151. Scopulariopsis, a poorly known opportunistic fungus: spectrum of species in clinical samples and in vitro responses to antifungal drugs.
- Author
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Sandoval-Denis M, Sutton DA, Fothergill AW, Cano-Lira J, Gené J, Decock CA, de Hoog GS, and Guarro J
- Subjects
- Cluster Analysis, DNA, Fungal chemistry, DNA, Fungal genetics, DNA, Ribosomal chemistry, DNA, Ribosomal genetics, Genes, rRNA, Humans, Microbial Sensitivity Tests, Molecular Sequence Data, Mycological Typing Techniques, Mycoses epidemiology, Peptide Elongation Factor 1 genetics, Phylogeny, RNA, Fungal genetics, RNA, Ribosomal genetics, Scopulariopsis classification, Sequence Analysis, DNA, Antifungal Agents pharmacology, Mycoses microbiology, Scopulariopsis drug effects, Scopulariopsis isolation & purification
- Abstract
Ninety-nine isolates of clinical origin, tentatively identified as Scopulariopsis or Microascus, were morphologically and molecularly characterized by a combined analysis of the D1/D2 domains of the 28S rRNA gene and a fragment of the elongation factor 1-α gene (EF1-α) sequences. The most prevalent species was Scopulariopsis brevicaulis (49.4%), followed by Scopulariopsis gracilis (14.4%), Scopulariopsis brumptii (7.2%), Microascus cinereus (5.2%), the Scopulariopsis candida species complex (3.1%), and Microascus cirrosus (2.1%). The most common anatomic sites of isolation were the respiratory tract (61.6%), superficial tissue (19.2%), and deep tissue or fluid samples (19.2%). The antifungal susceptibilities of the isolates to eight drugs were tested in vitro, with all the drugs generally showing poor activity.
- Published
- 2013
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