Search

Orientador: Walter August Hadler Tese (doutorado) - Universidade Estadual de Campinas, Instituto de Biologia Resumo: Não informado Abstract: Not informed Doutorado Doutor em Ciências

The present paper deals on the histological description of the hake ovary made on the basis of gonad observations of 394 females during the period April 1966 March 1967. The material was obtained from weekly sampling of commercial catches carried out at the Institute of Marine Biology (Prov. Buenos Aires, Argentina). The anatomical and histological description of the standard ovary and the adopted terminology are given. The maturation process is divided into five periods, from ovocyte formation to yolked ovocyte formation, with its histological description. Ovary changes are analyzed on detail. The following conclusions were outlined: 1. Analysis demonstrated that although some specimens were totally spawned others, at the end of the spawning period, retaining a great number of ovocytes in different maturity stages. Therefore, postspawners have been classified as follows: Postspawned II : This stages is characterized by the empty ovarian structure, with ovocytes in stage II, which will remain in the resting phase untill next spawning season. Postspawned III and IV: Their main characteristics are: tissue destruction, bloody residuals and remaining ovocytes in stage III and IV, respectively. 2. Some transformations were found in ovaries of postspawned III and IV. They are classified as follows, according to its origin and structure: Developed from follicular cell membrane – a) Glandular formations, b) Epiteloid formations - Originated from remaining ovocytes, c) Ovocyte disintegration, d) Ovocyte with follicular cell infiltration. 3. All those structures derived from postpawners III and IV have a temporary character and will be reabsorbed. Their presence delay the recuperation of the organ and its reproductive functions. Consequently, the possibility of those structures acting as control mechanisms is suggested. 4. Transformations pointed out in paragraph Nº 2 prevent the possibility of consecutive spawning originated from the remaining ovocytes (II and IV). 5. No structures originated from postspawners III and IV were found during summer season. 6. Reproductive cycle of hake has been described monthly. It was observed that maturing ovaries predominate in summer (November-December).

Historia clínica. El día 14 de diciembre del año próximo pasado,recomendada por una distinguida dama de la sociedad, entró alservicio de mujeres, salón de caridad, a cargo del doctor Alejandro Bernal Jiménez, del Hospital San José del Líbano, Departamento del Tolima, la enferma L. S., de 70 años de edad natural de Chiquinquirá, hija de Manuel Sánchez y Joaquina Cortés.

"Hembras de Triatoma barberi fueron infectadas experimentalmente con Trypanosoma cruzi a partir del segundo estadio ninfal, con el fin de detectar posibles alteraciones en los ovarios y en las proteínas del vitelo del huevo. En las hembras infectadas y en las hembras testigo, se tomaron en cuenta los cambios morfológicos que se presentaron en los ovarios durante su maduración, así como los patrones electroforéticos de las proteínas del vitelo del huevo. La morfología de los ovarios de Triatoma barberi no fue afectada por la infección con Trypanosoma cruzi; los patrones de crecimiento de los ovarios hasta su madurez fueron los mismos en las hembras testigo y en las infectadas. Las proteínas del vitelo del huevo, en ambos grupos de hembras, no presentaron diferencias en los patrones electroforéticos."

O estudo da fisiologia reprodutiva dos animais de companhia tem uma grande importância tanto a nível da sua própria espécie, como também para estudos científicos de patologias do homem. O estudo da atrésia folicular na cadela e na gata ainda é muito rudimentar. No entanto, a atrésia folicular pode estar presente em qualquer fase folicular e em qualquer fase do ciclo éstrico em mamíferos. Este trabalho teve como objectivo descrever o processo de atrésia folicular ao longo da foliculogénese e nas fases do ciclo éstrico em cadelas e gatas, com a utilização da técnica laboratorial TUNEL. Para a realização deste trabalho, foram analisados ovários de 7 cadelas e 8 gatas com idades compreendidas entre os 5 meses e os 2 anos, distribuídos por fase de ciclo éstrico. Neste trabalho foi possível a observação de marcação de folículos em qualquer fase do ciclo éstrico e da foliculogénese. No entanto, as cadelas e as gatas em fases de diestro e anestro apresentaram uma maior evidência de marcação nas várias fases da foliculogénese. As células com maior marcação em todas as amostras foram as células da granulosa nas várias fases de foliculogénese. Contudo, foi também possível observar marcação em oócitos em folículos primordiais em gatas e em folículos pré-antrais nas cadelas. Também se observou marcação em algumas células da teca interna em folículos pré-antrais e antrais em cadelas e em folículos antrais em gatas. Em alguns folículos antrais, a marcação era evidente nas células da granulosa e nas zonas de cumulus oophorus e corona radiata. Com a realização deste estudo, foi possível concluir que a apoptose está presente em todas as fases da foliculogénese na cadela e na gata e em qualquer fase do ciclo éstrico, como referido em estudos anteriores de outras diferentes espécies., The study of the reproductive physiology of companion animals is of great importance both at the level of their own species and for scientific studies of human pathologies. The study of follicular atresia in the female dog and cat is still very rudimentary. However, follicular atresia may be present at any follicular stage and at any stage of the mammalian oestrus cycle. The aim of this study was to describe the follicular atresia along the folliculogenesis and in the stages of the oestrus cycle in female dogs and queens, using the TUNEL laboratory technique. For the accomplishment of this work, ovaries of 7 female dogs and 8 queens with ages between 5 months and 2 years, distributed by phase of the cycle were analysed. In this work it was possible to detect the positive labeling on follicles at any stage of the oestrus cycle and folliculogenesis. However, both species during diestrus and anestrus phases presented greater evidence of positive labeling in the various phases of folliculogenesis. The cells with the highest positive labeling in all samples were the granulosa cells in the various phases of folliculogenesis. However, it was also possible to observe positive labeling in oocytes in primordial follicles in queens and in preantral follicles in female dogs. Positive labeling was also observed in some theca interna cells in preantral and antral follicles in female dogs and in antral follicles in queens. In some antral follicles, positive labeling was evident in granulosa cells and in the areas of cumulus oophorus and corona radiata. With the realization of this study, it was possible to conclude that apoptosis is present in all phases of folliculogenesis in both species and in any phase of the cycle, as reported in previous studies of others different species., Orientação: Daniel Murta