200 results on '"Zhu, Shidong"'
Search Results
152. Improved identification of the electrocardio-signal for pulsatile ventricular assist devices
- Author
-
Li, Xinghui, primary, Luo, Lin, additional, Zhu, Shidong, additional, Yang, Bibo, additional, Ni, Kai, additional, Zhou, Qian, additional, and Wang, Xiaohao, additional
- Published
- 2017
- Full Text
- View/download PDF
153. Improving hemodynamics of cardiovascular system under a novel intraventricular assist device support via modeling and simulations
- Author
-
Zhu, Shidong, primary, Luo, Lin, additional, Yang, Bibo, additional, Li, Xinghui, additional, and Wang, Xiaohao, additional
- Published
- 2017
- Full Text
- View/download PDF
154. Influence of heat stress on leaf morphology and nitrogen–carbohydrate metabolisms in two wucai (Brassica campestris L.) genotypes
- Author
-
Yuan, Lingyun, primary, Tang, Ling, additional, Zhu, Shidong, additional, Hou, Jinfeng, additional, Chen, Guohu, additional, Liu, Fan, additional, Liu, Shan, additional, and Wang, Chenggang, additional
- Published
- 2017
- Full Text
- View/download PDF
155. Response of osmotic adjustment and ascorbate-glutathione cycle to heat stress in a heat-sensitive and a heat-tolerant genotype of wucai ( Brassica campestris L.)
- Author
-
Zou, Mingqian, primary, Yuan, Lingyun, additional, Zhu, Shidong, additional, Liu, Shan, additional, Ge, Jitao, additional, and Wang, Chenggang, additional
- Published
- 2016
- Full Text
- View/download PDF
156. Comparative Proteomic Analysis Reveals That Chlorophyll Metabolism Contributes to Leaf Color Changes in Wucai (Brassica campestris L.) Responding to Cold Acclimation.
- Author
-
Xie, Shilei, Nie, Libing, Zheng, Yushan, Wang, Jie, Zhao, Mengru, Zhu, Shidong, Hou, Jinfeng, Chen, Guohu, Wang, Chenggang, and Yuan, Lingyun
- Published
- 2019
- Full Text
- View/download PDF
157. The New Research of Subsurface System Performance Curves of Sucker-Rod-Pumping
- Author
-
Chunming Xiong, Junfeng Shi, Xin Zhang, Zhu Shidong, Jinxiu Tian, Yuxia Wang, Jianjun Zhang, Ruidong Zhao, Tao Zhen, and Shicai Huang
- Subjects
Sucker rod ,Performance curves ,Geotechnical engineering ,Geology - Abstract
Abstract Sucker rod pumping system is the mechanical oil production method first used, up to now, it is also the most widely applied artificial lift method, however, three performance curves (displacement ~pump differential pressure, displacement ~ horsepower, displacement ~ efficiency) of sucker rod pumping (SRP), which are equivalent to those of electric submersible pump(ESP) and progressive cavity pump(PCP), are not available, increasing the complexity when node analyzing and designing of SRP well. As is known to all, when optimization design of SRP well, trial method is generally used and the operating time is long. Considering the interference of tubing, rod, wellbore fluid and piston pump when working, the subsurface system could be studied as a whole, and the relations between displacement and pump differential pressure, horsepower and pump differential pressure are researched. The dimensionless performance curves of SRP can be obtained based on API RP 11L standard. Three performance curves of subsurface system, namely the relation curves of displacement ~pump differential pressure, displacement ~ horsepower, displacement ~ efficiency, are drawn for the first time. The characteristics of these performance curves are analyzed, showing these curves described in this article can effectively reflect the working characteristics of the subsurface system of SRP. The subsurface performance curves can be a useful tool for sensitivity analysis and optimization design of SRP system. These curves allow developing a general nodal analysis algorithm for any artificial lift pumping method and a more convenient optimization design algorithm for composite pumping system, such as Jet- Rod pumping system. Introduction Sucker rod pumping downhole system can be seen as a special kind of plunger pump. There is a elastic rod string of several hundred to several thousand meters between polished rod and plunger pump, which is outstanding feature of the system[1]. When working underground, sucker rod pump is affected by elastic deformation of sucker rod and tubing, filling level of pump. So its performance is different from ordinary plunger pump. The curve which is called pump's performance curve shows relationships among the main parameters. Essentially, pump's performance curve is the external representation of fluid's motion in pump system[2]. So any pump system should have its own performance curve, which can generally be obtained by actual measurements, such as electric submersible pump. But the performance curve cannot be obtained by measuring the ground equipment like ESP because of sucker rod pump's long rod and complex underground environments. In order to get the performance curve of sucker rod pumping system, this paper is focused on API RP 11L standard[3] of American Petroleum Institute and put forward the concept of dimensionless performance curve of sucker rod pump which can be deduced by the curves in API RP 11L standard. The dimensionless performance curve can reflect the relationships among displacement, horsepower of polished rod, downhole efficiency and pump differential pressure. Due to assumptions of API method, the performance curve needs to be modified when applied in node analysis and optimization design.
- Published
- 2013
158. Comparative Proteomic Analysis Reveals That Chlorophyll Metabolism Contributes to Leaf Color Changes in Wucai (Brassica campestrisL.) Responding to Cold Acclimation
- Author
-
Xie, Shilei, Nie, Libing, Zheng, Yushan, Wang, Jie, Zhao, Mengru, Zhu, Shidong, Hou, Jinfeng, Chen, Guohu, Wang, Chenggang, and Yuan, Lingyun
- Abstract
Chlorophyll is a vital photosynthetic pigment that plays a key role in plant development, participating in light energy capture and energy conversion. In this study, a novel wucai (Brassica campestrisL.) germplasm with green outer leaves and yellow inner leaves at the adult stage (W7–2) was used to examine chlorophyll metabolism response to cold acclimation. A green leaf wucai genotype without leaf color changes named W7–1 was selected as the control to evaluate the chlorophyll metabolism changes of W7–2. Compared to W7–1, the contents of chlorophyll a(Chl a) and chlorophyll b(Chl b) in W7–2 were significantly reduced at five developmental stages (13, 21, 29, 37, and 45 days after planting (DAP)). An iTRAQ-based quantitative proteomic analysis was carried out at 21 and 29 DAP according to the leaf color changes in both of genotypes. 1409 proteins were identified, while 218 of them displayed differential accumulations between W7–2 and W7–1 during the two developmental stages. The differentially expressed proteins (DEPs) mainly assigned to chlorophyll biosynthesis, photosynthesis, carbohydrate metabolism, ribosome metabolism and posttranslational modification. Among these DEPs, NADPH-protochlorophyllide oxidoreductase (PORB) and Mg-protoporphyrin IX chelatase 1 (CHLI1) were the key enzymes participating in chlorophyll (Chl) biosynthesis, which was down-regulated at 21 DAP and up-regulated at 29 DAP in W7–2 compared with W7–1, respectively. The expression analysis of genes of three subunits of Mg-chelatase (CHLI1, CHLD, and CHLH), Genomes Uncoupled 4 (GUN4), and Thioredoxin (TRX3) associated with chlorophyll metabolism also displayed significant down-regulation in W7–2. In particular, PORBshowed significant up-regulation in W7–2, significantly affecting chlorophyll biosynthesis. Additionally, differences in chlorophyll metabolism between W7–2 and W7–1 were in terms of altered photosynthesis, carbohydrate, and energy metabolism. We found that the transcription levels of most photosynthesis proteins showed significantly lower levels, and the genes expression level, associated with carbohydrate and energy metabolism, were lower in W7–2 than in W7–1. Therefore, the present study results help understand the physiological and molecular mechanisms underlying leaf coloring responding to cold acclimation.
