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Valorization of biomass based on feedstock has become an urgent necessity in terms of sustainability. Materials produced from fossil fuels will eventually run out. Therefore, it is inevitable to evaluate particularly food and agricultural wastes that have no economic value or even threaten the environment. In this study, olive tree (Olea europeae) has been investigated with respect to its by-product such as leaf. Several conventional solvents (ethanol, methanol, acetonitrile, and acetone solutions) were used to obtain an extract rich in total phenolics (TPI), flavonoids (TFI), and antioxidant activity (AA) by means of microwave-assisted extraction (MAE). On the other hand, 3-factor and 3-level central composite design (CCD) was applied via the response surface method (RSM) to determine the optimum experimental conditions of the parameters that were effective on a small number of experiments. Generally, solvent volume was the most effective parameter for each solvent system (P < 0.05). The process parameters of the extraction system were selected as microwave power (150–250 W), time (0.5–1.5 min), and solvent volume (50–100 mL). The best findings for TPI, TFI, and AA were found 10.45-mg GAE/g DL, 9.69-mg CE/g DL, and 96.34% under the optimal conditions (230 W, 1.5 min, and 63.16 mL of 30% acetonitrile solution).

A new wild-type Ganoderma resinaceum isolate was cultivated on glucose-enriched liquid cultures with olive mill wastewaters (OMWs) in initial phenolic compounds concentrations 0.0 (control), 0.5, 0.8, and 1.5 g/l. The effect of the fungus on the reduction of phenolics and color was assessed, whereas biomass production, glucose consumption, intra-cellular (IPS) and extra-cellular (EPS) polysaccharides biosynthesis, antioxidant activity of the biomass, and laccase synthesis were monitored. Results showed that significant phenolic reduction (94.5%) and decolorization (76.5%) occurred, 14.6 g/l of biomass was produced, glucose was almost totally consumed, EPS were produced in sufficient amounts (0.79 g/l), whereas the presence of OMWs enhanced the synthesis of IPS (maximum absolute values 4.0-5.2 g/l corresponding to 35-42% w/w). Kinetic analysis demonstrated that EPS and IPS values fluctuated with time, regardless of the available amount of glucose in the media, showing a maximum in the 17th day of culture. Laccase was highly synthesized in the middle of the fermentation, reaching the maximum value of 14 U/ml. Little growth was however observed at 1.5 g/l phenolics. Strong correlation between total phenolic content and free radical scavenging activity has been noticed in the methanolic extracts of the mycelium. Results strongly suggest the potentiality of G. resinaceum utilization in the OMW waste treatment.

The effect of replacing wheat straw and a portion of commercial concentrate with olive twigs and leaves treated with urea and molasses on nutrient digestibility, growth performance and blood variables was determined. Forty‐two Shami goat kids (20 ± 2.47 kg live weight and 133 ± 5.44 days of age) were randomly allotted to three dietary treatment groups, control (75% commercial concentrate and 25% wheat straw), OTU (62.3% commercial concentrate + 37.7% olive twigs and leaves treated with 4% urea for 14 days), OTUM (51.5% concentrate mixture + 48.5% olive twigs and leaves treated with 4% urea for 14 days then sprayed by 10% molasses). Cost (SYP/t) of control, OUT and OTUM was 12,600, 10,066 and 10,106 respectively. The study consisted of a growth trial of 100 days and a digestibility trial of 12 days (7 days of adaptation and 5 days of urine and faeces collection). Serum urea, total protein, albumin, alanine aminotransferase activity, aspartate aminotransferase activity, glucose, cholesterol, creatinine, haemoglobin and packed cell volume were determined. Daily dry matter intake, digestibility of crude protein, ether extract and nutrient detergent fibre did not significantly differ among the dietary treatments (p > .05). Nitrogen intake, nitrogen excretion in urine and faeces and nitrogen balance was not significantly affected (p > .05) by the dietary treatment. The dietary treatment did not have any significant effect on average daily gain, feed conversion ratio or blood variables (p > .05). Olive twigs treated with 4% urea and OTUM replaced 37.7% and 48.5% of conventional diets of growing Shami goat kids without negative effects on growth performance nor health and potentially reduced fattening costs for farmers in olive‐livestock mixed systems by 19.8 and 20%, respectively. OUT is technically simpler than OTUM, thus, it could replace the conventional fattening ration of Shami male goat., Shami goat were fed on Olive twigs treated with 4% urea and 10% Olive twigs treateb with 4% urea replaced 48.5% of the ration without negative effect on growth performance This replacement did not affect goat health but decreased feeding cost by 20%.

Bu çalışmada trafik yoğunluğundan kaynaklanan ağır metal konsantrasyonunun izlenmesinde zeytin ağacının (Olea eurpaea L.) biyomonitör olarak kullanılma potansiyeli araştırılmıştır. Bu amaçla Artvin ili kent merkezinde trafik yoğunluğunun farklı olduğu altı farklı istasyondan toplanan toprak, yaprak, meyve ve kabuk örneklerindeki % kükürt miktarı, elementel analiz cihazı ile Cr, Ti, Fe, Ni, Co, Cu, Zn, Pb ve Al içerikleri ise ICP-OES ile belirlenmiştir. Analiz ölçümlerinin doğruluğu toprak örnekleri için LGC 6187 ve bitki örnekleri için ERM-CD 281 standart referans madde kullanılarak test edilmiştir. Elde edilen ölçüm sonuçlarındaki farklılıklar ise ANOVA testi kullanılarak belirlenmiştir. Elde edilen sonuçlara göre; toprak, yaprak, meyve ve kabukta yüzdesel olarak en yüksek kükürt değeri trafik yoğunluğunun en fazla olduğu noktalarda tespit edilmiştir. Bitki ve toprak örneklerindeki Cr, Ti, Fe, Ni, Co, Cu, Zn, Pb ve Al elementleri için ağır metal konsantrasyonları yapılan diğer çalışmalar ve yönetmeliklerle karşılaştırılmış ve tüm metallerin sınır değeler içerisinde bulunduğu gözlenmiştir. Yapılan istatiksel çalışmalarda; noktalar arasında %95 güven düzeyinde anlamlı bir fark olduğu belirlenmiştir. Ayrıca trafiğin yoğun olduğu alanlardan uzaklaştıkça toprak, yaprak, meyve ve kabuğun metal konsantrasyonlarında azalma olduğu tespit edilmiştir. Bu durum zeytinin trafik kaynaklı kirliliğin belirlenmesinde biyomonitör olarak kullanılabileceğini göstermektedir.

