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[Objective] To establish methods to determine the content of Aconitine, Yunaconitine, Crassicauline and Vilmorrianine in Aconitun sungpanense Hand-Mazz. and provide scientific basis for its reasonable and safe application. [Methods] Aconitine, Yunaconitine, Crassicauline and Vilmorrianine were used as references. Phenomenex Gemini C18 110A (250 mm ×4.6 mm, 5 [μm); mobile phase; acetonitrile -0.2% acetic acid (pH was regulated to by triethylamine); gradient elute; flow rate; 1 mL/min; UV wavelength 235/254 nm; column temperature; 30 CC. [Results] The content of the 4 Ester-series alkaloids in Aconitun sungpanense Hand. -Mazz. could be determined at the same time. The average recovery rate (n = 6) of Aconitine, Yunaconitine, Crassicauline and Vilmorrianine reached 99. 65% (RSD = 1. 46%), 99. 13% (RSD = 1.32%), 98.13% (RSD =0.98%) and 98.66% (RSD = 1.67%). [Conclusions] The method was simple, accurate and had good repeatability. It could determine the content of Aconitine, Yunaconitine, Crassicauline and Vilmorrianine in Aconitun sungpanense Hand.-Mazz. at the same time. It also provided scientific basis for standardizing the processing of Aconitun sungpanense Hand. -Mazz. and its reasonable and safe application. [ABSTRACT FROM AUTHOR]

Abstract: Supercritical water gasification (SCWG) of wet biomass is a very promising technology for hydrogen energy and the utilization of biomass resources. Ni-based catalysts are effective in catalyzing SCWG of original biomass and organic compounds for hydrogen production. In this paper, hydrogen production by SCWG of glucose over alumina-supported nickel catalysts modified with Cu, Co and Sn was studied. The bimetallic Ni–M (M = Cu, Co and Sn) catalysts were prepared by a co-impregnation method and tested in an autoclave reactor at 673 K with a feedstock concentration of 9.09 wt.%. XRD, XRF, N2 adsorption/desorption, SEM and TGA were adopted to investigate the changes of chemical properties between Ni and Ni–M catalysts and the deactivation mechanism of catalysts. According to the experimental results, the hydrogen yield followed this order: Ni–Cu/γAl2O3 > Ni/γAl2O3 > Ni–Co/γAl2O3 > Ni–Sn/γAl2O3. The results show that Cu could improve the catalytic activity of Ni catalyst in reforming reaction of methane to produce hydrogen in SCWG. In addition, Cu can mitigate the sintering of alumina detected by SEM. Co was found to be an excellent promoter of Ni-based catalyst in relation to hydrogen selectivity. [Copyright &y& Elsevier]

Abstract: Hydrogen production by supercritical water gasification (SCWG) is a promising technology for wet biomass utilization. Ni catalyst can realize the high gasification efficiency of biomass near the critical temperature of water. In this paper, Ni/γAl2O3 and Ni/CeO2-γAl2O3 catalysts were prepared by an impregnation method. The catalyst performance for glucose gasification in supercritical water was tested in autoclave reactor. All experiments were carried out in the autoclave at 673 K, 24.5 MPa, and the concentration of glucose was 9.09 wt.%. The catalysts before and after reaction were characterized by X-ray diffraction (XRD), scanning electron microscopy (SEM), BET specific surface area measurements, X-ray fluorescence spectrum (XRF) and Thermo-gravimetric analyses (TGA) in order to investigate on the chemical property and catalytic mechanism. The experimental results showed that hydrogen yield and hydrogen selectivity increased sharply with addition of Ni/γAl2O3 and Ni/CeO2-γAl2O3 catalysts. The catalytic activity and H2 selectivity of Ni/CeO2-γAl2O3 was higher than that of Ni/γ-Al2O3 catalyst. The results revealed that carbon deposition and coking led to the deactivation of the catalysts. Ce in the Ni/CeO2-γAl2O3 catalyst had a certain role in the inhibition of carbon deposition and coking. [Copyright &y& Elsevier]

