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Introduction: Lifestyle factors, as eating habits and physical activity, are associated with health and productivity of workers. The aim of this study is an assessment of lifestyle of the employees of an international company of household items., Methods: 291 (170 female, 121 male) employees underwent body composition assessment and completed two questionnaires (International Physical Activity Questionnaire and National Institute for Research of the Food and Nutrition)., Results: The weight status of the sample is at the upper limits of normal weight. Products based on cereals are consumed about once a day, fresh meat 3.0 (0.0-12.0) per week, fresh fruit (5.0, 0.0-25.0) and vegetables (6.0, 0.0-14.0) less than one serving a day. No vigorous physical activity is performed (0.0, 0.0-240.0 min/week), moderate is performed 30.0 (0.0-450.0) min/week and only 106 subjects were aware of the number of daily steps., Conclusions: Job duties can have an influence on the daily habits. Workplaces have great potential to change personal lifestyle choices and a preliminary assessment should be performed in order to propose a tailored intervention.

Salicylic acid (SA) plays a central role in plant responses to environmental stresses. In a recent study, we suggested a third pathway for SA biosynthesis from mandelonitrile (MD) in peach plants. This pathway is an alternative to the phenylalanine ammonia-lyase pathway and links SA biosynthesis and cyanogenesis. In the present work, using biochemical approaches, we studied the effect of salt stress and Plum pox virus (PPV) infection on this proposed SA biosynthetic pathway from MD. Peach plants were submitted to salt stress and Plum pox virus (PPV) infection. We studied the levels of SA and its intermediates/precursors (phenylalanine, MD, amygdalin and benzoic acid) in in vitro shoots. Moreover, in peach seedlings, we analysed the content of H 2 O 2 -related enzymes, SA and the stress-related hormones abscisic acid and jasmonic acid. We showed that the contribution of this SA biosynthetic pathway from MD to the total SA pool does not seem to be important under the stress conditions assayed. Nevertheless, MD treatment not only affected the SA content, but also had a pleiotropic effect on abscisic acid and jasmonic acid levels. Furthermore, MD modulates the antioxidative metabolism via SA-dependent or -independent redox-related signalling pathways. Even though the proposed SA biosynthetic pathway seems to be functional under stress conditions, MD, and hence cyanogenic glycosides, may be operating more broadly than by influencing SA pathways and signalling. Thus, the physiological function of the proposed SA biosynthetic pathway remains to be elucidated., (© 2019 German Society for Plant Sciences and The Royal Botanical Society of the Netherlands.)

A novel approach to local functionalization of plasmonic hotspots at gold nanoparticles with biofunctional moieties is reported. It relies on photocrosslinking and attachment of a responsive hydrogel binding matrix by the use of a UV interference field. A thermoresponsive poly( N -isopropylacrylamide)-based (pNIPAAm) hydrogel with photocrosslinkable benzophenone groups and carboxylic groups for its postmodification was employed. UV-laser interference lithography with a phase mask configuration allowed for the generation of a high-contrast interference field that was used for the recording of periodic arrays of pNIPAAm-based hydrogel features with the size as small as 170 nm. These hydrogel arrays were overlaid and attached on the top of periodic arrays of gold nanoparticles, exhibiting a diameter of 130 nm and employed as a three-dimensional binding matrix in a plasmonic biosensor. Such a hybrid material was postmodified with ligand biomolecules and utilized for plasmon-enhanced fluorescence readout of an immunoassay. Additional enhancement of the fluorescence sensor signal by the collapse of the responsive hydrogel binding matrix that compacts the target analyte at the plasmonic hotspot is demonstrated., Competing Interests: The authors declare no competing financial interest., (Copyright © 2020 American Chemical Society.)

Soccer referees are a specific group of the athletes' population whose careers peak from 30 to 45 years old. An athlete's performance is not only determined by physical training but also by a lifestyle, e.g. eating habits. The purpose of this study was to verify current eating habits and resulting body composition of a group of elite international soccer referees. At an international FIFA seminar 60 elite international soccer referees (aged 39.2 ± 4.2 years) were enrolled. A body composition assessment was performed with skinfold thickness and bio impedance analysis, while eating habits were evaluated with a multi-pass 24-hour dietary recall. The body composition showed a normal weight condition with a fat content of 11.4 ± 2.5%. Macronutrients showed a low level of carbohydrates (43.6 ± 5.4%) and a high level of fat (40.0 ± 4.5%). Micronutrients showed a low level of calcium, potassium, zinc, magnesium, iodine, vitamin B12 and vitamin B9. Even though their body composition was within the normal range, the current eating habits of elite international soccer referees did not appear to follow the nutrition guidelines. Therefore, it would be advisable to provide knowledge on nutrition for this particular category of sports subjects, an individualized nutritional plan would be advisable, in order to achieve and maintain better performance and appropriate body composition for their role., (© 2020 Gabriele Mascherini, Cristian Petri, Elena Ermini, Angelo Pizzi, Antonio Ventura, Giorgio Galanti, published by Sciendo.)

The gene GIGANTEA (GI) appeared early in land plants. It is a single copy gene in most plants and is found in two to three copies in Solanaceae. We analyzed the silencing of one GI copy, Petunia hybrida GI1 (PhGI1), by hairpin RNAs in Petunia in order to gain knowledge about its range of functions. Decreased transcript levels of PhGI1 were accompanied also by a reduction of PhGI2. They were further associated with increased time period between two consecutive peaks for PhGI1 and CHANEL (PhCHL), the orthologue of the blue light receptor gene ZEITLUPE (ZTL), confirming its role in maintaining circadian rhythmicity. Silenced plants were bigger with modified internode length and increased leaf size while flowering time was not altered. We uncovered a new function for PhGI1 as silenced plants showed reduction of flower bud number and the appearance of two flower buds in the bifurcation point, were normally one flower bud and the inflorescence meristem separate. Furthermore, one of the flower buds consistently showed premature flower abortion. Flowers that developed fully were significantly smaller as a result of decreased cell size. Even so the circadian pattern of volatile emission was unchanged in the silenced lines, flowers emitted 20% less volatiles on fresh weight basis over 24 hours and showed changes in the scent profile. Our results indicate a novel role of PhGI1 in the development of reproductive organs in Petunia. PhGI1 therefore represses growth in vegetative plant parts, maintains the typical cymose inflorescence structure, and inhibits premature flower abortion.