Silver nanoparticles are applied in a variety of medical and non-medical fields due to their unique properties. Researchers are keen on synthesizing silver nanoparticles from biological sources as they are environmentally sound. Plants are a good option as they are abundant in nature and are rich in active metabolites that help in the reduction of silver ions. In this study, we have synthesized silver nanoparticles using olive fruit extract which were characterized by UV–visible spectroscopy, Fourier Transform Infrared spectroscopy (FTIR), High Resolution Scanning Electron Microscopy (HR-SEM) and zeta potential analysis which confirmed the formation of spherical, nano-sized particles. The efficacy of synthesized silver nanoparticles against the growth and biofilm formation of major oral pathogens Streptococcus mutans and Candida albicans was demonstrated. The study revealed that olive fruit extract is an excellent producer of silver nanoparticles with potential activity against oral pathogens.

The present study aims to ascertain the potency of Olea europaea L. and Argania spinosa (L.) Skeels leaves extracts in natural phenolic compounds of variable antioxidant properties. The obtained extracts were investigated for their total phenolic and total flavonoid content, total antioxidant activity (TAC), as well as evaluated for their ferric reducing capacity (FRAP), β-carotene bleaching inhibition capacity, and for the radical scavenging activity through the extent of their abilities to scavenge the DPPH• and ABTS+ radicals. Antioxidant contents and capacities were expressed as mg/g DW, IC50, and as (%) inhibition in percentage. Total phenolic content was ranged between 378.10 ± 2.05 to 22.51 ± 0.07 mg/g DW in argan samples and from 226.50 ± 1.32 to 15.02 ± 0.04 mg/g DW in olive samples, expressed as gallic acid equivalents and shown in the decreasing order: methanolic extracts > aqueous extracts > ethyl acetate extracts. Moreover, for both plant species; the highest reducing and antiradical power as well as the greatest total antioxidant capacities, were obtained by the methanolic leave extracts. Whilst the most distinguished β-carotene bleaching inhibition was observed for aqueous extracts of olive (90.67%) and argan (87.39%) macerated leaves. With this highly revealed antioxidant power of the extracts, it was significantly linked to the important correlations between the in vitro executed assays and the total phenolic (TP) and total flavonoid (TF) contents detected. Based on the obtained results, it could be concluded that argan and olive leaves display promising antioxidant capacities to potentially serve as rich sources of phenolic compounds with variable antioxidant properties.

Olive growing in Palestine plays an important role at social and economic levels. Nevertheless, the quality of olive oil produced in the country has not been fully addressed. This study examined oil content, peroxide values, acid values, fatty acid profile, and total phenolic content for old olive trees located in different climatic regions in Palestine during the years 2008-2010. Oil content was determined using both Soxhlet and Abencor systems. Acid and peroxide values were determined using standard methods. Total phenolic content was determined using the Folin-spectrophotometric method. Gas chromatography was used to analyze the main fatty acids found in olive oil e.g., palmitic, palmitoleic, stearic, oleic, linoleic. Different ratios indicating olive oil quality were also determined e.g., sum ratio of unsaturated fatty acids to the sum of saturated fatty acids; ratio between the sum of monounsaturated fatty acids to the sum of polyunsaturated fatty acids, and the ratio between the oleic to linoleic fatty acids. Significant differences were found between geographic regions for the overall studied oil parameters. Wide variation ranges were obtained for fatty acids in the different West Bank locations in the three years. The major fatty acids in the olive oil samples were found to be oleic, palmitic, stearic, linoleic, and palmitoleic acids. The oil samples were found to contain more oleic acid and less linoleic and linolenic acids that is, more monounsaturated than polyunsaturated fatty acids. Total phenolic content was found to range from 125.0-978.0, 207.4-763.8, and 103.0-747.6 mg/kg in 2008, 2009, and 2010, respectively. The acidity percentage was in the range of 0.10%-1.05%, 0.11%-1.29%, and 0.10%-1.91% in 2008, 2009, and 2010, respectively. Peroxide values ranged from 2.26-13.1, 2.94-14.95, and 2.49-17.21 in 2008, 2009, and 2010, respectively.

Graphical abstract The thiolase enzyme OleA initiates the biosynthesis of β-lactone natural products and membrane hydrocarbons via acyl-Coenzyme A (CoA) substrates, but this study showed that p-nitrophenyl alkanoates can substitute, yielding new insights into the enzyme mechanism and providing a cheaper and more available starting substrate., The OleA enzyme is distinct amongst thiolase enzymes in binding two long (≥C8) acyl chains into structurally-opposed hydrophobic channels, denoted A and B, to carry out a non-decarboxylative Claisen condensation reaction and initiate the biosynthesis of membrane hydrocarbons and β-lactone natural products. OleA has now been identified in hundreds of diverse bacteria via bioinformatics and high-throughput screening using p-nitrophenyl alkanoate esters as surrogate substrates. In the present study, p-nitrophenyl esters were used to probe the reaction mechanism of OleA and shown to be incorporated into Claisen condensation products for the first time. p-Nitrophenyl alkanoate substrates alone were shown not to undergo Claisen condensation, but co-incubation of p-nitrophenyl esters and CoA thioesters produced mixed Claisen products. Mixed product reactions were shown to initiate via acyl group transfer from a p-nitrophenyl carrier to the enzyme active site cysteine, C143. Acyl chains esterified to p-nitrophenol were synthesized and shown to undergo Claisen condensation with an acyl-CoA substrate, showing potential to greatly expand the range of possible Claisen products. Using p-nitrophenyl 1-13C-decanoate, the Channel A bound thioester chain was shown to act as the Claisen nucleophile, representing the first direct evidence for the directionality of the Claisen reaction in any OleA enzyme. These results both provide new insights into OleA catalysis and open a path for making unnatural hydrocarbon and β-lactone natural products for biotechnological applications using cheap and easily synthesized p-nitrophenyl esters.

Salt stress is a major limiting factor for the growth and productivity of plants worldwide. This factorial experiment within a randomized complete block design with three replications was carried out to study the influence of foliar spray with three levels of silicon (K2SiO3) (0, 100, and 200 mg. L−1) on the effect of four concentrations of water salinity (1.6, 3, 6, and 9 dS/m−1) in two olive (Olea europaea L.) cultivars (bashiki and Shami). Most of the seedling cultivars were one-year-old. The results showed that the Bashiki cultivars were significantly better than Shami by giving the best values for the height of the seedlings, leaves number, and percentage of the mineral element. It was observed that treatment with 9 dS/m−1 is negatively influenced in the two varieties in vegetative properties and the percentage of N, P, K but an increase of sodium, chloride, and proline in leaves. Moreover, treatment of silicon (100 mg L−1) reduced the salinity effects on the olive seedlings. The results of the interaction between the study factors showed that plants treated with the combination of (1.6 dS/m−1+ silicon (100 mg L−1)) led to an increase in all studied trait. We found considerable differences between the two cultivars. silicon treatments can reduce the reverse effects of salinity on olive cultivars.

