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ABSTRAK: Penelitian ini bertujuan untuk mengangkat masalah mengenai penggunaan media kado ilmu dalam meningkatkan kemampuan menulis cerpen pada peserta didik. Penelitian ini merupakan salah satu jenis penelitian tindakan Kelas (PTK). Penelitian ini menggunakan metode deskriptif kualitataif yaitu pengumpulan data berupa data yang ditemukan pada saat melakukan penelitian dengan menggunakan media kadoilmu pada pesertadidik. Teknik yang dipakai dalam penelitian ini yaitu teknik non tes berupa observasi (pengamatan) terhadap peserta didik dan pendidik, dan teknik tes berupa hasil pekerjaan peserta didik yang di lakukan untuk mengetahui presentase ketuntasan hasilbelajar peserta didik. Teknik analisis data yaitu data hasil pekerjaan peserta didik yang dianalisis dengan cara membaca, memeriksa, dan menilai. Sehingga berdasarkan hasil penelitian tersebut disimpulkan bahwa pembelajaran dengan menggunakan media kado ilmu dapat meningkatkan kemampuan peserta didik dalam menuli scerpen, yakni dengan adanya ketuntasan belajar peserta didik pada siklus 1 yang tuntas 8 orang atau 28,57%, yang tidak tuntas 20 orang atau 71,43% sedangkan pada siklus II jumlahpeserta didik yang tuntasmeningkat menjadi 27 orang atau 96,42%, dengan 1 orang atau 4,00% yang menunjukan tidak tuntas. KATA KUNCI: Media Kadoilmu,Cerpen,Pesertadidik. >� THE USE OF SCIENTIFIC MEDIA MEDIA IN IMPROVING THE WRITING ABILITY OF STUDENTS IN EDUCATION� ABSTRACT: This study aims to raise the issue of the use of science gift media in improving short story writing skills in students. This research is one type of Classroom Action Research (CAR). This research uses descriptive qualitative method that is data collection in the form of data found at the time of conducting research using gift items on students. The techniques used in this study are non-test techniques in the form of observations (observers) of students and educators, and test techniques in the form of student work done to determine the percentage of completeness of student learning outcomes. Data analysis technique is the data of students' work which is analyzed by reading, checking, and assessing. So based on the results of the study it was concluded that learning by using science gift media can improve the ability of students in writing scerpenes, namely by completing the learning of students in cycle 1 which is complete 8 people or 28.57%, which is not completed 20 people or 71, 43% while in the second cycle the number of students who completed completed increased to 27 people or 96.42%, with 1 person or 4.00% who showed incomplete. KEYWORDS: Kadoilmu Media, Short Stories, Islamic Education Participants

The development of the shipbuilding industry is expected to meet the needs of the Indonesian Navy and the commercial vessels, and to support Indonesia's marine policy. The purpose of this study is to see the Shipbuilding industry Competitiveness, the influence of Technology Transfer to the Shipbuilding industry Competitiveness, and the influence of the industrial clusters on the Shipbuilding industry Competitiveness, as well as to analyze the institutional model of the Shipbuilding industry Competitiveness. This study uses the descriptive analysis, the Structural Equation Modeling (SEM) for the model causality testing, and the Interpretative Structural Modeling (ISM) for the institutional model of the competitiveness of the Shipbuilding Industry. This study uses the primary data, namely a survey of defense industry players, the national industry, the defense equipment users, the government institutions, the research institutes, and the universities that are determined purposively. ISM data are obtained from questionnaires and Forum Group Discussion (FGD) with 13 speakers representing academia, industry, and government. The results of the analysis of SEM state that the indicators on the industrial clusters, the competitiveness, and the technology transfer have a significant and real contribution to these variables. This research also shows that the industrial clusters and the technology transfer have a direct and significant effect on the competitiveness and the industrial clusters directly and significantly affect the technology transfer. However, the industrial clusters also have an indirect effect on competitiveness through the technology transfer to the shipbuilding industry. The results of the analysis of ISM conclude that the stakeholders involved have the greatest driving force, namely the Ministry of Defense and Ministry of State-Owned Enterprises, while the important factor affected by the stakeholders in strengthening the competitiveness of the shipbuilding industry is the Indonesian Navy Headquarters.