The aim of the study is to establish a simple and low-cost method that, associated with Body Mass Index (BMI), differentiates overweight conditions due to a prevalence of lean mass compared to an excess of fat mass during the evaluation of young athletes. 1046 young athletes (620 male, 426 female) aged between eight and 18 were enrolled. Body composition assessments were performed with anthropometry, circumferences, skinfold, and bioimpedance. Overweight was established with BMI, while overfat was established with the percentage of fat mass: 3.5% were underweight, 72.8% were normal weight, 20.1% were overweight, and 3.5% were obese according to BMI; according to the fat mass, 9.5% were under fat, 63.6% were normal fat, 16.2% were overfat, and 10.8% were obese. Differences in overfat prediction were found using BMI alone or with the addition of the triceps fold (area under the receiver operating characteristics curve (AUC) for BMI = 0.867 vs. AUC for BMI + TRICEPS = 0.955, p < 0.001). These results allowed the creation of a model factoring in age, sex, BMI, and triceps fold that could provide the probability that a young overweight athlete is also in an overfat condition. The calculated probability could reduce the risk of error in establishing the correct weight status of young athletes.

Based on the high financial and logistical costs associated with the assessment of body composition with dual-energy X-ray absorptiometry (DXA), this study determined which field method has the best correlation with DXA data, and developed an equation to estimate fat-free mass (FFM) using the field anthropometric data in international soccer players. A total of 17 international soccer players participated in this study. DXA values provided a criterion measure of FFM. Correlation coefficients, biases, limits of agreement, and differences were used as validity measures, and regression analyses were used to develop the prediction equation. All field methods used to obtain FFM data showed positive correlations (r from 0.90-0.96) with DXA. Only the equation developed by Deurenberg et al. [6] showed no differences from DXA with a low bias. The main strength of this study was providing a valid and accurate equation to estimate FFM specifically in international soccer players., Competing Interests: Authors declare that they have no conflict of interest., (© Georg Thieme Verlag KG Stuttgart · New York.)

Background: Aerobic and resistance exercises have been promoted recently to improve quality of life in cancer patients. Most cancer survivorship rehabilitation programs consist of supervised exercise programs; however, less data is available on the effects of unsupervised or home-based exercise interventions. The study aimed to compare the physical and physiologic changes in a group of cancer survivors (CS) and a control group of non-cancer, health controls (HC) who participated in individualized home-based aerobic and resistance exercises for 12 months., Methods: Thirty-three surviving cancer survivors (CS) aged 55.6±3 years were enrolled for 1 year of unsupervised exercise prescription programs. Anthropometric parameters hydration status, fitness, and echocardiographic examination were measured every six month and compared to10 HC (aged 52.6±7.7 years) individuals prior to starting the program (t0) and at 6 (t6) and 12 (t12) months., Results: Among the CS subjects, a significant reductions in waist circumference (t0: 97.5±15.2 cm, t6: 86.6±13.5 cm, t12: 85.8±13.9 cm; P<0.05), body cell mass (t0: 50.9±4.7%, t6: 52.3±4.4%, t12: 53.7±3.7%; P<0.05), and extracellular mass (t0: 49.1±4.7%, t6: 47.6±4.4%, t12: 46.2±3.7%; P<0.05) were observed, as well significant improvements in lower body muscle strength (chair test t0: 13.3±4.1, t6: 14.2±3.5, t12: 15.1±3.2; P<0.05). Changes in functionality and heart function were similar between CS and HC., Conclusions: Individually-prescribed home-based exercise programs were cost effective, safe and resulted in modest improvements in body composition, strength, and total body water distribution with little to no adverse effect on cardiac function.

Background: Cardiac adaptation to intense physical training is determined by many factors. Differences between the sexes in the left ventricle parameters are well established, however, both sport activity and body composition seem to reduce them. In order to better explain some heart modifications, a first approach was to eliminate the FM from the indexing of the left ventricular parameters. Fat free mass also contains the extracellular mass which does not represent a metabolically active compartment. The aim of this study is to verify the differences between the sexes with a new left ventricular indexation with the metabolically active tissue of the body as body cell mass in elite athletes., Methods: Twenty-five females were matched with 25 elite male soccer players (females=26.2±1.9 yrs, males=26.5±1.8 yrs; P=NS). An accurate body composition analysis (skinfold and bioimpedance) and an echocardiography were performed., Results: Differences between the sexes in body composition were confirmed in fat mass (fat mass index females=3.5±0.5 kg/m2, males=2.4±0.5 kg/m2, P<0.001), while no differences were found in the extra cellular mass index (females=7.3±1.1 kg/m2, males=7.5±0.5 kg/m2; P=NS). There are no differences in systo-diastolic parameters between sexes. Greater relationships were found between left ventricular mass and body cells (r=0.829, r2=0.686, COV=401.5) compared to fat free mass (r=0.819, r2=0.675, COV=344.7). Left ventricular dimensions show higher values in males who also have the following body cell indexation (females=128.2±15.8 g/m2, males=143.4±14.7 g/m2; P<0.001)., Conclusions: Analyzing body composition in three compartments appears a solution that physiologically can explain some aspects of the athlete's heart. These results could be considered as preliminary data which can be used to create a new indexation.

