251. Effect of bisphosphonates and gallium on dentin resorption in vitro.
- Author
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Liewehr FR, Craft DW, Primack PD, Kulild JC, Turgeon DK, Sutherland DE, Schuster GS, and Pashley DH
- Subjects
- Animals, Cell Adhesion drug effects, Chick Embryo, Dentin metabolism, Diphosphates therapeutic use, Gallium therapeutic use, Humans, In Vitro Techniques, Molar, Third, Osteoclasts drug effects, Tooth Avulsion, Tooth Replantation, Dentin drug effects, Diphosphates pharmacology, Gallium pharmacology, Root Resorption prevention & control
- Abstract
Replacement resorption may follow the replantation of an avulsed tooth. Currently there is no effective treatment for replacement resorption. The purpose of this study was to investigate the effect of bisphosphonates and gallium nitrate, which have been shown to reduce bone resorption, on cells which resorb dentin. Osteoclast-like cells were obtained by culturing cells from prenatal chick tibeas. These cells were seeded onto slices of human dentin which had been soaked in either saline (control), or solutions of 10(-5) M 1-hydroxyethylidene-1, 1-bisphosphonic acid (EHBP), 10(-6) M dichloromethylene bisphosphonic acid (Cl2MBP), or 10(-6) M gallium nitrate. Resorption was measured by counting the number of resorptive lacunae produced by the cells. Results indicated that the experimental groups did not differ significantly from each other, but each exhibited significantly reduced resorption compared with saline controls (p < 0.01). These results suggested that the experimental treatment reduced dentinal resorption by the osteoclast-like cells, and that these agents might be useful to prevent or at least postpone replacement resorption in avulsed teeth.
- Published
- 1995
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