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312. A Pilot Study for the Neuroprotective Effect of Gongjin-dan on Transient Middle Cerebral Artery Occlusion-Induced Ischemic Rat Brain

Author

Sunwoo, Yun-Young, primary, Park, Sang In, additional, Chung, Yong-An, additional, Lee, Jisoo, additional, Park, Moon-Seo, additional, Jang, Kyung-Sool, additional, Maeng, Lee-So, additional, Jang, Dong-Kyu, additional, Im, Ruth, additional, Jung, Yu Jin, additional, Park, Soon A., additional, Kang, Eun-Sun, additional, Kim, Min-Wook, additional, and Han, Young-Min, additional

Published
2012
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314. Deletion of PRKAAtriggers mitochondrial fission by inhibiting the autophagy-dependent degradation of DNM1L

Author

Wang, Qilong, Wu, Shengnan, Zhu, Huaiping, Ding, Ye, Dai, Xiaoyan, Ouyang, Changhan, Han, Young-min, Xie, Zhonglin, and Zou, Ming-Hui

Abstract

ABSTRACTPRKAA (protein kinase, AMP-activated, α catalytic subunit) regulates mitochondrial biogenesis, function, and turnover. However, the molecular mechanisms by which PRKAA regulates mitochondrial dynamics remain poorly characterized. Here, we report that PRKAA regulated mitochondrial fission via the autophagy-dependent degradation of DNM1L (dynamin 1-like). Deletion of Prkaa1/AMPKα1 or Prkaa2/AMPKα2 resulted in defective autophagy, DNM1L accumulation, and aberrant mitochondrial fragmentation in the mouse aortic endothelium. Furthermore, autophagy inhibition by chloroquine treatment or ATG7small interfering RNA (siRNA) transfection, upregulated DNM1L expression and triggered DNM1L-mediated mitochondrial fragmentation. In contrast, autophagy activation by overexpression of ATG7 or chronic administration of rapamycin, the MTOR inhibitor, promoted DNM1L degradation and attenuated mitochondrial fragmentation in Prkaa2-deficient (prkaa2−/−) mice, suggesting that defective autophagy contributes to enhanced DNM1L expression and mitochondrial fragmentation. Additionally, the autophagic receptor protein SQSTM1/p62, which bound to DNM1L and led to its translocation into the autophagosome, was involved in DNM1L degradation by the autophagy-lysosome pathway. Gene silencing of SQSTM1markedly reduced the association between SQSTM1 and DNM1L, impaired the degradation of DNM1L, and enhanced mitochondrial fragmentation in PRKAA-deficient endothelial cells. Finally, the genetic (DNM1LsiRNA) or pharmacological (mdivi-1) inhibition of DNMA1L ablated mitochondrial fragmentation in the mouse aortic endothelium and prevented the acetylcholine-induced relaxation of isolated mouse aortas. This suggests that aberrant DNM1L is responsible for enhanced mitochondrial fragmentation and endothelial dysfunction in prkaaknockout mice. Overall, our results show that PRKAA deletion promoted mitochondrial fragmentation in vascular endothelial cells by inhibiting the autophagy-dependent degradation of DNM1L.

Published
2017
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