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308. CATION EXCHANGER1 Cosegregates with Cadmium Tolerance in the Metal Hyperaccumulator Arabidopsis halleri and Plays a Role in Limiting Oxidative Stress in Arabidopsis Spp.

Author

Baliardini, Cecilia, Meyer, Claire-Lise, Salis, Pietrino, Saumitou-Laprade, Pierre, and Verbruggen, Nathalie

Subjects
ARABIDOPSIS halleri, PLANT adaptation, EFFECT of cadmium on plants, ADENOSINE triphosphatase, LOCUS in plant genetics
Abstract

Arabidopsis halleri is a model species for the study of plant adaptation to extreme metallic conditions. In this species, cadmium (Cd) tolerance seems to be constitutive, and the mechanisms underlying the trait are still poorly understood. A previous quantitative trait loci (QTL) analysis performed on A. halleri × Arabidopsis lyrata backcross population1 identified the metal-pump gene Heavy Metal ATPase4 as the major genetic determinant for Cd tolerance. However, although necessary, Heavy Metal ATPase4 alone is not sufficient for determining this trait. After fine mapping, a gene encoding a calcium2+/hydrogen+ antiporter, cation/hydrogen+ exchanger1 (CAX1), was identified as a candidate gene for the second QTL of Cd tolerance in A. halleri. Backcross population1 individuals displaying the A. halleri allele for the CAX1 locus exhibited significantly higher CAX1 expression levels compared with the ones with the A. lyrata allele, and a positive correlation between CAX1 expression and Cd tolerance was observed. Here, we show that this QTL is conditional and that it is only detectable at low external Ca concentration. CAX1 expression in both roots and shoots was higher in A. halleri than in the close Cd-sensitive relative species A. lyrata and Arabidopsis thaliana. Moreover, CAX1 loss of function in A. thaliana led to higher Cd sensitivity at low concentration of Ca, higher sensitivity to methylviologen, and stronger accumulation of reactive oxygen species after Cd treatment. Overall, this study identifies a unique genetic determinant of Cd tolerance in the metal hyperaccumulator A. halleri and offers a new twist for the function of CAX1 in plants. [ABSTRACT FROM AUTHOR]

Published
2015
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309. Evidence of various mechanisms of Cd sequestration in the hyperaccumulator Arabidopsis halleri, the non-accumulator Arabidopsis lyrata, and their progenies by combined synchrotron-based techniques.

Author

Isaure, Marie-Pierre, Huguet, Stéphanie, Meyer, Claire-Lise, Castillo-Michel, Hiram, Testemale, Denis, Vantelon, Delphine, Saumitou-Laprade, Pierre, Verbruggen, Nathalie, and Sarret, Géraldine

Subjects
CADMIUM content of plants, CHEMICAL composition of plants, ARABIDOPSIS halleri, ARABIDOPSIS lyrata, PLANT genetics
Abstract

Arabidopsis halleri is a model plant for Zn and Cd hyperaccumulation. The objective of this study was to determine the relationship between the chemical forms of Cd, its distribution in leaves, and Cd accumulation and tolerance. An interspecific cross was carried out between A. halleri and the non-tolerant and non-hyperaccumulating relative A. lyrata providing progenies segregating for Cd tolerance and accumulation. Cd speciation and distribution were investigated using X-ray absorption spectroscopy and microfocused X-ray fluorescence. In A. lyrata and non-tolerant progenies, Cd was coordinated by S atoms only or with a small contribution of O groups. Interestingly, the proportion of O ligands increased in A. halleri and tolerant progenies, and they were predominant in most of them, while S ligands were still present. Therefore, the binding of Cd with O ligands was associated with Cd tolerance. In A. halleri, Cd was mainly located in the xylem, phloem, and mesophyll tissue, suggesting a reallocation process for Cd within the plant. The distribution of the metal at the cell level was further discussed. In A. lyrata, the vascular bundles were also Cd enriched, but the epidermis was richer in Cd as compared with the mesophyll. Cd was identified in trichomes of both species. This work demonstrated that both Cd speciation and localization were related to the tolerance character of the plant. [ABSTRACT FROM AUTHOR]

Published
2015
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310. Intraspecific variability of cadmium tolerance and accumulation, and cadmium-induced cell wall modifications in the metal hyperaccumulator Arabidopsis halleri.