Abstract Background Ethanol biorefineries need to lower their overall production costs to become economically feasible. Two strategies to achieve this are to reduce costs using cheaper feedstocks or to increase the ethanol production yield. Low-cost feedstocks usually have high non-structural components (NSC) content; therefore, a new process is necessary to accommodate these feedstocks and overcome the negative effects of NSC. This study developed a novel ethanol biorefinery process including a biomass preprocessing step that enabled the use of lower-cost feedstocks while improving ethanol production without detoxification (overliming). Two types of poplar feedstocks were used, low-quality whole-tree chips (WTC) and high-quality clean pulp chips (CPC), to determine if the proposed process is effective while using feedstocks with different NSC contents. Results Technical assessment showed that acidic preprocessing increased the monomeric sugar recovery of WTC from 73.2% (untreated) to 87.5% due to reduced buffering capacity of poplar, improved sugar solubilization during pretreatment, and better enzymatic hydrolysis conversion. Preprocessing alone significantly improved the fermentability of the liquid fraction from 1–2% to 49–56% for both feedstocks while overliming improved it to 45%. Consequently, it was proposed that preprocessing can substitute for the detoxification step. The economic assessment revealed that using poplar WTC via the new process increased annual ethanol production of 10.5 million liters when compared to using CPC via overliming (base case scenario). Also, savings in total operating costs were about $10 million per year when using cheaper poplar WTC instead of CPC, and using recycled water for preprocessing lowered its total operating costs by 45-fold. Conclusions The novel process developed in this study was successful in increasing ethanol production while decreasing overall costs, thus facilitating the feasibility of lignocellulosic ethanol biorefineries. Key factors to achieving this outcome included substituting overliming by preprocessing, enabling the use of lower-quality feedstock, increasing monomeric sugar recovery and ethanol fermentation yield, and using recycled water for preprocessing. In addition, preprocessing enabled the implementation of an evaporator-combustor downstream design, resulting in a low-loading waste stream that can be treated in a wastewater treatment plant with a simple configuration.

The modern theory of argumentation is an interdisciplinary field of research. The sphere of interest of argumentation theory embraces the most effective tools of persuasion in interpersonal communication. At the same time, the focus is on studying not only how the argumentative process is built but also the areas where argumentation is used. Such interest contributes in particular to the study of cultural features, which in turn influence the development of the argumentative process. A key feature of this process is multimodality, which consists of the use of verbal and nonverbal modes. This paper is devoted to the analysis of our research project focused on the multimodal view on aggressiveness in interpersonal arguing.

The fighter aircraft project is one of the defense and security equipment (alpalhankam or alat peralatan pertahanan dan keamanan) priority programs contained in the defense industry roadmap. Indonesia and South Korea undertook a joint development program in developing 4.5 generation fighter aircraft. However, Indonesia experiences several obstacles in the development of fighter aircraft, one of which is the ability of disparity and mastery of fighter technology. One of the critical components used as one of the development programs is simulator technology development. Mastery of simulator technology can improve the ability of Technology Readiness Level (TRL) from technology development fighter aircraft and can cause multiplier effects in other fields. Therefore we need a development strategy that is appropriate for the development of simulator technology. This study aims to analyze and define the benchmark level of performance or from the critical components which will then be used as a reference for the development strategy of the 4.5 generation fighter simulator for joint development projects. The method used in this study is a mixed-method with an approach system engineering uses quality function deployment tools and Critical Technology Element (CTE) technology assessment to identify which CTE then used as a reference strategy for the development of fighter simulator technology. The results of the study show that there are several CTE values along with simulator technology performance scores, among others mission 92.5%, data 83.56%, motion 52.51%, and strategic options that can be used in the technological development of fighter simulator generation 4.5 such as strategic joint development, self-development, and local content/offset.