In this study, the combined effect of different packaging materials (transparent PET, transparent glass, glass-PET bottle and tin), some aromatic herbs (thyme, rosemary, sage and olive leaf) and also their essential oils (thyme, rosemary and sage) on fatty acid composition of virgin olive oil was investigated during storage period. The initial amounts of the main fatty acids as oleic, palmitic and linoleic acids were determined as 72.89%, 11.89% and 8.96%, respectively. The addition of aromatic plants and essential oils did not effect the fatty acid profile. Also, packaging materials had a minor influence on fatty acids. In the 6th month of storage, the oleic acid contents of olive oils showed the increase in all of samples. The highest increase was observed in olive oil stored in glass-PET (74.30-75.01%), followed by stored in glass bottle (73.41-74.82%). Generally, during the storage, the differences of fatty acid contents were in minor level. The fatty acid composition of olive oils stored under different essential oil and extract concentrations showed partial differences depending on the extract type and concentration.

The genetic relationships among six different olive (Olea europaea L.) genotypes were determined through ten ISSR primers by obtaining informative profiles using five of them. The studied cultivars ‘Chondrolia Chalkidikis’, ‘Karidolia,’ ‘Prodromos,’ ‘Kalamon,’ ‘Koroneiki,’ and ‘Leucocarpa’ are widespread and grown in Chalkidiki and Northern Greece. By analysis of the amplification, a total of 34 bands were observed, and it was noticed that 26 of them were polymorphic, showing the high efficiency and reliability of the markers used. The highest number of ISSR bands detected for primer E4 was 10 (8 polymorphic). The calculated percentage of polymorphism was 76.5. A cluster analysis was performed based on the molecular characterization results, and a dendrogram was constructed using average linkage. The current genotyping presents distinctive profiles useful for proving olive planting material's cultivar and clonal identity.

Leishmaniasis is a major public health problem globally. Visceral leishmaniasis is known to be fatal if left untreated, while cutaneous leishmaniasis is the most neglected. The first-line treatment of leishmaniasis is based on pentavalent antimonial drugs which are expensive, requiring inpatient treatment and toxic. The Plants containing active compounds against other protozoan diseases may offer alternatives against leishmania parasites. This study determined the in vitro antileishmanial activity of Olea europaea, Kigelia africana, Terminalia mollis, Croton macrostachyus and Bridella micrantha extracts. The plant samples were dried, pulverized into fine powders and extracted using ethanol at the Center for Traditional Medicine and Drugs Research, KEMRI. The in vitro assays were carried out at the Leishmania laboratory, Centre for Biotechnology Research and Development, KEMRI. In in- vitro assays the inhibitory concentrations (IC50) and Minimum Inhibition Concentration (MIC) on L. major promastigotes, percentage rates of macrophages infected by amastigotes and cytotoxicity on Vero cells were determined. For each parameter analyzed, differences among treatment groups exposed to different drugs were tested by logistic regression. Results showed that promastogote and amastigote growth inhibition was significantly affected by the crude extracts from the plants (P < 0.05) after 24 hours of exposure where the most effective drug was the standard drug (Amphotericin B) while among the crude extracts of the herbal drugs, T. mollis was the most effective against amastigote followed by C. macrostachyus while O. europaea was the least effective. Mammalian cell viability was significantly affected by the various test compounds (P < 0.05) after 24 hours exposure where % cell viability of herbal drugs, B. microstachyus, O. africana resulted to the most toxic effects by reducing the % cell viability to less than 50%. The study recommends the use of T. mollis in management of leishmaniasis in areas they occur. Further analysis of the active compounds that that affect efficacy of the plant extracts is advised.

The cellular response to stress also includes the accumulation of reactive oxygen species (ROS). When in excess, these second messengers can cause significant detrimental biochemical changes, such as stress-induced lipid peroxidation. In plants, a powerful endogenous antioxidant system is based on the enzymatic modification of redox-sensitive cysteines, carried out for example, by thioredoxin proteins. In this work, we report the cloning and characterization of a thioredoxin gene from olive (Olea europaea L.), which was named Oe-Trx h I. We also performed an expression analysis in drupes following biotic stress, as a first step towards the definition of its possible defensive role against the olive fruit fly [Bactrocera oleae (Rossi)]. The data indicate that the cloned thioredoxin h is a potential member of the biotic stress response in drupes and suggest that Oe-Trx h I may be a component active towards an increased resistance against oxidative stress. This work opens the way to further studies to assess the protective role of this gene against ROS accumulation and lipid peroxidation in drupes following larval feeding.

Plants have always represented a great resource of bioactive compounds. Olea europaea is considered one the most important fruit trees of the entire Mediterranean basin and its use in traditional m...

A strategy to improve productivity in olive orchards is the evaluation of cultivars that respond better to the environmental growing conditions The objective of this experiment was to evaluate the yield potential and oil quality of five olive cultivars (Arbequina, Coratina, Manzanilla, Arbosana and Mission) in two regions of Mexico. The experiment was carried out during two consecutive years 2018 and 2019. At Northwest Region the five olive cultivars were planted in 2013 at a spacing of 10 x 5 m (having 200 trees ha-1) and Central Region olive cultivars were planted in 2013 at spacing of 6 x 6 m (278 trees ha-1) and both locations under drip irrigation systems. The variables evaluated were: yield, fruit weight, oil content and oil quality (acidity, peroxide value and absorbance K232 and K270). The experiment was analyzed using a randomized complete block design with four replications. Our results showed high differences between locations, Arbequina was the cultivar with the highest yield at the fifth and sixth years of planting with an average of 48.8 kg tree-1 at Northwest Region and only 15.5 kg tree-1 at Central Region. The cultivar with the highest oil content in the Northwest Region was Manzanilla with an average of 12.3% and the lowest for Arbequina with only 9.3%, while at Central Region the cultivar with the highest oil content was Coratina with 20.2% and the lowest in Manzanilla with 16.1%. Extra virgin olive oil was obtained in all the cultivars in both locations according to chemical analysis. Finally, it is necessary to continue with the evaluation of other varieties with resistance to high temperatures at Northwest Region and resistance to frost at Central Region.