Author

Meyer, Claire-Lise, Juraniec, Michal, Huguet, Stéphanie, Chaves-Rodriguez, Elena, Salis, Pietro, Isaure, Marie-Pierre, Goormaghtigh, Erik, and Verbruggen, Nathalie

Subjects
CADMIUM content of plants, PLANT cell walls, CHEMICAL composition of plants, PLANT cells & tissues, ARABIDOPSIS halleri
Abstract

Certain molecular mechanisms of Cd tolerance and accumulation have been identified in the model species Arabidopsis halleri, while intraspecific variability of these traits and the mechanisms of shoot detoxification were little addressed. The Cd tolerance and accumulation of metallicolous and non-metallicolous A. halleri populations from different genetic units were tested in controlled conditions. In addition, changes in shoot cell wall composition were investigated using Fourier transform infrared spectroscopy. Indeed, recent works on A. halleri suggest Cd sequestration both inside cells and in the cell wall/apoplast. All A. halleri populations tested were hypertolerant to Cd, and the metallicolous populations were on average the most tolerant. Accumulation was highly variable between and within populations, and populations that were non-accumulators of Cd were identified. The effect of Cd on the cell wall composition was quite similar in the sensitive species A. lyrata and in A. halleri individuals; the pectin/polysaccharide content of cell walls seems to increase after Cd treatment. Nevertheless, the changes induced by Cd were more pronounced in the less tolerant individuals, leading to a correlation between the level of tolerance and the extent of modifications. This work demonstrated that Cd tolerance and accumulation are highly variable traits in A. halleri, suggesting adaptation at the local scale and involvement of various molecular mechanisms. While in non-metallicolous populations drastic modifications of the cell wall occur due to higher Cd toxicity and/or Cd immobilization in this compartment, the increased tolerance of metallicolous populations probably involves other mechanisms such as vacuolar sequestration. [ABSTRACT FROM AUTHOR]

Published
2015
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311. NaCl and CuSO4 treatments trigger distinct oxidative defence mechanisms in Nicotiana plumbaginifolia L.

Author

Savouré, Arnould, Thorin, D., Davey, Mark, Hua, Xue-Jun, Mauro, Serge, Van Montagu, Marc, Inzé, Dirk, Verbruggen, Nathalie, Savouré, Arnould, Thorin, D., Davey, Mark, Hua, Xue-Jun, Mauro, Serge, Van Montagu, Marc, Inzé, Dirk, and Verbruggen, Nathalie

Abstract

To gain better insights into the generation of oxidative stress by NaCl stress, we compared the effects of toxic, sublethal concentrations of NaCl (150 mM) with those of CuSO4 (100 μM) on the antioxidant defence responses of Nicotiana plumbaginifolia L. plants grown in hydroponic culture. Short- and long-term adaptive responses were investigated as well as the effects of root cutting to allow rapid absorption of ions. Our results show that photosynthesis is already affected after 12 h of both treatments and that photoinhibition is induced. Furthermore, the two treatments showed distinct patterns of induction of the genes of the antioxidant systems. Principally, NaCl stimulated catalase activity through activation of the Cat2 and Cat3 genes, whereas with CuSO4, transcript levels, protein levels, and enzymatic activities of glutathione peroxidase, ascorbate peroxidase, and superoxide dismutase were differentially induced. The antioxidants ascorbate and glutathione were found to be largely oxidized after 12 h, suggesting an inability of the recycling process to cope with the stress. In addition, both NaCl and CuSO4 treatments stimulated the accumulation of the osmolyte proline. Therefore, we conclude that the uptake and accumulation of different types of ions lead to the generation of distinct antioxidant responses in N. plumbaginifolia., SCOPUS: ar.j, FLWIN, info:eu-repo/semantics/published

Published
1999

312. Characterization of an Arabidopsis thaliana receptor-like protein kinase gene activated by oxidative stress and pathogen attack

Author

Czernic, Pierre, Visser, Botma, Savouré, Arnould, Van Montagu, Marc, Verbruggen, Nathalie, Czernic, Pierre, Visser, Botma, Savouré, Arnould, Van Montagu, Marc, and Verbruggen, Nathalie

