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In April 2020, the QUality Assessment and REProducibility for Instruments and Images in Light Microscopy (QUAREP-LiMi) initiative was formed. This initiative comprises imaging scientists from academia and industry who share a common interest in achieving a better understanding of the performance and limitations of microscopes and improved quality control (QC) in light microscopy. The ultimate goal of the QUAREP-LiMi initiative is to establish a set of common QC standards, guidelines, metadata models, and tools, including detailed protocols, with the ultimate aim of improving reproducible advances in scientific research. This White Paper 1) summarizes the major obstacles identified in the field that motivated the launch of the QUAREP-LiMi initiative; 2) identifies the urgent need to address these obstacles in a grassroots manner, through a community of stakeholders including, researchers, imaging scientists, bioimage analysts, bioimage informatics developers, corporate partners, funding agencies, standards organizations, scientific publishers, and observers of such; 3) outlines the current actions of the QUAREP-LiMi initiative, and 4) proposes future steps that can be taken to improve the dissemination and acceptance of the proposed guidelines to manage QC. To summarize, the principal goal of the QUAREP-LiMi initiative is to improve the overall quality and reproducibility of light microscope image data by introducing broadly accepted standard practices and accurately captured image data metrics., Comment: 17 pages, 3 figures, shortened abstract, Co-Lead Authors: Glyn Nelson and Ulrike Boehm, Corresponding author: Roland Nitschke

Observations of metal absorption systems in the spectra of distant quasars allow to constrain a possible variation of the fine-structure constant throughout the history of the Universe. Such a test poses utmost demands on the wavelength accuracy and previous studies were limited by systematics in the spectrograph wavelength calibration. A substantial advance in the field is therefore expected from the new ultra-stable high-resolution spectrograph Espresso, recently installed at the VLT. In preparation of the fundamental physics related part of the Espresso GTO program, we present a thorough assessment of the Espresso wavelength accuracy and identify possible systematics at each of the different steps involved in the wavelength calibration process. Most importantly, we compare the default wavelength solution, based on the combination of Thorium-Argon arc lamp spectra and a Fabry-P\'erot interferometer, to the fully independent calibration obtained from a laser frequency comb. We find wavelength-dependent discrepancies of up to 24m/s. This substantially exceeds the photon noise and highlights the presence of different sources of systematics, which we characterize in detail as part of this study. Nevertheless, our study demonstrates the outstanding accuracy of Espresso with respect to previously used spectrographs and we show that constraints of a relative change of the fine-structure constant at the $10^{-6}$ level can be obtained with Espresso without being limited by wavelength calibration systematics., Comment: 27 pages, accepted for publication in A&A

From soda cans to space rockets, thin-walled cylindrical shells are abundant, offering exceptional load carrying capacity at relatively low weight. However, the actual load at which any shell buckles and collapses is very sensitive to imperceptible defects and can not be predicted, which challenges the reliable design of such structures. Consequently, probabilistic descriptions in terms of empirical design rules are used and reliable design requires to be very conservative. We introduce a nonlinear description where finite-amplitude perturbations trigger buckling. Drawing from the analogy between imperfect shells which buckle and imperfect pipe flow which becomes turbulent, we experimentally show that lateral probing of cylindrical shells reveals their strength non-destructively. A new ridge-tracking method is applied to commercial cylinders with a hole showing that when the location where buckling is nucleated is known we can accurately predict the buckling load of each individual shell, within $\pm 5\%$. Our study provides a new promising framework to understand shell buckling, and more generally, imperfection-sensitive instabilities.