For every national minority cultivating traditions and customs is an important factor of identity. The same applies to the Lusatian minority, for which customs are expressions unifying and deepening their national awareness as well as playing an important part in their way of life. Among the Lusatian customs, a special place is occupied by traditions related to Easter. These include decorating Easter eggs, Easter singing, Holy Thursday presents for children, fetching Easter water, rolling Easter eggs, Easter Fire or the Easter Riders on Easter Sunday.

Argumentation theory is a crucial discipline for the development of the modern world. It provides innovative ways of evolution in interdisciplinary studies. Multimodality, which is the subject of this research, is an essential feature of human nature. Comparative analysis of modern views on multimodal argumentation and their generalization allows interpreting multimodal argumentation as “a social activity in which debaters use various modes to promote a particular point of view and study its acceptability.” The structural elements of multimodal argumentation are verbal, visual, sound, taste, etc. modes. The involvement of the latter an increases the efficiency of argumentation.

Introduction and objective. Depression is a disorder that medical students are subjected to. The aim of the research was to investigate the occurrence of subjective feeling of depressive symptoms among Polish medical students during the course of medical studies and to determine predictors of this phenomenon that are associated with studies and lifestyle. Material and method. The research tool was author’s questionnaire. 1023 medical students from each year of medical education and different Polish universities took part in the study. Results. 52.79% of the respondents had noticed symptoms that might had pointed to depression during the course of their medical studies. The research suggests that predictors of subjective feeling of depressive symptoms among medical students are: repeating a university subject, repeating a year in university, low self-assessment of academic performance during studying, considering dropping out of university, low satisfaction from the choice of studying medicine, reaching for alcohol in order to de-stress or discharge negative emotions, not having interests that enable to relax, not doing sports regularly, not participating in social gatherings often enough, not sleeping enough, having problems with maintaining stable body weight and not being religious. Conclusions. There are many lifestyle and studies connected factors associated with depressive symptoms among Polish medical students. The study suggests that it is important for students and their communities to pay attention to the emergence of them in order to improve the well-being of students.

Introduction and objective. Depression is a disease that affects over 264 million people worldwide. According to research, the problem of depression concerns dentistry students. The aim of the research was to investigate the prevalence and severity of depressive symptoms among Polish dental students, determine factors associated with this phenomenon and examine the relationship between these factors and depressive symptoms. Material and method. The participants of the study were dental students enrolled in each of the 10 Polish universities that offer dental education. The research tool was an author’s questionnaire and a Polish-language version of 9-Item-Patient Health Questionnaire (PHQ-9). Results. A total of 247 dental students took part in the study. The statistical analysis showed that 53.85% of the respondents were provisionally diagnosed with major depression. 8.91% of the participants had symptoms of severe depression. The research suggests that not doing sports regularly and not getting enough sleep are factors associated with depression among Polish dental students. Conclusions. Depressive symptoms among Polish dental students are common. Results suggest that it is important for students to maintain physical activity and take care of proper sleep hygiene. Further research needs to be done in order to explore more factors associated with depressive symptoms in the above-mentioned group.

