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Objective To evalute the effects of intraoperative dexmedetomidine in adult patients during recovery periods from general anesthesia after lobectomy. Methods Fifty adult patients undergoing lobectomy were randomized into two groups: control group （Group A，n＝25） and dexmedetomidine group （Group B，n＝25） . No differences in baseline characteristics were observed between the groups. All patients were given propofol and remifentanil for anesthetic induction and maintenance. Ten minutes before the anestheia， patients were given dexmedetomidine （1 μg/kg over 10 minutes followed by a continuous infusion of 0.3 μg/kg/h throughout surgery until 10 minutes before the end of operation） or comparable volumes of saline as a placebo. After the surgery, all patients were sent to the post-anesthetic care unit （PACU） with intubation. The parameters including mean arterial pressure （MAP），heart rate （HR），arterial oxygen pressure （PaO2），bispectral index （BIS），respiratory rate （f），tidal volume （TV），sedation-agitation score （SAS），recovery time，and the stay of PACU were recorded. The times of requiring fentanyl and the VAS score were recorded too．Results （1） The MAP and HR in the group B was significantly lower in group B than that in group A （P<0.05） . （2） The BIS and f in the group B was significantly lower in group B than that in group A （P<0.05）， and the TV and PaO2 in group B was significantly higher than that in group A （P<0.05） （3） The SAS score decreased in the group B compared with the group A （P<0.05）.（4） The VAS score was significantly lower in group B than that in group A （P<0.05）， and in the group B， less patients needed fentanyl after extubation （P<0.05）. Conclusions Intraoperative dexmedetomidine administration can significantly reduce the blood pressure and heart rate in patients undergoing thoracic surgery， improve ventilation during recovery, and decrease the occurrence of agitation and postoperative analgesia during analepsia. [ABSTRACT FROM AUTHOR]

Objective To evaluate the safety and efficacy of dexmedetomidine in adult patients during thoractomy under general anesthesia. Methods Seventy-five adult patients undergoing thoractomy were randomized into two groups ： control group (Group A, n = 36) and dexmedetomidine group (Group B, n = 39). No differences in baseline characteristics were observed between the two groups. All patients received propofol and remifentanil for anesthetic induction and maintenance. Patients were given dexmedetomidine ( 1 μg/kg over 10 minutes followed by a continuous infusion of 0.3 μg/ kg/ h throughout surgery) or comparable volumes of saline as a placebo. The parameters including mean arterial pressure (MAP)，heart rate (HR)，bispectral index (BIS)， sedation-agitation scale (SAS), arterial oxygen pressure (PaO2), arterial oxygen saturation (SaO2) and the stay of post-anesthetic care unit (PACU) were recorded. The doses of propofol, remifentanil, atropine and phenylephrine were recorded too. Results Significantly lower MAP and HR were observed over time in the group B compared with the group A (P < 0.05, 0.01). Patients given dexmedetomidine required less propofol for anesthetic induction and maintenance ( P < 0.05) while required higher dosage of phenylephrine ( P < 0.05) during anesthesia. The SAS scores decreased in the group B (P < 0.05) compared with the group A. Significantly higher PaO2 were observed in the group B than in the group A (P < 0.05 ). Conclusions Dexmedetomidine administration can significantly reduce the requirement for propofol during anesthesia, decrease blood pressure and heart rate in patients undergoing thoractomy, and decrease the occurrence of agitation during analepsia, and also imporve the respiration. [ABSTRACT FROM AUTHOR]

Objective To explore the value of immunoglobulin in differential diagnosis of primary biliary cholangitis（PBC, hepatitis E and liver cirrhosis.Methods Blood samples were collected from 121 patients with PBC and 107 healthy people. The serum r-glutamyltransferase（r-GT, alkaline phosphatase（ALP, immunoglobulin IgG and IgM, total cholesterol（TCH and lipoprotein（a[Lp（a] were detected by biochemical routine tests; Blood routine counted white blood cells（WBC and platelets（PC; The IgG and IgM antibodies of HEV were detected by enzyme-linked immunosorbent assay（ELISA. Results r-GT, ALP, IgG and IgM in PBC patients increased significantly（P＜0.05 accompanied by significant decrease in WBC and PC values（P＜0.05. In patients with PBC cirrhosis, IgG increased significantly（P＜0.05, while WBC and PC values decreased significantly（P＜0.05. IgG and IgM in patients with PBC+cirrhosis+HEV antibody positive increased significantly（P＜0.05,while WBC and PC values decreased significantly（P＜0.05. Conclusions The increase of IgG and IgM might be used as diagnostic markers for the development of cirrhosis in PBC patients with HEV infection. [ABSTRACT FROM AUTHOR]

Copyright of Historical Research / Lishi Yanjiu is the property of Shehui Kexue Zazhishe and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