Salinity is considered as one of the most important abiotic challenges that affect crop productivity. Plant hormones, including salicylic acid (SA), are key factors in the defence signalling output triggered during plant responses against environmental stresses. We have previously reported in peach a new SA biosynthetic pathway from mandelonitrile (MD), the molecule at the hub of the cyanogenic glucoside turnover in Prunus sp. In this work, we have studied whether this new SA biosynthetic pathway is also present in plum and the possible role this pathway plays in plant plasticity under salinity, focusing on the transgenic plum line J8-1, which displays stress tolerance via an enhanced antioxidant capacity. The SA biosynthesis from MD in non-transgenic and J8-1 micropropagated plum shoots was studied by metabolomics. Then the response of J8-1 to salt stress in presence of MD or Phe (MD precursor) was assayed by measuring: chlorophyll content and fluorescence parameters, stress related hormones, levels of non-enzymatic antioxidants, the expression of two genes coding redox-related proteins, and the content of soluble nutrients. The results from in vitro assays suggest that the SA synthesis from the MD pathway demonstrated in peach is not clearly present in plum, at least under the tested conditions. Nevertheless, in J8-1 NaCl-stressed seedlings, an increase in SA was recorded as a result of the MD treatment, suggesting that MD could be involved in the SA biosynthesis under NaCl stress conditions in plum plants. We have also shown that the plum line J8-1 was tolerant to NaCl under greenhouse conditions, and this response was quite similar in MD-treated plants. Nevertheless, the MD treatment produced an increase in SA, jasmonic acid (JA) and reduced ascorbate (ASC) contents, as well as in the coefficient of non-photochemical quenching (qN) and the gene expression of Non-Expressor of Pathogenesis-Related 1 ( NPR1 ) and thioredoxin H ( TrxH ) under salinity conditions. This response suggested a crosstalk between different signalling pathways (NPR1/Trx and SA/JA) leading to salinity tolerance in the transgenic plum line J8-1.

The aim of this study was to describe the changes in body composition, strength and sprint performance in response to an entire competitive season of football training supplemented with 2 inertial eccentric-overload training sessions a week in young male professional soccer players. Whole body and regional composition (assessed using dual-energy X-ray absorptiometry), power output in half-squat and 40-m sprinting performance were evaluated in fourteen players. The eccentric-overload training consisted of training sessions a week of 1-2 sets of 10 exercises of upper-body and core (Day 1) and lower-body (Day 2), during the entire competitive season (27 weeks). Whole body fat mass decreased (-6.3 ± 3.6%, ES = -0.99 ± 0.54) substantially while lean mass increased (2.5 ± 0.8%, ES = 0.25 ± 0.09), with some regional differences. There was a substantial increase in half-squat power output (from 3% to 14%, ES from 0.45 to 1.73) and sprint performance (from 1.1% to 1.8%, ES from -0.33 to -0.44), however performance changes were not correlated with changes in body composition. A combined soccer and eccentric-overload training program was able to promote positive changes in body composition and physical factors relevant to both on-field performance and injury prevention in elite soccer players., Competing Interests: A commercial company employed AB, RM and NT: ACF Fiorentina. This does not alter our adherence to PLOS ONE policies on sharing data and materials.

Cancer is the second leading cause of death, and the most common diagnosis among the general population is breast and colon cancer. Recently, an increasing number of new cases of invasive breast and colon cancer have been estimated, and more people die from these diseases. In addition to the genetic pattern, diet and lifestyle including smoking, alcohol consumption, and sedentary behaviour have also been identified as potential risks factors. Recent studies of cancer survivors have shown the beneficial effects of regular physical activity to reduce the prevalence of comorbidity, muscle atrophy, weight changes, reduced aerobic capacity, fatigue, depression, and reduced quality of life. Dedicated and individual programs are crucial for achieving the goals of improving quality of life and reducing comorbidities. A multidisciplinary approach is fundamental: lifestyle assessment, including estimating the level of physical activity, as well as nutritional habits, may be the first step. A periodic cardiovascular examination is crucial for detecting asymptomatic early myocardial failure. According to current ACSM guidelines, different levels of exercise (low-moderate 40% and moderate up to 60% of the maximal HR) may be prescribed, and patients enrolled may follow the exercise program if in the absence of contraindications. The current paper reports observations from our clinical practice and provides practical strategies that bridge contemporary, published guidelines into practice within a multi-disciplinary team working with cancer survivors in Italy.

In most woody fruit species, transformation and regeneration are difficult. However, European plum (Prunus domestica) has been shown to be amenable to genetic improvement technologies from classical hybridization, to genetic engineering, to rapid cycle crop breeding ('FasTrack' breeding). Since the first report on European plum transformation with marker genes in the early 90 s, numerous manuscripts have been published reporting the generation of new clones with agronomically interesting traits, such as pests, diseases and/or abiotic stress resistance, shorter juvenile period, dwarfing, continuous flowering, etc. This review focuses on the main advances in genetic transformation of European plum achieved to date, and the lines of work that are converting genetic engineering into a contemporary breeding tool for this species.

Introduction: The differences between the sexes in body composition are well established, although sports activity at an elite level seems to reduce them. The aim of this study is a comparison of the sexes in terms of athletes' body composition with a three-compartment model and a localized bioimpedance analysis in elite soccer players., Methods: 18 female and 18 male elite soccer players of the same age were matched (female=26.2±2.4, male 26.9±2.5; p=0.87). An assessment of body composition was performed through the integration of anthropometrics parameters, skinfold thickness and bioimpedance data. The evaluations were carried out in the morning on a group of athletes who were in a rested condition, having not exercised in the previous 12 hours and having fasted for breakfast., Results: A body composition assessment shows higher values in females for hip circumference/ height (female: 0.55±0.03, male: 0.52±0.02; p<0.01) and fat mass index (female: 3.7±0.7 kg/m 2 , male: 2.4±0.4 kg/m 2 ; p<0.001), while there is no difference between the genders in the extra cellular mass index (female: 7.1±1.2 kg/m 2 , male: 7.6±0.4 kg/m 2 ; p=0.11). A localized bioimpedance analysis describes well-defined differences in the thighs, while in the calves these differences are reversed for the reactance values., Conclusion: Sports activity seems to slightly reduce differences in the whole body. The different adaption at the same physical effort appears to be mainly related to cellular mass. This study shows for the first time localized bioimpedance values in female athletes., Level of Evidence: II b.