Summary A total of seventeen olive ecotypes were identified in the oasis region of Degache (Tozeur, Tunisia). The following physicochemical parameters of their oils were studied: acidity, refraction index, polyphenol, chlorophyll and carotene contents, oleic, palmitic and linoleic acid levels and the UV absorbance (K232 and K270). The acidity, K232 and K270 parameter values were used to classify most of the olive oils considered as ‘extra virgin’. The highest carotene, chlorophyll and polyphenol contents of the olive oils examined were found to be 13.60 ppm, 9.10 ppm and 796 ppm, respectively. The oleic acid level of the olive oils considered ranged between 47% and 73%, whereas the palimtic acid level ranged between 11.8% and 23%, and the linoleic acid level ranged between 8.8% and 22.5%. Therefore, the fatty acid composition of the most olive oils considered was in keeping with the IOC norms. The Pearson correlation coefficients obtained were found to be negatively significant for the oleic, palmitic and linoleic acid levels recorded. The cluster and principal component analyses performed revealed the importance of fatty acids and polyphenol contents in the clustering of olive ecotypes. The selection of olive ecotypes according to oil quality is possible, requiring further studies focusing especially on their agronomic characters.

Two validated, simple and precise densitometric high-performance thin-layer chromatography (HPTLC) quantification methods were proposed for both qualitative and quantitative estimation of oleuropein in Olea europaea leaves and a pharmaceutical product utilizing normal-phase and reversed-phase silica gel TLC plates. In method I, 10 × 20 cm glass plates coated with 0.2 mm thin layers of normal-phase silica gel 60 containing F254 (E-Merck, Germany) and a mixture of ethyl acetate‒methanol‒water (8:1:0.5, V/V) were used as the stationary and the mobile phase, respectively. Method II utilized 10 × 20 cm glass-backed plates supporting 0.2 mm layers of RP-18 silica gel 60 containing F254 (E-Merck, Germany) as the stationary phase and green solvents mixture composed of ethanol‒water (5.5:4.5, V/V) as the mobile phase. The two methods resulted in sharp, symmetrical, well-resolved peaks at RF values of 0.47 ± 0.02 and 0.78 ± 0.03 with linearity ranges 200‒1400 ng/spot (r2 = 0.9994) and 200‒1400 ng/spot (r2 = 0.9996) for method I and method II, respectively. Spots corresponding to oleuropein were scanned at 200 nm. The two methods complied with the ICH guidelines for validation. Due to simplicity, low cost and short analysis time, the methods can be good alternatives for the quality control of different products containing olive leaves extract or pure oleuropein.

Submitted 2020-07-01 | Accepted 2020-09-01 | Available 2020-12-01 https://doi.org/10.15414/afz.2020.23.mi-fpap.105-111 The by-products of olive oil production are not traditional feed ingredients in poultry nutrition. Despite their low nutritional value, they can be used not only as a source of energy and nutrients, but also as bioactive substances in nutrition of broiler chickens. The aim of the study was to determine whether the use of olive leaves and cake in feed mixtures for broilers affects their growth performance and bone mineralisation. In the feeding experiment 120 male Ross 308 broilers were used. At the age of 21 days the chickens were randomly assigned to 5 experimental groups each with two pens (replicates) of 12 animals and fed with 5 different feed mixtures without (Control) or supplemented with 5% or 10% olive leaves (OLeave5, OLeave10) or cake (OCake5, OCake10). Live weight and feed intake per pen were recorded weekly. At the end of the feeding experiment (42 days), 12 chickens per group were randomly selected and slaughtered. At the slaughter line, bone samples (femur, tibia and humerus) were taken from each chicken. The supplementation of olive leaves and cake had no significant effect on the growth performance and mineral content in femur, tibia and humerus of the broilers. The addition of 5 and 10% olive leaves to the feed resulted in higher Cu content in the humerus, but bone mineralisation did not change. In conclusion olive leaves and cake did not modify bone mineralisation but could be supplemented in the feed mixtures for broilers without negative effects on growth performance. Keywords: broiler, olive by-product, live weight, bone mineralisation References AL-HARTHI, M. (2017). The effect of olive cake, with or without enzymes supplementation, on growth performance, carcass characteristics, lymphoid organs and lipid metabolism of broiler chickens. 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Effects of different levels of two types of olive pulp with or without exogenous enzyme supplementation on broiler performance and economic parameters. Brazilian Journal of Poultry Science, 18(3), 489-500. http://dx.doi.org/10.1590/1806-9061-2015-0060 SUCHÝ, P. et al. (2009). Chemical composition of bone tissue in broiler chickens intended for slaughter. Czech Journal of Animal Science, 54(7), 324-330. https://doi.org/10.17221/1726-CJAS SURAI, P. F. (2014). Polyphenol compounds in the chicken/animal diet: from the past to the future. Journal of Animal Physiology and Animal Nutrition, 98(1), 19-31. https://doi.org/10.1111/jpn.12070 TALATY, P. N. et al. (2009). Life cycle changes in bone mineralization and bone size traits of commercial broilers. Poultry Science, 88(5), 1070-1077. https://doi.org/10.3382/ps.2008-00418 VARMAGHANY, et al. (2013). 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Leaves incorporation during the extraction of olive oils can enhance their chemical-sensory quality. Thus, leaves from cvs. Arbequina or Santulhana were added (1%, w/w) during the extraction of Arbequina oils using an Abencor system, being discussed the impacts on the phenolics and volatiles formation enzymatic pathways. Leaves addition contributed to a significant decrease (P-value, The authors are grateful to the Foundation for Science and Technology (FCT, Portugal) for financial support by national funds FCT/MCTES to CIMO (UIDB/00690/2020), CEB (UIDB/04469/2020), REQUIMTE-LAQV (UIDB/50006/2020) units and SusTEC (LA/P/0007/ 2020); to BioTecNorte operation (NORTE-01-0145-FEDER-000004), to Project “GreenHealth - Digital strategies in biological assets to improve well-being and promote green health” (Norte-01-0145-FEDER-000042) and to Project AgriFood XXI (NORTE-01-0145-FEDER-000041), funded by the European Regional Development Fund under the scope of Norte2020 - Programa Operacional Regional do Norte. Ítala M.G. Marx acknowledges the Ph.D. grant (SFRH/BD/137283/2018) provided by FCT. Nuno Rodrigues thanks to National funding by FCT- Foundation for Science and Technology, P.I., through the institutional scientific employment program-contract., info:eu-repo/semantics/publishedVersion

It has been noted that disease detection approaches based on deep learning are becoming increasingly important in artificial intelligence-based research in the field of agriculture. Studies conducted in this area are not at the level that is desirable due to the diversity of plant species and the regional characteristics of many of these species. Although numerous researchers have studied diseases on plant leaves, it is undeniable that timely diagnosis of diseases on olive leaves remains a difficult task. It is estimated that people have been cultivating olive trees for 6000 years, making it one of the most useful and profitable fruit trees in history. Symptoms that appear on infected leaves can vary from one plant to another or even between individual leaves on the same plant. Because olive groves are susceptible to a variety of pathogens, including bacterial blight, olive knot, Aculus olearius, and olive peacock spot, it has been difficult to develop an effective olive disease detection algorithm. For this reason, we developed a unique deep ensemble learning strategy that combines the convolutional neural network model with vision transformer model. The goal of this method is to detect and classify diseases that can affect olive leaves. In addition, binary and multiclassification systems based on deep convolutional models were used to categorize olive leaf disease. The results are encouraging and show how effectively CNN and vision transformer models can be used together. Our model outperformed the other models with an accuracy of about 96% for multiclass classification and 97% for binary classification, as shown by the experimental results reported in this study.