- Published
- 2019
- Full Text
- View/download PDF
159. Understanding the noble metal modifying effect on In2O3nanowires: highly sensitive and selective gas sensors for potential early screening of multiple diseasesElectronic supplementary information (ESI) available. See DOI: 10.1039/c9nh00404a
- Author
-
LiuThese authors contributed equally to this work., Wei, Sun, Jiao, Xu, Lin, Zhu, Shidong, Zhou, Xiangyu, Yang, Shuo, Dong, Biao, Bai, Xue, Lu, Geyu, and Song, Hongwei
- Abstract
Detection of multiple volatile organic compounds in human exhaled breath using semiconductor oxide-based sensing devices has attracted increasing attention for the early diagnosis of diseases. However, detecting trace levels of exhaled biomarkers with high sensitivity and selectivity remains a challenge and the corresponding mechanism for the selectivity is still unclear. Herein, ultrafine In2O3nanowires (NWs) modified with Au, Ag, and Pt noble metal nanoparticles (NMNPs) are synthesized using the electrospinning method and are used to build semiconductor sensors. It is exciting to observe that the introduction of NMNPs can not only enhance the sensing performance, but also effectively adjust the selectivity. The Au-, Ag- and Pt-modified In2O3NW sensors exhibit excellent selectivity to hydrogen sulfide, formaldehyde, and acetone biomarkers, respectively. To completely understand the mechanism, theoretical simulation is performed based on density functional theory, which demonstrated that the specific absorption energies between the modified In2O3NWs and the target gases along with the “spillover effect” resulted in excellent specific selectivity. Importantly, the NMNP modified In2O3NW sensors are successfully used to build a sensor array for effective simultaneous detection of simulated exhaled breath. The present work provides a simple, low-cost, effective tool for early screening of halitosis, breast cancer, and diabetes at the same time and an in-depth understanding of the enhanced sensing performance by the introduction of NMNPs.
- Published
- 2019
- Full Text
- View/download PDF
160. Regulation of 2,4-epibrassinolide on mineral nutrient uptake and ion distribution in Ca(NO3)2 stressed cucumber plants
- Author
-
Yuan, Lingyun, primary, Zhu, Shidong, additional, Shu, Sheng, additional, Sun, Jin, additional, and Guo, Shirong, additional
- Published
- 2015
- Full Text
- View/download PDF
161. Genome-Wide Identification of the Dicer-Like, Argonaute, and RNA-Dependent RNA Polymerase Gene Families in Cucumber (Cucumis sativus L.)
- Author
-
Gan, Defang, primary, Liang, Dandi, additional, Wu, Jiao, additional, Zhan, Mengdan, additional, Yang, Feng, additional, Xu, Wenjuan, additional, Zhu, Shidong, additional, and Shi, Jiayin, additional
- Published
- 2015
- Full Text
- View/download PDF
162. Relational-based transfer learning for automatic optical inspection based on domain discrepancy.
- Author
-
Valle Salgado, Erik I., Yan, Haoxin, Hong, Yue, Zhu, Peiyuan, Zhu, Shidong, Liao, Chengwei, Wen, Yanxiang, Li, Xiu, Qian, Xiang, Wang, Xiaohao, and Li, Xinghui
- Published
- 2022
- Full Text
- View/download PDF
163. A feature-based transfer-YOLOv5 model for rapid defect inspection in large mass magnetic tile manufacturing.
- Author
-
Li, Chen, Yan, Haoxin, Zhu, Shidong, Hong, Yue, Zhu, Peiyuan, Wen, Yanxiang, Tian, Haoyang, Liao, Chengwei, Li, Xiu, Wang, Xiaohao, Qian, Xiang, and Li, Xinghui
- Published
- 2022
- Full Text
- View/download PDF
164. A multi-source transfer learning-based weighted network for small sample defect inspection.
- Author
-
Hong, Yue, Li, Chen, Yan, Haoxin, Valle, Erik, Dou, Yi, Liao, Chengwei, Zhu, Peiyuan, Zhu, Shidong, Li, Xiu, Qian, Xiang, Wang, Xiaohao, and Li, Xinghui
- Published
- 2022
- Full Text
- View/download PDF
165. A novel calibration method for a uniaxial MEMS-based 3D reconstruction system.
- Author
-
Han, Min, Lei, Fengxiao, Tao, Yihao, Shi, Weijian, Lu, Shihao, Tian, Haoyang, Liao, Chengwei, Zhu, Peiyuan, Zhu, Shidong, Wang, Xiaohao, and Li, Xinghui
- Published
- 2022
- Full Text
- View/download PDF
166. Design and Implementation of Power Grid Dispatching Visualization Platform Based on SOA
- Author
-
Jiang, Liu, primary, Zhu, Shidong, additional, Yang, Sheng, additional, and Zhang, Xiaorui, additional
- Published
- 2013
- Full Text
- View/download PDF
167. AJAX-Based Reporting System of Scientific and Technological Achievements
- Author
-
Xiaorui, Zhang, primary, Chao, Sun, additional, Qingli, Wang, additional, Jiang, Liu, additional, and Zhu, Shidong, additional
- Published
- 2013
- Full Text
- View/download PDF
168. Research and Application of Private Cloud Storage Platform in High Schools Based on Seafile
- Author
-
Yang, Sheng, primary, Jiang, Liu, additional, Zhu, Shidong, additional, and Dai, Liang, additional
- Published
- 2013
- Full Text
- View/download PDF
169. The New Research of Subsurface System Performance Curves of Sucker-Rod-Pumping
- Author
-
Zhao, Ruidong, additional, Zhang, Jianjun, additional, Tao, Zhen, additional, Tian, Jinxiu, additional, Huang, Shicai, additional, Shi, Junfeng, additional, Xiong, Chunming, additional, Zhang, Xin, additional, Zhu, Shidong, additional, and Wang, Yuxia, additional
- Published
- 2013
- Full Text
- View/download PDF
170. Design and Implementation of Network Forensic System Based on Intrusion Detection Analysis
- Author
-
Jiang, Liu, primary, Tian, Guiyan, additional, and Zhu, Shidong, additional
- Published
- 2012
- Full Text
- View/download PDF
171. A feature-based transfer-YOLOv5 model for rapid defect inspection in large mass magnetic tile manufacturing
- Author
-
Dai, Qionghai, Shimura, Tsutomu, Zheng, Zhenrong, Li, Chen, Yan, Haoxin, Zhu, Shidong, Hong, Yue, Zhu, Peiyuan, Wen, Yanxiang, Tian, Haoyang, Liao, Chengwei, Li, Xiu, Wang, Xiaohao, Qian, Xiang, and Li, Xinghui
- Published
- 2023
- Full Text
- View/download PDF
172. Relational-based transfer learning for automatic optical inspection based on domain discrepancy
- Author
-
Dai, Qionghai, Shimura, Tsutomu, Zheng, Zhenrong, Valle Salgado, Erik I., Yan, Haoxin, Hong, Yue, Zhu, Peiyuan, Zhu, Shidong, Liao, Chengwei, Wen, Yanxiang, Li, Xiu, Qian, Xiang, Wang, Xiaohao, and Li, Xinghui
- Published
- 2023
- Full Text
- View/download PDF
173. A novel calibration method for a uniaxial MEMS-based 3D reconstruction system
- Author
-
Dai, Qionghai, Shimura, Tsutomu, Zheng, Zhenrong, Han, Min, Lei, Fengxiao, Tao, Yihao, Shi, Weijian, Lu, Shihao, Tian, Haoyang, Liao, Chengwei, Zhu, Peiyuan, Zhu, Shidong, Wang, Xiaohao, and Li, Xinghui