Red yeast is a general term for a class of red pigment-producing yeast, and they are widely distributed in various ecological environments such as soil, sea, rivers and lakes. Red yeasts have the ability to produce polysaccharides, oils, carotenoids and other active substances and thus own great potential for applications in agriculture, food, medicine and cosmetics. In recent years, studies on red yeast including their applications, genome sequencing and key metabolic enzymes have expanded the in-depth knowledge of this important group of yeast. In addition, progress has been made in genome editing and metabolic engineering modification of these yeasts, providing basis for developing more efficient red yeast strains for industrial applications. Here, we review the applications of red yeast and related research advances in genomic studies and metabolic engineering. This review would be helpful for better understanding the physiology and metabolic features of red yeast, as well as utilization of renewable biomass for sustainable bioproduction using red yeast. [ABSTRACT FROM AUTHOR]

To address the challenge of inadequate structural strength in the spatial framework of oil-based drilling and completion fluids under ultra-high temperatures and extended durations, which leads to poor solid phase carrying and suspension capabilities, two key materials were developed: an amphiphilic multiblock polymer viscosifier (HT-TQ) and an oil-soluble small molecule gelling agent (HT-CB). HT-TQ effectively enhances the yield point and low shear rate viscosity of the base emulsion, while HT-CB significantly improves the static yield point. Sepiolite fibers were selected as suspension enhancers, which, in synergy with HT-TQ and HT-CB, further improve the rheological properties of the emulsion after ultra-high temperature rolling and strengthen the spatial framework structure. Using these three suspension stabilizing materials as the core, optimal additives were selected to construct a highly stable oil-based drilling fluid system suitable for ultra-high temperatures. This system withstands temperatures up to 240℃, maintains a viscosity retention rate greater than 78% after five days of continuous ultra-high temperature rolling, with a yield point greater than 5 Pa and LSYP greater than 3 Pa, and exhibits excellent rheological properties under high temperature and high pressure. Additionally, by using compounded barite as a weighting material, a stable oil-based completion fluid system was developed, which withstands temperatures up to 240 ℃ and remains homogeneous without hard settling after ten days of static exposure to ultra-high temperatures, with a settlement degree less than 1.2 N. These research findings provide technical support for efficient drilling and completion fluids in deep and ultra-deep oil and gas reservoirs. [ABSTRACT FROM AUTHOR]

Copyright of Journal of Kunming Medical University / Kunming Yike Daxue Xuebao is the property of Kunming Medical Journal and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

Helium exists widely in the implosion process of nuclear fusion and cosmic stars in the warm dense state. Its thermodynamic properties and radiative transport parameters play an important role in the design of fusion experiments and the study of star structure evolution. In this paper, the quantum Langevin molecular dynamics method, which fully considers the physical effects of electron and ion collisions in warm dense matter, is used to simulate the response characteristics of helium ions and electrons in a wide ρ-T region. The state of equation database and electronic thermal conductivity database of warm dense helium in the temperature range of 10-60 kK and density range of 1-24 g/cm³ are constructed, and the radiative opacity of warm and dense helium at this temperature density is calculated. The results of this paper can provide necessary input parameters for fusion physics research and modeling of many basic astrophysical problems. [ABSTRACT FROM AUTHOR]

Based on the theory of evidence-based nursing ability, this paper summarized present situation and influencing factors of evidence-based practice ability of nurses in Traditional Chinese Medicine hospitals. In perspectives of practice, attitude, knowledge skill and relationship between evidence-based nursing skills and various influencing factors, a multi-level analysis on research progress of nurses’ evidence-based nursing ability was conducted, aiming to provide guidance and reference to promote the development of evidence-based practice ability and related training programs for nurses in Traditional Chinese Medicine hospitals. (以循证护理能力理论为基础, 对中西医结合医院护士循证实践能力现状及影响因素进行概述, 分别从循证实践行为、循证实践态度、循证知识技能及循证护理能力水平与各影响因素之间的关系, 多层次分析中西医结合医院护士循证护理能力研究进展, 为促进中医院护士强化循证实践能力及相关培训方案的制定提供指导性意见与参考。)

In order to solve the tunnel construction problems such as low efficiency and high consumption of rock tunnel boring ma, chine (TBM) in high-strength and high abrasion hard rock stratum, cutter head trapped in fault fracture zone stratum, and shield stuck in high stress extrusion stratum. Based on the interaction between TBM disc cutter, cutter head, shield and tunnel surrounding rock, through the statistical analysis of multiple TBM method tunnel engineering data, the functional relationships between disc cutter penetration index and rock mass characteristic index, disc cutter crushing volume wear rate and rock mass characteristic index, TBM equipment utilization and fault width, relative deformation of surrounding rock and strength stress ratio were revealed, and the multi-dimensional TBM adaptability evaluation method was established, which was based on disc cutter excavability and abrasion resistance, the risk of cutter head trapped and shield stuck. The research results provide a quantitative basis for TBM construction method selection, equipment design and solution of construction problems in complex geological tunnels. [ABSTRACT FROM AUTHOR]

