251. Characterization of enterotoxin produced by four Yersinia enterocolitica strains of pig origin.
- Author
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Verma NK and Misra DS
- Subjects
- Animals, Capillary Permeability, Enterotoxins isolation & purification, Hydrogen-Ion Concentration, Ileum, Methanol, Mice, Rabbits, Swine microbiology, Temperature, Yersinia isolation & purification, Yersinia metabolism, Yersinia enterocolitica isolation & purification, Enterotoxins biosynthesis, Yersinia enterocolitica metabolism
- Abstract
All four isolates of Yersinia enterocolitica and one isolate of Y. frederiksenii from pigs were found to be enterotoxigenic. Whole-cell preparations of Y. enterocolitica isolates did not induce any change in the rabbit ligated gut test after 6 and 18 h of inoculation, but Y. frederiksenii on the other hand showed a positive gut response at 18 h. Cell-free supernatant (CFS) of all five isolates induced dilatation in the rabbit gut up to 6 h, after which Y. enterocolitica became negative, while Y. frederiksenii continued to show a reaction up to 18 h. CFS of all five isolates were also found positive with the infant mouse test. Of the five isolates of Yersinia, three gave a positive reaction for the permeability factor on rabbit skin. Yersinia enterotoxin could be concentrated by methanol extraction. It was stable at 100 degrees C for 20 min and at 120 degrees C for 15 min. However, its activity was lost at low (2.0) and high pH (10.0). Enterotoxic preparations of Y. enterocolitica lost part of their enterotoxic activity upon dialysis.
- Published
- 1984
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