- Published
- 2023
- Full Text
- View/download PDF
174. Stability performance analysis for a new type assembled plastic greenhouse
- Author
-
Wu, Delin, primary, Tang, Qing, additional, and Zhu, Shidong, additional
- Published
- 2011
- Full Text
- View/download PDF
175. An improved phase measuring deflectometry method for defect detection of specular reflection surface.
- Author
-
Shi, Weijian, Lu, Shihao, Han, Min, Hu, Jiangyong, Liao, Chengwei, Zhu, Shidong, Qian, Xiang, Wang, Xiaohao, and Li, Xinghui
- Published
- 2021
- Full Text
- View/download PDF
176. Corrosion Mechanism of L360 Pipeline Steel Coated with S8 in CO 2 -Cl − System at Different pH Values.
- Author
-
Wang, Fan, Li, Jinling, Qu, Chengtun, Yu, Tao, Li, Yan, Zhu, Shidong, Yang, Bo, and Cheng, Frank
- Subjects
CARBON dioxide ,STEEL ,ELECTROLYTIC corrosion ,CURRENT distribution ,PITTING corrosion - Abstract
The corrosion behavior of L360 pipeline steel coated with or without elemental sulfur (S8) in CO
2 –Cl− medium at different pH was studied. An autoclave was used to simulate the working conditions for forming the corrosion scale, and an electrochemical workstation with a three-electrode cell was used to analyze the electrochemical characterization of the corrosion scale. A wire beam electrode was used to determine the potential and current distribution, and scanning electron microscopy and X-ray diffraction were used to characterize the morphology and composition of the corrosion scale. The results showed that the deposition of S8 on the surface of the electrodes caused serious localized corrosion, especially under acidic conditions. The morphology and localized corrosion intensity index further proved that the deposition of S8 significantly promoted corrosion, especially pitting corrosion. Finally, a novel corrosion mechanism of L360 pipeline steel coated with S8 in a CO2 -Cl− environment under acidic conditions was proposed, and we then modeled the theoretical mechanisms that explained the experimental results. [ABSTRACT FROM AUTHOR]- Published
- 2021
- Full Text
- View/download PDF
177. Birefringent filter with tilted optic axis for tuning dye lasers: theory and design
- Author
-
Zhu, Shidong, primary
- Published
- 1990
- Full Text
- View/download PDF
178. Corrosion Behavior of AlFeCrCoNiZr x High-Entropy Alloys in 0.5 M Sulfuric Acid Solution.
- Author
-
Yao, Yuhong, Jin, Yaohua, Gao, Wei, Liang, Xiaoyu, Chen, Jian, and Zhu, Shidong
- Subjects
HYDROCHLORIC acid ,SULFURIC acid ,ACID solutions ,CORROSION in alloys ,ALLOYS ,CORROSION resistance ,VACUUM arcs ,ELECTROLYTIC corrosion - Abstract
AlCoCrFeNiZrx (x = 0, 0.1, 0.2, 0.3, and 0.5) high-entropy alloys (HEAs) were prepared by a non-consumable vacuum arc melting technology, and the microstructure and corrosion behavior were investigated by XRD, SEM, immersion tests, and electrochemical measurements. The results indicate that galvanic corrosion of the AlCoCrFeNiZrx alloys occurred in 0.5 M H
2 SO4 solution, and only 0.1 mol of the added Zr could greatly improve the corrosion resistance of the alloys. The corrosion properties of the AlCoCrFeNiZrx HEAs had similar change tendencies with the increase in the Zr content in the immersion tests, potentiodynamic polarization measurements, and electrochemical impedance analysis, that is, the corrosion resistance of the AlCoCrFeNiZrx alloys in a 0.5 M H2 SO4 solution first increased and then decreased with the increase in the Zr content. The Zr0.1 alloys were found to have the best selective corrosion and general corrosion resistance with the smallest corrosion rate, whereas the Zr0.3 alloys presented the worst selective corrosion and general corrosion resistance with the highest corrosion rate from both the immersion tests and the potentiodynamic polarization measurements. [ABSTRACT FROM AUTHOR]- Published
- 2021
- Full Text
- View/download PDF
179. An improved phase measuring deflectometry method for defect detection of specular reflection surface
- Author
-
Yuan, Xiao-Cong, Carney, P. Scott, Shi, Kebin, Shi, Weijian, Lu, Shihao, Han, Min, Hu, Jiangyong, Liao, Chengwei, Zhu, Shidong, Qian, Xiang, Wang, Xiaohao, and Li, Xinghui
- Published
- 2021
- Full Text
- View/download PDF
180. Physiological and Transcriptomic Analyses Elucidate That Exogenous Calcium Can Relieve Injuries to Potato Plants (Solanum tuberosum L.) under Weak Light.
- Author
-
Hou, Jinfeng, Li, Jie, Yang, Yang, Wang, Zixin, Chang, Bowen, Yu, Xiaowei, Yuan, Lingyun, Wang, Chenggang, Chen, Guohu, Tang, Xiaoyan, and Zhu, Shidong
- Subjects
GAS exchange in plants ,PLANT injuries ,PHOTOSYNTHETIC rates ,CALCIUM ,SUPEROXIDE dismutase ,CARBON metabolism ,POTATOES - Abstract
Light is one of the most important abiotic factors for most plants, which affects almost all growth and development stages. In this study, physiological indicators suggest that the application of exogenous Ca
2+ improves photosynthesis and changes phytohormone levels. Under weak light, photosynthetic parameters of the net photosynthetic rate (PN ), stomatal conductance (Gs), and transpiration rate (Tr) decreased; the antioxidation systems peroxidase (POD), superoxide dismutase (SOD), and catalase (CAT) reduced; the degrees of malondialdehyde (MDA), H2 O2 , and superoxide anion (O2 − ) free radical damage increased; while exogenous Ca2+ treatment was significantly improved. RNA-seq analysis indicated that a total of 13,640 differently expressed genes (DEGs) were identified and 97 key DEGs related to hormone, photosynthesis, and calcium regulation were differently transcribed. Gene ontology (GO) terms and Kyoto encyclopedia of genes and genomes (KEGG) pathway analyses, plant hormone signal transduction, photosynthesis, carbon metabolism, and phenylpropanoid biosynthesis were significantly enriched. Additionally, quantitative real-time PCR (qRT-PCR) analysis confirmed some of the key gene functions in response to Ca2+ . Overall, these results provide novel insights into the complexity of Ca2+ to relieve injuries under weak light, and they are helpful for potato cultivation under weak light stress. [ABSTRACT FROM AUTHOR]- Published
- 2019
- Full Text
- View/download PDF
181. Developed method of excess fractions for calibrating the effective optical thickness of a Fabry–Perot etalon
- Author
-
Zhu, Shidong
- Abstract
A general method of calculating the integral-order number of interference is developed, completely without a trial method. The developed method of excess fractions is extended to the calibration of the effective optical thickness of a Fabry-Perot 6talon with the disperion of the refractive index and the phase shift on reflection. Several useful methods of calibration are provided and illustrated by examples. The condition under which the phase shift can be neglected is given for calculating the correct integral-order difference between wavelengths. A possible method of precise measurement of the dispersive phase shift is given. It is pointed out that exact knowledge of the integral order is not even necessary and that the phase shift can thus essentially be neglected for a precise measurement of the wavelength as long as the effective optical thickness is calibrated as the product of the fringe order of interference and the standard wavelength.