试验探讨将复合酶制剂对泌乳中后期奶水牛乳成分和产奶量的影响。选取体况良好且泌乳胎次、泌 乳月、泌乳量基本一致的泌乳水牛36 头，随机分为4 组，每组9 个重复，每个重复1 头水牛。对照组奶水牛饲喂 基础日粮，试验Ⅰ组、Ⅱ组、Ⅲ组在基础日粮中分别添加2、5、10 g/(头·d)复合酶制剂。试验期42 d。结果显 示：试验组奶水牛的乳蛋白、乳非脂固形物、乳糖的含量及产奶量均与对照组差异不显著（P>0.05）。试验第42 d， 试验Ⅱ组、Ⅲ组奶水牛乳脂肪及乳总固形物含量显著高于对照组（P<0.05），乳脂肪含量分别提高23.55% 和 25.26%，乳总固形物含量分别提高16.71% 和17.44%。研究表明，奶水牛日粮中添加5~10 g 复合酶制剂，能够在一 定程度上改善水牛奶品质，减缓产奶中后期奶水牛产奶量下降的情况。 [ABSTRACT FROM AUTHOR]

To explore the influence of different reduced beam section connections on progressive collapse resistance of steel frame structure under fire, first of all, pushdown methods of two-story two-span steel frame beam-column substructure with middle column failure under normal temperature were carried out, and the resistance curves and failure modes in the collapse process were studied. The test results show that the middle column failure steel frame structure mainly relies on the bending mechanism and the catenary mechanism to resist the external load, and the catenary mechanism has hysteresis. Then, on the basis of the test, the two-story plane steel frame model was established by ABAQUS, and the quasi-static simulation at room temperature and fire was carried out. The progressive collapse resistance analysis was carried out on the four beam reduced section connections of RBS (reduced beam section), DRBS (double reduced beam section), OBW (opening beam web) and OBF-2 (opening beam flange). The FEM (finite element model) results show that RBS and DRBS can provide higher vertical resistance and deformation capacity than the unreduced model at both room temperature and high temperature. Compared with other reduced beam section connections, DRBS has the best deformation ability considering catenary effect. [ABSTRACT FROM AUTHOR]

公眾授權條款的推動是貫徹自由文化運動的重要方法與策略，然而，由於不同種類授權條款彼此間存在著不相容的問題，自由文化運動正面臨著資訊、社群、創作人及個別著作被割裂的危機。本文以自由文化運動中最重要的兩種授權條款——創用CC 授權條款及GFDL——為核心，析述授權不相容對於協力創作及重新混用創作方式所帶來之負面影響，並站在授權人之立場，觀察兩種授權條款之差異性。此外，本文亦介紹兩種授權條款近年來在促進彼此相容所作之努力及其侷限，同時就自由文化運動及授權條款相容性未來的長期發展，提出相關建議。 Promoting public licenses has been a crucial approach and strategy to free culture movement supporters. These licenses not only enlarge the scope of intellectual commons, but also reinforce the value and social norms underlying the movement. Nonetheless, as more and more public licensees emerge in the free culture communities, the freedom granted by these licenses is not elevated accordingly. On the contrary, intellectual commons, communities, and creators are increasingly fragmented because of the incompatibility between different public licenses. Using two of the most important licenses—Creative Commons licenses and GNU Free Documentation License—in free culture movement as examples, this Article illustrates how license incompatibility has become an obstacle for peer production and remixing creativity. This Article also analyzes the subtle differences between the two aforementioned licenses. Such analysis may help explain dissimilar preferences of a wide range of licensors and why various licenses simultaneously exist in the free culture communities. Furthermore, this Article provides a strategic guide for communities to solve the incompatibility problem.

BACKGROUND: In osteoporosis patients, the stability of cervical spine after internal fixation is poor; the fracture healing process is slow; and the risk of refracture at the same site and other sites is significantly increased. Therefore, it is necessary to design a new method of anterior internal fixation to reduce complications and surgical revision rate. OBJECTIVE: To design a new fixation system for the treatment of cervical spondylosis with osteoporosis through the anterior pedicle approach using threedimensional (3D) finite element analysis and 3D printing technology, which is beneficial to improve the stability of the patient’s cervical spine after surgery METHODS: 3D CT data of 15 healthy cervical spine patients and 15 osteoporosis patients who were examined in the Department of Orthopedics, Affiliated Central Hospital of Shenyang Medical College from December 2017 to December 2019 were collected. The cervical spine models of 30 patients were constructed by 3D printing technology. A new nail plate internal fixation system was designed based on the pedicle positioning guide. 3D models were subjected to the vertebral pedicle fixation through the anterior approach guided by the localizer under external conditions. The prepared postoperative models were connected to the computer for finite element analysis and biomechanical analysis, and the best nail plate internal fixation system was designed based on the analysis results. RESULTS AND CONCLUSION: (1) 3D printing technology was used to successfully construct the cervical spine models of osteoporosis patients, as well as the anterior vertebral pedicle fixation and positioning guide and the anterior vertebral pedicle fixation nail plate system. (2) Finite element biomechanics analysis results showed that the bone mass of patients with severe osteoporosis was significantly higher than that of normal bone by torsional displacement and rotation angle. The addition of screws did not significantly improve the torsional deformation of the anterioposterior bending and the left and right bending. The addition of screws had a significant improvement in the deformation of the axial torsional load. (3) The results indicated that the trial designed a vertebral pedicle internal fixation nail plate system for the anterior cervical approach using 3D printing technology, confirming that 3D printing technology can successfully assist the cervical spine through anterior pedicle fixation for cervical spondylosis combined with osteoporosis. [ABSTRACT FROM AUTHOR]

