201. Clonally related but phenotypically divergent human cancer cell lines derived from a single follicular thyroid cancer recurrence (TT2609).
- Author
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Geldof AA, Versteegh LRT, van Mourik JC, Rooimans MA, Arwert F, Hermsen MA, Schadee-Eestermans IL, van Dongen GA, van der Valk P, van der Clement EHP, Lips P, and Teule GJ
- Subjects
- Animals, Cell Division, Female, Humans, Iodine pharmacokinetics, Karyotyping, Keratins metabolism, Male, Mice, Mice, Nude, Microscopy, Electron, Neoplasm Recurrence, Local genetics, Neoplasm Recurrence, Local pathology, Neoplasm Recurrence, Local physiopathology, Neoplasm Transplantation, Phenotype, Ploidies, Thyroglobulin metabolism, Thyroid Neoplasms genetics, Thyroid Neoplasms physiopathology, Transplantation, Heterologous, Tumor Stem Cell Assay, Tumor Suppressor Protein p53 metabolism, Thyroid Neoplasms pathology, Tumor Cells, Cultured pathology
- Abstract
Starting from different regional samples taken from a heterogeneous follicular thyroid cancer recurrence in a male patient, a series of cell cultures was initiated. Three stable cancer cell lines were successfully established (TT2609-A02, TT2609-B02, and TT2609-C02) and kept in continuous culture for more than 3 years. The lines are each characterized by a unique set of biological parameters such as morphology, ploidy state, cell proliferation rate, ultrastructure, thyroid marker expression, p53 expression, karyogram, agar clonogenic capacity and tumorigenicity as xenografts in nude mice. These characterization studies point to a marked heterogeneity at the level of the clinical tumor recurrence. Karyotype analysis of the cell lines showed a pattern of aberrations indicating that the lines are clonally related and that the A02 and C02 lines are subsequently derived from the more "original" tumor cell type B02 after a tetraploidization event. It is concluded that the obtained cell lines represent an in vitro/in vivo model for human follicular thyroid cancer. The availability of a series of cell lines for human follicular thyroid cancer, mimicking the biological heterogeneity observed in patient tumors, enables both detailed fundamental investigation of thyroid cancer cell biology and the experimental exploration of new treatment approaches.
- Published
- 2001
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