- Published
- 1989
182. Calculation of the integral-order number of interference of a Fabry–Perot interferometer-fringe system
- Author
-
Zhu Shidong
- Subjects
Physics ,business.industry ,Value (computer science) ,Interference (wave propagation) ,Atomic and Molecular Physics, and Optics ,Electronic, Optical and Magnetic Materials ,Wavelength ,Interferometry ,Reflection (mathematics) ,Optics ,Astronomical interferometer ,Computer Vision and Pattern Recognition ,business ,Fabry–Pérot interferometer ,Integer (computer science) - Abstract
A modified method of excess fractions used for finding the correct integral orders of interference is given. In this modified method, besides an approximate value of the interferometer spacing d′ ± Δd′, either two known precise wavelengths satisfying the requirement of |4(1/λ2 − 1/λ1)Δd′| ≤ 1 or three known precise wavelengths satisfying the requirement of 1 < |4(1/λ2 − 1/λ1)Δd′| ≤ n are sufficient for finding the correct integral orders. I take the integral part of either 2(d′ + Δd′)/λ1 or 2(d′ − Δd′)/λ1 as m11′, continue using m12′ + e2′ = (m11′ + e1)λ1/λ2, point out that the correct x in the expression m11 = m11′ + x when calculating the correct integral order is the integer nearest (e2 − e2′ − z)/(λ1/λ2 − 1), give the expression m12 = m12′ + x − z, introduce integer z into the above-mentioned expressions, and give the selection rules of z. In comparison with the traditional methods, this modified method will avoid both repeatedly probing calculation and using even more wavelengths in measurements and checking computation, and it has rigorous, simple, and convenient features.
- Published
- 1987
183. STRENGTH PROPERTIES ANALYSIS OF J55 TUBING PREMIUM CONNECTION
- Author
-
WANG Ke, WANG ShuangLai, ZHU ShiDong, YIN ZhiFu, and ZHANG YongQiang
- Subjects
J55 tubing ,Premium connection ,Finite element analysis ,Stress ,Sealing ring ,Mechanical engineering and machinery ,TJ1-1570 ,Materials of engineering and construction. Mechanics of materials ,TA401-492 - Abstract
Objective Analysis of torque screwing performance and four kinds of ultimate failure stress distribution of the J55 high strength no shoulder tubing premium connection,the inner wall of the tubing connection is smooth and alignment in favor of the eccentric wear of pumping rod. Methods Establish theoretical model for the specifications of 73. 02 × 5. 51 mm J55 high strength special thread joint pipe,stress analysis、four kinds of limit load failure( stretched to failure、compressed to failure、internal pressure to failure、external pressure to failure) stress distribution and the theoretical limit failure load analysis,stress distribution and rationality analysis of threaded joints under various conditions of the whole stress,the stress analysis of sealing ring contact. Results The finite element analysis shows that the stress distribution of the oil pipe torque screwing was reasonable,sealing rings were kept in high contact stress,and joints on the top end of the face as a assisting seal has been maintained at high pressure,the main seal and end assisting seal of Teflon seal ring were work together,the joints shows good sealing ability. The failure of four kinds of limit load( respectively as 408. 9 k N、584. 1 k N、84. 0 MPa、87. 0 MPa) are better than the strength requirements of the round thread in the API standard. The stress distribution of the threaded joint is reasonable. Conclusion The middle diameter of the threaded joint during the torque screwing can be reduced appropriately,in order to improve the performance of anti adhesion; in four kinds of limit load failure mode,the performance of high strength and special screw thread joint is good,accord with strength requirement of the specification of round thread in the API standard; the main seal and auxiliary seal effect of sealing ring is good.
- Published
- 2016
- Full Text
- View/download PDF
184. Complete mitochondrial genome assembly of Zizania latifolia and comparative genome analysis.
- Author
-
Luo X, Gu C, Gao S, Li M, Zhang H, and Zhu S
- Abstract
Zizania latifolia (Griseb.) Turcz. ex Stapf has been cultivated as a popular aquatic vegetable in China due to its important nutritional, medicinal, ecological, and economic values. The complete mitochondrial genome (mitogenome) of Z. latifolia has not been previously studied and reported, which has hindered its molecular systematics and understanding of evolutionary processes. Here, we assembled the complete mitogenome of Z. latifolia and performed a comprehensive analysis including genome organization, repetitive sequences, RNA editing event, intercellular gene transfer, phylogenetic analysis, and comparative mitogenome analysis. The mitogenome of Z. latifolia was estimated to have a circular molecule of 392,219 bp and 58 genes consisting of three rRNA genes, 20 tRNA genes, and 35 protein-coding genes (PCGs). There were 46 and 20 simple sequence repeats (SSRs) with different motifs identified from the mitogenome and chloroplast genome of Z. latifolia , respectively. Furthermore, 49 homologous fragments were observed to transfer from the chloroplast genome to the mitogenome of Z. latifolia , accounting for 47,500 bp, presenting 12.1% of the whole mitogenome. In addition, there were 11 gene-containing homologous regions between the mitogenome and chloroplast genome of Z. latifolia . Also, approximately 85% of fragments from the mitogenome were duplicated in the Z. latifolia nuclear genome. Selection pressure analysis revealed that most of the mitochondrial genes were highly conserved except for ccmFc , ccmFn , matR , rps1 , and rps3 . A total of 93 RNA editing sites were found in the PCGs of the mitogenome. Z. latifolia and Oryza minuta are the most closely related, as shown by collinear analysis and the phylogenetic analysis. We found that repeat sequences and foreign sequences in the mitogenomes of Oryzoideae plants were associated with genome rearrangements. In general, the availability of the Z. latifolia mitogenome will contribute valuable information to our understanding of the molecular and genomic aspects of Zizania ., Competing Interests: The authors declare that the research was conducted in the absence of any commercial or financial relationships that could be construed as a potential conflict of interest., (Copyright © 2024 Luo, Gu, Gao, Li, Zhang and Zhu.)
- Published
- 2024
- Full Text
- View/download PDF
185. V-Shape Pneumatic Torsional Actuator: A Building Block for Soft Grasper and Manipulator.
- Author
-
Ye X, Zhu S, Qian X, Zhang M, and Wang X
- Subjects
- Equipment Design, Humans, Torque, Robotics methods
- Abstract
Robotic joints are fundamental components in artificial graspers and manipulators, and they are designed to achieve high dexterity to carry out various tasks. Traditional robotic hands are often driven by rigid joints, such as tendons and electric motors, resulting in bulky and complex designs. Soft robotics, composed of flexible and compliant materials, offers promising solutions to mimic the human hands and handle delicate objects safely. In this article, we propose a simple yet effective V-shape pneumatic torsional actuator (V-PTA) as the building blocks of soft grasper and manipulators. The proposed actuator is capable of producing large angular changes and twisting torque, responding fast to pneumatic changes, and scaling to appropriate dimensions easily. Because the design is generic and modular in nature, these lightweight actuators could also be assembled to form a variety of patterns of structures to suit the needs of individual tasks. We further fabricated robotic graspers and manipulators based on multiple V-PTA and demonstrated that our design could offer solutions to execute daily laboratory operations that interact with small and irregular components. Experimental data confirmed that the V-PTA building block structure could potentially pave the path forward for miniature robotic automation in laboratory or industrial settings.
