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OBJECTIVE: To Study Role of Autologous Platelet Rich Plasma (APRP) in Wound Healing. METHODS: This is a retrospective analysis of 19 patients with 21 chronic non healing wounds of different etiologies where Autologous Platelet Rich Plasma (APRP) was used. Patients unfit for anesthesia or refusing for surgery were managed only with APRP (Group-1) and in remaining patients who were fit for anesthesia and willing for surgery, wound bed was prepared by APRP and then covered with Skin Graft or Flap (Group-2). RESULTS: In Group-1 all wounds (10 patients, 11 wounds) healed by APRP. In Group-2 (9 patients, 10 wounds), 6 wounds healed by APRP with Skin Graft and 4 wounds healed by APRP with flap cover. CONCLUSION: APRP helps in healing of chronic non healing wounds. Those patients who are not fit for anesthesia or refusing for surgery can be managed with APRP only. Patients who are fit for anesthesia and ready for surgery, APRP accelerates wound bed preparation for cover by graft or flap. [ABSTRACT FROM AUTHOR]

Introduction: Basal cell carcinoma (BCC) is a locally invasive, slowly spreading tumor arising in the basal layer of epidermis and rarely metastasizes. Surgical excision with adequate margins is curative. Reconstruction of post-excisional defects on the face is both essential and challenging. Clinical Cases and Methods: A retrospective review of hospital records for patients operated for BCC of the face excluding the pinna at our institute in the last 3 years was done and a review of the literature was carried out to identify the most common principles governing the optimal reconstruction of post-excisional defects on the face. Literature search was made in Embase, Medline, and Cochrane databases in the last two decades with the filters placed for human and English language studies with the search terms (Facial Basal cell carcinoma) AND reconstruction AND (Humans[Mesh]). Results: Records of 32 patients with facial BCC who underwent excision and reconstruction at our hospital were identified and details were recorded. Our literature search with the terms and filters mentioned above revealed 244 studies with duplicates removed. After further hand-searching, 218 journal articles were identified, reviewed, and a reconstruction algorithm was designed based on the findings. Discussion: Reconstruction of post-BCC excisional defects of the face relies on an adequate understanding of the general principles of reconstruction, subunit principle of facial esthetics, flap anatomy and vascularity as well as operator experience. Complex defects need innovative solutions, multidisciplinary approaches, and newer methods of reconstruction like perforator flaps and newer techniques like supermicrosurgery. Conclusion: Multiple reconstructive options for post-excisional defects of the BCC over the face are available and most defects can be approached in an algorithmic manner. Further well-designed prospective research studies are needed to compare outcomes of different reconstructive options for a given defect and identify the most suitable options. [ABSTRACT FROM AUTHOR]

Lead-free, high piezocoefficient 0.5Ba(Zr0.2Ti0.8)O3–0.5(Ba0.7Ca0.3)TiO3 film is fabricated on soft magnetic Ni foil as a substrate using a pulse laser deposition technique for the magnetoelectric studies. The x-ray diffraction analysis confirms the phase pure formation of polycrystalline ferroelectric film along with the presence of NiO formed during the high temperature growth process. The obtained lattice parameters suggest that the native oxide could favour the coherent growth of a ferroelectric layer. The film exhibits ferroelectric characteristics with a polarization value of 23 µC cm−2 at 225 kV cm−1. The fabricated heterostructure displayed a high magnetoelectric coupling coefficient with the maximum magnetoelectric voltage coefficient (α31) value of 1.08 V cm−1 Oe−1 at low field of 44 Oe. It is noteworthy to mention that the observed α31 is comparable to the earlier reported toxic lead-based similar systems. The comparison between the experimental and calculated α31 values suggests that the strain transfer percentage efficiency is around 69%, which is responsible for the observed large ME coupling. These experimental findings give a way to achieve high magnetoelectric coefficients by fabricating the oxide piezoelectric layer directly on the clamping free magnetic substrate. [ABSTRACT FROM AUTHOR]