Abstract

An Arabidopsis thaliana cDNA clone that encodes a putative receptor-like protein kinase gene (At-RLK3) was characterized. The deduced 667-amino acid protein consists of an amino-terminal signal sequence, an extracellular domain, a single transmembrane domain, and a cytoplasmic domain with characteristics of serine/threonine protein kinase. Because of the original features of its extracellular domain, the At-RLK3 protein is a member of a new class of receptor-like protein kinases. The At-RLK3 gene is present as a single copy within the Arabidopsis genome and its transcripts are detected in root, stem, leaf and flower. In cultured cells, the At-RLK3 gene is activated upon oxidative stress and salicylic acid treatment. In plants, the gene appears to be differentially regulated during various plant–pathogen interactions: upon inoculation with strains of Pseudomonas syringae pv. tomato harboring or not, different avr genes, At-RLK3 transcripts accumulate transiently at similar levels during both compatible and incompatible interactions. This gene is, however, preferentially expressed during the incompatible interaction induced by the soil-borne vascular bacteria, Ralstonia solanacearum. The involvement of At-RLK3 in signal transduction pathways during pathogen attack is discussed., FLWIN, info:eu-repo/semantics/published

Published
1999

315. UBR2 can suppress the salt sensitivity of the sas1 yeast mutant

Author

Sun, Weining, Lee, Jeong Hee, Czernic, Pierre, Savouré, Arnould, Van Montagu, Marc, Verbruggen, Nathalie, Sun, Weining, Lee, Jeong Hee, Czernic, Pierre, Savouré, Arnould, Van Montagu, Marc, and Verbruggen, Nathalie

Abstract

info:eu-repo/semantics/published

Published
1999

318. Plant responses to osmotic stress

Author

Verbruggen, Nathalie, Van Montagu, Marc, Verbruggen, Nathalie, and Van Montagu, Marc

Abstract

info:eu-repo/semantics/published

Published
1999

330. Looking for the roles of proline dehydrogenase in Arabidopsis thaliana

Author

Mani, Srikrishnan, Messens, Joris, Van de Cotte, Brigitte, Hua, Xue-Jun, Czernic, Pierre, Van Montagu, Marc, Verbruggen, Nathalie, Mani, Srikrishnan, Messens, Joris, Van de Cotte, Brigitte, Hua, Xue-Jun, Czernic, Pierre, Van Montagu, Marc, and Verbruggen, Nathalie

Abstract

info:eu-repo/semantics/published

Published
1998

332. Study of a salt sensitive yeast mutant

Author

Sun, Weining, Lee, Jeong Hee, Czernic, Pierre, Savouré, Arnould, Van Montagu, Marc, Verbruggen, Nathalie, Sun, Weining, Lee, Jeong Hee, Czernic, Pierre, Savouré, Arnould, Van Montagu, Marc, and Verbruggen, Nathalie

Abstract

info:eu-repo/semantics/published

Published
1998

334. Etat de la recherche en Belgique

Author

colloque organisé par la commission économie du conseil des Femmes Francophones de Belgique, Verbruggen, Nathalie, colloque organisé par la commission économie du conseil des Femmes Francophones de Belgique, and Verbruggen, Nathalie

Abstract

info:eu-repo/semantics/published

Published
1998

335. Témoignage

Author

Verbruggen, Nathalie and Verbruggen, Nathalie

Abstract

Actes du Colloque organisé par la Commission Economie, info:eu-repo/semantics/published

Published
1998

336. How to increase osmotolerance in crops?

Author

Verbruggen, Nathalie, Van Montagu, Marc, Verbruggen, Nathalie, and Van Montagu, Marc

Abstract

info:eu-repo/semantics/published

Published
1998

338. Arabidopsis Responses to Salt Stress

Author

Verbruggen, Nathalie, Van Montagu, Marc, Verbruggen, Nathalie, and Van Montagu, Marc

Abstract

info:eu-repo/semantics/published

Published
1998

339. Cell Cycle in Plants

Author

Verbruggen, Nathalie, Van Montagu, Marc, Verbruggen, Nathalie, and Van Montagu, Marc

Abstract

info:eu-repo/semantics/published

Published
1998

343. Proline oxidation is post-translationnally regulated

Author

Verbruggen, Nathalie, Messens, Joris, Van de Cotte, Brigitte, Van Montagu, Marc, Verbruggen, Nathalie, Messens, Joris, Van de Cotte, Brigitte, and Van Montagu, Marc

Abstract

info:eu-repo/semantics/published

Published
1997

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