- Published
- 2022
- Full Text
- View/download PDF
186. Heat stress response in Chinese cabbage ( Brassica rapa L.) revealed by transcriptome and physiological analysis.
- Author
-
Zhang L, Dai Y, Yue L, Chen G, Yuan L, Zhang S, Li F, Zhang H, Li G, Zhu S, Hou J, Tang X, Zhang S, and Wang C
- Subjects
- Transcriptome genetics, Heat-Shock Response genetics, Gene Expression Profiling, Brassica rapa genetics, Brassica genetics
- Abstract
High temperatures have a serious impact on the quality and yield of cold-loving Chinese cabbage, which has evolved to have a unique set of stress mechanisms. To explore the relationship between these mechanisms and the heat-tolerance of Chinese cabbage, the physiological indicators of the heat-tolerant '268' line and heat-sensitive '334' line were measured. Under heat stress, the proline (Pro), soluble sugar (SS), and superoxide dismutase (SOD) indexes of the '268' line increased significantly. When additionally using transcriptome analysis, we found that the identified 3,360 DEGs were abundantly enriched in many metabolic pathways including 'plant hormone signal transduction', 'carbon metabolism', and 'glycolysis/gluconeogenesis'. Dynamic gene expression patterns showed that HKL1 in Cluster 15 may be a key factor in the regulation of sugar homeostasis. The interaction network screened four ABA-related genes in Cluster 15, suggesting that high temperatures lead to changes in hormonal signaling, especially an increase in ABA signaling. Compared with the '334' line, the expressions of Prx50 , Prx52 , Prx54 , SOD1 , and SOD2 in the '268' line were significantly upregulated, and these genes were actively involved in the reactive oxygen species (ROS) scavenging process. In summary, our results revealed the relationship between plant heat tolerance, physiology, and biochemistry and may also provide ideas for the future development of high-quality and heat-tolerant Chinese cabbage germplasm resources., Competing Interests: The authors declare that they have no competing interests., (© 2022 Zhang et al.)
- Published
- 2022
- Full Text
- View/download PDF
187. TMT-based comparative proteomic analysis of the male-sterile mutant ms01 sheds light on sporopollenin production and pollen development in wucai (Brassica campestris L.).
- Author
-
Tang X, Liu M, Chen G, Yuan L, Hou J, Zhu S, Zhang B, Li G, Pang X, and Wang C
- Subjects
- Biopolymers, Carotenoids, Flowers genetics, Flowers metabolism, Gene Expression Regulation, Plant, Hydrogen Peroxide metabolism, Plant Infertility genetics, Plant Proteins genetics, Plant Proteins metabolism, Pollen genetics, Pollen metabolism, Proteomics, Brassica genetics, Infertility metabolism
- Abstract
A spontaneous male-sterile mutant ms01 was discovered from the excellent high-generation inbred line 'hx12-6-3' in wucai. Compared with wild-type 'hx12-6-3', ms01 displayed complete male sterility with degenerated stamens and no pollen. In this study, cytological observation revealed that the tapetum of the anthers of ms01 had degraded in advance, and microspore development had stagnated in the mononuclear stage, ultimately resulting in completely aborted pollen. Genetic analysis indicated that the sterility of ms01 was controlled by a single recessive nuclear gene. In the differential proteomic analysis of 'hx12-6-3' and ms01 flower buds using a tandem mass tags-based approach, a comparison of two stages (stage a and stage e) revealed 1272 differentially abundant proteins (DAPs). The abnormal variation of the anther cuticle, pollen coat, and sporopollenin production were effected by lipid metabolism and phenylpropanoid biosynthesis in the mutant ms01. Further analysis elucidated that pollen development was associated with amino acid metabolism, protein synthesis and degradation, carbohydrate metabolism, flavonoid biosynthesis and glutathione metabolism. These results provide novel insights into the molecular mechanism of GMS (genic male sterility) in wucai. SIGNIFICANCE: ms01, as the first indentified spontaneous male-sterile mutant in wucai, plays a significant role in the initial study of GMS (genic male sterility). In our study, the key DAPs related to anther and pollen development were obtained by TMT-based comparative proteomic analysis. We found that the abnormal accumulation of H
2 O2 might induce premature degradation of the tapetum, causing anther metabolism disorder and pollen abortion. This process involved multiple DAPs and formed a complex regulatory network that generated a series of physiological metabolic alterations, ultimately leading to male sterility. Our results provide a theoretical foundation for further research on the complex anther and pollen development process., (Copyright © 2022 Elsevier B.V. All rights reserved.)- Published
- 2022
- Full Text
- View/download PDF
188. Transcriptome analysis and differential gene expression profiling of wucai (Brassica campestris L.) in response to cold stress.
- Author
-
Wang C, Zhang M, Zhou J, Gao X, Zhu S, Yuan L, Hou X, Liu T, Chen G, Tang X, Shan G, and Hou J
- Subjects
- Cold Temperature, Gene Expression Profiling, Gene Expression Regulation, Plant, Stress, Physiological genetics, Transcriptome, Brassica genetics, Cold-Shock Response genetics
- Abstract
Background: Wucai suffers from low temperature during the growth period, resulting in a decline in yield and poor quality. But the molecular mechanisms of cold tolerance in wucai are still unclear., Results: According to the phenotypes and physiological indexes, we screened out the cold-tolerant genotype "W18" (named CT) and cold-sensitive genotype "Sw-1" (named CS) in six wucai genotypes. We performed transcriptomic analysis using seedling leaves after 24 h of cold treatment. A total of 3536 and 3887 differentially expressed genes (DEGs) were identified between the low temperature (LT) and control (NT) comparative transcriptome in CT and CS, respectively, with 1690 DEGs specific to CT. The gene ontology (GO) analysis showed that the response to cadmium ion (GO:0,046,686), response to jasmonic acid (GO:0,009,753), and response to wounding (GO:0,009,611) were enriched in CT (LT vs NT). The DEGs were enriched in starch and sucrose metabolism and glutathione metabolism in both groups, and α-linolenic acid metabolism was enriched only in CT (LT vs NT). DEGs in these processes, including glutathione S-transferases (GSTs), 13S lipoxygenase (LOX), and jasmonate ZIM-domain (JAZ), as well as transcription factors (TFs), such as the ethylene-responsive transcription factor 53 (ERF53), basic helix-loop-helix 92 (bHLH92), WRKY53, and WRKY54.We hypothesize that these genes play important roles in the response to cold stress in this species., Conclusions: Our data for wucai is consistent with previous studies that suggest starch and sucrose metabolism increased the content of osmotic substances, and the glutathione metabolism pathway enhance the active oxygen scavenging. These two pathways may participated in response to cold stress. In addition, the activation of α-linolenic acid metabolism may promote the synthesis of methyl jasmonate (MeJA), which might also play a role in the cold tolerance of wucai., (© 2022. The Author(s).)
- Published
- 2022
- Full Text
- View/download PDF
189. Novel Pneumococcal Protein-Polysaccharide Conjugate Vaccine Based on Biotin-Streptavidin.
- Author
-
Guo M, Guo X, Zhang C, Zhu S, Zhang Y, Gu T, Kong W, and Wu Y
- Subjects
- Antibodies, Bacterial immunology, Antigens, Bacterial immunology, Chromatography, High Pressure Liquid, Humans, Immunity, Cellular, Immunity, Humoral, Immunization, Immunogenicity, Vaccine, Spectrum Analysis, Vaccine Development, Biotin, Pneumococcal Infections prevention & control, Pneumococcal Vaccines immunology, Streptavidin, Streptococcus pneumoniae immunology, Vaccines, Conjugate immunology
- Abstract
Pneumococcal disease is a serious public health problem worldwide and an important cause of morbidity and mortality among children and adults in developing countries. Although vaccination is among the most effective approaches to prevent and control pneumococcal diseases, approved vaccines have limited protective effects. We developed a pneumococcal protein-polysaccharide conjugate vaccine that is mediated by the noncovalent interaction between biotin and streptavidin. Biotinylated type IV capsular polysaccharide was incubated with a fusion protein containing core streptavidin and Streptococcus pneumoniae virulence protein and relied on the noncovalent interaction between biotin and streptavidin to prepare the protein-polysaccharide conjugate vaccine. Analysis of vaccine efficacy revealed that mice immunized with the protein-polysaccharide conjugate vaccine produced antibodies with high potency against virulence proteins and polysaccharide antigens and were able to induce Th1 and Th17 responses. The antibodies identified using an opsonophagocytic assay were capable of activating the complement system and promoting pathogen elimination by phagocytes. Additionally, mice immunized with the protein-polysaccharide conjugate vaccine and then infected with a lethal dose of Streptococcus pneumoniae demonstrated induced protective immunity. The data indicated that the pneumococcal protein-polysaccharide (biotin-streptavidin) conjugate vaccine demonstrated broad-spectrum activity applicable to a wide range of people and ease of direct coupling between protein and polysaccharide. These findings provide further evidence for the application of biotin-streptavidin in S. pneumoniae vaccines.