The present study dealt with the five marine microalgae strains viz., Chloroidium saccharophilum, Picochlorum maculatum, Amphora sp., Hymenomonas globosa and Limnospira indica and their effective degradation ability of Low-Density Polyethylene for the period of 45 days. The incubation of LDPE in microalgae culture has resulted in the maximum weight loss (20.16 ± 0.14 %), higher reduction rate (0.005/day) and lower half-life (138.4 days) in the LDPE treated with P. maculatum. The SEM images of all treated LDPE revealed surface erosion and the ATR-FTIR spectra showed functional group peaks along with new peaks at 1369.35 cm -1 , 2332.96 cm -1 and 500-726 cm -1 . Carbonyl (Keto, Ester), Vinyl and Internal double bond indices increased significantly in all the treated groups. The crystallinity was decreased (64.13 %) in P. maculatum treated LDPE than the control (71.37 %). Thermogravimetric analysis showed the reduction in thermal stability after biodegradation. This efficient microalgal degradation as a bioremediation technique will reduce the plastic pollution., Competing Interests: Declaration of competing interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper., (Copyright © 2023 Elsevier Ltd. All rights reserved.)

Poor proteolytic resistance, toxicity and salt/serum sensitivity of antimicrobial peptides (AMPs) limits their practical clinical application. Here, to overcome these drawbacks of AMPs and develop novel antimicrobial agents, a series of small molecules based on a triazine-piperazine-triazine scaffold that mimic the cationic amphipathic structure of AMPs were synthesized and evaluated their potential as a new class of antimicrobial agents. All designed compounds showed strong antimicrobial activity and negligible hemolytic activity. Particularly, five compounds (9, 11, 12, 15, and 16) presented excellent cell selectivity with proteolytic resistance, salt/serum stability and anti-inflammatory activity against lipopolysaccharide (LPS)-induced inflammation. These five compounds exhibited similar or 2-4 fold higher antimicrobial activity than melittin against six antibiotic-resistant bacteria tested. Similar to the intracellular-targeting AMP, buforin-2, these compounds displayed an intracellular mode of antimicrobial action. These compounds showed potent biofilm inhibitory and eradicating activities against multidrug-resistant Pseudomonas aeruginosa (MDRPA). Additionally, these compounds displayed synergistic or additive effects when combined with selected clinically used antibiotics. Furthermore, these compounds have been proven to inhibit pro-inflammatory cytokine release by directly binding to LPS and blocking the interaction between LPS and CD14/TLR4 receptor in LPS-stimulated RAW264.7 macrophage cells. Overall, our results demonstrate the potential of the designed compounds as a novel class of multifunctional antimicrobial agents to combat bacterial infection., Competing Interests: Declaration of competing interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper., (Copyright © 2022 Elsevier Masson SAS. All rights reserved.)

Occupancy of waste micro plastic particles in the beach sand is found hazardous sea livings as well as creates the environmental issues. Many research attempts have been made to short out them. This investigation focuses on utilizing such micro plastic to produce cost effective ABS plasticproducts including toys manufacturing. The screened out micro plastic particles were chemically refined to obtain the pro form of Polypropylene (PP) and Polystyrene (PS) and then they used (10 wt%, 15 wt%, 20 wt%and 25 wt%) with raw ABS plastic and prepared billets by injection moulding. The moulding parameters like melting temperature (230 °C, 240 °C, 250 °C and 260 °C), injection pressure (1300 bar, 1400 bar, 1500 bar and 1600 bar) and injection time (0.4s, 0.8s, 1.2s and 1.6s) to maximize the Impact, tensile and flexural strengths of proposed plastic with Taguchi method. The results reveal that 25% micro plastic reinforced specimen prepared at 250 °C, 1400 bar and 1.6s, is outperformed., (Copyright © 2022 Elsevier Ltd. All rights reserved.)

