301. CD8+ T cell-mediated suppression of autoimmunity in a murine lupus model of peptide-induced immune tolerance depends on Foxp3 expression.
- Author
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Singh RP, La Cava A, Wong M, Ebling F, and Hahn BH
- Subjects
- Animals, Antibodies, Antinuclear biosynthesis, CD4-Positive T-Lymphocytes immunology, CD8-Positive T-Lymphocytes drug effects, Disease Models, Animal, Female, Forkhead Transcription Factors antagonists & inhibitors, Forkhead Transcription Factors genetics, Immunoglobulin G biosynthesis, Mice, Mice, Inbred Strains, Peptides immunology, RNA, Messenger analysis, RNA, Messenger metabolism, T-Lymphocytes, Helper-Inducer immunology, Transforming Growth Factor beta genetics, Transforming Growth Factor beta metabolism, Transforming Growth Factor beta pharmacology, Autoimmunity, CD8-Positive T-Lymphocytes immunology, Forkhead Transcription Factors metabolism, Immune Tolerance, Lupus Erythematosus, Systemic immunology
- Abstract
Systemic lupus erythematosus is an autoimmune disease caused by autoantibodies, including IgG anti-DNA. New Zealand Black/New Zealand White F(1) female mice, a model of spontaneous polygenic systemic lupus erythematosus, tolerized with an artificial peptide (pConsensus) based on anti-DNA IgG sequences containing MHC class I and class II T cell determinants, develop regulatory CD4+CD25+ T cells and CD8+ inhibitory T cells (CD8+ Ti), both of which suppress autoantibody production. CD8+ Ti inhibit primarily via secretion of TGF-beta. In the present study, we show that the inhibitory function of CD8+ T cells from tolerized mice is sustained for up to 8 wk and at all times depends on expression of Foxp3. Both CD28-positive and CD28-negative CD8+ T cells contain inhibitory cells, but the expression of mRNA for Foxp3 and for TGF-beta is higher and lasts longer in the CD28- subset. In vitro addition of TGF-beta (in the presence of IL-2) induces Foxp3 expression in a dose-response manner. Gene inhibition or blockade with small interfering RNA of Foxp3 abrogates the ability of the CD8+ Ti to inhibit anti-DNA production and the proliferation of CD4+ Th cells. Moreover, a significant correlation between expression of Foxp3 and ability of CD8+ Ti to secrete TGF-beta is observed. Therefore, CD8+ Ti in this system of tolerance are similar to CD4+CD25+ regulatory T cells in their dependence on expression of Foxp3, and there may be a bidirectional Foxp3/TGF-beta autocrine loop that determines the ability of the CD8+ T cells to control autoimmunity.
- Published
- 2007
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