203 bp, 166 to 167 bp and 232 to 236 bp, respectively. The sequence divergence among three seabuckthorn species was also remarkably high; Cluster analysis based on ITS indicated that H. gonicocarpa subsp. litangensis and H. gonicocarpa subsp. goniocarpa were distinct and could be classified as H. gonicocarpa and H. litangensis. H. gyantsensis had the closest genetic relationship with H. salicifolia and the distant relationship with H. rhamnoides subsp. sinensis. The positions of nine subspecies of H. rhamnoides based on morphological classification were different from the results of ITS.

Dissatisfaction from sexual relationships can result in deprivation as well as problems, such as depression, anxiety, and destruction of family’s mental health. One hundred twenty-five women (18 to 40 years) who suffered from hypoactive sexual desire disorder were divided into Elaeagnus angustifolia flower (4.5 g g daily for 35 days), sildenafil citrate tablet (50 mg for 4 weeks), and control groups. The study data were collected using the Female Sexual Function Index and Spielberger’s questionnaire and measurement of thyroid-stimulating hormone and prolactin hormone. In the Elaeagnus angustifolia group, the mean score of state and trait anxiety decreased after the intervention. In the sildenafil citrate group also, the mean score of state anxiety decreased from 22.15 ± 4.98 to 20.1 ± 5.15 ( P = .001) and that of trait anxiety decreased from 23.07 ± 4.44 to 21.55 ± 4.82 ( P = .002) after the intervention. Consumption of sildenafil citrate tablet was effective in reduction of the mean score of anxiety resulting from sexual dysfunction.

Objective Elaeagnus angustifolia L., commonly known as oleaster or Russian olive, is used worldwide as a traditional natural remedy or nutritional agent for the management of a wide range of illnesses. The aim of the present study is to comprehensively review the ethnopharmacological uses, biological activities and phytochemistry of E. angustifolia. Key findings Modern scientific investigations suggest that the plant possesses a broad spectrum of pharmacological and therapeutic effects, including antimicrobial, insecticidal, antioxidant, anti-arthritic, wound healing, cardioprotective, hypolipidemic, antinociceptive, anti-inflammatory, antimutagenic, antitumour and gastroprotective activity. Various phytochemical constituents, including β-carboline alkaloids, polysaccharides, esters, flavone glycosides, phenols, phenolic acids, ketones, phenyl ethers, pyrimidines, steroids and terpenes, as well as nutritional components such as essential and non-essential amino acids and nutraceuticals like vitamins and fatty acids, have been identified in E. angustifolia. Summary Considering its wide variety of pharmacological activity as well as its huge diversity of active phytochemicals, E. angustifolia could be proposed as a good candidate for discovering new drugs. Further clinical and experimental studies are necessary to reveal the beneficial therapeutic, safety and pharmaceutical properties of E. angustifolia and its phytochemicals as a complementary and alternative medicine for the management of various disorders.

The bark and fruit extracts of Elaeagnus umbellata have been investigated for their antibacterial, anti-fungal, insecticidal and phytotoxic activities. The petroleum ether extracts of the plant showed significant activity against E. faecalis. The activity of dichloromethane extract was also determined significant against S. aureus. The chloroform extract indicated low activity against E. coli, K. pneumoniae, B. subtilis and S. flexenari. The ethyl acetate fraction demonstrated significant activity against K. pneumoniae while methanolic extract exhibited significant activity against E. coli. All extracts showed low phytotoxic activity. The dichloromethane extract exhibited moderate insecticidal activity while other extract indicated low activity.

A new aphid species – Capitophorus wojciechowskii sp. n. – from northern Iran, associated with Hippophae rhamnoides and Hippophae sp., belonging to the tribe Macrosiphini (Hemiptera: Aphididae) is described. The fundatrix, apterous viviparous female, oviparous female and apterous male are described and illustrated. Similarities and differences with other Capitophorus species living on Elaeagnaceae are discussed. A key to Elaeagnaceae feeding aphids from the genus Capitophorus is also provided.