Olive mill wastewater (OMWW) is produced annually during olive oil extraction and contains most of the health-promoting 3-hydroxytyrosol of the olive fruit. To facilitate its recovery, enzymatic transesterification of hydroxytyrosol (HT) was directly performed in an aqueous system in the presence of ethyl acetate, yielding a 3-hydroxytyrosol acetate rich extract. For this, the promiscuous acyltransferase from Pyrobaculum calidifontis VA1 (PestE) was engineered by rational design. The best mutant for the acetylation of hydroxytyrosol (PestE_I208A_L209F_N288A) was immobilized on EziG

Hydroxytyrosol (HT), the main representative of polyphenols of olive oil, has been described as one of the most powerful natural antioxidants, also showing anti-inflammatory, antimicrobial, cardioprotective and anticancer activity in different type of cancers, but has been little studied in hematological neoplasms. The objective of this work was to evaluate the anticancer potential of HT in acute human leukemia T cells (Jurkat and HL60) and the anti-inflammatory potential in murine macrophages (Raw264.7). For this, cytotoxicity tests were performed for HT, showing IC

Olea welwitschii (Knobl.)Gilg & G.Schellenb. — Habit:Tree. Habitat:LMWF,LMDF;up to2 000 m.Distribution: II. Voucher: Lower Imenti Forest, Alt. 1 190 m, 26–28, Jun. 1974, Faden & Faden 74/901A (EA). Reference: Fries (1923b)., Published as part of Zhou, Ya-Dong, Mwachala, Geoffrey, Hu, Guang-Wan & Wang, Qing-Feng, 2022, Annotated checklist of the vascular plants of Mount Kenya, East Africa, pp. 1-108 in Phytotaxa 546 (1) on page 81, DOI: 10.11646/phytotaxa.546.1.1, http://zenodo.org/record/6550464, {"references":["Fries, T. C. E. (1923 b) Beitrage zur Kenntnis der Flora des Kenia, Mt. Aberdare und Mt. Elgon. III. Notizblatt des Koniglichen Botanischen Gartens und Museums zu Berlin 8 (78): 547 - 573. https: // doi. org / 10.2307 / 3994426"]}

Olea europaea L. subsp. cuspidata (Wall. & G.Don) Cif. — Habit: Small tree or tree. Habitat: LMWF, LMDF; 1 800–3 000 m. Distribution: II. Voucher: Near Lake Nkunga, Alt. 1 903 m, 8 Aug. 2015, SAJIT 003820 (HIB). References: Fries (1923b), Bussmann (1993, 1994, 2006), Bussmann & Beck (1995a, 1995b, 1998, 1999), Gathaara (1999)., Published as part of Zhou, Ya-Dong, Mwachala, Geoffrey, Hu, Guang-Wan & Wang, Qing-Feng, 2022, Annotated checklist of the vascular plants of Mount Kenya, East Africa, pp. 1-108 in Phytotaxa 546 (1) on page 80, DOI: 10.11646/phytotaxa.546.1.1, http://zenodo.org/record/6550464, {"references":["Fries, T. C. E. (1923 b) Beitrage zur Kenntnis der Flora des Kenia, Mt. Aberdare und Mt. Elgon. III. Notizblatt des Koniglichen Botanischen Gartens und Museums zu Berlin 8 (78): 547 - 573. https: // doi. org / 10.2307 / 3994426","Bussmann, R. W. (1993) The forest of Mt. Kenya (Kenya): A phytosociological approach with special reference to ecological problems. Ph. D. Thesis, Eberhard Karls Universitat Tubingen, Tubingen, 119 pp.","Bussmann, R. W. (1994) The forest of Mt. Kenya (Kenya): Vegetation, ecology, destruction and management of a tropical mountain forest ecosystem. Ph. D. dissertation, Universitat Bayreuth Startseite, Bayreuth, 252 pp.","Bussmann, R. W. (2006) Vegetation zonation and nomenclature of African Mountains: An overview. Lyonia 11 (1): 41 - 46.","Bussmann, R. W. & Beck, E. (1995 a) The forests of Mt. Kenya (Kenya), a phytosociological synopsis. Phytocoenologia 25 (4): 467 - 560. https: // doi. org / 10.1127 / phyto / 25 / 1995 / 467","Bussmann, R. W. & Beck, E. (1995 b) Regeneration and succession processes in the Cedar Forests (Juniperion procerae) of Mt. Kenya. Ecotropica 1: 79 - 84.","Bussmann, R. W. & Beck, E. (1998) The plant communities of the forests of Mount Kenya - First phytosociological and ecological data. In: Ojany, F. F. (Ed.) African mountains and highlands - planning for sustainable use of mountain resources. United Nations University, Tokyo, pp. 96 - 107.","Bussmann, R. W. & Beck, E. (1999) Vegetation units of Mt. Kenya Forest Reserve: Comment. In: Bitok (Ed.) Vegetations-okologosche, ethnobotanische und faunistische Beitrage uber Aquatorial-Afrika. Bayreuther Forum Okologie 64. Bayreuther Forum Okologie, Bayreuth, pp. 17 - 28.","Gathaara, G. N. (1999) Aerial survey of the destruction of Mt. Kenya, Imenti and Ngare Ndare forests reserves. Kenya Wildlife Service, Nairobi."]}