Abstract Background Similar to biofuels, numerous chemicals produced from petroleum resources can also be made from biomass. In this research we investigate cradle to biorefinery exit gate life cycle impacts of producing acetic acid from poplar biomass using a bioconversion process. A key step in developing acetic acid for commercial markets is producing a product with 99.8% purity. This process has been shown to be potentially energy intensive and in this work two distillation and liquid–liquid extraction methods are evaluated to produce glacial bio-acetic acid. Method one uses ethyl acetate for extraction. Method two uses alamine and diisobutyl ketone. Additionally two different options for meeting energy demands at the biorefinery are modeled. Option one involves burning lignin and natural gas onsite to meet heat/steam and electricity demands. Option two uses only natural gas onsite to meet heat/steam demands, purchases electricity from the grid to meet biorefinery needs, and sells lignin from the poplar biomass as a co-product to a coal burning power plant to be co-fired with coal. System expansion is used to account for by-products and co-products for the main life cycle assessment. Allocation assessments are also performed to compare the life cycle tradeoffs of using system expansion, mass allocation, or economic allocation for bio-acetic acid production. Finally, a sensitivity analysis is conducted to determine potential effects of a decrease in the fermentation of glucose to acetic acid. Results Global warming potential (GWP) and fossil fuel use (FFU) for ethyl acetate extraction range from 1000–2500 kg CO2 eq. and 32–56 GJ per tonne of acetic acid, respectively. Alamine and diisobutyl ketone extraction method GWP and FFU ranges from −370–180 kg CO2 eq. and 15−25 GJ per tonne of acetic acid, respectively. Conclusions Overall the alamine/diisobutyl ketone extraction method results in lower GWP and FFU values compared to the ethyl acetate extraction method. Only the alamine/diisobutyl extraction method finds GWP and FFU values lower than those of petroleum based acetic acid. For both extraction methods, exporting lignin as a co-product produced larger GWPs and FFU values compared to burning the lignin at the biorefinery.

Abstract Purpose In living organisms, the positron-electron annihilation (occurring during the PET imaging) proceeds in about 30% via creation of a metastable ortho-positronium atom. In the tissue, due to the pick-off and conversion processes, over 98% of ortho-positronia annihilate into two 511 keV photons. In this article, we assess the feasibility for reconstruction of the mean ortho-positronium lifetime image based on annihilations into two photons. The main objectives of this work include the (i) estimation of the sensitivity of the total-body PET scanners for the ortho-positronium mean lifetime imaging using 2γ annihilations and (ii) estimation of the spatial and time resolution of the ortho-positronium image as a function of the coincidence resolving time (CRT) of the scanner. Methods Simulations are conducted assuming that radiopharmaceutical is labeled with 44 S c isotope emitting one positron and one prompt gamma. The image is reconstructed on the basis of triple coincidence events. The ortho-positronium lifetime spectrum is determined for each voxel of the image. Calculations were performed for cases of total-body detectors build of (i) LYSO scintillators as used in the EXPLORER PET and (ii) plastic scintillators as anticipated for the cost-effective total-body J-PET scanner. To assess the spatial and time resolution, the four cases were considered assuming that CRT is equal to 500 ps, 140 ps, 50 ps, and 10 ps. Results The estimated total-body PET sensitivity for the registration and selection of image forming triple coincidences (2γ+γ prompt) is larger by a factor of 13.5 (for LYSO PET) and by factor of 5.2 (for plastic PET) with respect to the sensitivity for the standard 2γ imaging by LYSO PET scanners with AFOV = 20 cm. The spatial resolution of the ortho-positronium image is comparable with the resolution achievable when using TOF-FBP algorithms already for CRT = 50 ps. For the 20-min scan, the resolution better than 20 ps is expected for the mean ortho-positronium lifetime image determination. Conclusions Ortho-positronium mean lifetime imaging based on the annihilations into two photons and prompt gamma is shown to be feasible with the advent of the high sensitivity total-body PET systems and time resolution of the order of tens of picoseconds.

