251. Analysis of apolipoprotein A5 gene polymorphisms in Hubei Han people
- Author
-
You-Li Zhou, Yan Ding, Gong-Li Yang, Zhixiao Wang, and Ming-An Zhu
- Subjects
Male ,China ,Genotype ,Single-nucleotide polymorphism ,Biology ,medicine.disease_cause ,Polymerase Chain Reaction ,Asian People ,Gene Frequency ,Polymorphism (computer science) ,Apolipoprotein a5 ,Ethnicity ,medicine ,Humans ,Allele ,Gene ,Apolipoproteins A ,Aged ,Genetics ,Mutation ,Polymorphism, Genetic ,Racial Groups ,General Medicine ,Middle Aged ,Molecular biology ,Apolipoprotein A-V ,Female ,Gene polymorphism ,Polymorphism, Restriction Fragment Length - Abstract
Polymerase chain reaction-restriction fragments length polymorphism (PCR-RFLP) was used to explore the distribution of apolipoprotein A5 gene -1131T>C and 56C>G polymorphisms in 257 healthy Hubei Han people. The following results were calculated: the frequency of -1131TT genotype was 50.9%, far more than that of -1131TC and -1131CC genotypes (32.9% and 16.2%, respectively). The number of T allele carriers was higher than that of C carriers, and their respective frequencies were 0.675 and 0.325. There were 56GG and 56GC genotypes, but only 2 individuals in all subjects carried the G allele, the frequency of which was low than 5%. Furthermore, the frequency of genotypes and alleles in apoa5 -1131T>C and 56C>G polymorphisms was clearly different from other races and areas. We conclude that the apoa5 -1131T>C variation should be considered a single nucleotide polymorphism, but the 56C>G variation should be considered as a mutation instead.
- Published
- 2007