Elaeagnaceae, which harbor nitrogen-fixing actinomycetes, is a plant family of the Rosales and sister to Rhamnaceae, Barbeyaceae and Dirachmaceae. The results of previous molecular studies have not strongly supported the families of Elaeagnaceae, Rhamnaceae, Barbeyaceae and Dirachmaceae. However, chloroplast genome studies provide valuable phylogenetic information; therefore, we determined the chloroplast genome of Elaeaganus macrophylla and compared it to that of Rosales such as IR junction and infA gene. The chloroplast genome of Elaeagnus macrophylla is 152,224 bp in length and the infA gene of E. macrophylla was psuedogenation. Phylogenetic analyses based on 79 genes in 30 species revealed that Elaeagnus was closely related to Morus. Comparison of the IR junction in six other rosids revealed that the trnH gene contained the LSC region, whereas E. macrophylla contained a trnH gene duplication in the IR region. Comparison of the LSC/IRb (JLB) and the IRa/LSC (JLA) regions of Elaeagnaceae (Elaeagnus and Shephedia) and Rhamnaceae (Rhamnus) showed that trnH gene duplication only occurred in the Elaeagnaceae. The complete chloroplast genome of Elaeagnus macrophylla provides unique characteristics in rosids. The infA gene has been lost or transferred to the nucleus in rosids, while E. macrophylla lost the infA gene. Evaluation of the chloroplast genome of Elaeagnus revealed trnH gene duplication for the first time in rosids. The availability of Elaeagnus cp genomes provides valuable information describing the relationship of Elaeagnaceae, Barbeyaceae and Dirachmaceae, IR junction that will be valuable to future systematics studies.

The common sea-buckthorn ( Linnaeus, also known as Elaeagnus rhamnoides (Linnaeus) A. Nelson, or Moench) is a species of flowering plants of the family Elaeagnaceae, native to the dry temperate and cold desert areas of China, Russia, Mongolia, India, Pakistan, Nepal, Europe and North America. Comprising of eight subspecies, sea buckthorn is by far the most widespread species of the genus (Wu in Flora of China. Science Press and Missouri Botanical Garden Press, Beijing, 2007). The most common subspecies are H. rhamnoides (found in coastal North Europe), H. rhamnoides L. subsp. sinensis Rousi, H. rhamnoides L. subsp. gyantsensis Rousi, H. rhamnoides L. subsp. tibetana (Schlechtendal) Servettaz (found in China), and H. rhamnoides L. subsp. turkestanica Rousi (found in India, Afghanistan, Kazakhstan, Kyrgyzstan, Mongolia, Pakistan, Tajikistan, Turkmenistan and Uzbekistan).

Elaeagnus umbellata, a member of the Elaeagnaceae family, is native to Pakistan, China, India, Korea, and Japan. It is found commonly at altitudes ranging from 1200 to 2100 m and thrives on eroded and degraded land due to its ability to fix nitrogen. The plant also grows under variable pH (4–8) and drought, and is used locally as fuel wood, fencing, fodder, basket making, and shelterbelts. The fruit of the plant is well known for its essential nutrients and medicinal compounds such as vitamins, minerals, essential fatty acids, carotenoids (lycopene), soluble solids, and sugars. Medicinally, it is widely believed to protect against myocardial infections, pulmonary infections, and various forms of cancers. Ten ecotypes from variable microclimatic conditions were investigated for their morphological, molecular and biochemical diversity improvement and commercialization purposes. Comparisons and disabilities indicated significant variability in terms of morphological (plant height, number of branches, thorn s...

Fossil pollen records indicate that Hippophae rhamnoides (Sea Buckthorn) was widespread on late- and early postglacial raw soils throughout much of central and northern Europe, but that Early Holocene reforestation restricted populations to northern coastal habitats, or along mountain streams in the Alps, Pyrenees, and Carpathians. We used sequence variation at the nuclear chalcone synthase intron ( Chsi ), in conjunction with chloroplast DNA–restriction fragment length polymorphism data, to investigate the intraspecific phylogeny, phylogeographic structure, and expansion demographic history of this dioecious and wind-pollinated shrub at its range-wide scale in Europe and Asia Minor. Four major Chsi phylogroups of unresolved relationships were identified with estimated divergences ∼ ∼ ∼ 172 000 years ago. Large-scale phylogeographic structures of nuclear and cytoplasmic markers were congruent in identifying (i) southeastern Europe as the most likely source of colonization into central Europe and Scandinavia, and (ii) the area just north of the Alps as a contact zone between populations from the Alps and the east/central EuropeanScandinavian lineage. Coalescence-based analyses (i.e. nested clade analysis and mismatch distributions) of Chsi variation were able to detect at least four major episodes of population growth, all within about the last 40 000 years. In particular, these analyses identified a nearly synchronized timing of population expansions in various parts of the species’ range in central-eastern Europe/Asia Minor, most likely correlating with the Younger Dryas Stadial (∼ 13 000–11 600 years ago). It remains to be established whether the phylogeographic history of H. rhamnoides , and particularly its rapid response to the rapid environmental changes of the Younger Dryas cold snap, is unique to the species, or whether it is shared with other cold-tolerant shrub (or grassland) species known from late-glacial raw soils in Europe.