Objectives: to assess physical activity and overweight in a young population as factors associated with well-being., Design: cross-sectional study., Setting and Participants: data collection was performed in primary and secondary schools of a district located in Florence (Tuscany Region, Central Italy) by a validated questionnaire; 1,776 subjects were enrolled., Main Outcome Measures: physical activity levels and overweight., Results: an overweight or obese condition in 14,4% of the study population was observed. Vigorous physical activity prevailed in normal weight children, while the time spent in a sitting position was higher in overweight subjects., Conclusions: physical activity levels do not follow the guidelines for youth people. Educational programmes, also at schools, are recommended in order to prevent the risk of developing chronic diseases in adulthood.

The preparation and investigation of a free-standing membrane made from a composite of thermoresponsive poly(N-isopropylacrylamide) (pNIPAAm) and polystyrene nanoparticles (PS NP) with temperature-controlled permeability is reported. The method exploits the light-induced crosslinking of the photo-reactive pNIPAAm-based polymer and mechanical reinforcement of the membrane structure by the polystyrene nanoparticles. About micrometer thick layers were either directly attached to a gold surface or prepared as free-standing layers spanning over arrays of microfluidic channels with a width of about hundred microns by using template stripping. Diffusion of liquid medium, low molecular weight molecules, and large molecular weight proteins contained in blood through the composite membrane was observed with combined surface plasmon resonance (SPR) and optical waveguide spectroscopy (OWS). The swelling ratio, permeability, and nonspecific sorption to these composite membranes were investigated by SPR and OWS as a function of molecular weight of analyte, loading of PS NP in the composite film, and temperature. The authors show successful preparation of a defect-free membrane structure that acts as a thermoresponsive filter with nanoscale pores spanning over an area of several square millimeters. This membrane can be reversibly switched to block or allow the diffusion of low mass molecules to the sensor surface by temperature-triggered swelling and collapsing of the hydrogel component. Blocking of diffusion and low unspecific sorption of proteins contained in blood serum is observed. These features make this platform interesting for potential future applications in continuous monitoring biosensors for the analysis of low molecular weight drug analytes or for advanced cell-on-chip microfluidic studies.

Background: The aim of this study was to evaluate the lifestyle based on the physical activity and eating habits of young athletes., Methods: In order to measure physical activity and eating habits in young people, 922 young athletes between the ages of 8 to 18 have been analyzed in this study. The participants were all patients come to our Department for sport eligibility evaluation; we have asked them to complete an accurate questionnaire in order to assess their personal physical activity levels and their regular eating habits. Parents were invited to sign a letter explaining the aims of the study and were asked for permission on behalf of their child to take part on the study., Results: The eating habits and the physical activity levels of the young athletes observed, resulted improper. We have noticed that the 13.7% of the participants were overweight and obese, despite their practiced sport activity. Physical activity, without sport activity, resulted inadequate in 38.6% of participants: they did not practice regular physical activity., Conclusions: The results of the study clearly indicate that higher education is therefore necessary in order to promote a healthy lifestyle in terms of both eating habits and physical activity not only in young people, but also in parents and coaches of teams.

Despite the long-established importance of salicylic acid (SA) in plant stress responses and other biological processes, its biosynthetic pathways have not been fully characterized. The proposed synthesis of SA originates from chorismate by two distinct pathways: the isochorismate and phenylalanine (Phe) ammonia-lyase (PAL) pathways. Cyanogenesis is the process related to the release of hydrogen cyanide from endogenous cyanogenic glycosides (CNglcs), and it has been linked to plant plasticity improvement. To date, however, no relationship has been suggested between the two pathways. In this work, by metabolomics and biochemical approaches (including the use of [13C]-labeled compounds), we provide strong evidences showing that CNglcs turnover is involved, at least in part, in SA biosynthesis in peach plants under control and stress conditions. The main CNglcs in peach are prunasin and amygdalin, with mandelonitrile (MD), synthesized from phenylalanine, controlling their turnover. In peach plants MD is the intermediary molecule of the suggested new SA biosynthetic pathway and CNglcs turnover, regulating the biosynthesis of both amygdalin and SA. MD-treated peach plants displayed increased SA levels via benzoic acid (one of the SA precursors within the PAL pathway). MD also provided partial protection against Plum pox virus infection in peach seedlings. Thus, we propose a third pathway, an alternative to the PAL pathway, for SA synthesis in peach plants., (© The Author 2017. Published by Oxford University Press on behalf of Japanese Society of Plant Physiologists. All rights reserved. For permissions, please email: journals.permissions@oup.com.)

We have identified a gene (PpSAP1) of Prunus persica coding for a stress-associated protein (SAP) containing Zn-finger domains A20 and AN1. SAPs have been described as regulators of the abiotic stress response in plant species, emerging as potential candidates for improvement of stress tolerance in plants. PpSAP1 was highly expressed in leaves and dormant buds, being down-regulated before bud dormancy release. PpSAP1 expression was moderately induced by water stresses and heat in buds. In addition, it was found that PpSAP1 strongly interacts with polyubiquitin proteins in the yeast two-hybrid system. The overexpression of PpSAP1 in transgenic plum plants led to alterations in leaf shape and an increase of water retention under drought stress. Moreover, we established that leaf morphological alterations were concomitant with a reduced cell size and down-regulation of genes involved in cell growth, such as GROWTH-REGULATING FACTOR (GRF)1-like, TONOPLAST INTRINSIC PROTEIN (TIP)-like, and TARGET OF RAPAMYCIN (TOR)-like. Especially, the inverse expression pattern of PpSAP1 and TOR-like in transgenic plum and peach buds suggests a role of PpSAP1 in cell expansion through the regulation of TOR pathway.

Purpose: Lifestyle analysis is often used for primary and secondary prevention in many chronic metabolic diseases, including diabetes. Questionnaires are simple and common methods for first investigation risk of factors related to the perception of quality of life (QoL). The present study evaluates the feasibility to use questionnaires for first investigation of risk factors, and ascertain whether the results of such questionnaires are associated with the perception of QoL., Methods: Validated questionnaires from the international ACSM guidelines were used to study a cohort of 142 consecutive type 2 diabetes patients (mean age: 66.1 years ± 10.9)., Results: QoL perception was normal; BMI was compatible with overweight in 79.1% of subjects, and obesity in 20.9%. Cognitive abilities decreased with age and low consumption of dried fruit and legumes. There was evidence of a statistically significant association between BMI and QoL (rho = -0.18; p = 0.03)., Conclusions: Questionnaires are useful to assess lifestyle habits and highlight risks factors. Poor knowledge of patients' own chronic disease may contribute to a negative impact in diabetes.