Abstract Background Lung atelectasis are nonventilated parts of lung tissue and occur as a result of the collapse of the pulmonary parenchyma (alveoli). Various therapeutic procedures for inflating the collapsed pulmonary parenchyma, such as bronchial aspiration and/or standard recruitment maneuvers, are not always successful. Case presentation We report a case of a 23-year-old Croatian man with a parapharyngeal abscess on the left side of the neck with spreading of infection in the mediastinum and left side of the thorax and consequent major atelectasis of the left lung. The patient was mechanically ventilated. We decided to apply a new method in which a pulmonary artery catheter was placed (guided by bronchoscope) on the entrance to the lower left bronchus. The pulmonary artery catheter balloon was inflated to achieve bronchial closure. Using another respirator, we ventilated the affected lobe separately with continuously high pressure of 30 cmH2O. After 30 minutes, we removed the pulmonary artery catheter from the lower left bronchus and placed it in the upper left bronchus and repeated the procedure. Our method allowed a significantly longer duration (30 minutes) of continuously high pressure of 30 cmH2O separately to only one of the total of five lobes of the lungs while the other four lobes were simultaneously ventilated continuously with protective ventilation mode. Conclusion Use of a pulmonary artery catheter and two respirators in our patient’s case proved to be a successful method for recruiting the atelectatic lung while maintaining protective ventilation of the lung segments without atelectasis.

Introduction and objective. Depression is a disease that impairs daily functioning. According to research, as much as 27.2% of medical students suffer from depression or show depressive symptoms and 11.1% of them experience suicidal ideation. The aim of the research was to investigate the occurrence of subjective feeling of depressive symptoms among Polish medical students during the course of their medical studies and to determine predictors of this phenomenon that are related to student’s environment. Material and method. A total of 1023 medical students from each year of medical education and different Polish universities took part in the study. The research tool was an author’s questionnaire. Obtained data was subjected to a statistical analysis. To assess the relationship between two variables, the chi-square test of independence was used. The level of significance was set at p < 0.05. Results. The statistical analysis showed that most respondents (52.79%) had noticed symptoms that might had pointed to depression during their medical studies. A statistically significant link was found between subjective feeling of depressive symptoms during the course of medical studies and: mother’s level of education, relationship with parents, relationship with siblings, relationship with friends, knowing a person that one could count on in a difficult situation, knowing a person in which one could trust with all their problems, frequent feelings of loneliness and feeling that one doesn’t participate in social gatherings often enough. Conclusions. The prevalence of subjective feeling of depressive symptoms among medical students is high. Results suggest that good relationships with others are important in prevention of depression.

To avoid detection from Radio Detection and Ranging (Radar), one of the efforts is to use Radar absorbing material. One of the Radar wave absorbing materials is Perovskite CoTiO3. This Paper investigated the ability of CoTi(1-x)Mn(x)O3 to absorb the Radar wave. CoTi(1-x)Mn(x)O3 with variations x = 0, 0.01, 0.02, and 0.03 have been successfully synthesized using the mechanical milling method. The XRD pattern shows that the sample formed was single phase CoTiO3. Surface morphology resulting from measurements with SEM shows homogeneous particles and an average size of 200 nm. The results of measurements with VNA at X-band frequency (8.20 GHz - 12.4 GHz) show that the absorption ability of electromagnetic waves from CoTiO3 increases with the increase in doping from Mn4+. Maximum results obtained at the composition x=0.03 (CoTi0.97Mn0.03O3) with a reflection loss (RL) value is -14.56 dB (%Abs is 81.3%) at a frequency of 9.96 GHz. This result proves that CoTi(1-x)Mn(x)O3 can be used as a Radar absorbing material at X-band frequency.

BackgroundPathological response to neoadjuvant treatment for patients with high-grade serous ovarian carcinoma (HGSOC) is assessed using the chemotherapy response score (CRS) for omental tumor deposits. The main limitation of CRS is that it requires surgical sampling after initial neoadjuvant chemotherapy (NACT) treatment. Earlier and non-invasive response predictors could improve patient stratification. We developed computed tomography (CT) radiomic measures to predict neoadjuvant response before NACT using CRS as a gold standard.MethodsOmental CT-based radiomics models, yielding a simplified fully interpretable radiomic signature, were developed using Elastic Net logistic regression and compared to predictions based on omental tumor volume alone. Models were developed on a single institution cohort of neoadjuvant-treated HGSOC (n = 61; 41% complete response to NCT) and tested on an external test cohort (n = 48; 21% complete response).ResultsThe performance of the comprehensive radiomics models and the fully interpretable radiomics model was significantly higher than volume-based predictions of response in both the discovery and external test sets when assessed using G-mean (geometric mean of sensitivity and specificity) and NPV, indicating high generalizability and reliability in identifying non-responders when using radiomics. The performance of a fully interpretable model was similar to that of comprehensive radiomics models.ConclusionsCT-based radiomics allows for predicting response to NACT in a timely manner and without the need for abdominal surgery. Adding pre-NACT radiomics to volumetry improved model performance for predictions of response to NACT in HGSOC and was robust to external testing. A radiomic signature based on five robust predictive features provides improved clinical interpretability and may thus facilitate clinical acceptance and application.