Elaeagnus angustifolia Linn. has various ecological, medicinal and economical uses. An approach was established using RP-HPLC (reversed-phase high-performance liquid chromatography) to classify and analyse the intra-specific genetic relationships of seventeen populations of E. angustifolia, collected from the Xinjiang areas of China. Chromatograms of alcohol-soluble proteins produced by seventeen populations of E. angustifolia, were compared. Each chromatogram of alcohol-soluble proteins came from a single seed of one wild plant only. The results showed that when using a Waters Delta Pak. C18, 5 microm particle size reversed phase column (150 mm x 3.9 mm), a linear gradient of 25%-60% solvent B with flow rate of 1 ml/min and run time of 67 min, the chromatography yielded optimum separation of E. angustifolia alcohol-soluble proteins. Representative peaks in each population were chosen according to peak area and occurrence in every seed. The converted data on the elution peaks of each population were different and could be used to represent those populations. GSC (genetic similarity coefficients) of 41% to 62% showed a medium degree of genetic diversity among the populations in these eco-areas. Cluster analysis showed that the seventeen populations of E. angustifolia could be divided into six clusters at the GSC=0.535 level and indicated the general and unique biochemical markers of these clusters. We suggest that E. angustifolia distribution in these eco-areas could be classified into six variable species. RP-HPLC was shown to be a rapid, repeatable and reliable method for E. angustifolia classification and identification and for analysis of genetic diversity.

The refraction indices of sea-buckthorn (Hippophae rhamnoides L. – Elaeagnaceae) oil for two wavelengths are measured in this study by using laser and Abbe refractometer at 632.8 and 589.3 nm, respectively. The transmission spectra in the visible and near infrared regions are obtained by using a Carry 5E spectrophotometer. Cauchy's and Sellmeier's coefficients were calculated and the dispersion curve n(λ) is obtained. Using only refractometric data, the oscillating (E 0) and dispersion (E d) energies are calculated. Refractometric analysis of sea-buckthorn (Hippophae rhamnoides L. – Elaeagnaceae) oil mixed with refined and non-refined sunflower oil is made as well.

Seedling morphology of woody plants indigenous in Japan are examined, mainly on the basis of materials from The Kyoto University Forest in Ashiu, Central Japan. Full descriptions for two species of Elaeagnaceae（two Elaeagnus spp.）, one of Tiliaceae（Tilia japonica）and seven of Araliaceae（Aralia elata,three Eleutherococcus spp., Gamblea innovans, Hedera rhombea,andKalopanax septemlobus）are provided with ecological notes and drawings., 【非公開】梅林正芳先生より、図譜刊行予定のためKURAでの公開をしない旨連絡があった（2015.11.6）

1,3-Dicapryloyl-2-linoleoylglycerol (1), a novel triglyceride, was isolated from berries of Hippophae rhamnoides. The structure was elucidated on the basis of MS, 1D and 2D NMR experiments including HMQC and HMBC. The metal chelating, free radical scavenging, and lipid peroxidation inhibiting properties of the compound were also estimated with particular reference to radiation protection. In case of metal chelation and superoxide ion scavenging, 1 showed maximum inhibition at 50 microg/ml (11%) and 100 microg/ml (55%), respectively, whereas in lipid peroxidation, 1 showed maximum inhibition (57%) at 2 mg/ml as compared to quercetin as a control.