- Published
- 2022
- Full Text
- View/download PDF
190. Comparative transcriptome analysis reveals that chlorophyll metabolism contributes to leaf color changes in wucai (Brassica campestris L.) in response to cold.
- Author
-
Yuan L, Zhang L, Wu Y, Zheng Y, Nie L, Zhang S, Lan T, Zhao Y, Zhu S, Hou J, Chen G, Tang X, and Wang C
- Subjects
- Chlorophyll genetics, Gene Expression Regulation, Plant, Transcriptome, Brassica genetics, Brassica metabolism, Chlorophyll metabolism, Cold-Shock Response physiology, Pigmentation genetics, Pigmentation physiology, Plant Leaves metabolism
- Abstract
Background: Chlorophyll (Chl) is a vital photosynthetic pigment involved in capturing light energy and energy conversion. In this study, the color conversion of inner-leaves from green to yellow in the new wucai (Brassica campestris L.) cultivar W7-2 was detected under low temperature. The W7-2 displayed a normal green leaf phenotype at the seedling stage, but the inner leaves gradually turned yellow when the temperature was decreased to 10 °C/2 °C (day/night), This study facilitates us to understand the physiological and molecular mechanisms underlying leaf color changes in response to low temperature., Results: A comparative leaf transcriptome analysis of W7-2 under low temperature treatment was performed on three stages (before, during and after leaf color change) with leaves that did not change color under normal temperature at the same period as a control. A total of 67,826 differentially expressed genes (DEGs) were identified. Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway and Gene Ontology (GO) analysis revealed that the DEGs were mainly enriched in porphyrin and Chl metabolism, carotenoids metabolism, photosynthesis, and circadian rhythm. In the porphyrin and chlorophyll metabolic pathways, the expression of several genes was reduced [i.e. magnesium chelatase subunit H (CHLH)] under low temperature. Almost all genes [i.e. phytoene synthase (PSY)] in the carotenoids (Car) biosynthesis pathway were downregulated under low temperature. The genes associated with photosynthesis [i.e. photosystem II oxygen-evolving enhancer protein 1 (PsbO)] were also downregulated under LT. Our study also showed that elongated hypocotyl5 (HY5), which participates in circadian rhythm, and the metabolism of Chl and Car, is responsible for the regulation of leaf color change and cold tolerance in W7-2., Conclusions: The color of inner-leaves was changed from green to yellow under low temperature in temperature-sensitive mutant W7-2. Physiological, biochemical and transcriptomic studies showed that HY5 transcription factor and the downstream genes such as CHLH and PSY, which regulate the accumulation of different pigments, are required for the modulation of leaf color change in wucai under low temperature., (© 2021. The Author(s).)
- Published
- 2021
- Full Text
- View/download PDF
191. Transcriptional profiling reveals changes in gene regulation and signaling transduction pathways during temperature stress in wucai (Brassica campestris L.).
- Author
-
Yuan L, Zheng Y, Nie L, Zhang L, Wu Y, Zhu S, Hou J, Shan GL, Liu TK, Chen G, Tang X, and Wang C
- Subjects
- Cold Temperature, Gene Expression Profiling, Gene Expression Regulation, Plant, Photosynthesis genetics, Plant Proteins genetics, Plant Proteins metabolism, Stress, Physiological genetics, Temperature, Transcriptome, Brassica genetics, Brassica metabolism
- Abstract
Background: Wucai (Brassica campestris L. ssp. chinensis var. rosularis Tsen) is a cold-tolerant plant that is vulnerable to high temperature. This study explored the response mechanism of wucai to low temperature. In this study, wucai seedlings were treated with different temperatures, including low temperature (LT), high temperature (HT), and a control., Results: According to transcriptomics analysis, the number of differentially expressed genes (DEGs) in HT and LT was 10,702 and 7267, respectively, compared with the control. The key genes associated with the physiological response of wucai to the treatments were analyzed. The Kyoto Encyclopedia of Genes and Genomes and Gene Ontology annotations indicated the importance of the photosynthesis and photosynthetic-antenna protein pathways. We found that a high-temperature environment greatly inhibited the expression of important genes in the photosynthetic pathway (BrLhc superfamily members, PsaD, PsaE, PsaD, PsaD, PsbO, PsbP, PsbQ, PsbR, PsbS, PsbW, PsbY, Psb27, and Psb28), whereas low temperature resulted in the expression of certain key genes (BrLhc superfamily members, Psa F, Psa H, Psb S, Psb H, Psb 28). In addition, the wucai seedlings exhibited better photosynthetic performance under low-temperature conditions than high-temperature conditions., Conclusions: Based on the above results, we speculate that upon exposure to low temperature, the plants developed higher cold tolerance by upregulating the expression of genes related to photosynthesis. Conversely, high-temperature stress inhibited the expression of pivotal genes and weakened the self-regulating ability of the plants., (© 2021. The Author(s).)
- Published
- 2021
- Full Text
- View/download PDF
192. Morphological characteristics and transcriptome analysis at different anther development stages of the male sterile mutant MS7-2 in Wucai (Brassica campestris L.).
- Author
-
Wang J, Yang Y, Zhang L, Wang S, Yuan L, Chen G, Tang X, Hou J, Zhu S, and Wang C
- Subjects
- Flowers genetics, Gene Expression Profiling, Gene Expression Regulation, Plant, Plant Infertility genetics, Plant Proteins genetics, Transcriptome, Brassica genetics
- Abstract
Background: The discovery of male sterile materials is of great significance for the development of plant fertility research. Wucai (Brassica campestris L. ssp. chinensis var. rosularis Tsen) is a variety of non-heading Chinese cabbage. There are few studies on the male sterility of wucai, and the mechanism of male sterility is not clear. In this study, the male sterile mutant MS7-2 and the wild-type fertile plant MF7-2 were studied., Results: Phenotypic characteristics and cytological analysis showed that MS7-2 abortion occurred at the tetrad period. The content of related sugars in the flower buds of MS7-2 was significantly lower than that of MF7-2, and a large amount of reactive oxygen species (ROS) was accumulated. Through transcriptome sequencing of MS7-2 and MF7-2 flower buds at three different developmental stages (a-c), 2865, 3847, and 4981 differentially expressed genes were identified in MS7-2 at the flower bud development stage, stage c, and stage e, respectively, compared with MF7-2. Many of these genes were enriched in carbohydrate metabolism, phenylpropanoid metabolism, and oxidative phosphorylation, and most of them were down-regulated in MS7-2. The down-regulation of genes involved in carbohydrate and secondary metabolite synthesis as well as the accumulation of ROS in MS7-2 led to pollen abortion in MS7-2., Conclusions: This study helps elucidate the mechanism of anther abortion in wucai, providing a basis for further research on the molecular regulatory mechanisms of male sterility and the screening and cloning of key genes in wucai., (© 2021. The Author(s).)