Osteoarthritis Knee (OA) is the leading cause of pain and disability. This may affect the patient's quality of life (QoL) and lead to the onset of mental disorders. The aim of this study was 1) To find the correlation between the severity of OA, depression, and QoL. 2) To compare the severity of OA knee and QoL between urban and rural areas. 199 patients were diagnosed with OA. All patients had self-assessment with questionnaires in terms of 1) demographic data, 2) the knee severity by using Oxford Knee Score, 3) Depression screening by using Patient Health Questionnaire, and 4) World Health Organization Quality of Life Brief-Thai. The results revealed that OA knee patients had excellent (no abnormal symptoms) and good (mild symptoms) levels of severity were 34.2% and 32.2%, respectively. They also had a good level of QoL. The correlation between residential area and other variables were age group (p \< 0.01), severity of osteoarthritis (p \< 0.01), and depression (p \< 0.05). The severity of OA knee and QoL in the mental health aspect was a significant difference in patients in each age group. (p \< 0.01 and p \< 0.05, respectively). Depression and QoL were not correlated. The conclusion was patients had less severity of osteoarthritis, good QoL, and no anxiety or depression. Residential areas had no impact on QoL but healthcare providers should explain the treatment plan. The next study should focus on the long term of the patient's QoL.

Abstract The conclusions of the EFSA following the peer review of the initial risk assessments carried out by the competent authority of the rapporteur Member State, the United Kingdom, for the pesticide active substance Purpureocillium lilacinum strain PL11 and the considerations as regards the inclusion of the substance in Annex IV of Regulation (EC) No 396/2005 are reported. The context of the peer review was that required by Regulation (EC) No 1107/2009 of the European Parliament and of the Council. The conclusions were reached on the basis of the evaluation of the representative uses of Purpureocillium lilacinum strain PL11 as nematicide on tomato, pepper, aubergine, cucumber, courgette, melon, watermelon, pumpkin (field and greenhouse applications). The reliable endpoints appropriate for use in regulatory risk assessment are presented. Missing information identified as being required by the regulatory framework is listed.