由於網際網路與數位科技的快速發展，數位匯流已經成為通訊傳播產業發展的主要潮流。數位匯流在技術上代表著不同數位產品間的功能整合；在產業面，則象徵著不同通訊傳播產業間的垂直整合。就管制面而言，數位匯流顛覆了原本以傳輸技術為區分基礎的通訊管制體系，並引發論者關於多媒體事業濫用市場力的疑慮。我國甫通過通訊傳播基本法及國家通訊傳播委員會組織法，並於今年成立國家通訊傳播委員會，以因應數位匯流的挑戰。 本文除討論國家通訊傳播委員會未來可能面對的管制挑戰外，並析述分層模式及技術中立原則等與數位匯流相關的管制思維。 With the advancement of the Internet and digital technologies, digital convergence has become a significant trend in the communications industry. In terms of technology, digital convergence means the integration between different digital products; it also symbolizes the vertical integration between different communications industries. Nonetheless, digital convergence has brought rigorous challenges to the existing regulatory framework, which divides up the landscape based on traditional services and technologies. Moreover, such convergence may enable businesses to further leverage their market power to neighboring markets, and, thus, lead to antitrust concerns. In order to cope with regulatory issues with regard to digital convergence, the Taiwanese government just enacted Communications Basic Law, and established the National Communications Commission (NCC). This paper not only portraits the challenges facing NCC, but also introduces some potential regulatory responses, such as layered model and technology neutrality doctrine.

Objective To investigate the predictive value of serum Lipocalin-2（LCN2）and Cystatin-C（Cys C）levels in acute pancreatitis（AP）complicated with acute kidney injury（AKI）. Methods Totally 204 patients with AP were selected（observation group）, and they were divided into the mild cases of 80, moderate cases of 51 and severe cases of 73 according to the degree of illness. Another 70 healthy volunteers（control group）were selected at the same time. Peripheral venous blood was collected to detect serum LCN2 and Cys C. The levels of serum LCN2 and Cys C in the observation group, control group and AP patients with different disease degrees were compared. The clinical and laboratory data of AP patients were collected, and the risk factors for AP complicated with AKI were analyzed by multivariate Logistic regression model. The predictive value of serum LCN2 and Cys C levels for AP complicated with AKI was evaluated by receiver operating characteristic（ROC）curve. Results The serum levels of LCN2 and Cys C in the observation group were significantly higher than those in the control group（both P<0. 05）. With the aggravation of AP patients' condition, the levels of serum LCN2 and Cys C increased gradually（both P<0. 05）. Among 204 AP patients, there were 48 patients with AP complicated with AKI, and 156 AP patients without AKI. Univariate analysis showed that the length of hospital stay, 24-hour urine volume, amylase, serum creatinine（Scr）, blood urea nitrogen（BUN）, C-reactive protein, procalcitonin, LCN2, and Cys C were related to AP complicated with AKI（all P<0. 05）. Multivariate Logistic regression analysis showed that 24-hour urine volume was the protective factor of AP complicated with AKI, and Scr, BUN, LCN2, and Cys C were the risk factors for AP complicated with AKI（all P<0. 05）. ROC curve analysis showed that serum LCN2 and Cys C levels had a certain predictive value for AP complicated with AKI, and the combined detection of the two levels had higher predictive value（P<0. 05）. Conclusion The serum levels of LCN2 and Cys C increase significantly in AP patients, which are independent risk factors for AP complicated with AKI; the serum levels of LCN2 and Cys C have certain predictive value for AP complicated with AKI, but their combined predictive value is higher. [ABSTRACT FROM AUTHOR]

Objective: To investigate the effect and mechanism of esophageal squamous cell carcinoma exosomal miR-181b-5p to the polarization of M2 macrophages. Methods: Extracted and identified exosomes from esophageal squamous cell carcinoma， and detected the expression of miR-181b-5p in esophageal squamous cell carcinoma cells and their exosomes by qRT-PCR. M0 type macro-phages were divided into PBS group， HEEC exo group， Eca-109 exo group， miR-NC exo group， miR-181b-5p exo group， miR-NC group， miR-181b-5p mimic group， si-NC group， si-PTEN group， miR-181b-5p exo+PTEN group. qRT-PCR was used to detect the expressions of CD163， CD206， iNOS and TNF-α in each group. The targeting relationship between miR-181b-5p and PTEN were veri-fied by double luciferase reporter gene experiment. Results: miR-181b-5p was significantly overexpressed in esophageal squamous cell carcinoma cells TE-13， TE-12， TE-10， Eca-109， KYSE30 and their exosomes （P<0.001）. Compared with miR-NC group， the expression of CD163 and CD206 in cells were significantly upregulated in the miR-181b-5p mimic group， as well as the expressions of iNOS and TNF-α were significantly downregulated （P<0.001）. The results of double luciferase reporter genes showed that PTEN was the target gene of miR-181b-5p. Compared with si-NC group， the expressions of CD163 and CD206 in cells were significantly upregu-lated in the si-PTEN group， as well as the expressions of iNOS and TNF- α were significantly downregulated （P<0.001）. Compared with PBS group， the expressions of CD163 and CD206 in cells were significantly upregulated in the Eca-109 exo group， as well as the expressions of iNOS and TNF- α were significantly downregulated （P<0.001）. Compared with miR-NC exo group， the expressions of CD163 and CD206 in cells were significantly upregulated in the miR-181b-5p exo group， as well as the expressions of iNOS and TNF-α were significantly downregulated （P<0.001）. Compared with miR-181b-5p exo group， the expressions of CD163 and CD206 in cells were significantly downregulated in the miR-181b-5p exo+PTEN group， as well as the expressions of iNOS and TNF-α were significantly upregulated（ P<0.001）. Conclusion: Exosomal miR-181b-5p inhibits PTEN expressions to promote M2 macrophage polarization. [ABSTRACT FROM AUTHOR]