Reversible actuating of surface plasmon propagation by responsive hydrogel grating is reported. Thermo-responsive poly(N-isopropylacrylamide)-based (pNIPAAm) hydrogel nanostructure was designed and tethered to a gold surface in order to switch on and off Bragg scattering of surface plasmons which is associated with an occurrence of a bandgap in their dispersion relation. pNIPAAm-based grating with a period around 280 nm was prepared by using photo-crosslinkable terpolymer and laser interference lithography and it was brought in contact with water. The temperature induced swelling and collapse of pNIPAAm hydrogel grating strongly modulates its refractive index (Δn~0.1) which leads to the reversible opening and closing of a plasmonic bandgap. The experiments demonstrate partial opening of a bandgap with the width of 12 nm at wavelength around 800 nm where SPR exhibited the spectral width of about 75 nm.

Regular physical exercise plays a role in improving cardiovascular and muscular fitness in many metabolic diseases. This study aims to verify any possible benefits, including the eventual influence on any associated risk factors, in a group of kidney transplant recipients after a short period of personalized training programs with mixed exercises. In January 2013, at the Sports Medicine Center of the University of Florence, Italy, we began studying a group of 20 kidney transplant recipients. After 6 months of exercise, they underwent Cardiopulmonary Test (CPET), ECG, skin fold, bioimpedance analysis and stress test for the lower and upper limbs. EF increased significantly from 63.38 ± 4 to 67.30 ± 5.9 with p < 0.05; the anaerobic threshold improved from 14.48 ± 6.3 to 20.24 ± 3.7 (p < 0.05) with good stress tolerance, estimated by CR10 scale; weight decreased significantly (70.06-65.03 kg) as did skin folds at pectoral level (p < 0.002). Upper limb muscular strength increased significantly (p < 0.005). Regular mixed exercise is a proposed program in post-transplant syndrome with the expectation of improving cardiovascular performance and enhancing exercise tolerance. Muscle strength improves with physical fitness with consequent reduction of risk factors linked to visceral fat. Proof of an eventual positive impact on other complex aspects associated with post-transplant metabolic syndrome will require a longer follow-up.

New plasmonic structure with actively tunable optical characteristics based on thermoresponsive hydrogel is reported. It consists of a thin, template-stripped Au film with arrays of nanoholes that is tethered to a transparent support by a cross-linked poly( N -isopropylacrylamide) (pNIPAAm)-based polymer network. Upon a contact of the porous Au surface with an aqueous environment, a rapid flow of water through the pores enables swelling and collapsing of the underlying pNIPAAm network. The swelling and collapsing could be triggered by small temperature changes around the lower critical solution temperature (LCST) of the hydrogel. The process is reversible, and it is associated with strong refractive index changes of Δ n ∼ 0.1, which characteristically alters the spectrum of surface plasmon modes supported by the porous Au film. This approach can offer new attractive means for optical biosensors with flow-through architecture and actively tunable plasmonic transmission optical filters.

Objective: The complement system plays a crucial role in the pathogenesis of inflammatory processes. The lectin pathway of the complement system is activated through the recognition of pathogens by soluble pattern recognition molecules (PRM), i.e., mannan-binding lectin (MBL), collectin-LK, and the ficolins. PRM are reportedly correlated to disease activity in rheumatoid arthritis (RA). The aim was to evaluate the pathogenic role of PRM in juvenile idiopathic arthritis (JIA)., Methods: We measured MBL, M-ficolin, H-ficolin, MBL-associated serine proteases (MASP) 1, MASP-2, MASP-3, and 2 alternative splice products, MBL-associated protein (MAp) 44 and MAp19, in plasma and synovial fluid (SF) of children with persistent oligoarticular (n = 109 in plasma, n = 38 in SF) and systemic JIA (n = 19 in plasma, n = 11 in SF). The concentrations of the proteins were measured by in-house time-resolved immunofluorometric assays., Results: We observed significantly higher levels of M-ficolin, MASP-1, MASP-2, and MASP-3 in plasma and SF from patients with systemic JIA compared with persistent oligoarticular JIA (p < 0.001). In paired samples of plasma and SF from 47 patients with oligoarticular and systemic JIA, we observed higher concentrations in plasma for both subtypes for 7 of the measured proteins while the reverse relationship was observed for MASP-3. M-ficolin and MASP-2 correlated to erythrocyte sedimentation rate, C-reactive protein, white blood cell count, and platelet count (p < 0.001). M-ficolin was in addition related to the number of active joints and inversely related to hemoglobin levels., Conclusion: Our results point to plasma M-ficolin and MASP-2 as inflammatory markers in JIA. The levels of all proteins are higher in plasma than in SF, except for MASP-3, indicating that MASP-3 may be produced locally in joints.

A protocol for Agrobacterium-mediated stable transformation of whole leaf explants of the apricot (Prunus armeniaca) cultivars 'Helena' and 'Canino' is described. Regenerated buds were selected using a two-step selection strategy with paromomycin sulfate and transferred to bud multiplication medium 1 week after they were detected for optimal survival. After buds were transferred to bud multiplication medium, antibiotic was changed to kanamycin and concentration increased gradually at each transfer to fresh medium in order to eliminate possible escapes and chimeras. Transformation efficiency, based on PCR analysis of individual putative transformed shoots from independent lines, was 5.6%. Green and healthy buds, surviving high kanamycin concentration, were transferred to shoot multiplication medium where they elongated in shoots and proliferated. Elongated transgenic shoots were rooted in a medium containing 70 μM kanamycin. Rooted plants were acclimatized following standard procedures. This constitutes the only transformation protocol described for apricot clonal tissues and one of the few of Prunus.

