301. Double labelling of intracellular mitochondria and nucleolus using thiophene pyridium salt with high quantum yield as biosensor and its application in stimulated emission depletion nanoscopy
- Author
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Yingzhong Zhu, Xiaohe Tian, Jieying Wu, Qiong Zhang, Yan Chen, Yupeng Tian, Mingzhu Zhang, and Hui Wang
- Subjects
Membrane permeability ,Nucleolus ,Pyridines ,Quantum yield ,02 engineering and technology ,Biosensing Techniques ,Thiophenes ,Molecular Dynamics Simulation ,010402 general chemistry ,01 natural sciences ,Biochemistry ,Analytical Chemistry ,Small Molecule Libraries ,chemistry.chemical_compound ,medicine ,Thiophene ,Environmental Chemistry ,Humans ,Nanotechnology ,Spectroscopy ,Fluorescent Dyes ,Molecular Structure ,STED microscopy ,Hep G2 Cells ,021001 nanoscience & nanotechnology ,0104 chemical sciences ,Mitochondria ,Cytosol ,medicine.anatomical_structure ,chemistry ,Microscopy, Fluorescence ,Biophysics ,Quantum Theory ,0210 nano-technology ,Biosensor ,Nucleus ,Cell Nucleolus - Abstract
Probe for dual-site target distinct subcellular compartments from cytosol and nucleus is an attractive approach, however, which was scarcely reported. Herein, a series of small-molecular thiophene pyridium salt derivatives (MitoNuc1-4) possessing water-soluble, high quantum yield and two-photon activity were rationally designed, and their structures were crystallographic confirmed. Systematic photophysical and biological imaging property investigations were carried out for them. It was found that MitoNuc1-4 exhibit two-photon absorption properties in the near infrared region, and MitoNuc1 has membrane permeability and cationic nature, rendering it to be double labelling of mitochondria and nucleolus in living cells with superb photo-stability and non-invasiveness. It also demonstrated that MitoNuc1 in living cells can monitor mitochondrial division in real time and revealed nucleolar ultrastructure under stimulated emission depletion nanoscopy.
- Published
- 2017