Most of the 'oil refineries' severally pollutes the water resources by depleting their untreated waste water like cooling water, storm water and unsanitary sewage water. These wastewaters are to be treated with high care to protect the human, pebbles, plants, fish and other water animals and from harmful effects. The present study focused to treat the oil refinery wastewater by means of Multi wall carbon nanotube (MWCNT) coated Polyvinylidene Fluoride (PVDF) membrane. The main objectives are: to increases the life of filter, reduce the percolation flux and reduce the formation of antifouling in the filter by using MWCNT composite membrane in it. Different process parameters of the proposed water treatment process, like diameter of MWCNT (15 nm, 20 nm, 25 nm and 30 nm), operating pressure (3 bar, 4 bar, 5 bar and 6 bar), pH value (3, 5, 7 and 9) and temperature (25 °C, 30 °C, 35 °C and 40 °C) temperature. Taguchi statistical technique is employed for designing experiments and for optimizing the process parameters of wastewater treatment process of an oil refinery. The proposed filter for wastewater treatment exhibited appreciable performance in removal rate of Percolation flux, percentage of chemical oxygen demand removal and percentage of total carbolic rejection as 27.2 kg/m 2 h, 78.51% and 95.33% respectively., (Copyright © 2022 Elsevier Ltd. All rights reserved.)

The present study aimed to evaluate the microplastic degradation efficiency of bacterial isolates collected from Vaigai River, Madurai, India. The isolates were processed with proper methods and incorporated in to the UV-treated polyethylene (PE) and polypropylene (PP) degradation. Based on preliminary screening, four bacterial isolates such as Bacillus sp. (BS-1), Bacillus cereus (BC), Bacillus sp. (BS-2), and Bacillus paramycoides (BP) were proceed to further degradation experiment for 21 days. The microplastics were filled with bacterial isolates which is use microplastic (PE, PP) as carbon source for their growth and proceed for shake flask experiment were carried out by two approaches with control. The microplastic degradation was confirmed through their weight loss, increasing fragmentations and changes of surface area against control experiments (microplastic without isolates) also confirms degrading efficiency of isolated bacterial strains through non-changes in their weight and surface area. The highest degradation of PP and PE were observed in BP (78.99 ± 0.005%), and BC (63.08 ± 0.009%) in single approach, while in combined approach BC & BP recorded the highest degradation in both PP (78.62 ± 2.16%), and PE (72.50 ± 20.53%). The formation of new functional groups is confirming the biofilm formation in the surface area of microplastics by isolates and proving their efficiency in degrade the microplastics. The degradation of microplastic experiments should be cost effective and zero waste which is helpful to save the environment and the present findings could reveal the way to degrade the microplastics and prevent the microplastic pollution in aquatic environment., (© 2021. The Author(s), under exclusive licence to Springer-Verlag GmbH Germany, part of Springer Nature.)

The present study was aimed to assess the effect of gibberellic acids to enhance the growth, biomass, pigment, and exopolysaccharides production in Tetraselmis suecica under reciprocal nitrogen concentrations. For this study, the seven types of experimental media (N-P, NL-P/2GA3, N0-P/2GA3, NL-P/4GA3, N0-P/4GA3, NL-P/6GA3, and N0-P/6GA3) were prepared with the addition of gibberellic acids under various nitrogen concentrations. The experiment lasted for 15 days and the cell density, biomass, chlorophyll 'a', and exopolysaccharides (EPS) concentration of T. suecica were estimated for every 3 days. Then the EPS was subjected to the analyses of chemical (carbohydrate, protein, sulfate, and uronic acid), and antioxidant activity. In addition, nutrient removal efficiency was evaluated using different concentration of EPS. The highest DPPH (2,2-diphenyl-1-picrylhydrazyl) (86.7 ± 0.95%) and hydroxyl radical activity (85.7 ± 2.48%) were observed at the EPS concentrations 2.5 and 1.2 mg/mL, respectively. The immobilized magnetic Fe 3 O 4 -EPS (ferric oxide-exopolysaccharides) nanoparticles (5.0 and 10.0 g/L) have efficiently removed the excessive phosphate (89.5 ± 1.65%) and nitrate (73.5 ± 1.72%) from the Litopenaeus vannamei cultured wastewater. Thus, the application of gibberellic acids combined with limited nitrogen concentration could produce higher EPS that could exhibit excellent antioxidant activity, and nutrient removal efficacy in the form of Fe 3 O 4 -EPS magnetic nanoparticles., (© 2021. The Author(s), under exclusive licence to Springer-Verlag GmbH Germany, part of Springer Nature.)