- Published
- 2021
- Full Text
- View/download PDF
193. Characterization and transcriptomic analysis of a novel yellow-green leaf wucai (Brassica campestris L.) germplasm.
- Author
-
Nie L, Zheng Y, Zhang L, Wu Y, Zhu S, Hou J, Chen G, Tang X, Wang C, and Yuan L
- Subjects
- Chlorophyll, Gene Expression Profiling, Gene Expression Regulation, Plant, Photosynthesis genetics, Plant Leaves genetics, Plant Leaves metabolism, Plant Proteins genetics, Transcriptome, Brassica genetics, Brassica metabolism
- Abstract
Background: Leaf color mutants are the ideal materials to explore the pathways of chlorophyll (Chl) metabolism, chloroplast development, and photosynthesis system. In this study, a spontaneous yellow-green leaf wucai (Brassica campestris L.) mutant "WY16-13" was identified, which exhibited yellow-green leaf color during its entire growth period. However, current understanding of the molecular mechanism underlying Chl metabolism and chloroplast development of "WY16-13" is limited., Results: Total Chl and carotenoid content in WY16-13 was reduced by 60.92 and 58.82%, respectively, as compared with its wild type parental line W16-13. Electron microscopic investigation revealed fewer chloroplasts per cell and looser stroma lamellae in WY16-13 than in W16-13. A comparative transcriptome profiling was performed using leaves from the yellow-green leaf type (WY16-13) and normal green-leaf type (W16-13). A total of 54.12 million (M) (WY16-13) and 56.17 M (W16-13) reads were generated. A total of 40,578 genes were identified from the mapped libraries. We identified 3882 differentially expressed genes (DEGs) in WY16-13 compared with W16-13 (i.e., 1603 upregulated genes and 2279 downregulated genes). According to the Gene Ontology (GO) term and Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway analyses, these DEGs are involved in porphyrin and Chl metabolism [i.e., chlorophyllase (CLH), heme oxygenase (HO), chlorophyll (ide) b reductase (NYC), and protochlorophyllide oxidoreductase (POR) genes], carbohydrate metabolism, photosynthesis, and carbon fixation in photosynthetic organisms. Moreover, deficiency in Chl biosynthetic intermediates in WY16-13 revealed that the formation of the yellow-green phenotype was related to the disorder of heme metabolism., Conclusions: Our results provide valuable insights into Chl deficiency in the yellow-green leaf mutant and a bioinformatics resource for further functional identification of key allelic genes responsible for differences in Chl content.
- Published
- 2021
- Full Text
- View/download PDF
194. Cobalt-doped ZnO nanoparticles derived from zeolite imidazole frameworks: Synthesis, characterization, and application for the detection of an exhaled diabetes biomarker.
- Author
-
Zhu S, Xu L, Yang S, Zhou X, Chen X, Dong B, Bai X, Lu G, and Song H
- Subjects
- Biomarkers analysis, Biosensing Techniques, Catalysis, Cobalt chemistry, Electric Conductivity, Exhalation, Gases analysis, Humans, Limit of Detection, Oxidation-Reduction, Zinc Oxide chemistry, Acetone analysis, Diabetes Mellitus diagnosis, Imidazoles chemistry, Metal Nanoparticles chemistry, Metal-Organic Frameworks chemistry
- Abstract
Metal-organic frameworks (MOFs) with porous structures, high surface areas, diverse compositions, and functional linkers are promising materials and good carriers for building high-performance devices. In this work, uniform cobalt-doped ZnO nanoparticles (Co-doped ZnO NPs) derived from a MOF mold were synthesized, demonstrating the first example of synthesizing doped semiconductor metal oxide nanostructures using such strategy. The synthesis method produced Co-doped ZnO NPs that had a controllable doping mode, adjustable surface status, good dispensability, ferromagnetism and catalytic activity. The Co-doped ZnO NPs were evaluated as a sensing material for diabetes biomarker detection; the obtained sensors showed a high response to trace acetone (18.2 at 5 ppm), fast response/recovery times, a low detection limit (170 ppb), and long-term stability for 4 months. The enhanced sensing performance can be attributed to the increased number of active sites, additional impurity energy levels, and the catalytic ability of elemental Co. Moreover, the optimized sensor could distinguish between simulated diabetic breath and healthy human breath samples. The MOF-derived Co-doped ZnO NPs are a good candidate for the low-cost and noninvasive diagnosis of diabetes, and the proposed synthesis strategy can be extended to other types of extrinsically doped oxide materials., Competing Interests: Declaration of Competing Interest The authors declared that there is no conflict of interest., (Copyright © 2020. Published by Elsevier Inc.)
- Published
- 2020
- Full Text
- View/download PDF
195. Response of photosynthetic capacity and antioxidative system of chloroplast in two wucai ( Brassica campestris L.) genotypes against chilling stress.
- Author
-
Wang J, Fang R, Yuan L, Yuan G, Zhao M, Zhu S, Hou J, Chen G, and Wang C
- Abstract
Chilling stress during the growing season could cause a series of changes in wucai ( Brassica campestris L.). WS-1 (chilling-tolerant genotype) and Ta2 (chilling-sensitive genotype) were sampled in present study to explore the chilling tolerance mechanisms. Our results indicated that photosynthetic parameters exhibited lower level in Ta2 than in WS-1 under chilling stress. The rapid chlorophyll fluorescence dynamics curve showed that chilling resulted in a greater inactivation of photosystem II reaction center in Ta2. Reactive oxygen species and malondialdehyde content of chloroplast in Ta2 were higher than WS-1. The ascorbate-glutathione cycle in chloroplast of WS-1 played a more crucial role than Ta2, which was confirmed by higher activities of antioxidant enzymes including Ascorbate peroxidase, Glutathione reductase, Monodehydroascorbate reductase and Dehydroascorbate reductase and higher content of AsA and GSH. In addition, the ultrastructure of chloroplasts in Ta2 was more severely damaged. After low temperature stress, the shape of starch granules in Ta2 changed from elliptical to round and the volume became larger than that of WS-1. The thylakoid structure of Ta2 also became dispersed from the original tight arrangement. Combined with our previous study under heat stress, WS-1 can tolerant both chilling stress and heat stress, which was partly due to a stable photosynthetic system and the higher active antioxidant system in plants, in comparison to Ta2., (© Prof. H.S. Srivastava Foundation for Science and Society 2020.)
- Published
- 2020
- Full Text
- View/download PDF
196. Transcriptome analysis reveals a positive effect of brassinosteroids on the photosynthetic capacity of wucai under low temperature.
- Author
-
Zhao M, Yuan L, Wang J, Xie S, Zheng Y, Nie L, Zhu S, Hou J, Chen G, and Wang C
- Subjects
- Brassica drug effects, Brassica physiology, Steroids, Heterocyclic pharmacology, Brassica genetics, Brassica metabolism, Brassinosteroids pharmacology, Cold-Shock Response genetics, Gene Expression Profiling, Photosynthesis drug effects
- Abstract
Background: Brassinosteroids (BRs) have a positive effect on many processes during plant growth and development, and in response to various abiotic stressors. Low-temperature (LT) stress constricts the geographic distribution, growth, and development of wucai (Brassica campestris L. ssp. chinensis var. rosularis Tsen). However, there is little information on the global gene expression of BRs under LT stress in wucai. In this study, the molecular roles of 24-epibrassinolide (EBR) after exogenously application, were explored by RNA sequencing under LT conditions., Results: According to the Gene Ontology (GO) term and Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway analyses, photosynthesis was significantly enriched after spraying EBR under LT. The transcripts encoding the photosystem II (PSII) oxygen-evolving enhancer protein, photosystem I (PSI) subunit, light-harvesting chlorophyll protein complexes I and II, and ferredoxin were up-regulated after the application of EBR. Transcripts encoding several key enzymes involved in chlorophyll biosynthesis were also up-regulated, accompanied by significant differences in the contents of 5-aminolevulinic acid (ALA), porphobilinogen (PBG), protoporphyrin IX (Proto IX), Mg-protoporphyrin IX (Mg-proto IX), protochlorophyllide (Pchl), and photosynthetic pigments. Notably, transcriptional and physiological analyses revealed that under LT stress, plant responses to EBR involved a major reorientation of photosynthesis, as well as porphyrin and chlorophyll metabolism., Conclusion: This study explored the role of EBR as an LT stress tolerance mechanism in wucai. At the transcription level, LT tolerance manifests as an enhancement of photosynthesis, and the amelioration of porphyrin and chlorophyll metabolism.