Copyright of Shandong Medical Journal is the property of Shandong Medical Health Newspapers and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

Objectives: The development of high definition (HD) map is of paramount importance in advancing the digital infrastructure of transportation and serves as a fundamental component in creating the “data brain” of intelligent vehicles and smart cities. Furthermore, it promotes the growth of intelligent logistics networks and traffic safety risk monitoring systems. Methods: The existing HD map models are analyzed from the perspective of map information transmission models, and the concept of constructing HD map models based on map cognition mechanisms is introduced. It also provides an overview of domestic and international standards for HD map formats. Moreover, the principles to be followed in the compilation of HD map for intelligent driving in China are outlined. Additionally, key technologies involved in HD map production and updates are explored, with a particular focus on map collection, processing, and verification. The current application status of HD map is analyzed through the examination of examples such as vehicle planning and perception. Results: The development of HD map has made significant progress, but it also encounters difficulties and challenges. Existing HD map models tend to be complex and lack sufficient representation of dynamic elements, resulting in difficulties in maintaining real-time accuracy. Additionally, the production capacity of HD map remains insufficient to meet the demands of development, and challenges related to geographic information security and limited application depth persist. Conclusions: A concept of establishing a closed-loop architecture for HD map using cognitive models is introduced. It highlights the significance of ensuring data security and promoting intelligent verification of maps and emphasizes the extensive application prospects of high-precision maps. During the research process of HD map, it is crucial to collaboratively address the difficulties and challenges, which can promote HD map to better serve intelligent transportation and societal development. [ABSTRACT FROM AUTHOR]

Objective To investigate the mechanism and the effect of miR-524-5p regulating HEG1 expression on the proliferation and epithelial-mesenchymal transition of esophageal cancer cells. Methods The expression levels of miR-524-5p and HEG1 mRNA in esophageal cancer cells and normal esophageal epithelial cells were detected by qRT-PCR. KYSE30 cells were divided into miR-524-5p mimic group, miR-524-5p NC group, miR-524-5p mimic+pcDNA3.1 group, and miR-524-5p mimic+pcDNA3.1- HEG1 group. Non-transfected cells were set as the normal control group (group Control). CCK-8 method was applied to detect the proliferation ability of KYSE30 cells. Western blot analysis was conducted to detect the expression of proteins related to EMT, invasion, and migration and the HEG1 protein. Scratch and Transwell assays were applied to detect the migration and invasion abilities of KYSE30 cells. A dual-luciferase reporter gene was used to examine the targeting relationship between miR-524-5p and HEG1. Results miR-524- 5p was lowly expressed in four esophageal cancer cell lines, namely, TE-1, KYSE30, KYSE150, and NEC (P<0.05). KYSE30 cells with the lowest expression level were selected for subsequent experiments. HEG1 mRNA was highly expressed in four esophageal cancer cell lines (P<0.05). The GEPIA database showed that HEG1 was highly expressed in esophageal cancer tumor tissues (P<0.05). KYSE30 cells in the miR524-5p mimic group had lower proliferation ability, colony formation number, mesenchymal marker protein expression, and migration and invasion abilities and upregulated epithelial marker protein E-cadherin level than cells in the miR-524-5p NC group (P<0.05). The miR-524-5p mimic+pcDNA3.1-HEG1 group significantly reversed the inhibitory effect of overexpression of miR-524-5p on the proliferation, epithelial– mesenchymal transformation, invasion, and metastasis of KYSE30 cells (P<0.05). The luciferase activity of cells in the miR-524-5p mimic and WT-HEG1 co-transfection groups was lower than that in the miR-524-5p NC and WT-HEG1 co-transfection groups (P<0.05). Conclusion miR-524-5p is lowly expressed in EC cells and tissues. The overexpression of miR-524-5p can negatively regulate the expression of HEG1 in esophageal cancer cell line (KYSE30 cells) and reduce the proliferation, EMT process, and invasion and migration abilities of KYSE30 cells. [ABSTRACT FROM AUTHOR]

