151. G-protein-coupled receptor phosphorylation and palmitoylation.
- Author
-
Tobin AB and Wheatley M
- Subjects
- Animals, CHO Cells, Cell Membrane metabolism, Cricetinae, Palmitic Acid metabolism, Phosphorus Radioisotopes metabolism, Phosphorylation, Precipitin Tests methods, Receptor, Muscarinic M3 metabolism, Tritium metabolism, Protein Processing, Post-Translational physiology, Receptors, G-Protein-Coupled metabolism
- Abstract
It is now clear that nearly all G-protein-coupled receptors (GPCRs) are phosphorylated and palmitolyated. The process of receptor phosphorylation has been extensively studied because it offers a regulatory mechanism that is both rapid and dynamic. However, it has recently become clear that palmitoyaltion of GPCRs at C-terminal cysteine residues may also offer dynamic receptor modification. A growing number of GPCRs have been demonstrated to undergo rapid agonist-mediated changes in their palmitoylation status with functional implications to receptor signaling. This chapter aims to outline the methods we have used to investigate agonist-mediated changes in GPCR phosphorylation and palmitoylation.
- Published
- 2004
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