Unlabelled: PURPOSE Exercise therapy in patients with metabolic chronic disease produces several positive response. This study aims to verify the effects of fast walking associated to a resistance exercise to reduce cardiovascular risk factor., Methods: Two groups of subjects (10 cancer survivors and 19 hypertensive patients) were evaluated by 6-Minute Walking Test (6MWT), bioimpedance, the Sit & Reach Test (S&R) evaluate the flexibility, Handgrip and 30" Chair Test for muscular strength. Patients were tested before and after 3 months of regular physical exercise., Results: A significant change in anthropometric parameters was observed (BMI: T0 = 29.2±6.8, T3= 27.4±4.4 kg/m2 p<.001; waist circumference: T0=92.5±14.1, T3=92.1±12.8 cm, p<.05) in the hypertensive population. A predominant improvement of the cardiovascular parameters was observed in the cancer survivors (rest DBP T0=76.4±6.5, T3=72.2±7.1 mmhg p<.05; 6MWT: T0=487.8±116.0, T3= 525.6±117.3 m p<.05; S&R: T0= 0.4±7.4, T3=4.1±6.1 cm p<.05)., Conclusion: A combined aerobic and resistance exercise programme can improve cardiovascular risk factors in hypertensive subjects. The same programme induces improvement in exercise tolerance and flexibility variables in cancer survivors.

We explore the quantum dynamics of particles in a spatiotemporally driven lattice. A powerful numerical scheme is developed which provides us with the Floquet modes and thus enables a stroboscopic propagation of arbitrary initial states. A detailed symmetry analysis represents the cornerstone for an intricate manipulation of the Floquet spectrum. Specifically, we show how exact crossings can be converted into avoided ones, while the widths of these resulting avoided crossings can be engineered by adjusting parameters of the local driving. Asymptotic currents are shown to be controllable over a certain parameter range.

Purpose: The present study verified, using a validated questionnaire, the presence of unhealthy aspects of lifestyle and chronic degenerative conditions in a working community., Methods: A cohort from a working community in Italy was investigated using of the INRAN (Istituto Nazionale di Ricerca per gli Alimenti e Nutrizione) questionnaire dedicated to the eating habits and Physical Activity Stages of Change., Results: Most of the 93 subjects (56 females and 37 males, aged 42.0±0.7) recruited reported low levels of physical activity (70 subjects). Slightly more than 50% of the subjects undertook physical activity more than once a week, while 13% did it only once. Food intolerances were reported by 7 subjects (8%), with a high consumption of fruits, cereals and dairy products, low consumption of fish and alcohol, and meat consumption in the normal range. There was a high satisfaction in general quality of life., Conclusion: Questionnaire investigations play a role to identify the presence of degenerative chronic conditions in working communities. The self-reported perception of quality of life does not necessarily agree with the lifestyle habits found. Awareness of this aspect could be helpful to plan lifestyle interventions and promote healthy living habits.

Background: Mutations of the CYP17A1 gene cause 17α-hydroxylase deficiency (17OHD) resulting in 46,XY disorder of sex development, hypertension, hypokalemia and absent pubertal development. It is a rare, autosomal recessive form of congenital adrenal hyperplasia (CAH)., Patient: We report on a neonate with prenatally determined 46,XY karyotype. At 20 weeks of gestation, lack of development of male external genitalia was noticed. A phenotypically female child was born at 41 weeks of gestation., Results: Postnatal ultrasound revealed testes in both labia majora, an absence of uterus and normal adrenal glands. Steroid hormone analysis in serum revealed low basal levels of cortisol, testosterone and androstenedione in the presence of massively elevated corticosterone at the age of 2 weeks. The urinary steroid profile from spot urine showed excessive excretion of 17-desoxysteroids, decreased glucocorticoid metabolites and absent C19 steroids, thus proving 17OHD. Molecular analysis identified a novel mutation of the CYP17A1 gene: c.896T>A (p.I299N) in exon 5. Substitution with hydrocortisone was started. The child is raised as a girl and is developing well so far., Conclusion: Herein, we report the unusually early diagnosis of a newborn with the rare CAH form of 17OHD allowing an early start of treatment., (© 2014 S. Karger AG, Basel.)

To fortify the antioxidant capacity of plum plants, genes encoding cytosolic antioxidants ascorbate peroxidase (cytapx) and Cu/Zn-superoxide dismutase (cytsod) were genetically engineered in these plants. Transgenic plum plants expressing the cytsod and/or cytapx genes in cytosol have been generated under the control of the CaMV35S promoter. High levels of cytsod and cytapx gene transcripts suggested that the transgenes were constitutively and functionally expressed. We examined the potential functions of cytSOD and cytAPX in in vitro plum plants against salt stress (100 mm NaCl). Several transgenic plantlets expressing cytsod and/or cytapx showed an enhanced tolerance to salt stress, mainly lines C5-5 and J8-1 (expressing several copies of sod and apx, respectively). Transformation as well as NaCl treatments influenced the antioxidative metabolism of plum plantlets, including enzymatic and nonenzymatic antioxidants. Transgenic plantlets exhibited higher contents of nonenzymatic antioxidants glutathione and ascorbate than nontransformed control, which correlated with lower accumulation of hydrogen peroxide. Overall, our results suggest that transformation of plum plants with genes encoding antioxidant enzymes enhances the tolerance to salinity., (© 2013 Society for Experimental Biology, Association of Applied Biologists and John Wiley & Sons Ltd.)

In this study we examined the role of antioxidant metabolism in in vitro shoot multiplication. We generated transgenic plum plantlets overexpressing the cytsod and cytapx genes in cytosol under the control of the constitutive promoter CaMV35S. Three transgenic lines with up-regulated sod at transcriptional levels that showed silenced cytapx expression displayed an elevated in vitro multiplication rate. By contrast, a transgenic line harboring several copies of cytapx and with elevated APX enzymatic activity did not show any improvement in plant vigor, measured as the number of axillary shoots and shoot length. All of the lines with elevated micropropagation ability exhibited intensive H2O2 accumulation, monitored by 3,3'-diaminobenzidine (DAB) staining as well as by colorimetric analysis, providing direct in vitro evidence of the role of H2O2 and antioxidant genes in in vitro shoot multiplication., (Copyright © 2013 Elsevier GmbH. All rights reserved.)