Reflectance indices are frequently used for the nondestructive assessment of leaf chemistry, especially pigment content, in environmental or developmental studies. Since reflectance spectra are influenced by trichome density, and trichome density displays a considerable phenotypic plasticity, we asked whether this structural parameter could be a source of variation in the values of the most commonly used indices. Trichome density was manipulated in detached leaves of three species having either peltate (Olea europaea and Elaeagnus angustifolius) or tubular (Populus alba) trichomes by successive removal of hairs. After each dehairing step, trichome density was determined by light or scanning electron microscopy and reflectance spectra were obtained with a diode-array spectrometer. Although species-specific differences were evident, most of the indices were considerably affected even at low trichome densities. In general, the less-affected indices were those using wavebands within the visible spectral region. The index that could be safely used even at very high hair densities in all species was the red edge index (lambda(RE)) for chlorophyll. The results indicate that changes in reflectance indices should be interpreted cautiously when concurrent changes in trichome density are suspected. In this case, the red edge for chlorophyll content may be the index of choice.

Arbuscular mycorrhizas (AM) are the most widespread mycorrhiza in nature and form two morphologies, Arum- and Paris-type. The determining factors defining the two different morphologies are not well understood. In this study, the distribution of Arum- and Paris-type AM was determined in a mixed pine forest. A total of 35 plant species belonging to 20 families and 32 genera were identified and examined for AM colonization and morphological types. AM morphological types in 14 families were confirmed as follows: Arum-type in Rosaceae, Oleaceae, Lauraceae, Vitaceae and Compositae, Paris-type in Aquifoliaceae, Ulmaceae, Araliaceae, Theaceae, Magnoliaceae, Rubiaceae and Dioscoraceae, and both and/or intermediate types in Caprifoliaceae and Gramineae. Plant families whose AM morphological status was previously unknown were clarified as follows: Polygonaceae and Commelinaceae showed Arum-type morphology; Celastraceae, Menispermaceae and Elaeagnaceae had typical Paris-type morphology. The proportion of Arum-type to Paris-type species decreased in the following order: annuals > perennials > deciduous species > evergreen species, and pioneer group > early successional group > late successional group. Evergreen plants had a higher tendency to form Paris-type AM than annuals, perennials and deciduous plants. The results indicate that environmental changes, such as shade during plant succession, control the distribution of plant growth forms in mixed pine forest and may also play a part in the distribution of Arum- and Paris-type morphology. The identity of the plant seems to strongly influence AM morphology, though control by the fungal genome cannot be ruled out.

The occurrence and diversity of Frankia nodulating Elaeagnus angustifolia in Tunisia were evaluated in 30 soils from different regions by a Frankia-capturing assay. Despite the absence of actinorhizal plants in 24 of the 30 soils, nodules were captured from all the samples. Eight pure strains were isolated from single colonies grown in agar medium. On the basis of 16S rRNA and GlnII sequences, seven strains were clustered with Frankia, colonizing Elaeagnaceae and Rhamnaceae in two different phylogenetic groups while one strain described a new lineage in the Frankia assemblage, indicating that Frankia strains genetically diverse from previously known Elaeagnus-infective strains are present in tunisian soils. Genomic fingerprinting determined by rep-PCR, and tDNA-PCR-SSCP, confirmed the wide genetic diversity of the strains.

The wild edible plants form an important constituent of traditional diets in the Himalaya. In the Sikkim Himalaya a total of 190 species have been screened as edible species out of which nearly 47 species come to the market. The present paper deals with nutritive values of 27 most commonly consumed wild edible plants in the Sikkim Himalaya. Of 27 plant species that were analyzed for their nutritive values, 22 were edible for their fruits and five for leaves/shoots. Among different plant parts, generally higher nutrient concentration was recorded for leaves, followed by new shoots and fruits. For different species the crude fiber content ranged between 2.15–39.90%, and the total soluble salts between 4.66–21.0%, and the vitamin C content from 6-286 mg/100 g. The fat content was determined high in the fruits of Castanopsis species, Machilus edulis, and Cinnamomum species, while the protein content was highest in Hippophae rhamnoides, Cuc-umis melo, and Eleagnus latifolia. The total carbohydrate content ranged from 32-88% in the fruits of various wild edibles, the reducing sugar from 1.25–12.42%, total sugar from 2.10–25.09%, the lignin content varied from 9.05–39.51%, the hemicellulose between 25.63-55.71% and cellulose content varied from 9.57–33.19% in different species. Among the various mac-ronutrients estimated in the plant samples of different wild edible species, nitrogen was present in highest quantity, followed by potassium, calcium, magnesium, phosphorus, and sodium. Mi-cronutrients, such as iron, zinc, magnesium, and copper contents were analyzed in different plant parts of various wild edible species. The iron content was higher in leaves and new shoots. The study shows that wild edible plants are good source of nutrient for rural population, and also well comparable with various commercial fruits. It is suggested that a few wild edible species need to be grown for commercial cultivation and adopted in the traditional agroforestry systems, which will lead to reduced pressure on them in natural forest stands as well as producing economic benefits for poor farmers.