Olea capensis L. subsp. macrocarpa (C.H.Wright) I.Verd. — Habit: Tree. Habitat: LMWF, LMDF; 1 400–2 800 m. Distribution: II. Voucher: Chogoria Route, Alt. 2 355 m, 28 Jan. 2015, SAJIT 002946 (HIB). References: Fries (1923b), Bussmann (1993, 1994, 2006), Bussmann & Beck (1995a, 1998, 1999), K.F.S. (2010)., Published as part of Zhou, Ya-Dong, Mwachala, Geoffrey, Hu, Guang-Wan & Wang, Qing-Feng, 2022, Annotated checklist of the vascular plants of Mount Kenya, East Africa, pp. 1-108 in Phytotaxa 546 (1) on page 80, DOI: 10.11646/phytotaxa.546.1.1, http://zenodo.org/record/6550464, {"references":["Fries, T. C. E. (1923 b) Beitrage zur Kenntnis der Flora des Kenia, Mt. Aberdare und Mt. Elgon. III. Notizblatt des Koniglichen Botanischen Gartens und Museums zu Berlin 8 (78): 547 - 573. https: // doi. org / 10.2307 / 3994426","Bussmann, R. W. (1993) The forest of Mt. Kenya (Kenya): A phytosociological approach with special reference to ecological problems. Ph. D. Thesis, Eberhard Karls Universitat Tubingen, Tubingen, 119 pp.","Bussmann, R. W. (1994) The forest of Mt. Kenya (Kenya): Vegetation, ecology, destruction and management of a tropical mountain forest ecosystem. Ph. D. dissertation, Universitat Bayreuth Startseite, Bayreuth, 252 pp.","Bussmann, R. W. (2006) Vegetation zonation and nomenclature of African Mountains: An overview. Lyonia 11 (1): 41 - 46.","Bussmann, R. W. & Beck, E. (1995 a) The forests of Mt. Kenya (Kenya), a phytosociological synopsis. Phytocoenologia 25 (4): 467 - 560. https: // doi. org / 10.1127 / phyto / 25 / 1995 / 467","Bussmann, R. W. & Beck, E. (1998) The plant communities of the forests of Mount Kenya - First phytosociological and ecological data. In: Ojany, F. F. (Ed.) African mountains and highlands - planning for sustainable use of mountain resources. United Nations University, Tokyo, pp. 96 - 107.","Bussmann, R. W. & Beck, E. (1999) Vegetation units of Mt. Kenya Forest Reserve: Comment. In: Bitok (Ed.) Vegetations-okologosche, ethnobotanische und faunistische Beitrage uber Aquatorial-Afrika. Bayreuther Forum Okologie 64. Bayreuther Forum Okologie, Bayreuth, pp. 17 - 28."]}

Marking the tree canopies is an unavoidable step in any study working with high-resolution aerial images taken by a UAV in any fruit tree crop, such as olive trees, as the extraction of pixel features from these canopies is the first step to build the models whose predictions are compared with the ground truth obtained by measurements made with other types of sensors. Marking these canopies manually is an arduous and tedious process that is replaced by automatic methods that rarely work well for groves with a thick plant cover on the ground. This paper develops a standard method for the detection of olive tree canopies from high-resolution aerial images taken by a multispectral camera, regardless of the plant cover density between canopies. The method is based on the relative spatial information between canopies.The planting pattern used by the grower is computed and extrapolated using Delaunay triangulation in order to fuse this knowledge with that previously obtained from spectral information. It is shown that the minimisation of a certain function provides an optimal fit of the parameters that define the marking of the trees, yielding promising results of 77.5% recall and 70.9% precision.

The effectiveness of a Geographical Information Systems cost-distance tool for detecting landscape permeability in relation to the movement of pests in olive landscapes was established. The simplification of agricultural systems is linked to an increased incidence of pests on crops. Therefore, it is important to understand the impact of different land uses surrounding olive groves on pests. In this work, we analysed the effect of the structure of the olive landscape on the movement of two main olive pests—the olive fruit fly, Bactrocera oleae (Rossi) (Diptera: Tephritidae) and the olive moth, Prays oleae (Bernard) (Lepidopetera: Praydidae). We applied linear mixed effects models to analyse the relationship between pest abundance and cost-distance, using different hypotheses to evaluate those land uses that are favourable or unfavourable for the movement of these pests. The results show that this methodology is effective in detecting possible unfavourable land uses with a barrier effect, such as woodland and artificial land uses, and favourable land uses with a corridor effect such as olive groves. Whether other land uses, such as scrubland or riverbanks, act as a barrier or corridor depends on the pest and its life cycle stage. The effect that different land uses have in maintaining low levels of pest populations and ensuring the long-term sustainability of these agricultural systems are discussed. The implications of landscape permeability for the physical structure of the landscape and the dispersal of organisms, and the potential of that landscape to impact the continuous flow of natural processes are also addressed.

This study provides one of the earliest examples of fruit tree cultivation worldwide, demonstrating that olive (Olea europaea) and fig (Ficus carica) horticulture was practiced as early as 7000 years ago in the Central Jordan Valley, Israel. It is based on the anatomical identification of a charcoal assemblage recovered from the Chalcolithic (7200–6700 cal. BP) site of Tel Tsaf. Given the site’s location outside the wild olive’s natural habitat, the substantial presence of charred olive wood remains at the site constitutes a strong case for horticulture. Furthermore, the occurrence of young charred fig branches (most probably from pruning) may indicate that figs were cultivated too. One such branch was 14C dated, yielding an age of ca. 7000 cal. BP. We hypothesize that established horticulture contributed to more elaborate social contracts and institutions since olive oil, table olives, and dry figs were highly suitable for long-distance trade and taxation.

International audience; The circular economy and bioeconomy can contribute to transitioning towards more sustainable production and consumption in the olive oil sector. This article is the first to analyse multi-actor strategies and multi-level socioeconomic conditions for olive oil waste and by-product valorisation in the Mediterranean area using circular bioeconomy principles. Government policies, the strategies of corporations and farmers and consumers' perceptions are discussed, and various methods are applied, such as desk reviews, case studies and quantitative and qualitative surveys. The findings show strong aspirations for improved sustainability in the olive industry. Furthermore, waste and by-product valorisation strategies foster the creation of innovative practices. However, a common regulatory framework, public financial measures, new circular business models using innovative technologies, multi-actor collaboration and increased consumer awareness of the circular economy and new olive oil waste-based products are necessary for more efficient and sustainable use of olive resources. The policy and management recommendations presented in this study may aid in improving and innovating frameworks and practices for better sustainable management of valuable olive resources.

Nanomaterials in agriculture represent one of the most innovative method for protecting crops, due to possibility of being applied as nanopesticides or nanocarriers for active ingredients. Furthermore, nanotechnology could be combined with the concept of circular economy through the opportunity of obtaining highly technological nanometric materials starting from agro-industrial wastes. The present research evaluated the possibility of synthesizing cellulose nanocrystals (CNCs) from olive pruning wastes through chemical bleaching, reusing them as sustainable tool to control the causal agent of the olive knot disease (Pseudomonas savastanoi pv. savastanoi). CNCs showed an interesting in vitro effect in inhibiting bacterial growth and bacterial biofilm formation, as well as the ability of reducing bacterial epiphytic survival in a comparable way to copper sulphate on leaf surfaces, when used at 1% w/v. CNCs were at the same time investigated for their interaction with olive tree seedlings, showing no negative effects on leaf development, and a promising root uptake, indicating that CNCs could be used also as nanocarriers for active ingredients. Obtained results highlight the innovative possibility of designing sustainable plant protection strategies capable of revalorise lignocellulosic wastes, meaning a simultaneous low environmental impact thanks to reduction of traditional agrochemicals input.