Dynamic assessment of mortality risk in the intensive care unit (ICU) can be used to stratify patients, inform about treatment effectiveness or serve as part of an early-warning system. Static risk scoring systems, such as APACHE or SAPS, have recently been supplemented with data-driven approaches that track the dynamic mortality risk over time. Recent works have focused on enhancing the information delivered to clinicians even further by producing full survival distributions instead of point predictions or fixed horizon risks. In this work, we propose a non-parametric ensemble model, Weighted Resolution Survival Ensemble (WRSE), tailored to estimate such dynamic individual survival distributions. Inspired by the simplicity and robustness of ensemble methods, the proposed approach combines a set of binary classifiers spaced according to a decay function reflecting the relevance of short-term mortality predictions. Models and baselines are evaluated under weighted calibration and discrimination metrics for individual survival distributions which closely reflect the utility of a model in ICU practice. We show competitive results with state-of-the-art probabilistic models, while greatly reducing training time by factors of 2-9x., Comment: 9 pages, 6 figures

The Quantizer problem is a tessellation optimisation problem where point configurations are identified such that the Voronoi cells minimise the second moment of the volume distribution. While the ground state (optimal state) in 3D is almost certainly the body-centered cubic lattice, disordered and effectively hyperuniform states with energies very close to the ground state exist that result as stable states in an evolution through the geometric Lloyd's algorithm [Klatt et al. Nat. Commun., 10, 811 (2019)]. When considered as a statistical mechanics problem at finite temperature, the same system has been termed the 'Voronoi Liquid' by [Ruscher et al. EPL 112, 66003 (2015)]. Here we investigate the cooling behaviour of the Voronoi liquid with a particular view to the stability of the effectively hyperuniform disordered state. As a confirmation of the results by Ruscher et al., we observe, by both molecular dynamics and Monte Carlo simulations, that upon slow quasi-static equilibrium cooling, the Voronoi liquid crystallises from a disordered configuration into the body-centered cubic configuration. By contrast, upon sufficiently fast non-equilibrium cooling (and not just in the limit of a maximally fast quench) the Voronoi liquid adopts similar states as the effectively hyperuniform inherent structures identified by Klatt et al. and prevents the ordering transition into a BCC ordered structure. This result is in line with the geometric intuition that the geometric Lloyd's algorithm corresponds to a type of fast quench., Comment: 11 pages, 6 figures

Van der Waals heterostructures provide a rich platform for emergent physics due to their tunable hybridization of electronic orbital- and spin-degrees of freedom. Here, we show that a heterostructure formed by twisted bilayer graphene sandwiched between ferromagnetic insulators develops flat bands stemming from the interplay between twist, exchange proximity and spin-orbit coupling. We demonstrate that in this flat-band regime, the spin degree of freedom is hybridized, giving rise to an effective triangular superlattice with valley as a degenerate pseudospin degree of freedom. Incorporating electronic interactions at half-filling leads to a spontaneous valley-mixed state, i.e., a correlated state in the valley sector with geometric frustration of the valley spinor. We show that an electric interlayer bias generates an artificial valley-orbit coupling in the effective model, controlling both the valley anisotropy and the microscopic details of the correlated state, with both phenomena understood in terms of a valley-Heisenberg model with easy-plane anisotropic exchange. Our results put forward twisted graphene encapsulated between magnetic van der Waals heterostructures as platforms to explore purely valley-correlated states in graphene., Comment: 6 pages, 4 figures and supplementary material (3 pages, 1 figure). Comments are welcome!

We combine theory, numerical simulation and experiments to investigate the breakup of two identical droplets entering a Y-junction with controlled spatial offset by which the second droplet trails the first. Based on fully resolved 3D simulations, we describe the flow physics leading to breakup. Scaling arguments, numerical simulation and experiments consistently show that for small initial offset, breakup always occurs with the droplet fragment volume depending linearly on the offset. Above a critical offset, which increases with the capillary number, the droplets enter the constriction sequentially without breakup. Our results are relevant for understanding the breakup behavior in a dense emulsion flowing through a linearly converging channel leading to a constriction. Such geometry is commonly used for the serial interrogation of droplet content in droplet microfluidic applications, where droplet breakup can limit the maximum throughput for such process. For capillary numbers up to $Ca\simeq10^{-2}$, the results from the two-droplet system in a Y-junction are consistent with breakup observations in dense emulsions flowing through a linearly converging channel. The deterministic relation between initial offset and resulting breakup in the two-droplet system suggests that the stochasticity that is observed in the breakup of a dense emulsion arises from multi-droplet interactions. The numerical value of the prefactor in the linear relation between initial offset and droplet fragment volume determined from experiments differs slightly from the one extracted from fully resolved numerical simulations. This discrepancy suggests that even at very high bulk surfactant concentrations, the rate-limiting surfactant adsorption kinetics allows for Marangoni stresses to develop and modify the droplet dynamics.