Objective: To explore the molecular mechanism of prostate cancer bone metastasis by bioinformatics, and to provide a new target for prevention and treatment of prostate cancer bone metastasis. Methods: The dataset GSE74685 containing 20 cases of prostate cancer bone metastasis and 69 cases of prostate cancer lymph node metastasis were retrieved and screened from the Gene Expression Database （GEO）. The Limma package of R software is used to preprocess the original data and screen differentially expressed genes （DEGs）. DEGs were analyzed by GO function enrichment and KEGG pathway enrichment analysis through the gene function annotation online tool DAVID. The protein-protein interaction （PPI） network of DEGs was constructed by using the STRING online retrieval tool and Cytoscape software. And the important modules and key genes were screened by using MCODE and cytoHubba plug-ins. The expression, prognosis and diagnostic value of key genes were verified by HCMDB and GEPIA database. Results: A total of 2 022 DEGs were selected from the dataset GSE74685, including 842 up-regulated genes and 1 180 down-regulated genes. GO functional enrichment analysis showed that DEGs were mainly enriched in the extracellular matrix organization, collagen catabolic process, response to mechanical stimulus, and positive regulation of neutrophil chemotaxis. KEGG pathway enrichment suggested that focal adhesion and ECM-receptor interaction were the main signal pathway for DEGs enrichment. Eleven key genes, EZH2, PLG, SRC, CAV1, CHD4, HIST2H2BE, KDM1A, POLR2E, VWF, APOE and THBS1 were obtained through PPI network screening. The verification of gene expression and survival analysis of 11 key genes showed that APOE and EZH2 were closely related to prostate cancer bone metastasis. Conclusion: EZH2 and APOE may be involved in the occurrence and development of prostate cancer bone metastasis, which can be used as potential therapeutic targets and provide a new research direction for the prevention and treatment of prostate cancer bone metastasis. [ABSTRACT FROM AUTHOR]

At present, the research on the flushing efficiency of drilling fluid filter cake in cementing engineering mainly focuses on the flushing condition of the circular hole casing in the middle, while the research on the influence of the hole shape and casing eccentricity on the flushing efficiency of drilling fluid filter cake is less. This study uses the drilling fluid filter cake flushing simulation experimental device to test the influence of eccentricity and flushing flow rate in the elliptical hole on the drilling fluid filter cake flushing efficiency, and analyzes the flow field of the flushing fluid in the annulus based on the computational fluid dynamics (CFD) method, calculates the hydraulic shear force of the flushing fluid, and clarifies the mechanism of the change of the filter cake flushing efficiency. The results show that when the short axis direction in the annulus of the elliptical hole is eccentric, the flushing efficiency of the drilling fluid filter cake is the largest in the long axis direction of the annulus, followed by the wide and short axis direction, and the narrow and short axis direction is the smallest; By increasing the eccentricity of the short axis in the annulus, the flushing efficiency of the drilling fluid filter cake decreases slightly in the long axis direction, increases in the wide and short axis direction, and decreases significantly in the narrow and short axis direction; With the increase of annulus flushing flow, the overall flushing efficiency of annulus drilling fluid filter cake is significantly increased, but the increase of filter cake flushing efficiency in the long axis direction of the annulus is the largest, followed by the wide and short axis, and the narrow and short axis is the smallest. [ABSTRACT FROM AUTHOR]

Objective To evaluate the analgesic effect of ultrasound-guided transmuscular quadratus lumborum block in pediatric patients for hip surgery. Methods 60 children with elective hip operation, aged 1-3 years, ASAⅠ ～Ⅱ, were randomly divided into 2 groups after anesthesia induction tracheal intubation. The patients of TQL group （n = 30) received ultrasound-guided transmuscular quadratus lumborum block, while the patients of group C （n = 30） did not receive TQL block. The primary outcome was the number of patients who require rescue analgesia in the first 24 h. The secondary outcome were the FLACC pain scale, heart rate, and blood pressure at 1 h, 4 h, 6 h, 12 h and 24 h postoperatively, and postoperative adverse event （e.g., local hematoma, and organ injuries). Results 24 h after surgery, the need for rescue analgesia was statistically significantly higher in group C when compared to the TQL group [21/30 （70%） vs 5/30 （16.7%), P < 0.001]. The FLACC score, heart rate, and blood pressure were lower in the TQL group than the C group at various points during 24 h postoperatively （P < 0.05）. No postoperative adverse event occurred in the TQL group. Conclusion Ultrasound guided quadratus lumborum block reduces pain scores and analgesic needs following hip surgery in pediatric patients. [ABSTRACT FROM AUTHOR]