Xylella fastidiosa is a xylem-limited bacterium causing a range of economically important plant diseases in hundreds of crops. Over the last decade, a severe threat due to Olive Quick Decline Syndrome (OQDS), caused by Xylella fastidiosa subspecies pauca, affected the Salento olive groves (Apulia, South-East Italy). Very few phyto-therapeutics, including a Zn/Cu citric acid biocomplex foliar treatment, were evaluated to mitigate this disease. However, the traditional foliar applications result in the agro-actives reaching only partially their target. Therefore the development of novel endo-therapeutic systems was suggested. Metabolite fingerprinting is a powerful method for monitoring both, disease progression and treatment effects on the plant metabolism, allowing biomarkers detection. We performed, for the first time, short-term monitoring of metabolic pathways reprogramming for infected Ogliarola salentina and Cima di Melfi olive trees after precision intravascular biocomplex delivery using a novel injection system. Upon endo therapy, we observed specific variations in the leaf content of some metabolites. In particular, the 1H NMR-based metabolomics approach showed, after the injection, a significant decrease of both the disease biomarker quinic acid and mannitol with simultaneous increase of polyphenols and oleuropein related compounds in the leaf’s extracts. This combined metabolomics/endo-therapeutic methodology provided useful information in the comprehension of plant physiology for future applications in OQDS control.

Genome-wide oil biosynthesis was explored by de novo sequencing two cultivated olive tree (Olea europaea) varieties (cv. Ayvalik and Picual). This is the first report of the former variety sequencing. As outgroups, raw reads of cv. Leccino and scaffold-level assembly of cv. Farga were also retrieved. Each of these four cultivars was chromosome-scale assembled into 23 pseudochromosomes, with 1.31 Gbp (Farga), 0.93 Gbp (Ayvalik), 0.7 Gbp (Picual), and 0.54 Gbp (Leccino) in size. Ab initio gene finding was performed on these assemblies, using wild olive tree (oleaster)-trained parameters. High numbers of gene models were predicted and anchored to the pseudochromosomes: 69,028 (Ayvalik), 55,073 (Picual), 63,785 (Farga), and 40,449 (Leccino). Using previously reported oil biosynthesis genes from wild olive tree genome project, the following homologous sequences were identified: 1,355 (Ayvalik), 1,269 (Farga), 812 (Leccino), and 774 (Picual). Of these, 358 sequences were commonly shared by all cultivars. Besides, some sequences were cultivar unique: Ayvalik (126), Farga (118), Leccino (46), and Picual (52). These putative sequences were assigned to various GO terms, ranging from lipid metabolism to stress tolerance, from signal transactions to development, and to many others, implicating that oil biosynthesis is synergistically regulated with involvement of various other pathways., This study was supported by the “Ministerio de Economía y Competitividad” (MINECO grant BIO2015-64737-R) and “Instituto Nacional de Investigación y Tecnología Agraria y Alimentaria” (MINECO and INIA RF 2012–00002-C2-02); “Consejería de Agricultura y Pesca” (041/C/2007, 75/C/2009 and 56/C/2010), “Consejería de Economía, Innovación y Ciencia” (P11-AGR-7322), and “Grupo PAI” (AGR-248) of “Junta de Andalucía”; and “Universidad de Córdoba” (“Ayuda a Grupos”), Spain.

During past few decades, endophytes have gained importance due their ability to produce bioactive compounds. Many medicinal plants are being exploited for the endophytic isolation to obtain drugs of interest. This study explored the fungal endophytes of Olive (Olea europaea L.) stem from which Fusarium redolens was selected for investigation of bioactive potential. The endophyte was identified using morphological characteristics and internal transcribed spacer ribosomal-deoxyribonucleic acid (ITS-rDNA) sequence analysis. The GCMS analysis of the crude extract yielded chrysophanol and fumaric acid. The culture filtrate of ethyl acetate extract showed significant cytotoxic potential against HepG2 cells, respectively. Furthermore, the screening of antioxidant potential of the ethyl acetate fungal extract using DPPH scavenging assay showed that Fusarium redolens extract exhibited potential activity with a significant EC50 value of 144.7 µg/mL.

The three-phase solvent system counter-current chromatography has been of great research interest, because it can separate compounds with a wide range of polarity. The solvent system of n-hexane/methyl tert-butyl ether/acetonitrile/water (5:5:7:5, v/v) was used for counter-current chromatographic comprehensive separation of olive leaves. The study adopted the normal elution mode. The middle phase and the lower phase (at a volume ratio of 7:3) were pumped into the column simultaneously, followed by eluting with the upper, middle, and lower phases in sequence. The retention rate of the stationary phase measured by the experiment was 73.5%. The upper phase was used to elute the nonpolar compounds, then the mobile phase was switched to the middle phase to elute the moderately hydrophobic compounds, finally, the polar compounds were eluted by the lower phase remaining in the chromatographic column. This method successfully separated eight compounds in one step within 270 min and five compounds were identified. The logP values of these five compounds were 7.44, 7.86, 4.16, -0.11, and 0.96, respectively, covering a wide range of polarities. The present study demonstrated that the three-phase solvent has a strong extraction capacity for ingredients from extremely hydrophilic compounds to extremely hydrophobic compounds.

Olive tree leaves are an abundant source of bioactive compounds with several beneficial effects for human health, including a protective role against many types of cancer. In this study, we investigated the effect of an extract, obtained from olive tree (Olea europaea L.) leaves (OLE), on proliferation, invasion, and epithelial to mesenchymal transition (EMT) on metastatic melanoma, the highly aggressive form of skin cancer and the deadliest diseases. Our results demonstrated that OLE inhibited melanoma cells proliferation through cell cycle arrest and induction of apoptotic cell death. Moreover, OLE suppressed the migration, invasion, and colonies formation of human melanoma cells. Similar to our in vitro findings, we demonstrated that the oral administration of OLE inhibited cutaneous tumor growth and lung metastasis formation in vivo by modulating the expression of EMT related factors. In addition, the anti-proliferative and anti-invasive effects of OLE against melanoma were also related to a simultaneous targeting of mitogen-activated protein kinase and PI3K pathways, both in vitro and in vivo. In conclusion, our findings suggest that OLE has the potential to inhibit the metastatic spread of melanoma cells thanks to its multifaceted mechanistic effects, and may represent a new add-on therapy for the management of metastatic melanoma.

