151. Impact of Polypyridyl Ru Complexes on Angiogenesis—Contribution to Their Antimetastatic Activity
- Author
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Ilona Gurgul, Olga Mazuryk, Kamila Stachyra, Rafał Olszanecki, Małgorzata Lekka, Michał Łomzik, Franck Suzenet, Philippe C. Gros, Małgorzata Brindell, Uniwersytet Jagielloński w Krakowie = Jagiellonian University (UJ), University of Lódź, Institut de Chimie Organique et Analytique (ICOA), Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Université d'Orléans (UO)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Institut de Chimie du CNRS (INC)-Centre National de la Recherche Scientifique (CNRS), Laboratoire Lorrain de Chimie Moléculaire (L2CM), Institut de Chimie du CNRS (INC)-Université de Lorraine (UL)-Centre National de la Recherche Scientifique (CNRS), This research was funded by the National Science Center in Poland for the OPUS Project No. 2016/21/B/NZ7/01081. I.G. acknowledges the support from InterDokMed project No. POWR.03.02.00-00-013/16. The APC was funded by the InterDokMed project No. POWR.03.02.00-00-013/16. F.S. acknowledges the support from Labex SynOrg (ANR-11-LABX-0029), Labex IRON (ANR-11-LABX-0018-01), the FEDER TECHSAB, the University of Orleans and the Centre-Val de Loire Region., and GROS, PHILIPPE C.
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[CHIM.THER] Chemical Sciences/Medicinal Chemistry ,[SDV.CAN]Life Sciences [q-bio]/Cancer ,[CHIM.THER]Chemical Sciences/Medicinal Chemistry ,Ligands ,migration ,Ruthenium ,Catalysis ,Inorganic Chemistry ,angiogenesis ,2,2'-Dipyridyl ,polypyridyl ruthenium (II) complexes ,[SDV.CAN] Life Sciences [q-bio]/Cancer ,Coordination Complexes ,Neoplasms ,cell elasticity ,Humans ,[CHIM.COOR]Chemical Sciences/Coordination chemistry ,Physical and Theoretical Chemistry ,endothelial cells ,cell adhesion properties ,cytotoxicity ,focal adhesions ,pseudovessel formation ,Molecular Biology ,Spectroscopy ,Organic Chemistry ,General Medicine ,[CHIM.COOR] Chemical Sciences/Coordination chemistry ,Computer Science Applications ,Phenanthrolines - Abstract
International audience; The use of polypyridyl Ru complexes to inhibit metastasis is a novel approach, and recent studies have shown promising results. We have reported recently that Ru (II) complexes gathering two 4,7-diphenyl-1,10-phenanthroline (dip) ligands and the one being 2,2′-bipyridine (bpy) or its derivative with a 4-[3-(2-nitro-1H-imidazol-1-yl)propyl (bpy-NitroIm) or 5-(4-{4′-methyl-[2,2′-bipyridine]-4-yl}but-1-yn-1-yl)pyridine-2-carbaldehyde semicarbazone (bpy-SC) moieties can alter the metastatic cascade, among others, by modulating cell adhesion properties. In this work, we show further studies of this group of complexes by evaluating their effect on HMEC-1 endothelial cells. While all the tested complexes significantly inhibited the endothelial cell migration, Ru-bpy additionally interrupted the pseudovessels formation. Functional changes in endothelial cells might arise from the impact of the studied compounds on cell elasticity and expression of proteins (vinculin and paxillin) involved in focal adhesions. Furthermore, molecular studies showed that complexes modulate the expression of cell adhesion molecules, which has been suggested to be one of the factors that mediate the activation of angiogenesis. Based on the performed studies, we can conclude that the investigated polypyridyl Ru (II) complexes can deregulate the functionality of endothelial cells which may lead to the inhibition of angiogenesis.
- Published
- 2022