- Published
- 2019
- Full Text
- View/download PDF
197. Transgenic Wucai (Brassica campestris L.) produced via Agrobacterium-mediated anther transformation in planta.
- Author
-
Chen G, Zeng F, Wang J, Ye X, Zhu S, Yuan L, Hou J, and Wang C
- Subjects
- Brassica metabolism, Plants, Genetically Modified metabolism, Pollination genetics, Pollination physiology, Agrobacterium tumefaciens genetics, Brassica genetics, Brassica physiology, Plants, Genetically Modified genetics, Plants, Genetically Modified physiology, Transformation, Genetic genetics
- Abstract
Key Message: We developed a novel Agrobacterium-mediated anther transformation for Wucai in planta, and in this procedure, the male germ line was the predominant target. Wucai (Brassica campestris L.), a variant of non-heading Chinese cabbage, is widely cultured in China and only improved by classic breeding methods. Here, a novel and efficient in planta Agrobacterium-mediated anther transformation method is developed based on the optimization of several factors that affect anther transformation. After optimization, transformation with the manual pollination application led to increased transient GUS expression in anthers (reaching 91.59%) and the transformation efficacies in planta (0.59-1.56% for four commercial cultivars). The stable integration and inheritance of the transgenes were further examined by molecular and genetic analyses. Three T
2 transgenic lines presented a segregation ratio of 3:1, which was consistent with the Mendelian feature of a single dominant gene. In addition, the GUS histochemical assay and genetic crossing analysis revealed that the male germ line was the predominant target in this transformation. This optimized transformation system could provide a useful tool for both the improvement of cultivar qualities and investigation of functional genes in Wucai.- Published
- 2019
- Full Text
- View/download PDF
198. Comparative Transcriptome Analysis between Fertile and CMS Flower Buds in Wucai (Brassica campestris L.).
- Author
-
Chen G, Ye X, Zhang S, Zhu S, Yuan L, Hou J, and Wang C
- Subjects
- Basic Helix-Loop-Helix Transcription Factors genetics, Comparative Genomic Hybridization, Flowers genetics, Gene Expression Regulation, Plant, Gene Ontology, Plant Breeding, Plant Proteins genetics, RNA, Plant chemistry, RNA, Plant isolation & purification, RNA, Plant metabolism, Sequence Analysis, RNA, Brassica genetics, Gene Expression Profiling methods, Genes, Plant, Plant Infertility genetics
- Abstract
Background: Wucai (Brassica campestris L. ssp. chinensis var. rosularis Tsen) is a variant of nonheading Chinese cabbage (Brassica campestris L.), which is one of the major vegetables in China. Cytoplasmic male sterility (CMS) has been used for Wucai breeding in recent years. However, the underlying molecular mechanism of Wucai CMS remains unclear. In this study, the phenotypic and cytological features of Wucai CMS were observed by anatomical analysis, and a comparative transcriptome analysis was carried out to identify genes related to male sterility using Illumina RNA sequencing technology (RNA-Seq)., Results: Microscopic observation demonstrated that tapetum development was abnormal in the CMS line, which failed to produce fertile pollen. Bioinformatics analysis detected 4430 differentially expressed genes (DEGs) between the fertile and sterile flower buds. Gene Ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway enrichment analyses were performed to better understand the functions of these DEGs. Among the DEGs, 35 genes (53 DEGS) were implicated in anther and pollen development, and 11 genes were involved in pollen cell wall formation and modification; most of these showed downregulated expression in sterile buds. In addition, several genes related to tapetum development (A6, AMS, MS1, MYB39, and TSM1) and a few genes annotated to flowering (CO, AP3, VIN3, FLC, FT, and AGL) were detected and confirmed by qRT-PCR as being expressed at the meiosis, tetrad, and uninucleate microspore stages, thus implying possible roles in specifying or determining the fate and development of the tapetum, male gametophyte and stamen. Moreover, the top four largest transcription factor families (MYB, bHLH, NAC and WRKY) were analyzed, and most showed reduced expression in sterile buds. These differentially expressed transcription factors might result in abortion of pollen development in Wucai., Conclusion: The present comparative transcriptome analysis suggested that many key genes and transcription factors involved in anther development show reduced gene expression patterns in the CMS line, which might contribute to male sterility in Wucai. This study provides valuable information for a better understanding of CMS molecular mechanisms and functional genome studies in Wucai.
- Published
- 2018
- Full Text
- View/download PDF
199. [Ecophysiological effects of CO2 enrichment on bottle gour in plastic house].
- Author
-
Zhu S and Xu W
- Subjects
- Agriculture, Carbohydrate Metabolism, Crops, Agricultural, Photosynthesis, Carbon Dioxide metabolism, Cucurbitaceae physiology
- Abstract
In vegetable growing under protection, lack of carbon dioxide is one of the most important factors limiting photosynthesis. The application of 600-1200 mumol.mol-1 CO2 in the plastic house increased photosynthetic rates and ratio of C/N, promoted both vegetative and reproductive growth of bottle gourd plants. Plant height, stem diameter, leaf thickness and leaf area increased by 3.90-19.48%, 11.58-27.37%, 38.46-69.23% and 26.09-49.38%, respectively. Average single fruit weight and yield were 4.05-19.62% and 8.65-19.47% higher than that in normal air. Primary female flower position was lowered by 2.6-4.0. Net photosynthetic rate and the carboxylase activity of RuBP were greatest under CO2 1000 mumol.mol-1. The optimal concentration of CO2 applied to bottle gourd in spring was about 1000 mumol.mol-1.
- Published
- 2002
200. [Characteristics of functional and nutritious soilless culture substrate for vegetables].
- Author
-
Zhu S, Xu W, and Zhao G
- Subjects
- Cucumis sativus growth & development, Solanum lycopersicum growth & development, Nitrogen metabolism, Phosphorus metabolism, Potassium metabolism, Culture Media chemistry, Vegetables growth & development
- Abstract
A functional and nutritious substrate for soilless culture, which consists of peanut shell, sawdust, vermiculite, chicken manure, coal cinder, etc, was used to grow cucumbers, tomatoes and peppers in this experiment. The results showed that the substrate was rich in organic matter, N, available P and K, in which the nutrients were basically in balance for three vegetables. High content of microorganisms and high activity of soil enzymes were propitious to the transformation of organic components in the substrate. The yields of cucumber, tomato and pepper increased by 23.83%, 27.34% and 32.98%, respectively. The production value of peppers increased by 180.85%, and its net income increased by 109.69%. The qualities of three vegetables were coincident with 'harmless vegetable' standards.
- Published
- 2002
Catalog
Discovery Service for Jio Institute Digital Library
For full access to our library's resources, please sign in.