Viral transmission pathways have profound implications for public safety; it is thus imperative to establish a complete understanding of viable infectious avenues. Mounting evidence suggests SARS-CoV-2 can be transmitted via the air; however, this has not yet been demonstrated. Here we quantitatively analyze virion accumulation by accounting for aerosolized virion emission and destabilization. Reported superspreading events analyzed within this framework point towards aerosol mediated transmission of SARS-CoV-2. Virion exposure calculated for these events is found to trace out a single value, suggesting a universal minimum infective dose (MID) via aerosol that is comparable to the MIDs measured for other respiratory viruses; thus, the consistent infectious exposure levels and their commensurability to known aerosol-MIDs establishes the plausibility of aerosol transmission of SARS-CoV-2. Using filtration at a rate exceeding the destabilization rate of aerosolized SARS-CoV-2 can reduce exposure below this infective dose., Comment: 6 pages, 4 figures

Chaotic dynamics in systems ranging from low-dimensional nonlinear differential equations to high-dimensional spatio-temporal systems including fluid turbulence is supported by non-chaotic, exactly recurring time-periodic solutions of the governing equations. These unstable periodic orbits capture key features of the turbulent dynamics and sufficiently large sets of orbits promise a framework to predict the statistics of the chaotic flow. Computing periodic orbits for high-dimensional spatio-temporally chaotic systems remains challenging as known methods either show poor convergence properties because they are based on time-marching of a chaotic system causing exponential error amplification; or they require constructing Jacobian matrices which is prohibitively expensive. We propose a new matrix-free method that is unaffected by exponential error amplification, is globally convergent and can be applied to high-dimensional systems. The adjoint-based variational method constructs an initial value problem in the space of closed loops such that periodic orbits are attracting fixed points for the loop-dynamics. We introduce the method for general autonomous systems. An implementation for the one-dimensional Kuramoto-Sivashinsky equation demonstrates the robust convergence of periodic orbits underlying spatio-temporal chaos. Convergence does not require accurate initial guesses and is independent of the period of the respective orbit.

Large-scale motions, also known as superstructures, are dynamically relevant coherent structures in a wall-bounded turbulent flow, that span the entire domain in wall-normal direction and significantly contribute to the global energy and momentum transport. Recent investigations in channel and Couette flow, suggest that these large-scale motions are self-sustained, implying they are not driven by small-scale motions at the wall. Whether large-scale motions are self-sustained has however not yet been answered for open boundary layers, which are relevant for many applications. Here, using the asymptotic suction boundary layer flow at the friction Reynolds number $Re_\tau = 1\,168$ as a testbed, we show that large-scale motions are self-sustained also in boundary layers. Together with the previous investigations in confined flows, this observation provides strong evidence of the robust and general nature of coherent self-sustained processes in turbulent wall-bounded flows. The dynamics of the large-scale self-sustained process involving the growth, breakdown and regeneration of quasi-streamwise coherent streaks and vortices within the boundary layer shows temporal phase relations reminiscent of bursting as observed in buffer layer structures. The dynamics however differs from the quasi-periodic large-scale streak-vortex regeneration cycle observed in confined flows. Based on the similarity of the dynamics of large-scale motions in boundary layers and of small-scale buffer layer structures, we conjecture that the bursting behaviour is associated with the dynamical relevance of only one wall, while two confining walls lead to the quasi-periodic cycle.