Electron correlation in transition metal oxides (TMOs) is an intriguing topic in condensed matter physics, revealing a wide variety of exotic physical properties. Investigating low-energy carrier dynamics by terahertz (THz) spectroscopy is an efficient route to obtain the essential insights into electron correlation. In the present study, THz-time-domain spectroscopy is employed to probe electron correlation in SrVO 3 epitaxial thin films. The low energy carrier dynamics of SrVO 3 in the range of 0.2-6.0 meV shows a typical metallic behavior as overserved in dc transport measurements. The obtained temperature-dependent optical parameters provide evidence of mass renormalization in the low energy regime and carrier momentum relaxation happens via the electron-electron scattering mechanism. Overall, the frequency and temperature-dependent optical parameters indicate the Fermi liquid ground state in a Mott-Hubbard type correlated metal SrVO 3 thin film. Our results provide significant insight on low energy carrier dynamics in the correlated electron system, particularly perovskite-based d 1 TMOs., (© 2021 IOP Publishing Ltd.)

Background: Occurrence of multiple biotic stresses on crop plants result in drastic yield losses which may have severe impact on the food security. It is a challenge to design strategies for simultaneous management of these multiple stresses. Hence, establishment of innovative approaches that aid in their management is critical. Here, we have introgressed a micro RNA-induced gene silencing (MIGS) based combinatorial gene construct containing seven target gene sequences of cotton leaf curl disease (CLCuD), cotton leaf hopper (Amrasca biguttula biguttula), cotton whitefly (Bemisia tabaci) and root-knot nematode (Meloidogyne incognita)., Results: Stable transgenic lines of Nicotiana benthamiana were generated with the T-DNA harboring Arabidopsis miR173 target site fused to fragments of Sec23 and ecdysone receptor (EcR) genes of cotton leaf hopper and cotton whitefly. It also contained C2/replication associated protein (C2/Rep) and C4 (movement protein) along with βC1 gene of betasatellite to target CLCuD, and two FMRFamide-like peptide (FLP) genes, Mi-flp14 and Mi-flp18 of M. incognita. These transgenic plants were assessed for the amenability of MIGS approach for pest control by efficacy evaluation against M. incognita. Results showed successful production of small interfering RNA (siRNA) through the tasiRNA (trans-acting siRNA) pathway in the transgenic plants corresponding to Mi-flp18 gene. Furthermore, we observed reduced Mi-flp14 and Mi-flp18 transcripts (up to 2.37 ± 0.12-fold) in females extracted from transgenic plants. The average number of galls, total endoparasites, egg masses and number of eggs per egg mass reduced were in the range 27-62%, 39-70%, 38-65% and 34-49%, respectively. More importantly, MIGS transgenic plants showed 80% reduction in the nematode multiplication factor (MF)., Conclusion: This study demonstrates successful validation of the MIGS approach in the model plant, N. benthamiana for efficacy against M. incognita, as a prelude to translation to cotton. © 2021 Society of Chemical Industry., (© 2021 Society of Chemical Industry.)

The present study aimed to investigate the water quality characteristics and the ecological indicators of wastewater of white legged shrimp Penaeus vannamei ponds along the Southeast coast of India. The wastewater samples were collected from 15 shrimp farms covering 11 districts located along the coastal line of Tamil Nadu and Puducherry, India. By adopting standard methods, the collected samples were subjected to analyses of physico-chemical and biological characteristics, especially the microbial load and metal and plankton composition. The nitrate-nitrogen, ammonia-nitrogen, THB, TCB, and Cu concentrations of the samples were found to exceed the permissible limit as recommended by WHO, USEPA, CPCB, and CAA. Principal component analysis and canonical correspondence analysis have suggested that the phosphate, nitrate, silicate, ammonia, and total phosphorus are the important chemical factors. The generated data would be of interest to farmers for their shrimp crop management vis-à-vis culture pond wastewater treatment., (Copyright © 2020 Elsevier Ltd. All rights reserved.)