Objective To investigate the correlation between stress hyperglycemia ratio (SHR) and 90-day clinical prognosis of patients with acute ischemic stroke (AIS). Methods The data of AIS patients who were admitted in Department of Neurology, Yangpu Hospital, Tongji University from May 2016 to October 2020 were retrospectively analyzed. The outcome was assessed by 90-day mRS and composite vascular events (recurrent stroke, myocardial infarction and vascular death). The favorable prognosis was defined as a mRS score of 0-2, and unfavorable prognosis was defined as a mRS score of 3-6 and composite vascular events within 90 days after stroke. SHR was calculated by fasting blood glucose divided by the estimated average blood glucose. The multivariate logistic regression analysis was used to determine whether SHR was an independent influencing factor of the 90-day prognosis. ROC curve was used to evaluate the effect of SHR to predict 90-day prognosis. Spearman correlation was used to analyze the correlation between SHR and clinical factors. Results (1) A total of 1484 patients were included,with the median age of 70 (62-80) years and 948 males (63.9%). The median baseline NIHSS score was 3 (2-7). There were 923 cases (62.2%) in favorable prognosis group and 561 cases (37.8%) in unfavorable prognosis group. (2) Univariate analysis showed that the SHR in favorable prognosis group was significantly lower than that in unfavorable prognosis group [0.82 (0.72-0.95) vs . 0.86 (0.74-1.02), P =0.001]. (3) Multivariate logistic regression analysis showed that compared with the lowest quartile of SHR, the highest quartile was independently associated with the 90-day unfavorable prognosis (OR 2.22, 95%CI 1.26-3.91, P =0.006), which had statistical difference in non-diabetic group in subgroup analysis (OR 2.11, 95%CI 1.19-3.75, P =0.010), and no statistical difference in diabetic group (OR 1.53, 95%CI 0.69-3.42, P =0.298). (4) The area under the ROC curve (C-value) of SHR for predicting 90-day unfavorable prognosis was 0.552 (95%CI 0.526-0.578, P =0.001, the optimal cut-off value was 0.84, the sensitivity was 52.94%, and the specificity was 56.12%); and in non-diabetic subgroup, the C-value was 0.600 (95%CI 0.563-0.637, P

Bioactivities of eight fungicides (boscalid, difenoconazole, propiconazole, flusilazole, carbendazim, prochloraz, dimethachlon, mancozeb) to the pathogen of a tobacco leaf spot, Didymella segeticola, were determined using bioassay method at the mycelial growth stage. In vivo protective and curative effects of these fungicides against D. segeticolla on detached tabbaco leaves were also analyzed. The results showed that eight fungicides exhibited different inhibitory activities against mycelial growth of the pathogen, and also showed some protective and curative efficacies against the leaf spot. The most effecient one against mycelial growth of the pathogen was boscalid, with an EC50 value of (0.047 0 ± 0.012 0) mg/L; followed by difenoconazole [(0.079 0 ± 0.005 0) mg/L], prochloraz [(0.29 ± 0.08) mg/L], propiconazole [(0.69 ± 0.12) mg/L], dimethachlon [(1.08 ± 0.33) mg/L], carbendazim [(1.22 ± 0.29) mg/L], flusilazole [(1.38 ± 0.07) mg/L]; and most least effecient one was mancozeb with an EC50 value of (22.80 ± 10.51) mg/L. Flusilazole, difenoconazole, propiconazole, boscalid and dimethachlon all had better protective activity for the control of this disease with >82% efficacy at 25 mg/L dosage treatment than 100 mg/L mancozeb showed 70.51% efficacy. Both boscalid and flusilazole showed better curative activities, with efficacies of >80% at 25 mg/L dosage treatment, followed by prochloraz, difenoconazole, carbendazim and dimethachlon; mancozeb showed relatively poor activity with 63.31% efficacy at 100 mg/L dosage treatment. The results provide scientific basis and reference for the screening of fungicides to control tobacco leaf spot. [ABSTRACT FROM AUTHOR]

To study the mechanical properties and failure modes of corroded reinforced concrete beams under impact, this paper conducts impact tests on concrete beams by Fujikake et al. The validity of impact force time history curve, mid-span displacement time history curve and failure mode of the fine finite element model of reinforced concrete beam structure established by finite element software ANSYS/LS-DYNA was checked. Based on the verified finite element model, the influence of corrosion rate of reinforcement on the impact resistance of concrete beams was researched. The finite element analysis shows that The concrete spalling degree of concrete member under impact effect increases with the increase of steel corrosion rate. The higher the degree of steel corrosion, the more serious the concrete spalling. All the corroded RC beams at different impact heights exhibit flexural failure characteristics. Steel corrosion has a certain influence on the impact resistance of reinforced concrete members. At the same drop weight impact height, the peak impact force decreases with the increase of steel corrosion rate, and the mid-span displacement increases with the increase of steel corrosion rate. When the impact height is 3.0m, the peak value of impact force decreases to 17.9% and the mid-span displacement is 33.3% when the corrosion rate is 30.0%. [ABSTRACT FROM AUTHOR]

Objective: To compare the analgesic effect between ultrasound-guided single-dose adductor canal block with dexmedetomidine combined with ropivacaine and continuous femoral nerve block with ropivacaine after patella fracture surgery. Methods: 31 patients with patella fractures who underwent elective general anesthesia, with age ranging from 21 to 69, BMI ≤ 35 kg/m2, ASA grading Ⅰ～Ⅲ, were randomly divided into two groups. Single-dose adductor canal block group who received 100 μg dexmedetomidine combined with 0.25% ropivacaine 50 mg (A group, n = 21), and continuous femoral nerve block group who received 0.25% ropivacaine 250 mg (F group, n = 10). The scores regarding analgesia, sedation, and muscle strength at 6, 8, 12, 24, 36 and 48 h after surgery were observed and compared between the two groups and the additional postoperative analgesia and adverse reactions were recorded. Results: The analgesic effect, postoperative scores based on sedation and muscle strength were not statistically different between the two groups at various points during the observation period (P > 0.05) . Conclusion　Single-dose adductor canal block with dexmedetomidine combined with ropivacaine can provide good analgesia after patella fracture surgery. [ABSTRACT FROM AUTHOR]