Waste represents a cost for companies, in particular for agro-food companies, which can become a resource as a secondary material. In this work, we examine three products of olive-oil waste water, named MOMAST® (Plus30, PW25, and HY100). Based on the chemical composition, obtained with different methods, we hypothesized a possible application as food supplements in irritable bowel syndrome (IBS). We therefore studied MOMASTs on some targets linked to this pathology: antioxidant action and spontaneous and induced intestinal contractility of the ileum and colon. Plus30, which showed a more promising biological of activity also for its oleuropein content, was characterized by an interesting action against some microorganisms. The results highlighted the ability of Plus30 to modulate spontaneous and induced contractility, to exert a good antioxidant action, and to significantly act on various microorganisms. These effects are synergistic in the presence of antibiotics. In conclusion, we can confirm that Plus30 could be a great candidate as a food supplement in patients with IBS.

Despite increasing interest to investigate horizontal gene transfer as a leading cause of antibiotic resistance spread, the resistome is not only influenced by the influx and efflux of genes in different environments. Rather, the expression of existing genes under different stress conditions requires special attention. This study determined whether pre-adapting Lactiplantibacillus pentosus strains, isolated from Aloreña green table olives, to vegetable-based edible oils influence their phenotypic and genotypic responses to antibiotics. This has significant diet, food matrix, gut health, and food safety concerns. Pre-adapting L. pentosus strains to oils significantly changed their susceptibility profile to antibiotics. However, results generally differed among the three strains; although changes in the Minimum Inhibitory Concentration (MIC) of antibiotics occurred, it depended on the L. pentosus strain and the oil used for adaptation. The pre-adaptation of L. pentosus strains with olive, sunflower, argan and linseed oils induced gene expressions (e.g., rpsL, recA and uvrB) in several stress responses. Thus, to analyze this fact in-depth, transcriptional changes were reported in the selected potential probiotic L. pentosus CF2-10 adapted with olive or sunflower, rerouting its metabolic pathways to export toxic molecules through efflux pumps and ABC transporters. Pre-adaptation of some lactobacilli with olive or sunflower oils may represent a novel approach for manufacturing probiotic products with improved stability, functionality and robustness.

There is currently a worldwide consensus and recognition of the undoubted health benefits of the so-called Mediterranean diet, with its intake being associated with a lower risk of mortality. The most important characteristics of this type of diet are based on the consumption of significant amounts of fruit, vegetables, legumes, and nuts, which provide, in addition to some active ingredients, fiber and a proportion of vegetable protein, together with extra virgin olive oil (EVOO) as the main sources of vegetable fat. Fish and meat from poultry and other small farm animals are the main sources of protein. One of the main components, as already mentioned, is EVOO, which is rich in monounsaturated fatty acids and to a lesser extent in polyunsaturated fatty acids. The intake of this type of nutrient also provides an important set of phytochemicals whose health potential is widely spread and agreed upon. These phytochemicals include significant amounts of anthocyanins, stilbenes, flavonoids, phenolic acids, and terpenes of varying complexities. Therefore, the inclusion in the diet of this type of molecules, with a proven healthy effect, provides an unquestionable preventive and/or curative activity on an important group of pathologies related to cardiovascular, infectious, and cancerous diseases, as well as those related to the metabolic syndrome. The aim of this review is therefore to shed light on the nutraceutical role of two of the main phytochemicals present in

Alzheimer's disease (AD) is the most common form of dementia among several neurodegenerative disorders afflicting the elderly. AD is characterized by the deposition of extracellular amyloid-β (Aβ) plaques, disrupted blood-brain barrier (BBB), and neuroinflammation. Several studies have demonstrated the health benefits of olive oil and olive leaf extract (OLE) due to their polyphenolic content. The main phenolic compound in OLE is glycosylated oleuropein (OLG), while the aglycon form of oleuropein (OLA) exists in much lower amounts. This work aimed to evaluate the effect of a low dose of OLG-rich OLE and the mechanism(s) that contributed to the observed beneficial effects against Aβ pathology in the homozygous 5xFAD mouse model. Mice were fed with OLE-enriched diet (695 μg/kg body weight/day) for 3 months, starting at 3 months old. Overall findings demonstrated that OLE reduced neuroinflammation by inhibiting the NF-κB pathway and suppressing the activation of NLRP3 inflammasomes and RAGE/HMGB1 pathways. In addition, OLE reduced total Aβ brain levels due to increased clearance and reduced production of Aβ and enhanced BBB integrity and function, which collectively improved the memory function. Thus, the consumption of OLE as a dietary supplement is expected to stop and/or slow the progression of AD.

This study evaluates the comprehensive valorization of the byproducts derived from the two-phase olive oil elaboration process [i.e., olive washing water (OWW), olive oil washing water (OOWW), and olive mill solid waste (OMSW)] in a closed-loop process. Initially, the microalga

In this study, a successfully rapid, simple approach was applied for biosynthesis of silver nanoparticles AgNPs using for the first time the mixed leaves extract of Olea europaea subsp. europaea var. sylvestris and Pistacia lentiscus from natural association aimed to enhance their antimicrobial potential. The plant extract acts both as reducing and capping agents. When the aqueous extract was added to AgNO

The bioavailability and cycling of nutrients in soil are two of the most important factors for healthy plant growth and development in natural and agricultural ecosystems. Seasonal variations of some soil macronutrient (phosphorus and potassium) and micronutrient (copper, manganese, and zinc) contents were investigated in a natural olive (Olea europaea L.) grove (NO) and an agricultural olive gene garden (OGG) located in Adana, Turkey. Soils were sampled at 0-10 cm and at 10-20 cm depth in the months of November, February, May, and August between 2013 and 2015. Soil phosphorus, potassium, copper, manganese, and zinc contents were in the range between 0.37 and 8.65 mg kg

Cancer is one of the most widespread diseases globally and one of the leading causes of death. Known cancer treatments are chemotherapy, surgery, radiation therapy, targeted hormonal therapy, or a combination of these methods. Antitumor drugs, with different mechanisms, interfere with cancer growth by destroying cancer cells. However, anticancer drugs are dangerous, as they significantly affect both cancer cells and healthy cells. In addition, there may be the onset of systemic side effects perceived and mutagenicity, teratogenicity, and further carcinogenicity. Many polyphenolic extracts, taken on top of common anti-tumor drugs, can participate in the anti-proliferative effect of drugs and significantly reduce the side effects developed. This review aims to discuss the current scientific knowledge of the protective effects of polyphenols of the genera

Hepatotoma is the leading type of primary liver cancer in adults and third cause of death in the world. Hydroxytyrosol is a natural phenol existing in olive (Olea europaea L.). Hydroxytyrosol is the chief ingredient of olive oil, which was early deemed to be the most robust antioxidant in olive oil. Hydroxytyrosol is known to inhibit various types of cancer by different methods. This study was aimed to delineate the action of hydroxytyrosol on viability and [Ca