This paper presents an improved photon path length probability density function method that permits simultaneous retrievals of column-average greenhouse gas mole fractions and light path modifications through the atmosphere when processing high-resolution radiance spectra acquired from space. We primarily describe the methodology and retrieval setup and then apply them to the processing of spectra measured by the Greenhouse gases Observing SATellite (GOSAT). We have demonstrated substantial improvements of the data processing with simultaneous carbon dioxide and light path retrievals and reasonable agreement of the satellite-based retrievals against ground-based Fourier transform spectrometer measurements provided by the Total Carbon Column Observing Network (TCCON).

We explore the nonequilibrium dynamics of noninteracting classical particles in a one-dimensional driven superlattice which is composed of domains exposed to different time-dependent forces. It is shown how the combination of directed transport and conversion processes from diffusive to ballistic motion causes strong correlations between velocity and phase for particles passing through a superlattice. A detailed understanding of the underlying mechanism allows us to tune the resulting velocity distributions at distinguished points in the superlattice by means of local variations of the applied driving force. As an intriguing application we present a scheme how initially diffusive particles can be transformed into a monoenergetic pulsed particle beam whose parameters such as its energy can be varied.

A high-throughput transformation system for plum has been developed using hypocotyl slices excised from zygotic embryos as the source of explants. The hypocotyl slices are infected in an Agrobacterium tumefaciens suspension and then cocultivated for 3 days in shoot regeneration ¾ MS basal medium supplemented with 9 μM 2,4-dichlorophenoxyacetic acid. Transgenic shoots are regenerated in a medium containing 7.5 μM thidiazuron and elongated in a medium containing 3 μM benzyladenine in the presence of 80 mg/L kanamycin in both media. Transformed shoots are rooted in ½ MS basal medium supplemented with 5 μM NAA and 40 mg/L kanamycin. The transgenic plants are acclimatized in a growth chamber and transferred to a temperature-controlled greenhouse. This protocol has allowed transformation efficiencies up to 42% and enabled the production of self-rooted transgenic plum plants within 6 months of transformation.

We develop and analyze a scheme to achieve both spatial and energetic focusing of an ensemble of neutral particles which is based on an oscillating billiard with frictional forces. The interplay of two competing mechanisms, acceleration due to collisions with the oscillating billiard walls and deceleration caused by friction, leads to the emergence of attractors in phase space. Their specific properties, i.e., spatial localization and energy spread, can be controlled and tuned by varying, e.g., the frequency of the time-dependent billiard.

We demonstrated very recently [Lenz, New J. Phys. 11, 083035 (2009)] that an ensemble of particles in the driven elliptical billiard shows a surprising crossover from subdiffusion to normal diffusion in momentum space. This crossover is not parameter induced, but rather occurs dynamically in the evolution of the ensemble. In this work, we consider three different driving modes of the elliptical billiard and perform a comprehensive analysis of the corresponding four-dimensional phase space. The composition of this phase space is different in the high-velocity regime compared to the low-velocity regime. We will show, among others, by investigating periodic orbits and probability distributions of laminar phases that the stickiness properties, which eventually determine the diffusion, are intimately connected with this change in the composition of the phase space with respect to velocity. In the course of the evolution, the accelerating ensemble thus explores regions of varying stickiness, leading to the mentioned crossover in the diffusion.

We investigate the quantum transmission through laterally driven single- and double-barrier systems in the nonlinear regime of strong driving. A broad parameter range is explored, distinguishing in particular between different frequency regimes. The applicability of an effective, time independent, potential description in the high-frequency regime is explored. Moreover, we analyze in detail the inelastic processes and their dependence on parameters, resonant tunneling, and photon assisted tunneling. For the single-barrier problem we address driving laws that differ from the purely sinusoidal one. In this context, we encounter reduced spatial and temporal symmetries and demonstrate the corresponding effects on quantum pumping. For the double barrier, the focus of our studies lies on the impact of the variation in the relative phase of the barriers on the transmission.

We explore the dynamics of noninteracting particles loaded into a phase-modulated one-dimensional lattice formed by laterally oscillating square barriers. Tuning the parameters of the driven unit cell of the lattice selected parts of the classical phase space can be manipulated in a controllable manner. We find superdiffusion in position space for all parameters regimes. A directed current of an ensemble of particles can be created through locally breaking the spatiotemporal symmetries of the time-driven potential. Magnitude and direction of the current are tunable. Several mechanisms for transient localization and trapping of particles in different wells of the driven unit cell are presented and analyzed.

The presence of marker genes conferring antibiotic resistance in transgenic plants represents a serious obstacle for their public acceptance and future commercialization. In addition, their elimination may allow gene stacking by the same selection strategy. In apricot, selection using the selectable marker gene nptII, that confers resistance to aminoglycoside antibiotics, is relatively effective. An attractive alternative is offered by the MAT system (multi-auto-transformation), which combines the ipt gene for positive selection with the recombinase system R/RS for removal of marker genes from transgenic cells after transformation. Transformation with an MAT vector has been attempted in the apricot cultivar 'Helena'. Regeneration from infected leaves with Agrobacterium harboring a plasmid containing the ipt gene was significantly higher than that from non-transformed controls in a non-selective medium. In addition, transformation efficiencies were much higher than those previously reported using antibiotic selection, probably due to the integration of the regeneration-promoting ipt gene. However, the lack of an ipt expression-induced differential phenotype in apricot made difficult in detecting the marker genes excision and plants had to be evaluated at different times. PCR analysis showed that cassette excision start occurring after 6 months approximately and 1 year in culture was necessary for complete elimination of the cassette in all the transgenic lines. Excision was confirmed by Southern blot analysis. We report here for the first time in a temperate fruit tree that the MAT vector system improves regeneration and transformation efficiency and would allow complete elimination of marker genes from transgenic apricot plants by site-specific recombination.