Дослідження спрямоване на окреслення взаємозв’язків теорії та практики арґументації і теорії ухвалення рішень. Фокус уваги спрямований на виявлення специфіки модусів мультимодальної арґументації, що передбачає послідовне розв’язання таких завдань: а) доповнити та уточнити дефініцію терміна «арґументація» у контексті застосування арґументів в процесі ухвалення рішень; б) розвести смислове поле термінів «переконувати» та «запевняти» в контексті само-арґументації (self-argumentation); в) висвітлити роль особистісних особливостей дієвця у процесі вибору; г) дослідити особливості вербальних та візуальних арґументів у процесі ухвалення рішень. Автор виходить із браку досліджень, присвячених прикладному застосуванню теоретико-арґументативного наробку у процесі ухвалення рішень. Прийоми та методи арґументації пропонується розглянути крізь призму само-арґументації, де суб’єкт шукає підстави та надає докази для переконання і запевнення самого себе. Актуалізація цієї проблематики дає можливість залучити теоретичні здобутки філософії дії та активності, феноменології, когнітивних та комп’ютерних наук з метою розширення поля дослідження та виходу за окреслені дисциплінарні межі. Для розгляду теоретико-методологічної та джерельної бази дослідження був використаний системний підхід. Для порівняння арґументативних теорій та підходів були застосовані компаративний метод та метод аналогії. Автором обґрунтована необхідність прийняття до уваги теоретико-арґументативного наробку в контексті теорії ухвалення рішень. Дослідження на межі цих царин зумовлюють актуалізацію проблеми людини, як дієвця, як автора власних рішень, на шляху до яких постає необхідність пояснення та арґументації. Була висунута та обґрунтована гіпотеза щодо розгляду суб’єкта як одночасно адресанта і адресата в арґументативному процесі, що дало можливість підняти питання само-арґументації. У процесі останньої було проаналізовано місце різних модусів арґументації, зокрема вербального та візуального, а також наведено приклади сфер їхнього прикладного застосування. Наукові наробки в арґументативній царині відрізняються прикладним застосуванням. Уміння будувати, аналізувати та виокремлювати арґументи дають змогу не лише ретроспективно поглянути на арґументативний процес та допомогти обґрунтувати вже ухвалене рішення, але й надати перевагу певній альтернативі безпосередньо у ситуації вибору. Діалогічний характер арґументативного процесу можна тлумачити крізь призму само-арґументації, у процесі якої використовуються не лише вербальні, а й візуальні аргументи. На підставі розглянутих першоджерел продемонстровано, яким чином візуалізація інкорпорується в процес ухвалення рішень.

Considering the biological and ecological importance of Cu–thiol interactions and the discrepancies in previous research, this study focuses on Cu interactions with biologically and ecologically relevant thiols: glutathione (GSH), L-cysteine (L-cys), 3-mercaptopropionic acid (MPA), and thioacetic acid (TAA) in aqueous solution. The addition of Cu(II) to a thiol-containing solution led to a rapid reduction of Cu(II) and the formation of a Cu(I)–thiol complex. The mechanism of Cu(II) reduction and Cu(I) complex formation as well as the kinetics of Cu(I) oxidation strongly depend on the structural properties of the individual thiols investigated. The reducing power of the investigated thiols can be summarized as follows: L-cys ≅ GSH > MPA > TAA. The reaction order, with respect to Cu(I) oxidation, also changes over the time of the reaction course. The deviation of the reaction kinetics from the first order with respect to Cu(I) in the later stages of the reaction course can be attributed to a Fenton-like reaction occurring under low thiol concentration conditions. At high Cu:thiol ratios, in the case of GSH, L-cys, and MPA, the early stage of the reaction course is characterized by high Cu(I) stability, most likely as a result of Cu(I) complexation by the thiols present in excess in the reaction mixture.

Phosphoinositides (PIs) are small, phosphorylated lipids that serve many functions in the cell. They regulate endo- and exocytosis, vesicular trafficking, actin reorganization, and cell mobility, and they act as signaling molecules. The most abundant PIs in the cell are phosphatidylinositol-4-monophosphate (PI4P) and phosphatidylinositol-4,5-bisphosphate [PI(4,5)P2]. PI4P is mostly localized at the Golgi apparatus where it regulates the anterograde trafficking from the Golgi apparatus to the plasma membrane (PM), but it also localizes at the PM. On the other hand, the main localization site of PI(4,5)P2 is the PM where it regulates the formation of endocytic vesicles. The levels of PIs are regulated by many kinases and phosphatases. Four main kinases phosphorylate the precursor molecule phosphatidylinositol into PI4P, divided into two classes (PI4KIIα, PI4KIIβ, PI4KIIIα, and PI4KIIIβ), and three main kinases phosphorylate PI4P to form PI(4,5)P2 (PI4P5KIα, PI4P5KIβ, and PI4P5KIγ). In this review, we discuss the localization and function of the kinases that produce PI4P and PI(4,5)P2, as well as the localization and function of their product molecules with an overview of tools for the detection of these PIs.