Functional adaptations of σ 70 transcriptional factors led to the emergence of several paralogous lineages, each one being specialized for gene transcription under particular growth conditions. Screening of a Frankia strain EaI-12 gene library by σ 70 DNA probing allowed the detection and characterization of a novel actinomycetal primary (housekeeping) σ 70 factor. Phylogenetic analysis positioned this factor in the RpoD cluster of proteobacterial and low-G+C-content gram-positive factors, a cluster previously free of any actinobacterial sequences. σ 70 DNA probing of Frankia total DNA blots and PCR screening detected one or two rpoD -like DNA regions per species. rpoD matched the conserved region in all of the species tested. The other region was found to contain sigA , an alternative primary factor. sigA appeared to be strictly distributed among Frankia species infecting plants by the root hair infection process. Both genes were transcribed by Frankia strain ACN14a grown in liquid cultures. The molecular phylogeny of the σ 70 family determined with Frankia sequences showed that the alternative actinomycetal factors and the essential ones belonged to the same radiation. At least seven distinct paralogous lineages were observed among this radiation, and gene transfers were detected in the HrdB actinomycetal lineage.

New information on (1) Hippophae goniocarpa Y.S. Lian et al. ex Swenson et Bartish and (2) H. goniocarpa ssp. litangensis (Lian et X.L. Chen ex Swenson et Bartish) Lian et K. Sun from China is provided and illustrated. Based on morphological characters and distribution, H. litangensis Y.S. Lian et X.L. Chen ex Swenson et Bartish is re-described as a subspecies. H. goniocarpa (including ssp. litangensis) is a distinct species and should be placed in Section Gyantsensis of Hippophae, although it has been suggested that sspp. litangensis and goniocarpa are the result of hybridization between H. rhamnoides ssp. sinensis and H. neurocarpa ssp. neurocarpa, and H. rhamnoides ssp. yunnanensis and H. neurocarpa ssp. stellatopilosa, respectively. The type subspecies of H. goniocarpa is distributed in Songpan and Hongyuan Counties of Sichuan Province and Qilian County of Qinghai Province, at 3500–3750 m, whereas ssp. litangensis is found only in Jiawa, Litang County, Sichuan Province at c. 3700 m. A key including the four taxa of sect. Gyantsensis is provided. © 2003 The Linnean Society of London, Botanical Journal of the Linnean Society, 2003, 142, 425−430.

Ten strains of Frankia isolated from root nodules of plant species from five genera of the host family Rhamnaceae were assayed in cross inoculation assays. They were tested on host plants belonging to four actinorhizal families: Trevoa trinervis (Rhamnaceae), Elaeagnus angustifolia (Elaeagnaceae), Alnus glutinosa (Betulaceae) and Casuarina cunninghamiana (Casuarinaceae). All Frankia strains from the Rhamnaceae were able to infect and nodulate both T. trinervis and E. angustifolia. Strain ChI4 isolated from Colletia hystrix was also infective on Alnus glutinosa. All nodules showed a positive acetylene reduction indicating that the microsymbionts used as inoculants were effective in nitrogen fixation. The results suggest that Frankia strains from Rhamnaceae belong to the Elaeagnus-infective subdivision of the genus Frankia.

Through biological inoculation technology, the joint symbiosis of Tibetan seabuckthorn (Hippophae tibetana) in pure culture was identified and the effects of dual inoculation with Frankia and mycorrhizal fungi on the host plants in pot cultures were investigated. The results obtained from the comparative study showed that H. tibetana could form nodules and VA mycorrhiza both in pot and pure cultures. VA mycorrhizae and Frankia can stimulate the growth and the nitrogen fixation ability of host plants, respectively, yet the stimulation of the dual inoculation on the growth and nitrogen fixation ability of the host plants was more significant (p

The small genus Hippophae (Elaeagnaceae) was thoroughly revised by Rousi in 1971. Several systematic treatments have since then confused the concept. Recent reports from China have added new subspecies, taxa with possible hybrid origin, and invalidly published names. Phylogenetic work on morphology and molecular markers agrees to Rousi's view of terminal taxa and seven species are currently recognised. Hippophae rhamnoides is a highly variable species and circumscribes eight subspecies. Recent work also confirms hybrid origins of two Chinese taxa. The purpose of this paper is to present a taxonomic synopsis of the genus with all currently accepted taxa and known types, especially in a situation of growing economic interest in Hippophae. We also provide a key and a map of the generic distribution.