The bioaccumulation of heavy metals (Zn, Pb, Cd, Co, Cu, and Fe) in fish tissues was determined using Atomic Absorption Spectrometer (spectra 220) and Mercury (Hg) content was estimated by using a mercury analyzer. Various organs (eye, gill, gut, gonad, liver, skin, and muscle) of three fish species, i.e., Labeo rohita, Pangasius hypophthalmus and Katsuwonus pelamis, from Visakhapatnam were analyzed. Young and aged fish were chosen based on their length: LR-32, LR-49 (Rohu); PH-33, PH-56 (Pangasius); and KP-18, KP-52 (Skipjack). In both the small and big size of L. rohita and P. hypophthalmus, the metal concentration was in the order Fe > Zn > Cu whereas Pb, Hg, and Co were below detectable limit (BDL). Concentrations in K. pelamis was Fe > Zn > Cu > Cd and Pb, Hg, and Co were BDL. In conclusion, the metal concentrations in the examined fish species available in Visakhapatnam fall below the maximum permissible limit (MPL) for human consumption recommended by FAO, WHO, MAFF, and EC., Competing Interests: Declaration of competing interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper., (Copyright © 2020 Elsevier Ltd. All rights reserved.)

An in planta gene editing approach was developed wherein Cas9 transgenic plants are infected with an RNA virus that expresses single guide RNAs (sgRNAs). The sgRNAs are augmented with sequences that promote cell-to-cell mobility. Mutant progeny are recovered in the next generation at frequencies ranging from 65 to 100%; up to 30% of progeny derived from plants infected with a virus expressing three sgRNAs have mutations in all three targeted loci.

Drug delivery using synthetic mesoporous nanomaterials, including porous silicon, has been extensively used to ameliorate the constraints currently experienced with conventional chemotherapy. Owing to the amazing potential, the silica based nanomaterials have been used widely. Nevertheless, synthetic nanomaterial involves high cost, lack of scalability, and the use of toxic substances limits its utilization. These issues can be overcome by the use of nature generated nanoscale materials, such as diatoms would serve as a boon for pharmaceutical industries. In this study we investigate the use of a mesoporous, biodegradable nanomaterial obtained from the natural silica found in the diatom species Amphora subtropica (AMPS) for drug delivery applications. AMPS cultures cleaned and chemically treated to obtain Amphora frustules (exoskeleton) (AF), followed by surface functionalization with chitosan (Chi). Results of our experiments demonstrate high drug loading, strong luminescence, biodegradable and biocompatible nature of the doxorubicin tethered diatom. Further, toxicity studies employing immortalized lung cancer cell line (A549) indicates sustained drug delivery and less toxic compared to the free doxorubicin (DOX), suggesting AF could be an excellent substitute for synthetic nanomaterials used in drug delivery applications., (Copyright © 2018 Elsevier B.V. All rights reserved.)

CXCL14 is a CXC chemokine family that exhibits antimicrobial activity and contains an amphipathic cationic α-helical region in the C-terminus, a characteristic structure of antimicrobial peptides (AMPs). In this study, we designed three analogs of CXCL14 59-75 (named CXCL14-C17) corresponding to the C-terminal α-helix of CXCL14, which displayed potential antimicrobial activity against a wide variety of gram-negative and gram-positive bacteria with minimum inhibitory concentrations of 4-16 μM without mammalian cell toxicity. Furthermore, two CXCL14-C17 analogs (CXCL14-C17-a1 and CXCL14-C17-a3) with improved cell selectivity were engineered by introducing Lys, Arg, or Trp in CXCL14-C17. Additionally, CXCL14-C17 analogs showed much greater synergistic effect (FICI: 0.3125-0.375) with chloramphenicol and ciprofloxacin against multidrug-resistant Pseudomonas aeruginosa (MDRPA) than LL-37 did (FICI: 0.75-1.125). CXCL14-C17 analogs were more active against antibiotic-resistant bacteria including methicillin-resistant Staphylococcus aureus (MRSA), MDRPA, and vancomycin-resistant Enterococcus faecium (VREF) than LL-37 and melittin. In particular, CXCL14-C17-a2 and CXCL14-C17-a3 completely inhibited the biofilm formation at sub-MIC and all of the peptides were able to eliminate pre-formed biofilm as well. Membrane depolarization, flow cytometry, sytox green uptake, ONPG hydrolysis and confocal microscopy revealed the possible target of the native peptide (CXCL14-C17) to likely be intracellular, and the amphipathic designed analogs targeted the bacterial membrane. CXCL14-C17 also showed DNA binding characteristic activity similar to buforin-2. Interestingly, CXCL14-C17-a2 and CXCL14-C17-a3 effectively inhibited the production and expression of nitric oxide (NO), tumor necrosis factor (TNF)-α, interleukin (IL)-6, and monocyte chemoattractant protein (MCP)-1 from lipopolysaccharide (LPS)-stimulated RAW264.7 cells, suggesting that these peptides could be promising anti-inflammatory and antimicrobial agents., (Copyright © 2018 Elsevier B.V. All rights reserved.)