Floral development and senescence are a crucial determinant for economic and ornamental value. CircRNAs play an essential role in regulating plant growth and development; however, there is no systematic identification of circRNAs during the lifespan of flowers. This study aims to explore the expression profile and functional role of circRNAs in the full flowering stages of Rhododendron delavayi Franch . We carried out transcriptome sequencing of the six stages of Rhododendron delavayi Franch flowers to identify the circular RNA expression profile. In addition, using bioinformatics methods, we explored the functions of circRNAs, including analysis of the circRNA-miRNA-mRNA network, short time-series expression miner (STEM), and so on. We identified 146 circRNAs, of which 79 were differentially expressed from the budding to fading stages. Furthermore, using STEM analysis, one of the 42 circRNA expression model profiles was significantly upregulated during the senescence stage, including 16 circRNAs. Additionally, 7 circRNA-miRNA-mRNA networks were constructed with 10 differentially expressed circRNAs, in which some target mRNA may regulate the development and senescence of the Rhododendron flowers. Finally, by analyzing the correlation between circRNAs and mRNA, combined with existing reports, we proposed that circRNAs play a regulatory role during flower development and senescence by mediating the jasmonate signaling pathway. Overall, these results provide new clues to the potential mechanism of circRNAs acting as novel post-transcriptional regulators in the development and senescence process of flowers.

The present study aimed at systematically investigating the endophytic communities of dominant plants in the karst ecosystem. Soil and plant materials were collected and after sequencing of the 16 s RNA, the diversity and abundance of the endophytic community structures in leaves were examined. Our results showed that abundant and diverse endogenous bacteria were associated with the leaves of common dominant plants living in the karst ecological environment. Notably, common traits and significant differences in the endophytic community structures were recorded among different plant species with different leaf grown in soils with different calcium contents. These observations implied that plants may adopt different strategies to adapt to the karst ecological environment. In addition, the endophytic bacteria associated with the leaves may be involved in different physiological strategies used by the plants to adapt to the karst ecological environment. These findings provide new avenues for developing microbial agents that could be suitable for the karst ecological environment and will provide sustainable solutions for improving the ability of plants to adapt to karst special adversities, and thus for karst geomorphological environmental protection and agricultural development.

As a mono-sodium salt form of alendronic acid, alendronate sodium presents multi-level ionization for the dissociation of its four hydroxyl groups. The dissociation constants of alendronate sodium were determined in this work by studying the piecewise linear relationship between volume of titrant and pH value based on acid-base potentiometric titration reaction. The distribution curves of alendronate sodium were drawn according to the determined pKa values. There were 4 dissociation constants (pKa 1 =2.43, pKa 2 =7.55, pKa 3 =10.80, pKa 4 =11.99, respectively) of alendronate sodium, and 12 existing forms, of which 4 could be ignored, existing in different pH environments.

Objective: The aim of this study was to analyze the bacterial diversity of urban section of Nanming river as well as to study relationship between the bacterial diversity and environmental factors., Methods: The high throughput sequencing on PCR-amplified 16S rDNA V4 fragments was used to determine the bacterial diversity of samples from five sites of Nanming river. The ordination technique of redundancy analysis was used to analyze the effects of the environmental factors on bacterial community composition., Results: Diversity index analysis of bacterial community composition in Nanming river showed that the Shannon-Wiener index of bacterial diversity in Nanmng River was about 7.5. The Shannon-Wiener index of 5 sampling site had an order as Wudang bridge > Shuikou Si > Wuyan bridge > Huaxi bridge > Guanzhou bridge. Based on the sequencing results, there were 11 phyla (327 genera) in the samples, of which proteobacterice (66.1% ± 3.30% ) was the dominant phyla, Gamma proteobacteria (54.76% ± 4.86%) was the dominant subgroup and Pseudomonas (16.92% ± 0.02%) was the dominant genera. In addition, there are some flora and rare flora undetermined. The result of RDA suggested that the influence of different environmental factors on different microbes were different. Bacterial community IV had significant positive correlation with total nitrogen and total phosphorus in the environment., Conclusion: This research provides references for the association of bacterial composition and diversity with environmental factors.

Carney complex is a syndrome that may include cardiac and mucocutaneous myxomas, spotting skin pigmentation, and endocrine lesions. Many patients with Carney complex have been shown to have a stop codon mutation in the PRKAR1A gene in the 17q22-24 region. Here we present the case of a 57 year-old man with multiple skin lesions and cardiac myxomas. Histology of the skin lesions showed lentigenous melanocytic hyperplasia and cutaneous myxomas, confirming the diagnosis of Carney complex. Lesional and control normal tissue from the patient were identified and sequenced for the PRKAR1A gene. A germline missense mutation was identified at exon 1A. This is the first report of this mutation, and one of the few reported missense mutation associated with Carney complex. This finding strengthens the argument that there are alternative ways in which the protein kinase A 1-alpha subunit plays a role in tumorigenesis.