Sea buckthorn (Hippophae rhamnoides L.) (Elaeagnaceae) has become of interest as a crop because of the nutritive qualities of its fruit. In this study, structural features of the fruit of H. rhamnoides ssp. cv. Indian Summer were examined by light microscopy. Additional information was obtained for branches, racemes, pedicels, female flowers, and seeds. Each fruit contains an embryo encased in a seed coat that is surrounded by a thin seed sac or pericarp with a persistent style, all of which is enclosed in the hypanthium. The hypanthium, which forms the fleshy portion of the fruit, contains vascular bundles, storage tissues, an endodermis, and an epidermis. The epidermis contains many trichomes and is confluent with the exterior of the pedicel. The calyx of the fruit is slightly open, and trichomes are present in the calyx opening, in the seed cavity, and on the tail of the seed sac. Harvest by removing the pedicel from the fruit rips the epidermis, which exposes fruit flesh and results in loss of juice from the fruit. Information on fruit structure is relevant to methods of harvesting.Key words: Hippophae, Elaeagnaceae, rhamnoides, mongolica Rousi, seed, microscopy.

Ziziphus Mill. (Rhamnaceae) is comprised of about 170 species that are mainly distributed in tropical to subtropical regions, with few in the temperate zone. Several Ziziphus fruit tree species are important energy, nutrient, and medicinal resources for human populations, particularly for those living in rural regions. To date, limited genomic information is available for this genus. Here, we assembled the complete chloroplast genomes of four best known Ziziphus species, i.e., Ziziphus jujuba, Ziziphus acidojujuba, Ziziphus mauritiana, and Ziziphus spina-christi, based on the Illumina Paired-end sequencing method. The chloroplast genomes of the four Ziziphus species are all very similar to one another, and exhibit structural, gene content, and order characteristics that are similar to other flowering plants. The entire chloroplast genome encodes 113 predicted unique genes (85 protein-coding genes, 8 rRNA, and 37 tRNA), 17 of which are duplicated in the inverted repeat regions. Rich single sequence repeats loci (217) were detected in Z. jujuba and 106 SSR loci, composed of A/T, displayed polymorphism across the four species by comparative genomic analysis. We found only four genes under positive selection between Z. jujuba and Z. acidojujuba, and two genes for Z. mauritiana vs. Z. spina-christi, respectively, while half of the 78 protein-coding genes experienced positive selection between the two groups. Phylogenetic analyses revealed that Ziziphus (Rhamnaceae) was sister to Elaeagnaceae, and the four species of Ziziphus were clustered into two groups (Z. jujuba and Z. acidojujuba, Z. mauritiana and Z. spina-christi). Our results provide genomic resources for intrageneric classifications of Ziziphus, and valuable genetic markers for investigating the population genetics and biogeography of closely related Ziziphus species.

Summary • The effects of N (ammonium nitrate), P (phosphate) and their interactions on nodulation were studied in the intercellularly infected actinorhizal plant Hippophae rhamnoides. • A split-root design, with pots receiving different concentrations of N and P, was used to determine whether the effects of N and P are local or systemic, if they are specific to nodulation or general, and whether P could counteract N inhibition. H. rhamnoides plants were grown for 6–10 wk after inoculation with Frankia. • Inhibition of nodulation by N was systemic for both nodule number and nodule biomass in H. rhamnoides. • By contrast, high P had a systemic stimulation on nodule number and biomass and P prevented systemic, but not local, N inhibition. Stimulation by P was specific to nodulation and not simply mediated via plant growth. Whether N and P alter not only nodulation but also N2-fixation in the nodules requires further investigation.

