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Additional file 1: Figure S1. Gene structure and motif pattern of the Gh4CL genes. a Structural analysis. The left panel shows the neighbor-joining phylogenetic tree based on the amino acid sequences of the Gh4CLs. The classes I, II and III were marked correspondingly. The right panel shows the exon-intron structure of each Gh4CL gene with exons showing in orange boxes, introns in black lines between exons, and upstream/downstream UTRs in blue boxes. The number indicates the phase of the corresponding introns. The length of the Gh4CL genes is indicted by the scale line at the bottom. b Motif analysis. The motif analysis was performed by the MEME suite. Twenty motifs were detected and are displayed in color coded boxes. The length of proteins is indicted by the scale line at the bottom. Figure S2. Alignment of multiple Gh4CL and selected At4CL domain amino acid sequences. Multiple sequence alignment was performed using Clustal X. Box I and Box II represent the two conserved domains of the Gh4CL proteins. Figure S3. Analysis of cis-elements in the promoter of the Gh4CL genes. The numbers of different cis-elements are presented in the form of bar graphs. Figure S4. Relative expression levels of Gh4CL7 in plants infiltrated with TRV:00 and TRV:Gh4CL7 (n = 5). Total RNA was extracted from leaves at 2 weeks post-infiltration. Transcript levels were determined by qRT-PCR using GhUBQ7 as control. Figure S5. Silencing of the endogenous magnesium chelatase subunit I gene (GhCHLI) in cotton through VIGS. The leaf bleaching phenotype was observed 2 weeks after infiltration in TRV: GhCHLI plants. Figure S6. Transcription levels of stress responsive genes in CK and Gh4CL7-siliencing cotton plants. The normal condition plants were used as controls. GhUBQ7 gene was used as an internal control. All the gene expressions were normalized to the corresponding transcript levels in CK plants at normal condition. All the data represent mean ± SE for three biological replications. Figure S7. Transcription levels of stress responsive genes in transgenic and WT Arabidopsis plants. The normal condition plants were used as controls. AtEF-Lα gene was used as an internal control. All the gene expressions were normalized to the corresponding transcript levels in WT plants at normal condition. All the data represent mean ± SE for three biological replications.

Additional file 1 Table 1. Univariate Logistic Regression Analysis Between Macrosomia and the Maternal Clinical Parameters. Table 2. The mean and 95% CI of ISFHAC with BMI

Rationale: Micrognathia is a subtle facial malformation characterized by a small mandible and receding chin. Fetal micrognathia is often associated with chromosomal abnormalities, skeletal dysplasia, and various syndromes. Once it is dignosised, detailed fetal malformation screening and chromosome examination should be carried out. Patient concern: One pregnant woman with suspicion of fetal micrognathia was referred from her local hospital to our hospital for detailed fetal malformation screening and fetal echocardiography. Examination of the fetus was performed using a two-dimensional and three-dimensional ultrasound probe in multiple planes. The fetus showed micrognathia without glossoptosis with features of the inferior facial angle (IFA) ≤50° and his tongue reached anterior mandibular border box during normal movement. Diagnoses: The fetus was diagnosed as isolated micrognathia prenatally without multisystem abnormalities. Interventions: Amniocentesis was performed and the fetus was found to carry 46XN with 6q14.1 duplication, the significance of which was unclear. Outcomes: The fetus was labored through vagina at 38 weeks gestation. A small soft cleft palate was diagnosed after delivery. Lessons: This case suggests that once prenatal diagnosis of the fetal micrognathia has been made, we should carefully examine the presence of fetus's multisystem developmental abnormalities and due consideration should be given for associated soft cleft palate.

Additional file 2: Table S1. Ka, Ks, Ka/Ks values for the Gh4CL paralogous gene pairs. Table S2. Information of the 20 motifs of Gh4CL proteins. Table S3. The primers used in qRT-PCR analysis.