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Chlamydophila abortus and Waddlia chondrophila cause abortion in ruminants. We investigated the role of Parachlamydia acanthamoebae in bovine abortion. Results of immunohistochemical analyses were positive in 30 (70%) of 43 placentas from which Chlamydia-like DNA was amplified, which supports the role of Parachlamydia spp. in bovine abortion.

Aim: To determine the presence of Chlamydia pneumoniae in carotid plaque and peripheral blood mononuclear cells (PBMCs) using a combination of serology, direct antigen detection by immunofluorescence (IF) microscopy and polymerase chain reaction (PCR), and to compare the results obtained from each assay., Methods: A total of 54 atherosclerotic carotid plaques were tested for the presence of Chlamydia by PCR and IF methods. Of these 54 patients with carotid artery disease (CAD), 43 were also tested for the presence of C. pneumoniae DNA in PBMCs and for Chlamydia antibodies using two methods, the Medac Chlamydien rELISA and Focus Chlamydia microimmunofluorescence (MIF) methods., Results: Eighteen of the 54 (33%) carotid specimens were positive for the presence of C. pneumoniae DNA, whereas only two of 43 (5%) patients had C. pneumoniae DNA present within their PBMC fraction. Chlamydial antibodies were detected by MIF and/or rELISA in 56% (24/43) of the patients tested. None of the 43 patients was C. pneumoniae positive in all of the test specimens (plaque, PBMCs and serum)., Conclusions: Chlamydia pneumoniae is commonly found in Australian patients with CAD. Serology and PCR-based detection of C. pneumoniae in PBMCs and plaque give highly discordant results.

Porcine Chlamydiaceae were cultivated under various culture conditions and we compared their growth characteristics with those of ruminant and avian strains. The combination of centrifugation assisted cell culture infection and cycloheximide treatment of Vero cell coverslip cultures provided the highest inclusion numbers with all chlamydial strains. Interestingly, the use of Iscove's modified Dulbecco's medium instead of Eagle's minimal essential medium significantly increased Chlamydia suis inclusion counts. C. suis and Chlamydophila pecorum inclusion numbers were markedly increased in CaCo cells, compared with Vero cells. This accelerated growth of porcine Chlamydiaceae under certain cultivation conditions may be helpful for the propagation of low chlamydial numbers or for their isolation from field samples. The intracellular distribution of porcine Chlamydiaceae in polarised CaCo cells clearly demonstrated differences between the chlamydial strains: C. pecorum 1710S inclusions were predominantly localised in the apical cytoplasm, C. suis S45 inclusions, however, were mostly situated in lower cytoplasmatic compartments. These findings might reflect biological differences in vivo.