Morphology and molecular identification technology were used to identify 3 original plants of Fructus Elaeagni which was commonly used in Uygur medicine. Leaves, flowers and fruits from different areas were selected randomly for morphology research. ITS2 sequence as DNA barcode was used to identify 17 samples of Fructus Elaeagni. The genetic distances were computed by kimura 2-parameter (K2P) model, and the Neighbor-Joining (NJ) and Maximum Likelihood phylogenetic trees were constructed using MEGA5.0. The results showed that Elaeagnus angustifolia, E. oxycarpa and E. angustifolia var. orientalis cannot be distinguished by morphological characteristics of leaves, flowers and fruits. The sequence length of ITS2 ranged from 220 to 223 bp, the average GC content was 61.9%. The haplotype numbers of E. angustifolia, E. oxycarpa and E. angustifolia var. orientals were 4, 3, 3, respectively. The results from the NJ tree and ML tree showed that the 3 original species of Fructus Elaeagni cannot be distinguished obviously. Therefore, 3 species maybe have the same origin, and can be used as the original plant of Uygur medicineal material Fructus Elaeagni. However, further evidence of chemical components and pharmacological effect were needed.

•The Qinghai-Tibet Plateau is a major center of plant diversity and endemism, but little is known about how this developed due to the region's very scarce paleobotanical record. The silverberry genus Elaeagnus (Elaeagnaceae) reaches its greatest diversity (54 species) and endemism (36 species) in this area. Fossil Elaeagnaceae could provide significant evidence for the phylogeny and biogeography of the family and contribute primary data regarding the evolution of the unique Qinghai-Tibet Plateau flora in its dramatic setting of tectonic and climatic change.•We describe four fossil leaves with diagnostic features of Elaeagnus from the late Miocene of eastern Tibet, modern altitude of 3910 m a.s.l.. We also review prior fossil records of Elaeagnaceae.•The well-preserved, densely packed, stellate scales on fossil leaf surfaces are diagnostic of Elaeagnaceae. We assign these fossil leaves to Elaeagnus tibetensis T. Su et Z.K. Zhou sp. nov., comprising the first confirmed fossil Elaeagnus leaves worldwide.•Elaeagnus was present in eastern Tibet by the late Miocene. Together with previous fossil records, the new species supports a Holarctic history of the family. The diversification of Elaeagnus in the Qinghai-Tibet Plateau and adjacent areas might have been driven by continuous uplift at least since the late Miocene, causing formation of complex topography and climate with high rainfall seasonality. The characteristic scales on leaf surfaces are likely to be an important functional adaptation to seasonal droughts during early spring.

Elaeagnus oldhamii Maxim. is a commonly used traditional herbal medicine. In Taiwan the leaves of E. oldhamii Maxim. are mainly used for treating lung disorders. Twenty five compounds were isolated from the leaves of E. oldhamii Maxim. in the present study. These included oleanolic acid (1), 3-O-(Z)-coumaroyl oleanolic acid (2), 3-O-(E)-coumaroyl oleanolic acid (3), 3-O-caffeoyl oleanolic acid (4), ursolic acid (5), 3-O-(Z)-coumaroyl ursolic acid (6), 3-O-(E)-coumaroyl ursolic acid (7), 3-O-caffeoyl ursolic acid (8), 3β, 13β-dihydroxyolean-11-en-28-oic acid (9), 3β, 13β-dihydroxyurs-11-en-28-oic acid (10), uvaol (11), betulin (12), lupeol (13), kaempferol (14), aromadendrin (15), epigallocatechin (16), cis-tiliroside (17), trans-tiliroside (18), isoamericanol B (19), trans-p-coumaric acid (20), protocatechuic acid (21), salicylic acid (22), trans-ferulic acid (23), syringic acid (24) and 3-O-methylgallic acid (25). Of the 25 isolated compounds, 21 compounds were identified for the first time in E. oldhamii Maxim. These included compounds 1, 4, 5 and 8–25. These 25 compounds were evaluated for their inhibitory activity against the growth of non-small cell lung cancer A549 cells by the MTT assay, and the corresponding structure-activity relationships were discussed. Among these 25 compounds, compound 6 displayed the best activity against the A549 cell line in vitro (CC50 = 8.56 ± 0.57 μg/mL, at 48 h of MTT asssay). Furthermore, compound 2, 4, 8 and 18 exhibited in vitro cytotoxicity against the A549 cell line with the CC50 values of less than 20 μg/mL at 48 h of MTT asssay. These five compounds 2, 4, 6, 8 and 18 exhibited better cytotoxic activity compared with cisplatin (positive control, CC50 value of 14.87 ± 1.94 μg/mL, at 48 h of MTT asssay). The result suggested that the five compounds might be responsible for its clinical anti-lung cancer effect.