A specific family of spanwise-localised invariant solutions of plane Couette flow exhibits homoclinic snaking, a process by which spatially localised invariant solutions of a nonlinear partial differential equation smoothly grow additional structure at their fronts while undergoing a sequence of saddle-node bifurcations. Homoclinic snaking is well understood in the context of simpler pattern forming systems such as the one-dimensional Swift-Hohenberg equation with cubic-quintic nonlinearity, whose solutions remarkably well resemble the snaking solutions of plane Couette flow. We study the structural stability of the characteristic snakes-and-ladders structure associated with homoclinic snaking for flow modifications that break symmetries of plane Couette flow. We demonstrate that wall-normal suction modifies the bifurcation structure of three-dimensional plane Couette solutions in the same way, a symmetry-breaking quadratic term modifies solutions of the one-dimensional Swift-Hohenberg equation. These modifications are related to the breaking of the discrete rotational symmetry. At large amplitudes of the symmetry-breaking wall-normal suction the connected snakes-and-ladders structure is destroyed. Previously unknown solution branches are created and can be parametrically continued to vanishing suction. This yields new localised solutions of plane Couette flow that exist in a wide range of Reynolds number.

Metallic open cell foams have multiple applications in industry, e. g. as catalyst supports in chemical processes. Their regular or heterogeneous microscopic structure determines the macroscopic thermodynamic and chemical properties. We present an object-oriented python library that generates state space models for simulation and control from the microscopic foam data, which can be imported from the image processing tool iMorph. The foam topology and the 3D geometric data are the basis for discrete modeling of the balance laws using the cell method. While the material structure imposes a primal chain complex to define discrete thermodynamic driving forces, the internal energy balance is evaluated on a second chain complex, which is constructed by topological duality. The heat exchange between the solid and the fluid phase is described based on the available surface data. We illustrate in detail the construction of the dual chain complexes, and we show how the structured discrete model directly maps to the software objects of the python code. As a test case, we present simulation results for a foam with a Kelvin cell structure, and compare them to a surrogate finite element model with homogeneous parameters.

Spatio-temporally chaotic dynamics of transitional plane Couette flow may give rise to regular turbulent-laminar stripe patterns with a large-scale pattern wavelength and an oblique orientation relative to the laminar flow direction. A recent dynamical systems analysis of the oblique stripe pattern demonstrated that the Navier-Stokes equations have unstable equilibrium solutions that capture the three-dimensional spatial structure of the oblique stripe patterns. Here we identify unstable periodic orbits that not only show oblique large-scale amplitude modulation but also have a characteristic time-evolution, unlike steady equilibrium solutions. The periodic orbits represent standing waves that slowly propagate across wavy velocity streaks in the flow on viscous diffusion time scales. The unstable periodic orbits are embedded in the edge of chaos in a symmetry subspace of plane Couette flow and thereby may mediate transition to and from turbulent flows with oblique patterns.

At sufficiently high Reynolds numbers, shear-flow turbulence close to a wall acquires universal properties. When length and velocity are rescaled by appropriate characteristic scales of the turbulent flow and thereby measured in \emph{inner units}, the statistical properties of the flow become independent of the Reynolds number. We demonstrate the existence of a wall-attached non-chaotic exact invariant solution of the fully nonlinear 3D Navier-Stokes equations for a parallel boundary layer that captures the characteristic self-similar scaling of near-wall turbulent structures. The branch of travelling wave solutions can be followed up to $Re=1,000,000$. Combined theoretical and numerical evidence suggests that the solution is asymptotically self-similar and exactly scales in inner units for Reynolds numbers tending to infinity. Demonstrating the existence of invariant solutions that capture the self-similar scaling properties of turbulence in the near-wall region is a step towards extending the dynamical systems approach to turbulence from the transitional regime to fully developed boundary layers.

Convection in a layer inclined against gravity is a thermally driven non-equilibrium system, in which both buoyancy and shear forces drive spatio-temporally complex flow. As a function of the strength of thermal driving and the angle of inclination, a multitude of convection patterns is observed in experiments and numerical simulations. Several observed patterns have been linked to exact invariant states of the fully nonlinear 3D Oberbeck-Boussinesq equations. These exact equilibria, traveling waves and periodic orbits reside in state space and, depending on their stability properties, are transiently visited by the dynamics or act as attractors. To explain the dependence of observed convection patterns on control parameters, we study the parameter dependence of the state space structure. Specifically, we identify the bifurcations that modify the existence, stability and connectivity of invariant states. We numerically continue exact invariant states underlying spatially periodic convection patterns at Pr=1.07 under changing control parameters for temperature difference between the walls and inclination angle. The resulting state branches cover various inclinations from horizontal layer convection to vertical layer convection and beyond. The collection of all computed branches represents an extensive bifurcation network connecting 16 different invariant states across control parameter values. Individual bifurcation structures are discussed in detail and related to the observed complex dynamics of individual convection patterns. Together, the bifurcations and associated state branches indicate at what control parameter values which invariant states coexist. This provides a nonlinear framework to explain the multitude of complex flow dynamics arising in inclined layer convection.