The improvisation of the treatment procedures for treating the various kind of bone defects such as, bone or dental trauma and for diseases such as osteoporosis, osteomyelitis etc., need the suitable and promising biomaterials with resemblance of bone components. Bioactive glass ceramic (BGC) has recently acquired great attention as the most promising biomaterials; hence it has been widely applied as a filler material for bone tissue regeneration. Because it elicts specific biological responses after implantation in addition more potential in formation of strong interface with both hard and soft tissues by dissolution of calcium and phosphate ions. Hence, the current focus in treating the bone defects by orchestrating the biomaterial in combination of alternative medicine such as homeopathic remedies with biomaterials to prevent the adverse effects at minimal concentrations. So the current study was focused on constructing the nano-bioglass ceramic particles (nBGC) doped with novel homeopathic remedy Calcarea phosphorica for dental and bone therapeutic implants. The nBGC particles were synthesized by sol-gel method and reinforced with commercially available Calcarea phosphorica. The synthesized particles were characterized by SEM, DLS, EDS, FT-IR, and XRD studies. The SEM and DLS were shown the size of the particles at nano scale, also the EDS, and FT-IR investigations indicated that the Calcarea phosphorica was integrated with nBGC particles and also the crystalline nature of particles was confirmed by XRD studies. Both nBGC and Calcarea phosphorica doped nBGC (CP-nBGC) were found to be non toxic to mouse mesenchymal stem cells at lower concentrations and also illustrated the better bone forming ability in vitro., (Copyright © 2017 Elsevier B.V. All rights reserved.)

The exquisite harmony between hormones and their corresponding signaling pathways is central to prioritizing plant responses to simultaneous and/or successive environmental trepidations. The crosstalk between jasmonic acid (JA) and salicylic acid (SA) is an established effective mechanism that optimizes and tailors plant adaptive responses. However, the underlying regulatory modules of this crosstalk are largely unknown. Global transcriptomic analyses of mutant plants (ceh1) with elevated levels of the stress-induced plastidial retrograde signaling metabolite 2-C-methyl-D-erythritol cyclopyrophosphate (MEcPP) revealed robustly induced JA marker genes, expected to be suppressed by the presence of constitutively high SA levels in the mutant background. Analyses of a range of genotypes with varying SA and MEcPP levels established the selective role of MEcPP-mediated signal(s) in induction of JA-responsive genes in the presence of elevated SA. Metabolic profiling revealed the presence of high levels of the JA precursor 12-oxo-phytodienoic acid (OPDA), but near wild type levels of JA in the ceh1 mutant plants. Analyses of coronatine-insensitive 1 (coi1)/ceh1 double mutant plants confirmed that the MEcPP-mediated induction is JA receptor COI1 dependent, potentially through elevated OPDA. These findings identify MEcPP as a previously unrecognized central regulatory module that induces JA-responsive genes in the presence of high SA, thereby staging a multifaceted plant response within the environmental context., (© The Author 2016. Published by Oxford University Press on behalf of the Society for Experimental Biology.)

The present study was investigated for soil bioremediation through sababul plant biomass (Leucaena leucocephala). The soil contaminated with textile effluent was collected from Erode (chithode) area. Various physico-chemical characterizations like N, P, and K and electrical conductivity were assessed on both control and dye contaminated soils before and after remediation. Sababul (L. leucocephala) powder used as plant biomass for remediation was a tool for textile dye removal using basic synthetic dyes by column packing and eluting. The concentration of the dye eluted was compared with its original concentration of dye and were analyzed by using UV-vis spectrophotometer. Sababul plant biomass was analyzed for its physico-chemical properties and active compounds were detected by GC-MS, HPTLC and FTIR. Plant growth was assessed with green gram on the textile contaminated soil and sababul had the potential of adsorbing the dye as the contaminated soil and also check the growth of green gram.

