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14,554 results on '"GAO Lei"'

304. Exceptionally high charge mobility in phthalocyanine-based poly(benzimidazobenzophenanthroline)-ladder-type two-dimensional conjugated polymers

Author

Wang, Mingchao, Fu, Shuai, Petkov, Petko, Fu, Yubin, Zhang, Zhitao, Liu, Yannan, Ma, Ji, Chen, Guangbo, Gali, Sai Manoj, Gao, Lei, Lu, Yang, Paasch, Silvia, Zhong, Haixia, Steinrück, Hans-Peter, Cánovas, Enrique, Brunner, Eike, Beljonne, David, Bonn, Mischa, Wang, Hai I., Dong, Renhao, and Feng, Xinliang

Published
2023
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316. Virome survey of the bat, Rhinolophus affinis, in Hainan Province, China

Author

Li, Zihan, Tang, Chuanning, Li, Youyou, Zhang, Yun, Wang, Gaoyu, Peng, Ruoyan, Huang, Yi, Hu, Xiaoyuan, Xin, Henan, Cao, Xuefang, Shen, Lingyu, Guo, Tonglei, He, Yijun, Fen, Boxuan, Huang, Juanjuan, Liang, Jian guo, Cui, Xiuji, Niu, Lina, Yang, Jian, Yang, Fan, Lu, Gang, Gao, Lei, Jin, Qi, Zhao, Mingming, Yin, Feifei, and Du, Jiang

Published
2024
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348. TRANS-ACTING SIRNA3-derived short interfering RNAs confer cleavage of mRNAs in rice

Author

Luo, Linlin, Yang, Xiaoyu, Guo, Mingxi, Lan, Ting, Yu, Yu, Mo, Beixin, Chen, Xuemei, Gao, Lei, and Liu, Lin

Subjects
Genetics, Biotechnology, Crops, Agricultural, Gene Expression Regulation, Plant, Genetic Variation, Genotype, MicroRNAs, Oryza, RNA Cleavage, RNA, Plant, RNA, Small Interfering, Biological Sciences, Agricultural and Veterinary Sciences, Plant Biology & Botany
Abstract

Plant TRANS-ACTING SIRNA3 (TAS3)-derived short interfering RNAs (siRNAs) include tasiR-AUXIN RESPONSE FACTORs (ARFs), which are functionally conserved in targeting ARF genes, and a set of non-tasiR-ARF siRNAs, which have rarely been studied. In this study, TAS3 siRNAs were systematically characterized in rice (Oryza sativa). Small RNA sequencing results showed that an overwhelming majority of TAS3 siRNAs belong to the non-tasiR-ARF group, while tasiR-ARFs occupy a diminutive fraction. Phylogenetic analysis of TAS3 genes across dicot and monocot plants revealed that the siRNA-generating regions were highly conserved in grass species, especially in the Oryzoideae. Target genes were identified for not only tasiR-ARFs but also non-tasiR-ARF siRNAs by analyzing rice Parallel Analysis of RNA Ends datasets, and some of these siRNA-target interactions were experimentally confirmed using tas3 mutants generated by genome editing. Consistent with the de-repression of target genes, phenotypic alterations were observed for mutants in three TAS3 loci in comparison to wild-type rice. The regulatory role of ribosomes in the TAS3 siRNA-target interactions was further revealed by the fact that TAS3 siRNA-mediated target cleavage, in particular tasiR-ARFs targeting ARF2/3/14/15, occurred extensively in rice polysome samples. Altogether, our study sheds light into TAS3 genes in plants and expands our knowledge about rice TAS3 siRNA-target interactions.

Published
2022

349. Arabidopsis RBV is a conserved WD40 repeat protein that promotes microRNA biogenesis and ARGONAUTE1 loading

Author

Liang, Chao, Cai, Qiang, Wang, Fei, Li, Shaofang, You, Chenjiang, Xu, Chi, Gao, Lei, Cao, Dechang, Lan, Ting, Zhang, Bailong, Mo, Beixin, and Chen, Xuemei

Subjects
Biological Sciences, Bioinformatics and Computational Biology, Human Genome, Genetics, Biotechnology, Arabidopsis, Arabidopsis Proteins, Argonaute Proteins, MicroRNAs, Nuclear Proteins, WD40 Repeats
Abstract

MicroRNAs (miRNAs) play crucial roles in gene expression regulation through RNA cleavage or translation repression. Here, we report the identification of an evolutionarily conserved WD40 domain protein as a player in miRNA biogenesis in Arabidopsis thaliana. A mutation in the REDUCTION IN BLEACHED VEIN AREA (RBV) gene encoding a WD40 domain protein led to the suppression of leaf bleaching caused by an artificial miRNA; the mutation also led to a global reduction in the accumulation of endogenous miRNAs. The nuclear protein RBV promotes the transcription of MIR genes into pri-miRNAs by enhancing the occupancy of RNA polymerase II (Pol II) at MIR gene promoters. RBV also promotes the loading of miRNAs into AGO1. In addition, RNA-seq revealed a global splicing defect in the mutant. Thus, this evolutionarily conserved, nuclear WD40 domain protein acts in miRNA biogenesis and RNA splicing.

Published
2022

350. Enhanced Gradient for Differentiable Architecture Search

Author

Zhang, Haichao, Hao, Kuangrong, Gao, Lei, Tang, Xuesong, and Wei, Bing

Subjects
Computer Science - Computer Vision and Pattern Recognition
Abstract

In recent years, neural architecture search (NAS) methods have been proposed for the automatic generation of task-oriented network architecture in image classification. However, the architectures obtained by existing NAS approaches are optimized only for classification performance and do not adapt to devices with limited computational resources. To address this challenge, we propose a neural network architecture search algorithm aiming to simultaneously improve network performance (e.g., classification accuracy) and reduce network complexity. The proposed framework automatically builds the network architecture at two stages: block-level search and network-level search. At the stage of block-level search, a relaxation method based on the gradient is proposed, using an enhanced gradient to design high-performance and low-complexity blocks. At the stage of network-level search, we apply an evolutionary multi-objective algorithm to complete the automatic design from blocks to the target network. The experiment results demonstrate that our method outperforms all evaluated hand-crafted networks in image classification, with an error rate of on CIFAR10 and an error rate of on CIFAR100, both at network parameter size less than one megabit. Moreover, compared with other neural architecture search methods, our method offers a tremendous reduction in designed network architecture parameters.

Published
2021

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