A detailed analysis of the classical nonlinear dynamics of a single driven square potential barrier with harmonically oscillating position is performed. The system exhibits dynamical trapping which is associated with the existence of a stable island in phase space. Due to the unstable periodic orbits of the KAM structure, the driven barrier is a chaotic scatterer and shows stickiness of scattering trajectories in the vicinity of the stable island. The transmission function of a suitably prepared ensemble yields results which are very similar to tunneling resonances in the quantum mechanical regime. However, the origin of these resonances is different in the classical regime.

We report Agrobacterium tumefaciens-mediated transformation of two Prunus salicina varieties, 'Angeleno' and 'Larry Anne', using a modification of the hypocotyl slice technique previously described for P. domestica. Regeneration rates on thidiazuron (TDZ) and indole-3-butyric acid (IBA) supplemented Murashige and Skoog (MS) media reached 11% for 'Angeleno' and 19% for 'Larry Anne' hypocotyl slices. Transformation using Agrobacterium tumefaciens GV3101 harboring a plasmid with the neomycin phosphotransferase II (nptII) and the green fluorescent protein (gfp) genes produced ten independent lines, six from 'Angeleno' and four from 'Larry Anne', representing transformation efficiencies of 0.8 and 0.3%, respectively, relative to the initial number of hypocotyl slices. Plants of six lines were found to produce the transgene encoded mRNAs. DNA blotting demonstrated the presence of transgene sequences in trees from five lines after 18 months of growth in the greenhouse.

A protocol for Agrobacterium-mediated stable transformation for scored, whole leaf explants of the apricot (Prunus armeniaca) cultivar Helena was developed. Regenerated shoots were selected using a two-step increased concentrations of paromomycin sulphate. Different factors affecting survival of transformed buds, including possible toxicity of green fluorescent protein (GFP) and time of exposure to high cytokine concentration in the regeneration medium, were examined. Transformation efficiency, based on PCR analysis of individual putative transformed shoots from independent lines was 5.6%, when optimal conditions for bud survival were provided. Southern blot analysis on four randomly chosen PCR-positive shoots confirmed the presence of the nptII transgene. This is the first time that stable transformation of an apricot cultivar is reported and constitutes also one of the few reports on the transformation of Prunus cultivars.

Regeneration and transformation systems using mature plant material of woody fruit species have to be achieved as a necessary requirement for the introduction of useful genes into specific cultivars and the rapid evaluation of resulting horticultural traits. Although the commercial production of transgenic annual crops is a reality, commercial genetically-engineered fruit trees are still far from common. In most woody fruit species, transformation and regeneration of commercial cultivars are not routine, generally being limited to a few genotypes or to seedlings. The future of genetic transformation as a tool for the breeding of fruit trees requires the development of genotype-independent procedures, based on the transformation of meristematic cells with high regeneration potential and/or the use of regeneration-promoting genes. The public concern with the introduction of antibiotic resistance into food and the restrictions due to new European laws that do not allow deliberate release of plants transformed with antibiotic-resistance genes highlight the development of methods that avoid the use of antibiotic-dependent selection or allow elimination of marker genesfrom the transformed plant as a research priority in coming years.

We present the case of a silicotic patient, with recurrent febrile episodes during the past three years, who was hospitalized for the study of prolonged fever. In addition to compliance with the criteria of fever from unknown origin (FUO), the patient presented semiology and echocardiogram compatible with infectious endocarditis. Later on, he developed a severe aortic failure refractary to medical treatment and hence, he underwent surgery. The surgical intervention showed an abscess in the aortic root, which resulted to be of a tuberculous origin and an intact aortic valve. The review of the echocardiographic study allowed the detection of an image compatible with aortic abscess. We comment on the characteristic of this rare entity and the peculiarities of this case, which fulfill the criteria of what has been recently named recurrent or episodic FUO and among whose causes Mycobacterium tuberculosis had not been detected up to now.

Unlabelled: New techniques have influenced the attitude to the autopsy and contribute to a de-emphasis on the importance of post-mortem examination. Since 1990, new Danish legislation has provoked a dramatic fall in the autopsy rate, which had already declined from 45% in 1970 to 35% in 1980. In the first half of 1990 the rate was 24% in the second half of that same year it had fallen to 16% (, Source: The Danish National Institute of Health, 1992). The clinicians now seem to manage without the autopsy to confirm or correct their daily diagnostics. They also seem to be of the opinion that they do well without this "final checklist". The autopsy, however, is still an important tool in understanding, correcting and improving future diagnosis. Therefore, post-mortems should again be carried out as a matter of course and common practice. The following proposals are all aimed at obtaining a higher autopsy rate: The 1990 legislation on autopsy should be changed so that permission to perform a post-mortem can be given in due time, before the supposed death, preferably by the patient himself and obviously with the right to a subsequent change of mind. It is of great importance that the Public Health Service informs both the public and health workers in general about the nature and importance of the autopsy. Likewise, doctors and health workers in general should be educated in how best to give information to patients. Pathologists should, through a more uniform and exact practice, encourage the clinicians to a renewal of the close collaboration concerning the facts revealed by the autopsy, both in their everyday practice and in scientific projects in general.

78 female and 31 male Danish gallstone cases found by autopsy were studied. The mass of the gallstone(s) from each patient was recorded and the stones were ground. The content of cholesterol in the mixed material from each patient was determined, using the Liebermann-Burchard reaction. The ratio Gallstone(s) ash-mass/Gallstone(s)-mass was determined, and where this ratio exceeded 0.10, calcium and phosphate determinations were carried out. Subjective description of the stones as 'mixed stones' or 'pigment stones' was substituted by visual comparison of the ground gallstones with a 'grey scale' (black was given the highest value). The values of this quantity and the ash values generally increased with a decreasing content of cholesterol. The content of cholesterol in the gallstones increased with increasing stone mass. The chemical composition of gallstones found by surgery seems to differ from that of gallstones found by autopsy.