Thermal convection in an inclined layer between two parallel walls kept at different fixed temperatures is studied for fixed Prandtl number Pr=1.07. Depending on the angle of inclination and the imposed temperature difference, the flow exhibits a large variety of self-organized spatio-temporal convection patterns. Close to onset, these patterns have been explained in terms of linear stability analysis of primary and secondary flow states. At larger temperature difference, far beyond onset, experiments and simulations show complex, dynamically evolving patterns that are not described by stability analysis and remain to be explained. Here we employ a dynamical systems approach. We construct stable and unstable exact invariant states, including equilibria and periodic orbits of the fully nonlinear three-dimensional Oberbeck-Boussinesq equations. These invariant states underlie the observed convection patterns beyond their onset. We identify state-space trajectories that, starting from the unstable laminar flow, follow a sequence of dynamical connections between unstable invariant states until the dynamics approaches a stable attractor. Together, the network of dynamically connected invariant states mediates temporal transitions between coexisting invariant states and thereby supports the observed complex time-dependent dynamics in inclined layer convection.

Confinement or geometric frustration is known to alter the structure of soft matter, including copolymeric melts, and can consequently be used to tune structure and properties. Here we investigate the self-assembly of ABC and ABB 3-miktoarm star copolymers confined to a shell using coarse-grained Dissipative Particle Dynamics simulations. In bulk and flat geometries the ABC stars form hexagonal tilings, but this is topologically prohibited in a spherical geometry which normally is alleviated by forming pentagonal tiles. However, the molecular architecture of the ABC stars implies an additional 'color constraint' which only allows even tilings (where all polygons have an even number of edges) and we study the effect of these simultaneous constraints. We find that both ABC and ABB systems form spherical tiling patterns, the type of which depends on the radius of the spherical substrate. For small spherical substrates, all solutions correspond to patterns solving the Thomson problem of placing mobile repulsive electric charges on a sphere. In ABC systems we find three coexisting, possibly different tilings, one in each color, each of them solving the Thomson problem simultaneously. For all except the smallest substrates, we find competing solutions with seemingly degenerate free energies that occur with different probabilities. Statistically, an observer who is blind to the differences between B and C can tell from the structure of the A domains if the system is an ABC or an ABB star copolymer system., Comment: 10 pages with 6 figures

Spatially localized exact solutions of plane Couette flow are organized in a snakes-and-ladders structure strikingly similar to that observed for simpler pattern-forming partial differential equations. [PRL 104,104501 (2010)]. We demonstrate the mechanism by which these snaking solutions originate from well-known periodic states of the Taylor-Couette system. They are formed by a localized slug of Wavy-Vortex flow that emerges from a background of Taylor vortices via a modulational sideband instability. This mechanism suggests a deep connection between pattern-formation theory and Navier-Stokes flow., Comment: 5 pages

Intensive care clinicians are presented with large quantities of patient information and measurements from a multitude of monitoring systems. The limited ability of humans to process such complex information hinders physicians to readily recognize and act on early signs of patient deterioration. We used machine learning to develop an early warning system for circulatory failure based on a high-resolution ICU database with 240 patient years of data. This automatic system predicts 90.0% of circulatory failure events (prevalence 3.1%), with 81.8% identified more than two hours in advance, resulting in an area under the receiver operating characteristic curve of 94.0% and area under the precision-recall curve of 63.0%. The model was externally validated in a large independent patient cohort., Comment: 5 main figures, 1 main table, 13 supplementary figures, 5 supplementary tables; 250ppi images