Oral cancer is a major public health problem in India. Most patients present with locally advanced disease requiring complex resection and reconstruction strategies. Costs, operating time and availability of expertise are major issues that influence efficient health delivery, especially in developing countries such as India. Technically simple and widely reproducible techniques may be used successfully where applicable, to overcome these issues. The submental artery flap is a well described and acceptable alternative to the radial artery forearm free flap in oral cavity reconstruction. Researchers have demonstrated its technical ease of performance and reproducibility amongst trainees. Here the authors describe the bipaddled submental artery flap, a modification of the standard flap, which can be used to provide lining as well as skin cover for a full thickness cheek defect. Two skin paddles are fashioned taking advantage of the vascular anatomy of the submental vessels., (Copyright © 2011 International Association of Oral and Maxillofacial Surgeons. Published by Elsevier Ltd. All rights reserved.)

Receptors localized at the plasma membrane are critical for the recognition of pathogens. The molecular determinants that regulate receptor transport to the plasma membrane are poorly understood. In a screen for proteins that interact with the FLAGELIN-SENSITIVE2 (FLS2) receptor using Arabidopsis thaliana protein microarrays, we identified the reticulon-like protein RTNLB1. We showed that FLS2 interacts in vivo with both RTNLB1 and its homolog RTNLB2 and that a Ser-rich region in the N-terminal tail of RTNLB1 is critical for the interaction with FLS2. Transgenic plants that lack RTNLB1 and RTNLB2 (rtnlb1 rtnlb2) or overexpress RTNLB1 (RTNLB1ox) exhibit reduced activation of FLS2-dependent signaling and increased susceptibility to pathogens. In both rtnlb1 rtnlb2 and RTNLB1ox, FLS2 accumulation at the plasma membrane was significantly affected compared with the wild type. Transient overexpression of RTNLB1 led to FLS2 retention in the endoplasmic reticulum (ER) and affected FLS2 glycosylation but not FLS2 stability. Removal of the critical N-terminal Ser-rich region or either of the two Tyr-dependent sorting motifs from RTNLB1 causes partial reversion of the negative effects of excess RTNLB1 on FLS2 transport out of the ER and accumulation at the membrane. The results are consistent with a model whereby RTNLB1 and RTNLB2 regulate the transport of newly synthesized FLS2 to the plasma membrane.

Research in autophagy continues to accelerate,(1) and as a result many new scientists are entering the field. Accordingly, it is important to establish a standard set of criteria for monitoring macroautophagy in different organisms. Recent reviews have described the range of assays that have been used for this purpose.(2,3) There are many useful and convenient methods that can be used to monitor macroautophagy in yeast, but relatively few in other model systems, and there is much confusion regarding acceptable methods to measure macroautophagy in higher eukaryotes. A key point that needs to be emphasized is that there is a difference between measurements that monitor the numbers of autophagosomes versus those that measure flux through the autophagy pathway; thus, a block in macroautophagy that results in autophagosome accumulation needs to be differentiated from fully functional autophagy that includes delivery to, and degradation within, lysosomes (in most higher eukaryotes) or the vacuole (in plants and fungi). Here, we present a set of guidelines for the selection and interpretation of the methods that can be used by investigators who are attempting to examine macroautophagy and related processes, as well as by reviewers who need to provide realistic and reasonable critiques of papers that investigate these processes. This set of guidelines is not meant to be a formulaic set of rules, because the appropriate assays depend in part on the question being asked and the system being used. In addition, we emphasize that no individual assay is guaranteed to be the most appropriate one in every situation, and we strongly recommend the use of multiple assays to verify an autophagic response.

Protein microarray technology has emerged as a powerful new approach for the study of thousands of proteins simultaneously. Protein microarrays have been used for a wide variety of applications for the human and yeast systems. In a recent study, we demonstrated that Arabidopsis functional protein microarrays can be generated and employed to characterize the function of plant proteins. The arrayed proteins were produced using an optimized large-scale plant-based expression system. In a proof-of concept study, 173 known and novel potential substrates of calmodulin (CaM) and calmodulin-like proteins (CML) were identified in an unbiased and high-throughput manner. The information documented here on novel potential CaM targets provides new testable hypotheses in the area of CaM/Ca(2+)-regulated processes and represents a resource of functional information for the scientific community.