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The genes of colonization factor K88 and avirulent heat-labile enterotoxin LT A-B+ of enterotoxigenic E. coli (ETEC) have been ligated, and a vaccine strain containing recombinant plasmid that efficiently expresses two antigens has been obtained. Using the activity of beta-galactosidase as a selection marker instead of drug resistance, another bivalent vaccine strain with the same expression level has also been constructed. The vaccine strains have no toxicity and do not cause any adverse reactions. In challenge study and field trials, a high degree of protection from colibacillosis was afforded to piglets when their dams were immunized orally or parenterally. Practice of bivalent live vaccines including colonization factor and enterotoxin antigens and without antibiotic resistance gene shows effective.

Two cases of abdominal cocoon are reported. Abdominal cocoon is rare, only 31 cases reported in the literature. The majority of the cases are adolescent girls with symptoms of acute or chronic intestinal obstruction and/or abdominal mass. Ultrasound examination shows the mass is a shadow of intestinal loop aggregation and barium meal reveal the mass to be made of folded intestine. Operative findings showed the bowel is encased in a white, tough and dense membrane that on microscopic examination thickened fibrous tissue are found Partiac. Removal of the membrane, releasing the intestine adhesions and plication of the bowel may be effective.

Human peripheral blood lymphocytes were cultured in diffusion chamber in mice after short-term administration of gossypol acetate. The induced chromosomal aberrations of human target cells indiffusion chamber were used as indices of DNA damage. The results showed that the mean frequency of chromosomal aberration of the human lymphocytes in Gossypol-treated mice was slightly higher than that in the control group, but the difference between them was not statistically significant (p 0.05).

Gossypol acetic acid has been shown to have the effect of increasing the SCE frequencies in the spermatogonial cells of mice. This study further confirmed the genotoxic effects of gossypol acetic acid by investigating the dose-response relationships of gossypol acetic acid-induced SCEs in spermatogonial cells and bone marrow cells of mice. Twelve or over 12 mg/kg body weight of the dosage per day could increase the SCE frequencies of both bone marrow cells and spermatogonial cells. The increase of SCE frequency appeared to be directly correlated with the doses used at significant levels (p less than 0.001). Regression lines indicated that, in spite of cell type variation, the SCE induced was proportional to the dose of gossypol acetic acid.

Isolated mycobacterium from 219 cases of pulmonary tuberculosis was identified according to the criteria of growth in para-aminobenzoic acid medium, rough or smooth type of colony as well as easy or difficult to emulsified etc. 6 strains (2.7%) of atypical mycobacteria have been found. Among them 3 cases (1.4%) of pulmonary mycobacterial intracellular diseases were diagnosed finally. All of the 211 strains (cases) of the rough type are difficult to emulsify, but 8 strains (cases) of the smooth type are all easy to emulsify.