151. Overexpression of the gene encoding HMG-CoA reductase in Saccharomyces cerevisiae for production of prenyl alcohols.
- Author
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Ohto C, Muramatsu M, Obata S, Sakuradani E, and Shimizu S
- Subjects
- Bioreactors, Fatty Alcohols chemistry, Fungal Proteins biosynthesis, Fungal Proteins genetics, Hydroxymethylglutaryl CoA Reductases chemistry, Industrial Microbiology methods, Metabolic Networks and Pathways genetics, Mevalonic Acid metabolism, Promoter Regions, Genetic, Protein Structure, Tertiary, Recombinant Proteins biosynthesis, Recombinant Proteins genetics, Terpenes metabolism, Fatty Alcohols metabolism, Hydroxymethylglutaryl CoA Reductases biosynthesis, Hydroxymethylglutaryl CoA Reductases genetics, Saccharomyces cerevisiae enzymology
- Abstract
To develop microbial production method for prenyl alcohols (e.g., (E,E)-farnesol (FOH), (E)-nerolidol (NOH), and (E,E,E)-geranylgeraniol (GGOH)), the genes encoding enzymes in the mevalonate and prenyl diphosphate pathways were overexpressed in Saccharomyces cerevisiae, and the resultant transformants were evaluated as to the production of these alcohols. Overexpression of the gene encoding hydroxymethylglutaryl (HMG)-CoA reductase was most effective among the genes tested. A derivative of S. cerevisiae ATCC 200589, which was selected through screening, was found to be the most suitable host for the production. On cultivation of the resultant transformant, in which the HMG-CoA reductase gene was overexpressed, in a 5-liter bench-scale jar fermenter for 7 d, the production of FOH, NOH, and GGOH reached 145.7, 98.8, and 2.46 mg/l, respectively.
- Published
- 2009
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