Objective To investigate the clinicopathological features and prognosis of ovarian granulosa cell tumor (GCT) in children. Methods • The clinicopathologic and follow-up data of 8 patients with ovarian GCT in Shanghai Children's Hospital from June 2008 to June 2018 were collected and retrospectively studied to summarize the clinicopathological features and analyze the prognosis. Results • The age of onset in this group ranged from 2 to 12 years with median age of 6.5 years. Two cases were adult granulosa cell tumor (AGCT) and six cases were juvenile granulosa cell tumor (JGCT). The first symptoms were abdominal pain or abdominal mass. Six patients were complicated with symptoms of precocious puberty, including 5 cases of true precocious puberty and 1 case of pseudo precocious puberty. The levels of peripheral blood sex hormones changed to varying degrees. Gross examination showed that the tumor masses ranged from 4 to 22 cm in the greatest dimension (average 12.8 cm). All of the 8 tumors were mixed solid-cystic in appearance. Under light microscope, AGCT showed obvious nuclear sulcus and microfollicular structure, containing eosinophilic substances, namely characteristic Call-Exner bodies. JGCT had follicular structures of different sizes, containing basophilic secretions. The inner layer of the follicular wall was composed of granular cells, which could be surrounded by follicular membrane cells. No Call-Exner bodies were found, and nuclear sulcus were rare. The results of immunohistochemistry showed that a-inhibin and CD99 were positive, and epithelial membrane antigen (EMA) were negative in all the cases. In 5 cases calretinin were positive, and in 5 cases cytokeratin (CK) were positive. Ki-67 proliferation index was 5%-50%. There were 3 estrogen receptor (ER) positive cases and 6 progesterone receptor (PR) positive cases. Forkhead transcription factor 2 (FOXL2) was highly expressed in 2 cases of AGCT and 6 cases of JGCT. SRY-box transcription factor 9 (SOX9) positive cells were scattered in 2 cases of AGCT and 3 cases of JGCT. All the cases were treated with standard surgery. Tumor rupture or metastasis was found in 5 cases, and ascites was suspiciously positive in 1 case. Eight children with ovarian GCT were followed up for 19-155 months, and all of them survived in good health. Conclusion The clinical features of AGCT and JGCT in children's ovaries are similar and have typical histopathological features respectively. The selection of suitable immunohistochemical detection method is helpful for differential diagnosis of ovarian tumors in children. The prognosis of ovarian GCT in children is good even with tumor rupture after radical surgery. [ABSTRACT FROM AUTHOR]

In order to investigate the coal volumetric swelling during CO2 storage, the self-developed MCQ-ⅡCO2-ECBM experimental equipment was used to perform coal deformation through injecting CO2 to meager coal, meager lean coal and weakly caking coal samples （100 mm × 100 mm × 200 mm） with constant injection pressure under 31 MPa bulk stress at 25 ℃. At the same time, the micro-pores were investigated with nine small coal samples （6 mm×6 mm×6 mm） through a micro-CT system. The results show that: At minimum 4. 5 μm observation of CT section structure, the meager coal porosity （4. 67%） is less than that of meager lean coal （5. 39%） and weakly cakingcoal （12. 10%）; During 12 hours of CO2 injection, coal volumetric strain is influenced by pore pressure and CO2 adsorption swelling, which is linearly proportional to the amount of gaseousness CO2 storage under 31 MPa confinement pressure; With the same CO2 storage, the volumetric swelling strain of the meager coal is more than that of the weakly caking coal and meager lean coal; Due to the anisotropy of modulus of elasticity, the radial strain increases faster than the axial strain and both of them are linearly proportional to the amount of gaseousness CO2 storage. [ABSTRACT FROM AUTHOR]

Based on the unsaturated characteristic of medium-high rank coal reservoirs and coalbed methane (CBM) well production data, the material and energy dynamic balance mechanism in middle-high rank CBM systems was revealed by considering the critical desorption pressure as a key parameter. The control action of this mechanism on practical CBM development was also analyzed. Results show that four aspects of the requirements in the field can be realized through using above mechanism, including the real-time monitoring of reservoir pressure and water saturation, the single CBM well recoverable reserves calculation, the reservoir absolute or relative as well as effective permeability dynamic prediction and the productivity dynamic numerical simulation.The higher the relative gas content (the absorbed gas saturation) of the coal reservoirs is, the faster the reservoir pressure and water saturation decline, the easier the CBM desorption is. After the reservoir pressure is under a critical desorption pressure, the longer production time could bring more accurate reserves computation.In the whole process of CBM production, the coal reservoir permeability are unified as the function of reservoir pressure and the unsaturated effective permeability curves can better reflect the positive and negative effects as well as the gas slippage effect. Meanwhile, the unsaturated effective permeability model is more accurate and precise than that of the saturated effective permeability model in the CBM well productivity prediction. [ABSTRACT FROM AUTHOR]